FOOD CHEMISTRY
·
2022
·
IF 9.231
Congnan Cen,Jian Chen,Weiqiang Wang,Jie Zhang,Xiangying Yang,Linglin Fu,Yanbo Wang
This study aims to reveal the mechanism of the interaction between folic acid (FA) and egg ovalbumin (OVA) through the method of multi-spectroscopic, molecular docking, and molecular dynamics simulation in order to probe OVA as the possibility of a carrier of unstable vitamins. The results of the fluorescence spectra indicated a static quenching in the OVA-FA with a strong affinity of 6.998 × 10 4 M −1 . At the same time, the complex formed by FA and OVA has changed the microenvironment. The measurement results of circular dichroism and particle size showed that FA and OVA gradually formed larger particles without changed the secondary structure of the protein. In addition, the results of molecular simulations indicated that the interaction between OVA and FA is mainly stabilized by strong hydrophobic and hydrogen bonds. This research was expanded the application prospect of dietary protein OVA as a transportation and protection system of vitamin substances.
研究领域:
食品科学
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生物化学
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分子生物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Fengjie Liu,Fansu Meng,Xiaoshan Hong,Anil K. Giri,Mulazim Hussain Asim,Zhong Chen,Yiqi Chen,Yixuan Lin,Lulu He,Qiaowen Bu,Yong Huang,Xiaoli Sun,Zhenjiang Yang,Yu Cai
Background Baicalin (BA), a natural flavonoid compound, exhibits potential anti-breast cancer (BC) activity. However, its therapeutic efficacy is limited by biological membrane and tumor barriers. Albumin nanoparticles demonstrate excellent biocompatibility and ease of modification, making them highly advantageous for antitumor drug delivery. Despite these advantages, current preparation methods for albumin-based NPs have significant limitations, including the use of organic or toxic reagents, large and uneven particle size products, complex processes, etc. Objective The purpose of this study was to develop a novel method for preparing albumin nanoparticles (BSA NPs). Subsequently, BSA NPs were modified with folic acid (FA) and polymers and loaded with BA to construct a novel nanodrug delivery system (BA@mPEG-FA-BSA NPs) to improve the anti-breast cancer efficacy of BA. Methods Compared with the desolvation method and the thermal gelation method, the advantages of the new preparation method of BSA NPs are: less organic solvent and preparation time, greater yield, smaller particle size and uniform product. The preparation method of BSA NPs was investigated by single factor experiment. The encapsulation efficiency (EE) and drug loading (DL) of BA in BA@mPEG-FA-BSA NPs were detected by HPLC. The structural composition and physicochemical properties of the nanoparticles were characterized using transmission electron microscopy (TEM), infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), and X-ray diffraction (XRD). The anti-breast cancer efficacy of BA@mPEG-FA-BSA NPs was evaluated through cellular uptake, cytotoxicity, apoptosis, cell cycle analysis, and in vivo tumor-bearing mouse studies. Results According to the setting of process parameters, the established method can control the acquisition of uniform BSA NPs with a particle size between 20 and 300 nm. BA@mPEG-FA-BSA NPs exhibited a particle size of approximately 59.26 nm, a polydispersity index (PDI) < 0.3, EE of about 46.7 %, and DL of approximately 2.6 %. Cellular uptake experiments demonstrated that mPEG-FA-BSA NPs enhanced the uptake of free coumarin 6 in BT20 cells, with free FA showing competitive inhibition effects. Efficacy evaluation revealed that BA@mPEG-FA-BSA NPs exhibited cytotoxicity against BT20 cells, induced cell cycle arrest and apoptosis, and inhibited tumor growth in 4 T1 tumor-bearing mice. In most cases, the effects of BA@mPEG-FA-BSA NPs were superior to those of free BA, with a concentration- or dose-dependent manner observed. Conclusion This study established a novel preparation method for albumin nanoparticles, providing a new methodology for the construction of nano-drug delivery systems based on albumin materials. The developed BA@mPEG-FA-BSA NPs, with their relatively small particle size, demonstrated enhanced cellular uptake and anti-breast cancer activity of BA, highlight the potential for further optimization and clinical translation.
研究领域:
药学
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肿瘤学
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纳米医学
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DOI
PubMed
高影响因子
ACS Biomaterials Science & Engineering
·
2020
·
IF 4.152
Le Gao,Zhitao Wu,Abdul-Rauf Ibrahim,Shu-Feng Zhou,Guowu Zhan
Hollow nanomaterials have been used as an attractive platform for the integration of multiple bioactive components for effective anticancer therapy. Herein, we report a novel and facile strategy for the fabrication of hollow and monodispersed zeolitic imidazolate framework-8 (ZIF-8) by the self-template method with folic acid (FA) as a bioetcher. Gold nanocluster and folic acid were critical for the formation of the hollow ZIF-8 (thickness of 38 nm) during solvothermal synthesis. By integrating CuS nanoparticles (size of 4.9 nm), the resultant quadruple ZIF-8/Au/CuS/FA nanocomposites (denoted as FACZ) exhibited effective anticancer activities on FA receptor-positive MCF-7 and HepG-2 tumor cells but a weak killing effect on HCMEC/D3 cells. Folic acid molecules were conjugated to the external surface of FACZ, which simultaneously offered an excellent tumor-targeting ability and fluorescence imaging property. Although the photothermal therapy caused by CuS was not so obvious due to partial reduction, the nanosized FACZ after cellular uptake was able to release Cu(I) to enable chemodynamic therapy. This catalytically decomposed H2O2 to produce highly reactive oxygen species via the Fenton-like reaction as determined by the extracellular and intracellular hydroxyl radical. Our work offers a simple route for the fabrication of hollow ZIF-8 nanocomposite with active and selective anticancer activity. This is envisaged to have great potentials in biomedical applications.
研究领域:
生物医学工程
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肿瘤学
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纳米医学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS APPLICATIONS
·
2019
·
IF 2.442
Yu Gao,Tao Liu,Xuan Liu,Chao Wu
Folic acid functionalized gelatin-coated mesoporous hollow carbon nanospheres (FGMCN) were synthesized and applied to enhance the antitumor curative effect of paclitaxel (PTX) for human liver cancer cell lines (SMMC-7721). PTX was loaded in FGMCN by the adsorption method and the PTX-loaded samples (PTX-FGMCN) had a drug content of 29.8 ± 1.06%. The PTX-FGMCN with a sustained release effect was characterized by X-ray diffraction and differential scanning calorimeter in order to analyze the PTX state in FGMCN. In vitro cell experiments showed that FMHSN improves the uptake of PTX and promotes apoptosis due to the nano-targeting effect of FMHSN. An in vivo tumor bearing experiment in mice indicated that the PTX-FGMCN significantly inhibited the growth of tumors. All of these results suggested that the PTX-FGMCN may be an effective anti-hepatoma drug in the future.
研究领域:
药学
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肿瘤学
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纳米医学
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产品关联
DOI
PubMed
高影响因子
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
·
2021
·Vol. 53
·期 8
·1027-1036
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IF 3.848
Yao Wang,Yao Jialiang,Qian Fangfang,Que Zujun,Yu Pan,Luo Tianle,Zheng Dan,Zhang Zhanxia,Tian Jianhui
Targeted delivery and smart response of nanomedicine hold great promise for improving the therapeutic efficacy and alleviating the side effects of chemotherapy agents in cancer treatment. However, availability of only a few studies that discuss organic nanomedicines with these properties limits the development prospects of nanomedicines. In the present study, folic acid (FA)-targeted delivery and glutathione (GSH) smart responsive nanomedicine were rationally designed for paclitaxel (PTX) delivery for the treatment of lung cancer. Compared with other stimuli-responsive nanomedicines, this nanocarrier was not only sensitive to biologically relevant GSH for on-demand drug release but also biodegradable into biocompatible products after fulfilling its delivery task. The nanomedicine first entered tumor cells via FA and its receptor-mediated endocytosis. After the lysosomal escape, poly(lactic-co-glycolic acid) (PLGA) nanomedicine was triggered by a higher level of GSH and released its cargo into the tumor microenvironment. In vitro and in vivo results revealed that the PLGA nanomedicine not only inhibited the proliferation and promoted the apoptosis of lung cancer cells significantly but also possessed less toxic side effects when compared with free PTX. Therefore, the proposed drug delivery system demonstrates the potential of a multifunctional nano-platform to enhance bioavailability and reduce the side effects of chemotherapy agents.
研究领域:
药剂学
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肿瘤学
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纳米医学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Xin Hai,Yiting Wang,Xiaoyao Hao,Xuwei Chen,Jianhua Wang
Folic acid (FA) encapsulated graphene quantum dots (FA-GQDs) were prepared via a one-pot microwaved alkali cutting method, using graphene oxide (GO) and FA as raw material. TEM and AFM images revealed that FA was successfully cross-linked on the surface of GQDs under alkali circumstance, giving rising to stable and homogeneous FA-GQDs with size of about 33.6 nm. XPS and FT-IR confirmed that there are abundant hydroxyl, carboxyl and amino groups on the encapsulated FA-GQDs. The as-prepared FA-GQDs possessed the fluorescence behaviors of multi-excitation and emission sites, and exhibited sensitive response to the pH variation within the pH range of 3–9, making it excellent fluorescence probe for pH sensing in cell lysate and environmental water samples. The broad emission with pH-sensitive characteristic and low toxicity of FA-GQDs also provided an alternative for the specific multicolor imaging and sensing of pH in living cells.
研究领域:
生物化学
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纳米医学
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分析化学
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产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2017
·Vol. 409
·期 30
·7063-7075
·
IF 3.431
Wang Meng,Jiao Yang,Cheng Chunsheng,Hua Jianhao,Yang Yaling
A novel and sensitive method based on nitrogen-doped carbon quantum dots as a fluorescence probe coupled with magnetic solid-phase extraction (MSPE) purification for analysis of folic acid (FA) in human serum samples has been established for the first time. In the developed system, magnetic nanoparticles coated with hexanoic acid (Fe 3 O 4 @C 6 ) were synthesized by a one-step chemical co-precipitation method with good magnetic properties and dispersibility for sample purification, and it is better to be separated from the sample. High fluorescence nitrogen-doped carbon quantum dots (N-CQDs), simply prepared using a one-step hydrothermal method with nitrilotriacetic acid, could be selectively quenched by FA. Based on this phenomenon, a fluorescence assay was proposed for specific determination of FA. Various operational experiment parameters have been studied and optimized in detail. Under the optimum experimental conditions, the detection limit of the proposed method for FA was evaluated to be 0.5 nM (S/ N = 3), while the relative standard deviation (RSD) was 1.2% ( n = 6). Finally, the proposed method was applied for determination of trace levels of FA from human serum samples and quantitative recoveries were achieved within the range of 95.7–103.5%. All of the results showed that the proposed method had significant application in further research.
Schematic of synthesis of N-CQDs and schematic of suggested mode for analysis of folic acid (FA).
研究领域:
分析化学
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生物医学
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产品关联
DOI
PubMed
高影响因子
DRUG DELIVERY
·
2019
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IF 3.829
Mengna Liu,Bingjie Wang,Chunjing Guo,Xiaoya Hou,Ziting Cheng,Daquan Chen
In this study, novel multifunctional folic acid, biotin, and CD44 receptors targeted and pH-sensitive “nano-actiniaes” were fabricated with icariin (ICA) and curcumin (Cur) as loaded model drugs for breast cancer therapy. The newly synthesized polymer oligomeric hyaluronic acid-hydrazone bond-folic acid-biotin (Bio-oHA-Hyd-FA) was characterized by 1H NMR spectrogram (proton nuclear magnetic resonance). The obtained drug carrier Bio-oHA-Hyd-FA self-assembled into nanomicelles, named as “nano-actiniaes”, in aqueous media with hydrodynamic diameter of 162.7 ± 5 nm. The size, surface zeta potential, and morphology of the “nano-actiniaes” were observed via TEM. The in vitro release experiment indicated that much more encapsulated icariin (ICA) and curcumin (Cur) were released from the Bio-oHA-Hyd-FA micelles (nano-actiniaes) in the acidic environment. Additionally, the cytotoxicity research demonstrated that the Bio-oHA-Hyd-FA carrier material was completely nontoxic, and the ICA&Cur “nano-actiniaes” had greater cytotoxicity compared with other control groups. In addition, the “nano-actiniaes” were found to significantly inhibit cancer cell invasion by Transwell assay. Moreover, in vivo evaluation of anti-tumor effect illustrated that the ICA and Cur “nano-actiniaes” possessed inhibitory effect on tumors. Consequently, the multi-targeted pH-sensitive “nano-actiniaes” can realize significant tumor targeting and effectively inhibit tumor growth.
研究领域:
药剂学
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肿瘤学
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生物材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF LUMINESCENCE
·
2019
·
IF 2.961
Si Yao,Li Chen,Fang Jia,Xinzhi Sun,Huifang Su,Hongjian Liu,Lixia Yang,Zejiang Wang,Fengshou Wu,Kai Wang
For a precisely targeted photosensitizer , we designed and prepared a new platinated porphyrin ( Pt-Por-FA ) by Pt-Por tailed with folic acid ( FA ). The intercalation mode of Pt-Por-FA binding to ct-DNA was confirmed by UV–vis and fluorescent spectra. The singlet oxygen generation of Pt-Por-FA was evaluated through 2′, 7′-dichlorofluorescin (DCFH) method. The cellular uptake and the intracellular localization of Pt-Por-FA were studied upon A549 and HeLa cells through confocal laser scanning microscope. The results showed that the red fluorescence of porphyrin conjugate observed in HeLa cells [folate receptor (FR)–positive] was significantly stronger than that of A549 cells (FR-negative), indicating that Pt-Por-FA could target specifically the cancer cells with overexpression of FR via FR-mediated endocytosis. Pt-Por-FA successfully localized in the lysosomes of cancer cells, but Pt-Por without FA did not. Moreover, the cytotoxicity against HeLa and MDA-MB-231 cells was measured by MTT assay . The results demonstrated that Pt-Por-FA had minor cytotoxicity in the dark, but showed a obviously improving toxicity in presence of light. At the concentration of 20 μM Pt-Por-FA , the cell viabilities were only 12.0% and 26.4% with the IC 50 values down to 5 μm and 12 μm against HeLa and MDA-MB-231 cells, respectively. It suggests that Pt-Por-FA could be a precisely targeted photosensitizer with over-expression of a folate receptor.
研究领域:
药理学
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肿瘤学
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生物化学
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产品关联
DOI
高影响因子
Journal of Nanomaterials
·
2017
·
IF 1.871
Juan Zhang,Huanhuan Weng,Xiangwan Miao,Quanming Li,Siqi Wang,Huifen Xie,Tao Liu,Minqiang Xie
A novel folate (FA) receptor-targeted superparamagnetic Fe3O4 nanoparticles (SPIONs) codelivering cisplatin (CDDP) and tissue factor pathway inhibitor-2 (TFPI-2) plasmid DNA (pDNA) was constructed. The core shell nanocomposites ([email protected]) were composed of superparamagnetic Fe3O4 core that binds CDDP and TFPI-2 shell that combines with folate-polyethylene glycol-polyethyleneimine (FA-PEG-PEI) via electrostatic interaction. The shell containing FA-PEG-PEI and TFPI-2 plasmid was synthesized through amidation reaction and electrostatic adsorption and the core containing SPION-CDDP was modified by aldehyde sodium alginate. Proton nuclear magnetic resonance and Fourier transform infrared spectra on FA-PEG-PEI polymers showed characteristic peaks of various metabolites in corresponding frequency. Transmission electron microscopy image of [email protected] nanoparticles demonstrated a near-monodisperse spherical morphology, while dynamic light scattering studies indicated an intensity-average diameter of 149.5 nm. Zeta potential was 14.89 ± 1.83 mv and the final concentration of loaded CDDP was 100 ug/ml. Gel electrophoresis data showed that the nanocomposite would protect TFPI-2 pDNA from being digested by DNases. Compared with CNE-2 cells, the good targetability and better gene transfection efficiency (57.9%) were detected by Prussian blue iron stain and fluorescence analysis in HNE-1 cells in vitro. The results suggested the potential application of [email protected] as a multifunctional anticancer nanomedicine on targeting therapy for FR positive NPC.
研究领域:
纳米技术
·
癌症治疗
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药学
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生物医学工程
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产品关联
DOI
高影响因子
JOURNAL OF INORGANIC BIOCHEMISTRY
·
2018
·
IF 3.063
Min Wang,Xiaofang Fei,Shaowu Lv,Ye Sheng,Haifeng Zou,Yanhua Song,Fei Yan,Qianlong Zhu,Keyan Zheng
In this study, we designed and synthesized two novel fluorescent magnetic nanoparticles . Fe 3 O 4 @SiO 2 -NH-GSH-CdTe (FSGC) (GSH = glutathione) nanoparticles were synthesized using amino-functionalized Fe 3 O 4 @SiO 2 nanoparticles and GSH-stabilized CdTe quantum dots (CdTe QDs), while flexible Fe 3 O 4 @SiO 2 -NH-GSH-CdTe-NH-NH 2 (FSGCN) nanoparticles were synthesized using the FSGC precursor and 1,6-hexamethylenediamine. These two kinds of nanoprobes exhibited excellent magnetic and fluorescent properties. By comparing the fluorescence quenching effect of folic acid (FA) on FSGC and FSGCN, we found that the quenching effect of FA on FSGC was acute and the process was too fast to determine the FA content. However, the quenching effect of FA on flexible FSGCN was mild and hence it could be used as a nanoprobe to determine FA concentration. At physiological pH, the fluorescence quenching effect of FA on the FSGCN nanoprobes was fitted according to the Stern–Volmer equation with a linear response in the concentration range of 0.14 to 4.20 μg mL −1 with a detection limit of 15.1 × 10 −9 g mL −1 (S/N = 3) under optimized experimental conditions. The proposed flexible nanoprobe was successfully used to determine the content of FA in folic acid tablets. Recovery was found to be in the range of 92.7%–105.6% with a relative standard deviation of 1.12%–3.84%. Owing to their good stability, environment-friendly characteristics, high selectivity, and good optical properties and biocompatibility, these nanoprobes have potential for usage in practical applications.
研究领域:
生物医学工程
·
分析化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Hong Dai,Yilin Li,Shupei Zhang,Lingshan Gong,Xiuhua Li,Yanyu Lin
Herein, a signal-amplified photoelectrochemical sensing platform based on interwoven titanate nanotubes and carbon nanohorns conjugates was constructed for folic acid determination. Specifically, titanate nanotubes with reticulation structure were employed as optoelectronic element, which possessed fine photocatalytic activity and enhancement in facilitating fast and long-distance electron transport. Hierarchical-structured carbon nanohorns were introduced as the mainstay to provide as the electron-transport medium to capture and transmit electrons from excited titanate nanotubes to the sensing matrix owing to the prominent conductivity and large surface. Furthermore, the holes from valence band of stimulated titanate nanotubes could transfer to their surface and be consumed by folic acid, thus enhancing the photocurrent response. Hence, under the optimal conditions, a linear relationship between photocurrent increase and logarithm concentration of folic acid was obtained in the wide range from 1 × 10 −10 to 5 × 10 −5 M with a detection limit of (2.5 ± 0.005) × 10 −11 M. The well-designed transducer showed practical advantages, such as universality, simplicity and convenience, opening up a valuable application for other important molecules assessment.
研究领域:
分析化学
·
纳米材料科学
·
生物医学工程
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）