Food Bioscience
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2025
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IF 5.9
Yangjincan Liu,Yuqing Huang,Haiming Hu,Huabing Yang,Lei Xiong,Junping Zheng,Tianxiang Zhu,Zhigang Zhang,Hongtao Liu
s Chenpi is a traditional medicinal and edible food in China. Chenpi polysaccharide (CP) is one of the main active components of Chenpi. This study established a fermentation process using Bacillus licheniformis to obtain fermented Chenpi polysaccharides (FCP) with a reduced degree of polymerization. FCP is an acidic polysaccharide exhibiting a molecular weight of 3720 Da. The three predominant monosaccharides in FCP were arabinose (31.75%), galactose (24.77%), and galacturonic acid (20.94%). According to comprehensive NMR studies, its main chain primarily comprised two types of residues: →4)-α-GalA p- (1→ and →4)-α-Gal p , while its side chains started with →2)-α-Fuc p- (1→ or →2)-β-Rha p- (1→ residues, which were further linked to extending residues such as →4)-β-Ara p- (1→ or →5)-α-Ara f- (1→, and ultimately terminated with terminal residues T-β-GalA p , T-β-Glc p , or T-α-Ara f . Compared to commercial pectic polysaccharides (PP), FCP exhibited higher solubility, a looser structure, and lower viscosity. In vitro gut microbiota fermentation demonstrated that FCP substantially enhanced the proliferation of beneficial bacteria, including Paraprevotella and Lachnospiraceae , while suppressing pathogenic bacteria like Shigella . Furthermore, FCP enhanced short-chain fatty acids (SCFAs) production by gut microbiota, particularly acetic and propionic acids. In the single-strain fermentation experiment, FCP promoted the growth of Lactococcus lactis , Pediococcus acidilactici , Parabacteroides distasonis , and Bacteroides thetaiotaomicron . Metabolomic analysis of Bacteroides thetaiotaomicron fermentation broth further revealed that FCP altered the composition of bacterial metabolites, enhancing the biosynthesis of beneficial metabolites, including propionate, 4'-O-methylglucoliquiritigenin, and cyclokievitone hydrate. This study indicated that B. licheniformis fermentation improves the physical and prebiotic properties of CP, providing new insights for the bioprocessing of Chenpi.
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FOOD HYDROCOLLOIDS
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2025
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IF 12.4
Jilong Lu,Meizhen Xie,Lipin Chen,Yong-Huan Yun
Colorimetric indicators of real-time food spoilage detection are typically fabricated using chromogenic agents or nanoparticles; however, their potential migration into food raises considerable safety concerns. Although structurally colored functional biomaterials, which do not pose such risks, represent promising alternatives, their application in food spoilage detection remains largely unexplored. In this study, a structurally colored chitosan membrane (CSM) was obtained from shrimp shells, and its responsiveness to acids and aldehydes was investigated. Fresh coconut water was used to evaluate the performance of CSM as a colorimetric spoilage indicator. Results indicate that CSM is a mesoporous material with a Bouligand structure and a deacetylation degree of 83.93 %. The specific surface area, average pore volume, and average pore size of CSM were 0.44 m 2 /g, 0.001 cm 3 /g, and 9.67 nm, respectively. CSM exhibited chromatic responsiveness to acids at a low pH of 3 due to the protonation of –NH 2 groups, as well as to aldehydes via the formation of imine bond (C=N). Acid and aldehyde exposure altered the crystalline polymorphism of chitosan in CSM. After 18 h of storage at room temperature, the membrane successfully detected spoilage in fresh coconut water through visible color changes, which can be attributed to the synergistic effects of organic acids and aldehydes accumulated during spoilage. These findings demonstrate the effectiveness of CSMs in detecting food spoilage. To our knowledge, this study is the first to demonstrate the potential application of structurally colored CSMs for detecting food spoilage, offering a novel approach for developing food spoilage indicators.
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Chinese Medicine
·
2025
·Vol. 20
·期 1
·1-23
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IF 5.7
Xu Jin-Di,Gao Xiao-Qin,Zhong Rong-Ling,Zhou Jing,Wang Ting,Chen Can,Yao Wei-Feng,Geng Ting,Zhang Yi,Li Song-Lin,Zhang Li
Background Shi–Zao–Tang (SZT), a classical formula of Traditional Chinese Medicine orally used for treating malignant ascites effusion (MAE), is made by mixing the powder of Kansui Radix (KR), Euphorbiae Pekinensis Radix (EPR) and fried Genkwa Flos with the decoction of Jujubae Fructus. According to Chinese Pharmacopoeia, vinegar-processed KR and EPR should be used in oral administration. However, toxicity and efficacy of SZT containing vinegar-processed KR and EPR (VSZT) versus SZT in MAE rats, and the potential mechanisms of VSZT against MAE, remain unknown. Here, we comparatively studied the quality, toxicity and efficacy of SZT and VSZT, and explored the potential mechanisms of VSZT against MAE. Methods Main components in SZT and VSZT were quantified by liquid chromatographic coupled with triple quadrupole tandem mass spectrometry. The intestinal toxicity and efficacy of SZT and VSZT were comparatively investigated in MAE rats. Specially, intestinal toxicity was evaluated by intestinal barrier function, histopathology and oxidative damage. The efficacy was investigated by amount of ascites, indices in excretion, intestinal motility and inflammation. The potential mechanisms of VSZT treats MAE were explored through integration of metabolomics, 16S rRNA and Western blotting analysis. Results VSZT contains less 3- O - EZ and more ingenol than SZT. VSZT showed reduced intestinal toxicity than SZT in MAE rats. Both SZT and VSZT indiscriminately decreased the amount of ascites and ascitic inflammatory cytokines, promoted urination and defecation, increased fecal water content and intestinal motility. VSZT reversed endogenous metabolism and gut microbiota disorders, down-regulated colonic cAMP, PKA, p-CREB/CREB and AQP3, as well as mesenteric p-VEGFR2/VEGFR2, p-SRC/SRC, and p-VE-cadherin/ VE-cadherin in MAE rats. Conclusion VSZT preserved the efficacy of SZT on MAE with lower intestinal toxicity. VSZT increased water excretion and decreased MAE formation to alleviate MAE through regulating gut microbiota, restoring tryptophan and tyrosine metabolism disorders, and affecting cAMP-PKA-CREB-AQP3 and VEGFA-VEGFR2-SRC-VE-cadherine pathway.
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JOURNAL OF APPLIED MICROBIOLOGY
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2025
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IF 3.2
Chen Yang,Yang Jian-xia,Yv Tian,Zhang Xin-rui,Gong Gui-zhen,Zhu Yuan-ting,Yao Jin,Wu Yi,Zhang Yv-han
AimsThis study aimed to investigate the capacity of lactic acid bacteria (LAB) isolated from yak calves to metabolize xylo-oligosaccharides (XOS) and to identify potential probiotic strains that could enhance the gut health of yak calves.Methods and ResultsLAB isolates were recovered from fifteen fecal samples collected from yak calves. Their capacity to utilize XOS as the sole carbon source was evaluated, and their metabolic characteristics, molecular mechanisms, and functional properties associated with XOS metabolism were examined through integrated phenotypic and genomic analyses. Consequently, forty-one LAB isolates were identified from ten of the fifteen yak calves and tested for XOS utilization. Among these, 51.22% (21/41) exhibited species- and strain-specific XOS utilization, including Limosilactobacillus mucosae (10 isolates), Lactobacillus brevis (10 isolates), and Lactiplantibacillus plantarum (1 isolates). L. mucosae YT200 showed the highest acid production (pH 4.50) and short-chain fatty acid accumulation, particularly acetic acid (745.00 ± 16.24 mM). Genomic analysis revealed three gene clusters encoding GH43 α-L-arabinofuranosidase, GH51 α-L-arabinofuranosidase, and GH43 β-xylosidase, enabling the hydrolysis of arabinosyl-substituted and linear XOS. Strain YT200 also demonstrated antagonism against common gut pathogens including Escherichia coli and Staphylococcus aureus, sensitivity to seven antibiotics, and no transferable resistance genes.ConclusionThis study reveals the mechanistic basis of XOS metabolism in L. mucosae YT200 and identifies L. mucosae YT200 as a promising candidate for synbiotic development.
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CERAMICS INTERNATIONAL
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2025
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IF 5.6
Yumin An,Mingyao Song,Jiabao Xu,Yongshuai Xie
In this paper, Ce-Zr oxide ceramic fiber aerogels (CZOf) with effective thermal insulation properties were successfully prepared by a simple two-step method of solution blow spinning and pyrolysis. In the spinnable precursor cerium-zirconium carboxylate, the organic carboxylic acid ligands and metal inorganic salts enhanced its structural stability through coordination interactions. The effect of thermal treatment temperature and Ce doping amounts on the crystal structure of CZOf were analyzed based on XRD. At the same time, the microstructures of the CZOf heat treated at different temperatures were analyzed by SEM in detail. In addition, CZOf exhibited a compressive stress of 24.9 kPa under 80 % compressive strain, and still showed a compressive stress of 20.2 kPa after 20 compression cycles. The room-temperature thermal conductivity of CZOf was in the range of 31.4–38.3 mW m −1 K −1 . The CZOf with a thickness of 10 mm could reduce the hot surface temperature from 1200 °C to about 210.5 °C after 10min, demonstrating excellent thermal insulation performance.
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Journal of Future Foods
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2025
·
IF 7.2
Chao Yu,Zongshuo Li,Ying Wang,Yanxia Shu,Zhenghui Liu,Weidong Li
Cyclophosphamide (CTX) is a common anticancer drug that can cause immunosuppression and disrupt the intestinal microbiota when taken long-term or at high doses. Lonicerae Japonicae Flos polysaccharides (LJFP), identified as the principal bioactive constituents of L. Japonicae Flos, have emerged as promising immunomodulatory agents. Herein, we explored the molecular mechanisms through which LJFP alleviate CTX-induced immunosuppression in mice. As determined via gas chromatography (GC), LJFP contain arabinose, rhamnose, ribose, xylose, mannose, fructose, galactose, and glucose. LJFP mitigated the decline in body weight and immune organ indexes in CTX-induced mice, while promoting cytokine production. Moreover, LJFP stimulated immunity by reshaping the gut microbiota of mice, where Muribaculaceae and Alistipes played key roles. LJFP also increased short-chain fatty acid (SCFA) levels in the mouse colon and feces. Network pharmacology analyses identified 31 core targets and relevant signaling pathways implicated in the treatment of immunosuppression. Molecular docking indicated strong binding of the eight monosaccharide components to key targets EGFR, HSP90AA1, and IL2. Notably, fifteen of these targets specific to the gut further suggested that the mechanism through which LJFP ameliorates immunosuppression is intricately linked to the gut microbiota. The current findings highlight the potential of LJFP as a functional food or alternative therapeutic against immunosuppression.
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JOURNAL OF ALLOYS AND COMPOUNDS
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2024
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IF 5.8
Yue Li,Junhao Zhou,Xiaoping He,Youzhou He,Jiajia Jing,Xingyan Liu,Siqi Li,Siping Wei
Under the global dual-carbon goal, photocatalysis technology is being employed as a green technology for NO removal and hydrogen production . However, the application of photocatalysts is often limited by the weak response to visible light and the low separation efficiency of photogenerated carriers. In this paper, we designed a simple and efficient photocatalyst Au@NM-125/PdTCPP for NO removal and hydrogen evolution . By adding PdTCPP molecule with excellent light absorption ability combine with MIL-125-NH 2 (Ti), the introduction of PdTCPP expands the absorption range of visible light. On the other hand, the introduction of Au NPs with surface plasmon resonance effect promotes electron transport between PdTCPP and MIL-125-NH 2 (Ti), thus greatly improving the photocatalytic performance. Compared with the NO removal efficiency of the original NM-125 (22.06 %), the NO removal rate of NM-125/PdTCPP was 63.72 %, while the NO removal rate of Au@NM-125/PdTCPP was the highest, reaching 73.23 %. What’s more, Au@NM-125/PdTCPP also demonstrated excellent hydrogen evolution performance (12.659 mmol/g, in 4 h), with a hydrogen evolution effect 2.14 times that of NM-125/PdTCPP (5.911 mmol/g, in 4 h) and 6.85 times that of the initial material NM-125 (1.848 mmol/g, in 4 h). Based on the results of UV-Vis spectroscopy, Mott-Schottky and XPS spectrum, we proposed a possible photocatalytic mechanism, the suitable band gap value of MIL-125-NH 2 (Ti) and PdTCPP makes them form a Z-scheme heterojunction , where electrons flow from PdTCPP to MIL-125-NH 2 (Ti), and Au NPs acts as an electron bridge to promote more electron transfer, thus improving the photocatalytic performance. It is a practicable strategy to achieve excellent photocatalytic performance by combining metalloporphyrin molecules as semiconductors directly with MOFs to form heterojunctions and adding Au NPs as electronic bridges, which is also valuable for environmental remediation and energy production.
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JOURNAL OF ETHNOPHARMACOLOGY
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2024
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IF 4.8
Jingying Shi,Yawen Liu,Zijia Zhang,Xianchun Zhong,Yuhan Cao,Hui Ni,Qingqing He,Zhaojun Wang,Yameng Liu,Qinhua Chen,Jianming Wei,Haibo Wang,Likun Gong,Cen Xie,Jinjun Hou,Wanying Wu
Ethnopharmacological relevance Zexie-Baizhu Decoction (AA), a Chinese Classical Formula composed of Alisma orientalis (Sam.) Juzep . and Aractylodes Macrocephala Koidz in the specific ratio of 5:2, has a long history of use in treating metabolic disorders. Recent studies have demonstrated AA’s ameliorative effects on non-alcoholic fatty liver disease (NAFLD); however, the mechanism underlying its action on the gut and adipose tissue, key regulators of metabolism, have not been fully explored. Aim of the study This study aimed to investigate the mechanisms by which AA regulates the homeostasis of gut and adipose tissue in NAFLD. Materials and Methods AA (1500 mg/kg/day) or vehicle was administrated to the high-fat diet-induced and normal chow-fed mice (C57BL/6J). Plasma, the liver, gut microbiota, bile acids, and short-chain fatty acids in the gut, were systematically investigated. RNA sequencing analysis, reverse transcription quantitative real-time PCR, and Western Blotting were performed on the epididymal white adipose tissues (eWAT) to explore AA’s influence on NAFLD. Lipidomics of the liver and eWAT were analyzed by liquid chromatography-mass spectrometry and desorption electrospray ionization mass spectrometry imaging. Results Our study demonstrated that AA administration effectively alleviated liver injury induced by NAFLD, as evidenced by reduced hepatic fat accumulation and inflammation. Mechanistically, AA modulated the composition of the gut microbiota, promoting the growth of beneficial bacteria such as Akkermansia muciniphila and restoring the balance between Firmicutes and Bacteroidetes. Furthermore, AA regulated the levels of bile acids and short-chain fatty acids in the intestine, plasma, and liver. Correspondingly in the eWAT, AA administration activated bile acid receptor ( Gpbar1 ) and short-chain fatty acid receptor ( Ffar2 ), facilitating lipid breakdown and attenuating triglyceride accumulation. Transcriptome analysis revealed that AA influenced gene expression related to fatty acid metabolism, thermogenesis, insulin resistance, AMPK signaling, and the tricarboxylic acid (TCA) cycle, thereby improving NAFLD at the transcriptional level. Additionally, AA treatment significantly altered the lipid composition in the liver, reducing levels of diacylglycerols, triacylglycerols, phosphatidylserines, and cholesterol esters, while increasing levels of phosphatidic acids, phosphatidylethanolamines, and sphingomyelins. Conclusion Our study builds a connection between the gut and adipose tissue to understand the mechanism of AA on alleviating NAFLD, providing new insights into the development of targeted therapies for this condition.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-12
·
IF 15.7
Zhang Tianhao,Tian Yuan,Zhang Chong,Yan Tingting,Yan Hanwen,Zhang Guoliang,Li Jie,Li Zengxi,Wang Gang,Li Chunshan,Zhang Suojiang
Electronic properties of active sites profoundly influence catalytic production of value-added chemicals; however, rational description and modulation is still a significant challenge. Herein, we propose an effective metal-ion-chelation strategy guided by density functional theory prediction and in situ Raman observation to structurally tailor and quantitatively correlate the electronic properties of active sites in ionic liquid. Comprehensive characterizations and theoretical calculations, in combination with electronic properties–performance correlations, reveal the electronic peculiarity of nitrogen and oxygen centers can be controllably restructured for remarkable improvement of catalytic performance at near-ambient condition. The turnover frequency is increased by two folds with deactivation rate suppressed by more than one half, while the kilogram-scaled recycling pilot achieves similar performance for the probe methacrolein synthesis. This strategy further exhibits excellent applicability and tolerance in other substrates with representative functional groups. Our work expresses the significance of electronic properties and provides a valid regulation approach for ionic liquid catalysis.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2025
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IF 4.6
Yawen Zhang,Hongli Zhang,Jiangong Ma,Jing Zhao,Huajuan Yin,Xu Jing,Xiaowen Wang
With the advent of new-generation green extraction solvents, deep eutectic solvents (DESs) present significant prospects for the extraction of bioactive substances. In this study, saponins were extracted from oats using an ultrasonic-assisted method with green DESs, replacing conventional toxic organic solvents. Adding sodium chloride markedly reduced solubility, resulting in the separation of two phases from the extract: DES and water. The saponins were separated into the water phase, while the DES was reused in subsequent extractions, supporting extractant recycling and promoting sustainable development. Adding salt offers a more gentle, convenient, and cost-effective alternative to current phase separation methods, such as pH adjustment, temperature variation, and carbon dioxide or nitrogen gas application. Optimizing the extraction and recovery parameters yielded the highest oat saponin extraction efficiency at 18.73 mg g –1 , and the recovery was 86.4 %. When compared with ethanol extracts, DES extracts exhibited superior antioxidant activity. Lastly, the greenness of this extraction method was confirmed through three greenness evaluation programs of greenness. The extraction method based on emerging responsive solvents can be extended to other hydrophilic bioactive substances.
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JOURNAL OF CHROMATOGRAPHY A
·
2025
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IF 4
Huanhuan Jiang,Qibin Li,Yang Yang,Jingcong Dai,Li Wang,Richard D Cannon,Xiaojing Dong,Ting-li Han,Lina Hu
Short-chain fatty acids (SCFAs) are prominent regulators of host physiology and are closely related to the pathophysiology of many metabolic diseases. SCFAs are predominately generated by microbial fermentation of dietary fiber in the gut. Therefore, efficient, reproducible, and affordable analytical methods are needed to identify and quantify SCFAs in a complex fecal sample. In this study, five different SCFAs (acetic acid, butyric acid, propionic acid, valeric acid, and isobutyric acid) were compared using six different extraction methods (HCl-DE, NaOH-Hexane, H 3 PO 4 -Butanol, H 3 PO 4 -EA, NaHCO 3 -MTBE, and SPME) based on gas chromatography-mass spectrometry (GC-MS) analysis. The specificity, linearity, recovery, precision, and sensitivity of different analytical approaches were systematically assessed using SCFA chemical standards and further validated with human fecal samples, ensuring cross-species applicability. Our results revealed that no single method performed optimally for all SCFAs, but rather, distinct methods exhibited compound-specific advantages. Moreover, this study provides a practical guideline for balancing recovery, reproducibility, and extraction efficiency for method selection based on research purposes. The H 3 PO 4 -Butanol method displayed superior recovery accuracy for valeric acid (100.16 ± 0.81% and 101.82 ± 4.83% recovery rate for low and median spiked concentrations, respectively). It also exhibited minimum intra-day repeatability (0.92% to 5.67% RSD values) and good linearity (R 2 =0.94-0.99) across chemical mixtures. However, it showed the best extracted efficiency for butyric acid and best sensitivity for isobutyruc acid in fecal samples. Moreover, the H 3 PO 4 -EA method showed the highest recovery accuracy for isobutyric acid (103.21 ± 11.07% to 104.79 ± 3.59% recovery rate for all three different spiked concentrations) and the smallest inter-day repeatability (3.37% to 7.25% RSD values). The SPME method exhibited superior recovery accuracy for acetic acid (81.16 ± 21.17% to 94.08 ± 10.92% recovery rate for all three different spiked concentrations) and extracted the highest levels of SCFAs for acetic acid, propionic acid, and isobutyric acid. Finally, the application of these methods in human clinical samples revealed significant alterations of SCFA levels in patients with missed abortion, supporting their potential for translational studies. Overall, our results indicated that H 3 PO 4 -Butanol and SPME are the recommended methods for studying SCFAs in fecal samples.
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Food & Function
·
2025
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IF 5.4
Wenxiu Wang,Zhuo Liu,Ying He,Xue Wang,Jiabin Ma,Jingyi Ma,Pengfei Chang,Huidong Huang,Tian Yuan,Rui Guo,Yibing Ning,Zhigang Liu
Infant diarrhea remains a leading cause of morbidity and mortality worldwide. Although human milk oligosaccharides (HMOs) have been clinically linked to reduced pathogen colonization in mice, their preventive effects on infant diarrhea and the underlying mechanisms remain unclear. In this study, one-week-old diarrhea C57BL/6J mice were induced with enteropathogenic Escherichia coli O127 (EPEC O127) to establish an infant diarrhea model. Meanwhile, the mice received HMOs containing 2’-fucosyllactose (2’-FL, 1 g/kg) and/or lacto-N-neotetraose (LNnT, 0.3 g/kg) for three weeks. The results showed that HMOs significantly alleviated diarrhea symptoms, mitigated anxiety-like behaviors, reduced colon inflammation, and restored gut barrier integrity. Notably, HMOs reshaped the gut microbiota by increasing the relative abundance of short-chain fatty acids (SCFAs)-producing bacteria (e.g., Lacrimispora), thereby elevating fecal SCFAs levels while decreasing Escherichia and other harmful taxa. These findings suggest that HMOs can effectively relieve EPEC-induced infant diarrhea by modulating the composition and metabolic function of the gut microbiota. Overall, this study highlights the potential of HMOs as a dietary intervention for alleviating infant diarrhea and provides new insights into their application in infant health.
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