iScience
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2024
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IF 4.6
Ruobing Wang,Dan Su,Yueze Liu,Hua Huang,Jiangdong Qiu,Zhe Cao,Gang Yang,Hao Chen,Wenhao Luo,Jinxin Tao,Guihu Weng,Taiping Zhang
Summary Nuclear factor kappa B (NF-κB) plays a pivotal role in the development of pancreatic cancer, and its phosphorylation has previously been linked to the regulation of NUAK2. However, the regulatory connection between NF-κB and NUAK2, as well as NUAK2’s role in pancreatic cancer, remains unclear. In this study, we observed that inhibiting NUAK2 impeded the proliferation, migration, and invasion of pancreatic cancer cells while triggering apoptosis. NUAK2 overexpression partially resisted apoptosis and reversed the inhibitory effects of the NF-κB inhibitor. NF-κB transcriptionally regulated NUAK2 transcription by binding to the promoter region of NUAK2. Mechanistically, NUAK2 knockdown remarkably reduced the expression levels of p -SMAD2/3 and SMAD2/3, resulting in decreased nuclear translocation of SMAD4. In SMAD4-negative cells, NUAK2 knockdown impacted FAK signaling by downregulating SMAD2/3. Moreover, NUAK2 knockdown heightened the sensitivity of pancreatic cancer cells to gemcitabine, suggesting that NUAK2 inhibitors could be a promising strategy for pancreatic cancer treatment.
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NEW JOURNAL OF CHEMISTRY
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2025
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IF 2.5
Xinjia Zhang,Jingmin Ma,Jian Hao,Tenghao Ma,Jing Wang,Shimiao Peng
In this study, La–Fe2(MoO4)3@CoMoO4 composite core–shell structured electrode materials were successfully prepared by the hydrothermal method. The research results show that the introduction of lanthanum not only enhances the conductivity and charge transfer rate of Fe2(MoO4)3@CoMoO4, but also improves the electrolyte permeability and ion transport capacity by introducing oxygen vacancies and lattice defects. In addition, the core–shell structure of nanorods combined with nanosheets increases the specific surface area of the material, enhances the structural stability of the material, provides more transmission channels for ions, and improves the electrochemical reaction rate and cycle stability of the material. In the three-electrode test system, the specific capacitance of La–Fe2(MoO4)3@CoMoO4 nanomaterials only slightly decreased from 1985 F g−1 to 1963 F g−1 after 10 000 charge–discharge cycles at a current density of 5 A g−1, with a capacitance retention rate of 98.8%, demonstrating excellent cycle life. Moreover, the composite material of molybdenum dioxide (MoO2) and carbon nanotubes (CNTs) combines the excellent conductivity and ion diffusion performance of both, providing strong support for efficient energy storage. The asymmetric supercapacitor composed of La–Fe2(MoO4)3@CoMoO4 and CNTs/MnO2 anode exhibits excellent comprehensive performance, with an energy density as high as 50.2 Wh kg−1 and a power density reaching 12 000 W kg−1, providing an effective strategy for the design and development of high-performance supercapacitors.
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Nature Communications
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2025
·Vol. 16
·期 1
·1-13
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IF 15.7
Jiang Yilin,Xi Ruonan,Yin Jinlin,Sun Chen,Li Yukong,Wu Chao,Zhang Chi,Fei Honghan
Three-dimensional lead halide hybrids exhibit excellent photophysical properties but suffer from inherent instability. In contrast, two-dimensional layered lead halides offer enhanced environmental stability, yet their strongly bound excitons restrict efficient charge transport. Here we present a covalent intercalation strategy involving the benchmark photosensitizer [Ru(bpy) 3 ] 2+ into a layered lead halide framework, featuring cationic [Pb 23 X 42 ] 4+ (X − = Cl − or Br − ) layers pillared by [Ru(bpy) 3 ] 2+ ligands via Pb 2+ -carboxylate coordination. This hybrid material achieves nearly full visible-light absorption and efficient photoinduced charge transfer from [Ru(bpy) 3 ] 2+ to the lead halide layers. This affords efficient CO 2 -to-CO photoreduction with an apparent quantum efficiency of ~3.0% at 500 nm, exceeding the performance of all previously reported organolead halide photocatalysts. Mechanistic studies indicate that the [Ru(bpy) 3 ] 2+ ligands enhance charge transport to Pb 2+ sites, facilitating CO 2 activation and reducing the reaction barrier for the *COOH intermediate. This work establishes a paradigm for intercalation chemistry in robust layered lead halide hybrids.
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APPLIED SURFACE SCIENCE
·
2025
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IF 6.9
Qiangli Lv,Haoran Guo,Duo Xu,Yuling Zhai,Hua Wang,Tao Zhu,Xing Zhu,Kongzhai Li,Zhihong Yang,Zhishan Li
The urea oxidation reaction (UOR) is considered as an alternative to the oxygen evolution reaction for high-efficiency hydrogen production, because it can simultaneously achieve wastewater treatment and hydrogen production. However, the slow kinetics of the UOR hinder its widespread adoption due to relatively complex molecule, it is urgent to rational design and preparation of high-performance UOR catalysts. Herein, we report a simple synthesis of the NiMn-LDH@Rh(OH) 3 heterostructure and a systematic investigation of urea-assisted electrolytic hydrogen production， significantly enhances the efficiency of urea electrolysis. The amorphous Rh(OH) 3 structure provides a higher density of active sites and greater catalytic surface exposure, while the synergistic interaction between Rh(OH) 3 and NiMn-LDH improves both electrical conductivity and catalytic performance. The catalyst of NiMn-LDH@Rh(OH) 3 –3 requires only 1.29 V overpotential to achieve a current density of 10 mA cm −2 in a 1.0 M KOH and 0.33 M urea solution, surpassing other catalysts. Furthermore, it demonstrates remarkable stability with minimal potential increase during 48-hour chronopotentiometric tests at 100 mA cm −2 . This work making the developed strategy promising for the rational design of highly active electrocatalysts for green hydrogen production and the treatment of urea-rich wastewater.
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JOURNAL OF ENDODONTICS
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2022
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IF 4.422
Qianli Zhang,Chongyang Yuan,Li Liu,Shipeng Wen,Xiaoyan Wang
Introduction In this study, we generated a 3-dimensional (3D) collagen fibrous scaffold for potential pulp regeneration and investigated the influence of various pore sizes of these scaffolds on proliferation, odontoblastic differentiation of human dental pulp cells (hDPCs), and subsequent tissue formation during pulp regeneration. Methods Electrospinning followed by freeze-drying was used to fabricate 3D fibrous collagen scaffolds. hDPCs were cultured on these scaffolds. Cell growth was detected by a Cell Counting Kit-8 assay and observed via scanning electron microscopy. Odontogenic genes and protein expression were analyzed by real-time reverse transcription polymerase chain reaction and immunofluorescence staining. The formation of mineralized nodules was tested by von Kossa staining, scanning electron microscopy, and energy-dispersive X-ray microanalysis. Subcutaneous transplantation of the seeded scaffold/tooth fragments into nude mice was performed to observe tissue formation for pulp regeneration. Results Collagen 3D fibrous scaffolds with 3 distinct mean pore sizes (approximately 20 μm, 65 μm, and 145 μm) were fabricated, which showed good biocompatibility and bioactivity. Scaffolds with larger mean pore sizes of 65 and 145 μm improved hDPC ingrowth and proliferation, with the 65-μm scaffold group presenting the highest level of odontogenic gene expression ( DSPP and DMP-1 ), protein expression (DMP-1), mineralized area ratio, and vascular pulplike tissue formation after 6 weeks of subcutaneous implantation. Conclusions The pore size of collagen 3D fibrous scaffolds significantly affected cell adhesion , proliferation, odontoblastic differentiation, and tissue rehabilitation. Scaffolds with a mean pore size of 65 μm presented superior results and could be an alternative for pulp regeneration.
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Frontiers in Chemistry
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2020
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IF 3.693
Wang Yang,Ravikumar Yuvaraj,Zhang Guoyan,Yun Junhua,Zhang Yufei,Parvez Amreesh,Qi Xianghui,Sun Wenjing
D-allulose, which is one of the important rare sugars, has gained significant attention in the food and pharmaceutical industries as a potential alternative to sucrose and fructose. Enzymes belonging to the D-tagatose 3-epimerase (DTEase) family can reversibly catalyze the epimerization of D-fructose at the C3 position and convert it into D-allulose by a good number of naturally occurring microorganisms. However, microbial synthesis of D-allulose is still at its immature stage in the industrial arena, mostly due to the preference of slightly acidic conditions for Izumoring reactions. Discovery of novel DTEase that works at acidic conditions is highly preferred for industrial applications. In this study, a novel DTEase, DTE-CM, capable of catalyzing D-fructose into D-allulose was applications. In this study, a novel DTEase, DTE-CM, capable of catalyzing D-fructose into D-allulose was DTE-CM on D-fructose was found to be remarkably influenced and modulated by the type of metal ions (co-factors). The DTE-CM on D-fructose was found to be remarkably influenced and modulated by the type of metal ions (co-factors). The 50°C from 0.5 to 3.5 h at a concentration of 0.1 mM. The enzyme exhibited its maximum catalytic activity on D-fructose at pH 6.0 and 50°C from 0.5 to 3.5 h at a concentration of 0.1 mM. The enzyme exhibited its maximum catalytic activity on -fructose at pH 6.0 and 50°C with a Kcat/Km value of 45 mM−1min−1. The 500 g/L D-fructose, which corresponded to 30% conversion rate. With these interesting catalytic properties, this enzyme could be a promising candidate for industrial biocatalytic applications.
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JOURNAL OF NANOBIOTECHNOLOGY
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2025
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IF 12.6
Deng Yu,Jiang Xinyu,Che Zhenzhen,Shang Yaqi,Hu Mengting,Wang Wentian,Yu Weixian,Yang Bai,Liu Xinchan
Periodontitis is a chronic inflammatory disease strongly linked to the sustained accumulation of reactive oxygen species (ROS). Eliminating excessive ROS and modulating the periodontal microenvironment to suppress inflammation represents a promising therapeutic approach for the treatment of periodontitis. Fisetin (FIS) is a naturally occurring flavonoid known for its strong antioxidant and anti-inflammatory effects. However, its poor water solubility and low bioavailability limit its therapeutic efficacy. Carbon dots (CDs), as an emerging nanomaterial, offer advantages such as simple synthesis, good biocompatibility, and low cost. To overcome the limitations of FIS, this study synthesized fisetin-derived carbon dots (FIS-CDs) via a hydrothermal method. The resulting FIS-CDs exhibits excellent water solubility, favorable biocompatibility, and demonstrates excellent ROS-scavenging capability without the need for further modification. In vitro FIS-CDs significantly reduced intracellular ROS levels, alleviate oxidative stress, maintain mitochondrial homeostasis, and suppress the generation of inflammatory cytokines. Furthermore, FIS-CDs exhibit excellent osteogenic potential. In vivo experiments confirmed that FIS-CDs markedly alleviated periodontal inflammation and oxidative damage, suppressed alveolar bone loss, and promoted regeneration of periodontal tissues. Mechanistically, FIS-CDs facilitate the activation of mitophagy by downregulating the SUMOylation of sirtuin 3 (SIRT3). This modulation contributes to the restoration of mitochondrial function, which in turn mitigates cellular injury and limits the secretion of inflammatory mediators. Collectively, these findings underscore the therapeutic promise of FIS-CDs as a safe and effective nanomaterial for periodontitis treatment and suggest a novel molecular target for future drug development. Graphical abstract
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Probiotics and Antimicrobial Proteins
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2025
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IF 4.4
Zhang Haipeng,Zhao Xiaoou,Wang Xiaoyu,Wang Xinyue,Gu Jinyang,Liu Zhongliang,Kong Lingcong,Chen Jingrui,Ma Hongxia
The widespread use of commercial antibiotics has led to the emergence of multidrug resistance in pathogenic bacteria, posing a significant threat to human health and underscoring the urgent need for alternative antimicrobial agents. In this study, a bacteriocin-producing strain, Bacillus velezensis FS-3 ( B. velezensis FS-3), was isolated from soil in Changbaishan, China. Fermentation conditions were optimized to enhance both bacteriocin activity and yield. A novel bacteriocin, PFS-3, was purified from B. velezensis FS-3 using hydrochloric acid precipitation, organic solvent extraction, and preparative reversed-phase high-performance liquid chromatography. Liquid chromatography-mass spectrometry/mass spectrometry analysis determined its molecular weight to be 929.16 Da, and amino acid sequencing of this peptide revealed eight amino acids (STYLFEGL). To date, the biological properties of PFS-3 have not been reported. We demonstrate here that PFS-3 exhibits low toxicity and remarkable stability under diverse conditions, including variations in temperature, pH, and the presence of metal ions or organic reagents. PFS-3 displayed broad-spectrum antimicrobial activity, with particularly strong activity against Gram-negative bacteria. Notably, its minimum inhibitory concentration against multidrug-resistant Escherichia coli B2 (MDR E. coli B2) was 16 μg/mL. Mechanistic investigations revealed that bacteriocin PFS-3 exerts bactericidal effects on MDR E. coli B2 by disrupting cardiolipin in the outer membrane of the cells. Furthermore, in vivo experiments demonstrated that PFS-3 significantly improved survival rates in infection models of MDR E. coli . In conclusion, PFS-3 is a newly identified bacteriocin with strong antibacterial activity, high stability, safety, and a favorable therapeutic index, highlighting its potential applications in the food industry and biopharmaceuticals for combating MDR E. coli infections.
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JOURNAL OF BIOSCIENCES
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2020
·Vol. 45
·期 1
·1-7
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IF 1.645
Jin Jia-Yuan,Chen Ye,Wang Xing-You,Li Chen-Ming,Chen Wei-Lin,Li Li
Herein, we found that serum concentration of superoxide dismutase 3 (SOD3) was significantly reduced in children with mycoplasma pneumonia (MP) infection. To study the roles of SOD3 in inflammatory regulation of MP infection, human A549 type II alveolar epithelial cells were stimulated with 10 7 CCU/ml of MP to build MP infection in vitro . Secretion of pro-inflammatory cytokine interleukin (IL)-8 and tumor necrosis factor (TNF)-α were measured via enzyme-linked immunosorbent assay (ELISA) to assess the inflammatory response of A549 cells. Levofloxacin (LVFX) was used as an anti-inflammatory drug while recombinant TNF-α was used as an inflammatory promotor in MP-infected cells. Transcriptional activity of nuclear factor (NF)-кB was assessed by detecting protein levels of nuclear NF-кB and cytoplasm NF-кB using Western blot analysis. Our data suggested that the expression of SOD3 mRNA and protein, as well as content of SOD3 in cultured supernatant, were time-dependently inhibited in MP-infected A549 cells. However, lentiviruses-mediated SOD3 overexpression alleviated inflammatory response of MP-infected A549 cells, and prevented the unclear translocation of NF-кB, as evidenced by obviously reducing the production of IL-8 and TNF-α in cell cultured supernatant, as well as decreasing nuclear NF-кB while increasing cytoplasm NF-кB.
Inspiringly, SOD3 overexpression induced anti-inflammatory effect and the inactivation of NF-кB was similar to that of 2 μg/ml of LVFX, but reversed by additional TNF-α treatment. Therefore, we can conclude that transcriptional activity of NF-κB was the underlying mechanism, by which SOD3 regulated inflammatory response in MP infection in vitro .
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Microbiome
·
2023
·Vol. 11
·期 1
·1-23
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IF 15.5
Hu Jun,Chen Jianwei,Xu Xiaojian,Hou Qiliang,Ren Jing,Yan Xianghua
Background The intestinal epithelial barrier confers protection against the intestinal invasion by pathogens and exposure to food antigens and toxins. Growing studies have linked the gut microbiota to the intestinal epithelial barrier function. The mining of the gut microbes that facilitate the function of intestinal epithelial barrier is urgently needed.Results Here, we studied a landscape of the gut microbiome of seven pig breeds using metagenomics and 16S rDNA gene amplicon sequencing. The results indicated an obvious difference in the gut microbiome between Congjiang miniature (CM) pigs (a native Chinese breed) and commercial Duroc × [Landrace × Yorkshire] (DLY) pigs. CM finishing pigs had stronger intestinal epithelial barrier function than the DLY finishing pigs. Fecal microbiota transplantation from CM and DLY finishing pigs to germ-free (GF) mice transferred the intestinal epithelial barrier characteristics. By comparing the gut microbiome of the recipient GF mice, we identified and validated Bacteroides fragilis as a microbial species that contributes to the intestinal epithelial barrier. B. fragilis-derived 3-phenylpropionic acid metabolite had an important function on the enhancement of intestinal epithelial barrier. Furthermore, 3-phenylpropionic acid facilitated the intestinal epithelial barrier by activating aryl hydrocarbon receptor (AhR) signaling.Conclusion sThese findings suggest that manipulation of B. fragilis and 3-phenylpropionic acid is a promising strategy for improving intestinal epithelial barrier.
Video Abstract
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Cell Chemical Biology
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2020
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IF 7.739
Giorgia Croppi,Yueyang Zhou,Rong Yang,Yunfei Bian,Mingtao Zhao,Youtian Hu,Benfang Helen Ruan,Jing Yu,Fang Wu
Summary H 2 S-producing enzymes in bacteria have been shown to be closely engaged in the process of microbial survival and antibiotic resistance. However, no inhibitors have been discovered for these enzymes, e.g., 3-mercaptopyruvate sulfurtransferase (MST). In the present study, we identified several classes of inhibitors for Escherichia coli MST (eMST) through high-throughput screening of ∼26,000 compounds. The thiazolidinedione-type inhibitors were found to selectively bind to Arg178 and Ser239 residues of eMST but hardly affected human MST. Moreover, the pioglitazone of this class concentration dependently accumulates the 3-mercaptopyruvate substrate and suppresses the H 2 S and reactive sulfane sulfur products in bacteria. Importantly, pioglitazone could potentiate the level of reactive oxygen species in cellulo and consequently enhance the antimicrobial effects of gentamicin and macrophages in culture. This study has identified the bioactive inhibitor of eMST, paving the way for the pharmacological targeting of eMST to synergistically control the survival of E. coli .
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
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IF 8.1
Bingqin Li,Liangxing Jiang,Nan Xiao,Siliang Liu,Zongliang Zhang,Fangyang Liu,Michael L. Free
Traditional technologies of lithium extraction from salt lakes suffer the low efficiency of Li + /Mg 2+ separation and high energy consumption. We introduce a novel electrodialysis process that leverages Li 1.3 Al 0.3 Ti 1.7 (PO 4 ) 3 (LATP, one of lithium superionic conductors) and incorporates an aided charge balance (ACB) system, all under low voltage and ultra-low current conditions. The remarkable ion selectivity of LATP, coupled with the enhanced recovery ratio facilitated by ACB, collectively empower this technology to achieve extremely high separation efficiency and remarkably low energy consumption. In a simulated pristine brine, the average Li/Mg separation coefficient reached 5924, accompanied by an exceptionally low energy consumption of only 0.80 kWh·kg −1 Li . Furthermore, this technique enabled the production of battery-grade Li 2 CO 3 with an outstanding purity of 99.93 %, achieved through a streamlined process comprising only two stages.
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