Analytical Methods
·
2021
·Vol. 13
·期 20
·2305-2312
·
IF 2.896
Quanxiu Zhou,Zhaofa Li,Qunfang Wang,Liang Peng,Shihe Luo,Feng Long Gu
In this work, poly(styrene-co-maleic anhydride)-capped CdSe/ZnS quantum dots (QDs) aminolyzed with ethanolamine are proposed as fluorescent probes for the detection of Cu2+ and Hg2+, and two different quenching mechanisms are discussed in detail. The coordination abilities of the surface polymer of CdSe/ZnS QDs and two metal ions are calculated by density functional theory (DFT). The photoinduced electron transfer from excited QDs to Cu2+ unoccupied orbitals is enhanced due to the coordination between Cu2+ and the surface polymer of QDs. The electron transfer consumes non-radiative energy and performs fluorescence quenching. For Hg2+, the formation of HgS and the slight aggregation of polymer-coated CdSe/ZnS QDs lead to fluorescence quenching. The probe is sensitive to both Cu2+ and Hg2+, and the response can be detected within 1 min without adjusting the pH. With the addition of a masking agent, Cu2+ and Hg2+ can be exclusively detected in coexistence with another ion. For Cu2+, a linear relation in the concentration ranging from 0.02 to 0.7 μM was found between the relative fluorescence intensity (F0/F) and the concentration of Cu2+; the limit of detection (S/N = 3) is 6.94 nM. For Hg2+, a linear relation ranging from 0.1 to 1.4 μM was found between ln(F0/F) and the concentration of Hg2+; the limit of detection is 20.58 nM.
研究领域:
分析化学
·
材料科学
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JOURNAL OF FLUORESCENCE
·
2019
·Vol. 29
·期 3
·727-735
·
IF 1.913
Huang Kun,Han Defang,Li Xianglin,Peng Mengni,Qiu Qi,Qin Dabin
A new biscarbazole-fused xanthene hybrid dye MeBCX has been prepared by CH 3 SO 3 H-promoted condensation of 4-hydroxycarbazole and o -phthalic anhydride, followed by esterification with methanol, which exhibits good cell membrane permeability. Taking advantage of the spiro-controlled xanthene platform of the dye, a Cu 2+ -selective fluorescence “off-on” probe BCX-Cu has been successfully prepared with excellent performances in selectivity and sensitivity, and the detection limit is calculated to be 88.7 nM. The sensing mechanism is confirmed to involve a specific cascade reactions of Cu 2+ -induced spirolactam ring-opening and hydrolysis. In addition, living L929 cell imaging demonstrates the probe has living cell membrane permeability and shows great potential for tracing intracellular Cu 2+ through fluorescence imaging technology.
研究领域:
分析化学
·
生物医学工程
·
细胞生物学
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ACS Nano
·
2019
·
IF 13.903
Qian Huang,Shaohua Zhang,Hao Zhang,Yaobao Han,Hanghang Liu,Feng Ren,Qiao Sun,Zhen Li,Mingyuan Gao
The small difference between tumor and normal tissues in their responses to ionizing radiation has been a significant issue for radiotherapy of tumors. Herein, we report that dumbbell-shaped heterogeneous copper selenide-gold nanocrystals can serve as an efficient radiosensitizer for enhanced radiotherapy. The mean lethal dose of X-rays to 4T1 tumor cells can be drastically decreased about 40%, that is, decreasing from 1.81 to 1.10 Gy after culture with heterostructures. Due to the synergetic effect of heterostructures, the dose of X-rays is also much lower than those obtained from mixture of Cu2–xSe + Au nanoparticles (1.78 Gy), Cu2–xSe nanoparticles (1.72 Gy) and Au nanoparticles (1.50 Gy), respectively. We demonstrate that the sensitivity enhancement ratio of Cu2–xSe nanoparticles was significantly improved 45% (i.e., from 1.1 to 1.6) after the formation of heterostructures with gold. We also show that the heteronanocrystals exhibit an enhanced photothermal conversion efficiency, due to the synergetic interactions of localized surface plasmon resonance. These properties highly feature them as a multimodal imaging contrast agent (particularly for photoacoustic imaging, computed tomography imaging, and single photon emission computed tomography after labeled with radioisotopes) and as a radiosensitizer for imaging guided synergetic radiophotothermal treatment of cancer. The research provides insights for engineering low-Z nanomaterials with high-Z elements to form heteronanostructures with enhanced synergetic performance for tumor theranostics.
研究领域:
生物医学工程
·
癌症治疗
·
纳米医学
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高影响因子
Journal of Water Process Engineering
·
2024
·
IF 6.3
Can Cai,Longfei Gao,Yuzhu Xiong
In this work, Fe 3 O 4 magnetic particles were in-situ grown on the surface of Montmorillonite (MTT) via co-precipitation method, and a green, recyclable chitosan (CS)/carboxymethyl cellulose (CMC)/sodium alginate (SA)/MMT-Fe 3 O 4 double network (DN) hydrogel (C-M-SD/MMT-Fe 3 O 4 ) was successfully synthesized. The magnetization of MMT-Fe 3 O 4 addition improved the maximum compressive strength of CS/CMC/SA double network hydrogel (C-M-SD), reaching 17.13 kPa. The adsorption capacities of the magnetized DN hydrogel for methylene blue (MB) and Cu 2+ were 224.49 mg/g and 121.15 mg/g, respectively. Moreover, cytotoxicity tests using CCK8 solution showed that the cell activity was >80 % after 24 h culture in the prepared hydrogel extracts, which demonstrated that the prepared hydrogel adsorbent did not release any toxic substances into the water source throughout the adsorption process . It can be concluded from the Vibrating Sample Magnetometer (VSM) that the saturation magnetization of the prepared magnetic double-network hydrogel reaches 15 emu/g and the coercivity is 0, showing the superparamagnetism . The synthesized C-M-SD/MMT-Fe 3 O 4 hydrogel possesses excellent mechanical properties, natural degradability, recyclability and biocompatibility, making it an ideal candidate for wastewater treatment applications.
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2026
·
IF 5.9
Huaqiang Luo,Bingjie Sun,Guitao Tan,Bingtian Tu,Jing Tu
Core-shell-shell PPH nanospheres were designed for antibiotic-free wound therapy. • Cationic PHMB and released Cu 2+ cooperatively eliminate bacteria and biofilms. • PDA layer regulates pH-responsive Cu 2+ release with favorable hemocompatibility. • PPH promotes HUVEC migration in vitro and neovascularization in vivo . • Accelerated wound remodeling was achieved with enhanced collagen deposition. Bacterial infection and biofilm formation pose major obstacles to wound healing. To address these challenges, we designed a core-shell nanocomposite, Cu-PBG@PDA@PHMB (PPH), consisting of a copper-doped phosphate glass (Cu-PBG) core, a polydopamine (PDA) interlayer, and a polyhexamethylene biguanide (PHMB) outer shell. The PPH nanospheres exert a complementary antibacterial effect via contact-killing and ion release: the cationic PHMB shell disrupts bacterial membranes through electrostatic interaction, whereas the PDA layer mediates pH-responsive Cu 2+ release. This combined effect is primarily driven by substantial contact-induced membrane damage, and the presence of released Cu 2 ⁺ ions further enhances this membrane-disruptive action, ultimately leading to efficient bacterial killing. In vitro evaluations demonstrated that PPH exhibited notable antibacterial activity against Staphylococcus aureus ( S. aureus ) and Escherichia coli ( E. coli ), markedly reduced mature biofilm biomass, and substantially disrupted biofilm integrity. Furthermore, the nanocomposite exhibited favorable biocompatibility and significantly promoted endothelial cell migration. In vivo experiments in a S. aureus -infected murine wound model demonstrated that PPH treatment was associated with accelerated wound closure, reduced bacterial burden, and improved angiogenesis and collagen deposition. This nanoplatform represents a promising therapeutic strategy for the clinical management of infected wounds.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Zinuo An,Liangbin Hu,Hongbo Li,Wei Zhou,Zhen Wang,Haizhen Mo,Zhenbin Liu,Xiaohui Zhou
Hyaluronic acid (HA) is widely used as a soft-tissue biomaterial due to its excellent biocompatibility and extracellular-matrix-like properties, yet its clinical utility is often limited by rapid biodegradation and bacteria-associated complication risk during subcutaneous procedures. Here, we developed an injectable copper-functionalized HA hydrogel (HACu-M) using a synergistic metal ion–carbodiimide crosslinking strategy that combines EDC/NHS-mediated covalent network formation with Cu 2+ coordination. HACu-M exhibited stable gelation, injectability, and self-healing behavior, together with improved mechanical robustness and physiological stability. In antibacterial assays, native HA promoted bacterial proliferation, whereas HACu-M reduced Staphylococcus aureus and Escherichia coli survival to <0.2% within 9 h and retained activity after long-term storage. HACu-M showed excellent hemocompatibility and cytocompatibility, supporting proliferation and migration of human skin fibroblasts and endothelial cells without inducing apoptosis. In a rat subcutaneous implantation model, HACu-M mitigated bacteria-associated tissue damage under S. aureus challenge, reduced inflammatory responses, and demonstrated prolonged in vivo retention compared with a commercial HA filler. Overall, HACu-M provides a multifunctional platform that integrates long-term stability with intrinsic antibacterial protection, highlighting its translational potential as a next-generation injectable filler for soft-tissue augmentation.
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ACS Applied Nano Materials
·
2026
·Vol. 9
·期 31
·14949-14963
·
IF 5.8
Yu Guangli,Sun Di,Ni Fei,Chang Meiji,Song Xingfei,Liang Yuan,Zhu Zhiqi,Zhao Rui,Wang Kangjun
The maintenance of human physiological homeostasis is
crucial for
public health, as the abnormal accumulation of Cu 2+ ions
in the body can lead to severe physiological damage. However, achieving
both convenient, reliable detection and efficient removal of excess
Cu 2+ ions in vivo remains a challenge. Herein, a series
of PAR@HP-NU-66 nanocomposites are synthesized by postgrafting 4-(2-pyridylazo)resorcinol
(PAR) onto HP-NU-66 frameworks for selective Cu 2+ ion detection
and elimination. As a tridentate chromogenic ligand, PAR forms stable
Cu 2+ chelates via metal-to-ligand charge transfer, inducing
a spectral red shift and a visible color change. This property enables
PAR@HP-NU-66 nanocomposite to colorimetrically detect Cu 2+ ions at a low limit of 0.25 mg L –1 in aqueous
solutions, which is below normal physiological levels. The nanocomposite
exhibits outstanding anti-interference performance against high-concentration
coexisting metal cations, amino acids, proteins and other endogenous
biomolecules; even under excess physiological interferents, its colorimetric
signal retention exceeds 90%. Moreover, a smartphone-assisted colorimetric
platform was developed to facilitate the rapid, on-site, and quantitative
detection of Cu 2+ ions. Meanwhile, the nanocomposite achieved
a superior adsorption capacity of up to 577.6 mg of Cu 2+ per gram of PAR@HP-NU-66, outperforming most previously reported
metal–organic framework (MOF)-based adsorbents. In vivo murine
therapeutic assays on copper-overload models confirm that PAR@HP-NU-66
reduces serum Cu 2+ levels by 41% with minimal histopathological
effects on tissues and organs. Density functional theory (DFT) calculations
confirm that the functionalization of HP-NU-66 with PAR introduces
electron-rich regions and dual synergistic chelation sites, thereby
boosting the adsorption capacity and eliciting a distinct colorimetric
response. These results highlight a promising dual-functional material
for Cu 2+ ion detection and removal, with great potential
for clinical diagnosis and treatment of Cu 2+ overload diseases.
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Yue Zhao,Yuqi Wu,Xinyuan Xue,Fanding Wang,Junli Shi,Mingya Wang,Jianlan Cui,Linxiu Zhao
A novel Schiff base fluorescent probe J-Y with a large Stokes shift (158 nm) and visible-region emission (575 nm) was developed. • J-Y forms a 1:1 complex with Cu 2+ through multiple chelation sites, enabling efficient fluorescence quenching via a PET mechanism. • Biothiols (Cys, GSH, Hcy) restore fluorescence by synergistic reduction of Cu 2+ and competitive coordination, achieving a highly sensitive “turn-on” response. • Dual-channel detection (UV–vis and fluorescence) with fluorescence LODs as low as 0.018 μM was demonstrated. • Practical applicability was validated through test strip assays, real food sample analysis and live-cell imaging. Biothiols play crucial roles in numerous physiological processes, making the development of novel fluorescent sensors for their detection highly valuable. Herein, a new fluorescent probe, J-Y, was designed, synthesized, and structurally characterized. The probe exhibits emission in the visible region (575 nm), a large Stokes shift (158 nm), and a wide applicable pH range. The probe J-Y can form a stable complex with Cu 2+ , and the competitive coordination ability of biothiols toward Cu 2+ lead to fluorescence recovery, enabling dual-channel UV–vis and fluorescence detection of biothiols (GSH, Cys, and Hcy). In a CH 3 CN/H 2 O system, the [ J-Y -Cu 2+ ] complex displays a fluorescence turn-on response toward the three biothiols. The UV–vis limits of detection are 0.034 μM (GSH), 0.031 μM (Cys), and 0.043 μM (Hcy), while the fluorescence limits of detection are 0.018 μM (GSH), 0.022 μM (Cys), and 0.031 μM (Hcy), respectively. More importantly, the probe J-Y has been successfully applied to test strip assays, real food sample analysis, and live-cell fluorescence imaging. Download: Download high-res image (205KB) Download: Download full-size image
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Huan Lv,Shengyan Meng,Zhaolun Cui,Shangkun Li,Dongxing Li,Xiaoxia Gao,Hongchen Guo,Annemie Bogaerts,Yanhui Yi
Efficient methane conversion to methanol remains a significant challenge in chemical industry. This study investigates the direct oxidation of methane to methanol under mild conditions, employing a synergy of non-thermal plasma and Cu-MOR (Copper-Mordenite) catalysts. Catalytic tests demonstrate that the Cu-MOR IE-3 catalyst (i.e., prepared by three cycles of ion exchange) exhibits superior catalytic performance (with 51 % methanol selectivity and 7.9 % methane conversion). Conversely, the Cu-MOR catalysts prepared via wetness impregnation tend to over-oxidize CH 4 to CO and CO 2 . Through systematic catalyst characterizations (XRD, TPR, UV–Vis, HRTEM, XPS), we elucidate that ion exchange mainly leads to the formation of zeolite-confined Cu 2+ species, while wetness impregnation predominantly results in CuO particles. Based on the catalytic performance, catalyst characterizations and in-situ FTIR spectra, we conclude that zeolite-confined Cu 2+ species serve as the active sites for plasma-catalytic direct oxidation of methane to methanol.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Xu-hong Han,Xue-wei Zhao,Kun Huang,Lei Yang,Qing Wang,Peng-fei Shi
Tracing copper ions levels in the environment and subcellular microenvironment is crucial due to the key role copper ions play in physiological and pathological processes. Herein, a novel naphthalimide-fused rhodamine probe Rh-Naph-Cu was prepared through modification with phenylhydrazine to produce a closed and non-fluorescent spirolactam. Based on the copper-induced spirolactam ring-opening and hydrolysis process, Rh-Naph-Cu can be employed as a fluorescence off–on probe for copper ions with high selectivity, high sensitivity (limit of detection: 33.0 nM), broad pH-response range (pH: 5.0–10.0), and color change visible with the naked eye. Rh-Nap-Cu could be made into test strips for the in-situ chromogenic detection of Cu 2+ . Significantly, Rh-Naph-Cu can be utilized for the detection of copper ions in living HeLa cells and zebrafish, and exhibits excellent lysosomal-targeting ability with high Pearson’s correlation coefficient (PCC) of 0.96.
研究领域:
生物化学
·
分子生物学
·
环境科学
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高影响因子
RENEWABLE ENERGY
·
2020
·
IF 6.274
Jian-Wei Liu,Qiang Li,Li-Ping Sun,Li-Hua Huo,Hui Zhao,Bassat Jean-Marc,Fourcade Sébastien,Grenier Jean-Claude
PrSrCo 1-x Cu x O 4+δ ( x = 0.0, 0.1, 0.3, 0.5) are synthesized by solid-state reaction method. The effects of Cu 2+ doping on electrical conductivity , thermal expansion, oxygen diffusion properties and oxygen reduction reaction (ORR) activity are investigated. PrSrCo 1-x Cu x O 4+δ oxides crystallize in a single-phase tetragonal structure with I4/mmm space group. Partial substitution Co 3+ by Cu 2+ results the increase of electrical conductivity and decrease of thermal expansion coefficient . Introducing Cu 2+ promotes the formation of interstitial oxygen , accelerates the kinetics of oxygen transport, and significantly improves the electrocatalytic activity of ORR. The lowest area specific resistance (ASR) value of 0.08 Ω cm 2 is obtained for the PrSrCo 0.7 Cu 0.3 O 4+δ cathode at 700 °C in air. The ORR mechanism study demonstrates that Cu-doping (30%) promotes the charge transfer process. The reaction rate control step on PrSrCo 0.7 Cu 0.3 O 4+δ cathode is the dissociation and surface diffusion process of adsorbed molecular oxygen .
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Chemical Engineering and Processing-Process Intensification
·
2019
·
IF 3.031
Yu-Jie Zhang,Juan-Qin Xue,Fei Li,JI-Zhe Dai,Xuan-Zhuo-Yi Zhang
Capacity deionization technology, an innovative desalination technology, is applied to removing heavy metal ions. As a new pseudo-capacitance electrode material, polypyrrole (PPy) is considered as a promising and perfect electrode material of CDI. In this paper, polypyrrole/chitosan/carbon nanotubes (PPy/CS/CNT) composite nano-electrode were prepared by in situ polymerization, and its surface characteristics, electrochemical properties and adsorption performances were tested. SEM and TEM images shown that CS and PPy are coated on CNT surface in order. The BET surface area and BJH pore diameter of PPy/CS/CNT composite nanoelectrode are 33.51 m 2 /g and 34.57 nm, respecitively, tested by nitrogen adsorption-desorption isotherms. Cyclic voltammetry (CV) test shown that specific capacitance value and adsorption capacity of the PPy/CS/CNT composite nanoelectrode both are increased first and then decreased with the increasing of the CS dosages, and the highest values are 103.19 F/g and 16.83 mg/g at mass ratio of CS:Py = 2:10, which are 1.6 times and 2.1 times of that of PPy/CNT composite nanoelectrode, respectively. The adsorption kinetic data followed the pseudo-second-order kinetic model, indicating a chemical adsorption process of composite electrode for heavy metal ions.
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