JOURNAL OF BIOMATERIALS APPLICATIONS
·
2018
·
IF 2.082
Xiaolong Liu,Yiran Xia,Lulu Liu,Dongmei Zhang,Zhaosheng Hou
The purpose of this study is to offer a novel kind of polyurethane with improved surface blood compatibility for long-term implant biomaterials. In this work, the aliphatic poly(ester-urethane) (PEU) with uniform-size hard segments was prepared and the PEU surface was grafted with hydrophilic poly(ethylene glycol) (PEG). The PEU was obtained by chain-extension of poly(ɛ-caprolactone) (PCL) with isocyanate-terminated urethane triblock. Free amino groups were introduced onto the surface of PEU film via aminolysis with hexamethylenediamine, and then the NH2-grafted PEU surfaces (PEU-NH2) were reacted with isocyanate-terminated monomethoxyl PEG (MPEG-NCO) to obtain the PEG-grafted PEU surfaces (PEU-PEG). Analysis by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, and gel permeation chromatography were performed to confirm the chemical structures of the chain extender, PCL, PEU, and PEU-PEG. Additionally, the influence of aminolysis on the physical-mechanical properties of PEU films was investigated. Two glass transition temperatures and a broad endothermic peak were observed in the differential scanning calorimetry curves of PEU, which demonstrated a microphase-separated and semicrystalline structure, respectively. The PEU-PEG film exhibited excellent mechanical properties with an ultimate stress of ∼39 MPa and an elongation at break of ∼1190%, which was slightly lower than that of PEU, indicating that the aminolysis has little influence on the tensile properties. Evaluation of the blood compatibility of the films by bovine serum albumin adsorption and the platelet adhesion test revealed that the PEG-grafted surface had improved resistance to protein adsorption and excellent resistance to platelet adhesion. In vitro degradation tests showed that the PEU-PEG film could maintain its mechanical properties for more than six months and only lost ∼25% weight after 18 months. Due to the excellent mechanical properties, good blood compatibility and slow degradability, this novel kind of polyurethane hold significant promise for long-term implant biomaterials, especially soft tissue augmentation and regeneration.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Aiyang Liu,Xiuhang Chai,Shuang Zhu,Ping-tan Chin,Mengxue He,Yong-Jiang Xu,Yuanfa Liu
Astaxanthin (AST) has outstanding antioxidant and anti-inflammation bioactivities, but the low biocompatibility and stability limit its application in foods. In this study, N-succinyl-chitosan (NSC)-coated AST polyethylene glycol (PEG)-liposomes were constructed to enhance the biocompatibility, stability, and intestinal-targeted migration of AST. The AST NSC/PEG-liposomes were uniform in size, had larger particles, greater encapsulation efficiency, and better storage, pH, and temperature stability than the AST PEG-liposomes. AST NSC/PEG-liposomes exerted stronger antibacterial and antioxidant activities against Escherichia coli and Staphylococcus aureus than AST PEG-liposomes. The NSC coating not only protects AST PEG-liposomes from gastric acid but also prolongs the retention and sustained release of AST NSC/PEG-liposomes depending on the intestinal pH. Moreover, caco-2 cellular uptake studies showed that AST NSC/PEG-liposomes had higher cellular uptake efficiency than AST PEG-liposomes. And AST NSC/PEG-liposomes were taken up by caco-2 cells through clathrin mediated endocytic, macrophage pathways and paracellular transport pathway. These results further proved that AST NSC/PEG-liposomes delayed the release and promoted the intestinal absorption of AST. Hence, AST PEG-liposomes coated with NSC could potentially be used as an efficient delivery system for therapeutic AST.
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EUROPEAN POLYMER JOURNAL
·
2019
·
IF 3.621
Heling Li,Jinchi Dai,Qi Xu,Cuifen Lu,Guichun Yang,Feiyi Wang,Junqi Nie,Xingjian Hu,Nianguo Dong,Jiawei Shi
A series of high-strength and hydrolytic degradable POSS-PEG hybrid hydrogels were successfully fabricated by Michael addition reaction of 4-arm-PEG-maleimide with thiol-terminated PEG-functionalized POSS cross-linkers in aqueous phase. The cross-linkers, which are hydrophilic, multifunctional and contain esters, were prepared by linking hydrophilic PEG with different chain length onto hydrophobic POSS and terminating with a thiol group. All the prepared hybrid hydrogels were achieved with a porous structure and high mechanical strength, and the G′ of POSS-PEG 500 hybrid hydrogel reached a maximum of 15,000 Pa, which is much higher than our previous POSS-PEG hybrid hydrogels prepared by monofunctional POSS. The swelling ratio and hydrolytic degradation rate of hybrid hydrogels increased with the increase of PEG chain length. In vitro cell culture showed that all the hybrid hydrogels had good biocompatibility. Thus, these hybrid hydrogels exhibited the potential for tissue engineering scaffolds.
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International Journal of Nanomedicine
·
2018
·
IF 4.37
Fei Tong,Suhuan Liu,Bing Yan,Xuejun Li,Shiwei Ruan,Shuyu Yang
Introduction Insulin has shown antioxidation and cytoprotective effects to decrease heart ischemia/reperfusion injury (HI/RI) in diabetes mellitus (DM), but the role of insulin/poly(ethylene glycol)-carboxymethyl chitosan (PEG-CMCS) on HI/RI in DM is not known. This research explored whether insulin/PEG-CMCS revealed a protective effect on HI/RI in DM through ornithine decarboxylase (ODC)/polyamine systems.Materials and methods Diabetes was induced via streptozotocin (STZ) in Sprague Dawley (SD) rats, which suffered from HI via blocking the left circumflex artery for 45 minutes, followed by 2 hours of reperfusion. α-Difluoromethylornithine-ethylglyoxal bis (guanylhydrazone) (DFMO-EGBG) and insulin/PEG-CMCS were administered to diabetic rats to explore their roles on severity of HI/RI.Results Insulin could be fleetly and efficiently loaded via the nanocarrier PEG-CMCS at pH =6, showing efficient loading and stable release. In addition, insulin/PEG-CMCS showed significant hypoglycemic activity in diabetic rats. On the other hand, ischemia/reperfusion obviously augmented the contents of creatine kinase (CK), lactic dehydrogenase (LDH), putrescine (Pu), myocardial infarct size, and NF-κB and spermidine/spermine N′-acetyltransferase (SSAT) expressions and decreased the levels of spermine (Sp), polyamine pools (PAs), heart rate (HR), coronary blood flow (CF), left ventricular developed pressure (LVDP), and ODC expression, compared with Sham. Administration of insulin and insulin/PEG-CMCS both reduced the contents of CK, LDH, Pu, myocardial infarct size, and NF-κB and SSAT expressions and increased the levels of Sp, PAs, HR, CF, LVDP, and ODC expression, while insulin/PEG-CMCS significantly indicated the protective results, and DFMO-EGBG showed the opposite effects.Conclusion The research showed that insulin/PEG-CMCS could play a protective effect on HR/RI in diabetic rats via its antioxidative, antiapoptotic, and anti-inflammatory roles and modulating ODC/polyamine systems.
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PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
·
2017
·Vol. 35
·期 3
·1700337
·
IF 4.474
Zhuo Li,Zhenglin Li,Lei Sun,Baosheng Du,Yuanlin Wang,Gongyuan Zhao,Dengfeng Yu,Sisi Yang,Ye Sun,Miao Yu
Thermo-chemotherapy combining photothermal therapy (PTT) with chemotherapy has become a potent approach for antitumor treatment. In this study, a multifunctional drug-delivery nanoplatform based on polyethylene glycol (PEG)-modified mesoporous silica-coated bismuth selenide nanoparticles (referred to as Bi 2 Se 3 @mSiO 2 -PEG NPs) is developed for synergistic PTT and chemotherapy with infrared thermal (IRT) imaging of cancer cells. The product shows no/low cytotoxicity, strong near-infrared (NIR) optical absorption, high photothermal conversion capacity, and stability. Utilizing the prominent photothermal effect, high-contrast IRT imaging and efficient photothermal killing effect on cancer cells are achieved upon NIR laser irradiation. Moreover, the successful mesoporous silica coating of the Bi 2 Se 3 @mSiO 2 -PEG NPs cannot only largely improve the stability but also endow the NPs high drug loading capacity. As a proof-of-concept model, doxorubicin (DOX) is successfully loaded into the NPs with rather high loading capacity (≈50.0%) via the nanoprecipitation method. It is found that the DOX-loaded NPs exhibit a bimodal on-demand pH- and NIR-responsive drug release property, and can realize effective intracellular drug delivery for chemotherapy. The synergistic thermo-chemotherapy results in a significantly higher antitumor efficacy than either PTT or chemotherapy alone. The work reveals the great potential of such core–shell NPs as a multifunctional drug-delivery nanosystem for thermo-chemotherapy.
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JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
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2017
·Vol. 17
·期 4
·2613-2619
·
IF 1.483
Feng Zhao,Qi Jianqi,Huang Zhangyi,Xie Xiumin,Wei Nian,Lu Tiecheng
Single-phase nano-crystalline yttria-stabilized zirconia (YSZ) powder was prepared by co-precipitation method using zirconium oxychloride (ZrOCl2 · 8H2O) and yttrium nitrate (Y(NO3)3 · 6H2O) as raw materials and ammonium
bicarbonate (NH4HCO3) and liquor ammonia (NH4OH) as the precipitators. In order to get the powder with favorable dispersibility, polyethylene glycol (PEG, HO(CH2CH2O)
n
H) with different molecular weights and amounts was
used as a dispersant agent in the synthesis process and its effects on the YSZ powder was investigated. The scanning electron micrograph (SEM) images showed that the degree of agglomeration and the mean crystal size of YSZ powder varied with the amount and the molecular weight of PEG. The
appropriate amount and molecular weight of PEG were given by comparing the dispersibility and crystal size distribution of powders. In the process, one also could find that the promotion of dispersion was insensitive to the molecular weight, and all PEG (400–2000) can exert an ability
to reduce the agglomeration. The proper amount of PEG400, PEG600, PEG1000, PEG2000 was 3 wt%, 3 wt%, 2 wt%, 2 wt%, respectively. The transmission electric microscope studies in conjunction with the XRD analyzes verified the low degree of agglomeration and the size of YSZ powder varied between
10~20 nm.
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NPG Asia Materials
·
2017
·Vol. 9
·期 5
·e383
·
IF 9.157
Yin Ting,Zhang Qian,Wu Haigang,Gao Guo,Shapter Joseph G,Shen Yulan,He Qiaozhi,Huang Peng,Qi Wen,Cui Daxiang
Effectively prolonging the residence time of nanoprobes in the tumor region and reducing the accumulation of nanoprobes in the vital organs (for example, lung, liver and spleen) is crucial for high-efficiency photodynamic therapy (PDT) of cancer. Herein, we systematically report an ultra-small and highly stable nanoplatform with diameters of 4, 8 and 13 nm that exhibited excellent photodynamic therapeutic efficacy using Fe 3 O 4 @polymer-NPO/PEG-Glc@Ce6 nanoprobes. Based on the small size effect, the nanoprobes displayed lower cytotoxicity and excellent biocompatibility. Owing to the synergistic virtues of markedly active targeting and intrinsic small size effect, the Fe 3 O 4 @P-NPO/PEG-Glc@Ce6 nanoprobes can effectively prolong their residence time in the tumor region and reduce accumulation in the normal organs. Benefitting from the small size effect, the synthesized Fe 3 O 4 @P-NPO/PEG-Glc@Ce6 nanoprobes exhibited excellent tumor-targeting capability and photodynamic therapeutic efficacy by inhibiting the growth of tumors in mice under visible red light irradiation with a relatively lower power. The successful application of the small size effect in Fe 3 O 4 @P-NPO/PEG-Glc@Ce6 nanoprobes to significantly improve the PDT efficiency in our strategy suggests new building blocks for PDT of tumors and paves a new way for clinical therapies and translation in the near future.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·Vol. 22
·期 1
·1-13
·
IF 10.6
Lian Jingge,Li Meng,Duan Meng,Sun Yaqian,Wang Zilin,Guo Xinyu,Li Jingchao,Gao Guo,Li Kangan
Adoptive cellular immunotherapy as a promising and alternative cancer therapy platform is critical for future clinical applications. Natural killer (NK) cells have attracted attention as an important type of innate immune regulatory cells that can rapidly kill multiple adjacent cancer cells. However, these cells are significantly less effective in treating solid tumors than in treating hematological tumors. Herein, we report the synthesis of a Fe3O4-PEG-CD56/Avastin@Ce6 nanoprobe labeled with NK-92 cells that can be used for adoptive cellular immunotherapy, photodynamic therapy and dual-modality imaging-based in vivo fate tracking. The labeled NK-92 cells specifically target the tumor cells, which increases the amount of cancer cell apoptosis in vitro. Furthermore, the in vivo results indicate that the labeled NK-92 cells can be used for tumor magnetic resonance imaging and fluorescence imaging, adoptive cellular immunotherapy, and photodynamic therapy after tail vein injection. These data show that the developed multifunctional nanostructure is a promising platform for efficient innate immunotherapy, photodynamic treatment and noninvasive therapeutic evaluation of breast cancer.
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JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2015
·
IF 1.648
Zhifei Xie,Huameng Gong,Mingxing Liu,Hongda Zhu,Honghao Sun
In this paper, a novel drug-loaded material (MSNs-SS-PEG) was obtained by grafting the thiol-linked methoxy polyethylene glycol (MeOPEG-SH) onto the thiol-functionalized mesoporous silica nanoparticles (MSNs-SH) via the disulfide bond linker. In our designed experiment, three different chain lengths of PEG (PEG1000, PEG5000, and PEG1000-PEG5000) were used. The silica materials were characterized by Fourier transform infrared spectroscopy (FT-IR), dynamic light scattering, field emission scanning electron microscopy, transmission electron microscopy, nitrogen adsorption–desorption measurements, and X-ray diffraction. The morphology of the MSNs-SS-PEG was spherical with an average diameter of about 150 nm. Due to the covalent modification of hydrophilic MeOPEG, the MSNs-SS-PEG was coated by a thin polymer shell, showing stable and inerratic MCM-41 type mesoporous structure as well as high specific surface areas and large pore volumes. Moreover, the releases of doxorubicin hydrochloride (DOX) from these materials at 10 mM of glutathione were investigated. The PEG functionalization could effectively cap drugs in the mesoporous channels. The release of DOX from the MSNs-SS-PEGn revealed redox-responsive characteristic. The obtained results showed that the MSNs-SS-PEG might be promising drug delivery carrier materials, which could play an important role in the development of drug delivery.
研究领域:
纳米医学
·
药剂学
·
生物材料
·
药物递送
·
分子影像
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肿瘤治疗
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转化医学
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SOLID STATE IONICS
·
2018
·
IF 2.751
Huihui Zeng,Xiaoxiao Ji,Fangchang Tsai,Qunchao Zhang,Tao Jiang,Robert K.Y. Li,Hengchong Shi,Shifang Luan,Dean Shi
The poor contact between the cathode and the solid polymer electrolyte (SPE) is one of the primary causes for the low specific capacities of lithium ion batteries . In this work, a composite cathode material containing LiFePO 4 nanoparticles encapsulated by PEG-based polymer electrolyte (coated LiFePO 4 ) are prepared in order to increase the interfacial contact area between SPE and cathode materials. The LiFePO 4 /SPE/Li half-cell battery with coated LiFePO 4 (C-LFP) and uncoated LiFePO 4 (U-LFP) are assembled and their electrochemical performances are studied. The optimal LiFePO 4 /SPE weight ratio in the composites cathode is 60/15. When the cathode thickness is 100 μm, the specific capacity of battery with C-LFP can reach up to 138 mAhg −1 , which is 2.5 times higher than that with U-LFP under 1C charge-and-discharge rate at 60 °C. Moreover, the capacity retention of C-LFP is 91% after 300 cycles, while that of U-LFP is only 18%. Large cracks emerge in the cathode with U-LFP, while no damage can be found in that with C-LFP after 300 cycles at 1C. This should be ascribed to the existence of polymer buffer layers among LiFePO 4 nanoparticles to alleviate the stress induced by their volume changes during the charge-and-discharge cycling process. This enhancement effect increases along with the increase of cathode thickness. When the cathode thickness increases up to 200 μm, the specific capacity of battery with C-LFP cathode is almost 4 times higher than that with U-LFP.
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2016
·Vol. 134
·期 8
·
IF 1.866
Tian Zhong,Yang Jiao,Lingling Guo,Jiamin Ding,Zhuping Nie,Lianjiang Tan,Ran Huang
To address concern of the dispersion property of hydrophilic drugs of excess dose loaded in a hydrophobic poly(lactic acid) (PLA) matrix, this work developed a PLA and PLA -b -polyethylene glycol (PEG) composite scaffold ( ) and studied its carrier properties for aspirin as a model hydrophilic drug. The porous functional scaffolds were prepared from PLA and PLA -b -PEG solutions with the dose of 5, 10, and 15 wt % aspirin preloaded. The products and control samples of pure PLA with the same loading amount for comparison were characterized by scanning electron microscopy and X-ray diffraction to examine the miscibility and porous structure. Rapid degradations in a strongly basic solution were performed to determine the actual loading amount and the encapsulation ratio. The in vitro release in phosphate buffer saline (PBS) at 37.5 °C indicated that the addition of amphiphilic block polymer may efficiently enhance the dispersion property and stabilize the release of hydrophilic drugs, especially with a high loading dose. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134 , 44489.
研究领域:
生物材料
·
药物递送
·
高分子科学
·
组织工程
·
制药技术
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BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2026
·
IF 2.5
Han Liu,Zhuo Li,Xilin Zhu,Ying Liu,XiaoPan Wu
Background & aims Caspase-10 (CASP10) participates in death receptor–mediated apoptosis, but the relevance of the CASP10 coding variant rs13006529 (T > A; p.Leu522Ile) to PEG-IFN-α response in chronic hepatitis B (CHB) is unknown. We assessed this association and whether differential apoptotic activity explains variant-associated HBV burden. Methods rs13006529 was genotyped in 262 patients receiving PEG-IFN-α with or without nucleos(t)ide analogues. Allele and genotype frequencies were compared by χ 2 tests; sustained virological response (SVR) was analyzed by univariable and multivariable logistic regression. HBV-infected HepG2-NTCP cells expressing empty vector or either CASP10 variant were compared; variant-expressing cells received vehicle or zVAD-fmk. Apoptosis was assessed by flow cytometry, caspase-3/7 activity, and Western blotting. HBV total RNA and pgRNA, intracellular DNA, cccDNA, and HBsAg/HBeAg were measured by RT-qPCR, qPCR, Southern blotting, and ELISA, respectively. Results The A allele was more frequent in patients without than with SVR (30.6% vs 17.1%; P = 0.002) and independently associated with lower odds of SVR (adjusted OR per A allele, 0.50; 95% CI, 0.27–0.86; P = 0.017). SVR rates were 36.9% in TT and 19.8% in AT/AA carriers (P = 0.003). Leu522-expressing cells showed greater apoptosis and lower HBV total RNA, pgRNA, intracellular DNA, and cccDNA than Ile522-expressing cells. zVAD-fmk attenuated the between-variant difference in apoptosis and partially narrowed the corresponding differences in HBV RNA and intracellular DNA. Conclusions rs13006529 was independently associated with PEG-IFN-α response. Differential caspase-dependent apoptotic activity partly accounts for variant-associated HBV burden differences.
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