Polymers
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2022
·Vol. 14
·期 10
·1997
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IF 4.967
Tong Chen,Sanping Zhao
An effective strategy was developed to fabricate novel lanthanide ions–pyromellitic acid–methoxy poly(ethylene glycol) (Ln-PMA-MPEG) nano-assemblies. The amphiphilic partially esterified derivative (PMA-MPEG) of pyromellitic acid with methoxy poly(ethylene glycol) was designed and synthesized via the coupling reaction. Ln-PMA-MPEG nano-assemblies were rapidly fabricated using PMA-MPEG as a polymer ligand with Eu3+ions or mixed Eu3+/Tb3+ions through biomimetic mineralization in neutral aqueous systems. The size of the as-prepared materials could be designed in the range 80–200 nm with a uniform distribution. The materials were readily dispersed in various solvents and displayed visible color variations and different photoluminescent properties for solvent recognition. The mixed Eu/Tb-PMA-MPEG nanomaterials were investigated as ratiometric sensors for the detection of trace water in DMF and Fe3+ions in aqueous solutions. The sensor materials can quantitatively detect trace water in DMF from 0% to 10% (v/v). The resultant materials also display a strong correlation between the double luminescence intensity ratios (ITb/IEu) and Fe3+concentration, with a good linear detection concentration in the range of 0–0.24 mM and a limit of detection of 0.46 μM, and other metal ions did not interfere with the sensing mechanism for Fe3+ions. The novel nano-assemblies have potential applications as ratiometric fluorescent nanosensors in the chemical industry as well as in biomedical fields.Keywords:metal–organic frameworks;biomimetic mineralization;luminescent properties;fluorescent nanosensors
研究领域:
材料科学
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生物医学工程
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化学
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DOI
PubMed
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 5
·532-540
·
IF 3.532
Hua-Rui Nan,Yun-Hai Liu,Wen-Juan Gong,Hong-Bo Peng,You-Qun Wang,Zhi-Bin Zhang,Xiao-Hong Cao
In this work, a ratiometric fluorescence system was designed for the detection of trace UO22+ in water based on the inner filter effect (IFE) between gold nanoparticles (AuNPs) and gold nanoclusters (AuNCs). IFE-induced fluorescence quenching was achieved due to the enhanced complementary overlap between the absorption spectra of AuNPs and the emission spectrum of AuNCs after the addition of UO22+. Blue carbon dots (B-CDs) were added to serve as reference fluorophores to expand the color tonality and make human eye recognition easier. The ratiometric fluorescent sensor demonstrated a unique fluorescence color change from red to blue when different doses of UO22+ were added, with a detection limit of 8.4 nM. Furthermore, the ratiometric fluorescent sensor was effectively used for UO22+ determination in real-world water samples, with acceptable recoveries.
研究领域:
分析化学
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环境科学
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DOI
PubMed
高影响因子
Nanoscale
·
2023
·
IF 6.7
Jiawen Han,Yuwei Zhang,Xujuan Lv,Daoqing Fan,Shaojun Dong
As a kind of well-known disease biomarker, uric acid (UA) is closely associated with normal metabolism and health. Despite versatile nanozymes facilitating the analysis of UA, most previous works could only generate single-signal outputs with unsatisfactory detection performance. Exploring a novel ratiometric fluorescent UA sensor with high sensitivity, reliability and portable sensing ability based on facile, low-cost nanozymes is still challenging. Herein, we report the first metal–organic-framework (MOF) nanozyme-originated ratiometric fluorescent UA sensor based on Fe3Ni-MOF-NH2 propelled UA/uricase/o-phenylenediamine tandem catalytic reaction. Different from previous reports, the peroxidase-like property and fluorescence of Fe3Ni-MOF-NH2 were simultaneously employed. In the absence of UA, only the MOF's fluorescence at 430 nm (FI430) can be observed, while the addition of UA will initiate UA/uricase catalytic reaction, and the generated H2O2 could oxidize o-phenylenediamine into highly fluorescent 2,3-diaminophenazine (DAP) (emission at 565 nm, FI565) under the catalysis of the MOF nanozyme. Coincidently, MOF's fluorescence can be quenched by DAP via the inner filter effect, resulting in a low FI430 value and high FI565 value, respectively. Therefore, H2O2 and UA can be alternatively detected through monitoring the above contrary fluorescence changes. The limit of detection for UA is 24 nM, which is much lower than those in most previous works, and the lowest among nanozyme-based ratiometric fluorescent UA sensors reported to date. Moreover, the portable sensing of UA via smartphone-based RGB analysis was facilely achieved by virtue of the above nanozyme-propelled tandem catalytic system, and MOF nanozyme-based molecular contrary logic pairs were further implemented accordingly.
研究领域:
生物化学
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医学诊断
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材料科学
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DOI
PubMed
高影响因子
POLYMER INTERNATIONAL
·
2023
·
IF 3.2
Wenming Yang,Mingyue Kuai,Changchun Wu,Wenqi Yang,Lingling Cao,Yi Zhang,Ting Chen,Yunfei Cao,Baozhi Wang,Wanzhen Xu
In this work, a dual-emission ratiometric fluorescence sensor was prepared and used for the detection of sulfadiazine (SDZ) drug. The hydrothermal method was used to synthesize carbon quantum dots (CQD) (fluorescence response signal) and sulfhydryl-modified cadmium telluride quantum dots (fluorescence reference signal), and a molecularly imprinted polymer was subsequently synthesized. After eluting the template molecule SDZ, the blot recognition site remains on the surface of the imprinted polymer. During the process of detection, due to electron transfer between SDZ and CQDs, holes of stimulated electrons are filled. This leads to fluorescence quenching at around 430 nm, which greatly improves sensor selection performance. Compared with traditional single-emission fluorescence sensors, ratiometric fluorescence sensors have a wider linear range and higher detection accuracy. Under the optimal conditions, the sensor exhibited excellent performance in terms of low detection limit (4.2 nmol L −1 ) and satisfactory linear range (2–20 μmol L −1 ). This intelligent response change mode also provides prospect for detection of other sulfonamide antibiotics and offers a reference to signal transform for other molecular imprinting technologies. © 2023 Society of Industrial Chemistry.
研究领域:
分析化学
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药物分析
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材料科学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Xia Wu,Xi Zhang,Jianbo Ma,Yunyi Zhang,Ming Li
In this study, a smart phone assisted ratiometric fluorescence sensor was designed for detecting dopamine (DA). The ratiometric fluorescence sensor was prepared by simple physical mixing green quantum dots (GQDS) and red quantum dots (RQDS). DA could induce gold nanoparticles (AuNPs) aggregate via hydrogen-bonding interactions, and further changed the absorption spectrum of gold nanoparticles to overlap with a certain emission spectrum of ratiometric fluorescence sensor. AuNPs had inner filtration effect (IFE) on the ratiometric fluorescence sensor. Due to the IFE, the dispersive AuNPs could quench GQDS, whereas the clustered AuNPs could quench RQDS. With the addition of DA, the color of ratiometric fluorescence changed from orange red to green. To simplify the detection process, a smartphone was employed to detecting DA in human urine by measuring RGB value of fluorescence color changes with a detection limit of 86 nM. This proposed method has the advantages of low cost, easy prevalence and simple operation, thus provides a great promise for rapid detection of biomarker in biological samples.
研究领域:
分析化学
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生物医学工程
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纳米技术
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DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Nan Jiang,Peng Li,Shihao Sun,Wei Wei
Since 5-hydroxymethylfurfural (HMF) becomes a neo-forming contaminant with latent harm to human health, development of new method for highly sensitive detection of HMF is extremely desirable. Herein, a novel ratiometric fluorescence sensor based on strand displacement reaction and magnetic separation was designed for sensitive and selective detection of HMF with the help of MnO 2 nanosheets . The aldehyde-functionalized DNA (S0–CHO) and HMF competed for binding to amino-functionalized magnetic beads (NH 2 -MBs). Then, S0–CHO was collected from supernatant by magnetic separation. In the presence of HMF, the obtained S0–CHO induced the formation of T-shaped DNA by strand displacement reaction (SDR), lighting the fluorescence of FAM. In the absence of HMF, no S0–CHO was present to ignite T-shaped DNA. In this situation, fluorescence of Cy5 was turned on. Thus, a ratiometric fluorescence sensor for high-sensitive detection of HMF was developed. The sensor has a wide linear range from 5 nM to 5 μM. It also exhibited high selectivity against other potential interfering substances. It has been successfully applied to analyze HMF in food samples. The method has potential to be expanded to detect other molecules containing aldehyde groups and further be applied in food safety, environment and other fields.
研究领域:
生物化学
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分析化学
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食品安全
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DOI
高影响因子
Nanoscale Research Letters
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2018
·Vol. 13
·期 1
·1-9
·
IF 3.125
Liu Mingyue,Gao Zhao,Yu Yanjun,Su Rongxin,Huang Renliang,Qi Wei,He Zhimin
4-Nitrophenol (4-NP) is a priority pollutant in water and is both carcinogenic and genotoxic to humans and wildlife even at very low concentrations. Thus, we herein fabricated a novel molecularly imprinted core-shell nanohybrid as a ratiometric fluorescent sensor for the highly sensitive and selective detection of 4-NP. This sensor was functioned by the transfer of fluorescence resonance energy between photoluminescent carbon dots (CDs) and 4-NP. This sensor was synthesized by linking organosilane-functionalized CDs to silica-coated CdSe quantum dots (CdSe@SiO 2 ) via Si–O bonds. The nanohybrids were further modified by anchoring a molecularly imprinted polymer (MIP) layer on the ratiometric fluorescent sensor through a facile sol–gel polymerization method. The morphology, chemical structure, and optical properties of the resulting molecularly imprinted dual-emission fluorescent probe were characterized by transmission electron microscopy and spectroscopic analysis. The probe was then applied in the detection of 4-NP and exhibited good linearity between 0.051 and 13.7 μg/mL, in addition to a low detection limit of 0.026 μg/mL. Furthermore, the simplicity, reliability, high selectivity, and high sensitivity of the developed sensor demonstrate that the combination of MIPs and ratiometric fluorescence allows the preparation of excellent fluorescent sensors for the detection of trace or ultra-trace analytes.
研究领域:
环境科学
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分析化学
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材料科学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2025
·
IF 4.6
Xia Lu,Qinyi Gao,Liqing Xu,Yanxin Ren,Fenfen Tong,Yuxin Wang,Linfan Wang,Na Ge,Wupeng Ge
This study successfully synthesized high performance blue emissive carbon dots (ZrN-CDs) using citric acid, zirconium chloride, and ethylenediamine as precursors through a hydrothermal method. The resulting ZrN-CDs exhibit excitation-independent emission characteristics, along with good water solubility, salt resistance, and photobleaching resistance. The fluorescence resonance energy transfer (FRET) mechanisms allow for the selective attenuation of ZrN-CD fluorescence by tetracyclines (TCs). Building on this effect, a dual-emission ratiometric fluorescent sensor was developed by integrating europium ions (Eu 3+ ), featuring an inverse fluorescence signal response to enhance detection accuracy through self-calibration. The fluorescence emission behavior of ZrN-CDs (F 440 ) showed selective quenching in response to TCs, while the characteristic emission of Eu 3+ (F 615 ) exhibited fluorescence enhancement due to the antenna effect (AE). Quantification of common TCs antibiotics, including tetracycline (TET) and oxytetracycline (OTC), was achieved by recording the fluorescence intensity ratio (F 615 /F 440 ). The limits of detection (LOD) for TET and OTC reached 0.029 μM and 0.016 μM, respectively. This method was successfully applied to the detection of TET in bovine, goat, and camel milk, with recovery rates ranging from 83.65% to 106.60%, thereby confirming the reliability and effectiveness of the sensor in practical applications.
研究领域:
分析化学
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环境科学
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食品科学
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Xin Yuan Li,Qing Hong Long,Zhoujian Pan,Xiao Han Ma,Chunhua Xia,Xi Mai,Na Li
Developing rapid tetracycline sensing system is of great significance to monitor the illegal addition to drugs and pollution to food and ecosystem. By loading covalent organic frameworks (COFs) with Eu 3+ , a new hybridized material (COF@Eu 3+ ) was prepared for tetracycline determination. Based on the Schiff base reaction, the COFs were by synthesized through solvent evaporation in 30 min at room temperature. Thereafter, Eu 3+ was modified into COFs to develop the COF@Eu 3+ sensing platform by adsorption and coordination. In presence of tetracycline, tetracycline can displace water molecules and coordinate with Eu 3+ through the antenna effect. As a result, the red fluorescence of Eu 3+ was enhanced by tetracycline with green fluorescence of COF as a reference. The developed ratiometric fluorescence sensor exhibits a linear range of 0.1–20 μM for detecting tetracycline with a detection limit of 30 nM. Integrated with a smartphone, the rapid tetracycline detection can be realized in situ, which is potential for high-throughput screening of tetracycline contaminated samples. Furthermore, the COF@Eu 3+ fluorescence sensor has been successfully applied to the detection of tetracycline in traditional Chinese medicine compound preparation with satisfied recoveries. Therefore, a smartphone-assisted device was successfully developed based on Eu 3+ -functionalized COF, which is an attractive candidate for further applications of fluorescence sensing and visual detection.
研究领域:
分析化学
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环境科学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Jiao Wang,Ziying Zhang,Xia Gao,Xiaodong Lin,Yaqing Liu,Shuo Wang
Lead ion (Pb 2+ ) is known as a major non-degradable environmental pollutant and is quite harmful to human health. In our investigation, a novel ratiometric fluorescent nanosensor with DNA-AgNCs as single fluorophore has been successfully constructed for ultrasensitive and specific detection of Pb 2+ . The single-strand DNA templated silver nanoclusters present green emission, which can be converted into red emission after approaching a specific DNA segment by forming duplex with its complementary DNA strands (ds-DNA-AgNCs). The ds-DNA-AgNCs contains rA cleavage site of Pb 2+ -dependent DNAzyme configuration. In the presence of Pb 2+ , the specific DNA segment would be released from the ds-DNA-AgNCs, resulting in discoloration of DNA-AgNCs from red to green. Since Pb 2+ can successively cleave the rA site, the signal change from red to green emission is amplified. Thus, benefiting from the Pb 2+ -dependent DNAzyme and the charming properties of DNA-AgNCs, the proposed platform exhibits a good linear relationship from 0.001 nM to 10 nM with a detection limit of 1.0 pM for Pb 2+ determination, which is lower than most of the reported Pb 2+ biosensors. Moreover, this sensitive ratiometric fluorescent probe exhibits excellent selectivity toward Pb 2+ detection in real samples, such as lake water, tap water, and human serum samples, illustrating the huge potential applications for complicated samples in the future.
研究领域:
生物化学
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环境科学
·
分析化学
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产品关联
DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Yue Du,Yonghai Song,Juan Hao,Keying Cai,Nan Liu,Li Yang,Li Wang
A novel ratiometric fluorescent sensor for superoxide radical (O 2 •− ) detection was developed based on Schiff base polymer (SBP)/rhodamine-B (SBP/RDB) nanocomposites. The SBP/RDB was investigated by atomic force microscopy , scanning electron microscopy, transmission electron microscopy , X-ray powder diffraction, Fourier transform infrared spectroscopy, N 2 adsorption/desorption isotherms and fluorescence spectroscopy, etc. The results showed the SBP was two-dimensional (2D) crystalline nanosheet with the thickness of 0.5 nm. RDB could be self-assembled on each of 2D SBP surface to form SBP/RDB due to the hydrogen bond between N atoms of SBP and –COOH of RDB as well as π-π interaction. The SBP/RDB exhibited the characteristic fluorescence emission of both SBP at 485 nm and RDB at 585 nm under an excitation of 270 nm. The 2D SBP was not only acted as template to assemble RDB, but also used as reference fluorescence for O 2 •− detection. The SBP/RDB fluorescence probe for O 2 •− detection demonstrated high sensitivity and selectivity with good linear range (11.1 ± 0.76 nM–8.0 ± 0.36 μM) and low detection limit (3.7 ± 0.42 nM). Because of the low cost and simple operation, the work sheds some new light to construct ratiometric fluorescent sensors based on SBP to detect O 2 •− in drinking water and human blood serum for commercial applications.
研究领域:
生物化学
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材料科学
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分析化学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Long Yu,Qutong Zheng,Di Wu,Yuxiu Xiao
pH is of great importance in understanding physiological and pathologic processes. Abnormal gastric juice pH is an indicator of many gastric diseases such as peptic ulcer and gastric cancer. In this work, a nanocomposite 1-hydroxypyrene@ Co/Tb- dipicolinic acid metal-organic framework (1-OHP@Co/Tb-DPA MOF) was constructed as a ratiometric fluorescent pH sensor for strong acidity. The nanocomposite-based pH sensor was response-rapid (30 s), linearly responsive in the broad pH range of 0.3–7.8 ( r > 0.95) and ultra-sensitive in the pH range of 0.3–5.0. Importantly, the nanocomposite-loaded fiber paper was prepared as a visual microsensor for point-of-care pH detection via ratiometric chromaticity, coupled with a homemade portable determination device. The paper-based visual microsensor is response-rapid and sensitive, long-term stable (at least 30 days), and suitable for pH assay of microvolume (as little as 5 μL) samples at pH 0.3–5.0. Both the nanocomposite-based ratiometric fluorescent sensor and the paper-based visual microsensor can accurately determine the pH of artificial gastric juices (relative error <5.4%). To our knowledge, this is the first time to report a MOF-based ratiometric fluorescent pH sensor for strong acidity (pH<1) and a paper-based pH microsensor for point-of-care visual assay via ratiometric chromaticity.
研究领域:
生物医学工程
·
分析化学
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产品关联
DOI
高影响因子
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