JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2014
·Vol. 95
·期 5
·915-921
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IF 1.879
Liang Dong,Yingmin Hou,Feng Li,Yongzhe Piao,Xiao Zhang,Xiaoyu Zhang,Cheng Li,Changxin Zhao
BACKGROUND Beer is a popular alcoholic malt beverage resulting from fermentation of the aqueous extract of malted barley with hops. The aroma of brewing barley impacts the flavor of beer indirectly, because some flavor compounds or their precursors in beer come from the barley. The objectives of this research were to study volatile profiles and to characterize odor-active compounds of brewing barley in order to determine the variability of the aroma composition among different brewing barley cultivars. RESULTS Forty-one volatiles comprising aldehydes, ketones, alcohols, organic acids, aromatic compounds and furans were identified using solid phase microextraction combined with gas chromatography/mass spectrometry, among which aldehydes, alcohols and ketones were quantitatively in greatest abundance. Quantitative measurements performed by means of solvent extraction and calculation of odor activity values revealed that acetaldehyde, 2-methylpropanal, 3-methylbutanal, 2-methylbutanal, hexanal, heptanal, octanal, nonanal, 3-methyl-1-butanol, cyclopentanol, 2,3-butanedione, 2,3-pentanedione, 2-heptanone, acetic acid, ethyl acetate, 2-pentylfuran and benzeneacetaldehyde, whose concentrations exceeded their odor thresholds, could be considered as odor-active compounds of brewing barley. CONCLUSION Principal component analysis was employed to evaluate the differences among cultivars. The results demonstrated that the volatile profile based on the concentrations of aroma compounds enabled good differentiation of most barley cultivars. © 2014 Society of Chemical Industry
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CATALYSIS COMMUNICATIONS
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2013
·
IF 2.915
Hong-De Yan,Qun Zhang,Zhao Wang
A lipase from Aspergillus oryzae WZ007 exhibited high esterification activities toward a series of short-chain acids and alcohols. Moreover, it displayed a strong tolerance for high substrate concentration of up to 2.0 M and presented a highest initial rate of 276 mmol·L − 1 ·h − 1 at this concentration. After a reaction time of 48 h, the conversion rates of acids were higher than 80% for the synthesis of a majority of heptanoic acid esters, some octanoic acid esters and n -propyl hexanoate. These results implied that A. oryzae WZ007 lipase was a promising biocatalyst in the production of flavor esters.
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MICROCHEMICAL JOURNAL
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2024
·
IF 4.9
Mengyao Gao,Chen Yan,Na Li,Yun Wei
Eugenol has many biological and pharmacological activities such as antioxidant, anticancer, anti-inflammatory, antibacterial and anesthetic. In view of its wide range of beneficial activities, it is crucial to establish a method for the separation and purification of active components from clove. In this study, an electroosmotic coupled with particle-assisted deep eutectic solvent flotation method was established for the enrichment of eugenol from clove. Ten deep eutectic solvent (DES) extraction systems based on menthol as the hydrogen bond acceptor (HBA) were screened, and butanol was determined as the hydrogen bond donor (HBD) to prepare the optimal DES as extractant for extracting eugenol. Under the optimum operating conditions including solid–liquid ratio of 1:30, flotation time of 50 min, nitrogen flow rate of 55 mL/min, salt content of 20 g, pH of the clove suspension of 5.2, applied voltage of 15 V, copper electrode at the bottom, the yield of eugenol was 45.89 ± 1.61 mg/g. Compared with other extraction methods, the extraction rate was greatly improved. The electroosmotic coupled with particle-assisted deep eutectic solvent flotation method provides a green and efficient enrichment strategy for natural product, which has broad application prospects.
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FOOD CHEMISTRY
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2024
·
IF 8.5
Xiaojiao Zheng,Xingle Guo,Yawen Zhang,Jiajun Han,Xu Jing,Junxue Wu
A novel, environmentally friendly, and efficient method for determining triazine herbicides in water, tea, and juice was developed by combining magnetic dispersive micro-solid phase extraction (MD-μSPE) with magnetic dispersive liquid-liquid microextraction (MDLLME), followed by high-performance liquid chromatography (HPLC). The pretreatment process, utilized magnetic biochar (MBC) and magnetic deep eutectic solvent (MDES) as the adsorbent and extractant, respectively. Fe(NO 3 ) 3 was loaded onto waste mushroom sticks to prepare MBC via impregnation-pyrolysis, while tri- n -butylphosphine oxide, nonanoic acid, and FeCl 3 were combined through hydrogen bonds to form MDES. This approach significantly reduced environmental impact using waste materials and shortened separation times. The method demonstrated excellent linearity (1–100 μg L −1 ), low detection limits (0.3 μg L −1 ), and good precision (RSDs <6.5 %). Recoveries in water, tea, and juice ranged from 80.1 % to 90.6 %. This combined MD-μSPE-MDLLME approach offers a greener and more efficient extraction process for separating and concentrating pesticides from complex food samples.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Shijiao Fan,Zechuan Lu,Zhenning Yan,Liuyang Hu
In this paper, the interaction of three berberine mid-chain fatty acid salts ([BBR][FAs]), viz. berberine caproate ([BBR][CAP]), berberine heptylate ([BBR][HEP]) and berberine octoate ([BBR][OCT]), with bovine serum albumin (BSA) was studied by means of UV–visible absorption spectroscopy , fluorescence spectroscopy, fourier transform infrared spectroscopy (FT-IR) and molecular docking techniques. Fluorescence experiments revealed that three berberine salts quench the fluorescence of BSA by static quenching mechanism resulted from a stable [BBR][FAs]-BSA complex formation. The stoichiometric numbers of [BBR][FAs]-BSA complexes were found to be 1:1. Synchronous and three-dimensional fluorescence spectra as well as FT-IR demonstrated that the binding of [BBR][FAs] altered the microenvironment and conformation of BSA. The binding average distance from [BBR][FAs] to BSA (3.2–3.5 nm) was determined according to Förster energy transfer theory. Site probe investigation showed that [BBR][FAs] bound to BSA active site I (sub-domain IIA). The binding promotes the esterase-like activity of BSA. The molecular docking results confirmed the fluorescence competition findings and provided the type of binding forces. Furthermore, the relationship between the anionic chain length of [BBR][FAs] and the interaction was explored, and the positive correlation was found.
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Food Bioscience
·
2025
·
IF 5.9
Lin Yuan,Yunjie Li,Liuyan Zheng,Yifan Qin,Xin Zhang,Lijuan Ma,Huan Zhang,Liping Du
Organic acids are critical flavor contributors in light-flavor Baijiu (LFB). The binding of organic acids and volatile compounds may affect the release of flavor in Baijiu. In this study, the effects of different concentrations of lactic acid, hexanoic acid, butyric acid, and acetic acid on the release of key aroma-active compounds in LFB were investigated. Twenty volatile compounds were identified as key aroma-active compounds in four LFB samples using odor activity values combined with aroma recombination, and omission experiment methods. Furthermore, as the concentrations of organic acids were increased in the simulated LFB solution, significant changes in the release of key aroma-active compounds occurred. The addition of lactic acid from 0.5-fold to 5-fold resulted in all key aroma-active compounds exhibiting stronger releases, especially the addition of a 3-fold concentration of lactic increased the release of key esters to about twice. In contrast, the effect of hexanoic acid, butyric acid, and acetic acid on the release of key aroma-active compounds showed a concentration-dependent trend. This suggests that the specific structures of volatiles and organic acids alter the intermolecular interactions between them, thereby altering the aroma release. The results reveal that different organic acids alter the overall aroma profile by affecting the release of volatile compounds in the LFB matrix.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2024
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IF 6
Yiran Bian,Yi Zhang,Taosuo Wang,Chuang Yang,Zhiming Feng,Kheng-Lim Goh,Yibin Zhou,Mingming Zheng
The enzymatic synthesis is essential for the flavor esters in the food and fragrance industries. This paper introduces a novel preparation method for lipase microarrays (CALB@PMHOS-TEOS) with loadings up to 229 ± 1.4 mg/g. Using surfactant-free hydrophobic silica-hybridized mesoporous materials and Candida antarctica lipase, this resulted in the effective synthesis of flavor esters. Using CALB@PMHOS-TEOS a Pickering emulsion system was formed at the oil-water interface for the sustainable synthesis of flavor esters. This resulted in a 93.5 ± 0.5 % conversion of hexanoic acid within 2 h at an optimal temperature of 35 °C, which is the highest level recorded in the literature to date. Furthermore, the conversion of hexanoic acid was maintained at 63.9 ± 1.2 % after 9 cycles of CALB@PMHOS-TEOS reuse. The application of the enzyme to the synthesis in a variety of flavor esters achieved a new benchmark in the existing literature. A molecular docking model was evaluated to understand the molecular mechanism underpinning the immobilized lipase. This work introduces a novel method for the eco-friendly and efficient synthesis of flavor esters for applications across various fields including food and cosmetics.
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JOURNAL OF CHROMATOGRAPHY A
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2024
·
IF 3.8
Yanhui Zhang,Hongbo Li,Xiaoqin Hai,Xiaoli Guo,Xin Di
A green and recyclable switchable supramolecular deep eutectic solvent (SS-DES) was designed and prepared for effective extraction of flavonoids from Scutellariae Radix . The novel SS-DES has both excellent extraction performance of DES and the host guest inclusion of cyclodextrin, thereby showing superior extraction efficiency and selectivity. The characteristic of polarity switching can endow the SS-DES with achieving homogeneous extraction and rapid two-phase separation, shorting per-treatment time largely. Parameters affecting the extraction performance were investigated by the response surface methodology. The results indicated that the SS-DES showed better extraction yield of total flavonoids (157.95 mg/g) compared with pure DES (135 mg/g) and traditional organic solvent (60 % ethanol, 104.87 mg/g). Moreover, the switching mechanism of SS-DES was characterized by FT-IR and 1 H NMR, and the extraction mechanism was studied by density functional theory and molecular docking analysis. After evaluating the ecological impact of the method, the cytotoxicity of SS-DES was investigated and the result displayed that its toxicity was very low or even negligible with the EC 50 >2000 mg/L. After being adsorbed by macroporous AB-8 resin, the regenerated SS-DES was recycled 5 times and the extraction efficiency still remained above 90 %, indicating the desirable reusability. Therefore, the proposed method was efficient and sustainable, and revealed favorable application prospect for the extraction of bio-active compounds from plant materials.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2024
·
IF 4
Min Wang,Yajie Yue,Jingjing Zhang,Yifan Qin,Liyan Jia,Xu Jing
In this study, magnetic deep eutectic solvent-dispersive liquid-liquid microextraction combined with high-performance liquid chromatography method was developed for the extraction and determination of succinate dehydrogenase inhibitors in water, juice, wine, and vinegar . A new green magnetic deep eutectic solvent was synthesized using quaternary phosphorus salt, metal chloride, and fatty acid. The special self-dispersion magnetic deep eutectic solvent achieved rapid extraction without auxiliary dispersion and separation via neodymium magnets without centrifugation, avoiding the use of toxic disperser solvents and disperser instruments and reducing the separation time. The recovery of succinate dehydrogenase inhibitors under optimal extraction conditions was 76.5–97.8 %, and the limit of detection was 0.0011–0.0029 mg L −1 . The method could be a fast, simple, green, and reliable alternative for the analysis of succinate dehydrogenase inhibitors. The self-dispersion magnetic deep eutectic solvent can facilitate the development of dispersive liquid-liquid microextraction methods of other pesticides in food samples.
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CHEMICAL ENGINEERING COMMUNICATIONS
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2025
·
IF 2
Alvin H. H. Teo,S. Y. Chin,Fiona W. M. Ling,Hayder A. Abdulbari
Water shortage is an imminent issue in the foreseeable future while thermal desalination techniques are energy intensive. This study investigates a membrane-less low temperature technique known as directional solvent extraction (DSE) desalination in a serpentine microchannel at 45, 50, 55, 60 and 65 °C. Two lower fatty acids, heptanoic acid (HeptA) and nonanoic acid (NonA) were used as the solvents to recover water from a 35000 ppm total dissolved solids (TDS) seawater at 2, 3, 4 and 5 organic to aqueous flowrate ratios (O: aq). Custom-made polydimethylsiloxane (PDMS) – glass microfluidic device is fabricated via direct writing lithography with depth and length of 100 µm and 161 mm respectively while the width is varied from 400 - 800 µm. Droplets were generated during the extraction process to maximize diffusion by using a 90° T-junction where the organic solvent is the continuous phase and seawater is the dispersed phase. A maximum product water yield, Yw of 4 % v/v and 1.9 % v/v is achieved by HeptA and NonA respectively. The product water recovered from HeptA and NonA has salinity as low as 0.01% and 0.08%, respectively. The HeptA system produces water containing higher solvent residuals than the NonA system. HeptA is a better solvent as it can extract approximately two times more water than NonA under the same operating conditions. Both solvents are reusable.
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Thoracic Cancer
·
2020
·Vol. 11
·期 6
·1476-1486
·
IF 2.61
Kun Zheng,Runze Chen,Yanxue Sun,Zhenquan Tan,Ye Liu,Xiao Cheng,Junke Leng,Zhaoming Guo,Pengcheng Xu
Background Although photodynamic therapy (PDT) has emerged as a potential alternative to conventional chemotherapy, the low reactive oxygen species (ROS) yield of the photosensitizer such as TiO 2 nanoparticles has limited its application. In addition, it is difficult to achieve effective tumor treatment with a single tumor therapy. Methods We used TiOx nanocomposite (YSA-PEG-TiO X ) instead of TiO 2 as a photosensitizer to solve the problem of insufficient ROS generation in PDT. Benefiting from the desired mesoporous structure of TiOx, Cantharidin (CTD), one of the active components of mylabris, is loaded into TiOx for targeted combination of chemotherapy and PDT. The cellular uptake in human non-small cell lung carcinoma cell line (A549) and human normal breast cell line (MCF 10A) was evaluated by confocal microscopy. in vitro cytotoxicity was evaluated using Cell Counting Kit-8 assay. The ROS was detected via a chemical probe DCFH-DA and the photodynamic treatment effect of YSA-PEG-TiOx was further evaluated by a living-dead staining. The cell apoptosis was detected by the flow cytometry. Results Our findings showed that the modification of YSA peptide improved the cytotoxicity of YSA-PEG-TiO X /CTD to EphA2 overexpressing A549 non-small cell lung cancer (NSCLC) than non-YSA modified counterparts. In addition, TiOx generated adequate ROS under X-ray irradiation to further kill cancer cells. Flow analysis results also proved the superiority of this combined treatment. Conclusions YSA-PEG-TiO X nanoparticles could significantly increase ROS production under X-ray exposure and provide a new drug delivery nanocarrier for CTD in combination with PDT to achieve effective NSCLC treatment.
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FOOD CHEMISTRY
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2025
·
IF 9.8
Ruili Ma,Weiguo Wu,Yikang Shu,Ran Gao,Ziyu Wang,Daoquan Tang,Yan Du,Shuai Ji
This paper reports a novel method for simultaneous extraction and in situ separation of flavonoids and alkaloids from mulberry leaves using a pH-responsive deep eutectic solvent (DES)/water system. Five novel DESs were synthesized, and isoquercitrin and deoxynojirimycin were respectively selected as the representative compounds of flavonoids and alkaloids to evaluate the extraction and separation efficiency. The optimal DES, namely [HexA (hexanoic acid)][MPD (2-methyl-2,4-pentanediol)], was identified, and the extraction and in situ separation conditions were optimized. After extraction and pH-induced phase separation, flavonoids were mainly enriched in the DES phase with alkaloids in the H 2 O phase, and the distribution ratios were 6.69:1 and 1:9 ([HexA][MPD]:H 2 O), respectively. Compared to traditional solvents, the [HexA][MPD]/H 2 O system showed higher extraction efficiency of flavonoids and alkaloids with higher purity. Moreover, [HexA][MPD] possessed excellent reusability. This study demonstrates the application of pH-responsive DES/H 2 O systems for simultaneous extraction and separation of natural products for the first time.
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危险品化学品经营许可证（带存储）