Frontiers in Pharmacology
·
2023
·
IF 5.6
Yang Yang,Cuiting Lin,Qiang Zheng,Leqi Zhang,Yongmei Li,Qinghua Huang,Ting Wu,Zean Zhao,Lu Li,Jian Luo,Yanqing Jiang,Qun Zhang,Xing Wang,Chenglai Xia,Jianxin Pang
Hyperuricemia (HUA) is associated with left ventricular remodeling (LVR) and thereby causes the initiation and development of a large number of cardiovascular diseases. LVR is typically accompanied by cardiomyocyte energy metabolic disorder. The energy supply of cardiomyocytes is provided by glucose and fatty acid (FA) metabolism. Currently, the effect of HUA on cardiomyocytic FA metabolism is unclear. In this study, we demonstrate that UA-induced cardiomyocyte injury is associated with cytoplasmic lipid deposition, which can be ameliorated by the FA metabolism-promoting drug L-carnitine (LC). UA suppresses carnitine palmitoyl transferase 1B (CPT1B), thereby inhibiting FA transport into the mitochondrial inner matrix for elimination. LC intervention can ameliorate HUA-associated left ventricular anterior wall thickening in mice. This study showed that FA transport dysfunction plays is a critical mechanism in both cardiomyocytic injury and HUA-associated LVR and promoting cytoplasmic FA transportation through pharmacological treatment by LC is a valid strategy to attenuate HUA-associated LVR.
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ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Fanrong Zeng,Muhammad Mudassir Nazir,Temoor Ahmed,Muhammad Noman,Shafaqat Ali,Muhammad Rizwan,Mohammad Shah Alam,Jonas Lwalaba Wa Lwalaba,Guoping Zhang
Arsenic contamination in agricultural soils has posed tremendous threat to sustainable crop production and human health via food chain. Calcium and Glutamate have been well-documented in metal(loid)s detoxification, but it is poorly understood how they regulate arsenic-induced toxicity to plants. In this study, the effect of glutamate and calcium at high concentration on arsenic toxicity and accumulation in barley seedling was accessed in terms of plant growth, photosynthetic efficacy, arsenic uptake, translocation and accumulation, antioxidant defense, nutrient uptake and the expression of As transporters. Our results have demonstrated that calcium could effectively ameliorate arsenic toxicity to barley seedlings, which is mainly attributed to its beneficial effect on increasing nutrient uptake, reducing the aboveground arsenic accumulation and enhancing antioxidative defense capacity. However, it is unexpected that glutamate considerably exacerbated the arsenic toxicity to barley seedlings. More importantly, for the first time, glutamate was observed to tremendously facilitate the root-to-shoot translocation of arsenic by 41.8- to 60.8-fold, leading to 90% of the total amount of As accumulating in barley shoots. The reason of this phenomenon can be well explained by the glutamate-triggered enormous upregulation of genes involved in arsenic uptake ( HvPHT1;1 , HvPHR2 and HvNIP3;2 ), reduction ( HvHAC1;1 ), translocation ( HvABCC7 , HvNIP1;1 and HvNIP3;3 ) and intracellular sequestration ( HvABCC1 ). These findings suggest that calcium and glutamate function as the opposite player in managing arsenic, with calcium being an effective alleviator of arsenic stress to ensure the safe production of crops; while glutamate being a highly efficient phytoextraction agent for phytoremediation of arsenate-contaminated soils.
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CRYSTAL GROWTH & DESIGN
·
2022
·
IF 4.01
Liyu Liu,Zaiyong Zhang,Yinxiang Cheng,Yihua Jiang,Liye Lu,Jian-Rong Wang,Xuefeng Mei
L-menthol is one of the most important flavoring additives with abundant plant sources. The distinctive odor, cooling characteristic, and diversified biological activities attribute to its widespread application, especially on confections, oral-care commodities, over-the-counter drugs, and tobacco flavors. However, the manufacturing and storage of L-menthol face challenges due to its high volatility and low melting point. In this work, cocrystallization provides a new strategy to manage the stability problem of this important ingredient. Coformers of dietary sources were chosen based on a supramolecular synthon design, and six cocrystals of L-menthol were synthesized by either a solvent-mediated or solvent-free approach. These new solid forms could significantly increase the melting point and reduce the vapor pressure up to 1/45 of the pure L-menthol. Moreover, cocrystals present a high retention rate under storage conditions. The superior stability can be ascribed to diversified supramolecular cavities assembled by coformers, to which L-menthol molecules are restricted by hydrogen bonds.
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JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2023
·
IF 8.7
Yi Lu,Zhaoxue Zhang,Yingyang Wang,Fangyuan Peng,Zhaoguang Yang,Haipu Li
Boehmeria nivea L. (ramie) is a promising phytoremediation plant for antimony (Sb)-contaminated soils. However, the uptake, tolerance, and detoxification mechanisms of ramie to Sb, which are the basis for finding efficient phytoremediation strategies, remain unclear. In the present study, ramie was exposed to 0, 1, 10, 50, 100, and 200 mg/L of antimonite (Sb(III)) or antimonate (Sb(V)) for 14 days in hydroponic culture . The Sb concentration , speciation, subcellular distribution, and antioxidant and ionomic responses in ramie were investigated. The results illustrated that ramie was more effective in the uptake of Sb(III) than Sb(V). Most of the Sb accumulated in ramie roots, with the highest level reaching 7883.58 mg/kg. Sb(V) was the predominant species in leaves, with 80.77–96.38% and 100% in the Sb(III) and Sb(V) treatments, respectively. Immobilization of Sb on the cell wall and leaf cytosol was the primary mechanism of accumulation. Superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) contributed significantly to root defense against Sb(III), while CAT and glutathione peroxidase (GPX) were the major antioxidants in leaves. CAT and POD played crucial roles in the defense against Sb(V). B, Ca, K, Mg, and Mn in Sb(V)-treated leaves and K and Cu in Sb(III)-treated leaves may be related to the biological processes of Sb toxicity mitigation. This study is the first to investigate the ionomic responses of plants toward Sb and could provide valuable information for the phytoremediation of Sb-polluted soils.
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Polymers
·
2022
·Vol. 14
·期 24
·5509
·
IF 4.967
Haoying Wang,Xiaoxia Song,Junfeng Xiong,U Kei Cheang
Magnetically actuated microrobots showed increasing potential in various fields, especially in the biomedical area, such as invasive surgery, targeted cargo delivery, and treatment. However, it remains a challenge to incorporate biocompatible natural polymers that are favorable for practical biomedical applications. In this work, bilayer magnetic microrobots with an achiral planar design were fabricated using a biocompatible natural polymer and Fe3O4nanoparticles through the photolithography by applying the layer-by-layer method. The microrobots consisted of a magnetic bottom layer and a photo-crosslinked chitosan top layer. The SEM results showed that the microrobot processed the L-shaped planar structure with the average width, length, and thickness of 99.18 ± 5.11 μm, 189.56 ± 11.37 μm, and 23.56 ± 4.08 μm, respectively. Moreover, microrobots actuated using a three-dimensional (3D) Helmholtz coil system was characterized and reached up to an average maximum velocity of 325.30 μm/s and a step-out frequency of 14 Hz. Furthermore, the microrobots exhibited excellent cell biocompatibility towards L929 cells in the CCK-8 assay. Therefore, the development of bi-layered chitosan-based microrobots offers a general solution for using magnetic microrobots in biomedical applications by providing an easy-to-fabricate, highly mobile microrobotic platform with the incorporation of biocompatible natural polymers for enhanced biocompatibility.Keywords:chitosan;microrobot;photolithography;natural polymer;magnetic actuation
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MOLECULES
·
2022
·Vol. 27
·期 24
·8858
·
IF 4.927
Gang Li,Ming Chu,Yingying Tong,Yuexin Liang,Shenghui Wang,Chengjun Ma,Zhenhua Wang,Wenna Zhou
Hippophae rhamnoidesL. is a deciduous shrub that contains many unique bioactive substances. This sea buckthorn possesses anticancer, antioxidant, anti-inflammatory, and cardiovascular protective properties. Herein, the effects of phenylpropyl compounds extracted fromH. rhamnoidesL. on doxorubicin (Dox)-induced cardiotoxicity were evaluated in zebrafish. Cardiac injury in zebrafish was induced using 35 μM Dox for 96 h, and 30 μM phenylpropanoid compounds were used as the protective treatment. The cardioprotective effects and mechanisms of the four phenylpropanoids were investigated using microscopy, behavioral analysis, acridine orange staining, western blotting, flow cytometry, and real-time quantitative polymerase chain reaction. The extracted phenylpropanoids could significantly relieve Dox-induced cardiac injury in zebrafish and inhibit cardiomyocyte apoptosis. The mechanisms of action were mainly related to the stability of mitochondrial biogenesis and function maintained by phenylpropanoids in zebrafish. To our knowledge, this is the first report on the protective effect of sea buckthorn against myocardial injury in zebrafish. Our findings provide support for the further research and development of sea buckthorn and its components.Keywords:sea buckthorn;zebrafish;doxorubicin;cardiotoxicity;mitochondria
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Materials Today Chemistry
·
2022
·
IF 7.613
W. Song,M. Zhang,X. Huang,B. Chen,Y. Ding,Y. Zhang,D.G. Yu,I. Kim
Porous organic polymers having well-defined morphologies and designable functionalities are potentially suitable materials for environmental remediation, e.g., for the removal of pollutants from water. However, single materials generally do not simultaneously possess high adsorption abilities and antibacterial performance, as well as easy processibility. Herein, we reported a processible porous polymer which composed swellable hollow spherical morphology and branched L-borneol antibacterial agents. These features endure a valuable capability of pollutant absorption for represented pollutants such as, organic dye (methylene blue), organic compound (bisphenol A), and the heavy metal (Cu 2+ ). In addition, the L-borneol groups and 3D crosslinked hierarchical structure result in enhancement of antibacterial performance against Escherichia coli and Staphylococcus aureus . Moreover, we demonstrate the preparation of the polymer as a fine powder could anchored on the membranes, processed into the tablet, adhered with the polyurethane foams, and assembled as the dye absorption device. The rationally designed polymers by this method show notable potential as adsorbents for water purification and sewage treatment.
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Foods
·
2022
·Vol. 11
·期 22
·3625
·
IF 5.561
Yusi Yang,Zhanbo Dong,Yongkang Wang,Fengyi Xiao,Jinliang Yang,Dong Zhao,Jianhui Ye,Xinqiang Zheng,Yuerong Liang,Jianliang Lu
Adsorption is an important technology for the separation of different tea components. The adsorption behavior of L-theanine onto adsorbents was comprehensively studied in this paper. Among tested adsorbents, cation exchange resin ZGSPC106Na and D001SD were suitable for separating L-theanine, PVPP and PA-6 for catechins and macroporous resin HPD-400 for caffeine. Adsorption of L-theanine onto the cation resins was significantly influenced by the acidity, contact time and temperature. The adsorption behavior could be described by the pseudo-second-order rate equation and fitted to Langmuir and Freundlich models. ZGSPC106Na exhibited higher adsorption capacity, while D001SD showed higher adsorption selectivity. These might be attributed to the distinctive structure of the two resins and different ionization of the adsorbates. A method for simultaneous preparation of decaffeinated polyphenols, caffeine-enriched extract and decaffeinated L-theanine was established through successive separation on the columns fulfilled with PA-6, HPD-400 and D001SD, respectively.Keywords:separation of tea components;amino aicd;decaffeination;adsorption capacity;adsorption selectivity;adsorbent
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Zhen-Hui Liu,Qing-Ming Wang,Qiu-Feng Lü,Junxiong Wu
Creating an efficient method that can provide simple, practical and high-throughput separation of oil–water mixtures has proved extremely challenging. Two-dimensional layered Ti 3 C 2 T x MXene material with excellent hydrophilicity has attracted substantial attention for oil–water separation. However, conventional Ti 3 C 2 T x separation membranes are susceptible to contamination by oil due to its relatively high adhesion to oil, hindering its practical applications in high-efficiency oil–water separation. To make Ti 3 C 2 T x have underwater superoleophobicity and low oil adhesion, the surface of Ti 3 C 2 T x can be modified by introducing hydrophilic groups. Herein, L-lysine functionalized Ti 3 C 2 T x (Ti 3 C 2 T x -Ly) was firstly prepared by decorating L-lysine on Ti 3 C 2 T x through electrostatic interaction and hydrogen bonding . A facile dip-coating method is then used to coat polyurethane (PU) sponge with Ti 3 C 2 T x -Ly, obtaining a superhydrophilic/underwater superoleophobic Ti 3 C 2 T x -Ly @PU sponge. Owing to the excellent underwater superoleophobicity resulting from hydrophilic groups (–OH、–NH 3 ) on the surface of Ti 3 C 2 T x -Ly nanosheets and the enlarged interlayer spacing of Ti 3 C 2 T x , the Ti 3 C 2 T x -Ly @PU sponges demonstrated outstanding separation performance for a series of oil–water mixtures. Specifically, the Ti 3 C 2 T x -Ly @PU sponge exhibits low oil adhesion and superhydrophilic (WCA = 0°)/submerged superoleophobic (UOCA > 155°) properties. The Ti 3 C 2 T x -Ly @PU sponges displayed the best separation performance for cyclohexane/water mixture, and the maximum permeation flux is up to 221892 L m –2 h –1 , accompanied by a remarkable separation efficiency of higher than 99.2% after 100 cycles. The permeation flux has exceeded most of the previous reported multifunctional separation membranes. In addition, the Ti 3 C 2 T x -Ly @PU sponge also showed excellent separation performance for high-density oil dichloromethane/water with a separation efficiency over 99.4%. Of note, the Ti 3 C 2 Tx-Ly @PU sponge features excellent chemical stability to corrosive media, such as acidic, alkaline, and high salt solutions, possessing great potential for real-world applications. Furthermore, the as-prepared Ti 3 C 2 T x -Ly @PU sponge exhibits rapid and efficient separation of oil-water mixtures through a fully gravity-driven process, which makes it a good candidate for industrial oil-contaminated water treatment and oil spill clean-up, and also provides new insights into the design and development of functional Ti 3 C 2 T x through L-lysine modification. Therefore, this work reports a facile and promising way to fabricate scalable superhydrophilic/underwater superoleophobic sponge material for highly efficient oil–water separation.
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Agriculture-Basel
·
2022
·Vol. 12
·期 5
·604
·
IF 3.408
Xing Han,Fei Yao,Ying Wang,Xinyao Duan,Zhilei Wang,Yihan Li,Tingting Xue,Xu Liu,Hua Wang,Hua Li
Biodegradable liquid film (BLF) improves soil structure and increases plant freezing tolerance after spraying on the surface of soil and plant. In this study, the effects of BLF on grape composition and volatile compounds in Cabernet sauvignon (Vitis viniferaL.) grapes were determined by spraying BLF during the dormant periods over three years. The aim of the study was to evaluate the potential impact of BLF as an overwintering protection measure on grape fruit quality. In 2020 and 2021, BLF spraying increased reducing sugar content and 100-berry weight, decreased titratable acid content, and improved the maturity factor. Compared with the vines not sprayed with BLF, the content of total phenols and total anthocyanins in grape skins showed an increase over the three-year period, with the largest increases of 31.92% and 48.38%, respectively, and the content of total tannins and total flavan-3-ols increased in 2020 and 2021. BLF treatment also increased the total phenolic content in seeds for all three years, reaching a significant level in 2019, 16.38% higher than control treatment (CK). HPLC analysis showed that BLF treatment affected the content and composition of monomeric anthocyanins in grape skins, especially in 2021, BLF treatment significantly increased the content of nine monomeric anthocyanins, and the proportion of acetylated and coumaroylated anthocyanins. However, GC-MS analysis indicated that BLF had little effect on volatile compounds. These results suggest that BLF can be used as an overwintering protection measure in cold regions to promote the accumulation of sugars and polyphenolics, thereby improving overall grape quality.Keywords:wine grape;biodegradable liquid film;grape composition;anthocyanins
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BIOTECHNOLOGY LETTERS
·
2022
·Vol. 44
·期 5
·635-642
·
IF 2.716
Liu Jia,Liu Jianmin,Yang Bin,Gao Cong,Song Wei,Hu Guipeng,Liu Liming,Wu Jing
Objectives This study aimed to develop an efficient enzymatic strategy for the industrial production of phenylpyruvate (PPA) from l -phenylpyruvic acid ( l -Phe). Results l -amino acid deaminase from Proteus mirabilis was expressed in Escherichia coli BL21 (DE3) and modified to release product inhibition by employing conformational dynamics engineering. Based on structural analysis, two residues (E145/L341) were identified for reducing interactions between the product and enzyme and increasing flexibility of the protein, thereby facilitating the product release. The mutant M2 E145A/E341A exhibited a 3.84-fold reduction in product inhibition and a 1.35-fold increase in catalytic efficiency in comparison to the wild type. Finally, 81.2 g/L PPA production with a conversion of 99.6% was obtained in a 5-L bioreactor. Conclusions The engineered catalyst can significantly reduce product inhibition and facilitate the effective industrial synthesis of PPA.
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Microbial Cell Factories
·
2022
·Vol. 21
·期 1
·1-9
·
IF 6.352
Wang Yingying,Chen Xueman,Chen Qiaoyu,Zhou Ning,Wang Xin,Zhang Alei,Chen Kequan,Ouyang Pingkai
Background l-Tryptophan (l-Trp) derivatives such as 5-hydroxytryptophan (5-HTP) and 5-hydroxytryptamine (5-HT), N-Acetyl-5-hydroxytryptamine and melatonin are important molecules with pharmaceutical interest. Among, 5-HT is an inhibitory neurotransmitter with proven benefits for treating the symptoms of depression. At present, 5-HT depends on plant extraction and chemical synthesis, which limits its mass production and causes environmental problems. Therefore, it is necessary to develop an efficient, green and sustainable biosynthesis method to produce 5-HT.Results Here we propose a one-pot production of 5-HT from l-Trp via two enzyme cascades for the first time. First, a chassis cell that can convert l-Trp into 5-HTP was constructed by heterologous expression of tryptophan hydroxylase from Schistosoma mansoni (SmTPH) and an artificial endogenous tetrahydrobiopterin (BH4) module. Then, dopa decarboxylase from Harminia axyridis (HaDDC), which can specifically catalyse 5-HTP to 5-HT, was used for 5-HT production. The cell factory, E. coli BL21(DE3)△tnaA/BH4/HaDDC-SmTPH, which contains SmTPH and HaDDC, was constructed for 5-HT synthesis. The highest concentration of 5-HT reached 414.5 ± 1.6 mg/L (with conversion rate of 25.9 mol%) at the optimal conditions (substrate concentration,2 g/L; induced temperature, 25℃; IPTG concentration, 0.5 mM; catalysis temperature, 30℃; catalysis time, 72 h).Conclusion sThis protocol provided an efficient one-pot method for converting. l-Trp into 5-HT production, which opens up possibilities for the practical biosynthesis of natural 5-HT at an industrial scale.
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