ANALYTICAL BIOCHEMISTRY
·
2024
·
IF 2.6
Lin Tian,Yang Li,Huiqing Wang,Xinyi Li,Qian Gao,Yaru Liu,Yao Liu,Qing Wang,Cuiping Ma,Chao Shi
Vibrio parahaemolyticus ( V. parahaemolyticus ) is a major foodborne pathogen , which can cause serious foodborne illnesses like diarrhoea. Rapid on-site detection of foodborne pathogens is an ideal way to respond to foodborne illnesses. Herein, we provide an electrochemical sensor for rapid on-site detection. This sensor utilized a pH-sensitive metal-oxide material for the concurrent isothermal amplification and label-free detection of nucleic acids . Based on a pH-sensitive hydrated iridium oxide oxyhydroxide film (HIROF), the electrode transforms the hydrogen ion compound generated during nucleic acid amplification into potential, so as to achieve a real-time detection. The results can be transmitted to a smartphone via Bluetooth. Moreover, HIROF was applied in nucleic acid device detection, with a super-Nernst sensitivity of 77.6 mV/pH in the pH range of 6.0–8.5, and the sensitivity showed the best results so far. Detection of V. parahaemolyticus by this novel method showed a detection limit of 1.0 × 10 3 CFU/mL, while the time consumption was only 30 min, outperforming real-time fluorescence loop-mediated isothermal amplification (LAMP). Therefore, the characteristics of compact, portable, and fast make the sensor more widely used in on-site detection.
研究领域:
电化学
·
生物技术
·
食品安全
·
病原体检测
·
传感器
✓ 已验证
DOI
PubMed
高影响因子
Nanoscale Horizons
·
2017
·Vol. 2
·期 4
·225-232
·
IF 0
Yuanwei Wang,Yihua Zhu,Jianfei Huang,Jin Cai,Jingrun Zhu,Xiaoling Yang,Jianhua Shen,Chunzhong Li
CsPbBr3 perovskite quantum dots (CPBQDs) have exhibited excellent optical properties, which implies their potential as an appealing candidate for fluorescence resonance energy transfer (FRET) based detection. In this work, in order to enhance the subsurface concentration of CPBQDs, which is important for the efficiency of FRET detection, a nanoscale polymethyl methacrylate (PMMA) fiber membrane (d ≈ 400 nm) encapsulated with CPBQDs (CPBQDs/PMMA FM) is fabricated using an electrospinning method. The CPBQD/PMMA FM possesses comparable optical properties to CPBQDs, high quantum yields (88%) and a narrow half-peak width (∼14 nm). The sensing of trypsin is realized via the cleavage of peptide CF6 (Cys–Pro–Arg–Gly–R6G) and an extremely low detection limit of 0.1 μg mL−1 has been reached. Besides, owing to the high efficiency FRET process between the CPBQD/PMMA FM and cyclam–Cu2+, an unprecedented detection limit of Cu2+ has been pushed to 10−15 M. Furthermore, the pH value can be confirmed by the membrane in 10 ppb hydrazide R6G ethanol solution. The excellent optical characteristics of CPBQDs, high CPBQD subsurface concentration of the CPBQD/PMMA FM and robust durability of the PMMA coating all contribute to the outstanding sensitivity and stable detection performance of the CPBQD/PMMA FM.
研究领域:
材料科学
·
化学
·
纳米技术
·
传感器
·
生物医学工程
✓ 已验证
DOI
PubMed
DRUG DELIVERY
·
2020
·
IF 4.902
Yue Cao,Chao Wu,Ying Liu,Lili Hu,Wenjing Shang,Zhanshan Gao,Nan Xia
In this paper, we prepared doxorubicin-loaded folic acid-functionalized pH-sensitive photothermal therapy (PTT) traceable hollow mesoporous silica nanoparticles (DOX-HPCF) as a drug carrier for liver cancer treatment. According to TEM characterization, hollow mesoporous silica nanoparticles (HMSN) are monodispersed spherical particles with hollow structure. In vitro drug release experiments showed that HPCF exhibited pH-sensitive release. Cell uptake experiments showed that HPCF was successfully absorbed by SMMC-7721 cells. In addition, DOX-HPCF significantly inhibited the proliferation of SMMC-7721 cells, and the near-infrared (NIR) light group showed a more obvious inhibitory effect. In vivo anti-tumor experiments showed that DOX-HPCF-assisted PTT inhibited tumor growth significantly. Therefore, HPCF is a promising photothermotherapy carrier for the treatment of liver cancer.
研究领域:
药剂学
·
肿瘤学
·
纳米医学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Tissue Engineering
·
2024
·
IF 6.7
Yue Zhao,Yinlian Yao,Shilong Fan,Xin Shen,Xingxing Chai,Zimin Li,Jiachun Zeng,Jiang Pi,Zhikun Zhou,Gonghua Huang,Hua Jin
The incidence of ulcerative colitis (UC) is rapidly rising worldwide. Oral drug delivery system is a promising approach for treating UC, but it often fails to accumulate to the inflammatory lesions, thus, it is impressive to develop a colon-targeted oral delivery system for preventing systemic toxicity and maintaining UC therapeutics. Here, a negative-charged PLGA nanoparticle system was designed to encapsulate celastrol (Cel), and then chitosan and mannose were coated on the surface of the nanoparticles (MC@Cel-NPs) to endow these nanoparticles with the mucosal adsorption and macrophage targeting abilities. MC@Cel-NPs demonstrate excellent resist decomposition ability against the strong acidic gastrointestinal environment, and accumulates in the specific inflammatory sites through the affinity of electrostatic reaction. After releasing the payload, MC@Cel-NPs could remarkably alleviate the colon inflammation, which was evidenced by the decrease in pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 in both blood and colon sections, and scavenging reactive oxygen species (ROS) in colon cells, including macrophage, neutrophil, T cell, and B cell. This nanoparticle system provided a new approach for treating UC through a Chinese herbal ingredient-related oral delivery manner.
研究领域:
药学
·
纳米技术
·
炎症性肠病
·
药物递送系统
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生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
Polymer Chemistry
·
2012
·Vol. 4
·期 5
·1430-1438
·
IF 5.321
Longlong Chang,Jinjian Liu,Jianhua Zhang,Liandong Deng,Anjie Dong
A novel pH-sensitive, amphiphilic and biodegradable copolymer brush, methoxy poly(ethylene glycol)-block-(polycaprolactone-graft-poly(methacrylic acid)) (mPEG-b-(PCL-g-PMAA)), was developed for NPs for the oral delivery of hydrophobic drugs. The copolymer brush was synthesized by combining ring-opening polymerization (ROP) and atom transfer radical polymerization (ATRP), followed by selective hydrolysis. The structure and composition of the copolymer and its precursors were characterized by 1H-NMR, FT-IR and GPC. The critical micelle concentrations (CMC) of mPEG-b-(PCL-g-PMAA) in aqueous medium were determined to be 6.8 × 10−4 and 9.6 × 10−4 mg mL−1. The copolymer could self-assemble into NPs in aqueous solution with an average size of 104–129 nm, determined by DLS. The morphology of the NPs was spherical, as observed by TEM. The zeta potentials of the NPs were about −25 mV, measured by zeta potential measurements. Ibuprofen (IBU), a poorly water-soluble drug, was chosen as the model drug and encapsulated into the core of the NPs via a nano-precipitation method. The drug loading content (DLC) of the NPs prepared from mPEG-b-(PCL-g-PMAA) reached about 13%, with a drug loading efficiency (DLE) of above 75%. The in vitro release behavior of IBU from the NPs was pH-dependent. Typically, at pH 3.0 (0.01 M), the cumulative release percentage of IBU was about 40% over 12 h, whereas at pH 7.4 (0.01 M), more than 95% was released within 12 h for NPs prepared from mPEG113-b-(PCL91-g-PMAA155). The MTT assay indicated that blank NPs prepared from mPEG-b-(PCL-g-PMAA) did not show significant toxicity against NCL-H460 cells. These results indicated that this new type of pH-dependent polymeric NPs prepared from mPEG-b-(PCL-g-PMAA) has great potential to be used as a drug carrier for the oral administration of hydrophobic drugs.
研究领域:
药剂学
·
高分子科学
·
纳米医学
·
药物递送系统
·
生物材料
·
药代动力学
·
分子药理学
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry A
·
2021
·Vol. 9
·期 9
·5454-5467
·
IF 12.732
Ya'nan Liu,Ruixiang Qu,Xiangyu Li,Huajun Zhai,Shuaiheng Zhao,Yen Wei,Lin Feng
Superwetting materials that possess the property of flow-through catalysis have been applied widely for the treatment of water contamination, due to their dual functions of simultaneous separation of oil/water phases and degradation of organic pollutants. However, there is an unavoidable antinomy between the separation flow rate and degradation efficiency, which is called the dilemma of “trade-off”. Concretely, a high flow rate is essential for gravity driven separation whereas thorough degradation generally demands the contact time between materials and targets to be long enough. Herein, we proposed a vanadium oxide based dual-mesh system which is composed of one catalysis mesh folding onto one switch mesh. The switch mesh is pH controllable that turns “on” in a basic environment and turns “off” under acid or neutral conditions. Initially, the switch mesh was “off” and thus polluted water could be blocked above the switch mesh and degraded by the catalysis mesh completely. Once detecting the end of degradation, the switch was turned on by pH regulation and as a result water flowed through the system with a high flow rate and simultaneously formed a barrier to the oil phase, demonstrating the superb ability of the system to solve the “trade-off”. The response of the whole system is expeditious and reiterative, and meanwhile the degradation and separation capabilities are outstanding and durable. We believe that this flexible integration of catalytic capability and pH-responsive wettability can provide inspiration for extremely efficient elimination of various pollutants from water in practice.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
BIOMATERIALS
·
2017
·
IF 8.402
Ruirui Zhao,Tao Li,Guirong Zheng,Kai Jiang,Lulu Fan,Jingwei Shao
Co-delivery multiple drugs using nanocarriers has been recognized as a promising strategy for cancer treatment to enhance therapeutic efficacy. In this study, a pH sensitive mesoporous silica nanoparticles (MSN) based controlled release nanoparticles for co-delivery of sorafenib (SO), a multi-tyrosine kinase inhibitor, and ursolic acid (UA), a sensitive agent for SO, was developed, which was decorated with pH sensitive chitosan (CS) and lactobionic acid (LA) targeting to asialoglycoprotein receptor (ASGPR) over-expressing hepatocellar carcinoma cells (denoted as USMNs-CL). The nanocomplex enhanced bioavailability of hydrophobic drugs, efficient tumor cell targeting and exhibited pH-responsive function and sustained release profile. USMNs-CL showed synergistic cytotoxicity and could attenuate the adhesion, migration of ASGPR over-expressing liver cancer SMMC-7721 cells at non-toxic concentrations. Moreover, the complex nanoparticles significantly increased the cellular apoptosis and down-regulated the expression of EGFR and VEGFR2 proteins related with cell proliferation and tumor angiogenesis . In vivo , compared with UA or SO alone, the nanocomplex significantly reduced the tumor burden in hepatocellular carcinoma (HCC) H22 tumor-bearing mice model and inhibited the lung metastasis in the H22 lung metastasis models. Overall, co-delivery of UA and SO by MSN-CS-LA nanocarriers could provide a promising strategy for HCC combinational therapy, especially for the HCC metastasis chemoprevention.
研究领域:
肿瘤学
·
药剂学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Jiawei Liu,Guo Du,Tiehong Chen
Ordered mesoporous silica is widely used in catalysis, adsorption, and biomedicine, among which SBA-15 (Santa Barbara Amorphous-15) is one of the most widely studied. However, the synthesis of SBA-15 often requires strong acid (hydrochloric acid or sulfuric acid), which will not only corrode industrial equipment but also pollute the environment with the wastewater containing strong acid and halogen (sulfur). Here, we demonstrate a green synthetic strategy for SBA-15 under weakly acidic conditions through an anionic assembly route. With the assistance of poly(acrylic acid) (PAA) and 3-aminopropyltrimethoxysilane (APMS), the pH value of the synthesis system can be increased to 4–5, which is a mild near-neutral condition. In addition, halogen-free synthesis using organic acids is also achieved. The powder X-ray diffraction (XRD), transmission electron microscopy (TEM), and N2 sorption characterizations show that the obtained SBA-15 has good texture properties, with a specific surface area of 430–500 m2/g and ordered 6–8 nm mesopores, which is similar to SBA-15 synthesized in traditional strong acid. This strategy provides a facile and environmentally friendly route for the large-scale production of ordered mesoporous materials.
研究领域:
材料科学
·
化学
·
环境科学
✓ 已验证
DOI
高影响因子
BIOMATERIALS
·
2019
·
IF 10.273
Jie An,Yong-Guo Hu,Cheng Li,Xiao-Lin Hou,Kai Cheng,Bin Zhang,Ruo-Yun Zhang,Dong-Yu Li,Shao-Jun Liu,Bo Liu,Dan Zhu,Yuan-Di Zhao
Sonodynamic therapy (SDT) has rapidly developed as a powerful alternative to traditional photodynamic therapy due to its intrinsically deeper tissue-penetration. However, single SDT dose is incapable of radical cure because the long-term hypoxia of tumor limits its therapeutic effect. Herein, we developed a biomimetic nanoplatform with dual pH/ultrasound response, homologous targeting and low phototoxicity for combined nitric oxide (NO) gas therapy with SDT to solve the problem. This nanoplatform is composed of zeolite imidazole framework-8 material embedded with nitrosoglutathione (GSNO) and chlorin e6 (Ce6) by one-step encapsulation, and then wrapped by homologous tumor cell membrane . In vitro and in vivo experiments indicate that the biomimetic nanoplatform has excellent biocompatibility and shows higher retention in tumor by homologous targeting. Importantly, it can sustainably release the encapsulated drug in acidic tumor microenvironment and accelerate degradation by ultrasound (US). Furthermore, NO released from GSNO and reactive oxygen species generated by Ce6, which are both triggered by US, react with each other to produce highly reactive peroxynitrite to inhibit the growth of tumor. Moreover, by repeated US irradiation, the tumor hypoxia can be relieved for a much-longer term, resulting in an effective gas-sonodynamic combined treatment. This study fully utilizes the advantages of US, providing a new strategy for high-performance cancer therapy.
研究领域:
癌症治疗
·
纳米医学
·
生物医学工程
·
药剂学
✓ 已验证
DOI
PubMed
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 47
·4931-4937
·
IF 3.532
Rongrong Xing,Tingyu Xue,Peng Ye,Li Yang,Runqin Wang,Xuan Chen,Shuang Hu
Chlorogenic acid and caffeic acid often coexist in traditional Chinese medicines (TCMs) and play roles as antioxidation, antiviral, antitumor and anti-inflammatory agents. Due to their low content and the presence of structural analogues, they cannot be effectively separated by conventional extraction methods. Molecularly imprinted polymers, as synthesized receptors with antibody-like binding properties, have significant advantages in separating structural analogues. However, the harsh imprinting conditions easily induced the degradation of chlorogenic acid. Therefore, caffeic acid was used as an epitope template to replace chlorogenic acid for imprinting. Boronic acid-functionalized magnetic nanoparticles (MNPs) were selected as substrates, which could not only facilitate the immobilization and removal of the templates by pH regulation, but also achieve rapid separation under an external magnetic field. Tetraethyl orthosilicate was selected as an imprinting monomer which allowed for precise control of the thickness of the imprinting layer by adjusting the imprinting time. The prepared epitope-imprinted MNPs showed excellent specificity, in combination with high performance liquid chromatography, have been successfully applied to the selective separation and detection of chlorogenic acid and caffeic acid in TCMs.
研究领域:
分析化学
·
纳米技术
·
药学
·
中医药
✓ 已验证
DOI
高影响因子
PROGRESS IN ORGANIC COATINGS
·
2021
·
IF 5.161
Chunling Li,Xiyu Zhao,Cheng Meng,Tengfang Zhang,Shuangqing Sun,Songqing Hu
The stimuli-responsive release could reduce the waste of the corrosion inhibitor and realize the accurate repair of corroded region. In this work, hollow mesoporous organosilica nanoparticles (HMON) were first synthesized by silica-etching chemistry tailoring method. Then, HMON were encapsulated with 2-mercaptobenzothiazole (MBT) corrosion inhibitor molecules to build an intelligent corrosion inhibitor system, i.e., MBT@HMON. The microstructure and frame composition of the synthesized HMON were characterized. After the successful encapsulation of MBT into HMON, the release kinetics of MBT from HMON under different dithiothreitol (DTT) concentration and pH conditions were investigated. Finally, the corrosion inhibition performance of MBT@HMON for copper alloy was evaluated in 3.5 wt% NaCl solution by weight loss test and electrochemical impedance spectroscopy (EIS). The results show that the controlled release of corrosion inhibitor MBT from HMON could be realized by tuning the redox and pH conditions of corrosion medium. When the appropriate concentration of MBT@HMON was added into the corrosion medium (1.6 g·L −1 in this work), the corrosion of cooper alloy was greatly inhibited. This simple and general strategy of introducing corrosion inhibitors loaded HMON directly into the corrosion medium offers a new prospect for the application of nanocontainers in the intelligent corrosion protection of metallic materials.
研究领域:
材料科学
·
腐蚀科学
·
纳米技术
·
化学工程
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Yunsu Ma,Huanghuang Xu,Bo Sun,Silin Du,Shuai Cui,Li Zhang,Ning Ding,Dongzhi Yang
The combination of photodynamic therapy (PDT) and chemodynamic therapy (CDT) has been continuously explored in the antibacterial aspect and has achieved more effective antibacterial effect than a single therapy. We design a pH-responsive O2 and H2O2 self-supplying zeolitic imidazolate framework-67 (ZIF-67) nanosystem for PDT/CDT of wound infection. Under the acidic inflammatory conditions, ZIF-67 can degrade to produce Co2+ and release CaO2 and graphene quantum dots (GQDs). The exposed CaO2 reacted with water to generate H2O2 and O2. The self-supplied O2 alleviates hypoxia at the site of inflammation and enhances external light-initiated GQD-mediated PDT, while H2O2 was catalyzed by endogenous Co2+ to produce hydroxyl radicals for Co2+-triggered CDT. In vitro and in vivo experiments confirm that CaO2/GQDs@ZIF-67 has a combined PDT/CDT effect. The antibacterial mechanism indicates that bacteria post-treated with CaO2/GQDs@ZIF-67 may be sterilized by reactive oxygen species-mediated oxidative stress and the leakage of bacterial contents. The experiments also find that CaO2/GQDs@ZIF-67 may activate the immune response and enhance the therapeutic effect by activating the cyclic GMP–AMP synthase–stimulator of interferon genes signaling pathway.
研究领域:
纳米技术
·
生物医学工程
·
药学
·
传染病
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）