ACTA BIOCHIMICA ET BIOPHYSICA SINICA
·
2020
·Vol. 52
·期 5
·563-569
·
IF 2.836
Wang Lianqing,Lan Yongting,Du Yanqin,Xiang Xinxin,Tian Wenxiu,Yang Baoye,Li Tao,Zhai Qiaoli
Osteoblast differentiation is a key process in bone homeostasis. Mutations in plastin 3 have been reported to be responsible for X-linked osteoporosis. Plastin 3 and plastin 2 act synergistically to regulate osteoblast differentiation. However, the bone-related function of plastin 1, another family member of plastins, has not been assessed. In this study, we addressed the functional importance of plastin 1 in osteoblasts. We characterized the expression patterns of plastin 1 during osteoblast differentiation and revealed its important role in this process. In both HEK 293T and hFOB1.19 cells, plastin 1 was demonstrated to regulate intracellular Ca2+. Accordingly, we revealed that higher Ca2+ concentration promotes osteoblast differentiation. Finally, we found that plastin 1 may play a compensatory role in osteoporosis patients with plastin 3 deficiency. Together, our results indicate that plastin 1 promotes osteoblast differentiation by regulating intracellular Ca2+. Our work sheds new light on the role played by plastins in bone homeostasis.
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CHEMICAL ENGINEERING JOURNAL
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2023
·
IF 15.1
Jian Guan,Xiangzeng Wang,Jingcheng Du,Quansheng Liang,Wen He,Ying Liu,Ji Ma,Chunwei Zhang,Jiangtao Liu
Polymers of intrinsic microporosity (PIMs) are promising candidates for carbon dioxide (CO 2 ) separation and capture from flue gas or natural gas. PIM-1, which has excellent gas permeation properties over the Robeson upper bound (1991), has been widely functionalized to further enhance the CO 2 permeability or selectivity. In this work, a simple and readily processive surface modification method has been proposed via hydrolysis of the PIM-1 membrane. The two hydrolysis processes by the acid method, amidation and carboxylation processes, have been conducted to functionalize the surface of PIM-1 membranes forming N A -PIM-1 and C A -PIM-1 membranes. The TGA, FT-IR spectra and NMR results verify that the composition and structure inside the PIM-1 membrane remain unchanged. XPS and NMR spectra demonstrate that the nitrile group at the surface was successfully functionalized with an amine group or carboxyl group. The CO 2 permeability and CO 2 /N 2 selectivity of the N A -PIM-1 membrane both increased to 4593.2 Barrer and 28.5, respectively. Another advantage of N A -PIM-1 membranes is the coordination site from introduced nitrogen atoms with zinc ions to form N A -PIM-1-Zn, which could further react with PEI to introduce amine-rich groups on the surface of the PIM-1 membrane. The CO 2 /N 2 selectivity of the N A -PIM-1-Zn-PEI membrane coated with PEI on the surface increases 45.4% compared to the unmodified PIM-1 membranes, with CO 2 permeability decreasing only 15.5% compared to the unmodified PIM-1 membrane. The N A -PIM-1 and N A -PIM-1-Zn-PEI membranes both surpass the Robeson upper bound (2008) even under mixed gas feeding, suggesting that surface modification of PIM-1 is a promising approach for CO 2 capture from natural gas and flue gas.
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International Journal of Electrochemical Science
·
2023
·
IF 1.5
Min Li,Ying Li
In this work, it found that lead oxide (PbO) can be dissolved well in 1-methylimidazolium trifluoromethylsulfonate (MIMTfO) ionic liquid. The electrodeposition of lead from PbO in MIMTfO ionic liquid was studied. Cyclic voltammetry studies showed that the reduction of Pb(II) is a irreversible and one-step reaction process. Chronoamperometry results presented that the nucleation mechanism of lead is fit instantaneous nucleation under diffusion control. The diffusion coefficient of Pb(II) at 373 K is approximately 3.6×10 –8 cm 2 s –1 . The electrodeposition experiments were conducted at 383 K and 393 K using potentiostatic electrolysis. A compact electrodeposit was obtained at 383 K in MIMTfO ionic liquid, which was confirmed to be metallic lead.
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Crystals
·
2019
·Vol. 9
·期 3
·130
·
IF 2.061
Lu Zhou,Xuefeng Chu,Yaodan Chi,Xiaotian Yang
In this study the effects of 1-Octadecanethiol (ODT, 1-CH3[CH2]17SH) passivation on GaAs (100) surface and GaAs/Al2O3MOS capacitors are investigated. The results measured by X-ray photoelectric spectroscopy (XPS), Raman spectroscopy and scan electron microscopy (SEM) show that the ODT passivation can obviously suppress the formation of As-O bonds and Ga-O bonds on the GaAs surface and produce good surface morphology at the same time, and especially provide better protection against environmental degradation for at least 24 h. The passivation time is optimized by photoluminescence (PL), and the maximum enhancement of PL intensity was 116%. Finally, electrical property of a lower leakage current was measured using the metal-oxide-semiconductor capacitor (MOSCAP) method. The results confirm the effectiveness of ODT passivation on GaAs (100) surface.Keywords:GaAs;passivation;XPS;RAMAN;PL
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2015
·Vol. 31
·期 2
·224-227
·
IF 0.875
Zhang Ran,Xu Lihua,Chen Hui,Qin Zhihong,Zhao Yu,Ni Zhonghai
1-Thiohydroxypyrene( 1 ) and its two intermediates, 1-pyrenyl- O -thiocarbamate( 2 ) and 1-pyrenyl- S -thiocarbamate( 3 ), were synthesized using 1-hydroxypyrene as the starting material. The key synthetic step is Newman-Kwart rearrangement. The results indicate that the Newman-Kwart rearrangement is more effective in suitable solvent than conventional method based on pure organic compound. The structures of compounds 1 – 3 were characterized by FTIR, NMR, GC-MS and elementary analysis. The crystal structures of two new compounds( 2 and 3 ) were determined by single crystal X-ray diffraction analysis. The whole synthetic process is simple, mild and with high yield.
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Reaction Chemistry & Engineering
·
2025
·
IF 3.1
Zhangxinyu Fan,Muhan Li,Yifan Sun,Weixiang Wang,Qin Zhong,Boyang Liu
Since its discovery by Otto Roelen, hydroformylation has attracted extensive attention for its ability to extend the carbon chain of olefins. Aldehydes, which are converted by the hydroformylation of olefins and syngas (CO/H2), are not only high-value products, but also intermediates to produce fine chemicals, such as alcohols, esters and amines. The currently used homogeneous catalysts bring about the loss of precious metals and the discharge of phosphorus-containing wastes. Therefore, heterogeneous catalysts are developed to simplify the separation process and enhance catalyst recovery. However, the reaction kinetics of heterogeneous hydroformylation, especially the hydroformylation of long-chain olefins, remain unclear. Unlike homogeneous hydroformylation, terminal olefins can be isomerized to internal olefins on heterogeneous catalysts, and can be further converted to different branched aldehydes. Thus, the reaction kinetic of heterogeneous hydroformylation is more complex. In this work, we established a kinetic model for the heterogeneous hydroformylation of long-chain terminal olefins on Rh-based phosphides, using 1-hexene as the model reactant. This kinetic model agrees well with the density functional theory (DFT) results, and can be used to predict the regioselectivity under different reaction conditions. This study reveals the kinetic mechanism of heterogeneous hydroformylation of long-chain terminal olefins, which paves the way for the rational design of heterogeneous catalysts and the theoretical optimization of reaction conditions.
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STAR Protocols
·
2024
·
IF 1.3
Shibo Sun,Haowen Liu,Wuyang Shi,Hanziyi Zhou,Hao Wu,Weiping Xu,Jianqiang Xu
Summary Selenoprotein thioredoxin reductase 1 (TXNRD1) is a promising therapeutic target, with several inhibitors reported to inhibit TXNRD1 activity. These inhibitors have the potential for applications such as anti-tumor medications. Here, we present a protocol for assessing irreversible inhibitors of TXNRD1. We describe four assays covering cellular TXNRD activity measurement, recombinant enzyme-based activity determination, differential scanning fluorimetry (DSF), and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. This protocol will facilitate the screening and development of potential small-molecule inhibitors of TXNRD1.
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PROGRESS IN ORGANIC COATINGS
·
2025
·
IF 7.3
Le Ma,Yang Hou,Jun Nie,Xiaoqun Zhu
The introduction of substituents affects the planarity of the molecule, resulting in different physical and chemical properties. This study synthesized four one-component visible light photoinitiators with different substituents derived from 1-naphthylamine. They were amine-free (BTX), primary amine (BTX-A), secondary amine (BTX-MA), and tertiary amine (BTX-DMA). The photophysical and photochemical properties were explored using UV–visible absorption spectroscopy combined with DFT theory computational simulations. They had excellent absorption properties in the visible light range ( ε : 3200–6300 L mol −1 cm −1 ). The structure of amine substitutions in the initiators showed different redshift phenomena. The maximum absorption wavelength of BTX in the visible region is 388 nm; only a 7 nm red shift occurred for the maximum absorption wavelength of BTX-DMA. While BTX-A had a maximum absorption wavelength of red shifting by 41 nm， the red shift for BTX-MA was about 53 nm, and its UV absorption tail extended to 520 nm. This phenomenon was known to be the result of the combined effect of electronic donor and molecular planarity according to the DFT theoretical calculations. The photopolymerization kinetics of four initiators used for double bond monomers was investigated by real-time FT-IR. The results indicated that polymerization could occur for all the designed systems; the highest double bond conversion could reach 92 % when BTX-DMA was used as a visible-light photoinitiator for the monomer of 2-hydroxyethyl acrylate (HEA).
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Reaction Chemistry & Engineering
·
2025
·
IF 3.1
Xueqian Zhang,Jiasong Yan,Shuai Yuan,Shanqing Yu,Qingyu Liu,Xikai Wang,Rui Li
Whether cycloalkanes undergo six- or five-membered ring-opening reaction pathways remains controversial. In this work, the catalytic cracking reactions of cyclohexene and 1-methylcyclopentene, as model compounds, were investigated in a pulse microreactor. The characteristics of the reactions of the two model compounds were compared in terms of isomerization, cracking, and hydrogen transfer reaction, and the reasons for the differences in their catalytic reactions were analyzed in combination with thermodynamic and molecular simulation calculations. The results have shown that the catalytic cracking product distributions of cyclohexene and 1-methylcyclopentene were similar owing to their easy mutual isomerization; the five-membered ring-opening cracking pathway was more thermodynamically advantageous than that of six-membered ring-opening cracking, and therefore, the yield of the ring-opening cracking product of 1-methylcyclopentene was comparatively higher; the main products of the hydrogen transfer reaction of the two compounds were alkanes and aromatic hydrocarbons. By comparing the product distributions and reaction activation energies, the ring-opening reaction pathways were clarified, and the catalytic cracking reaction networks of cyclohexene and 1-methylcyclopentene were established.
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MACROMOLECULAR RAPID COMMUNICATIONS
·
2023
·Vol. 44
·期 17
·2300166
·
IF 4.6
Shuai Tang,Jiao Li,Zhao Wang,Liqun Zhang
Polarity determines the oil resistance property of elastomers. In this work, three bio-based polyester elastomers (BPEs) with different mass fraction of ester groups ( E ) are designed and synthesized aiming to study the relationship of E and oil resistance performance, and to obtain bio-based elastomer materials with tunable oil resistance. Through adjusting the chain length of monomers, E of poly(ethylene glycol/1,3-propanediol/succinate/adipate/itaconate)(PEPSAI), poly(1,3-propanediol/1,4-butanediol/succinate/adipate/itaconate)(PPBSAI), and poly(1,3-propanediol/1,4-butanediol/sebacate/adipate/itaconate)(PPBSeAI) are ≈50.39%, 48.55%, and 39.68%, respectively. Results show that E has great influence on the oil resistance of BPEs. After being immersed in IRM-903 # oil for 72 h at room temperature, the changes in mass and volume of BPEs decrease along with the increasing mass fraction of ester groups, indicating improved oil resistance performance. PEPSAI with the highest mass fraction of ester groups presents better oil resistance and lower T g (better low-temperature resistance) than one of the most used commercial oil-resistant rubber nitrile rubber (N230S). Thus, this work provides a promising strategy to obtain bio-based oil resistant elastomers with practical value.
研究领域:
材料科学
·
高分子化学
·
生物技术
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Renjie Zhou,Cheng Yang,Ting Xie,Jian Zhang,Chenqiang Wang,Ziqiang Ma,Lianfu Zhang
Amadori compounds (ACs) are the first stable product formed in the initial stage of the Maillard reaction and are widely present in processed foods. In addition to being widely known as the flavor precursors, ACs have attracted attention in recent years because many functions have been revealed, including ACE inhibition. In this work, we synthesized four ACs ( N -(1-Deoxy-D-fructos-1-yl)-glycine (Fru-Gly), N -(1-Deoxy-D-fructos-1-yl)-proline (Fru-Pro), N -(1-Deoxy-D-fructos-1-yl)-serine (Fru-Ser) and N -(1-Deoxy-D-fructos-1-yl)-threonine (Fru-Thr)) and characterized them using UPLC-Q-TOF-MS spectra. The four ACs’ angiotensin-converting enzyme (ACE) inhibitory activities were verified for the first time, the IC 50 value range was 1.447–4.204 mmol/L, the Fru-Gly has the best effect. And all the four ACs showed good digestion stability, the content remained above 70% after 5 h of continuous digestion simulation. In order to reveal the mechanism of ACs inhibiting ACE, we selected Fru-Gly, beacuse of the best inhibitory effect, to carried out enzyme kinetics, circular dichroism spectroscopy, fluorescence spectroscopy, thermodynamics and molecular docking analysis. The results showed that Fru-Gly was a noncompetitive inhibitor, it spontaneously binds to the inactive site of ACE by hydrogen bond and causes the conformation change of ACE to inactivate it. The reaction is easier to proceed at low temperatures.
研究领域:
食品科学
·
生物化学
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2013
·
IF 2.417
Jian Xu,Yan-Yan Jia,Shao-Rui Chen,Jian-Tao Ye,Xian-Zhang Bu,Yun Hu,Yun-Zi Ma,Jin-Lei Guo,Pei-Qing Liu
Flavonoids are a class of compounds that exist in nature with the structure of 2-phenyl-chromone. In Chinese traditional medicine , herbal drugs containing flavonoids are widely used for the treatment of inflammation, cardiovascular disease, tumor and so on. In this study, we investigated the anti-inflammatory effect and related mechanisms of a novel synthetic flavonoid, (E)-1-(4-ethoxyphenyl)-3-(4-nitrophenyl)-prop-2-en-1-one (ETH) in lipopolysaccharide (LPS) stimulated macrophages. Our results showed that ETH inhibited LPS-induced TNF-α and IL-6 release in a dose-dependent manner, and decreased TNF-α, IL-1β, IL-6 and iNOS mRNA production. LPS-induced expression of cyclooxygenase-2 (COX-2) was also significantly attenuated by ETH. Pretreatment with ETH reduced the I-κBα phosphorylation, p65 nuclear translocation as well as NF-κB-dependent transcriptional activity. In addition, ETH exhibited a significant protection against LPS-induced inflammatory mortality in mice. Taken together, these findings suggest that ETH can inhibit LPS-induced inflammation via suppressing NF-κB signaling pathway , indicating that ETH may be a potential anti-inflammatory agent.
研究领域:
药理学
·
免疫学
·
医学
·
生物化学
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