ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Shengyang Pan,Min Chen,Limin Wu
Although self-healing superhydrophobic surfaces have aroused much attention due to their repairable wetting properties and wide applications, the realization of dual healing of both microstructure and surface chemistry remains challenging. Herein, we develop a novel superhydrophobic surface composed of a shape memory micropillar array decorated by pH-responsive capsules encapsulated with fluoroalkylsilane. Owing to the shape memory effect of micropillar and the encapsulated surface chemistry healing agent fluoroalkylsilane, the obtained surface can not only repair the damaged surface chemistry after acid stimuli, but also restore the crushed surface microstructures by heat.
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AICHE JOURNAL
·
2019
·Vol. 66
·期 4
·e16879
·
IF 3.463
Xue-Li Cao,Jia-Lin Guo,Jing Cai,Mei-Ling Liu,Susilo Japip,Weihong Xing,Shi-Peng Sun
The considerable performance enhancement of small molecule-sieving nanofiltration membrane has been achieved by the functional combination between host–guest chemistry and interfacial polymerization (IP) for the first time in this work. First, the water-insolubility of cucurbit[6]uril (CB6) was ameliorated by constructing host–guest complex (CB6-PIP) with piperazine. Second, the incorporation of water-soluble CB6-PIP in the selective layer via IP leads to the generation of not only the enlarged conventional polyamide network tunnels but also rotaxane tunnels. Such enrichment of solvent transport tunnels contributes to an amazing pure water permeability of 15.5–25.4 Lm −2 bar −1 h −1 , three times higher than that of traditional polyamide membranes, with a high R/MgSO 4 of 99.5–92.5%, perfect SO 4 2− /Cl − selectivity due to the electronegative contribution of CB6, as well as untapped potential in organic solvent nanofiltration. This work not only provides a fire-new strategy to design new type of NF materials but also promotes the application of CBs in many other fields.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2019
·
IF 2.931
Yong Wang,Yaxin Mu,Jie Hu,Qianfen Zhuang,Yongnian Ni
In the work, water-soluble bovine serum albumin-protected fluorescent nickel nanoclusters (BSA-NiNCs) are used as fluorescent probes to construct a label-free fluorescence quenching sensor for sensitive and selective detection of tartrazine . The fluorescent BSA-NiNCs are synthesized in one pot using BSA as both the template and reducing agent, and hydrogen peroxide as the additive. The as-prepared NiNCs are characterized by using various analytical techniques like transmission electron microscopy , X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, UV–Vis absorption spectroscopy, circular dichroism spectroscopy, and fluorescence spectroscopy. The synthesized BSA-NiNCs have a quantum yield of ca. 8% by using quinine sulfate as a standard. The sensor for tartrazine detection shows a wide linear range of 0.01–3.5 μM, with a low detection limit of 4 nM. The fluorescence quenching very likely results from the combination of the intermolecular interactions and the secondary inner filter effect between BSA-NiNCs and tartrazine. Then, the proposed sensor is successfully employed for tartrazine detection in drink samples, and the results are comparable with those based on a reference HPLC method.
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LAB ON A CHIP
·
2019
·Vol. 19
·期 5
·845-850
·
IF 6.914
Lei Liu,Mao-Rong Xie,Yu-Zhu Chen,Zhi-Yong Wu
On-line enrichment is effective for improving the sensitivity of paper-based analytical devices (PADs). Electrokinetic stacking of ionic species – anionic or cationic species, respectively, on a paper-based fluidic channel has been well demonstrated in the literature. In this work, we further demonstrated that both anionic and cationic species can be electrokinetically stacked and separated simultaneously on the same paper fluidic channel. The feasibility of the proposed method was visually demonstrated by using a colored cationic probe of Rhodamine 6G and an anionic probe of Brilliant Blue. With the introduction of a background electrolyte (BGE) consisting of weak acid and weak base salt, two electric field gradients can be developed on the same paper fluidic channel when a DC voltage was applied. Both of the anionic and cationic species from the reservoirs can be simultaneously stacked as separate bands on the two field gradients, respectively. Under optimized conditions, two orders of magnitude enrichment factors can be achieved for the anionic and cationic probes as characterized by colorimetric analysis by smartphone imaging. The applicability of this method was further demonstrated by stacking and separation of copper ions/nitrite and even amphoteric ions–proteins of phycocyanin (blue, pI 4.4)/cytochrome C (brown, pI 10.2). Potential applications can be found not only for a PAD based point of care test (POCT), but also for sample pretreatment in protein analysis considering the friendliness of the BGE to the mass spectrometer.
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Nature Communications
·
2019
·Vol. 10
·期 1
·1-11
·
IF 11.878
Yang Lu,Ma Zaijun,Wang Han,Niu Kongyan,Cao Ye,Sun Le,Geng Yang,Yang Bo,Gao Feng,Chen Zuolong,Wu Zhen,Li Qingqing,Shen Yong,Zhang Xumin,Jiang Hong,Chen Yelin,Liu Rui,Liu Nan,Zhang Yaoyang
The long-lived proteome constitutes a pool of exceptionally stable proteins with limited turnover. Previous studies on ubiquitin-mediated protein degradation primarily focused on relatively short-lived proteins; how ubiquitylation modifies the long-lived proteome and its regulatory effect on adult lifespan is unclear. Here we profile the age-dependent dynamics of long-lived proteomes in Drosophila by mass spectrometry using stable isotope switching coupled with antibody-enriched ubiquitylome analysis. Our data describe landscapes of long-lived proteins in somatic and reproductive tissues of Drosophila during adult lifespan, and reveal a preferential ubiquitylation of older long-lived proteins. We identify an age-modulated increase of ubiquitylation on long-lived histone 2A protein in Drosophila , which is evolutionarily conserved in mouse, monkey, and human. A reduction of ubiquitylated histone 2A in mutant flies is associated with longevity and healthy lifespan. Together, our data reveal an evolutionarily conserved biomarker of aging that links epigenetic modulation of the long-lived histone protein to lifespan.
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Viruses-Basel
·
2019
·Vol. 11
·期 4
·314
·
IF 3.811
Gaobo Zhang,Jian Yang,Fujun Qin,Congrui Xu,Jia Wang,Chengfeng Lei,Jia Hu,Xiulian Sun
Dendrolimus punctatuscypovirus (DpCPV), belonging to the genusCypoviruswithin the familyReoviridae, is considered the most destructive pest of pine forests worldwide. DpCPV has a genome consisting of 10 linear double-stranded RNA segments. To establish a reverse genetics system, we cloned cDNAs encoding the 10 genomic segments of DpCPV into three reverse genetics vectors in which each segment was transcribed under the control of a T7 RNA polymerase promoter and terminator tagged with a hepatitis delta virus ribozyme sequence. We also constructed avp80-knockoutAutographa californicamultiple nucleopolyhedrovirus bacmid to express a T7 RNA polymerase codon-optimized for Sf9 cells. Following transfection of Sf9 cells with the three vectors and the bacmid, occlusion bodies (OBs) with the typical morphology of cypovirus polyhedra were observed by optical microscopy. The rescue system was verified by incorporation of aHindIII restriction enzyme site null mutant of the 9th genomic segment. Furthermore, when we co-transfected Sf9 cells with the reverse genetics vectors, the bacmid, and an additional vector bearing anegfpgene flanked with the 5′ and 3′ untranslated regions of the 10th genomic segment, aggregated green fluorescence co-localizing with the OBs was observed. The rescued OBs were able to infectSpodopetra exigualarvae, although their infectivity was significantly lower than that of wild-type DpCPV. This reverse genetics system for DpCPV could be used to explore viral replication and pathogenesis and to facilitate the development of novel bio-insecticides and expression systems for exogenous proteins.Keywords:DpCPV;reverse genetics system;vp80-knockout;foreign protein
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RSC Advances
·
2019
·Vol. 9
·期 45
·26165-26171
·
IF 3.049
Shengyang Pan,Min Chen,Limin Wu
Although superomniphobic surfaces have attracted extensive interest owing to many important applications, successful fabrication of such surfaces still remains a critical challenge. Herein, we present a flexible transparent superomniphobic polydimethylsiloxane (PDMS) surface with a micropillar array using Si nanowires as the mould. The as-obtained PDMS not only exhibits excellent liquid-repellent performance with a static contact angle of over 150° and sliding angle of less than 6° against a wide range of liquids, but also maintains the super-repellency even under acid/base corrosion, mechanical damage, and unidirectional stretching.
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CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Yiheng Song,Jisuan Tan,Guan Wang,Li Zhou
Herein, we demonstrated the potential of peach gum polysaccharide-based amine-rich gel (ARG) as an efficient adsorbent for removal of anionic dyes from water. The adsorption performance of ARG was systematically studied by choosing methyl orange (MO) and amaranth (ART) as representative anionic dyes. The effects of various parameters such as pH, ionic strength, temperature, initial dye concentration and contact time on the adsorption were investigated. The ARG exhibited superior adsorption selectivity and stable adsorption behaviors against variation of pH and ionic strength for anionic dyes. Adsorption process reached equilibrium within 10 min and showed good correlation with pseudo-second-order kinetic model and Langmuir isotherm. The adsorption capacity of ARG for MO and ART can reach 1949.5 and 1082.2 mg g −1 , respectively. Based on its sustainable characteristic, low cost and excellent adsorption property, the ARG holds great promise for utilizing as an adsorbent for practical water treatment applications.
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FOOD CHEMISTRY
·
2018
·
IF 4.946
Tong Bu,Qiong Huang,Lingzhi Yan,Wentao Zhang,Leina Dou,Lunjie Huang,Qingfeng Yang,Bingxin Zhao,Baowei Yang,Tao Li,Jianlong Wang,Daohong Zhang
Here, a facile, label-free and sensitive lateral flow strip (LFS) biosensor for foodborne pathogens was established relying on the innovative introduction of Gram staining and the direct immunoreaction. Target bacteria can be directly marked with crystal violet (CV) by one-step staining which is superior to traditional signal marking techniques in LFS assay, and the method’s selectivity can be guaranteed by high-specificity monoclonal antibody. With Salmonella Enteritidis ( S. Enteritidis) as a model target, this protocol can selectively detect 80 CFU mL −1 S. Enteritidis within 11 min in the optimized conditions. Moreover, with Listeria monocytogenes as another model target, the biosensor shows a high universality for detections of both gram-negative and gram-positive bacteria. The unexpected applicability of biological dye tracer in strip biosensor reveals that the biological dye can be a potential tool serving as a universal signal tracer for pathogenic microorganisms in food safety monitoring and early clinical diagnosis.
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BIOMEDICAL MICRODEVICES
·
2018
·Vol. 20
·期 3
·1-8
·
IF 2.077
Wang Tengfei,Xu Guangning,Wu Wenyuan,Wang Xiaoying,Chen Xueliang,Zhou Shumin,You Fan
Nowadays, there is great interest in the use of microfluidic paper-based analytical devices (μPADs) for low-cost diagnostics. In most cases, the test equipment is needed. Here we report a new type of device-independent detection method based on timer-paper-based analytical devices, which can be tested by smartphone, and its application for cholesterol detection. The Quick Response code was designed as the timer component of this device. Wax printing method was employed to print the pattern on filter paper. The color and enzyme reagents have been immobilized in the hydrophilic channel to complete the colorimetric detection for cholesterol. Under laminar flow conditions of the cellulose network, the liquid volume of detection zone has been quantified by monitoring the fluid residence time on different area of the timer-paper-based devices. The precise monitoring of detection time can promote the accuracy of colorimetric detection. This is very important for the quantitative detection of paper-based analytical devices. One significant outcome of this report is that simple and accurate timer can be used for detection process self-clocking. The factors of total detection time have been investigated. The linear range of the calibration curve was 3.0 ~ 6.0 mmol L −1 , with correlation coefficient of 0.9956. With the characteristic of easy to use, low cost and accurate monitoring of detection time, this kind of timer-paper-based devices can be applied to cholesterol or other substances rapid detection.
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Food Analytical Methods
·
2018
·Vol. 12
·期 2
·381-393
·
IF 2.245
Sun Xiao-Dong,Wu Hai-Long,Liu Zhi,Chen Yue,Liu Qian,Ding Yu-Jie,Yu Ru-Qin
A rapid and sensitive analytical strategy combining high-performance liquid chromatography-diode array detection (HPLC-DAD) and chemometrics methods was developed for the determination of multi-class food additives in a variety of beverage samples. Different kinds of beverages, which contain diverse unknown interferences, can be directly injected into a chromatographic system after a simple dilution or pretreatment step, and the data were recorded in a short time under a simple gradient elution mode. Although peaks overlapped and changeable interferences existed in the dimension of the chromatographic and spectral in real beverages, all food additives were accurately resolved by using a second-order calibration method based on alternating trilinear decomposition (ATLD) algorithm, and accurate chromatographic profiles, spectral profiles, and concentration profiles were also obtained. Limits of detection for all additives were between 1.40 and 165.1 ng mL −1 , while limits of quantitation ranged from 4.20 to 500.2 ng mL −1 . The spiked recoveries were in the range of 87.3–103% (except amaranth) with RSDs less than 10.2%. Compared with the results obtained by classic HPLC method, our proposed method was more accurate. In all, the proposed method is fast, sensitive, and universal and could be used as a reliable tool to determine food additives and quality monitoring in different complex beverages.
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ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Bin Shang,Min Chen,Limin Wu
The biomimetic fabrication of Nepenthes as a new class of liquid-repellent surface materials has attracted much attention recently, but there still remains big challenges, for example, short-serving life, instability, and so forth. Herein, we develop a novel strategy to fabricate robust slippery liquid-repellent coating by the synthesis and use of silicon oil-contained ultraviolet-responsive microcapsules. Owing to the physical and chemical inertness of silicon oil and the substantial randomly dispersed microcapsules in the coating, this nepenthes-like liquid-repellent coating can not only endure different harsh environments but also have excellent long-term self-healing functions.
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危险品化学品经营许可证（带存储）