SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Yiyue Tang,Xiaomei Mo,Lili Yuan,Xueer Huang,Yongkang Yan,Wenying Jin,Lang Zhang,Xinqing Jiang,Jinfang Nie,Yun Zhang
The classical colorimetric assays with Au nanoparticles (AuNPs) are not suitable for analytical systems where the analyte could not induce a red-to-blue color change related to their surface plasmon resonance (SPR). To respond this challenge, herein this study utilizes Tyndall effect (TE) of AuNPs as amplified colorimetric signaling to design a universal visual assay for sensitive detection of trypsin (model analyte). The AuNPs are modified with biotinylated peptides. In the absence of trypsin, streptavidin binds biotin on these probes to form a network-like complex consisting of repeated units of “AuNP-biotin-streptavidin-biotin-AuNP” resulting in a mixture that shows an intense TE response as well a SPR-related red color. Upon trypsin incubation, however, the analyte can cleave the peptide chain to inhibit the streptavidin-mediated production of network-like complex; the resultant red reaction solution containing free AuNP probes would display a weak TE signal. The reduction degree in the TE positively relies on the trypsin level in sample. The results demonstrate that this new equipment-free method only requires a laser pointer pen (for the TE creation) and a smartphone (for portable TE imaging readout) to linearly detect trypsin in a concentration range of 3.9–1000 nM, with an estimated limit of ∼0.63 nM (3σ). The satisfactory recovery of trypsin in actual human serum samples is between 94.93–102.21 %. Such TE-enhanced strategy may significantly expand theoretical scope of widely used AuNP-based visual assays and their applications in various resources-limited settings like point-of-care testing of trypsin for medical diagnosis.
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高影响因子
Acta Pharmaceutica Sinica B
·
2025
·
IF 14.6
Huisong Hao,Yunfei Yi,Yanan Fu,Shengjie Sun,Zhangwen Peng,Jia Tang,Yixuan Fang,Shihao Zhuang,Yaqi Ouyang,Tianqi Wang,Meiying Wu
Abnormal tumor vasculature greatly accelerates tumor progression and diminishes antitumor treatments. Restoring perivascular NO gradients is available to maintain tumor vessel homeostasis and promote tumor vascular normalization. However, exogenously delivering NO strategies lacks the durability to maintain precise NO localization around tumor vessels. Herein, we design a lipid nano delivery system (MC@L) and exploit endothelial transcytosis to deliver metformin (Met) and CaO 2 into tumor vascular endothelial cells (ECs) and tumor cells for achieving tumor vascular normalization-boosted antitumor immunotherapies. The Ca 2+ and Met released in ECs could restore perivascular localization of NO by activating endothelial NOS (eNOS). Additionally, MC@L internalized by tumor cells could cause CaO 2 -induced immunogenic cell death (ICD), together with hypoxia relief and acid neutralization mediated by O 2 generation and H + consumption during CaO 2 degradation, thus further improving the immune effector cell functions under the accompaniment of Met-mediated inhibition of tryptophane uptake in tumor cells. Such a lipid nano delivery system greatly increases the susceptibility of 4T1 tumor-bearing mice to PD-L1 blockade efficacy.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Junli Nie,Gangqiang Zhu,Weibin Zhang,Jianzhi Gao,Peng Zhong,Xiaotao Xie,Yu Huang,Mirabbos Hojamberdiev
Flower-like n -type CeO 2-δ is hybridized with p -type β-Bi 2 O 3 nanoparticles to design a novel β-Bi 2 O 3 /CeO 2-δ p - n heterojunction photocatalyst via a thermal treatment process of Bi/Ce(CO 3 )(OH) precursor. The synthesized β-Bi 2 O 3 /CeO 2-δ exhibits a remarkable enhancement in NO removal and significant suppression of toxic intermediates nitrogen dioxide (NO 2 ) under visible light irradiation. The superior photocatalytic activity of β-Bi 2 O 3 /CeO 2-δ for NO removal is mainly derived from the synergistic effects of the oxygen vacancies (OVs) and p-n heterojunction. The OVs not only enhance the visible light utilization and boost the separation of electron-hole pairs but also improve the adsorption and activation of NO and O 2 . Particularly, the p-n heterojunction is beneficial for the interfacial migration of photogenerated charge carriers. Electron spin resonance (ESR) and trapping experiment reveal that the synergistic effects of OVs and p-n heterojunction can induce the generation of more active species. The electrochemical measurements and density-functional theory (DFT) calculations in-depth confirm that both p-n heterojunction and OVs can accelerate the interfacial charge transfer, resulting in the deep oxidation of reactants. Therefore, the flower-like β-Bi 2 O 3 /CeO 2-δ p-n heterojunction photocatalyst exhibits an advanced photocatalytic activity for deep NO removal and a suppressed NO 2 generation. In addition, monitoring the reaction products by in situ Fourier transform infrared spectrum (FTIR) further validates that the main products of nitrates are formed during the photocatalytic process. This work demonstrates a straightforward approach for construction of p-n heterojunction photocatalysts, which can enhance the effective photocatalytic activity and stability for NO removal.
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高影响因子
CEREAL CHEMISTRY
·
2024
·Vol. 101
·期 5
·1106-1117
·
IF 2.2
Yi Zhou,Xin Wu,Jinxin Shen,Daijun Yang,Qun Lu,Changjiang Long,Rui Liu
Background Black rice is rich in various nutrients, such as anthocyanins, which may undergo some changes during cooking. However, previous studies have been mainly focused on a single substance and paid less attention to the changes in overall nutrient components. Here, we performed widely targeted metabolomics analysis on the chemical compositional changes in a new black rice variety (‘Huamoxiang No.3’) under three cooking modes, including bottom heating (BH), induction heating (IH), and IH + pressure. Results Among the detected 1458 metabolites, 276 and 108 metabolites were differential metabolites after cooking and in three cooking modes, respectively. Cooking increased the contents of most peptides, phenolic acids, and organic acid compounds but significantly decreased those of lipids and flavonoids, particularly anthocyanins. However, phenolic acids such as chlorogenic acid, erucic acid, and p -coumaric acid increased significantly, possibly due to the release of bound phenols and degradation of flavonoids to phenolic acids, such as thermal degradation of C3G to protocatechuic acid and among the three heating modes, the IH + pressure mode resulted in the most significant changes. Conclusion Although cooking reduced the content of flavonoids and anthocyanins, the increase in phenolic acids and other substances contributed to the healthy effect of cooked black rice, and the content of these active substances increased more significantly in the IH + pressure mode.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yuze Luo,Haowen Zhang,Ying Wang,Cheng Jin,Huimin Wang,Zefeng Wang,Yinbang Zheng,Huixu Zhou,Junqin Li,Chunlei Ou,Dinggeng He,Luo Hai
The treatment of diabetic wound infections remains a significant challenge. Nitric oxide (NO) generated by macrophage in body is essential for immune defense and wound healing, making it a promising therapeutic agent for wound infections. However, high glucose level in diabetic wound impairs macrophage function. Here, we construct a macrophage-mimetic living hydrogel microspheres capable of producing NO through a glucose-fueled cascade reaction for efficiently eliminating bacterial infection and accelerating the diabetic wound healing. In our design, glucose oxidase (GOx) and horseradish peroxidase (HRP) are covalently immobilized into the polyacrylamide (PAM) hydrogel microsphere containing poly-L-arginine (P-Arg), followed by encapsulating with M1 macrophage membrane (M) to yield PGHA@M with bacteria-targeting ability. In the presence of glucose, PGHA@M possesses remarkable capability to produce H₂O₂, which is subsequently catalyzed by HRP into hydroxyl radicals ( OH). Meanwhile, the embedded P-Arg is oxidized by OH into NO. PGHA@M demonstrates satisfactory antibacterial and antibiofilm properties. Further in vivo experiments verify that PGHA@M can efficiently eliminate the biofilm bacteria and promote the diabetic wound healing. This study provides a potential strategy to simulate M1 macrophage functions via dual enzymatic cascade reactions, and offers a new perspective for the treatment of diabetic wound infections based on living hydrogel microspheres.
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PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Shuang Luo,Xingyan Liu,Xiangjun Wei,Min Fu,Peng Lu,Xuan Li,Yiming Jia,Qiao Ren,Youzhou He
The novel bifunctional NH 2 -MIL-125/Co(dmgH) 2 composite catalysts with several different Co(dmgH) 2 contents that can simultaneously achieve photocatalytic NO removal and hydrogen production were first prepared by a simple and convenient method. The corresponding physical and chemical properties of the composite catalysts were characterized by SEM, XRD, ESR, in situ DRIFTS, etc. The characterization results indicated that the noble-metal-free Co(dmgH) 2 , which was much cheaper and more available than most noble-metals such as Pt, could be an effective co-catalyst to accelerate the separation of photogenerated electron-hole pairs, further eventually enhancing the photocatalytic efficiency. Under visible-light irradiation for half an hour, the NO removal ratio of NH 2 -MIL-125/Co(dmgH) 2 (3 wt%) increased by 22.7 % compared with the pristine NH 2 -MIL-125 without Co(dmgH) 2 loading. In addition, it was found that Eosin Y dye-sensitized NH 2 -MIL-125/Co(dmgH) 2 (3 wt%) was capable of promoting a hydrogen generation rate of 2195 μmol g −1 h -1 under visible light, which was 12.6 times greater than the original NH 2 -MIL-125. This strategy was expected as an available way to fabricate noble-metal-free molecular complexes with metal-organic frameworks (MOFs) to enhance the photocatalytic NO removal and hydrogen production performance simultaneously.
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Hanzi Liu,Changfu You,Haiming Wang
Transient in-situ IR spectroscopy was performed to analyze the molecular behavior of selective catalytic reduction of NO with NH 3 on V 2 O 5 -WO 3 /TiO 2 catalyst. During NH 3 adsorption, an unexpected growth in the intensity of Lewis acid sites was observed due to the hydrogen migration, which was verified by the DFT calculation and the existence of intermediate species. The comparison between the isolated atmosphere experiments and the simultaneous exposure of NH 3 -NO-O 2 on catalytic monolayer demonstrated that: (i) there exists a pathway for Lewis to Brønsted acid sites transformation at T > 300 °C, (ii) the rate determining step of the SCR cycle involves in the re-oxidation of acid sites by O 2 at 200–300 °C. Generally, the Lewis acids are crucial at high temperatures, while Brønsted acids dominate the overall reaction at lower temperatures. Based on the experimental data and the DFT calculation, a mechanism for ammonia adsorption, acidity conversion and re-oxidation for the redox loop of the de-NOx reaction was proposed. The findings shed light on the understanding of relative strength of NO reduction and acid re-oxidation at different temperatures.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Huirong Zhang,Lin Luo,Zixiong Zhao,Yi Shen,Jinglei Cui,Xiaokai Shi,Xiaoliang Han
The production of high-performance activated carbon (AC) is recognized as an essential strategy for the effective utilization of lignin resources. In this work, the N-doped AC was successfully prepared by blending sodium lignosulfonate (SL) and different ratios of FeCl 3 through one-pot strategy. The AC structure evolution and performance were obtained by a series of characterization and tests. The results showed that, during the activation process, FeCl 3 first complexed with urea and catalyzed the depolymerization of SL, and the rapid pore development was mainly caused by the template, including formed FeCl 2 , Na 2 Fe 3 Cl 8 and Na 6 FeCl 8 . The ratio of FeCl 3 /SL adjusts the AC physicochemical texture. AC-3 processed a super total specific surface area and pore volume, respectively up to 2048 m 2 /g and 1.47 cm 3 /g. Meanwhile, AC-2 showed a higher nitrogen content of 11.4 %. The as-prepared AC shows super desulfurization, denitrification and Cr (Ⅵ) removal ability. AC-2 displayed maximum sulfur capacity and NO conversion, up to 199.1 mg/g and 42.4 %. This enhanced performance is primarily attributed to the pore structure induced by FeCl 3 activation, which provides a foundation for adsorption and catalytic processes, further augmented by the increased active sites due to nitrogen doping. AC-3 achieved an extremely high Cr (VI) adsorption capacity, up to 386.2 mg/g, which was primarily attributed to the redox effect of N-containing and O-containing groups on Cr (VI), in addition, the effect of physical adsorption including pore structure is not neglectable. This study highlights the significant potential of environmentally friendly lignin-based porous carbon materials for removing contaminants in both gas and liquid phases.
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高影响因子
Toxics
·
2024
·Vol. 12
·期 7
·524
·
IF 3.9
Yuanzhen Li,Yinming Fan,Yanhong Wang,Yinian Zhu,Zongqiang Zhu,Shengpeng Mo,Xiaobin Zhou,Yanping Zhang
VOCs can be used instead of ammonia as a reducing agent to remove NO, achieving the effect of removing VOCs and NO simultaneously. Due to the high energy consumption and low photocatalytic efficiency required for conventional thermocatalytic purification, photothermal coupled catalytic purification can integrate the advantages of photocatalysis and thermocatalysis in order to achieve the effect of pollutants being treated efficiently with a low energy consumption. In this study, samples loaded with Co and Mn catalysts were prepared using the hydrothermal method on Fe-MOF with various morphologies. The catalytic performance of each catalyst was analyzed by studying the effects of their physicochemical properties through various characterizations, including XRD, SEM, BET, XPS, H2-TPR, TEM and O2-TPD. The characterization results demonstrated that the specific surface area, pore volume, high valence Co and Mn atoms, surface adsorbed oxygen and the abundance of oxygen lattice defects in the catalysts were the most critical factors affecting the performance of the catalysts. Based on the results of the performance tests, the catalysts prepared with an octahedral-shaped Fe-MOF loaded with Co and Mn showed a better performance than those loaded with Co and Mn on a rod-shaped Fe-MOF. The conversions of acetone and NO reached 50% and 64%, respectively, at 240 °C. The results showed that the catalysts were capable of removing acetone and NO at the same time. Compared with the pure Fe-MOF without Co and Mn, the loaded catalysts showed a significantly higher ability to remove acetone and NO simultaneously under the combination of various factors. The key reaction steps for the catalytic conversion of acetone and NO on the catalyst surface were investigated according to the Mars–van Krevelen (MvK) mechanism, and a possible mechanism was proposed. This study presents a new idea for the simultaneous removal of acetone and NOxby photothermal coupling.
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高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2017
·Vol. 24
·期 8
·7499-7510
·
IF 2.741
Yu Chenglong,Chen Feng,Dong Lifu,Liu Xiaoqing,Huang Bichun,Wang Xinnan,Zhong Shengbang
Manganese-rich MnSAPO-34 molecular sieves were prepared by one-pot synthesis method for NO
x
abatement using the ammonia-selective catalytic reduction (NH 3 -SCR) technology and characterized using ICP, BET, XRD, FE-SEM, H 2 -TPR, NH 3 -TPD, XPS, and DR UV-Vis analyses. The experimental results indicate that the Mn content and chemical state, as well as the surface acidity, of the MnSAPO-34 molecular sieves significantly enhance their DeNO
x
efficiency at low temperatures (ca. 200–300 °C). The manganese-rich MnSAPO-34 was synthesized using a combination of triethylamine and diisopropylamine as the structural directing agents and high Mn loading ( n (MnO)/ n (P 2 O 5 ) = 0.4). The resulting catalyst exhibits the highest activity among all of the samples with a NO
x
conversion value of nearly 95% and a N 2 selectivity that is higher than 90% at 220–400 °C. In addition, this catalyst presents higher NO
x
conversion than the conventional V 2 O 5 -WO 3 /TiO 2 catalysts and other SAPO-based catalysts below 300 °C. Furthermore, the analytical results indicate that the manganese species in the catalyst are mainly in the form of a framework Mn(IV), which could play a significant role in the NH 3 -SCR process as the specific active species. The results suggest that controlling the types and content of the organic amine templates and variations in the surface acidity of the catalysts may significantly enhance the SCR activity at lower temperatures.
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高影响因子
3 Biotech
·
2026
·Vol. 16
·期 8
·328
·
IF 3.1
Zhu Hengzhou,Zhu Xiaodan,Huang Xiaona,Qian Yiyang,Li Jiahui,Zhang Jiaying,Niu Dong,Jin Chunhui
Gastric cancer (GC) is a prevalent malignancy worldwide with high morbidity and mortality. Its insidious onset and lack of sensitive early biomarkers impede routine screening, so most patients present advanced or metastatic disease at first diagnosis, leading to poor prognosis and scarce curative options. Surgery, chemotherapy and targeted therapy serve as standard GC treatments, however their efficacy is restricted by metastasis, adverse reactions and drug resistance, which drives the rapid development of immune checkpoint inhibitor (ICI)-based immunotherapy. Although monoclonal antibodies targeting programmed cell death protein 1/programmed cell death 1 ligand 1 (PD-1/PD-L1) resulted in significant survival benefits for GC patients, some patients exhibited an ineffective response. Our previous study has shown that Weitiao No. 3 (WD-3) enhanced the efficacy of anti-PD-1 immunotherapy by regulating intestinal flora in gastric cancer (GC) mice, in this study, huPBMC-NOG-dKO mice and MGC803 cells were used for in vivo and in vitro functional validation to explore the regulatory mechanism of WD-3 in GC immunotherapy. 16 S rRNA sequencing analysis of mouse fecal samples demonstrated that WD-3 significantly enriched the intestinal abundance of Lachnospiraceae and Ruminococcaceae in GC model mice. Targeted serum metabolomics further verified that WD-3 markedly elevated serum isobutyric acid level in GC mice. Combined with HE staining and tumor growth monitoring, both the intestinal flora mixture consisting of Lachnospiraceae and Ruminococcaceae and isobutyric acid prominently enhanced the anti-tumor efficacy of PD-L1 inhibitors in GC mice. Mechanistically, RNA sequencing and qRT-PCR assays identified ACOX2 as the most significantly up-regulated gene in isobutyric acid-treated MGC803 cells. Functional experiments using CCK-8 assay and flow cytometry revealed that isobutyric acid inhibited the viability and induced apoptosis of MGC803 cells, and such phenotypic changes were effectively rescued by ACOX2 knockdown. Furthermore, results from the CytoTox 96 cytotoxicity assay and ELISA revealed that combined treatment with isobutyric acid and PD-L1 inhibitor elevated the levels of LDH, IL-2, TNF-α, IFN-γ, Perforin 1 and GzmB, while suppressing IL-8, PD-1 and TIM-3 expression in CD8⁺ T cells co-cultured with MGC803 cells. Notably, all these regulatory effects were abrogated when MGC803 cells were transfected with si-ACOX2. These results suggested that WD-3 modulated intestinal flora to elevate isobutyric acid level, thereby enhancing the inhibitory effect of PD-L1 inhibitor on tumor growth and boosting the anti-tumor activity of CD8⁺ T cells by up-regulating ACOX2 expression in GC. These findings may provide novel targets for improving anti-PD-L1 therapy against GC.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Shuang Luo,Chenghua Zhang,Xingyan Liu,Yue Li,Lei Tang,Min Fu,Song Wang,Jie Wu,Mengmeng Xu,Xiaoping Wang,Youzhou He
A novel protonated NH 2 -MIL-125 was fabricated to introduce the moiety with charge and ample hydrogen (–NH 3 + Cl - group) into NH 2 -MIL-125 using HCl vapor obtained by natural volatilization of hydrochloric acid as a protonating reagent, which could exhibit excellent photocatalytic activity in both hydrogen production and NO purification under visible light irradiation. The hydrogen production and NO purification rate of the best protonated NH 2 -MIL-125 were 6762.80 μmol g −1 and 49.17%, respectively, which were 20.53 and 1.84 times as that of the original NH 2 -MIL-125. A series of characterizations, such as Mott-Schottky, PL, FT-IR and in situ DRIFTS , etc. were used to characterize the photoelectrochemical properties of the optimal catalyst, which demonstrated the protonation effect can not only enhance the electron migration rate, but also effectively accelerate photo-generated electron-hole pairs separation in the photocatalytic reaction process. This research believed that using HCl vapor as a facile protonation reagent to introduce the moiety with charge and ample hydrogen (–NH 3 + Cl - ) was a very effective method to decorate MOFs for hydrogen evolution and NO removal.
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高影响因子
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