BIOMACROMOLECULES
·
2023
·
IF 6.2
Hanchen Wei,Caiyun Yang,Feihu Bi,Bang Li,Rui Xie,Deshui Yu,Shuzhen Fang,Zan Hua,Qingqing Wang,Guang Yang
Glycopolymer-supported silver nanoparticles (AgNPs) have demonstrated a promising alternative to antibiotics for the treatment of multidrug-resistant bacteria-infected diseases. In this contribution, we report a class of biohybrid glycopolymersome-supported AgNPs, which are capable of effectively killing multidrug-resistant bacteria and disrupting related biofilms. First of all, glycopolymersomes with controllable structures were massively fabricated through reversible addition–fragmentation chain transfer (RAFT) polymerization-induced self-assembly (PISA) in an aqueous solution driven by complementary hydrogen bonding interaction between the pyridine and amide groups of N-(2-methylpyridine)-acrylamide (MPA) monomers. Subsequently, Ag+ captured by glycopolymersomes through the coordination between pyridine-N and Ag+ was reduced into AgNPs stabilized by glycopolymersomes upon addition of the NaBH4 reducing agent, leading to the formation of the glycopolymersome@AgNPs biohybrid. As a result, they showed a wide-spectrum and enhanced removal of multidrug-resistant bacteria and biofilms compared to naked AgNPs due to the easier adhesion onto the bacterial surface and diffusion into biofilms through the specific protein–carbohydrate recognition. Moreover, the in vivo results revealed that the obtained biohybrid glycopolymersomes not only demonstrated an effective treatment for inhibiting the cariogenic bacteria but also were able to repair the demineralization of caries via accumulating Ca2+ through the recognition between carbohydrates and Ca2+. Furthermore, glycopolymersomes@AgNPs showed quite low in vitro hemolysis and cytotoxicity and almost negligible acute toxicity in vivo. Overall, this type of biohybrid glycopolymersome@AgNPs nanomaterial provides a new avenue for enhanced antibacterial and antibiofilm activities and the effective treatment of oral microbial-infected diseases.
研究领域:
材料科学
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纳米技术
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生物医学工程
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微生物学
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
·
2024
·
IF 4.4
Qincheng Ma,Honglin Zhou,Xiang Shao,Junbao Chen,Yujie Cheng,Mengxin Chen,Yizhang Jiang,Xiangxiang Chen,Qingping Ke,Ying Wang,Jun Tang,Yunyuan Dong,Ping Cui
Liquid–liquid biphasic reactions are widely used in academic research and industrial production; however, they have always suffered from low reactivity. In this work, a variety of linear polymeric catalysts were prepared by reversible addition–fragmentation chain transfer polymerization and postoxidation steps. The amphiphilicity of polymeric catalysts was imparted by varying the addition ratios of hydrophilic 1,1,3,3-tetramethyl-2-(4-vinylbenzyl) guanidine, hydrophobic styrene, and catalyst precursor 2,2,6,6-tetramethyl-4-piperidyl methacrylate, thus forming stable Pickering emulsions. Such Pickering emulsion systems with an abundant interfacial area and a unique interfacial environment can promote the selective oxidation reaction. As exemplified by 2,2,6,6-tetramethylpiperidinooxy-mediated oxidation of cinnamyl alcohol, the selectivity of cinnamaldehyde in Pickering emulsions was 97.2% with a turnover frequency of 5.0 mol·mol–1·min–1, which is higher than reported results. The carbon dioxide responsiveness of the guanidine group on the polymers allowed for fast demulsification after the reaction, and the recycled polymer maintained high activity after 10 runs. This polymer-stabilized Pickering emulsion provides a valuable pathway for efficient green catalytic oxidation reactions.
研究领域:
化学工程
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材料科学
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催化
·
绿色化学
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN POLYMER JOURNAL
·
2022
·
IF 5.546
Yaotian Peng,Xiaoyan Feng,Jianming Jiang,Li Ren
Bilirubin, a dangerous toxin may lead to serious brain injury, can be widely removed by hemoperfusion. However, the hemoperfusion adsorbents with higher removal ability, excellent hemocompatibility and biocompatibility still need to be explored. In this work, a novel functionalized polyvinylpyrrolidone (PVP) was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization. Then PVP-modified polystyrene-divinylbenzene (PS-DVB-PVP) microspheres were prepared by a one-pot restore-click method. The results showed that the adsorption capacity and rate of microspheres for bilirubin were increased after modification with PVP. In the hemocompatibility and biocompatibility test, the modified microspheres demonstrated higher resistance to human serum albumin (HSA) and excellent hemolysis rate. In order to study the role of PVP in the bilirubin-adsorption process, isotherms were fitted by Langmuir model and Freundlich model, while kinetics data were fitted by pseudo-second-order model and intraparticle diffusion model. In addition, the modified microspheres showed no difference with unmodified microspheres in the coagulation test and exhibited no cytotoxicity to L929 cells. These results showed that our modified microspheres have both excellent hemocompatibility and outstanding biocompatibility, which will not cause hemolysis and coagulation. All these results made them possible to become a new adsorbent for efficient removing bilirubin in hemoperfusion.
研究领域:
材料科学
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生物医学工程
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化学工程
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
Acta Biomaterialia
·
2014
·
IF 5.684
Junmin Qian,Minghui Xu,Aili Suo,Weijun Xu,Ting Liu,Xuefeng Liu,Yu Yao,Hongjie Wang
To minimize the side effects and enhance the efficiency of chemotherapy, a novel folate-decorated hydrophilic cationic star-block terpolymer , [poly( l -glutamic acid γ-hydrazide)- b -poly(N,N-dimethylaminopropyl methacrylamide)] 3 - g -poly(ethylene glycol) ((PGAH- b -PDMAPMA) 3 - g -PEG), with disulfide linkages between the PEG and PDMAPMA blocks, was developed for targeted co-delivery of doxorubicin and Bcl-2 small interfering RNA (siRNA) into breast cancer cells. The terpolymer was synthesized by a combination of ring-opening polymerization, reversible addition–fragmentation chain transfer polymerization, PEGylation and hydrazinolysis. The chemical structures of the polymers were confirmed by 1 H-NMR analysis. The terpolymer could conjugate doxorubicin via an acid-labile hydrazone linkage and simultaneously efficiently complex siRNA through electrostatic interaction at N/P ratios of ⩾4:1 to form “two-in-one” nanomicelleplexes, which displayed a spherical shape and had an average size of 101.3 nm. The doxorubicin loading efficiency and content were 61.0 and 13.23%, respectively. The cytotoxicity, drug release profile, targeting ability, cellular uptake and intracellular distribution of the nanomicelleplexes were evaluated in vitro. We found that the release behaviors of doxorubicin and siRNA had a pH/reduction dual dependency. They were released faster under reductive acidic conditions (pH 5.0, glutathione : 10 mM) than under physiological conditions (pH 7.4). The folate-decorated nanomicelleplexes could deliver doxorubicin and Bcl-2 siRNA more efficiently into the same MCF-7 cell and exhibited a higher cytotoxicity than non-targeted nanomicelleplexes. These results indicate that the terpolymer could act as an efficient vehicle for targeted intracellular co-delivery of doxorubicin and therapeutic siRNA in cancer therapy.
研究领域:
药学
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纳米技术
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癌症治疗
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高分子化学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
IRANIAN POLYMER JOURNAL
·
2025
·
IF 2.8
Dai Yunling,Ma Xinyu,Du Pan,Lin Qiurui,Zhang Wei,Yang Yining,He Xianru
Polymers with lower critical solution temperature (LCST) had broad application prospects in biomedicine and drug delivery. The copolymer poly(N-vinylpyrrolidone-co-diacetone acrylamide) (P(NVP-co-DAAM)) was prepared by reversible addition-fragmentation chain transfer (RAFT) polymerization of N-vinylpyrrolidone (NVP) and diacetone acrylamide (DAAM). Ultraviolet transmittance was utilized to investigate its solution behavior. The results showed that by adjusting molar ratio of two monomers, the lower critical solution temperature (LCST) of the copolymer could be modulated for 12.0–59.9 °C. Variable-temperature Fourier transform infrared spectroscopy examined the interactions between the copolymer and water molecules. The results indicated that the hydroxyl absorption peak of the solvent water molecules exhibited blue-shift with rising temperature, indicating weakening interactions between copolymer and water molecules. Two-dimensional infrared correlation spectroscopy results showed that interaction between water molecular and DAAM structural unit weakened prior to that between water molecular and NVP structural unit due to rising temperature. The concentration dependence of the increasing specific viscosity indicated that the aggregation concentration (Cg) of the polymer solution decreased with increasing temperature.Dynamic light scattering experiments showed that the hydrodynamic size increases with increasing temperature above the LCST. The results comprehensively showed that the P(NVP- co -DAAM) copolymer exhibits a sensitive temperature response, and the P(NVP- co -DAAM) structure has good biocompatibility, indicating its significant potential for application in biomedicine and related fields. Graphical
研究领域:
高分子科学
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材料科学
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生物医学工程
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药物输送
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物理化学
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
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2025
·
IF 19
Qian Li,Tong Wang,Qu Wang,Kaihao Wang,Li Wang,Wantai Yang
Engineering advanced thermochromic smart windows capable of simultaneously addressing multifaceted performance, durability, and multifunctionality requirements is crucial for their practical applications. Herein, a mini library of lower critical solution temperature (LCST) copolymers with well-defined structure and programmed hydrophile-lipophile balance (HLB) is synthesized by the reversible addition-fragmentation chain transfer (RAFT) copolymerization of highly hydrophilic N-vinylpyrrolidone (NVP) and four maleimide derivative (MI) monomers with descending hydrophilicity. MI-NVP copolymers afford a copolymer composition-dependent phase transition temperature tunable over an ultra-wide range of 20–67 °C and efficient UV shielding capability. By ingeniously doping MI-NVP copolymers into non-thermoresponsive polyacrylamide hydrogel, smart windows with outstanding thermochromic properties, solar modulation performance, inherent stability (1000 cycles), and fast response rate (14 ± 2 s) are obtained. Further integration with photothermal Cs x WO 3 or electrothermal indium tin oxide (ITO) glass delivers enhanced spectral selectivity or active-mode regulation. Overall, by meticulously tailoring the multilevel structure of thermochromic hydrogels, this strategy establishes a novel MI-NVP copolymer platform for thermochromic smart windows that significantly advances energy conservation and occupant comfort.
研究领域:
材料科学
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高分子化学
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智能窗户
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建筑节能
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功能高分子
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光学材料
✓ 已验证
产品关联
DOI
高影响因子
POLYMER
·
2011
·
IF 3.829
Zhuan Yi,Liping Zhu,Liang Cheng,Baoku Zhu,Youyi Xu
An amino-substituted polyethersulfone (PES) was synthesized by the polycondensation of a functional monomer bis(3-amino-4-hydroxyphenyl) sulfone with bis(4-fluorophenyl) sulfone. The amine groups incorporated into PES were employed as anchors to immobilize the chain transfer agents of reversible addition-fragmentation polymerization (RAFT). The resultant macro chain transfer agent was used to initiate the polymerization of the hydrophilic monomers N -isopropyl acrylamino (NIPAAm) and N, N -dimethylamino-2-ethyl methacrylate (DMAEMA), respectively. The gel permeation chromatography (GPC) results confirmed the successful synthesis of the amphiphilic copolymers PES- g -PNIPAAm and PES- g -PDMAEMA, and these two copolymers were perhaps the few examples of amphiphilic copolymer synthesized via a radical polymerization from PES main chains. The amino-substituted PES seemed a versatile precursor that showed a potential of functionalization via various strategies including click chemistry, atom transfer radical polymerization and RAFT polymerization. The synthesized amphiphilic copolymers were finally used as additives to improve the hydrophilicity and the filtration performances of PES membranes.
研究领域:
高分子化学
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材料科学
·
膜技术
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Polymeric Materials and Polymeric Biomaterials
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2022
·
IF 3.221
Zhao Wang,Wenli Song,Ruilong Sheng,Xinyu Guo,Lingyun Hao,Xiaojuan Zhang
A new series of cholesterol-bearing poly(methacrylate)s with pendant cationic (l-)-lysine (Chol-PHML) was synthesized via reversible addition–fragmentation chain transfer (RAFT) polymerization, followed by Boc-deprotection. Their structures were characterized by nuclear magnetic resonance (NMR) spectroscopy and gel permeation chromatography (GPC). Then, the average particle sizes and zeta potentials of the Chol-PHML/pDNA polyplexes were examined using dynamic light scattering (DLS), and their plasmid DNA (pDNA)-binding ability was analyzed using agarose gel retardation assay. In vitro cytotoxicity of the polycations was determined using a cell counting kit-8 (CCK-8) assay and lactate dehydrogenase (LDH) kits in HeLa cells. The polyplex’s gene transfection effectiveness was investigated using a luciferase assay. The results revealed that the Chol-PHML series could efficiently bind/load pDNA with a high binding affinity (N/P < 3.0) and subsequently form nanoparticles with the sizes of 90–200 nm and zeta potentials of ∼+40 mV in an aqueous solution. The in vitro cytotoxicity and gene transfection efficiency considerably depended on the Chol-PHML vector’s molecular weight. The optimal Chol-PHML27 exhibited a substantially higher gene transfection efficiency and lower cytotoxicity than those of the linear structural PLL (15 K–30K) and PHML26 control. This study presents a simple method for synthesizing biocompatible and controllable cationic gene vectors by introducing hydrophobic cholesterol moieties, which will function as a promising foundation for gene delivery.
研究领域:
高分子化学
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基因治疗
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生物医学工程
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分子生物学
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2016
·Vol. 40
·期 10
·8397-8407
·
IF 3.277
Beibei Lu,Lei Li,Jianning Wu,Lulu Wei,Jun Hou,Zhiyong Liu,Xuhong Guo
Well-defined dual pH and temperature responsive triblock star-shaped amphiphilic copolymers of β-cyclodextrin-g-poly(2-hydroxyethylmethacrylate)-b-poly(N-isopropylacrylamide)-b-poly(2-(dimethylamino)ethylmethacrylate) (β-CD-g-(PHEMA-b-PNIPAM-b-PDMAEMA)) were synthesized by the combination of reversible addition–fragmentation chain transfer (RAFT) polymerizations. The chemical structures and compositions of these copolymers were characterized by Fourier transform infrared spectroscopy (FTIR), proton nuclear magnetic resonance (1H NMR), and gel permeation chromatography (GPC) measurements. Subsequently, the obtained triblock copolymers could self-assemble into micelles in aqueous solution owing to their amphiphilic property resulting from the hydrophobic β-CD core and the hydrophilic PHEMA, PNIPAM, and PDMAEMA segments, which were investigated by dynamic light scattering (DLS) and transmission electron microscopy (TEM). The triblock copolymer nanoparticles exhibited two separate lower critical solution temperatures (LCSTs) and these triblock copolymers in aqueous solutions were investigated at the different pH values of 5.0 and 7.4 as doxorubicin (DOX) delivery systems for controlled release. Polymeric micelles exhibited a pH controlled release performance. Investigation of DOX release from the micelles indicated that the release rate of the drug could be effectively controlled by altering the pH, encapsulating DOX in neutral surroundings (blood circulation or extracellular matrix) and triggering DOX release in acidic surroundings (intracellular endosomes). CCK-8 assays and confocal laser scanning microscopy (CLSM) against HeLa cells indicated that the polymeric micelles themselves had no associated cytotoxicity and possessed good biodegradability and biocompatibility and that the micelles identified the location of the DOX in the HeLa cells. On the other hand, the DOX-loaded micelles possessed a high cytotoxicity to HeLa cells and exhibited inhibition of the proliferation of HeLa cells. Hence, this type of biodegradable, biocompatible, and stimuli-responsive copolymer could serve as a promising material for drug delivery.
研究领域:
高分子化学
·
材料科学
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药学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2020
·
IF 0
Ke Ma,Xinran Wei,Jiao Liu,Daijun Chen,Xiaoli Zhao,Jiwei Shen,Hao Lu,Pengxiang Jia
A number of well-controlled amphiphilic copolymers of poly(2-methacryloyloxyethyl phosphorylcholine-co-azobenzene methacrylamide) (PMA), carrying both cell outer membrane mimetic phosphorylcholine zwitFVterion groups and ultraviolet (UV)-sensitive hydrophobic azobenzene units, were prepared by reversible addition–fragmentation chain-transfer polymerization and applied to fabricate a novel UV/near-infrared (NIR) dual-responsive cell membrane mimetic micelle through the encapsulation of upconversion nanoparticles (UCNPs) into PMA micelles. The diameter of the UCNPs@PMA micelles could be well-controlled by adjusting the feeding ratio between UCNPs and PMA. The obtained UCNPs@PMA micelles displayed a controllable drug-release performance by tuning the duration of UV/NIR-light irradiation. Furthermore, the UCNPs@PMA micelles showed enhanced biocompatibility and a remarkable NIR-triggered therapeutic effect. Interestingly, doxorubicin-loaded UCNPs@PMA micelles showed pH-responsive drug release and much higher cytotoxicity of the tumor cell than of the normal cell after NIR laser irradiation. Therefore, the UCNPs@PMA micelles may serve as a powerful on-demand chemotherapy platform in cancer therapy via the NIR-light-triggered drug-release ability.
研究领域:
材料科学
·
纳米技术
·
药学
·
生物医学工程
·
癌症研究
✓ 已验证
产品关联
DOI
危险品化学品经营许可证（带存储）