INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Yunwen Zhu,Yaping Li,Xue Li,Tongfei Chen,Hepeng Zhao,Hongli Zhou
Corn silk is the stigma and style of corn and is rich in polysaccharides. Despite the extensive research on its polysaccharides, the hemostatic characteristics of effective parts and the related activities remain insufficiently explored. Corn silk polysaccharide (CSP) was extracted with hot water and purified using a diethylaminoethyl cellulose membrane. Then, it was separated with sephadex G-150 to obtain five fractions. These fractions were investigated for their potential in hemostasis, antioxidant, immune response, and anti-lung cancer activities. CSP-2, CSP-3, and CSP-4 significantly affected the coagulation indicators activated partial thromboplastin time (APTT) and thrombin time (TT) at 125–500 μg/mL. Corn silk flavonoids and saponins at 32.25 μg/mL significantly prolonged APTT, TT, and prothrombin time (PT). CSP-2, with potent antioxidant ability, approaches Vitamin C. At 25 μg/mL, CSPs nearly reached the phagocytosis of neutral red of lipopolysaccharides. The five fractions promoted the proliferation of RAW264.7 cells at 25–800 μg/mL and stimulated NO secretion at 25–100 μg/mL. CSP-2 also showed an 86 % inhibition rate effect on A549 at 200 μg/mL. These results indicate that CSP not only has hemostatic effects but also has immune and anti-lung cancer activities. Thus, it is a potential candidate compound with immune activity for managing bleeding in cancer.
研究领域:
药理学
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生物化学
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2024
·
IF 5.8
Huiling Zhong,Zhen Zhang,Mohong Wang,Yifei Fang,Ke Liu,Junqiang Yin,Jun Wu,Jianhang Du
Acute severe trauma is often associated with rapid blood loss and a high risk of infection. Based on these concerns, this study successfully constructed a multifunctional dual-layer bioactive sponge PCCT with rapid hemostatic and infection-preventing ability. Its external surface is an electrospun poly(lactic acid) (PLA) nanofiber thin film layer, which ensures its high air permeability and effectively protects against external bacterial invasion. In vitro results showed that the film is effectively resistant to invasion by typical Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria. The inner sponge layer was formed by chlorogenic acid (CGA) grafted with chitosan (CS) and loaded with tranexamic acid (TA). The abundant cationic groups on the sponge interacted with negatively charged erythrocytes and achieved rapid hemostasis at the wound site under the action of TA. In addition, the high porosity and bioactivity of the CS-CGA sponge scaffold endowed the hydrogel with good water absorption, antibacterial properties and anti-inflammatory activity, which effectively accelerated the healing of acute infected wounds in rats and demonstrated favorable biosafety.
研究领域:
生物材料学
·
创伤修复
·
药剂学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yuhan Mai,Yang Zhou,Qihuang Ye,Zhiyou Wang,Chao Li,Yanling Chen,Huipingi Xiao,Yanyu Hu,Jiahui Li,Wei Zhang,Tongyao Wang,Haili Lin,Longguang Jiang,Cai Yuan,Peng Xu,Mingdong Huang
Microbial infection in open wounds can delay healing and lead to severe complications. This study introduces a bacterial-responsive cellulose-based fabric wound dressing (Fabric-PAC) with integrated antimicrobial and hemostatic functions. Fabric-PAC is chemically modified with polyphosphate (PolyP) and low-dose silver nanoparticles (AgNPs), and further functionalized with a phthalocyanine-based photosensitizer via supramolecular interactions on surface. Upon contact with bacteria, the photosensitizer is displaced, exposing PolyP to initiate blood coagulation and platelet-deriven tissue remodeling, promoting wound healing. Simultaneously, AgNPs are also exposed to provede sustained bacteriostasis. Moreover, the positively charged photosensitizer tightly attaches on the bacterial surface, and triggers the antimicrobial photodynamic effect upon illumination, rapidly eliminating pathogens. This study not only introduces a bacteria-responsive wound dressing with synergistic antimicrobial and hemostatic effects, but also offers a new strategy for the functionalization of cellulose-based materials.
研究领域:
生物医学工程
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材料科学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Ying Wang,Hanlu Chen,Xiaoli Liu,Jingxuan Song,Senxin Xu,Reziwanguli Abulaiti,Dongmei Han,Yang Hu,Zhihua Wang,Fubo Gu
Hermetia illucens , with a short growth cycle, is promising as a valuable source of chitin. However, the optimal method for extracting chitin from this insect and its application for hemostasis has not been addressed. This work employed an environmentally friendly choline chloride-lactic acid deep eutectic solvent technology to extract chitin effectively from the Hermetia illucens pupae shells, realizing one-step removal of inorganic salts and proteins. The extraction process was optimized through the response surface methodology, which identified the optimal temperature (92 °C), time (5.6 h), and liquid-solid ratio (20 mL/g) as the conditions that yielded the highest chitin yield (28.40 %) and the highest purity (96.93 %). The structural characterization of the extracted chitin revealed an α-type structure with favorable thermal stability. Furthermore, by regulating the degree of deacetylation, we optimized the degree of protonation of chitin for producing a series of chitosan (Cts), thereby enabling the production of Cts2 with excellent in vitro hemostatic properties. The superior hemostatic performance of Cts2 can be attributed to significantly superior platelet adhesion, achieved preferred adsorption of fibrinogen, and improved blood coagulation. This work provides a theoretical basis for the efficient extraction and high-value application of chitin from Hermetia illucens .
研究领域:
生物化学
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材料科学
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医学
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DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Xiaohui Wei,Sheng Ding,Shisheng Liu,Kun Yang,Junjie Cai,Fan Li,Chunlai Wang,Song Lin,Feng Tian
Serial hemostatic sponges consisting of polysaccharides-modified chitosan foam sponges were prepared by Schiff base crosslinking reaction between the deacetylated chitosan and oxidized dialdehyde cellulose. Such composite foam sponges were characterized by scanning electron microscopy and Fourier-transform infrared spectroscopy to confirm their morphology and compositions. Then the coagulation process was evaluated in vitro by thrombus elasticity meters. Furthermore, the hemostasis experiments on mouse tail vein and rabbit femoral artery were also performed in vivo . The results strongly indicated that such synergistic cellulose-modified chitosan foam sponges showed comprehensively excellent water-absorbing quality, improved mechanical performance, low hemolysis rates, benign cytotoxicity, good resilience ability after repeated compression, and superior hemostasis capability both in vitro and in vivo . Furthermore, the hemostatic mechanism is via adhering/activating the red blood cell/platelet to form robust blood clots through the endogenous coagulation pathway, which serves as a good candidate for emergency trauma treatment in daily civilian and military hemostasis.
研究领域:
生物材料学
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医学工程
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血液学
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产品关联
DOI
PubMed
高影响因子
Materials Advances
·
2021
·Vol. 2
·期 15
·5150-5159
·
IF 0
Xingxia Zhang,Zhifang Ma,Yue Ke,Yu Xia,Xiaodong Xu,Jingchuan Liu,Yumei Gong,Qiang Shi,Jinghua Yin
Biocompatibility, hemostatic performance and wound healing capability are key limitations for the currently available hemostatic agents. To overcome these problems, a hydrogel inspired by a platelet coagulation mediator is developed in this work as a new class of hemostatic adhesive with improved performance and wound healing capability. The hydrogel is prepared using highly biocompatible serotonin and chondroitin sulfate (CS), both of which are natural components of the body. The structural, physical and biological and hemostatic properties of the hydrogel are characterized in detail. It is demonstrated that serotonin acts as a crosslinker to form adhesive hydrogels and as a blood clotting mediator for rapid hemostasis. Chondroitin sulfate regulates cell behaviors and fates to facilitate wound healing. The serotonin-conjugated chondroitin sulfate hydrogel exhibits improved hemostatic capability in vivo and rapid wound healing after hemostasis. In addition, the wound healing capability of the hydrogel is further improved with the aloe vera powder, confirming the versatility of the hydrogel system. Therefore, chondroitin sulfate–serotonin hydrogels exhibit the potential for effective hemostasis and wound healing.
研究领域:
生物材料
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医学工程
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再生医学
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产品关联
DOI
ACS Applied Materials & Interfaces
·
2017
·
IF 7.504
Dedai Lu,Hongsen Wang,Ting’e Li,Yunfei Li,Fajuan Dou,Shaobo Sun,Hongyun Guo,Shiqi Liao,Zhiwang Yang,Qiangbing Wei,Ziqiang Lei
Inspired by marine mussel adhesive proteins, polymers with catechol side groups have been extensively explored in industrial and academic research. Here, Pluronic L-31 alcoholate ions were used as the initiator to prepare a series of polypeptide–Pluronic–polypeptide triblock copolymers via ring-opening polymerization of l-DOPA-N-carboxyanhydride (DOPA-NCA), l-arginine-NCA (Arg-NCA), l-cysteine-NCA (Cys-NCA), and ε-N-acryloyl lysine-NCA (Ac-Lys-NCA). These copolymers demonstrated good biodegradability, biocompatibility, and thermoresponsive properties. Adhesion tests using porcine skin and bone as adherends demonstrated lap-shear adhesion strengths up to 106 kPa and tensile adhesion strengths up to 675 kPa. The antibleeding activity and tissue adhesive ability were evaluated using a rat model. These polypeptide–Pluronic copolymer glues showed superior hemostatic properties and superior effects in wound healing and osteotomy gaps. Complete healing of skin incisions and remodeling of osteotomy gaps were observed in all rats after 14 and 60 days, respectively. These copolymers have potential uses as tissue adhesives, antibleeding, and tissue engineering materials.
研究领域:
生物材料
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医学
·
高分子化学
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产品关联
DOI
PubMed
高影响因子
Chemistry Central Journal
·
2017
·Vol. 11
·期 1
·1-15
·
IF 2.442
Zhang Xiaoyan,Sun Wenbo,Yang Zhou,Liang Yan,Zhou Wei,Tang Lei
Background
Toddalia asiatica root bark as an effective hemostatic natural medicine or Chinese materia medica was applied in China for long history, its complex drug action mechanisms and unclear substance basis have been constraining the development of this drug.Results An intelligentized strategy by LC-ESI Q-TOF MSE was presented in this study for rapid identification of hemostatic chemical constituents from this natural medicine. Chromatographic separation was performed on a C18 column (150 mm × 2.1 mm, 1.8 μm), the MSE data in both negative and positive ion modes were acquired to record the high-accuracy MS and MS/MS data of all precursor ions. To reduce the false positive identifications, structural confirmation was conducted by comparison with the isolated reference standards (tR and MS, MS/MS data) or matching with natural product databases. Bioassay-guided fractionation of the extract of T. asiatica root bark was also carried out.Conclusion sAs a consequence, 31 natural compounds in T. asiatica root bark got putatively characterized. There were four main coumarins, isopimpinellin (Cp.23), pimpinellin (Cp.24), coumurrayin (Cp.30) and phellopterin (Cp.34) isolated and identified from T. asiatica root bark. The present study provided candidate strategy that helps to effectively identify the primary natural compounds of TCM or other complex natural medicines, and then promote development and application of natural medicines and their medicinal resources.
研究领域:
药理学
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中药学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2015
·
IF 2.858
Jing-jing Rong,Ming Liang,Feng-qi Xuan,Jing-yang Sun,Li-jun Zhao,Hui-zhen Zhen,Xiao-xiang Tian,Dan Liu,Quan-yu Zhang,Cheng-fei Peng,Tian-ming Yao,Fei Li,Xiao-zeng Wang,Ya-ling Han,Wei-ting Yu
To date, transcatheter arterial embolization (TAE) has become a standard treatment to control intracavitary bleeding as an alternative to surgery. Due to excellent biocompatibility and no residual in vivo , biodegradable materials are preferred in TAE. However, gelfoam is the only commercially available biodegradable embolic material used to treat blunt trauma of solid abdominal viscera until now, and controversial on its stability and reliability never stopped in the past five decades. In this study, a new biodegradable macromolecule material (thrombin-loaded alginate-calcium microspheres, TACMs) was prepared using electrostatic droplet techniques and a special method was developed for hemostatic embolization. Thrombin was successfully loaded into microspheres with high encapsulation efficiency and drug loading capacity. A burst release of TACMs was observed at early stage and sustained release later on, with the activity of thrombin preserved well. The strength of TACMs mixed thrombus, which was used as embolic agent, increased in a dose-dependent manner after TACMs were added. In addition, the TACMs were verified to be of no cytotoxicity and systemic toxicity, and biodegradable in vivo . Finally, the results of preliminary applications revealed that the TACMs could serve as an effective and promising embolic material for blunt trauma and hemorrhage of solid abdominal viscera.
研究领域:
医学
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生物材料学
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产品关联
DOI
PubMed
高影响因子
Micromachines
·
2018
·Vol. 9
·期 10
·501
·
IF 2.222
Shuren Song,Da Chen,Hongfei Wang,Chaohui Li,Wei Wang,Wangli Yu,Yanyan Wang,Qiuquan Guo
Measurement of hemostatic parameters is essential for patients receiving long-term oral anticoagulant agents. In this paper, we present a shear mode bulk acoustic resonator based on an inclinedc-axis aluminum nitride (AlN) film for monitoring the human hemostatic parameters. During the blood coagulation process, the resonant frequency of the device decreases along with a step-ladder profile due to the viscosity change during the formation of fibers in blood, revealing the sequential coagulation stages. Two hemostatic parameters with clinical significance, prothrombin time (PT) along with its derived measure of international normalized ratio (INR), are determined from time-frequency curves of the device. Furthermore, the resonator is compared with a commercial coagulometer by monitoring the hemostatic parameters for one month in a patient taking the oral anticoagulant. The results are consistent. In addition, thanks to the excellent potential for integration, miniaturization and the availability of direct digital signals, the proposed device has promising application for point of care coagulation monitoring.Keywords:bulk acoustic wave;AlN film;hemostatic parameters;viscosity sensor
研究领域:
生物医学工程
·
临床检验学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 6
·915-926
·
IF 7.571
Liwei Zhang,Yajie Zhang,Fanshu Ma,Xingzhu Liu,Yangzhong Liu,Yi Cao,Renjun Pei
Hydrogel-based wound dressings with tissue adhesion abilities are widely used for wound closure. However, currently developed hydrogel adhesives are still poor at continuing to seal wounds while bleeding is ongoing. Herein, we demonstrate an antibacterial and hemostatic hydrogel adhesive with low-swelling properties and toughness for wound healing. The hydrogel was composed of Pluronic F127 diacrylate, quaternized chitosan diacrylate, silk fibroin, and tannic acid, and it was not only able to maintain good tissue adhesion abilities in a moist environment but it also showed guaranteed tissue adhesion and mechanical strength after absorbing water due to its low-swelling and toughness properties. Furthermore, in vitro and in vivo tests demonstrated that the hydrogel also had antibacterial, antioxidant, and hemostatic properties, which could promote tissue regeneration. All these findings demonstrate that this hydrogel with multifunctional properties is a promising material for clinical wound healing applications.
研究领域:
生物医学工程
·
材料科学
·
组织工程
✓ 已验证
DOI
PubMed
高影响因子
Biomaterials Science
·
2021
·Vol. 9
·期 18
·6098-6107
·
IF 6.843
Yuting Shi,Xiaoquan Ding,Yufei Cao,Haicun Zhou,Wenwen Yu,Mingsheng Liu,Juanjuan Yin,Hongbin Liu,Jingjing Wang,Congshu Huang,Chenliang Gong,Hua Wei,Guanghui Zhao
Thrombin is a serine protease known as activated coagulation factor II and is primarily applied as an effective local hemostatic agent. However, its clinical application is hindered by drawbacks, such as high sensitivity to the surrounding environment, instability and poor storage stability, easy inactivation, and low bioavailability. The biological functions of biomacromolecules in harsh environments can be preserved through biomineralization. Despite the success of biomimetic mineralization, limited consideration has been given to the mineral-based methods and the effect of various metal ions on enzyme activity. To explore an efficient technique for biomimetic mineralized thrombin, six kinds of ion/thrombin hybrid microflowers and two kinds of thrombin/MOF were synthesized in this work. The results showed that Zn-HNFs-G exhibits good hemostatic effect and maintains high enzymatic activity when exposed to high-temperature conditions. Meanwhile, Fe-HNFs-G, Thrombin@ZIF-8-G and Thrombin@MAF-7-G possess negligible enzyme protection.
研究领域:
生物医学工程
·
材料科学
·
止血
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）