INTERNATIONAL IMMUNOPHARMACOLOGY
·
2025
·
IF 4.7
You Zhou,You Wu,Rui Zhu
Background Septic acute kidney injury (S-AKI) is a major cause of acute kidney injury (AKI), but no effective therapies exist to prevent or treat it. Although KLF6 is linked to various pathophysiological processes, its exact role in S-AKI is not yet fully understood. Methods The role of KLF was investigated using both the mouse CLP model and in vitro systems. To identify the transcriptional targets regulated by KLF6, ChIP-qPCR and luciferase reporter assays were carried out. Biochemical and cellular techniques were employed to assess cellular injury and lipid peroxidation levels. The extent of ferroptosis was evaluated through transmission electron microscopy (TEM). Results KLF6 levels were significantly increased in a renal IRI model. Knockdown of KLF6 using AAV9 reduced kidney injury in both in vitro and in vivo models, while KLF6 overexpression aggravated injury. Overexpression of KLF6 promoted ferroptosis, whereas its knockdown reduced lipid peroxidation and ferroptosis. KLF6 regulates NCOA4 expression by binding to its promoter, controlling ferroptosis. Knockdown of NCOA4 in vitro alleviated ferroptosis and cellular damage caused by KLF6 overexpression. Pharmacological inhibition of KLF6 reduced kidney injury and ferroptosis in S-AKI mice. Conclusion Our study uncovered a previously unrecognized role for KLF6 in S-AKI. By facilitating NCOA4 transcription, KLF6 intensified ferroptosis, which, in turn, aggravated septic AKI.
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PubMed
高影响因子
NATURE
·
2025
·Vol. 637
·期 8047
·846-853
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IF 48.5
Song Huimin,Münch Konrad,Liu Xu,Shen Kaier,Zhang Ruizhuo,Weintraut Timo,Yusim Yuriy,Jiang Dequan,Hong Xufeng,Meng Jiashen,Liu Yatao,He Mengxue,Li Yitao,Henkel Philip,Brezesinski Torsten,Janek Jürgen,Pang Quanquan
With promises for high specific energy, high safety and low cost, the all-solid-state lithium–sulfur battery (ASSLSB) is ideal for next-generation energy storage 1 , 2 , 3 , 4 , 5 . However, the poor rate performance and short cycle life caused by the sluggish solid–solid sulfur redox reaction (SSSRR) at the three-phase boundaries remain to be solved. Here we demonstrate a fast SSSRR enabled by lithium thioborophosphate iodide (LBPSI) glass-phase solid electrolytes (GSEs). On the basis of the reversible redox between I − and I 2 /I 3 − , the solid electrolyte (SE)—as well as serving as a superionic conductor—functions as a surficial redox mediator that facilitates the sluggish reactions at the solid–solid two-phase boundaries, thereby substantially increasing the density of active sites. Through this mechanism, the ASSLSB exhibits ultrafast charging capability, showing a high specific capacity of 1,497 mAh g −1 sulfur on charging at 2C (30 °C), while still maintaining 784 mAh g −1 sulfur at 20C. Notably, a specific capacity of 432 mAh g −1 sulfur is achieved on charging at an extreme rate of 150C at 60 °C. Furthermore, the cell demonstrates superior cycling stability over 25,000 cycles with 80.2% capacity retention at 5C (25 °C). We expect that our work on redox-mediated SSSRR will pave the way for developing advanced ASSLSBs that are high energy and safe.
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高影响因子
Science China-Chemistry
·
2025
·
IF 9.7
Huang Wenhuan,Wang Shun,Hou Xuehan,Zhang Yanan,Zhang Xingxing,Zhao Yaoxiao,Jin Xilang,Zhang Huabin,Wang Fei,Zhang Jian
Lithium-sulfur (Li-S) battery, as a promising next-generation high-energy-density battery, suffers from lithium polysulfides (LPSs) shuttle and Li dendrites issues. Porous crystalline framework-based membranes as separator can effectively absorb and restrain LPSs but without precise structural design principal exploration and mechanism studies. Herein, bio-inspired 1D oriented lithium-ion transport channels with molecular negatively charged PO 3 (−1) have been developed in hybrid tetrazole frameworks for promoting the transmission of Li + ions. The theoretical calculations and in situ spectroscopy demonstrated the higher binding energy and inhibited diffusion of LPSs in PO 3 . The assembled PP@PO 3 Janus separator in Li-S coin cell delivered a high initial capacity of 1410.9 mAh g −1 at 0.1 C and a low attenuation rate of 0.038% per cycle over 500 cycles at 5 C. Besides, the high capacities of 862, 542 and 409 mAh g −1 based on high-sulfur-loading cathodes of 2.6, 3.8 and 5.0 mg cm −2 at 0.5 C were achieved, respectively. Moreover, the punch battery of PO 3 @PP separator with S-cathode of 1.2 mg cm −2 has been developed for demonstrating its potentials commercial application, which displays impressive capacity of 873.4 mAh g −1 with the retention of ∼78.9% over 50 cycles.
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MICROPOROUS AND MESOPOROUS MATERIALS
·
2024
·
IF 4.8
Ying Zhang,Qinqin Wang,Mingyuan Zhu,Bin Dai
The prevailing Zn/AC catalysts was plagued by limited lifetime and difficulties in regeneration for acetylene acetoxylation. In order to exploring renewable catalyst for acetylene acetoxylation, Zn/S-1 catalyst was synthesized using Silicalite-1(S-1) as support. The catalytic performance of Zn/S-1 was compared with Zn/AC catalyst using active carbon as support. The CH 3 COOH conversion of Zn/S-1 catalyst only decreased 15 % after running 225 h, much better than the 66 % of Zn/AC after 154 h. TG analysis revealed that the improved stability of Zn/S-1 catalyst was attributed to its better resistance for carbon deposition with the presence of S-1 support. A series of characterizations and DFT results elucidated that the Lewis acid sites of S-1 support and Zn component serve as dual active sites, which enhanced the adsorption of CH 3 COOH and inhibited the carbon deposition. In addition, the deactivated Zn/S-1 catalyst could be regenerated by calcination in air atmosphere due to the thermal stability of S-1, and the regenerated Zn/S-1 catalyst showed similar catalytic activity with the fresh catalyst.
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高影响因子
STAR Protocols
·
2024
·
IF 1.3
Chunyang Xie,Dong Luo,Lu Liu,Xue Huang,Shao-Lin Zhang,Aimin Yang
Summary S -acylation of proteins allows their association with membranes. Here, we present a protocol for establishing a platform for membrane affinity evaluation of S -acylated proteins in vitro . We describe steps for preparing lipid-maleimide compounds, mCherry-p62 recombinant proteins, and total cellular membranes. We then detail procedures for synthesizing protein-lipid conjugates using lipid-maleimide compounds and recombinant proteins and evaluating the membrane affinity of protein-lipid conjugates. For complete details on the use and execution of this protocol, please refer to Huang Xue et al. 1
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高影响因子
Joule
·
2023
·
IF 39.8
Yatao Liu,Linhan Xu,Yongquan Yu,MengXue He,Han Zhang,Yanqun Tang,Feng Xiong,Song Gao,Aijun Li,Jianhui Wang,Shenzhen Xu,Doron Aurbach,Ruqiang Zou,Quanquan Pang
The transition from dissolution-precipitation to quasi-solid-state sulfur reaction promises restricted polysulfide shuttle and lean electrolyte operation of Li-S batteries but incurs poor reaction kinetics. We here demonstrate that structural reorganization of sparingly solvating electrolytes (SSEs)—which is uniquely afforded by using low-density and low-cost aromatic anti-solvents—is vital for taming the quasi-solid-state sulfur reaction. Aromatic anti-solvents disrupt the interconnected structure of concentrated tetrahydrofuran (THF) electrolyte, uniquely creating subdomains that act to dissolve elemental sulfur, thus accelerating its consumption and re-formation while maintaining ultralow polysulfides solubility. The altered subdomains further result in robust solid electrolyte interphase (SEI) on lithium metal. As a result, the Li-S cell with a 3 mg sulfur cm −2 sulfur cathode can cycle steadily for ∼160 cycles with a lean electrolyte of 5 μL mg sulfur −1 . Our work provides new insights into fine-tuning the electrolyte microstructure through solvent innovations for developing sulfur-based batteries that are high energy, cheap, and durable.
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Ya-Nan Jing,Xing-Liang Yin,Lei-Lei Li,Yan-Lan Wang,Jia Xue,Ze-Feng Xu,Da-Qiang Liu,Chuan-Wu Chen,Xiao-Jie Liu,Er-Kang Liu
CO 2 -to-high value-added chemicals via a photocatalytic route is of interest but strangled by the low efficiency. Herein, a novel Fe-TiO 2-x /TiO 2 S-scheme homojunction was designed and constructed by using a facile surface modification approach whereby oxygen vacancy (OV) and Fe introducing on the TiO 2 nanorod surface. The as-synthesized Fe-TiO 2-x /TiO 2 S-scheme homojunction exhibits positive properties on promoting photocatalytic CO 2 reduction: i) the nanorod structure provides numerous active sites and a radical charge transfer path; ii) the doped Fe and OV not only synergistically enhance light utilization but also promote CO 2 adsorption; iii) the Fe-TiO 2-x /TiO 2 S-scheme homojunction benefits photoexcited charge separation and retains stronger redox capacity. Thanks to those good characters, the Fe-TiO 2-x /TiO 2 homojunction exhibits superior CO 2 reduction performances with optimized CO/CH 4 generation rates of 122/22 μmol g -1 h −1 which exceed those of pure TiO 2 by more than 9.4/7.3 folds and most currently reported catalytic systems. This manuscript develops a facile and universal approach to synthesize well-defined homojunction and may inspire the construction of other more high-efficiency photocatalysts toward CO 2 reduction and beyond.
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高影响因子
PLANT BIOTECHNOLOGY JOURNAL
·
2019
·Vol. 18
·期 7
·1550-1561
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IF 6.84
Qi Gao,Huifeng Luo,Yongping Li,Zhongchi Liu,Chunying Kang
Summary Fruit colour affects consumer preference and is an important trait for breeding in strawberry. Previously, we isolated the Reduced Anthocyanins in Petioles ( RAP ) gene encoding a glutathione S-transferase (GST) that binds anthocyanins to facilitate their transport from cytosol to vacuole in the diploid strawberry Fragaria vesca . The parent of rap was the F. vesca variety ‘Yellow Wonder’ that develops white fruit due to a natural mutation in the FveMYB10 gene. Here, we investigated the application potential of RAP in modulating fruit colours by overexpression of RAP in F. vesca and knockout of RAP in the cultivated strawberry Fragaria × ananassa . Unexpectedly, the RAP overexpression in Yellow Wonder background caused formation of red fruit. In addition, the red coloration occurs precociously at floral stage 10 and continues in the receptacle during early fruit development. Transcriptome analysis revealed that the anthocyanin biosynthesis genes were not up-regulated in RAP-ox; rap myb10 flowers at anthesis and largely inhibited at the turning stage in fruit, suggesting a coloration mechanism independent of FveMYB10 . Moreover, we used CRISPR/Cas9 to knockout RAP in cultivated strawberry which is octoploid. Six copies of RAP were simultaneously knocked out in the T0 generation leading to the green stem and white-fruited phenotype. Several T1 progeny have segregated away the CRISPR/Cas9 transgene but maintain the green stem trait. Our results indicate that enhancing the anthocyanin transport could redirect the metabolic flux from proanthocyanidin to anthocyanin production at early developmental stages of fruit and that RAP is one promising candidate gene in fruit colour breeding of strawberry.
研究领域:
植物生物学
·
遗传学
·
分子生物学
·
农学
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高影响因子
Nature Chemical Biology
·
2024
·
IF 12.9
Wang Shiwen,Liu Min,Hu Dongping,Dong Zhicheng,Zhao Zhong
Superoxide anion is thought to be a natural by-product with strong oxidizing ability in all living organisms and was recently found to accumulate in plant meristems to maintain stem cells in the shoot and undifferentiated meristematic cells in the root. Here we show that the DNA demethylase repressor of silencing 1 (ROS1) is one of the direct targets of superoxide in stem cells. The Fe–S clusters in ROS1 are oxidized by superoxide to activate its DNA glycosylase/lyase activity. We demonstrate that superoxide extensively participates in the establishment of active DNA demethylation in the Arabidopsis genome and that ARABIDOPSIS RESPONSE REGULATOR 12 acts downstream of ROS1-mediated superoxide signaling to maintain stem cell fate. Our results provide a mechanistic framework for superoxide control of the stem cell niche and demonstrate how redox and DNA demethylation interact to define stem cell fate in plants.
研究领域:
植物生物学
·
表观遗传学
·
干细胞生物学
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高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2021
·
IF 9.028
Yang Yang,Rui Huang,Wenqing Xu,Jixiang Zhang,Chaoqun Li,Jianfei Song,Tingyu Zhu
ZnS is a promising sorbent in recovering Hg0 from industrial flue gas due to its excellent Hg0 adsorption capacity. However, the internal structure–activity relationship still needs to be further clarified. In this work, ZnS sorbents with different structures were synthesized with the hydrothermal method by tuning the temperature. The samples had significant differences in the crystallinity, morphology, particle size, and sulfur (S) active sites. The results indicated that Hg0 removal performance was determined by the specific surface area and S active sites. ZnS synthesized at low temperatures (80-ZnS and 120-ZnS) had a larger surface area, while the S sites on the high-temperature-synthesized sample (160-ZnS) were more active for Hg0 adsorption. The 160-ZnS sample exhibited a much higher Hg0 adsorption amount per unit surface area. Further characterization revealed that S22– and Sx were the main active sites for Hg0 adsorption. Sx existed in the form of long-chain polysulfur (L-Sx) on 80-ZnS and 120-ZnS, while it exhibited in the form of short-chain polysulfur (S-Sx) on 160-ZnS. L-Sx had negligible adsorption ability, while S-Sx had a high affinity for Hg0. Hg0 can react with S22– and S-Sx, forming α-HgS and β-HgS, respectively. The new insight in this work can provide theoretical guidance for the design and structure optimization of ZnS, facilitating its practical industrial application.
研究领域:
环境科学
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材料科学
·
化学工程
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高影响因子
CANADIAN JOURNAL OF PLANT SCIENCE
·
2015
·Vol. 95
·期 5
·
IF 0.919
ZhaoLi-Xia,QuHai-Tao,FuYing,GaoShuang,YeFei
Zhao, L.-X., Qu, H.-T., Fu, Y., Gao, S. and Ye, F. 2015. Alleviation of injury from chlorimuron-ethyl in maize treated with safener 3-dichloroacetyl oxazolidine. Can. J. Plant Sci. 95: 897–903. The protective effects of herbicide safeners, including 3-dichloroacetyl-2,2-dimethyl-1,3-oxazolidine (R-28725), 3-dichloroacetyl-2,2-dimethyl-4-ethyl-1,3-oxazolidine (Racemate), and its two enantiomers (R)-3-dichloroacetyl-2,2-dimethyl-4-ethyl-1,3-oxazolidine (R-enantiomer) and (S)-3-dichloroacetyl-2,2-dimethyl-4-ethyl-1,3-oxazolidine (S-enantiomer), on reducing the phytotoxicity of chlorimuron-ethyl to maize were investigated. Soaking the seeds in safeners increased the endogenous glutathione (GSH) content and glutathione-S-transferase (GST) activity of maize. When induced by R-enantiomer, the GST activity in vivo and in vitro increased 180 and 192% compared with control, respectively. R-28725 and R-enantiomer also increased the acetolactate synthase (ALS) activity inhibited by chlorimuron-ethyl from 45 to 100 and 97% compared with the control, respectively. The kinetic parameter Vmax of GST in the maize treated with R-28725 and R-enantiomer increased by 103 and 92%, respectively, compared with the control. Our results suggest that R-28725 and R-enantiomer could significantly improve the GSH content, GST activity, and ALS activity of maize. Overall, maize could be protected from the injury caused by chlorimuron-ethyl.
研究领域:
植物科学
·
农业化学
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2022
·
IF 5.999
Xiaomei Dai,Xiaojun Liu,Lele Yang,Siyuan Yuan,Qingqing Xu,Yu Li,Feng Gao
Intracellular bacterial infections pose enormous challenges to food safety and public health. Antibiotic-based polymer prodrugs have been used to treat intracellular bacterial infection. However, the overuse of antibiotics may lead to the emergence of antibiotic resistance . In this work, we aimed to develop antibiotic-free pH-responsive polymeric prodrugs to combat intracellular S. aureus infection. Amphiphilic poly(ethylene glycol)- b -poly[(3-phenylprop-2-ene-1,1-diyl)bis(oxy)bis(enthane-2,1- diyl)diacrylate] (PEG- b -PCAE) was obtained by radical polymerization and they could self-assemble to form micelles . PEG- b -PCAE micelles could uptake by macrophage. Upon exposure to the acidic phagolysosome, PEG- b -PCAE micelles could release cinnamaldehyde (CA) through hydrolysis of the acetal linkage. PEG- b -PCAE could kill intracellular bacteria by damaging the bacterial membrane. Furthermore, PEG- b -PCAE micelles could generate reactive oxygen species (ROS) in macrophages and subsequently activate immune system to clear bacteria by inducing macrophages differentiation to M1 phenotype. PEG- b -PCAE micelles could accelerate the wound healing process of the S. aureus -infected model in vivo . It is anticipated that multifunctional antibiotic-free PEG- b -PCAE micelles with intrinsic antibacterial activities hold promise for improved outcomes in intracellular S. aureus infections.
研究领域:
微生物学
·
免疫学
·
药物化学
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