DYES AND PIGMENTS
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2022
·
IF 5.122
Lingfei Liu,Minjie Qian,Zhen-quan Yang,Lixia Xiao,Xiaojuan Gong,Qin Hu
In this work, a facial ratiometric fluorescence nanorpobe (RFP) based on gadolinium and nitrogen co-doped CDs ( Gd , N -CDs) was for the first time proposed for visual detection of folic acid (FA). The Gd , N -CDs were prepared by hydrothermal heating of Gd(NO 3 ) 3 ⋅6H 2 O, o-phenylenediamine (OPD), 4-aminobenzoic acid (PABA). The as-prepared Gd , N -CDs had an average particle size of 10.27 ± 0.35 nm and exhibited two emission bands at 396 and 548 nm, respectively upon excitation at 340 nm. In the presence of FA, the 560 nm emission of Gd , N -CDs was reduced while the fluorescence signal at 457 nm was enhanced, and the quantitatively determination of FA was based on the fluorescence intensity ratio (F 457 /F 560 ). The as-constructed RFP achieved a linear range of 0.0–30.0 μM and a low detection limit (LOD) of 3.43 nM. In particular, a distinguishable change in fluorescence colour from bright orange to blue enabled the development of a smartphone-assisted visual sensing platform and a paper-based analytical device for FA detection with LODs of 3.06 μM and 0.83 μM, respectively. The proposed sensing platform was successfully utilised for onsite detection of FA in vegetable, fruit and yolk samples with satisfactory results were achieved. This work highlights the application potential of smartphone-integrated CDs-based RFP as a promising portable method for on-site analysis of FA.
研究领域:
分析化学
·
生物医学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2018
·
IF 2.88
Fengyuan Zhang,Xinyue Zhu,Zhijuan Jiao,Xiaoyan Liu,Haixia Zhang
An uncontrolled increase of nitrite concentration in groundwater, rivers and lakes is a growing threat to public health and environment. It is important to monitor the nitrite levels in water and clinical diagnosis. Herein, we developed a switch-off fluorescence probe (PyI) for the sensitive detection of nitrite ions in the aqueous media. This probe selectively recognizes nitrite ions through a distinct visual color change from colorless to pink with a detection limit of 0.1 μM. This method has been successfully applied to the determination of nitrites in tap water, lake water and Yellow River water with recoveries in the range of 94.8%–105.4%.
研究领域:
环境科学
·
分析化学
·
公共健康
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2018
·
IF 3.129
Kaiqiang Wang,Da-Wen Sun,Qingyi Wei,Hongbin Pu
Emulsion is a commonly investigated bioactive loaded delivery system. The bioactive content and its location in oil phase primarily determine the quality and chemical stability of emulsion. In this study, Raman microspectroscopy was used to quantify α-tocopherol and to visualize its distribution in oil-in-water emulsion stabilized by whey protein isolates. Results suggested that α-tocopherol contents (25–300 g/kg) in corn oil with the integrating Raman intensity at 481.9 and 588.6 cm −1 showed determination coefficient ( R 2 ) of 0.98 and 0.99, and limit of detection of 5.1 and 21.2 g/kg, respectively. For detecting α-tocopherol in emulsions, the relative standard deviation values from Raman method using intensities at 481.9 cm −1 and 588.6 cm −1 were in the ranges of 4%–16% and 2%–6%, respectively. The developed Raman method provided correlative results with those of HPLC method ( R 2 = 0.99). Moreover, Raman chemical imaging depicted the non-homogeneous TOC distribution within oil droplets, where TOC had the trend of migrating to the interface of oil and water. This study provided a novel approach for functional emulsion analysis, which may serve the basis for designing stable and controllable release of emulsion systems in future.
研究领域:
食品科学
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分析化学
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产品关联
DOI
高影响因子
TALANTA
·
2017
·
IF 4.162
Weijie He,Rijun Gui,Hui Jin,Benqian Wang,Xiangning Bu,Yongxin Fu
In this article, blue-emitting carbon dots (CDs) were prepared via hydrothermal treatment of sodium citrate and NH 4 HCO 3 , and then combined with 3-aminophenylboronic acid (APBA) to prepare APBA modified-CDs. APBA acted as the receptor of dopamine (DA). Using bovine serum albumin (BSA) as a stabilizer and N 2 H 4 ·H 2 O as a reducing reagent, BSA-stabilized and red-emitting copper nanoclusters (CuNCs) were prepared. By carbodiimide-activated coupling, novel nanohybrids consisting of CDs and CuNCs were constructed and exhibited dual-emitting fluorescence (FL). In the presence of DA, marked FL (at 440 nm) quenching of nanohybrids was detected. Specific coupling interactions between boric acid of APBA and cis-glycol of DA induced the combination of DA and APBA on the surface of CDs. As a superior electron receptor, DA triggered the electron transfer from CDs to DA, resulting in the FL quenching of CDs in nanohybrids. The FL (at 640 nm) of CuNCs in nanohybrids was almost unchanged after the addition of DA, and so further used for a reference FL to develop a novel ratiometric FL probe for DA detection. In addition to high sensitivity and selectivity, superior analytical performances of this probe were confirmed in applications, including dual-signal FL sensing of DA and naked-eye visual FL imaging of DA in aqueous solution and on filter paper.
研究领域:
分析化学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2017
·Vol. 409
·期 28
·6655-6662
·
IF 3.431
Li Hongmei,Wang Xuefeng,Cai Zheng,Lu Ling,Tao Jia,Sun Bin,Yang Yuanyuan,Xu Quan,Yu Zhiqiang,Zhao Peng
Copper ions play a significant role in human bodies, as they are beneficial for many metabolism processes, but excessive Cu 2+ may cause various symptoms. Therefore, the development of strategies capable of both sensitive detection and visualization of Cu 2+ is greatly important for human health. In this work, a novel ratiometric fluorescence sensor for rapid and visual detection of Cu 2+ was designed based on green emission carbon dots (CDs) and red emission CdTe/CdS quantum dots (QDs). We have calculated the chromaticity coordinates of individual CDs and CdTe/CdS QDs, and the chromaticity coordinate of their mixture would change along the line between the two points of CDs and CdTe/CdS QDs in the chromaticity coordinate system. The red fluorescence of QDs can be influenced by Cu 2+ , and the fluorescence color of CDs-QDs changes from yellow to green as the chromaticity coordinates change with the addition of Cu 2+ . This traffic-light-type color change was more sensitive to the naked eye compared to conventional single emission-based sensors. This paper not only realized ratiometric fluorescence and visual detection of Cu 2+ through a convenient method but also provided a promising strategy of designing an assay kit for trace Cu 2+ .
Ratiometric fluorescent sensing of copper ion based on chromaticity change strategy
研究领域:
分析化学
·
生物医学
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产品关联
DOI
PubMed
高影响因子
MATERIALS & DESIGN
·
2025
·
IF 7.9
Yu Ma,Bin Zhao,Guozhen Yan,Ting Zhou,Zhihua Xu,Zhihan Niu,Zhenghong Xu,Tongtong Zhang,Feng Shi
Nucleic acid dyes play important roles in the quantification and detection of nucleic acids by binding to nucleic acids. However, traditional nucleic acid dyes pose great environmental and economic challenges due to their high price, high toxicity, long dyeing time, low sensitivity and insufficient stability. To overcome these problems, we developed a new nucleic acid dye based on carbon dots, which were generated through hydrothermal synthesis with liquorice components as precursors and a system containing ethylenediamine. This new type of carbon dot can effectively replace traditional dyes and can be used to efficiently visualize DNA, RNA and plasmids via agarose gel electrophoresis. Then, we studied the interaction mechanism between carbon dots and nucleic acids by UV–visible spectroscopy, Fourier transform infrared spectroscopy and circular dichroism spectroscopy. The interaction between the carbon dots and nucleic acids mainly occurred through groove binding and was accompanied by a slight electrostatic interaction. Carbon dots-based nucleic acid dyes, with their low cost, simple synthesis process and high stability, have opened a new path for the field of nucleic acid detection. Its research and development not only promote the frontier progress of biological science and medicine but also introduces innovative strategies and tools for scientific research and clinical practice.
研究领域:
生物化学
·
分子生物学
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产品关联
DOI
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 28
·3570-3577
·
IF 2.378
Baoya Zhu,Mingyu Tang,Liying Yu,Yaoyao Qu,Fang Chai,Lihua Chen,Hongbo Wu
In this paper, silicon nanoparticles (Si NPs) were synthesized by a simple one-step procedure by applying uric acid as reducing agent. The as-prepared Si NPs exhibited emission at 425 nm under excitation at 345 nm, allowing them to be developed as a fluorescent and colorimetric probe for detection of Cu2+ and Mn2+. As a probe, the fluorescence and color change of the Si NPs induced by Cu2+ and Mn2+ exhibited contrast. As a fluorescence probe, the Si NPs exhibited intense enhancement caused by Cu2+ and Mn2+ with detection limits of 0.562 μM (Cu2+) and 1.02 μM (Mn2+). More significantly, the Si NPs could be applied to effectively detect Cu2+ and Mn2+ in real samples. Interestingly, a Si NPs-based gel membrane was fabricated and established to be selective for visual recognition of Cu2+ and Mn2+. A cell imaging experiment revealed that the Si NPs possessed excellent biocompatibility and low cytotoxicity in living cells. Additionally, the powder form of the Si NPs can be easily prepared and used to visualize latent fingerprints (LFPs) with good performance.
研究领域:
分析化学
·
材料科学
·
生物医学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Fang Zhou,Yasmina Sultanbawa,Huan Feng,Yong-Lei Wang,Qingtao Meng,Yue Wang,Zhiqiang Zhang,Run Zhang
The development of new methods for rapid and effective detection of bisulfite (HSO3–) in food samples and imaging of HSO3– intake in animals is of significant importance due to the key roles of HSO3– in food quality assurance and community health. In this work, a new responsive fluorescence probe, EQC, is reported for the quantitative detection of HSO3– in food samples and visualization of HSO3– intake in animals. Upon addition of HSO3–, the UV–vis absorption and red emission of EQC were significantly decreased within 120 s. The changes in absorption and emission spectra of EQC were rationalized by theoretical computations. The proposed reaction mechanism of EQC with HSO3– was confirmed by high-resolution mass spectrometry (HRMS) and spectroscopic titration measurements. EQC has the advantages of high sensitivity, selectivity (a detection limit of 18.1 nM), and fast response toward HSO3–, which enable rapid and effective HSO3– detection in buffer solution. The practical applications of EQC were demonstrated by the detection of HSO3– in food samples and the imaging of HSO3– intake in live animals.
研究领域:
分析化学
·
食品安全
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Applied Materials Today
·
2021
·
IF 10.041
Juan Liu,Zhigang Chen,Chengshen Hu,Wei Yang,Jinfeng Wang,Weilong Xu,Yuanliang Wang,Changshun Ruan,Yanfeng Luo
Shape memory polyurethanes (SMPUs) with excellent shape memory effects (SMEs) can be served as a kind of smart materials capable of responding to external stimuli. Microphase separation behavior between soft and hard segments of SMPUs is recognized as structural basis and an intrinsic factor for SMEs of SMPUs. Therefore, effective detection of microphase separation behavior is highly desired in order to clarify the mechanism of SMEs and further regulate the SMEs of SMPUs. Although great progresses have been made in detection of microphase separation behavior of SMPUs, non-destruction and real-time visualization for tracking the microphase separation behavior during the shape memory process of SMPUs remains a great challenge. Herein, a facile fluorescence visualization method is presented to directly, fast and nondestructively monitor the microphase separation behavior of SMPUs. Specifically, a series of novel inherent fluorescent SMPUs (IFPUs) with different hard segment contents (10%, 15% and 20%) were first prepared by using a fluorescent small molecule as part of their hard segments. It was found that this fluorescent molecule could visualize the aggregation of hard segments and the corresponding microphase separation behavior in a real-time manner. Moreover, by conducting a semi-quantitative calculation of the collected fluorescent images, the size, aspect ratio and orientation of the hard segment domains could be obtained. The results indicated that increasing the contents of hard segments could enhance the microphase separation and both stretching (50%, 100% and 200%) and subsequent recovering processes could promote the formation of larger and longer hard segment domains tending to take a perpendicular orientation to stretching.
研究领域:
材料科学
·
高分子化学
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产品关联
DOI
高影响因子
Food Analytical Methods
·
2021
·Vol. 14
·期 8
·1637-1644
·
IF 3.366
He Yu,Wang Shuo,Wang Junping
Folic acid (FA) is a vitamin essential for human physiology; thus, rapid, accurate, and sensitive methods for its detection and quantification in different biological samples are necessary. A fluorescent ratiometric nanoprobe (the complex composed of quantum dots and carbon dots) based on carboxyl-modified quantum dots (QDs) and amino-modified graphene-based carbon dots (CDs) has been developed for the visual determination of folic acid (FA). The nanoprobe is the composition in which QDs were covalently bonded with CDs. Red and blue fluorescence of nanoprobe can be selectively quenched and enhanced by FA, respectively. Thus, the nanoprobe is capable to generate clearly visible fluorescence color change from pink to blue upon exposure to UV light. Depending on the color change and its intensity, FA can be identified, and their concentrations can be determined. The sensor prepared based on the nanoprobe demonstrated good selectivity and sensitivity toward FA with exceptionally low detection limit equal to 0.039 μM. We also prepared a paper-based sensor to demonstrate convenient and simple approach to visually detect FA. Thus, the nanoprobe demonstrated feasibility of rapid real-time quantitative FA detection; considering its simple, effective, and practicality, expectedly it can be used for practical application.
研究领域:
分析化学
·
生物医学工程
·
食品科学
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 24
·19771-19778
·
IF 3.201
Yunsuo Kuang,Linfeng Chen,Jiahui Lu,Xike Tian,Chao Yang,Yong Li,Liqiang Lu,Yulun Nie
In this work, we successfully developed an ultrasensitive dual-emission fluorescent sensor (CRB) for instant visual determination of Cu2+ by modifying a Cu-sensitive rhodamine B derivative (RB) onto photostable carboxyl modified carbon dots (CDs). The RB moiety of the CRB was extremely sensitive to Cu2+ and exhibited significant orange fluorescence in 1 second, while the blue CD moiety was stable to Cu2+. As a result, significant fluorescent color transformation from blue to orange could be observed in the presence of Cu2+, which could be applied to rapid visualization of Cu2+. The CRB exhibited good selectivity and excellent sensitivity to Cu2+, and the detection limit (LOD) was estimated to be 2 ppb. Moreover, a portable test strip has been successfully developed and applied for the visual inspection of Cu2+, which provides a convenient and reliable approach to monitor Cu2+ on site without resorting to instrumental analysis.
研究领域:
分析化学
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2014
·
IF 5.9
Zhiyang Zhang,Zhaopeng Chen,Shasha Wang,Chengli Qu,Lingxin Chen
We have described a simple and low-cost visual method for on-site detection of hydrogen sulfide (H2S) in air based on the antiaggregation of gold nanoparticles (AuNPs). The bubbling of H2S into a weak alkaline buffer solution leads to the formation of HS–, which can stabilize the AuNPs and ensure the AuNPs maintain their red color even in a Tris buffer solution containing 80 mM NaCl with the presence of Tween 80. The stabilization of the AuNPs is attributed to the adsorption of negatively charged S2– on the AuNPs surface. In contrast, without the bubbling of H2S, AuNPs aggregate and change color from red to blue. Under optimal conditions, the proposed method exhibits excellent visual sensitivity with a naked-eye detectable limit of 0.5 ppm (v/v), making the on-site detection of H2S possible. This method also possesses good selectivity toward H2S over other gases by using a simple SO2 removal device. The successful determination of the concentrations of H2S in local air indicates the potential application of this cost-effective method.
研究领域:
分析化学
·
环境科学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）