JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Xinjian Bi,Ruibo Huang,Tingmin Qu,Ying Wu,Hao Wu,Daichen Mu,Jian Hu,Juan Liao,Yanting Duan,Li Wen
This study identified uric acid-lowering peptides from rice residue protein hydrolysates following simulated gastrointestinal digestion. Using ultrafiltration, LC–MS/MS, bioinformatics, and molecular docking, two peptides─FGHPEW (No. 56) and FFAFGR (No. 117)─were screened and validated for their biological activity. Both peptides showed notable in vitro xanthine oxidase (XOD) inhibition (IC50: 476.3 and 673.8 μg/mL, respectively). In zebrafish models of hyperuricemia, they helped restore uric acid balance and improved kidney function. Mechanistically, the peptides inhibited XOD, downregulated Glut9, and upregulated Oat1 expression, contributing to enhanced uric acid excretion. Peptide 117 additionally activated Nrf2 and showed the potential to inhibit uric acid crystallization. Molecular docking and dynamics simulations confirmed strong, stable binding of both peptides to target receptors via hydrogen bonding and hydrophobic interactions. These results suggest that peptides 56 and 117 lower uric acid through multiple pathways and may serve as promising therapeutic candidates for hyperuricemia.
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MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Qian Bi,Hongmei Zhang,Han He,Qian Li,Baoting Dou
Doxorubicin (DOX) is a frequently prescribed chemotherapy medication for a range of cancers. It is essential to monitor the levels of doxorubicin in human biological fluids to ensure effective treatment. An efficient electrochemical sensor for amplified detection of DOX is developed in this work. The strand displacement reaction is initiated when the blocking probe, which attaches to the terminal toehold sequence of the immobilization probe, is released due to the selective recognition of DOX with the aptamer probe. The secondary toehold region is then available for hybridization with the methylene blue (MB)-labeled signal probe, causing the blocking probe to be displaced and activating the molecular machine. Consequently, the blocking probe is reused in a cyclical manner, with numerous signal probes being attached to the sensor surface, resulting in a significant increase in current for quantitative analysis. This technique successfully detected DOX in human serum samples with high selectivity and sensitivity, reaching a detection limit of 670 fM. The proposed research offers a novel approach to identifying various biomarkers, with the potential to significantly advance clinical diagnosis and drug research.
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MICROCHEMICAL JOURNAL
·
2025
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IF 5.1
Rui Yao,Zhenyu Wu,Olanrewaju Yaasir Olatunji,Khaydar E. Yunusov,Fozil Mamarayim Ugli Turakulov,Jing Chen,Guohua Jiang
Although subcutaneous implanted continuous glucose monitoring (CGM) systems have proven effective in promoting diabetes management, their adoption has been limited because of high production costs and reduced accuracy and precision resulting from their invasive nature. In this research, a biosensor based on a minimally invasive solid microneedle array for continuous glucose monitoring was introduced. The device was consisted of solid metal microneedles integrated with polydopamine (PDA) and reduced graphene oxide (rGO) to immobilize platinum nanoparticles (Pt NPs)s. The geometric features of these microneedles allowed them to penetrate dermal tissue without the need for an applicator. The as-fabricated biosensor has been used for blood glucose detection as a non-enzymatic electrochemical biosensor with a wide detection range of 2–38 mM, high sensitivity of 0.48 μA/mM/cm 2 and low detection limit of 0.18 mM. To assess its practical applicability, the efficacy of the biosensor was evaluated on in vivo animal assessment. The results demonstrated a high degree of consistency in blood glucose levels between those detected by the developed microneedle biosensor and the conventional glucometer.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2025
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IF 9.7
Lin Jiang,Liting Yao,Fan Wu,Chenjiang Liu,Weiwei Jin,Qi Ao,Maofeng Wang
Glucose offers basic power for the daily activities of human beings, and the concentration of glucose in serum influences health. At present, the main problems faced by nanogel glucose detection include: the sensitivity and selectivity need to be further improved, and the long-term in vivo stability is insufficient. Here, a cyclodextrin-based multi-functional nanogel was synthesized for glucose detection. In such a nanogel, glucose oxidase (GOx), chitosan (CS) and β-cyclodextrin (β-CD) were cross-linked in the nanogel net and Fe ions coordinate with multiple groups and cyclodextrin lumen. A cascade catalysis of GOx and Fe nanoparticle could enhance the detection sensitivity for better sensitivity. 1 H nuclear magnetic resonance result confirmed that β-CD has a host-guest interaction with the substrate glucose for better catalysis with GOx for selectivity. Moreover, the Fe coordination with nanogel make the structure stable for long-term detection. Overall, the Fe-based nanogel strategy provides a new glucose detection method.
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TALANTA
·
2025
·
IF 6.1
Xiwang Hou,Tianhao Chen,Chunyan Wang,Fanyu Liu,Minggang Zhao,Ye Ma
The concentration range of uric acid (UA) varies significantly across different biological fluids, and therefore, highly sensitive and wide-range detection of UA is of great importance for the early diagnosis of UA-related diseases. Surface-enhanced Raman spectroscopy (SERS) offers high sensitivity and molecular fingerprint specificity; however, challenges remain regarding its signal uniformity and detection range. In this work, a uniform and stable two-dimensional metasurface is constructed via liquid–liquid interface (LLI) self-assembly of silver nanoparticles (NPs) functionalized with bis (p-sulfonatophenyl) phenylphosphine di-potassium salt (BSPP). UA is catalytically oxidized by uricase, which in turn oxidizes the BSPP ligands on the NP surface, inducing microstructural changes in the metasurface, which led to a decrease in the BSPP signal in the SERS mode at low UA concentrations and a reduction in reflectance in the reflectance mode at high UA concentrations. Using this dual-mode strategy, a detection limit (LOD) of 3.22 ± 0.10 nM for UA and a dynamic range of 0.100–1500 μM are achieved. The strategy enables rapid NPs assembly and complementary linear ranges, offering a versatile approach with potential for extension to diverse analytes and portable point-of-care applications, highlighting its potential significance for the early diagnosis of UA–related diseases.
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IEEE SENSORS JOURNAL
·
2025
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IF 4.5
Fengjuan Miao,Yanan Lu,Bairui Tao
Nanomaterial-based electrochemical sensors enable real-time detection of chemical and biological parameters, with advantages including cost-effectiveness, high selectivity, high sensitivity, and portability. However, their detection sensitivity is drastically reduced in liquids due to the Debye screening effect. This paper investigates a Co₃O₄/CuS/rGO electrode for glucose detection: Co₃O₄ is obtained via annealing, while CuS and 3D wrinkled graphene are synthesized by hydrothermal method. Polyvinylpyrrolidone (PVP) is used during reduced graphene oxide (rGO) preparation to prevent graphene oxide sheet aggregation. Additionally, adding a biomolecule-permeable, porous polymer layer to the sensor extends the effective screening length near the device surface, enabling real-time glucose detection in high-ionic-strength fluids. The sensor exhibits a glucose detection range of 0.10–500 μM, sensitivity of 84.18 μA·mM⁻¹·cm⁻², signal-to-noise ratio (SNR) of 3, and detection limit of 0.046 μM. This study provides a valuable insight for applying nanomaterial-based electrochemical sensors in sweat glucose detection, which could become a powerful tool for basic research and medical applications.
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Tungsten
·
2025
·
IF 11.2
Wang Xue,Zhang Li,Han Yu,Li Feng-Bo,Li Shao-Bin
Accurate non-invasive glucose monitoring is crucial for health diagnostics. However, the development of high-performance non-enzymatic electrochemical sensors for this application remains a significant challenge. To address this issue, a novel material, FeOOH encapsulated within zeolitic imidazolate framework-67 (ZIF-67)-derived copper-cobalt-layered double hydroxide (FeOOH@CuCo-LDH), was developed through a vapor-assisted deprotonation process for FeOOH encapsulation, followed by a hydrothermal reaction to synthesize the hollow composite. The synergistic interactions between the multi-metallic composition and the hollow structure significantly enhance glucose oxidation-reduction reactions. This enables the sensor to achieve a wide linear detection range (0.5–722 μmol·L −1 and 722–4222 μmol·L −1 ), high sensitivity (4.05 and 1.43 μA·L·μmol −1 ·cm −2 ), and a low limit of detection (0.83 μmol·L −1 ). The sensor demonstrated excellent performance in practical applications, accurately detecting glucose in saliva under physiological conditions. This study highlights the potential of FeOOH@CuCo-LDH as an excellent electrode material for non-enzymatic electrochemical glucose sensing, offering promising applications in diabetes prevention and diagnosis. Graphical abstract
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xuyuan Sun,Minmin Liu,Ying Wang,Zhengyuan Dai,Li Li,Yaping Ding
A molecularly imprinted electrochemical sensor (MIECS) based on dual functional monomers was designed for the trace analysis of ramipril (RAM). The molecularly imprinted polymer (MIP) film was prepared by electropolymerization, p-aminobenzoic acid (p-ABA) and 2-amino-5-mercapto-1,3,4-thiadiazole (AMT) were used as dual functional monomers, and RAM was used as template molecule. Compared with sensors that only use a single functional monomer (p-ABA or AMT), the synergistic effect of dual functional monomers enhances the selectivity of MIECS. Under optimal conditions, the MIECS demonstrated a low detection limit of 23.9 fM within a wide linear range from 10 −13 M to 10 −6 M. Its application was validated by the successful detection of RAM in tablets with satisfactory recovery.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 7
·1-11
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IF 5.3
Kuang Xia,Zhao Guocai,Guo Jingyi,Tang Qin,Yu Jun,Wang Fang
Harnessing the exceptional enzyme immobilization capabilities of metal–organic frameworks (MOFs) and the exceptional electrochemical characteristics of graphene, we designed a highly efficient and cost-effective glucose sensor utilizing zeolitic imidazolate framework-8 (ZIF-8) and laser-induced graphene (LIG) with the aid of mediator ferrocene (Fc). By incorporating a cellulose acetate (CA) film as an anti-interference layer and a polyurethane (PU) film as a diffusion-limiting layer, the sensor exhibited a wide linear detection range for glucose (0.62 mM to 20 mM) with a low detection limit of 0.16 mM (S/N = 3). Notably, it demonstrated excellent selectivity, with no interference from common biological substances. The sensor also exhibited good stability, retaining 96.38% of its initial performance after 7 days of storage. Additionally, the sensor was effectively utilized for glucose detection in whole blood, with results closely matching those obtained from a commercial blood gas analyzer (GEM5000), as evidenced by a high Pearson's correlation coefficient (r = 0.9974). This method provides a portable glucose sensor that produces results highly consistent with those obtained from large-scale testing devices in hospitals, enabling convenient and accurate blood glucose monitoring for patients. Graphical
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 7
·1-14
·
IF 5.3
Gao Yueming,Weng Guojun,Li Jianjun,Zhu Jian,Zhao Junwu
A flexible silver triangular nanoplate (Ag TNPs)@m-phenylenediamine (mPD)@polyacrylonitrile (PAN) skin patch based on electrospinning is fabricated and applied to detect human beta-defensin 2 (HBD-2) by using surface-enhanced Raman scattering (SERS). First, PAN was used as the base material to create a fibrous membrane through electrospinning. The fibers were then modified with Ag TNPs by mPD endowing positive charges, forming a flexible Ag TNPs@mPD@PAN substrate. The flexible substrate features advantages of simple preparation, low cost, and convenient portability, which are suitable for in situ rapid detection on irregular surfaces. The combination of the flexible Ag TNPs@mPD@PAN substrate with Ag TNPs probes enabled the specific detection of HBD-2. It demonstrated satisfactory sensitivity and reproducibility with a wide detection range of 100–10 ng/mL and a limit of detection (LOD) of 59.57 fg/mL. Eventually, the flexible substrate was applied to HBD-2 detection on the surface of contaminated chicken skin. This study provides a simple and highly sensitive method for the detection of HBD-2, offering a novel approach for the non-invasive rapid diagnosis of psoriasis and the monitoring of disease progression. Graphical
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BIOELECTROCHEMISTRY
·
2025
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IF 4.5
Jiamu Li,Aijia Sun,Han Lai,Chuanhe Li,Huayi Li,Zhengchun Yang,Peng Pan,Jie He,Rui Zhang,Chunhong Wang
In this study, an electrochemical sensor for the specific detection of Pseudomonas aeruginosa ( P. aeruginosa ) was developed using an F23 aptamer-functionalized nitrogen-doped multi-walled carbon nanotubes (N doped-MWCNTs) and silver nanoparticles (AgNPs) composite. Systematic optimization of the Ag/C ratio revealed that a 1:10 composition delivers superior electrochemical performance, owing to synergistic effects between highly dispersed AgNPs and efficient nitrogen doping. Then a biosensor was constructed based on a three-electrode system, featuring a screen-printed electrode (SPE) modified with optimized N-doped MWCNTs/AgNPs-10/F23 aptamer as the working electrode. The structural and compositional characteristics of the sensor materials were systematically characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS), and X-ray photoelectron spectroscopy (XPS). The electrochemical performance was evaluated through cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) to assess the conductivity and charge transfer properties of the electrode materials. The sensor exhibited a wide linear detection range from 10 −1 to 10 6 CFU·mL −1 and the limit of detection is 0.0798 CFU·mL −1 , demonstrating high specificity and sensitivity for P. aeruginosa . This study demonstrates a novel strategy for developing cost-effective, portable biosensors with exceptional selectivity for bacterial pathogen detection, offering significant potential for real-time environmental monitoring and point-of-care diagnostic applications.
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BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Zhenjie Zhang,Wei Wei,Anne Ailina Li,Chenhao Wang,Chengchen Tang,Qing Wang,Lianqun Zhou,Hui Kong
Here, we report a wearable device integrating a sweat collection unit and a molecularly imprinted polymer (MIP) - based sensor for the non-invasive detection of adrenaline (AD). In this paper, a new MIP method is proposed, which uses AD molecule as template, and electropolymerizes aniline and 3-aminophenylboronic acid (3-APBA) to form molecular imprinting. Surface modifications, incorporating carbon nanotubes (CNT) and MIP optimization, enhanced the device's specific surface area by 166.45% (± 2.82%), yielding a wide linear detection range of AD (10 -6 μmol L -1 to 10 3 μmol L -1 ). In this study, a three-dimensional response model was constructed by analyzing the current values corresponding to sweat pH levels (4.0-8.0) and AD concentrations, enabling a linear response and accurate prediction of AD levels in sweat. The integrated flexible electronic platform supports real-time data transmission via Bluetooth or NFC to a mobile application, facilitating efficient detection. Comprehensive integration and validation, including artificial sweat testing and correlation with ELISA results, underscore the sensor’s potential as a robust and versatile solution for wearable health monitoring.
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