INTERNATIONAL IMMUNOPHARMACOLOGY
·
2023
·
IF 5.6
Wenbo Ge,Zhun Li,Yajun Yang,Xiwang Liu,Zhaohan Zhu,Lixia Bai,Zhe Qin,Xiao Xu,Jianyong Li,Shihong Li
Pathogenic Escherichia coli ( E. coli ) can cause intestinal diseases in humans and livestock, damage the intestinal barrier, increase systemic inflammation, and seriously threaten human health and the development of animal husbandry. In this study, we designed and synthesized a novel conjugate florfenicol sulfathiazole (FST) based on drug combination principles, and investigated its antibacterial activity in vitro and its protective effect on inflammatory response and intestinal barrier function in E. coli O78-infected mice in vivo . The results showed that FST had superior antibacterial properties and minimal cytotoxicity compared with its prodrugs as florfenicol and sulfathiazole. FST protected mice from lethal E. coli infection, reduced clinical signs of inflammation, reduced weight loss, alleviated intestinal structural damage. FST decreased the expression of inflammatory cytokines IL-1β, IL-6, TNF-α, and increased the expression of claudin-1, Occludin, and ZO-1 in the jejunum, improved the intestinal barrier function, and promoted the absorption of nutrients. FST also inhibited the expression of TLR4, MyD88, p-p65, and p-p38 in the jejunum. The study may lay the foundation for the development of FST as new drugs for intestinal inflammation and injury in enteric pathogen infection.
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JOURNAL OF DAIRY SCIENCE
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2022
·
IF 4.225
Bolong Fang,Juan Peng,Gan Zhang,Keyu Xing,Wenyao Chen,Daofeng Liu,Shan Shan,Yonghua Xiong,Weihua Lai
ABSTRACT Escherichia coli O157:H7 is a type of hazardous bacteria in the field of food safety. A sensitive and effective method is urgently needed to detect it, avoiding enormous harm for the human health. In this study, we synthesized stable Ag + -doped gold nanoclusters (Ag-AuNC) with a fluorescence intensity 4.8 times stronger than that of AuNC. It was further demonstrated that Ag 0 existing in the AuNC core and a fraction of Ag + anchored on the AuNC shell eliminated the surface defects and improved the luminescent properties of AuNC. A combination of I 2 and I − was used to quench fluorescence-enhanced Ag-AuNC, which was first applied in ELISA for detecting E. coli O157:H7 to improve the sensitivity. In the presence of E. coli O157:H7, the biotinylated anti- E. coli O157:H7 mAb and streptavidin-alkaline phosphatase would be immobilized and catalyze l -ascorbic acid 2-phosphate sesquimagnesium salt hydrate to produce ascorbic acid. After addition of KIO 3 , I 2 /I − were generated. The I 2 could trigger oxidative etching of Ag-AuNC and I − could combine with Ag + to decrease the Ag + concentration of Ag-AuNC, which resulted in fluorescence quenching of Ag-AuNC. Under optimal conditions, the linear range of I 2 /I − -mediated fluorescence quenching of Ag-AuNC-based immunoassay for detecting E. coli O157:H7 was 3.3 × 10 3 to 10 6 cfu/mL, with a detection limit of 9.2 × 10 2 cfu/mL, 10.7-fold lower than that of the traditional ELISA. The proposed immunoassay exhibits excellent sensitivity, specificity, recovery, and accuracy, which is useful for quantitative detection of E. coli O157:H7 in food safety.
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ACS Applied Bio Materials
·
2025
·
IF 4.7
Jinyan Du,Jinrui Hou,Shiji Liu,Xinyue Wu,Liangde Hu,Wenjiang Xu,Shujuan Zhuo
Slow or noticeably delayed wound healing is frequently intimately linked to bacterial infection and excessive reactive oxygen species (ROS), while the inappropriate usage of antibiotics fuels the rise of bacterial resistance. The innovative materials are desperately needed to eliminate bacteria and effectively accelerate wound healing. In this work, a curcumin-loaded silver-based metal–organic framework (Cur/Ag-MOF) composite nanomaterial was developed, which exhibited good antimicrobial activity, biocompatibility, and drug resistance. Meanwhile, surface-loaded curcumin can effectively eliminate excess free radicals and promote wound healing due to its antioxidative and ROS scavenging properties. Additionally, it was discovered that the application of Cur/Ag-MOF to the site of skin trauma significantly sped up the process of wound closure in mice used as subjects. These findings highlighted its great potential for treating bacterial infection-induced skin injuries and aiding the healing and reconstruction of skin tissues.
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JOURNAL OF BIOTECHNOLOGY
·
2024
·
IF 4.1
Bowen Shen,Lin Zhang,Yu Zhou,Feifei Song,Shengping You,Rongxin Su,Wei Qi
5-Hydroxytryptophan (5-HTP), a precursor of the neurotransmitter serotonin in mammals, has demonstrated efficacy in treating various diseases such as depression, fibromyalgia and obesity. However, conventional biosynthesis methods of 5-HTP are limited by low yield and high reagent and process costs. In this study, the strain C1T7-S337A/F318Y with optimized promoter distribution was obtained, and the 5-HTP yield was 60.30 % higher than that of the initial strain. An efficient fermentation process for 5-HTP synthesis was developed using strain C1T7-S337A/F318Y with whey powder as a substrate for cell growth and inducer production. Shake flask fermentation experiments yielded 1.302 g/L 5-HTP from 2.0 g/L L-tryptophan (L-Trp), surpassing the whole-cell biocatalysis by 42.86 %. Scale-up to a 5 L fermenter further increased the yield to 1.649 g/L. This fermentation strategy substantially slashed reagent cost by 95.39 %, providing a more economically viable and environmentally sustainable route for industrial biosynthesis of 5-HTP. Moreover, it contributes to the broader utilization of whey powder in various industries.
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Food and Bioprocess Technology
·
2026
·Vol. 19
·期 9
·440
·
IF 5.6
Huang Huisha,Chen Shizhuo,Xu Xinyi,Mao Xin,Shou Yiwen,Lu Qinpei,Feng Chao,Ye Yu,Guo Hongwei,Su Zhiheng,Huang Zhaoquan
Sea cucumber peptide products are valued for their high cost and potent biological activity, making it essential to monitor Escherichia coli contamination to ensure quality control. A novel sandwich assay had been developed, integrating magnetic enrichment with positive magnetic beads, magnetophoretic chromatographic separation, and nanozyme-mediated colorimetric photothermal detection to quantify Escherichia coli O157:H7 ( E. coli O157:H7). This approach used amino-functionalized magnetic nanoparticles (Fe 3 O 4 @SiO 2 -NH 2 ) for effective bacterial concentration, demonstrating substantial capture efficiency. Magnetophoretic separation further enhanced enrichment, achieving a capture efficiency exceeding 85% for high-concentration samples (10 7 CFU/mL). An aptamer specific to E. coli O157:H7, modified on V-doped manganese dioxide nanozyme encapsulated by ZIF-90 (V-MnO 2 @ZIF-90), served as the secondary binding agent, ensuring high specificity. This configuration resulted in the formation of the Fe 3 O 4 @SiO 2 -NH 2 - E. coli O157:H7-V-MnO 2 @ZIF-90/Apt sandwich complex. Upon the addition of ABTS, both colorimetric and photothermal signals were generated, yielding a detection limit of 1 × 10 CFU/mL for sea cucumber peptides samples, with the total detection time being approximately 75 min. This method presents significant promise for the rapid separation and detection of pathogenic bacteria in food products.
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Microorganisms
·
2026
·Vol. 14
·期 4
·872
·
IF 4.7
Kai Zong,Liwen Chen,Waseem Raza,Xin Wang,Lin Yang,Zhongwei Chen
Microbial fuel cells (MFCs) are a sustainable approach to wastewater treatment and energy recovery. However, their practical utility is often limited by sluggish cathode kinetics. For this technology, developing cost-effective biocatalysts that do not compromise effectiveness is a primary challenge. In this study, we utilized anthocyanin molecularly functionalizedEscherichia coli(Cya-WT) to promote the formation of electroactive biofilms and regulate the intrinsic catalytic activity of single cells, thereby enhancing extracellular electron transfer. MFCs incorporating Cya-WT-loaded carbon cloth (CC) biocathodes were configured to simultaneously evaluate power generation and glucose degradation activity. The results indicated that Cya-WT exhibited significantly improved oxygen reduction reaction (ORR) activity, achieving a reduction peak current of 3.61 mA cm−2, compared to 2.02 mA cm−2for wild-typeE.coli(WT). The assembled MFC offers a peak power density of 268 ± 13.4 μW cm−2and decomposes 17.1 ± 1.15 mM of glucose in 150 h, maintaining a consistent voltage output for 800 h. These results demonstrate that anthocyanin functionalization significantly enhances the electrocatalytic performance and metabolic capabilities ofE. coli. This novel catalyst design method offers a new strategy for low-cost, renewable MFC cathode catalysts and shows good promise in MFC biopower generation through the assembly of carbon-based biocathodes.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Xuewei Yang,Yangchun Qi,Jianxing Feng,Fei Han,Pengfei Zhang,Linpin Luo,Zeyan Zheng,Weixuan Zhang,Zhonghong Li,Wenzhi Tang
The sensitive and on-site detection of highly pathogenic Escherichia coli O157:H7 ( E. coli O157:H7) is of great importance to ensure public health. However, conventional methods for E. coli O157:H7 detection may suffer disadvantages such as high expense and time consumption. Herein, we reported a cost-effective colorimetric platform based on loop-mediated isothermal amplification (LAMP) for ultrasensitive and rapid detection of E. coli O157:H7. In this study, a novel quantitative colorimetric LAMP assay was established by in-situ measurement of hydroxy naphthol blue (HNB) color change with a designed portable device, which was applied to the amplification of the specific rfbE gene of E. coli O157:H7. As expected, this method featured a wide linear range (10 0 -10 4 copies µL −1 ) with excellent linear regression analysis (R 2 > 0.99) and possessed a record-low detection limit of 1 copy µL −1 to lower the risk of missed detection. The proposed strategy could be employed on drinking water samples with favorable recoveries and also demonstrated potential in the analysis of complex samples. This work exhibits the promising application prospect in on-site detection without the need for expensive instrumentation and seeks to provide an original paradigm for the rapid and sensitive detection of waterborne pathogens.
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TALANTA
·
2023
·
IF 6.1
Lidong Pang,Ling'e Wang,Yaqi Liang,Zhenghui Wang,Wei Zhang,Qianyu Zhao,Xinyan Yang,Yujun Jiang
Escherichia coli O157: H7 ( E. coli O157: H7) is one of the most common foodborne pathogens and is widespread in food and the environment. Thus, it is significant for rapidly detecting E. coli O157: H7. In this study, a colorimetric aptasensor based on aptamer-functionalized magnetic beads, exonuclease III (Exo III), and G-triplex/hemin was proposed for the detection of E. coli O157: H7. The functional hairpin HP was designed in the system, which includes two parts of a stem containing the G-triplex sequence and a tail complementary to cDNA. E. coli O157: H7 competed to bind the aptamer (Apt) in the Apt-cDNA complex to obtain cDNA. The cDNA then bound to the tail of HP to trigger Exo III digestion and release the single-stranded DNA containing the G-triplex sequence. G-triplex/hemin DNAzyme could catalyze TMB to produce visible color changes and detectable absorbance signals in the presence of H 2 O 2 . Based on the optimal conditions, E. coli O157: H7 could be detected down to 1.3 × 10 3 CFU/mL, with a wide linear range from 1.3 × 10 3 to 1.3 × 10 7 CFU/mL. This method had a distinguished ability to non-target bacteria, which showed good specificity. In addition, the system was successfully applied to detect E. coli O157: H7 in milk samples.
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BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2023
·Vol. 46
·期 9
·1293-1302
·
IF 3.8
Duan Zhiwen,Wang Yaowu,Ouyang Bin,Wang Pu
Ionic liquids (ILs) which synthesized from bio-renewable materials have recently attracted much attention for their applications in biocatalysis. Ethyl ( R )-3-hydroxybutyrate (( R )-EHB) as a versatile chiral intermediate is of great interest in pharmaceutical synthesis. This study focuses on evaluating the performances of choline chloride (ChCl)-based and tetramethylammonium (TMA)-based neoteric ILs in the efficient synthesis of ( R )-EHB via the bioreduction of ethyl acetoacetate (EAA) at high substrate loading by recombinant Escherichia coli cells. It was found that choline chloride/glutathione (ChCl/GSH, molar ratio 1:1) and tetramethylammonium/cysteine ([TMA][Cys], molar ratio 1:1) as eco-friendly ILs not only enhanced the solubility of water-insoluble EAA in the aqueous buffer system, but also appropriately improved the membrane permeability of recombinant E. coli cells, thus boosting catalytic reduction efficiency of EAA to ( R )-EHB. In the developed ChCl/GSH- or [TMA][Cys]-buffer systems, the space-time yields of ( R )-EHB achieved 754.9 g/L/d and 726.3 g/L/d, respectively, which are much higher than neat aqueous buffer system (537.2 g/L/d space-time yield). Meanwhile, positive results have also been demonstrated in the bioreduction of other prochiral ketones in the established IL-buffer systems. This work exhibits an efficient bioprocess for ( R )-EHB synthesis under 325 g/L (2.5 M) substrate loading, and provides promising ChCl/GSH- and [TMA][Cys]-buffer systems employed in the biocatalysis for hydrophobic substrate.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2016
·Vol. 23
·期 13
·13458-13466
·
IF 2.76
Hong Xiaoting,Li Min,Shan Shengdao,Hui K. S.,Mo Mingyue,Yuan Xiaoli
Visible light-driven photocatalytic inactivation of Escherichia coli was performed using hydroxyapatite-supported Ag 3 PO 4 nanocomposites (Ag 3 PO 4 /HA). The antibacterial performance was evaluated by the methods of zone of inhibition plates and minimum inhibitory concentration test. X-ray diffraction (XRD) and transmission electron microscopy (TEM) were employed to investigate the instability and transformation of the nanocomposite by comparing the crystalline, phase, and the morphology before and after exposure to Luria-Bertani culture medium under visible light irradiation. Ag 3 PO 4 nanoparticles on the support were found to be shortly transformed into AgCl due to high chloride concentration of Luria-Bertani culture medium. The AgCl/HA nanocomposite showed both excellent intrinsic antibacterial performance contributed by the released silver ions and visible light-induced photocatalytic disinfection toward E. coli cells. This dual antibacterial function mechanism was validated by trapping the hydroxyl free radical and detecting the silver ions during the photocatalytic antibacterial process. The morphological change of E. coli cells in different reaction intervals was obtained by scanning electron microscopy (SEM) to complementally verify photocatalytic inactivation of E. coli . This work suggests that an essential comparison study is required for the antibacterial materials before and after the photocatalytic inactivation of bacterial cells using Ag 3 PO 4 nanoparticles or Ag 3 PO 4 -related nanocomposites in mediums containing high-concentration chloride ions.
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CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2013
·
IF 0.769
Xi CUI,Qi-Rong XIONG,Yong-Hua XIONG,Shan SHAN,Wei-Hua LAI
In this study, immunomagnetic beads were prepared by coupling anti- Escherichia. coli O157:H7 monoclonal antibodies with magnetic beads. A colloidal gold test strip was composited with anti- E. coli O157:H7 monoclonal antibody marked by colloidal gold as the detection antibody, anti- E. coli O157:H7 polyclonal antibody as the test line, and donkey anti-mouse IgG secondary antibody as the control line. Immunomagnetic separation was coupled with colloidal gold lateral flow assay to detect E. coli O157:H7 rapidly. Results showed that 1 mL 7.6 × 10 3 colony-forming unit (CFU) mL 1 E. coli O157:H7 exhibited a positive outcome when colloidal gold lateral flow assay was performed after immunomagnetic separation. A food sample inoculated with 10 CFU/g E. coli O157:H7 after 6 h of enrichment could be detected within 72 min of immunomagnetic separation and colloidal gold lateral flow assay. The method proposed in this study could be used to on-site detect harmful substances in food significantly and rapidly.
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FOOD CONTROL
·
2024
·
IF 5.6
Lidong Pang,Qunchao Su,Jingqi Cheng,Biqi Liu,Chaoxin Man,Ling Guo,Xinyan Yang,Qianyu Zhao,Yujun Jiang
The development of a simple, rapid, sensitive, and accurate method for detecting Escherichia coli O157:H7 in complex matrices is critical for ensuring food safety. In this study, a fluorescent biosensor employing aptamer (Apt) -functionalized magnetic beads and cascade strand displacement amplification (SDA) was devised for the detection of E. coli O157:H7 in milk samples. E. coli O157:H7 is captured by the aptamer, causing the complementary DNA (cDNA) to not bind to the aptamer and to be released into the supernatant. Following magnetic separation, the cDNA present in the supernatant initiates the opening of the hairpin (HP) structures. This enables primers to attach to the opened HP, triggering the initial SDA process, which generates a substantial quantity of double-stranded DNA (dsDNA) and recycles cDNA to open more HP structures. The dsDNA activates a second SDA, producing single-stranded DNA (ssDNA) for template binding and instigating a third SDA process. Triple SDA leads to the production of numerous G-quadruplex sequences and the formation of a G-quadruplex structure in the presence of K + . The inclusion of ThT into the G-quadruplex significantly enhances fluorescence. Under optimal conditions, this approach can detect E. coli O157:H7 at levels as low as 10 1 CFU/mL in pure cultures within 2.5 h, with a broad linear detection range of 10 1 –10 6 CFU/mL. This method exhibits remarkable specificity towards the target bacteria over non-target bacteria. Furthermore, the technique has been effectively utilized for the detection of E. coli O157:H7 in milk samples. Notably, the method involves only two main steps, identification and amplification, significantly reducing contamination risks and minimizing operational steps.
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