FOOD RESEARCH INTERNATIONAL
·
2023
·
IF 8.1
Yujiao Sun,Xushuang Yuan,Zhaojun Luo,Yungang Cao,Shuai Liu,Yang Liu
Liquid-state fermentation (LSF) of tea leaves is a promising way to obtain tea-based nutraceutical products rich in various bioactive compounds. In the study, the changes of bioactive compounds, tea pigments and complex metabolites from LSF of primary dark tea, green tea and white tea infusions with Aspergillus cristatus were determined. Chemical analyses revealed that soluble sugars, monosaccharide composition, total polyphenols, total flavonoids, free amino acids, soluble proteins and tea pigments were changed in different ways. An untargeted metabolomic analysis and ribonucleic acid sequencing (RNA-seq) based transcriptomic analysis were performed to investigate the metabolic differentiation and clarify the key differentially expressed genes (DEGs, fold change >2 and p < 0.05), showing that amino acid metabolism, carbohydrate metabolism and lipid metabolism were the most enriched pathways during A. cristatus fermentation of primary dark tea, green tea and white tea infusions. In addition, glycerophospholipid metabolism, linoleic acid metabolism and phenylalanine metabolism were greatly accumulated in the fermentation of primary dark tea and white tea infusions; Pyruvate metabolism, glycolysis/gluconeogenesis, fatty acid degradation, tyrosine metabolism, phenylalanine, tyrosine and tryptophan biosynthesis and valine and leucine, isoleucine degradation were greatly accumulated in the fermentation of primary dark tea and green tea infusions; Starch and sucrose metabolism was greatly accumulated in the fermentation of green tea and white tea infusions; Galactose metabolism was significantly enhanced in the fermentation of primary dark tea infusion; Amino sugar and nucleotide sugar metabolism, sphingolipid metabolism and alanine, aspartate and glutamate metabolism were significantly enhanced in the fermentation of green tea infusion. Besides, some other pathways involving aminobenzoate degradation, biosynthesis of cofactors, pyrimidine metabolism, benzoxazinoid biosynthesis and phenazine biosynthesis, tropane, piperidine and pyridine alkaloid biosynthesis and flavone and flavonol biosynthesis also differed from each other. These findings support that A. cristatus plays a vital role in the biochemical and genetic regulation of metabolite profile, and could be considered a potential prospect for better use of A. cristatus on different kinds of tea materials.
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DESALINATION
·
2022
·
IF 11.211
Peng Cheng,Yanling Liu,Xiaoping Wang,Kaiming Fan,Pan Li,Shengji Xia
Interlayered thin-film nanocomposite (TFN) membranes have been proven to achieve enhanced water permeance for desalination applications , while those lacking effective control over the construction of interlayers were prone to unsatisfactory permselectivity . In this study, a high-performance TFN membrane was constructed via interfacial polymerization on a Metal-Organic Frameworks (MOFs)-interlayered polyethersulfone substrate. Regulating the thickness of the MOFs (copper-tetrakis(4-carboxyphenyl) porphyrin , Cu-TCPP) interlayer could effectively tailor the structural parameters (effective filtration area, selective layer thickness, and pore size) and physicochemical properties of the membrane. A resistance-in-series model was applied to analyze the hydraulic resistance distribution and the specific value of the intrinsic solution permeability of each layer of the TFN membranes. Results showed that the interlayer with a proper deposition density of MOFs nanosheets could simultaneously increase the cross-linking degree by adsorbing the PIP monomers and reduce the thickness of the PA layer to enhance the intrinsic permeability . The optimal membrane with a thin PA film (20 nm) displayed an excellent water permeance of 32.7 L·m −2 ·h −1 ·bar −1 and an ultrahigh NaCl/Na 2 SO 4 selectivity of 271.7. Moreover, the membrane demonstrated remarkable stability during 120 h filtration. This work provides a promising strategy for fabricating TFN membranes with higher selectivity and enhanced comprehensive performance.
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APPLIED CATALYSIS A-GENERAL
·
2022
·
IF 5.723
Qin Wang,Dan Luo,Jiansu Ran,Jie Zheng,Yuntong Cui,Ruixue Yangcheng,Shuang Luo,Jianjian Wang
It is highly desirable but of challenge to develop an efficient process for lactic acid (LaA) production from xylose performed in water. Herein, a novel catalyst prepared via a facile solvent-free coordination route is developed to efficiently produce LaA from xylose in water. The structural properties of the prepared catalyst and the reaction conditions are systematically investigated. The production rate of LaA at 190 °C in water is up to 1.8 h −1 , outperforming the most recent reports (below 1.1 h −1 ). Detailed experimental and characteristic studies reveal that the presence of Lewis acidic sites and basic sites in the catalyst structure can not only lower the energy barrier of xylose conversion but also boost the efficient conversion of the active reaction intermediates into LaA. The findings of this work will provide a new approach for the efficient production of LaA from carbohydrates.
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CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Yuchao Shao,Menggang Bao,Weizhong Huo,Rong Ye,Muhammad Ajmal,Wenjing Lu
Acid hydrothermal treatment of biowaste is favorable for producing hydrochar and corresponding hydrothermal solution containing saccharides, furans and aromatic compounds, while the alkaline hydrothermal condition is beneficial for their humification to form hydrothermal humic acid (HHA). Herein, the humification potential of hydrochar, hydrothermal solution, and their mixture under alkaline hydrothermal conditions was compared to further reveal their contribution to HHA. The result showed the main source of HHA was hydrochar, occupying 70 wt% of total HHA yield from their mixture while hydrothermal solution only contributed 17 wt%. Moreover, the HHA that existed in hydrochar (denoted as HHA acid ) was 32 wt% while the hydrochar-derived HHA obtained after alkaline hydrothermal treatment (denoted as HHA alk ) increased to 65 wt%, close to that from commercial coal or lignite. The hydrochar was further separated into four components, i.e. hydrothermal fulvic acid (HFA), stable HHA acid (S-HHA acid ) non-stable HHA acid ( N -HHA acid ) and remaining substance (RS), aiming to reveal the major pathway of the incremental HHA production under alkaline hydrothermal condition. Reactions of RS + HFA, RS + N -HHA acid and self-synthesizing of RS all contributed to the incremental HHA alk yield with the major contribution (75C%) from the first combination (RS + HFA). The pathways of alkaline hydrothermal humification of hydrochar were revealed after analyzing the molecular level structure of HHA alk by UPLC-MS. This in-depth analysis fills the knowledge gap in the mechanism of hydrothermal humification of biowaste.
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BRAZILIAN JOURNAL OF MICROBIOLOGY
·
2022
·Vol. 54
·期 1
·199-211
·
IF 2.214
Chen Guoqiang,Xu Zhenxiang,Wang Fan,Liu Lijuan,Wei Yuxi,Li Jinglong,Zhang Liang,Zheng Kai,Wu Lijun,Men Xiao,Zhang Haibo
The exploration of polysaccharides from microorganisms is of great importance. In this study, a new type of exopolysaccharide excreted by Fusarium merismoides A6 (FM-EPS) was isolated, and the extraction conditions were optimized using a response surface methodology (RSM). The extraction temperature at 0 °C, a precipitation time of 7.83 h, and an ethanol precipitation concentration of 77.64% were predicted and proved to be the best extraction conditions with the maximum extraction yield of 0.74 g/mL. Then, two fractions of F . merismoides A6 exopolysaccharides (FM-EPS1 and FM-EPS2) were obtained through DEAE Sepharose fast flow column chromatography. As indicated by monosaccharide composition analysis, both fractions mainly consisted of mannose, glucose, galactose, and ribose, with an average molecular weight of 5.14 × 10 4 and 6.50 × 10 4 g/mol, respectively. FT-IR and NMR spectroscopy indicated the FM-EPSs had both α- and β-glycosidic bonds. Moreover, the determination of antioxidant and antiproliferative activities in vitro proved that FM-EPSs had good antioxidant activities and antiproliferation activities. FM-EPS1 showed stronger antioxidant activities than FM-EPS2. FM-EPS2 showed antiproliferation activities on HeLa and HepG2 cells, while FM-EPS1 had no obvious antiproliferative activity. Therefore, FM-EPSs could be explored as potential antioxidant and anticancer agent applied in food, feed, nutraceutical, pharmaceutical, cosmetics, and chemical industries.
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GREEN CHEMISTRY
·
2022
·Vol. 24
·期 22
·8812-8819
·
IF 11.034
Qiyu Liu,Liang Zhou,Xinyi Xie,Di Fan,Xinping Ouyang,Wei Fan,Xueqing Qiu
Selective production of glucose from cellulose in a molten salt hydrate (MSH) shows high efficiency but the separation of glucose is still challenging. To address this issue, we proposed a stepwise method, where cellulose was first hydrolyzed into glucan oligomers and separated via anti-solvent precipitation, followed by being hydrolyzed into glucose under mild conditions. For potential industrial applications, using raw biomass as a substrate is more attractive due to its commercial advantages. However, directly hydrolyzing raw corn stover in an MSH resulted in a low glucan oligomer yield and sugar concentration, together with insufficient separation efficiency. This work demonstrated that the generation of acetic acid during hemicellulose hydrolysis is the critical factor affecting glucan oligomer selectivity. To address this issue, a pre-extraction method using auto-hydrolysis was proposed to remove hemicellulose before MSH hydrolysis. After pre-extraction, the obtained glucan oligomer yield could be enhanced from 59.9% to 79.5%, with the separation efficiency increased from 53.7% to 80.6% by methanol precipitation. Besides, increasing the raw corn stover concentration led to an increase in acetic acid and resulted in glucan oligomer degradation. However, after removing hemicellulose, the concentration of glucan oligomers could be significantly enhanced from less than 72.1 to 121.5 mg mL−1. Thus, pre-extraction of hemicellulose followed by MSH hydrolysis provides a potential application for directly producing glucan oligomers from raw biomass with improved yield and separation efficiency.
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Polymers
·
2022
·Vol. 14
·期 21
·4636
·
IF 4.967
Zhengyu Hu,Jiaming Wang,Long Jin,Tieqiang Zong,Yuanqi Duan,Jinfeng Sun,Wei Zhou,Gao Li
This investigation focuses on the three novel polysaccharides fromCordyceps militarisand then discusses their characterization and anti-complementary activity. The three polysaccharides fromC. militaris(CMP-1, CMP-2 and CMP-3) were prepared using a DEAE-52 cellulose column. The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation. The evaluation of anti-complementary activity demonstrated that the three polysaccharides significantly inhibited complement activation through the classical pathway and alternative pathway. Preliminary mechanism studies indicated that CMP-1, CMP-2 and CMP-3 acted with C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system. The analysis of the Pearson correlation and network confirmed that the ribose, glucuronic acid and galacturonic acid composition were negatively correlated with the anti-complementary activity of polysaccharides. These results suggested that the three novel polysaccharides are potential candidates for anti-complementary drugs.Keywords:Cordyceps militaris;anti-complementary activity;polysaccharides;structure-activity relationship
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Sustainable Chemistry and Pharmacy
·
2022
·
IF 5.464
Yalan Zhang,Liang He,Qin Li,Junwen Cheng,Yanbin Wang,Jiancheng Zhao,Shaofei Yuan,Yongjian Chen,Rui Shi
To the valorization of Indocalamus tessellatus（I.tessellatus） leaves for the functional food industry, the process of ultrasonic-assisted deep eutectic solvent (DES) extraction of I.tessellatus leaves polysaccharides (U-DES-ICP) was optimized by response surface methodology . In comparison to ultrasonic extracted polysaccharides (UICP) and water extracted polysaccharides (WICP), their physicochemical properties were analyzed by HPLC , HPGPC-MALLS, and FTIR, and the structure morphology was observed by SEM . Their antioxidant activities were evaluated based on DPPH and ABTS + assay as well. The results showed that the best DES system for U-DES-ICP was a mixture of choline chloride and malonic acid in the molar ratio of 1:4 (10% water content). The highest yield of U-DES-ICP (0.6812%) was obtained by following optimized conditions: 60 min of sonication time, 17 mL/g of liquid-solid ratio, 500W of sonication power, and 63.5 °C of sonication temperature, which was significantly higher than that of the conventional methods. The structural analysis indicated that U-DES-ICP had more uronic acid with higher molecular weight compared with UICP and WICP, the relative content of uronic acid of U-DES-ICP reached 8.364%, which was twice as much as the other two polysaccharides; its molecular weight reached up to 6.201 × 10 3 KD, and the highest values of molecular weight of UICP and WICP were 1.684 × 10 3 KD and 995.4 KD, U-DES-ICP which were confirmed by SEM . The antioxidant assays reflected that U-DES-ICP possessed stronger scavenging capacities both on DPPH and ABTS + radicals with IC50 (0.48 mg/mL and 0.61 mg/mL) than the other two biomacromolecules. IC50s for DPPH and ABTS radical scavenging by UICP were 0.51 and 0.73 mg/mL, while 0.82 and 0.94 mg/mL were IC50s for both radical scavenging by WICP, respectively. Therefore, this study evidenced that a novel DES system could boost the extraction of I.tessellatus leaves polysaccharides, which produced a higher yield of product with stronger biological activities compared with traditional techniques.
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ACS Catalysis
·
2022
·
IF 13.7
Weipeng Song,Huai Liu,Junhua Zhang,Yong Sun,Lincai Peng
Furfural represents one of the most valuable biomass-derived platform molecules. Its selective production with a smart and tractable catalytic system is highly attractive for technical implementation, yet remains challenging. We herein report a promising protocol involving the mature Hβ zeolites in pure 1,4-dioxane to convert hemicellulose-related sugars into furfural with high rate and impressive selectivity under mild operating conditions. Furfural yields as high as 93.6% (from xylose) and 85.9% (from xylan) were accomplished at 140 °C in 40 min. The solvent 1,4-dioxane served as a shield to maintain the crystal structure and acidic center of Hβ as well as to inhibit the polymerization of furfural. The synergistic action of Hβ with 1,4-dioxane largely promoted the selective conversion of xylose to furfural, as the multiple immobilized acid sites of Hβ in 1,4-dioxane medium accelerated xylose-to-xylulose isomerization. The dehydration process was favorable based on its apparent activation energy of 39.1 kJ mol–1, leading to a high turnover frequency of 53.8 h–1. Density functional theory calculations revealed that the superior catalytic behavior could be ascribed to the higher adsorption of xylose than 1,4-dioxane, allowing xylose molecules to fully access the open Al sites of Hβ and the low Gibbs free energy barrier of xylose isomerization to xylulose in 1,4-dioxane over the Hβ catalyst. Moreover, the heterogeneous zeolite catalyst and the single low-boiling-point solvent 1,4-dioxane (101 °C) were readily recovered from the product with outstanding reusability. This contribution not only opens a high potential avenue for the selective production of furfural but also sheds light on the coordination mechanism of Hβ zeolite and 1,4-dioxane toward developing tailored processes.
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BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2022
·Vol. 46
·期 1
·15-23
·
IF 3.434
Ding Chenrong,Xu Chaozhong,He Tao,Liu Xu,Zhu Yafei,Sun Liqun,Ouyang Jia,Gu Xiaoli
In this work, the effect of activated carbon particles on the production of xylonic acid from xylose by Gluconobacter oxydans in a stirred tank bioreactor was investigated. The enhancement of the oxygen transfer coefficient by activated carbon particles was experimentally evaluated under different solids volume fractions, agitation and aeration rates conditions. The experimental conditions optimized by response surface methodology (agitation speed 800 rpm, aeration rate 7 L min −1 , and activated carbon 0.002%) showed a maximum oxygen transfer coefficient of 520.7 h −1 , 40.4% higher than the control runs without activated carbon particles. Under the maximum oxygen transfer coefficient condition, the xylonic acid titer reached 108.2 g/L with a volumetric productivity of 13.53 g L −1 h −1 and a specific productivity of 6.52 g/g x /h. In conclusion, the addition of activated carbon particles effectively enhanced the oxygen mass transfer rate. These results demonstrate that activated carbon particles enhanced cultivation for xylonic acid production an inexpensive and attractive alternative.
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RSC Advances
·
2022
·Vol. 12
·期 26
·16942-16954
·
IF 4.036
Yawei Zhan,Meixin Wang,Tengfei Ma,Zhiqiang Li
In this study, the effect of the γ-valerolactone (GVL)/H2O pretreatment system on bamboo (Neosinocalamus affinis) for enzymatic hydrolysis and ethanol fermentation was investigated. The performance characterization of the pretreated bamboo substrates, including the chemical composition, the structural characteristics, and the ability to produce bioethanol, were evaluated. The recovered substrates were enzymatically hydrolyzed for 48 h and then fermented to bioethanol. For the cellulose in the raw bamboo material, the highest cellulose-to-glucose conversion yield (CGCY) was achieved at 140 °C for 2 h with GVL : H2O = 8 : 2, which was 73.39%, and the cellulose-to-ethanol conversion yield (CECY) was 67.00%. This indicated that 183.5 kg of bioethanol could be produced per ton of bamboo, which was 9.71-folds higher than that directly converted from the untreated raw bamboo powder. Under these conditions, 50.60% of the active lignin can be recovered and be used as a wood-derived feedstock for further high-valued utilization. Meanwhile, the maximum concentration of fermentation inhibitors formed after pretreatment was about 140.9 mmol L−1, and had weak inhibition to the subsequent reaction. It has been shown that the cellulose could be effectively separated from bamboo and converted into bioethanol through the GVL/H2O pretreatment system.
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高影响因子
CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Xiaoyan Yin,Tingting Cai,Chao Liu,Yan Ma,Jun Hu,Jianchun Jiang,Kui Wang
Biorefinery plays a pivotal role in biomass economy with environmentally benign process. Herein, we report a coupling dihydrolevoglucosenone and aqueous p -toluenesulfonic acid (Cyrene/TsOH aq.) system for efficient saccharification of moso bamboo. More than 87.3 % of cellulose recovery could be observed under mild condition of 120 °C for 60 min, accompanied by 85.5 % hemicellulose removal and 79.4 % lignin extraction, respectively. Notably, 90.6 % of saccharified yield could be obtained including 67.7 % of oligosaccharide and 22.9 % of monosaccharide . Besides, the yielded lignin exhibited high purity of 99.1 % and outstanding uniform polydispersity index of 1.49 with nanoscale particle size around 347 nm, which is of great significance for further application. Furthermore, the synergistic effect between Cyrene and TsOH on efficiency and specificity of biorefinery was proposed by control experiments and verified by molecular dynamics simulation and quantum chemical calculation , which provided a new insight for full components biorefinery of lignocellulosic biomass.
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危险品化学品经营许可证（带存储）