Communications Biology
·
2024
·Vol. 7
·期 1
·1-17
·
IF 5.2
Wang Yi,Fu Xiaolong,Zeng Li,Hu Yan,Gao Rongyang,Xian Siting,Liao Songjie,Huang Jianxiang,Yang Yonggang,Liu Jilong,Jin Hai,Klaunig James,Lu Yuanfu,Zhou Shaoyu
Nuclear factor erythroid 2-related factor-2 (Nrf2) antioxidant signaling is involved in liver protection, but this generalization overlooks conflicting studies indicating that Nrf2 effects are not necessarily hepatoprotective. The role of Nrf2/heme oxygenase-1 (HO-1) in cholestatic liver injury (CLI) remains poorly defined. Here, we report that Nrf2/HO-1 activation exacerbates liver injury rather than exerting a protective effect in CLI. Inhibiting HO-1 or ameliorating bilirubin transport alleviates liver injury in CLI models. Nrf2 knockout confers hepatoprotection in CLI mice, whereas in non-CLI mice, Nrf2 knockout aggravates liver damage. In the CLI setting, oxidative stress activates Nrf2/HO-1, leads to bilirubin accumulation, and impairs mitochondrial function. High levels of bilirubin reciprocally upregulate the activation of Nrf2 and HO-1, while antioxidant and mitochondrial-targeted SOD2 overexpression attenuate bilirubin toxicity. The expression of Nrf2 and HO-1 is elevated in serum of patients with CLI. These results reveal an unrecognized function of Nrf2 signaling in exacerbating liver injury in cholestatic disease.
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RESEARCH ON CHEMICAL INTERMEDIATES
·
2024
·
IF 2.8
Xiong Neng,Jiang Lin-Li,Chen Jia-Yu,Lin Lei,Huang Jin-Rong,Shen Qi,Xue Ya-Ping,Zheng Yu-Guo
Hydrogenation plays an important role in the production of bulk and fine chemicals. In the present work, the hydrogenation process of 1-cyanocyclohexaneacetic acid (1-CA), a key intermediate in the chemo-enzymatic synthesis of the antiepileptic drug gabapentin, was investigated. The catalysts were screened, the operating parameters for the 1-CA hydrogenation were determined by response surface methodology, and the effects of biological impurities on the reaction and catalysts were investigated. The reaction temperature, reaction solution pH and reaction time were identified as the main factors for hydrogenation by single-factor experiments. Under the optimal reaction conditions: reaction temperature 110 °C, solution pH 12.33, reaction time 3.74 h, hydrogen pressure 2 MPa and stirring speed 500 rpm, the substrate conversion reached 100%, and the total product yield reached 96.40%, which was very close to the prediction (96.88%). It was demonstrated that biological impurities greatly affect the reaction rate of hydrogenation, as well as the activity and reusability of the catalyst, in which protein impurities lead to catalyst deactivation through cumulative deposition on the catalyst surface and within the pores.
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INORGANIC CHEMISTRY
·
2024
·
IF 4.3
Jing-ran Yu,Pan-pan Zhang,Jia-yu Ding,Yue-bin Liu,Wei-hai Lin,Wei Shao,Jia-jia He,Qihan Gong,Ming Xue
The separation of high-octane dibranched alkanes from naphtha is critical in the refining of gasoline. To date, research on the membrane-based separation of alkane isomers has been limited, with a particular paucity of investigations into mixed-matrix membranes. Herein, the continuous and dense UiO-66/PIM-1 mixed-matrix membrane, which was prepared through precise control of the interfacial structure, was first applied to the differentiation of C6 alkane isomers. Due to the synergistic combination of UiO-66 with differential adsorption capabilities for alkanes and PIM-1 that possesses a cross-linkable structure, the resulting UiO-66/PIM-1-(20) membrane demonstrated remarkable separation performance and high stability. Pervaporation measurements showed that the mass fraction of 2,2-dimethylbutane in the feed side was increased from 50.0 to 75.8 wt % while an excellent flux of 1700 g m–2 h–1 was maintained over a continuous 40 h period. The UiO-66/PIM-1-(20) membrane, characterized by its facile replication and processing, shows potential for large-scale fabrication. This study offers a new approach to the membrane separation of alkane isomers.
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SusMat
·
2025
·
IF 21.3
Xuechun Hu,Yuchen Pang,Chaoqun Li,Xiaoyue Zhang,Xuebin Yu,Fang Fang,Guanglin Xia
Magnesium hydride (MgH 2 ) has been regarded as an attractive candidate for solid-state hydrogen storage, yet its practical applications are limited by the requirement of elevated temperatures and sluggish hydrogen uptake and release kinetics. Herein, TiO 2 polyhedral frameworks with uniformly distributed V 2 O 5 (denoted as V 2 O 5 /TiO 2 ) are constructed to improve hydrogen storage performance of Mg/MgH 2 . During the reversible hydrogenation and dehydrogenation process, metallic V and Ti along with low-valent Ti- and V-based oxides are in situ formed. Among them, metallic V supported on TiO 2 exhibits the lowest hydrogen adsorption energy, enabling superior catalytic performance over TiO 2 and V 2 O 5 . As a result, the peak dehydrogenation temperature of MgH 2 decreases to 215°C, 105°C lower than that of pristine MgH 2 , with a decrease of the apparent activation energy from 139.50 kJ·mol −1 to 68.99 kJ·mol −1 . Moreover, electron migration from V toward TiO 2 leads to charge accumulation around Ti and O atoms, shifting the V 3 d -band center toward the Fermi level and thereby improving the catalytic function of V's d-electrons, facilitating hydrogen dissociation without energy barriers. Therefore, the V 2 O 5 /TiO 2 -catalyzed Mg absorbs 4.12 wt% H 2 under an ultralow pressure of 1 bar at 25°C. This provides a new strategy for developing advanced Ti and V-based catalysts for mild-condition hydrogen storage of MgH 2 .
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Jingyi Chen,Xuan Wang,Xuemei Yuan,Haiting Zou,Jiaye Li,Guopu Chen,Qian Tan
Fibroblast dysfunction is a key factor in the prolonged healing of diabetic wounds. Netrin-1 was found to be able to ameliorate high-glucose-induced fibroblast dysfunction. In this study, we developed a biomimetic dual-network hydrogel loaded with Netrin-1 to promote diabetic wound healing. The hydrogel was composed of natural ingredients, sodium alginate (SA) and glycyrrhizic acid (GA). In addition, with the octopus’s suction cup structure on the surface, this hydrogel exhibited strong adhesion properties and provided protective and sustained release of Netrin-1. The released could promote autophagy by activating the AMPK-mTOR pathway under sustained hyperglycemic conditions, thereby reversing fibroblast dysfunction and restoring cell migration and collagen synthesis. The resultant SA/GA@Netrin-1 hydrogel not only possessed potent antioxidant and antimicrobial effects but also enhanced collagen deposition in diabetic rat wounds, attenuated the inflammatory response, promoted autophagy, and significantly accelerated wound closure. These findings suggest that the SA/GA@Netrin-1 hydrogel provided an innovative solution for chronic diabetic wound management and has the potential to be applied to other refractory wound therapies.
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VETERINARY MICROBIOLOGY
·
2025
·
IF 2.7
Qing Yang,Shengda Xie,Yundi Zhao,Xinxin Lin,Ning Wei,Miaolei Shi,Ruibing Cao
Japanese encephalitis virus (JEV) is a zoonotic pathogen that can be transmitted to humans and animals via arthropods, causing viral encephalitis. In this study, we investigated the role of transferrin receptor 1 (TfR1) in JEV infection. We found that knocking down TfR1 expression in A549 and HeLa cells significantly inhibited JEV entry, while overexpression of TfR1 in 293 T cells enhanced viral entry. The JEV E protein interacts with TfR1, specifically via the protease-like domain (residues 384–606). Additionally, the hTfR1 soluble ectodomain protein significantly blocked JEV infection in HeLa cells, and ferric ammonium citrate (FAC), an iron supplement, downregulated TfR1 expression and effectively inhibited JEV entry and infection. These findings identify TfR1 as an entry factor for JEV and a potential antiviral target, suggesting that small molecule inhibitors like FAC could offer a promising antiviral strategy.
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高影响因子
AMERICAN JOURNAL OF THE MEDICAL SCIENCES
·
2022
·
IF 3.462
Qi Wang,Yangfan Li,Chuanqiang Wu,Tong Wang,Ming Wu
Background Ischemia-reperfusion injury (IRI), which involves severe inflammation and edema, is an inevitable feature of the lung transplantation process and leads to primary graft dysfunction (PGD). The activation of aquaporin 1 (AQP1) modulates fluid transport in the alveolar space. The current study investigated the role of AQP1 in ischemia-reperfusion (IR)-induced lung injury. Methods A mouse model of lung IR was established by clamping the left lung hilar for 1 h and released for reperfusion for 24 h. The AQP1 inhibitor acetazolamide (AZA) was administered 3 days before lung ischemia with a dose of 100 mg/kg per day via gavage. Lung injury was evaluated using the ratio of wet-to-dry weight, peripheral bronchial epithelial thickness, degree of angioedema , acute lung injury score, neutrophil infiltration , and cytokine concentrations in bronchoalveolar lavage fluid . Results Compared with sham treatment , ischemia with no reperfusion (IR 0h) and ischemia with reperfusion for 24 h (IR 24 h) significantly upregulated AQP1 expression, increased the wet/dry weight ratio, angioedema, neutrophil infiltration and cytokine production (interleukin -6 and tumor necrosis factor -α) and thickened the peripheral bronchial epithelium . AZA exacerbated inflammation and pulmonary edema. Conclusion AQP1 may exert a protective effect against IR-induced lung injury, which could be attributed to alleviating pulmonary edema and inflammation. AQP1 upregulation might be a potential application to alleviate lung IRI and decrease the incidence of PGD.
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Microbial Cell Factories
·
2021
·Vol. 20
·期 1
·1-11
·
IF 5.328
Wan Yupeng,Liu Hongchen,Xian Mo,Huang Wei
Background 1-Hydroxyphenazine (1-OH-PHZ) is a phenazine microbial metabolite with broad-spectrum antibacterial activities against a lot of plant pathogens. However, its use is hampered by the low yield all along. Metabolic engineering of microorganisms is an increasingly powerful method for the production of valuable organisms at high levels. Pseudomonas chlororaphis is recognized as a safe and effective plant rhizosphere growth-promoting bacterium, and faster growth rate using glycerol or glucose as a renewable carbon source. Therefore, Pseudomonas chlororaphis is particularly suitable as the chassis cell for the modification and engineering of phenazines. Results In this study, enzyme PhzS (monooxygenase) was heterologously expressed in a phenazine-1-carboxylic acid (PCA) generating strain Pseudomonas chlororaphis H18, and 1-hydroxyphenazine was isolated, characterized in the genetically modified strain. Next, the yield of 1-hydroxyphenazine was systematically engineered by the strategies including (1) semi-rational design remodeling of crucial protein PhzS, (2) blocking intermediate PCA consumption branch pathway, (3) enhancing the precursor pool, (4) engineering regulatory genes, etc. Finally, the titer of 1-hydroxyphenazine reached 3.6 g/L in 5 L fermenter in 54 h. Conclusions The 1-OH-PHZ production of Pseudomonas chlororaphis H18 was greatly improved through systematically engineering strategies, which is the highest, reported to date. This work provides a promising platform for 1-hydroxyphenazine engineering and production. Graphical Abstract
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Future Medicinal Chemistry
·
2022
·Vol. 14
·期 20
·
IF 4.767
Haijun Gu,Ting Zhang,Yunqi Li,Yuan He,Tian Guan,Weiqiong Kan,Peng He,Zhengfang Yi,Yihua Chen
Aim: The first inhibitor targeting myoferlin (MYOF), WJ460, bears poor metabolic stability and water solubility. Therefore, this study aimed to improve the drug-like properties of WJ460. Materials & methods: The authors synthesized an array of 1,5-diaryl-1,2,4-triazole analogs and appraised the binding activities with MYOF and their antiproliferative and antimigratory activities against pancreatic cancer cells. Results: Molecular docking and surface plasmon resonance results showed that E4 was directly bound to the MYOF-C2D domain. E4 effectively inhibited the proliferation and migration of pancreatic cancer cells in vitro. An in silico study suggested that the water solubility of E4 was improved by about 22-times than that of WJ460. Conclusion: The findings suggested that the druglike ability of E4 was significantly improved.Graphical abstract
研究领域:
药理学
·
肿瘤学
·
药物化学
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FirePhysChem
·
2022
·
IF 0
Kunkai Wang,Xiangzhi Li,Kaidi Yang,Huan Huo,Qi Xue,Bozhou Wang,Fuqiang Bi
1,1,4,4-Tetramethyl-2-tetrazene (TMTZ), a potential candidate for replacing 1,1-dimethylhydrazine (UDMH), has excellent properties including a high specific impulse, non-toxicity and non-volatility. However, most synthetic methods depicted in the literature use stoichiometric oxidative coupling, and the oxidizing agents are usually heavy metals or halogens, leading to low synthetic efficiency and high environmental pollution. In order to overcome these problems, it is necessary to explore more environmentally friendly and catalytic synthetic methods. In this paper, a method of photocatalytic oxidative coupling for the preparation of TMTZ was reported for the first time. By using an organic photocatalyst Eosin Y, TMTZ was synthesized rapidly and efficiently. The thermal decomposition properties of TMTZ were studied, and different performance characteristics between TMTZ and UDMH were determined and analyzed, which provided a basis for the application of TMTZ based new liquid propellant.
研究领域:
有机化学
·
催化化学
·
能源材料
·
推进剂化学
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ZEITSCHRIFT FUR KRISTALLOGRAPHIE-NEW CRYSTAL STRUCTURES
·
2023
·Vol. 238
·期 3
·471-473
·
IF 0.3
Li Liu,Huan-Mei Guo,Li-Li Liu,Xin-Ru Qian
C 27 H 26 N 9 O 10 Pr, monoclinic, P 2 1 / c , a = 13.2280(12) Å, b = 5.5628(5) Å, c = 41.140(3) Å, β = 105.109(2)°, V = 2922.6(4) Å 3 , Z = 4, R gt
( F ) = 0.0426, w R ref
( F
2 ) = 0.0803, T = 298.15 K.
研究领域:
化学
·
材料科学
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Renjie Zhou,Jiahao Yu,Shuo Li,Jian Zhang,Chenqiang Wang,Lianfu Zhang
Amadori compounds (ACs; N-(1-deoxy-d-fructos-1-yl)-amino acid) are superior flavor precursors and potential functional ingredients in food processing. In this study, vacuum dehydration as an excellent and universal method for the formation of ACs in both simulation systems and food processing was revealed. In total, 12 amino acids referring to all six categories were selected to conduct simulated reactions with glucose in aqueous models. At 90 °C, yields of 11 ACs were significantly increased by vacuum dehydration, reaching 4–198 times compared to a heat sealing reaction in aqueous systems, and formation of 5-hydroxymethyl-2-furaldehyde (5-HMF) and browning were slower than that by a dry powder reaction. In particular, the yields of Fru-Arg, Fru-His, and Fru-Glu reached 87.03, 90.73, and 89.88 mol %, respectively. The order of promotion effect was acid ACs > basic ACs > unique ACs > polar neutral ACs > aliphatic ACs > aromatic ACs. The excellent effect was mainly attributed to the control of water activity (Aw) and pH, which enabled the models to reach the optimal reaction state quickly by adjusting the vacuum degree at mild temperatures. The method was also applied to AC enrichment in tomato sauce processing; the AC content could rise to 30.72 mg/g, which was more than 17 times than those in samples without vacuum dehydration and two commercial tomato sauces.
研究领域:
食品科学
·
化学
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危险品化学品经营许可证（带存储）