TALANTA
·
2023
·
IF 6.1
Yilu Wang,Qiming Chen,Yingying Wang,Fangmin Tu,Xiaodi Chen,Junhai Li,Zhanmin Liu
Helicobacter pylori ( H. pylori ) is a kind of microaerobic and food-borne pathogen. More than 4.4 billion individuals have been infected by H. pylori and H. pylori was listed as Group I carcinogen by WHO in 1994. Considering the high infection rate of H. pylori and the limited medical resources, self-testing is helpful for diagnosis and timely treatment. Although the amount of H. pylori in human saliva is low, the sampling of saliva is simple and convenient compared with stomach, blood and stool samples. Therefore, H. pylori in human saliva can be an indicator for self-testing, and a sensitive and easy-to-use assay is necessary. In this study, we developed a time-resolved fluorescent microsphere-lateral flow immunoassay (TRFM-LFIA) strip assay with image visual analysis for detection of H. pylori in saliva. The detection of the TRFM-LFIA strip was easy to use and had a low dependency on equipment. With optimized preparation and detection parameters, the whole detection process could be finished in 8 min and the LOD by naked eyes was 10 2 CFU/mL. For quantitative analysis by image visual analysis, the LOD was as low as 1.05 CFU/mL in the linear range of 10 1 –10 5 CFU/mL. Besides, the TRFM-LFIA strip also had good stability, reliability, repeatability and accuracy. All these advantages indicated that the TRFM-LFIA strips developed in this study had a good potential for self-testing for H. pylori infection.
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ANALYTICAL LETTERS
·
2023
·
IF 2
Hongdan Wang,Wei Yuan,Liqiu Zhang,Baogang Xie,Qiong Cheng,Ningwen Zhang
To precisely detect CA199 antigen, a novel electrochemical immunosensor based on horseradish peroxidase (HRP) and glucose oxidase (GOD) double enzyme system was prepared. Thiol was adsorbed on the surface of nano-octahedral gold through Au-S self-assembly; the second antibody was labeled by glutaraldehyde ligase as a signal amplification reagent. Amino magnetic microspheres were used to connect GOD and bovine serum albumin (BSA) through glutaraldehyde. The biosensor displayed low detection limits of 0.2 U/mL and wide linear range (0.5–200 U/mL). This novel immunosensor improves the sensitivity of current detection, showing promising application prospects in the clinical immunoassay detection of CA199.
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CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Junfeng Yu,Nanxi Dong,Bingxue Liu,Guofeng Tian,Shengli Qi,Dezhen Wu
Polymer separators with high-temp dimensional stability is strongly demanded for high-safety lithium-ion batteries (LIBs). In this study, a novel polyimide (PI) microsphere coating slurry was prepared and then plated on Celgard polypropylene (PP) separator, producing PP@PI microsphere composite separator with excellent thermal stability, flame retardancy, high liquid retention capacity and high ionic conductivity by a simple blade-coating process. The results show that the thermal stability and liquid retention capacity of the PP@PI microsphere composite separator are significantly improved. Compared with the pure commercial PP separator which sharply shrank at 150 °C, the PI microsphere composite separator shows negligible shrinkage over 150 °C. The obtained PP@PI microsphere composite separators exhibited excellent thermal stability and fire-resistance properties. At the same time, the peel strength of composite separator reached 138.6 N·m −1 , with excellent adhesion. The surface density of PP@PI microsphere composite separator is 16.62 g·m −2 , which is lower than 30.22 g·m −2 of ceramic coated composite separator. Electrochemical characterizations indicate that the discharge capacity and capacity retention rate of the battery assembled with PP@PI microsphere composite separator are significantly higher than those assembled with the pure PP separator. The half-cell assembled with this composite separator shows a high capacity of 144.3 mAh·g −1 at 5C, which is much higher than the 117.8 mAh·g −1 of the commercial PP separator equipped battery. Moreover, the lithium-ion batteries assembled with the PP@PI microsphere composite separator show excellent cycle stability after 200 cycles at 1C. More importantly, the lithium-ion battery assembled with PP@PI microsphere composite separator has excellent safety at high temperature. These features demonstrate the potential of PP@PI microspheres composite membrane as high-security separator for LIBs.
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Journal of Environmental Chemical Engineering
·
2021
·
IF 5.909
Yanqing Cong,Wanxing Wang,Xiang Chen,Shi-Wen Lv
Nowadays efficient treatment of emerging organic contaminants is still a complex task with a big challenge. Herein, a novel dandelion-like NiCo 2 O 4 microsphere was synthesized by a simple step-by-step self-assembly strategy, and obtained NiCo 2 O 4 was then as an efficient heterogeneous catalyst to activate PS for rapid degradation of emerging organic pollutants . Importantly, as-prepared NiCo 2 O 4 -300 microsphere not only exhibited great catalytic activity , but also possessed excellent performance stability and structural stability. More significantly, developed NiCo 2 O 4 -300/PS oxidation system displayed strong degradation ability toward typical contaminants, including TC , IBP, SMX, BPA , DFC as well as OFX. TOC analyses revealed that these organic pollutants could eventually be degraded into H 2 O, CO 2 and other byproducts, and wherein degradation byproducts were low toxicity or no toxicity. During the process of PS activation over NiCo 2 O 4 -300, the redox couples of Co 3+ /Co 2+ and Ni 3+ /Ni 2+ provided major catalytic sites, and the redox couples of Ni 3+ /Ni 2+ played a bigger role. Furthermore, it was also found that both SO 4 −• and •OH were main active species in the reaction system. Even in actual water samples, proposed NiCo 2 O 4 -300/PS oxidation system still showed great performance for removing pollutants. In short, current work would provide valuable references for further research.
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JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2021
·
IF 6.064
Jian He,Xulin Hu,Luyao Xing,Dongliang Chen,Lijun Peng,Gaofeng Liang,Chengdong Xiong,Xiangchun Zhang,Lifang Zhang
Tissue-engineered scaffolds acted as active natural extracellular matrices that allowed for cell attachment, migration, proliferation, and differentiation. To enhance the drug loading, degradation control, and mechanical features of tissue-engineered scaffolds used in bone regeneration applications, we synthesized novel poly(trimethylene carbonate) (PTMC)/modified HA nanoparticles (KHA NPs, modified by silane coupling agent)/vancomycin hydrochloride (VH) porous microspheres scaffold. The fabricated PTMC/KHA/VH scaffold has unique surface corrosion degradation, excellent surface properties, and high cytocompatibility. The novel PTMC/KHA/VH scaffold was characterized by 42% porosity, 100 μm pore size, and 208.2 MPa compressive modulus. After the treatment of a rat femur defect for 4, 8, and 12 weeks, the surface corrosion biodegraded of the PTMC/KHA/VH scaffold remarkably decreased inflammation response and promoted bone regeneration, suggesting its use as a bioactive structure in bone tissue engineering.
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Optical Materials Express
·
2021
·Vol. 11
·期 7
·2076-2088
·
IF 3.442
Chen Junfan,Chen Hongxian,Zhang Jie,Zhu Yong
Combining self-assembly technology and the thin-film annealing method, annealed Ag nano-islands (Ag NIs) with SiO2 microsphere (MS) arrays were prepared as surface-enhanced Raman scattering (SERS) substrate in this paper. A “top-binding” model was established to analyze the effect of SiO2 MS at different annealing temperatures and different Ag film thickness. The corresponding comparative analysis with and without MS templates have been carried on. We simulated the electric field enhancement, coupling enhancement spectrum and the optical focus function of SiO2 MS. Characterization and experiments showed that the use of a SiO2 MS array as a dewetting template can well regulate the size and morphology of Ag NIs. Among them, SiO2 MS-Ag-20 can achieve the most uniform particle size at an annealing temperature of 873.15 K. According to the UV-Vis absorption spectrum, it had an active SERS analytical enhancement factor (AEF) of ∼3.91×105 and good SERS reproducibility (relative standard deviation, RSD = 0.113) for R6G when the wavelength was 532 nm.
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ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Yonglei Wu,Haizhu Lin,Zixing Xu,Yan Li,Zongbao Chen,Chunhui Deng
In this work, for the first time, L -cysteine ( L -Cys), a zwitterionic hydrophilic compound containing abundant amino and carboxyl was modified on silica microspheres for the fabrication of SiO 2 @ L -Cys capillary packed column. Since the SiO 2 @ L -Cys capillary packed column possessed great hydrophilicity brought by L -cysteine, they could be utilized to enrich glycopeptides with a low detection limit of 5 fmol μL −1 HRP digests as well as a good selectivity of the mixture of HRP and BSA digests up to a molar ratio of 1:50. The capillary packed column were further applied into the glycosylation analysis of human saliva and 69 glycopeptides were identified from 2 μL human saliva digests.
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ELECTROCHIMICA ACTA
·
2019
·
IF 5.383
Rajendran Ramachandran,Changhui Zhao,Muniyandi Rajkumar,Krishnamoorthy Rajavel,Pengli Zhu,Wenlu Xuan,Zong-Xiang Xu,Fei Wang
The porous structure of three-dimensional NiO microspheres on titanium carbide (NiO/Ti 3 C 2 T x ) is prepared by calcination of Ni-MOF/Ti 3 C 2 T x in the air. The crystalline structure and morphology of the obtained hybrid are characterized with various tools such as X-ray photoelectron spectroscopy and X-ray diffraction, scanning electron microscope, transmission electron microscope, and Brunauer-Emmett-Teller surface analyzer techniques. As-prepared NiO/Ti 3 C 2 T x hybrid is used for two noteworthy applications in electrochemistry like supercapacitor and non-enzymatic hydrogen peroxide (H 2 O 2 ) sensor. NiO/Ti 3 C 2 T x electrode exhibited an enhanced specific capacity of 630.9C g −1 at a current density of 1 A g −1 in comparison to pure NiO (376.8C g −1 ). Furthermore, the H 2 O 2 sensing performance of the NiO/Ti 3 C 2 T x modified glassy carbon electrode is evaluated in 0.5 M of NaOH solution and the electrode showed a low detection limit of 0.34 μM with a wider range of linear response 10 μM to 4.5 mM. The higher specific surface area and porosity of NiO/Ti 3 C 2 T x allow more electro-active site for electrochemical redox reactions in the direction of H 2 O 2 sensing and supercapacitor. Moreover, Ti 3 C 2 T x prevents from fouling in 3D porous network and leaching effect, and beneficial for easy access of electrolyte ions and efficient electron transport to the electrode surface resulted in improved electrochemical applications.
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JOURNAL OF HAZARDOUS MATERIALS
·
2019
·
IF 7.65
Pengfei Qi,Rong Luo,Thomas Pichler,Jianqiang Zeng,Yan Wang,Yuhua Fan,Kunyan Sui
Removal of trivalent species of As and Sb from wastewater is crucial due to their more toxic and mobile properties. In this study, a novel magnetic core-shell microsphere Fe 3 O 4 @TA@UiO-66 was developed via in-situ crystal growth of UiO-66 around the magnetic Fe 3 O 4 modified by Tannic Acid (TA). Characterization of the microsphere by transmission electron microscopy (TEM) and X-ray diffraction spectroscopy (XRD) confirmed that UiO-66 was adhered on the surface of Fe 3 O 4 functionalized by TA. Adsorption experiments showed that the magnetic Fe 3 O 4 @TA@UiO-66 had high adsorption capacity for As(III) and Sb(III) and could be rapidly separated from aqueous media within two minutes after treatment. The adsorption kinetics and adsorption isotherms were described well by the pesudo-second order model and Langmuir model, respectively. In addition, the composite exhibited excellent removal performance for As(III) and Sb(III) in a broad solution chemistry environment, including pH and co-existing anions. Based on X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR) measurement, we proposed that the removal mechanism was mainly controlled through a synergistic interaction of surface complexation and hydrogen bonding. This study indicates the potential of the magnetic microsphere to be used as an effective material for the removal of As(III) and Sb(III) from water.
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POLYMERS FOR ADVANCED TECHNOLOGIES
·
2018
·Vol. 29
·期 10
·2665-2673
·
IF 2.137
Xiaodong Guo,Xin Wang,Xiongrui Liu,Yanyang Zheng,Jianzhong Xu,Haiyun Ma
A phosphazene derivative flame retardant with a highly cross-linked microsphere structure, named poly(cyclotriphosphazene-c-sulfonyldiphenol) (PCPS) microspheres, were synthesized by 1-pot reaction and then applied on flame retarded epoxy (EP) resin. The microstructure and chemical composition of PCPS microspheres were characterized using scanning electron microscopy, transmission electron microscopy, and element mapping. The thermal stability of PCPS microspheres and PCPS/EP composites was explored through thermogravimetric analysis. Thermogravimetric data showed that the PCPS microspheres have excellent thermal stability, and the char yield is about 43% at the end of 800°C. The incorporation of PCPS microspheres significantly increased the char yield of PCPS/EP composites. The flammability was investigated by limited oxygen index tests and cone calorimeter. The limited oxygen index value of PCPS/EP composite was increased to 29.8 from 26.6 when 3 wt% of PCPS microspheres was added. Compared with neat EP, the flame retardancy was greatly improved. The peak heat release rate and smoke production rate of PCPS/EP composites were reduced by 45.0% and 43.6%, respectively. The mechanical properties including tensile strength and modulus were both improved due to the enhancement of PCPS microspheres. The PCPS microspheres act as a dual function for improving both the flame resistance and mechanical strength of PCPS/EP system.
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APPLIED SURFACE SCIENCE
·
2017
·
IF 3.387
Xiang Chen,Haibing Xu,Dong Liu,Chun Yan,Yingdan Zhu
Introducing nanoscale reinforcements into the interface between carbon fiber (CF) and resin is an effective approach to improve the interfacial adhesion of CF composites. In this paper, a facile one-pot polymerization process provides a rapid and efficient method for preparing polyphosphazene microspheres/CF hybrid reinforcement using hexachlorocyclotriphosphazene (HCCP) and bis(4-hydroxyphenyl) sulfone (BPS) as monomers. By the in situ polymerization modification, HCCP and BPS were successfully cross-linked and deposited on the CF surface. Scanning electron microscope and atomic force microscopy images show that poly(cyclotriphosphazene- co -4,4′-sulfonyldiphonel) microspheres were introduced onto the CF surfaces and the surface roughness of fibers is enhanced obviously. Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy confirm that the polymerization between HCCP and BPS has been successfully carried out. The surface modification can significantly increase the fiber roughness, polarity, wettability and surface energy, thus improving the interfacial shear strength of CF/epoxy composites. Meanwhile, the single fiber tensile strength of CF also shows an increase after modification.
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Journal of Materials Chemistry B
·
2024
·
IF 6.1
Qi Wang,Huiyu Yan,Ying Guo,Bei Tian,Jianxi Xiao
Soft tissue deficiencies profoundly impact the daily lives, and mental well-being of patients. Microspheres facilitate collagen synthesis by establishing a conducive environment for fibroblast growth. Existing synthetic polymer microspheres are typically prepared through emulsification and cross-linking at normal temperatures. However, residues of cross-linking agents can adversely affect biocompatibility, thereby limiting their biomedical applications. Agarose has garnered significant attention owing to its biodegradability and excellent biocompatibility. We have for the first time developed high-temperature emulsification coupled with low-temperature gelation for fabrication of agarose microsphere implants with well-controlled size for skin tissue enhancement. The agarose microspheres exhibited favorable sphericity and dispersion, possessing a uniform particle size with an average diameter of 37.24 μm. Furthermore, the microspheres demonstrated commendable injectability and biodegradability. Additionally, the implants displayed remarkable biocompatibility, effectively promoting the proliferation of human foreskin fibroblast-1 (HFF-1) cells. The microspheres exhibited no systemic toxicity and induced no hemolytic or thermogenic reactions. In photoaged mice skin models, the agarose microspheres augmented dermal density, and enhanced skin elasticity. The microspheres showed the capacity to stimulate the regeneration of collagen fibers. The agarose microspheres offer a novel avenue for soft tissue filling and hold significance in the field of tissue engineering and skin regeneration.
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