SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Daqi Chen,Huiyan Li,Xuefei Su,Nan Li,You Wang,Adrian Carl Stevenson,Ruifen Hu,Guang Li
Continuous glucose monitoring (CGM) in vivo is required for tight glycaemic control. Enzyme-free glucose sensors were proposed to solve the problems of daily invasive calibration and short lifespan of the current enzyme based continuous glucose monitors. In this report, a wireless-electrodeless quartz crystal microbalance method that tracks film dissipation was developed and proposed as a basis for a miniaturized implantable glucose monitor. An AT-cut blank quartz disc (14 mm in diameter) was stimulated wirelessly by a planar copper coil and working in the ring-down mode at a fixed frequency point (˜6 MHz). A 600–800 nm (3-acrylamidopropyl) tri-methylammonium chloride modified poly(acrylamide- co -3-acrylamideophenylboronic acid) hydrogel film was coated on the quartz disc for glucose sensing. The viscosity variations of the polymer film induced by the glucose binding were observed by measuring the dissipation change of the sensor. The linear relationship between the dissipation response and the glucose concentration was achieved in the range of ˜0 to 10 mM, with a sensitivity of 2 × 10 −5 mM −1 and a response time of around 5 min at 37 °C. Furthermore, the response of the modified film to other interferences including the fructose, galactose, mannose, uric acid was dramatically reduced. The results suggest the wireless-electrodeless quartz crystal microbalance with dissipation system has the potential to be used as a subcutaneously implanted real-time glucose monitor.
✓ 已验证
产品关联
DOI
高影响因子
Biomed Research International
·
2019
·
IF 2.197
Hao Lu,Hui Yao,Rong Zou,Xiwen Chen,Hanlin Xu
Renal inflammation can result in renal injury. Uric acid (UA) is the final product of purine metabolism in humans and because of the lack of urate oxidase, UA may accumulate in tissues, including kidney, causing inflammation. Galangin was isolated from a traditional Chinese medicine plant and possesses several beneficial effects, working as an anti-oxidant, anti-mutagenic, anti-tumor, anti-inflammatory, anti-microbial, and anti-viral agent. Therefore, this study aimed at investigating the molecular mechanism of galangin in the attenuation of UA induced renal inflammation in normal rat kidney epithelial cells NRK-52E. Our findings suggested that galangin treatment efficiently protected NRK-52E cells against UA induced renal inflammation by decreasing tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-18, prostaglandin E2 (PGE2), and nitric oxide (NO) release, and it inhibited nitric oxide synthase (iNOS), prostaglandin endoperoxide synthase 2 (PTGS2), TNF-α, IL-1β, and IL-18 mRNA expression. In addition, galangin was not exerting any cytotoxicity at the concentrations that were effective against inflammation as assessed by CCK8 assay. Moreover, western blotting showed that galangin treatment effectively inhibited nuclear factor-kappa B (NF-κB), phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) and nucleotide-binding domain- (NOD-) like receptor protein 3 (NLRP3) signaling pathway activation. Taken together, these findings suggested that galangin plays a pivotal role in renal inflammation by suppressing inflammatory responses, which might be closely associated with the inhibition of NLRP3 inflammasome, NF-κB and PI3K/AKT signaling pathway activation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-8
·
IF 5.479
Li Yuanyuan,Du Qianqian,Zhang Xiaodan,Cao Haiyan,Huang Yuming
The authors describe the preparation of gold nanoclusters (AuNCs) capped with kojic acid. The capped AuNCs exhibit bright green fluorescence (peaking at 500 nm upon excitation at 375 nm), a nanosecond lifetime (0.37 ns), and a quantum yield (QY) of 22% in aqueous solution. This is higher than most of the previously reported AuNCs. The QY increases to 58% due to aggregation-induced emission in ethanol solution, and the lifetime is prolonged to 1.3 ns. The fluorescence of the KA-AuNCs is quenched by Eu(III) ion but is recovered by addition of phosphate due to its stronger affinity for Eu(III). Under the catalytic action of alkaline phosphatase (ALP), ascorbic acid phosphate (AAP) is transformed to free phosphate. On this basis, a fluorogenic assay for ALP was established. Response is linear in the 0.2 to 20 U·L −1 activity range, and the detection limit is 0.04 U·L −1 (at S/ N = 3). The assay was successfully applied to the determination of the activity of ALP in spiked human serum and also to screen for its inhibitors.
Highly luminescent and stable gold nanoclusters (AuNCs) with aggregation-induced emission property were synthesized through non-thiolate ligand kojic acid (KA) and demonstrated as an efficient probe for screening for alkaline phosphatase (ALP) activity and its inhibitors.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-10
·
IF 5.479
Zhu Yanyan,Zhang Xuan,Sun Jiameng,Li Meng,Lin Yulong,Kang Kai,Meng Yang,Feng Zhongliang,Wang Jing
Ultrafine platinum nanoparticles are grown on a 3D graphene framework (GF–Pt) via a hydrothermal method. The material, when placed on a glassy carbon electrode (GCE), displays enhanced electrocatalytic activity towards glucose oxidation. This is assumed to be the result of the numerous easily accessible active sites, an enlarged electrochemically active area, and the presence of multiple electron/ion transport channels. The modified GCE can be operated at a low potential (− 0.15 V vs. Ag/AgCl) has linear responses in the 0.1 μM − 0.01 mM and 0.01 mM – 20 mM glucose concentration range, and a 30 nM detection limit. It was applied to the rapid determination of glucose in human serum samples.
Schematic presentation of a glassy carbon electrode modified with ultrafine Pt nanoparticles grown on a graphene framework (GFs–Pt). GFs–Pt presents enhanced electrocatalytic activity towards glucose oxidation. GFs–Pt is used in a sensitive non-enzymatic amperometric glucose sensor.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2019
·
IF 5.108
Min Liu,Jiayue Dong,Yawen Shen,Chunyong Zhang,Degang Fu
In the present study, the mineralization performance and pathway of uric acid (UA) on boron-doped diamond (BDD) anodes were investigated. The oxidation behavior of UA on BDD surface was firstly tested through cyclic voltammetry measurements. The individual and joint effects of four quantitative parameters (applied current density, NaHCO 3 concentration, NaCl concentration and flow rate) on UA mineralization were then examined with Doehlert experimental design. The results acquired by statistical analysis revealed that NaCl concentration and applied current density displayed the most dominant roles on UA degradation, while the influences of NaHCO 3 concentration and flow rate were statistically insignificant. As a result, the following optimal conditions were reached: applied current density of 7.80 mA cm −2 , NaHCO 3 concentration of 6.0 mM, NaCl concentration of 9.0 mM and flow rate of 600 mL min −1 , which gave a TOC decay of 89.4%, a specific energy consumption of 125.36 KWh kg −1 TOC, a combustion current efficiency of 15.0% and an electrical energy per order of 35.79 KWh m −3 order −1 within 30 min of electrolysis. Further results from LC/MS analysis confirmed the ring rupture of UA during the electrolysis, due to the attack of hydroxyl radicals and active chlorine species. Accordingly, two plausible degradation pathways of UA in bicarbonate and chloride media on BDD anode were proposed respectively.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2019
·
IF 2.584
Shan Huang,Erli Yang,Jiandong Yao,Xu Chu,Yi Liu,Yue Zhang,Qi Xiao
Detection of cholesterol and uric acid biomarkers is of great importance for clinical diagnosis of several serious diseases correlated with their variations in human blood serum. In this study, a new kind of well selective and highly sensitive ratiometric fluorescent probe for cholesterol and uric acid determination in human blood serum was innovatively developed on the basis of the inner filter effect (IFE) process of nitrogen, cobalt co-doped carbon dots (N,Co-CDs) with 2,3-diaminophenazine (DAP). DAP was the oxidative product during the oxidation reaction between o-phenylenediamine and H2O2. Fluorescent magnetic N,Co-CDs possessing blue emission and magnetic property were prepared through a facile one-pot hydrothermal strategy by using citric acid, diethylenetriamine, and cobalt(II) chloride hexahydrate as precursors. N,Co-CDs exhibited good ferromagnetic property and excellent optical properties even in extremely harsh environmental conditions, implying the huge potential applications of such N,Co-CDs in biological areas. On the basis of the IFE process between N,Co-CDs and DAP, N,Co-CDs were applied to establish ratiometric fluorescent probes for the indirect detection of cholesterol and uric acid that participated in enzyme-catalyzed H2O2-generation reactions. The established IFE-based fluorescent probes exhibited relatively low detection limits of 3.6 nM for cholesterol and 3.4 nM for uric acid, respectively. The fluorescent probe was successfully utilized for the determination of cholesterol and uric acid in human blood serum with satisfying results, which provided an informed perspective on the applications of such doped CDs to explore the specific and sensitive nanoprobe in disease diagnoses and clinical therapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2019
·Vol. 144
·期 18
·5624-5636
·
IF 4.019
Long Zhao,Zhanlin Zhang,Maohua Chen,Yuan Liu,Tao Wang,Xiaohong Li
Challenges remain in the facile, rapid and sensitive detection of substances at ultralow levels. In the current study, visual sensors of hydrogen peroxide (H2O2) and choline are developed via the integration of an ultrafine fibrous substrate and self-propagating and aggregation-induced emission (AIE) probes. Self-immolative probes (SIPs) composed of phenylboronic acid triggers and choline units are grafted on electrospun polyethylene terephthalate (PET) fibers, followed by electrostatic adsorption of tetraphenylethene derivatives (TPE-SO3) to obtain fluorescent PET-Ch/TPE fibers. Choline oxidase (ChOX) is immobilized on polystyrene-co-maleic anhydride (PSMA) fibers to obtain PSMA-ChOX, followed by assembly into PET-Ch/TPE@PSMA-ChOX composite mats. The presence of H2O2 initiates the cleavage of phenylboronic acid triggers in SIPs to release choline and choline/TPE complexes from PET-Ch/TPE fibers. The released choline is oxidized by PSMA-ChOX fibers to generate H2O2 that then activates a cascade of self-propagating reactions until the release of all choline/TPE complexes, leading to the alleviation of AIE effect and gradual fluorescence fading of fibrous mats. Thus, the hydrogen peroxide and choline concentrations can be read out from the fluorescence fading time of fibrous mats with a detection limit of 0.5 μM H2O2 within 30 min, providing potential self-test devices for a real-time, naked-eye and sensitive detection of bioactive substances.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Catalysts
·
2019
·Vol. 9
·期 8
·653
·
IF 3.444
Huaiyin Chen,Huaijin Chen,Ruoyu Hong
Herein, MoS2nanoslices were simply prepared by using ultrasonic treatment, and were further decorated with Au nanoparticles (AuNPs) through an electrodeposition process to obtain the MoS2/Au nanocomposites. The obtained nanocomposites display synergetic electrocatalytic effect for the oxidation of dopamine due to the large surface area and two-dimensional structure of the MoS2nanoslices, combining with the high catalytic activity and good conductivity of AuNPs. An electrochemical sensor was constructed based on MoS2/Au-modified carbon paste electrode, for sensitive and quantitative determination of dopamine. The prepared electrochemical sensor proves excellent analytical performances: very high sensitivity, wide linear ranges (0.5–300 μM), and low detection limit (76 nM). Moreover, the dopamine sensor also displays high selectivity, good reproducibility and stability, and can be used in real sample analysis. The method of fabricating high-efficiency electrocatalysts and electrochemical sensors proposed in this study provides a good reference for developing more functionalized nanocomposites and for extending practical applications.Keywords:dopamine;MoS2;AuNPs;electrocatalyst;sensor
✓ 已验证
产品关联
DOI
高影响因子
MATERIALS LETTERS
·
2019
·
IF 3.019
Taotao Liang,Xiaogang Guo,Jing Wang,Yan Wei,Daixiong Zhang,Shuying Kong
Herein, the novel ficus-iacertiolia fruit-derived porous graphene-like nanosheets (PGNSs) with rich active functional groups were synthesized via a high-efficient and low-cost one-step carbonization technique for H 2 O 2 biosensor. Target electrode materials have promising porous structures with a large specific surface area (S a ) of 480.67 m 2 g −1 . The modified glassy carbon electrode (PGNSs/GCE) was constructed and exhibited excellent electrocatalytic activity, showing a high-efficiency detection of H 2 O 2 , high sensitivity of 6.17 μA mM −1 cm −2 , a low detection limit of as low as 2.75 μM and a wide linear range of 0.2–19 mM. Thus, this work demonstrates fundamental insights for the porous and active functional groups on electrodes, and provides a facile route to fabricate a highly sensitive H 2 O 2 sensor for practical aspergillus flavus diagnosis.
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2019
·Vol. 43
·期 26
·10517-10522
·
IF 3.069
Shasha Pang,Xianwen Kan
Developing an accurate and sensitive method for the detection of environmental pollutants is of great significance. However, traditional single signal based electrochemical sensors are usually susceptible to external and internal effects. To overcome these influences, in the present work, ferrocene (Fc) was covalently functionalized on multi-walled carbon nanotubes (MWNTs) to fabricate a facile electrochemical sensor for the detection of o-nitrophenol (o-NP) and p-nitrophenol (p-NP). With an increase in the concentration of both the nitrophenols, the peak currents of nitrophenols increased while the peak current of Fc remained constant. Thus, Fc can be used as an inner reference element to obtain a ratiometric signal by dividing nitrophenol current by Fc current, which endowed the sensor with a built-in correction of environmental effects. Moreover, Fc can effectively separate the reduction peaks of o-NP and p-NP, which made it possible to detect o-NP and p-NP individually and simultaneously. Due to the self-calibration based on the ratiometric strategy and the good conductivity of MWNTs, the prepared sensor showed high accuracy, reliability, and sensitivity for o-NP and p-NP detection with the linear range of 1.0 × 10−7 to 1.0 × 10−4 M. The facile, low cost, and sensitive sensor reported here has promising potential for accurate and reliable detection of other compounds.
✓ 已验证
产品关联
DOI
高影响因子
Nanomaterials
·
2019
·Vol. 9
·期 6
·847
·
IF 4.034
Xuan Wan,Shihui Yang,Zhaotian Cai,Quanguo He,Yabing Ye,Yonghui Xia,Guangli Li,Jun Liu
This study reports facile synthesis of MnO2nanoflowers/N-doped reduced graphene oxide (MnO2NFs/NrGO) composite and its application on the simultaneous determination of dopamine (DA) and uric acid (UA). The microstructures, morphologies, and electrochemical performances of MnO2NFs/NrGO were studied using X-ray diffraction (XRD), scanning electron microscopy (SEM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS), respectively. The electrochemical experiments showed that the MnO2NFs/NrGO composites have the largest effective electroactive area and lowest charge transfer resistance. MnO2NFs/NrGO nanocomposites displayed superior catalytic capacity toward the electro-oxidation of DA and UA due to the synergistic effect from MnO2NFs and NrGO. The anodic peak currents of DA and UA increase linearly with their concentrations varying from 0.2 μM to 6.0 μM. However, the anodic peak currents of DA and UA are highly correlated to the Napierian logarithm of their concentrations ranging from 6.0 μM to 100 μM. The detection limits are 0.036 μM and 0.029 μM for DA and UA, respectively. Furthermore, the DA and UA levels of human serum samples were accurately detected by the proposed sensor. Combining with prominent advantages such as facile preparation, good sensitivity, and high selectivity, the proposed MnO2NFs/NrGO nanocomposites have become the most promising candidates for the simultaneous determination of DA and UA from various actual samples.Keywords:dopamine;uric acid;MnO2nanoflowers;N-doped reduced graphene oxide;voltammetric sensor
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2019
·
IF 5.383
Fen Liu,Xianwen Kan
In this work, a novel dual-analyte electrochemical sensor was developed based on the combination of boronate affinity and indicator displacement assay (IDA) strategy. Reduced graphene oxidation (RGO) and poly(3-aminophenylboronic acid) (pAPBA) were modified on glassy carbon electrode (GCE), which further bound dopamine (DA) via a reversible covalent interaction between the APBA and DA. Due to the higher affinity of pAPBA to the analytes, the bound DA can be displaced by fructose (Fru) or Alizarin Red S (ARS). As an indicator, the decrease of DA current caused by the displacement was used to monitor the concentration of the analyte. For non electro-active molecule, the decrease of DA current was directly proportional to the logarithm of Fru concentration in a range from 7.0 × 10 −8 to 1.0 × 10 −4 mol/L. For electro-active molecule, the current of DA decreased along with the increase of ARS current owing to the displacement, which were combined as a dual-signl for ARS sensitive detection with a linear range of 9.0 × 10 −8 - 1.0 × 10 −5 mol/L. The results of determining analytes with their interferences demonstrated the good specific affinity of the sensor toward the analytes. The dual-analyte electrochemical sensor based on IDA strategy probably provides a potential guidance for multi-analyte facile, sensitive, and specific detection.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）