POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2025
·
IF 6.8
Lijun Ling,Rongxiu Mo,Wenyue Zhang,Yijuan Jiang,Fanjin Kong,Lijun Feng,Yao Li,Rui Yue,Yongpeng Zhou
The antifungal activity of six microbial-derived volatile organic compounds (VOCs) [ 3-hepten-2-one (Hep), cis-3-octen-1-ol, 3-methylvalerate, isovalerate, 1-octen-3-ol (Oct), and 2-methylbutyrate] against Aspergillus flavus was investigated by determining the minimum inhibitory concentration (MIC) and the fractional inhibitory concentration index (FICI). The results demonstrated that all 15 binary combinations of the VOCs presented additive effects against A. flavus . Among them, the combination of Hep and Oct, refers to C OH , with concentrations of 2 μL L −1 and 2.5 μL L −1 , respectively, displayed the most potent antifungal effect with the lowest concentration, in contrast to the individual MIC of Hep or Oct, which were reduced to 1/8 and 1/2. C OH can induce membrane lipid peroxidation, enhance cell membrane permeability, and cause leakage of nucleic acids and proteins within the cell, and 1-octen-3-ol might play a leading role. Finally, applying C OH at a concentration of 1/2 MIC Oct + 1/4 MIC Hep could effectively restrain the spread of A. flavus on wolfberries and exhibit excellent preservation effects. This study validates the antifungal effect of C OH and may serve as a natural alternative to antimicrobial agents for post-harvest agricultural products.
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高影响因子
MICROCHEMICAL JOURNAL
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2021
·
IF 4.821
Jian-Bo Chen,Xueyan Zhao,Zhenqi Zhang,Ya Chen,Xiaolin Fu,Yu Liu
Recently, 3,3-diamino-4,4-azoxyfurazan (DAAF) as one of most promising energetic furazan compounds has been widely studied because of its excellent explosive performances. Nowadays, the Oxone oxidation method is regarded as a main pathway to synthesize DAAF. However, several unknown impurities are yielded when the Oxone method is used to prepare DAAF. The identification of DAAF’s impurities is beneficial to optimize synthetic route of DAAF and then improve its purity. In this study, chemical structures of DAAF and its main impurities were analyzed by developing an unknown analysis workflow based on ultrahigh-performance liquid chromatography-tandem diode array detector -tandem high resolution mass spectrometry (UHPLC-DAD-HRMS). They were fully separated by UHPLC after the optimization of chromatographic conditions. HRMS based on a Q-Orbitrap system was performed to elucidate chemical structure of the impurities by determining exact mass of the impurities and their MS/MS fragments. Besides DAAF, eight unknown impurities were detected under negative ion mode ([M-H] - ) of HRMS with a heated electrospray ionization. Comprehensive analysis of UHPLC-DAD-HRMS provided more information including the retention time, UV–Vis spectrum, elementary composition and MS/MS fragments, and then possible structures of the impurities were proposed in this work. Further, the ion at m / z 211.0328, impurity I and impurity V were confirmed to be DAAF, DAF and DAAzF, respectively, through 1 H NMR and 13 C NMR after synthesizing their reference materials. In addition, the source of these impurities during the synthesis of DAAF has been analyzed. The impurities were originated from the precursor, the intermediate and byproduct of preparing DAAF through the hydrazine, the isomerism and the nitrosation.
研究领域:
化学
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材料科学
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高影响因子
GREEN CHEMISTRY
·
2015
·Vol. 17
·期 7
·3992-3999
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IF 8.02
Shujing Zhu,Xiaoqiang Ma,Erzheng Su,Dongzhi Wei
From an environmental perspective, utilizing nonconventional solvents (i.e. green solvents) with low ecological footprints is a highly beneficial alternative to using conventional organic solvents to form a reaction system. The nitrile hydratase (NHase, EC 4.2.1.84) catalyzed hydration of 2-amino-2,3-dimethylbutyronitrile (ADBN) to 2-amino-2,3-dimethylbutyramide (ADBA) in various green solvent–aqueous reaction systems was investigated in this study. After systematically optimizing the reaction conditions, the HFE-7100/H2O (v/v, 10%) biphasic system was ultimately identified as a promising reaction system for reducing product inhibition, avoiding substrate hydrolysis, and facilitating product separation and solvent recovery. The average ADBA yield of an entire batch reaction was 97.3%, which is obviously higher than those obtained with previously reported chemical or enzymatic methods. This is the first attempt to apply a fluorous solvent–aqueous biphasic system to a biocatalytic process, and the results suggest that the fluorous solvent employed in the biphasic system satisfies the requirements for green chemistry.
研究领域:
生物化学
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环境科学
·
绿色化学
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高影响因子
ACS Applied Materials & Interfaces
·
2026
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IF 7.8
Xiaopeng Zhang,Lin Chen,Longzhu Li,Xiaoming Yan,Junjiang Bao,Ning Zhang,Gaohong He
The introduction of guest metal molecules into metal–organic frameworks (MOFs) is an outstanding method to prepare dual-atom catalysts (DACs) with good oxygen reduction reaction (ORR) activity. However, most of the MOF-derived dual-atom catalysts reported to date exhibit a microporous structure and, therefore, suffer from the underutilization of active sites and poor mass transfer. This study employed NH2-MIL-101 as a precursor to form defect-bearing mesoporous Fe-NC via one-step pyrolysis for adsorbing [Co(en)3]3+, followed by secondary pyrolysis to generate Fe–Co binuclear site catalysts. The mesopores of NH2-MIL-101 are beneficial to [Co(en)3]3+ introduction and can improve the mass transfer of the ORR. Furthermore, the elevated nitrogen content within the [Co(en)3]3+ complex facilitates the co-coordination of Fe and Co with nitrogen, thereby enhancing the formation of Fe–Co diatomic sites. As a result, the obtained FeCo-NC-40 catalyst showed excellent ORR performance with a half-wave potential of 0.915 V (vs RHE). In addition, the liquid-phase Zn–air battery employing FeCo-NC-40 as the cathode catalyst demonstrated a peak power density of 178.13 mW·cm–2 and achieved a discharge specific capacity of 806.13 mAh·g–1 at 10 mA·cm–2, and there was no significant decay in stability for 10 days of continuous cycling.
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PubMed
高影响因子
JOURNAL OF APPLIED MICROBIOLOGY
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2025
·Vol. 136
·期 11
·
IF 3.2
Ling Lijun,Zhang Wenyue,Mo Rongxiu,Kong Fanjin,Jiang Yijuan,Feng Lijun,Li Yao,Yue Rui,Zhou Yongpeng
AimsLanzhou lily (Lilium davidli var. unicolor) possesses both nutritional and medicinal value, however, its bulbs are highly susceptible to postharvest physical damage. Pathogenic bacteria readily infiltrate through these wounds, resulting in extensive decay and consequent economic losses. This study demonstrates that optimized volatile organic compound (VOC) combinations can significantly reduce effective antimicrobial concentrations and broaden the antimicrobial spectrum through synergistic effects, while exhibiting excellent preservation potential.Methods and ResultsFrom decayed lily bulbs, this study isolated and identified two bacterial strains, Bacillus cabrialesii SH-3 and Bacillus amyloliquefaciens SH-5, both exhibiting opportunistic pathogenicity. Fifteen binary VOC combinations were evaluated using fractional inhibitory concentration index (FICI), identifying QM (1/8 MIC 2-methylbutyric acid + 1/8 MIC 3- methylpentanoic acid, FICI = 0.25) and QH (1/2 3-methylpentanoic acid + 1/8 3-hepten-2-one, FICI = 0.625) as optimal formulations. Mechanistic studies revealed these combinations disrupt bacterial membrane integrity, induce protein/nucleic acid leakage, and trigger oxidative stress and metabolic dysfunction.ConclusionsAgainst SH-3, only QM showed synergistic effects, reducing required concentrations by 87.5% compared to individual minimum inhibitory concentration (MIC). For SH-5, all combinations demonstrated additive effects, with QH achieving potent inhibition at 50% and 87.5% reduced concentrations for respective components. Applied at MIC levels, both formulations significantly suppressed postharvest decay while maintaining bulb quality.
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2026
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IF 6.7
Jiaqi Fu,Lianghua Xie,Zhen Zong,Khairiah M. Alwutayd,Hany A. El-Shemy,Wei Chen
Abstract Cyanidin-3-O-rhamnoside (C3R) and cyanidin-3-O-glucoside (C3G) show strong anti-inflammatory and antioxidant activities with great potential for chronic disease prevention. However, C3R biosynthesis from (+)-catechin has not been reported, and C3G production from this substrate is low for industrial applications. Here, we constructed a microbial platform for the efficient C3R and C3G production via genetic engineering and metabolic optimization. We screened glucosyltransferases and rhamnosyltransferases from various plants to identify the optimal combinations for C3G and C3R biosynthesis. Tuning UDP-glucose and UDP-rhamnose balance by plasmid copy number increased the C3R concentration by 120%. Overexpression of MdtH and yadH improved extracellular C3R and C3G accumulation by 16% and 31%, respectively. After optimizing substrate and culture conditions, the engineered strains produced 529.01 mg/L of C3R and 926.47 mg/L of C3G, with C3R synthesis reported for the first time. This study underscores the key roles of glycosyltransferases and UDP-sugar modulation, providing a versatile strategy for producing other disaccharide- or rhamnose-containing natural products.
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高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2021
·
IF 2.694
Huan Ye,Ying Yu,Sile Shi,Chi Cai,Haoran Liu,Weiping Luo
In this study, the solid–liquid equilibrium (SLE) data of 3-methyl-2-nitrobenzoic acid (3-M-2-NBA), 3-methyl-4-nitrobenzoic acid (3-M-4-NBA), and 5-methyl-2-nitrobenzoic acid (5-M-2-NBA) in n-butanol, iso-butanol, tert-butanol, and sec-butanol were determined using the method of dynamic laser at atmospheric pressure. The experimental SLE data indicate that within the temperature range studied (290.15–348.95 K), the solubilities of 3-M-2-NBA, 3-M-4-NBA, and 5-M-2-NBA in the above four solvents are proportional to the temperature. The molar fraction of solubility of the same solute in different solvents from low to high is as follows: iso-butanol < n-butanol< sec-butanol < tert-butanol. The molar fraction of solubility of the three solutes in the same solvent from low to high is as follows: 3-M-4-NBA < 3-M-2-NBA<5-M-2-NBA. The experimental data were correlated using the Apelblat model, λh model, and NRTL activity coefficient model, and the calculated solubilities agree satisfactorily with the measured results. The best model for every system was selected using the result of Akaike information criterion analysis.
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高影响因子
MOLECULES
·
2023
·Vol. 28
·期 8
·3616
·
IF 4.6
Liqun Zhang,Pinggu Wu,Xiaoling Xiang,Dajin Yang,Liyuan Wang,Zhengyan Hu
Refined and deodorized camellia oil has been reported to contain a high amount of 3-monochloropropane-1,2-diol esters (3-MCPDE) due to the high-temperature deodorization step. To reduce 3-MCPDE in camellia oil, the physical refining process of camellia oil was simulated on a laboratory scale. Response surface methodology (RSM) was designed to modify and optimize the refining process with five processing parameters (water degumming dosage, degumming temperature, activated clay dosage, deodorization temperature and deodorization time). The optimized new refining approach achieved a 76.9% reduction in 3-MCPDE contents, in which the degumming moisture was 2.97%, the degumming temperature was 50.5 °C, the activated clay dosage was 2.69%, the deodorizing temperature was 230 °C, and the deodorizing time was 90 min. A significance test and analysis of variance results demonstrated that the deodorization temperature and deodorization time contributed significantly to the reduction of 3-MCPD ester. The joint interaction effects of activated clay dosage and deodorization temperature were significant for 3-MCPD ester formation.Keywords:3-MCPD esters;response surface analysis;optimized refining process parameters;reduction of 3-MCPD
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高影响因子
Polymer Chemistry
·
2016
·Vol. 7
·期 39
·6120-6132
·
IF 5.687
Cai Wang,Yudong Zheng,Yi Sun,Jinsheng Fan,Qiujing Qin,Zhenjiang Zhao
A novel block polyurethane was designed and synthesized based on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), 4,4′-diphenylmethanediisocyanate (MDI) and poly(ethylene glycol) (PEG). The obtained polyurethanes were characterized by proton nuclear magnetic resonance (1H NMR), Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), differential scanning calorimetry (DSC) and wide angle X-ray diffraction (WAXD). Furthermore, the biodegradability of the synthesized polyurethanes was evaluated at 37 °C in phosphate buffer solution (PBS) at pH 7.4. The results showed that these PHBV-based polyurethanes were degradable in PBS, as indicated by weight loss measurements, scanning electron microscopy (SEM) and FTIR. The cytotoxicity of the novel polyurethane was evaluated using the cell counting kit-8 (CCK8) assay in vitro. Preliminary evaluation of hemocompatibility was carried out via the hemolysis test, dynamic blood coagulation time and platelet adhesion. The current work provides a promising approach to prepare nontoxic and biodegradable polyurethanes with excellent mechanical properties and good hemocompatibility. This new material may find potential application in blood vessel tissue engineering.
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高影响因子
ORGANIC & BIOMOLECULAR CHEMISTRY
·
2014
·Vol. 12
·期 46
·9366-9374
·
IF 3.487
Ting-Ting Feng,Xuan Huang,Xiong-Li Liu,De-Hong Jing,Xiong-Wei Liu,Feng-Ming Guo,Ying Zhou,Wei-Cheng Yuan
The first phase transfer-catalysed direct γ-substitution of Morita–Baylis–Hillman carbonates of isatins with 3-substituted oxindoles has been developed, which affords 3-alkenyl-oxindole ring-fused 3,3′-disubstituted oxindoles in up to 83% yield under mild reaction conditions. Furthermore, their biological activity has been preliminarily demonstrated by in vitro evaluation against human prostate cancer cells PC-3 and human leukemia cells K562, using MTT-based assays with the commercially available standard drug Cisplatin as a positive control. Gratifyingly, compounds 3aa, 3ba and 3ca exhibited comparable in vitro inhibitory activities against human prostate cancer cells (PC-3) to Cisplatin. What's more, 3ba also had a good inhibition ability against human leukemia cells K562. These results indicate that 3-alkenyl-oxindole ring-fused 3,3′-disubstituted oxindole analogs may be potential lead compounds for further biological screening.
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PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2013
·
IF 3.777
Xuemin Chen,Tongqing Ren,Ming Ma,Zhengguo Wang,Guoqing Zhan,Chunya Li
Using carboxylic acid-functionalized single walled carbon nanotubes (SWCNTs-COO − ) as an anion and 3-butyl-1-[3-( N -pyrrolyl)propyl]imidazolium as a cation, a novel SWCNTs-COO-ionic liquid (SWCNTs-COO-IL) nanocomposite was fabricated successfully. The as-prepared SWCNTs-COO-IL nanocomposite was confirmed with transmission electron microscopy, X-ray photoelectron spectroscopy, UV–vis, FTIR and Raman spectroscopy. The SWCNTs-COO-IL nanocomposite was coated onto a glassy carbon electrode surface followed by cyclic voltammetric scanning to fabricate a SWCNTs/poly{3-butyl-1-[3-( N -pyrrolyl)propyl] imidazolium ionic liquid} composite film modified electrode (SWCNTs/Poly-IL/GCE). Scanning electron microscope and electrochemical impedance spectroscopy were used to characterize SWCNTs/Poly-IL/GCE. Electrochemical behaviors of bisphenol A (BPA) at the SWCNTs/Poly-IL/GCE were investigated thoroughly. It was found that an obvious oxidation peak appeared without reduction peak in the reverse scanning, indicating an irreversible electrochemical process. The oxidation peak currents of BPA were linearly related to scan rate in the range of 20–300 mV s −1 , suggesting an adsorption controlled process rather than a diffusion controlled process. Differential pulse voltammetry was employed for the voltammetric sensing of BPA. Experimental conditions such as film thickness, pH value, accumulation potential and time that influence the analytical performance of the SWCNTs/Poly-IL/GCE were optimized. Under optimal conditions, the oxidation peak current was linearly related to BPA concentration in the range of 5.0 × 10 −9 to 3.0 × 10 −5 mol L −1 with a detection limit of 1.0 × 10 −9 mol L −1 ( S / N = 3). The developed method was successfully employed for the determination of BPA leaching from plastic drinking bottle.
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Current Protocols
·
2023
·Vol. 3
·期 5
·e741
·
IF 0
Wen-Wu Sun,Yi-Bing Xie,Ting-Ting Deng,Jie Huang,Ji-Kai Liu,Bin Wu
Despite the importance of nucleosides and nucleotides for drug discovery, only a few practical methods to prepare tricyclic nucleosides have been reported. Here, we describe a synthetic strategy for late-stage functionalization of nucleosides and nucleotides via chemo- and site-selective acid-promoted intermolecular cyclization. The nucleoside analogs with an additional ring were obtained in moderate-to-high yields, including some antiviral drugs (acyclovir, ganciclovir, and penciclovir) derivatives, endogenous fused ring nucleoside (M 1 dG) and its derivatives, and nucleotide derivatives. © 2023 Wiley Periodicals LLC. Basic Protocol 1 : Synthesis of tricyclic acyclovir analogs ( 3a-3c ) Basic Protocol 2 : Synthesis of tricyclic nucleosides M 1 dG ( 6 ) and M 1 G ( 9 ) Basic Protocol 3 : Synthesis of tricyclic nucleotide ( 12 )
研究领域:
药物化学
·
有机合成
·
核酸化学
·
分子生物学
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化学生物学
·
抗病毒药物研发
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危险品化学品经营许可证（带存储）