INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY
·
2019
·
IF 2.18
Wei Chen,Jing Zhang,Chen Li,Quan Pan,Jingtong Wu,Lina Fan,Chunyan Chen,Xiaoqing Huang,Fei Teng,Jinshui Zhu
Animal models play critical roles in exploring the pathogenesis of human diseases and designing novel therapeutic schemes. Acute experimental colitis (AEC) models have been reported to be established in mice principally by oral administration of dextran sulfate sodium (DSS). However, little knowledge is known about whether DSS can be used to induce the acute experimental enteritis (AEE). In this study, different concentrations of DSS (0%, 2%, 3%, and 5%) were used to induce AEC and AEE models in two cohorts. After the establishment of these two models, the symptoms of the mice induced by DSS were noted, the length and average weight of each colon and small intestine were measured, and hematoxylin and eosin (HE) staining was conducted for assessing the inflammatory infiltration in these models. Generally, the comparison of the inflammatory scoring between AEC and AEE models was analyzed. As a consequence, we found that, the mice with 2%–5% DSS administration in a week could develop into AEC models in two cohorts and AEE models in one cohort, followed by the signs of diarrhea, gross rectal bleeding, weight loss of the body, and shortened colon and intestine length, as compared with the control group. HE staining showed that the inflammatory scoring was dramatically increased by 3%–5% DSS in AEC models in two cohorts but slightly elevated in AEE models in one cohort. Meanwhile, as compared with the severe AEC models, the extent of inflammatory infiltration induced by 3%–5% DSS in AEE models was much milder. In conclusion, oral administration of 3%–5% DSS is a good inducer of AEC models, but not AEE models.
研究领域:
免疫学
·
胃肠病学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2014
·Vol. 4
·期 95
·52940-52948
·
IF 3.708
Shasha Wang,Fang Yuan,Gaojian Chen,Kehua Tu,Hongjun Wang,Li-Qun Wang
The potential toxicity towards human kidneys of hemoglobin (Hb), when used directly, has severely limited its application as a red blood cell substitute and in cancer treatments. In this work, a novel hemoglobin–polymer conjugate was prepared by a reaction between the lysine amino groups of Hb and the carboxyl groups of a copolymer, poly(N-isopropylacrylamide) grafted carboxylated dextran (HOOC-Dex-g-PNIPAAm), which was synthesized by single electron transfer living radical polymerization (SET-LRP) and post-carboxylation. The conjugate was characterized by FTIR, 1H NMR, SDS-PAGE, DLS, fluorescence spectroscopy and TEM. Results showed it had a relatively low critical micelle concentration (CMC) and could form stable spherical nanoparticles upon heating above the LCST. The redox activity and gas-binding capacity of the Hb conjugate were subsequently confirmed by UV-vis spectroscopy, indicating the retention of Hb bioactivity after conjugation. Furthermore, dextran with different numbers of PNIPAAm chains was synthesized for comparison. It revealed that at 37 °C, the temperature above the LCST, the conjugation of Hb to the copolymer Dex-g-PNIPAAm could improve the stability of Hb which increased with the number of PNIPAAm chains.
研究领域:
生物材料学
·
医学工程
·
血液替代品研究
✓ 已验证
DOI
高影响因子
METABOLIC BRAIN DISEASE
·
2025
·Vol. 40
·期 5
·1-14
·
IF 3.5
Zhao Hexiang,Yang Ping,Zhang Mou,Zheng Wenshu,Qi Renli,Zhu Xiaofeng,Li Jinghui,Li Shipeng
Reperfusion, while essential for restoring blood supply, paradoxically exacerbates neuronal damage through cerebral ischemia-reperfusion injury (CIRI). This study aimed to develop a reactive oxygen species (ROS)-responsive drug delivery system (DDS) loaded with edaravone (EDA) to enhance targeted therapy for CIRI. The stimuli-responsive DDS was synthesized using dextran (DEX) as the biocompatible carrier and benzeneboronic acid pinacol ester (BAPE) as the ROS-sensitive moiety. The physicochemical characteristics of the DEX-BAPE/EDA (DB/EDA) micelles were systematically evaluated. In vitro studies assessed the anti-inflammatory, antioxidant, and anti-apoptotic effects of DB/EDA. Moreover, the neuroprotective efficacy of DB/EDA in vivo was analyzed via behavioral tests, infarct volume measurement, ELISA assays of inflammatory cytokines and OS markers, and Western blot analysis of Nrf2-related pathways. Pharmacokinetics and biosafety were analyzed through plasma profiling and H&E staining. DB/EDA exhibited high stability, efficient drug encapsulation, and ROS-responsive drug release. Cellular uptake studies confirmed enhanced internalization of DB/EDA micelles in BV2 cells. In the oxygen-glucose deprivation/reoxygenation (OGD/R) model, DB/EDA significantly suppressed TNF-α, IL-1β, IL-6, and MDA, restored SOD levels, and attenuated apoptosis. In the middle cerebral artery occlusion/reperfusion (MCAO/R) mice, DB/EDA administration effectively improves cognition and mitigates neuronal damage. Mechanistically, DB/EDA activated the Nrf2/HO-1 pathway, amplifying antioxidant and anti-inflammatory responses. Pharmacokinetic analysis revealed prolonged circulation and increased brain accumulation, and histopathological analysis demonstrated the safety profile of DB/EDA. The ROS-responsive DB/EDA nano-micelles provided targeted EDA delivery to ischemic brain regions, alleviating CIRI via Nrf2 activation, suggesting that DB/EDA is a promising strategy for CIRI treatment. Graphical abstract
研究领域:
药剂学
·
神经科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Jia Liu,Chao Qi,Kaixiong Tao,Jinxiang Zhang,Jian Zhang,Luming Xu,Xulin Jiang,Yunti Zhang,Lei Huang,Qilin Li,Hongjian Xie,Jinbo Gao,Xiaoming Shuai,Guobin Wang,Zheng Wang,Lin Wang
Severe side effects of cancer chemotherapy prompt developing better drug delivery systems. Injectable hydrogels are an effective site-target system. For most of injectable hydrogels, once delivered in vivo, some properties including drug release and degradation, which are critical to chemotherapeutic effects and safety, are challenging to monitor. Developing a drug delivery system for effective cancer therapy with in vivo real-time noninvasive trackability is highly desired. Although fluorescence dyes are used for imaging hydrogels, the cytotoxicity limits their applications. By using sericin, a natural photoluminescent protein from silk, we successfully synthesized a hydrazone cross-linked sericin/dextran injectable hydrogel. This hydrogel is biodegradable and biocompatible. It achieves efficient drug loading and controlled release of both macromolecular and small molecular drugs. Notably, sericin’s photoluminescence from this hydrogel is directly and stably correlated with its degradation, enabling long-term in vivo imaging and real-time monitoring of the remaining drug. The hydrogel loaded with Doxorubicin significantly suppresses tumor growth. Together, the work demonstrates the efficacy of this drug delivery system, and the in vivo effectiveness of this sericin-based optical monitoring strategy, providing a potential approach for improving hydrogel design toward optimal efficiency and safety of chemotherapies, which may be widely applicable to other drug delivery systems.
研究领域:
生物材料学
·
肿瘤学
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Biomedical Nanotechnology
·
2021
·Vol. 17
·期 6
·1208-1216
·
IF 4.099
Yang Shuqin,Sun Xiaoyan,Wang Yanmei,Bie Xiaoyan,Fan Tianren
Lidocaine-loaded nanoparticles are versatile nanomaterials that may be used in pain treatment due to their wound healing properties. The current study describes a wound dressing formulation focused on lidocaine-loaded dextran/ethylene glycol nanoparticles (an anesthetic drug). The lidocaine-loaded
dextran/ethylene glycol membranes were fabricated using lidocaine solutions inside the dextran/ethylene glycol medium. The influence of various experimental conditions on dextran/ethylene glycol nanoparticle formations were examined. The sizes of dextran/ethylene glycol and lidocaine-loaded
dextran/glycol nanoparticles were examined through the HR-SEM. Moreover, the efficacy antibacterial activity of dextran/glycol and lidocaine-loaded dextran/ethylene glycol nanoparticles was evaluated against the microorganisms grampositive and negative. Furthermore, we observed the In Vivo
wound healing of wounds in skin using a mice model over a 16 days period. In this difference to the wounds of untreated mouse, quick healing was observed in the lidocaine-loaded dextran/glycol nanoparticles-treated wounds with fewer injury. These results specify that lidocaine-loaded dextran/ethylene
glycol nanoparticles-based dressing material could be a ground-breaking nanomaterial having wound repair and implantations potential required for wound injury in pain management, which was proven using an animal model.
研究领域:
药学
·
纳米技术
·
生物医学工程
·
药理学
·
伤口护理
✓ 已验证
DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2024
·
IF 5.8
Chenyu Liu,Shuli Yue,Ruizhi Li,Lin Wang,Kun Zhang,Shiwei Wang,Simiao Yu,Farishta Shafiq,Yao Liu,Weihong Qiao
In response to the pain, swelling, and redness resulting from inadequate wound hemostasis and anti-inflammatory measures, there is a pressing need to develop wound repair materials capable of promptly stopping bleeding, facilitating painless dressing changes, and expediting wound closure to enhance skin wound recovery. However, comprehensive research is deficient concerning the conformational relationship of hyaluronic acid (HA) with various molecular weights concerning the structure of hydrogel materials. This study aims to address this need by utilizing aminated hyaluronic acid (AHA) and aldehyde-modified dextran (DA) to prepare dextran-based hyaluronic acid hydrogels (DA-AHA) via Schiff base reaction, imbuing them with acute inflammation repair and wound healing promotion properties. In vitro, coagulation experiments demonstrated that the DA-AHA hydrogel could rapidly coagulate blood within 60 s. Subsequent animal experiments corroborated these findings, revealing the hydrogel’s efficacy in significantly shortening wound healing time and accelerating wound closure efficiency. Moreover, MTT and live-death experiments affirmed the non-cytotoxic nature of the synthesized hydrogel. The study delved into the structural regularity and wound-healing efficacy of DA-AHA hydrogel materials featuring various molecular weights. Additionally, it compared the hemostatic and wound-healing properties of (high molecular weight dextran-aminated hyaluronic acid hydrogel) HDA-AHA, demonstrating its superiority in both aspects.
研究领域:
材料科学
·
生物材料
·
伤口愈合
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2024
·
IF 3.2
Fengjiao Fan,Chenlinrui Yue,Zhenni Zhai,Hailu Liao,Xiaoni Lian,Hongkai Xie
Scallops are rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid but perishable due to their microbial growth and lipid oxidation. In this study, gelatin/dextran films containing cinnamaldehyde and α-tocopherol (0% + 0%, 0.3% + 0.3%, 0.6% + 0.6%, 0.9% + 0.9%, and 1.2% + 1.2%, w/w) as active fillers were developed by solution casting method, and their preservation effects on scallop adductor muscle refrigerated at 4°C for 0, 3, 6, 9, and 12 days were evaluated. Inclusion of the two active fillers did not influence the thermal stability of the films but created heterogenous and discontinuous film microstructure and increased the film hydrophobicity. Increase in the concentrations of active fillers lowered the mechanical properties and water vapor permeability of the films but increased their crystallinity, thickness, water contact angle, opacity, antibacterial property, and antioxidant property. The longest release times for both cinnamaldehyde and α-tocopherol were found in 95% (v/v) ethanol solution. The gelatin/dextran films containing 1.2% (w/w) of active fillers (Gelatin [Ge]/Dextran [Dx]/1.2 film) improved the chemical stability of refrigerated scallop adductor muscle. The total viable count (TVC) of the unpackaged scallop adductor muscle exceeded the recommended limit of 7 lg CFU/g on day 6 (7.07 ± 0.50 lg CFU/g), whereas the TVC of the Ge/Dx/1.2 film-packaged scallop adductor muscle was still below the limit on day 9 (5.60 ± 0.50 lg CFU/g). Thus, the Ge/Dx/1.2 film can extend the shelf life of refrigerated scallop adductor muscle by at least 3 days. Overall, the developed gelatin/dextran active packaging films are promising for the preservation of aquatic food products.
研究领域:
食品科学
·
材料科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
·
2024
·Vol. 113
·期 1
·e37847
·
IF 3.9
Jianing Cheng,Liguo Hao,Xiaorong Zhu,Ruifan Ma,Silong Li,Qiangqiang Yin,Dongxu Wang,Haifeng Hu,Tianyu Zhang,Zheng Li,Houyi Cong,Xiaoyang Zheng,Jun He,Yuguang Wang
Triggered by the vulnerability to atherosclerotic plaques, cardiovascular diseases (CVDs) have become a main reason for high mortality worldwide. Thus, there is an urgent need to develop functional molecular imaging modalities to improve the detection rate of vulnerable plaques. In this study, polyethyleneimine (PEI) was coated on the surface of mesoporous silica nanoprobes (MSN) loaded with Gd 2 O 3 (MSN@Gd 2 O 3 ), followed by coupling the fluorescent dye carboxylated heptamethine cyanine (IR808), and then the dextran sulfate (DS) was modified on the surface of MSN@Gd 2 O 3 @IR808 by electrostatic adsorption, to construct a targeted and pH-responsive magnetic resonance (MR)/near-infrared fluorescence imaging (NIRF) dual-modal nanoprobe (MSN@Gd 2 O 3 @IR808@DS nanoparticles). The nanoprobe presented a more concentrated distribution of spherical shapes in transmission electron microscopy. In vitro simulated vulnerable plaque microenvironment (pH = 5.5) presented significant T 1 -weighted imaging (T 1 WI) signal and longitudinal relaxation in the nanoprobe. Immunofluorescence staining and cellular uptake assays showed that MSN@Gd 2 O 3 @IR808@DS nanoparticles have the ability to specially bind to scavenger receptors A (SR-A). In vascular endothelium from the high-fat diet (HFD) New Zealand White rabbits, MSN@Gd 2 O 3 @IR808@DS nanoparticles can exhibit specific contrast-enhanced signals by MR/NIRF dual-modal imaging. In addition, cytotoxicity assays and hematoxylin and eosin (H&E) staining results demonstrated that MSN@Gd 2 O 3 @IR808@DS nanoparticles have good biocompatibility. Hence, this multifunctional MR/NIRF bimodal nanoprobe provides new experimental and technological ideas for the accurate diagnosis of vulnerable plaques.
研究领域:
医学影像学
·
生物材料学
·
心血管病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2020
·
IF 3.862
Tarig Elshaarani,Haojie Yu,Li Wang,Long Lin,Nan Wang,Kaleem ur Rahman Naveed,Li Zhang,Yin Han,Shah Fahad,Zhipeng Ni
Different glucose-responsive nanogels of N -isopropylacrylamide (NIPAM) and 4-(1,6-dioxo-2,5-diaza-7-oxamyl) phenylboronic acid (DDOPBA) were synthesized using dextran-grafted maleic acid (Dex-MA) as a crosslinker. The formed nanogels (P(NIPAM-co-Dex-co-DDOPBA)s) were verified by 1 H NMR, TEM, DLS and X-ray photoelectron spectroscopy (XPS). The incorporation of DDOPBA provided a remarkable sensitivity towards glucose in physiological pH due to the existence of electron-withdrawing group in its structure. Similarly, the hydrophilic Dex-MA modulated the temperature-sensitivity near physiological temperature. The nanogels exhibited high insulin loading capacity and encapsulation efficiency and the in vitro release profiles demonstrated a glucose dependant release of the payload at physiological pH and temperature.
研究领域:
生物材料学
·
药物递送系统
✓ 已验证
产品关联
DOI
高影响因子
Regenerative Biomaterials
·
2022
·
IF 5.763
Jiang Linrui,Zheng Rong,Zeng Ni,Wu Changqiang,Su Hongying
Polymeric micelles have long been considered as promising nanocarrier for hydrophobic drugs and imaging probes, due to their nanoscale particle size, biocompatibility and ability to loading reasonable amount of cargoes. Herein, a facile method for dextran micelles preparation was developed and their performance as carriers of superparamagnetic iron oxide (SPIO) nanocrystals was evaluated. Amphiphilic dextran (Dex-g-OA) was synthesized via the Schiff base reactions between oxidized dextran and oleylamine, and self-assembled in situ into nano-size micelles in the reaction systems. The self-assembling behaviors of the amphiphilic dextran were identified using fluorescence resonance energy transfer technique by detection the energy transfer signal between the fluorophore pairs, Cy5 and Cy5.5. Hydrophobic SPIO nanoparticles (Fe3O4 NPs) were successfully loaded into the dextran micelles via the in situ self-assembly process, leading to a series of Fe3O4 NPs-loaded micelle nanocomposites (Fe3O4@Dex-g-OA) with good biocompatibility, superparamagnetism and strongly enhanced T2 relaxivity. At the magnetic field of 0.5 T, the Fe3O4@Dex-g-OA nanocomposite with particle size of 116.2 ± 53.7 nm presented a higher T2 relaxivity of 327.9 mM−1Fe·s−1. The prepared magnetic nanocomposites hold the promise to be used as contrast agents in magnetic resonance imaging.
研究领域:
材料科学
·
纳米技术
·
生物医学工程
·
分子影像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2016
·
IF 3.138
Hongyu Chen,Diwei Zheng,Jia Liu,Ying Kuang,Qilin Li,Min Zhang,Haifeng Ye,Hongyang Qin,Yanglin Xu,Cao Li,Bingbing Jiang
In this work, a novel pH-sensitive drug delivery system based on modified dextrin coated mesoporous silica nanoparticles (MSNs), DOX@MSN-DDA-CL, are prepared. The dextrin grafting on the surface of MSNs is oxidized by KIO 4 to obtain dextrin dialdehyde, which is then cross-linked by tetraethylenepentamine through a pH-sensitive Schiff’s base. Under physiological conditions, the cross-linked dextrin dialdehyde blocks the pores to prevent premature release of model drug doxorubicin hydrochloride (DOX). In the weak acidic environment, pH 6.0 in this work, the Schiff’s base can be hydrolyzed and released the drug. The in vitro drug release studies at different pHs prove the pH-sensitivity of DOX@MSN-DDA-CL. The cytotoxicity and cell internalization behavior are also investigated in detail. In vivo tissue distribution and pharmacokinetics with a H22-bearing mouse animal mode are also studied, prove that DOX@MSN-DDA-CL has a longer retention time than that of pure DOX and can accumulate in tumor region via enhanced permeation and retention and nanomaterials-induced endothelial cell leakiness effects. In conclusion, the pH-sensitive modified dextrin/MSNs complex drug delivery system has a great potential for cancer therapy.
研究领域:
纳米技术
·
药剂学
·
癌症治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMER BULLETIN
·
2025
·Vol. 83
·期 1
·31
·
IF 4
Huang Ruoxi,Yi Yaxin,Zhang Shiyu,Wang Jiaxin,Yuan Haikuan,Lu Jie
A stimulus-responsive drug delivery system holds promise for reducing adverse effects and enhancing the bioavailability of chemotherapeutic agents. In this study, amino-terminated dextran was utilized as a macromolecular initiator to prepare a range of amphiphilic dextran-b-poly(ε-benzyloxycarbonyl- l -lysine) (Dex-b-PZLL) copolymers with varying hydrophobic segment lengths via ring-opening polymerization (ROP). Folic acid (FA) was further grafted onto the dextran side chains via esterification (FA-Dex-b-PZLL), offering the potential tumor-specific recognition. The resulting copolymers were able to self-assemble into stable spherical micelles via dialysis and efficiently encapsulated the hydrophobic drug letrozole (LTZ). A systematic assessment was conducted on the drug encapsulation efficiency/loading capacity, drug release performance in vitro, and biocompatibility of the copolymers under aqueous conditions, indicating that these properties of the FA-Dex-b-PZLL micelles mainly depended on the copolymer composition. The optimized micelles, with a balanced hydrophilic/hydrophobic ratio, achieved a drug loading capacity with approximately 8.49% and an encapsulation efficiency with about 46.39%. Moreover, LTZ/FA-Dex-b-PZLL micelles exhibited pH-responsive release, with cumulative release rates within 72 h of 20.74%, 27.14% and 62.96% in phosphate buffer at pH 7.4, 6.5 and 4.5, respectively, indicating the accelerated release under acidic conditions. In addition, the micelles showed good cytocompatibility according to the CCK-8 assay. Graphical abstract
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）