Science China-Chemistry
·
2022
·Vol. 65
·期 10
·2038-2044
·
IF 10.138
Wang Yanan,Pei Chunlei,Zhao Zhi-Jian,Gong Jinlong
Rational design and performance promotion are eternal topics and ultimate goals in catalyst preparation. In contrast, trial—and—error is still the common method people take. Therefore, it is important to develop methods to intrinsically enhance the performance of catalysts. The most effective solutions are the one from a kinetic perspective based on clear knowledge of the reaction mechanism. This paper describes rate-determining step cognition and modulation to promote CO oxidation on highly dispersed Pt on CeO 2 . The different degrees of metal—support interactions due to variation of hydroxyl density of support could alter the structure of active species and the ability of oxygen activation apparently, further shift the rate-determining step from oxygen activation to oxygen reverse spillover kinetically. The transformation of rate-determining step could enhance the intrinsic activity significantly, and decrease the T 50 approximately 140 °C. The findings of this research exemplify the universal and effective method of performance elevation by rate-determining step modulation, which is promising for application in different systems.
研究领域:
催化
·
化学工程
·
材料科学
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DOI
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2023
·Vol. 21
·期 1
·1-24
·
IF 10.2
Liu Ziyao,Chen Bei,Xiang Shijun,Hu Shuo
Background Pharmacotherapy constitutes the first-line treatment for depression. However, its clinical use is hindered by several limitations, such as time lag, side effects, and narrow therapeutic windows. Nanotechnology can be employed to shorten the onset time by ensuring permeation across the blood brain barrier (BBB) to precisely deliver more therapeutic agents; unfortunately, formidable challenges owing to the intrinsic shortcomings of commercial drugs remain.Results Based on the extraordinary capability of monoamines to regulate the neuronal environment, we engineer a network nanocapsule for delivering serotonin (5-hydroxytryptamine, 5-HT) and catalase (CAT) to the brain parenchyma for synergistic antidepression therapy. The nanoantidepressants are fabricated by the formation of 5-HT polymerization and simultaneous payload CAT, following by surface modifications using human serum albumin and rabies virus glycoprotein. The virus-inspired nanocapsules benefit from the surface-modifying strategies and exhibit pronounced BBB penetration. Once nanocapsules reach the brain parenchyma, the mildly acidic conditions trigger the release of 5-HT from the sacrificial nanocapsule. Releasing 5-HT further positively regulate moods, relieving depressive symptoms. Meanwhile, cargo CAT alleviates neuroinflammation and enhances therapeutic efficacy of 5-HT.Conclusion Altogether, the results offer detailed information encouraging the rational designing of nanoantidepressants and highlighting the potential of nanotechnology in mental health disorder therapies.Graphical abstract
研究领域:
药理学
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纳米医学
·
神经科学
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心理健康
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DOI
PubMed
高影响因子
Nanoscale Research Letters
·
2014
·Vol. 9
·期 1
·1-8
·
IF 2.481
Liu Zhong-Tao,Xiong Li,Liu Zhi-Peng,Miao Xiong-Ying,Lin Liang-Wu,Wen Yu
This study aimed to compare the inhibitory effects of photosensitizers loaded in hollow silica nanoparticles and conventional photosensitizers on HepG2 human hepatoma cell proliferation and determine the underlying mechanisms. Photosensitizers (conventional Photosan-II or nanoscale Photosan-II) were administered to in vitro cultured HepG2 hepatoma cells and treated by photodynamic therapy (PDT) with various levels of light exposure. To assess photosensitizers' effects, cell viability was determined by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In addition, apoptotic and necrotic cells were measured by flow cytometry and the expression of caspase-3 and caspase-9 evaluated by western blot. Finally, the in vivo effects of nanoscale and conventional photosensitizers on liver cancer were assessed in nude mice. Nanoscale Photosan-II significantly inhibited hepatoma cell viability in a concentration-dependent manner and this effect was more pronounced with high laser doses. Moreover, nanoscale photosensitizers performed better than the conventional ones under the same experimental conditions (p < 0.05). Flow cytometry data demonstrated that laser-induced cell death was markedly increased after treatment with nanoscale Photosan-II in comparison with free Photosan-II (p < 0.05). Activated caspase-3 and caspase-9 levels were significantly higher in cells treated with Photosan-II loaded in silica nanoparticles than free Photosan-II (p < 0.05). Accordingly, treatment with nanoscale photosensitizers resulted in improved outcomes (tumor volume) in a mouse model of liver cancer, in comparison with conventional photosensitizers. Hollow silica nanoparticles containing photosensitizer more efficiently inhibited hepatoma cells than photosensitizer alone, through induction of apoptosis, both in vivo and in vitro.
研究领域:
肿瘤学
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纳米技术
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药理学
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DOI
PubMed
高影响因子
Nano-Micro Letters
·
2020
·Vol. 12
·期 1
·1-15
·
IF 12.264
Miao Yalei,Qiu Yudian,Zhang Mengna,Yan Ke,Zhang Panke,Lu Siyu,Liu Zhongyi,Shi Xiaojing,Zhao Xubo
AbstractSection
Highlights
The formation of manganese oxide induces self-assembly of block copolymers to form polymeric vesicles.
The polymeric vesicles possessed strong stability and high drug loading capacity.
The drug-loaded polymeric vesicles have been demonstrated, especially in in vivo studies, to exhibit a higher efficacy of tumor suppression without known cardiotoxicity.
AbstractSection
Abstract
Molecular self-assembly is crucially fundamental to nature. However, the aqueous self-assembly of polymers is still a challenge. To achieve self-assembly of block copolymers [(polyacrylic acid– block –polyethylene glycol– block –polyacrylic acid (PAA 68 – b –PEG 86 – b –PAA 68 )] in an aqueous phase, manganese oxide (MnO 2 ) is first generated to drive phase separation of the PAA block to form the PAA 68 – b –PEG 86 – b –PAA 68 /MnO 2 polymeric assembly that exhibits a stable structure in a physiological medium. The polymeric assembly exhibits vesicular morphology with a diameter of approximately 30 nm and high doxorubicin (DOX) loading capacity of approximately 94%. The transformation from MnO 2 to Mn 2+ caused by endogenous glutathione (GSH) facilitates the disassembly of PAA 68 – b –PEG 86 – b –PAA 68 /MnO 2 to enable its drug delivery at the tumor sites. The toxicity of DOX-loaded PAA 68 – b –PEG 86 – b –PAA 68 /MnO 2 to tumor cells has been verified in vitro and in vivo. Notably, drug-loaded polymeric vesicles have been demonstrated, especially in in vivo studies, to overcome the cardiotoxicity of DOX. We expect this work to encourage the potential application of polymer self-assembly.
研究领域:
材料科学
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药学
·
生物医学工程
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纳米技术
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DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2017
·Vol. 53
·期 3
·1623-1633
·
IF 2.599
Qiao Yujie,Liu Xuelong,Fu Gongbu,He Zhongyuan,Hou Chengyi,Li Yaogang,Zhang Qinghong,Yan Hexin,Wang Hongzhi
Nanofiber scaffolds are widely used as the platform for three-dimensional culture of hepatocytes in vitro. The pore size of scaffolds plays an important role in promoting the infiltration and proliferation of hepatocyte. We show that the average pore size of electrospun scaffold increases from ~ 7.6 to 13.2 μm, while the average fiber diameter decreases from ~ 2.0 to 1.5 μm when collected by probe array collectors. Though increase in pore size decreases the tensile stress of scaffolds, it leads to enhance the proliferation and attachment of hepatocytes. Specifically, a 6 × 6 array scaffold which was prepared by probe collector was orderly arrayed. Compared with the conventional scaffold, the pore size of the arrayed scaffold doubled and the hydrophilicity was improved. When HepG 2 cells were seeded on the arrayed scaffold, cells showed superior adhesion ability, better cell morphology and three-dimensional growth. These results indicated that the ordered 6 × 6 array scaffold has the potential as a suitable substratum for in vitro culture of hepatocytes.
研究领域:
生物材料
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组织工程
·
细胞生物学
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DOI
高影响因子
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
·
2021
·Vol. 53
·期 3
·275-284
·
IF 2.945
Yang Rong,Li Xue,Mei Jie,Wan Wencheng,Huang Xinduo,Yang Qiaohong,Wei Xiaoyong
We have previously reported that syringic acid (SA) extracted from D. aurantiacum var. denneanum (kerr) may be used to prevent diabetic cataract (DC). However, the underlying mechanisms through which SA prevents DC in human lens epithelial cells (HLECs) remained unclear. In the present study, we employed single-molecule optics technologies, including transmission electron microscopy (TEM), atomic force microscopy (AFM), laser scanning confocal microscopy (LSCM) and Raman spectroscopy, to monitor the effect of SA on HLECs biomechanics and organelle structure in real-time. TEM suggested that SA improved the ultrastructure of HLECs with regard to nuclear chromatin condensation and reducing mitochondrial swelling and degeneration, which may aid in the maintenance of HLECs integrity in the presence of glucose. AFM revealed a reduced surface roughness and stiffness following SA treatment, suggesting an improved viscoelasticity of HELCs. Raman spectrometry and LSCM further revealed that these changes were related to a modification of cell liquidity and cytoskeletal structure by SA. Taken together, these results provide insights into the effects of SA on the biomechanics of HLECs and further strengthen the evidence for its potential use as a novel therapeutic strategy for DC prevention.
研究领域:
药理学
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细胞生物学
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眼科
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DOI
PubMed
高影响因子
TRANSACTIONS OF TIANJIN UNIVERSITY
·
2020
·Vol. 27
·期 1
·77-86
·
IF 0
Zhai Yuanyuan,Qu Hongmei,Li Zhongxuan,Zhang Bo,Cheng Jinxi,Zhang Jiaji
In this study, we modified microcrystalline cellulose by cross-linking it with epichlorohydrin to obtain a rapid and efficient adsorbent for the removal of Reactive Blue 4 dye from aqueous solution. Evidences of the cross-linking of the microcrystalline cellulose were obtained by Fourier transform infrared spectroscopy, X-ray diffraction, Brunauer–Emmett–Teller analysis, thermogravimetric analysis, and scanning electron microscopy. We investigated the effects of adsorbent dosage, pH, initial dye concentration, temperature, and contact time on the dye adsorption capacity. The results showed that the adsorption equilibrium time was just 20 min and the maximum adsorption capacity was 69.79 mg/g. The adsorption isotherm data fitted the Langmuir isotherm model well, and the adsorption kinetics data followed the pseudo-second-order kinetic model. The results of the thermodynamic analysis suggest that the adsorption process was spontaneous and exothermic. Recyclability experiments demonstrated the good reusability of this adsorbent. Electrostatic interaction was found to dominate the adsorption process.
研究领域:
环境科学
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化学工程
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材料科学
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DOI
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 2
·1-9
·
IF 5.479
Zheng Jiushang,Wang Bin,Jin Yanzi,Weng Bo,Chen Jiucun
A novel MXene-based biomimetic enzyme was synthesized using adenosine triphosphate (ATP) as a template to modify a Mn 3 (PO 4 ) 2 nanostructure on Mxene-Ti 3 C 2 nanosheets. The resulting composite was used as an electrode material in an electrochemical sensor for superoxide anion (O 2 •− ). It displays excellent catalytic properties which is attributed to the synergistic effects of the two-dimensional conductive substrate and the Mn 3 (PO 4 ) 2 nanoparticles. The addition of ATP results in the formation of a porous and ordered nanostructure of Mn 3 (PO 4 ) 2 . This facilitates the electron transfer between O 2 •− and electrode. The sensor, best operated at 0.75 V (vs. Ag/AgCl), displays a rapid amperometric response with a detection limit of 0.5 nM and an analytical range that extends from 2.5 nM to 14 μM. Conceivably, it has potential in the detection of O 2 •− released by living cells.
Nanostructured MXenes were synthesized by in-situ growth of Mn 3 (PO 4 ) 2 on Ti 3 C 2 nanosheets under the induction of adenosine triphosphate (ATP). They display enzyme mimickong properties. A sensor fabricated with the composites can be used for the detection of superoxide anions released by HepG2 cells.
研究领域:
材料科学
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电化学
·
生物传感器
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纳米技术
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DOI
PubMed
高影响因子
Waste and Biomass Valorization
·
2019
·Vol. 11
·期 8
·3987-4002
·
IF 2.358
Rakariyatham Kanyasiri,Liu Xiaoyang,Liu Zhongyuan,Wu Sufeng,Shahidi Fereidoon,Zhou Dayong,Zhu Beiwei
Abstract This study demonstrated increases in the recovery of phenolic compounds from industrial waste, longan peel, after treatment with plant matrix degrading enzymes. Pretreatment of longan peel with cellulase, α-amylase, protease, or β-glucosidase enhanced total phenolic content in the extracts, leading to increases of DPPH scavenging activity, oxygen radical absorbance capacity, antioxidant in β-carotene/linoleate system, and ferrous ion chelating activity. In detail, cellulase increased the release of free phenolics e.g. o -coumaric acid, corilagin, and quercetin as well as the release of esterified phenolics e.g. ellagic acid, and gallic acid, while β-glucosidase increased the release of free phenolics e.g. ellagic acid. Among all enzymatic pretreatments, protease provided the highest proportion of phenolics’ recovery in the extract, while only cellulase and β-glucosidase significantly improved extraction yields by 31 and 17%, respectively. These results indicated enhancement of valorization of longan peel as a resource material for functional ingredients recovery. Graphic Abstract
研究领域:
食品科学
·
植物化学
·
生物技术
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DOI
高影响因子
Nature Communications
·
2021
·Vol. 12
·期 1
·1-15
·
IF 14.919
Shi Tonglei,Hou Xuan,Guo Shuqing,Zhang Lei,Wei Changhong,Peng Ting,Hu Xiangang
Biofilms contribute to bacterial infection and drug resistance and are a serious threat to global human health. Antibacterial nanomaterials have attracted considerable attention, but the inhibition of biofilms remains a major challenge. Herein, we propose a nanohole-boosted electron transport (NBET) antibiofilm concept. Unlike known antibacterial mechanisms (e.g., reactive oxygen species production and cell membrane damage), nanoholes with atomic vacancies and biofilms serve as electronic donors and receptors, respectively, and thus boost the high electron transport capacity between nanomaterials and biofilms. Electron transport effectively destroys the critical components (proteins, intercellularly adhered polysaccharides and extracellular DNA) of biofilms, and the nanoholes also significantly downregulate the expression of genes related to biofilm formation. The anti-infection capacity is thoroughly verified both in vitro (human cells) and in vivo (rat ocular and mouse intestinal infection models), and the nanohole-enabled nanomaterials are found to be highly biocompatible. Importantly, compared with typical antibiotics, nanomaterials are nonresistant and thereby exhibit high potential for use in various applications. As a proof-of-principle demonstration, these findings hold promise for the use of NBET in treatments for pathogenic bacterial infection and antibiotic drug resistance.
研究领域:
纳米技术
·
微生物学
·
生物医学工程
·
材料科学
·
药学
✓ 已验证
DOI
PubMed
高影响因子
GLYCOCONJUGATE JOURNAL
·
2022
·Vol. 39
·期 4
·475-486
·
IF 3.009
Zhai Xiaona,Zhang Weida,Pei Haisheng,Chen Guogang
Poria cocos, a famous traditional Chinese medicine and a well-known food or food supplement, has shown therapeutic potential against cancer and the uneasiness of the mind. In addition, polysaccharides (PCPs) in this fungus were found to be various bioactive. In this work, one such PCP, PCP-1, extracted by deep eutectic solvent (DES) and separated using Sephadex G-15 columns, was characterized using GC–MS, HPGPC, FT-IR, and NMR, while also tested for physicochemical properties. Results indicated that PCP-1 contained 96.89 ± 3.21% total sugars and was a glucan with molecular weight of 3.2 kD. The main glycosidic linkage was 1,3-linked Glc p with 96.82 mol% content and a triple helix structure, and β-D-Glc p -(1 → linkage connected to the main chain through an O-6 atom was the backbone structure. In terms of the physicochemical property, PCP-1 was soluble in water, but not in organic solvent, and processed a relative high water-holding capacity (8.64 ± 0.14 g/g) and low oil-holding capacity (2.52 ± 0.21 g/g). In addition, in vitro , PCP-1 was found to have the ability of scavenging DPPH, hydroxyl free radical, superoxide anion radical and reducing ferric at different levels. This research would be useful for the further application of PCP-1.
研究领域:
药学
·
食品科学
·
生物技术
·
分析化学
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DOI
PubMed
高影响因子
Particle and Fibre Toxicology
·
2021
·Vol. 18
·期 1
·1-17
·
IF 9.4
Yue Tongtao,Lv Rujie,Xu Dongfang,Xu Yan,Liu Lu,Dai Yanhui,Zhao Jian,Xing Baoshan
Background Airborne nanoparticles can be inhaled and deposit in human alveoli, where pulmonary surfactant (PS) molecules lining at the alveolar air–water interface act as the first barrier against inhaled nanoparticles entering the body. Although considerable efforts have been devoted to elucidate the mechanisms underlying nanoparticle-PS interactions, our understanding on this important issue is limited due to the high complexity of the atmosphere, in which nanoparticles are believed to experience transformations that remarkably change the nanoparticles’ surface properties and states. By contrast with bare nanoparticles that have been extensively studied, relatively little is known about the interactions between PS and inhaled nanoparticles which already adsorb contaminants. In this combined experimental and computational effort, we investigate the joint interactions between PS and graphene-family materials (GFMs) with coexisting benzo[a]pyrene (BaP). Results Depending on the BaP concentration, molecular agglomeration, and graphene oxidation, different nanocomposite structures are formed via BaPs adsorption on GFMs. Upon deposition of GFMs carrying BaPs at the pulmonary surfactant (PS) layer, competition and cooperation of interactions between different components determines the interfacial processes including BaP solubilization, GFM translocation and PS perturbation. Importantly, BaPs adsorbed on GFMs are solubilized to increase BaP’s bioavailability. By contrast with graphene adhering on the PS layer to release part of adsorbed BaPs, more BaPs are released from graphene oxide, which induces a hydrophilic pore in the PS layer and shows adverse effect on the PS biophysical function. Translocation of graphene across the PS layer is facilitated by BaP adsorption through segregating it from contact with PS, while translocation of graphene oxide is suppressed by BaP adsorption due to the increase of surface hydrophobicity. Graphene extracts PS molecules from the layer, and the resultant PS depletion declines with graphene oxidation and BaP adsorption. Conclusion GFMs showed high adsorption capacity towards BaPs to form nanocomposites. Upon deposition of GFMs carrying BaPs at the alveolar air–water interface covered by a thin PS layer, the interactions of GFM-PS, GFM-BaP and BaP-PS determined the interfacial processes of BaP solubilization, GFM translocation and PS perturbation.
研究领域:
纳米技术
·
材料科学
·
生物物理学
·
环境科学
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
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