Small Methods
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2025
·
IF 9.1
Hang Ao,Wencheng Xiao,Weiwei Chen,Wenrui Hu,Jie Wu,Huangxian Ju
Chemiluminescent immunoassay is the most widely used protein detection technique in clinical diagnosis but still faces substantial challenges in antibody-related single-cell analysis due to the lack of a homogeneous immunoassay with strong and stable chemiluminescence signal. Herein, a single-cell microfluidic platform for efficient in situ detection of antibody secreted from single hybridoma cells through a microcavity chemiluminescent immunoimaging (MCCLII) protocol is reported, which consist of an imidazole-enhanced chemiluminescence system, a proximity-triggered DNA nanomachine and a hybrid-regulated hemin-DNA switch. In MCCLII, antibody secreted from single hybridoma cells can be visualized through a homogeneous proximity CL assay, which converses the target antibody to DNA for triggering the DNA nanomachine and then activates the catalytic activity of hemin-DNA switch to produce strong and persistent chemiluminescence for micro-imaging. The MCCLII realizes immunoimaging detection down to 66 antibody molecules in 0.79-nL microchamber and demonstrates the possibility of specific hybridoma cell screening within 30 min, which provides a simple and fast antibody screening platform to promote antibody-drug production.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2025
·Vol. 36
·期 18
·1-17
·
IF 2.8
Zeng Xiangjian,Pan Zhanchang,Hu Guanghui,Xia Guowei,Huang Haiqing,Zou Mingliang,Shi Shikun
Literature reports indicate that existing synthesis methods for leveling agents often suffer from complex procedures, high environmental burden, challenging purification processes, and insufficient via filling efficiency. To address these limitations, a novel leveling agent (CDT) was synthesized using environmentally friendly materials through a simplified, cost-effective process that eliminates the need for post-synthesis purification. This study systematically investigates the influence of CDT on blind via filling performance in an acidic copper sulfate plating bath containing polyethylene glycol (PEG), bis(3-sulfopropyl) disulfide (SPS), and chloride ions. The chemical structure of CDT was characterized by Fourier-transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), and gel permeation chromatography (GPC). Quantum chemical calculations and molecular dynamics (MD) simulations revealed that CDT exhibits stronger adsorption on copper surfaces compared to conventional leveling agents. Electrochemical measurements confirmed its ability to suppress copper deposition and helped determine the optimal working concentration of CDT. Electroplating experiments further demonstrated CDT’s capability to achieve superfilling, indicating its suitability for printed circuit board (PCB) manufacturing. Scanning electron microscopy (SEM) results corroborated the validity of the optimal concentration established by electrochemical tests. Finally, X-ray photoelectron spectroscopy (XPS) confirmed strong interfacial interactions between CDT and the copper surface. These findings collectively suggest that CDT is a highly promising candidate for advanced leveling agent applications.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
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IF 6.6
Shudong Peng,Jiajun Li,Yating Long,Ying Zheng,Dongming Lan,Yonghua Wang
Foodborne Escherichia coli is one of the health-threatening pathogens existing on the surface of vegetables and fruits. Although antibiotics are the first line of defence against bacteria, increasing concerns are awake about the multidrug resistance caused by overuse and misuse of antibiotics. Therefore, alternative antibacterials are urgently necessary. In this context, we identified, cloned and expressed a novel endolysin LysD6E derived from marine phage D6E for its potential antibacterial action against E. coli . LysD6E consists of an Amidase_3 domain belonging to the N-acetylmuramoyl-L-alanine amidase family. LysD6E was stabile between 4 and 70 °C, and pH between 6 and 10. It had strong lysogenic activity against E. coli and Salmonella typhi . Furthermore, when applying 0.5 μM of LysD6E together with 5 mM EDTA on lettuce for 2 h, viable counts of E. coli ATCC 25922 reduced by 2.05 Log CFU/mL. Our findings suggest that phage-derived LysD6E has the potential against foodborne E. coli in raw vegetables.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 7
·1-12
·
IF 5.3
Wu Han,Li Hai,Shu Jiayi,Yang Changyan,Wang Jianglong,Li Haitao,Shao Min,Zhou Hefeng
In December 2019, Wuhan, China, reported the first cases of pneumonia caused by a previously unknown coronavirus, subsequently designated SARS-CoV-2. This pathogen precipitated a global pandemic of coronavirus disease 2019 (COVID-19). The virus primarily transmits via aerosols, direct contact, and contaminated surfaces. Its high infectivity and rapid transmission have posed severe public health challenges on a global scale, emphasizing the essential need for precise and swift diagnostic techniques to effectively track and curb its transmission. To address this need, we designed a highly efficient approach, namely a colloidal gold immunochromatographic strip (CGICS) based on an IgG-mediated immunoassay, for the specific capture of the SARS-CoV-2 nucleocapsid (N) antigen. Evaluation of the assay’s specificity with a panel of common respiratory viral pathogens revealed that the CGICS specifically recognized SARS-CoV-2, with no cross-reactivity observed against non-target viruses. In addition, limit-of-detection (LOD) assessments indicated that the minimum detectable concentration was 2 ng/mL, and agreement analysis experiments showed a concordance rate of 98%, demonstrating high specificity and sensitivity. The resulting CGICS was capable of detecting SARS-CoV-2 antigen within 5–15 min. This study provides a rapid diagnostic approach for early SARS-CoV-2 infection, offering significant implications for effective disease prevention, control, and clinical diagnosis. Graphical
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JOURNAL OF MEMBRANE SCIENCE
·
2025
·
IF 9
Ning Liu,Jie Wang,Huiyu Yang,Fan Cheng,Shuguang Bi,Guoliang Liu,Ying Ou,Hai Liu,Chunli Gong
In order to improve the proton conductivity, CH 3 OH barrier performance and long-term durability of proton exchange membranes in direct methanol fuel cells (DMFCs), herein, a novel functional nanofiber substrate (IL-ZIF-8@PVDF) was prepared by in situ growing metal-organic framework (ZIF-8) on poly(vinylidene fluoride) (PVDF) nanofibers, followed by modification with ionic liquid (IL) containing sulfonic acid groups. PVDF nanofibers can provide flexible mechanical support for ZIF-8, thereby effectively solving the processing difficulties of ZIF-8 caused by its brittleness. Further IL modification of ZIF-8 can not only increase the compatibility between ZIF-8@PVDF and the filling polymer, but also utilize the continuous PVDF nanofiber network to establish long-distance continuous H + transport pathways within the membrane. The as-prepared sulfonated poly (ether ether ketone) impregnated IL-ZIF-8@PVDF composite membrane showed a very high proton conductivity of 161.92 mS cm −1 (80 °C). Meanwhile, the presence of nanofiber backbone and ZIF-8 inside the membrane effectively limited the swelling and methanol permeation. The peak power density (PPD) of the DMFC output equipped with the membrane is up to 114.86 mW cm −2 (2 M methanol, 80 °C), 1.28 times that of the commercial Nafion 211. The durability test suggested that the composite membrane assembled DMFC possessed excellent open circuit voltage stability (only 6.3 % attenuation after 320 h of continuous testing). This work provides a new design idea for PEMs for DMFCs.
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INDUSTRIAL CROPS AND PRODUCTS
·
2026
·
IF 6.4
Hongkun Xue,Haiyan Gao,Yaojie Liu,Bozhao Zhang,Jianqing Liao,Chuan Wang,Jiaqi Tan
Hangzhou chrysanthemum is a medicinal plant with food-related properties, and the hypoglycemic components of its natural polysaccharides and their related mechanisms are not yet fully understood. This paper purified the crude Hangzhou chrysanthemum polysaccharides (HCPs) via DEAE-52 and Sephadex-G150 column chromatography to finally obtain a major homogeneous polysaccharide fraction (HCPs-3-SG), and then the structure of HCPs-3-SG was characterized by HPGPC, FT–IR, GC-MS NMR, CD, TGA, etc. Finally, the α-glucosidase inhibitory mechanisms of HCPs-3-SG were investigated through comprehensive experiments and simulation methods. The results show that HCPs-3-SG with an Mw of 7.85 kDa consisted of Fuc (1.42 %), Rha (6.89 %), Ara (7.22 %), Gal (5.55 %), Glc (1.67 %), Xyl (2.09 %), Man (1.37 %), and Gal-UA (73.80 %). →4)-α- D -GalUA(6-OCH 3 )-(1→ and →4)-α- D -GalUA-(1→ was the primary chain of HCPs-3-SG. Moreover, HCPs-3-SG didn’t has a triple helix structure and contained microcrystalline structures, irregular fragments, and reticular segment structures. HCPs-3-SG presented a random linear chain, multi-branched structure, and spindle-shaped protrusions. Furthermore, HCPs-3-SG exhibited a strong inhibitory effect on α-glucosidase activity. The α-glucosidase-HCPs-3-SG complex remained stable in terms of RMSD, RMSF, and turnaround radius in the Gromacs simulation. HCPs-3-SG played a crucial role in the binding process with α-glucosidase by interacting with amino acid residues Tyr158, Phe303, Arg315, and Arg442.
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Sustainable Materials and Technologies
·
2026
·
IF 9.7
Qian Wang,Qiuchen Wang,Xuelin Zhang,Yuheng Zhang,Xinyue Chi,Weijian Yuan,Dianpeng Qi,Jianfeng Wu
Selective aerobic oxidation of thioethers to sulfoxides over efficient visible-light photocatalysts is an essential yet highly challenging transformation in catalytic oxidation chemistry. Herein, we introduce an asymmetric perylene diimide–based π-conjugated polymer (BCPDI) with intrinsic polarization that enhances charge transport and tunes the local microenvironment to efficiently regulate reactive oxygen species(ROS) generation for efficient thioether oxidation. DFT calculations show that symmetry breaking separates frontier orbitals and creates an internal electrostatic potential gradient, thereby accelerating charge carrier transport and driving O 2 reduction to generate superoxide radicals (·O 2 − ), whereas symmetric analogs produce only singlet oxygen ( 1 O 2 ) via energy transfer. Driven by the synergistic effect of electron and energy transfer pathways, BCPDI achieved over 99% conversion and selectivity for thioether oxidation under ambient conditions, outperforming most recently reported materials. In addition, BCPDI also exhibits a superior catalytic activity for thioether oxidation (e.g., aromatic thioethers, aliphatic sulfide, and Mustard Gas sulfide). This study provides a new approach for the design of catalysts for photocatalytic aerobic oxidation of thioethers and deepens the understanding of thioether activation mechanisms mediated by photogenerated charge carriers and reactive oxygen species.
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LANGMUIR
·
2026
·
IF 4.4
Cong Xiang,Fan Xue,Shunzong Wu,Juan Wang,Chenyu Yang,Yike Huang,Xiangzi Li,Meifang Wang,Xiaomei Cheng
Histidine-rich proteins (His-proteins) are vital biomolecules with diverse physiological roles. However, their purification from complex biological samples remains challenging. Here, we successfully prepared Ni/NixPy composite nanospheres with different phosphating degrees, featuring surface-exposing Ni2+ properties for efficient separation and purification of His-proteins. The phosphated products exhibited a spherical morphology, reduced cytotoxicity, and good biocompatibility. The optimal Ni/NixPy demonstrated excellent adsorption and separation capacity for His-tagged bovine hemoglobin (BHb), which is attributed to their Ni2+ surface-exposing capability and magnetic properties, achieving a adsorption capacity of 2130.4 mg/g under the tested conditions. While the adsorption sites for His-proteins are enhanced, the separation process is simplified. SDS-PAGE analysis confirmed the selective adsorption of Ni/NixPy for His-proteins in a BSA/BHb mixed solution. The Ni/NixPy spheres demonstrated good stability and recyclability, maintaining an 85% adsorption efficiency after five cycles. In addition, His-proteins can be efficiently separated and purified from complex fetal bovine serum. Therefore, this work reports an effective surface-exposing Ni2+ approach to enhance the efficiency of protein separation and purification with the potential to expand applications in diagnostics and therapeutics.
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Separations
·
2026
·Vol. 13
·期 2
·72
·
IF 3.5
Mengshuai Liu,Xiaoman Li,Mengmeng Zhao,Xuyang Jiu,Chuang Yao,Minglei Tian
Background: Food waste contains abundant (+)-catechin, but its efficient recovery remains challenging. This study aimed to prepare ionic liquid (IL)-modified sorbents and establish an efficient method for (+)-catechin recovery from chocolate waste via solid-phase extraction (SPE). Methods: Three series of IL-modified sorbents (Sil-IL, ZIF67-IL, Sil@ZIF67-IL) were synthesized. Their adsorption performance was evaluated under different conditions; adsorption isotherm and kinetic data were fitted to Langmuir/Freundlich and pseudo-first/second-order models, respectively. Sorbent stability and (+)-catechin recovery from chocolate waste extracts were tested. Results: Sil@ZIF67-Hmim showed the highest adsorption capacity (154.4 mg/g) at 25 °C within 120 min. Adsorption followed the Langmuir model (R2= 0.99), indicating chemisorption. Sil@ZIF67-Hmim was subjected to repeated solid-phase extraction (SPE) for five consecutive days; the recovery rate ranged from 98.1% to 99.2%, and the relative standard deviation (RSD) was 3.2–4.4%. Conclusions: Sil@ZIF67-Hmim is a high-efficiency sorbent for (+)-catechin recovery from chocolate waste, providing a novel approach for food waste valorization and highlighting the application potential of IL-modified MOF-silica composites.
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Analytical Methods
·
2026
·
IF 3
Ruoran Shi,Jingyao Liu,Honglin Ren,Yansong Li,Jiaqi Hou,Shaohui Hu,Yuzhu Wang,Chengwei Li,Bo Zhang,Ziqi Xing,Yuyang Ding,Fang Yang,Shiying Lu,Qiang Lu,Zengshan Liu,Xiaoxu Wang,Pan Hu
Listeria monocytogenes (LM) is one of the eight species within the Listeria genus and one of the most lethal foodborne pathogens. Accurate and rapid detection is crucial for preventing infections caused by this bacterium. Nanobodies, with the advantages of strong affinity, high specificity, low off-target effects, excellent thermal stability, and good solubility, have emerged as a novel reagent for pathogen detection. InlG (Internalin G), a surface-anchored virulence protein accessible on the bacterial surface, was selected as the target antigen to ensure high specificity and effective antibody binding. This study expressed and purified the inlG protein by prokaryotic expression. High-specificity nanobodies targeting the inlG protein were screened using phage display technology and were then used as capture antibodies to establish an indirect sandwich ELISA detection method. The established indirect sandwich ELISA assay demonstrates excellent reproducibility (intra-assay CV: 2.39% and inter-assay CV: 2.78%) and high specificity, with slight cross-reaction with Escherichia coli, and no significant cross-reaction with other food-borne pathogens. The limit of detection (LOD) was 1.0 × 105 CFU mL−1 and limit of quantitation (LOQ) was 3.0 × 105 CFU mL−1 (S/N = 10, CV ≤ 10%). Spiked recovery rates ranged from 96.0% to 107.5% in five food matrices (beef, fish, milk, soft cheese, and deli turkey), with an RSD ≤ 4.68%. Furthermore, these nanobodies targeting InlG may serve as penicillin for controlling L. monocytogenes infections, and chemical residues benzylpenicilloic acid (BPNLA) risk, addressing the challenge of antibiotic resistance.
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CHEMISTRY OF MATERIALS
·
2026
·
IF 7.1
Chaohui Guo,Ang Qiao,Xuan Ge,Xiaoming Ren,Zijuan Du,Haizheng Tao,Yuanzheng Yue
Modulating the degree of structural disorder in metal–organic frameworks (MOFs) offers a promising strategy for tuning their physical and chemical properties. Structural disorder in MOFs can be created by various means, e.g., mechanical treatment or melting. Here, we show that structural disorder can be induced in an iron-based zeolitic imidazolate framework (denoted as Fe-ZIF) upon dynamic heating. The onset temperature of such structural disorder in Fe-ZIF is lower than its melting point. Small- and wide-angle X-ray scattering, together with high-energy synchrotron X-ray diffraction, revealed that structural disorder primarily evolves at the medium- and long-range scales, while the short-range order remains largely preserved. The distortion of medium-range structural domains, particularly the [Fe4Im8] clusters, led to the loss of long-range atomic periodicity in Fe-ZIF. Moreover, magnetic measurements demonstrate that the sample with a higher degree of medium-range structural disorder exhibited a lower spin freezing temperature. Thus, our work established a viable strategy for tailoring structural disorder in MOFs, enabling precise modulation of their magnetic functions.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
·
IF 5.4
Qian Zhang,Hao-Zhe Li,Bing Li,Sha Li
The efficient removal of emerging anionic organic pollutants, particularly pharmaceuticals and herbicides such as sodium diclofenac (DCF-Na) and sodium 2,4-dichlorophenoxyacetate (2,4- D -Na), from aqueous environments remains a significant challenge. Although hydrochar is a sustainable and low-cost adsorbent, its intrinsic negative surface limits its affinity for anionic contaminants. To overcome this, a novel mesoporous poly(imidazolium)-grafted hydrochar (PIHC) was synthesized via a one-pot in situ quaternization polymerization by directly grafting 1,3,5-tris(1H-imidazol-1-yl)benzene and 1,3,5-tris(bromomethyl)benzene onto bamboo hydrochar. The impact of aqueous pH, adsorbing temperature, ionic strength and contact time on the removal of DCF-Na and 2,4- D -Na by PIHC was systematically investigated. The resulting PIHC exhibited rapid and efficient adsorption, removing 52 % of DCF-Na (C 0 = 400 mg·L −1 ) and 88 % of 2,4- D -Na (C 0 = 100 mg·L −1 ) within 5 min. The adsorption kinetic and isotherm data fitted well with the pseudo-second-order kinetic model and the Langmuir model, indicating that the adsorption was dominated by monolayer chemical adsorption. Thermodynamic test uncovered a spontaneous and endothermic adsorption, with the spontaneity mainly driven by entropy. The high performance is attributed to the synergistic effects of the mesoporous structure and high positive charge density, enabling multiple mechanisms including electrostatic attraction, ion exchange, and π-π interactions. This work provides a versatile molecular design strategy for developing high-performance, imidazolium-grafted hydrochar for pharmaceutical and herbicide remediation.
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