JOURNAL OF CHROMATOGRAPHY A
·
2016
·
IF 3.926
Yanhao Yuan,Yan Liu,Weidi Teng,Jiean Tan,Yong Liang,Youwen Tang
In the current study, a new strategy for the extraction of bisphenol A (BPA) from milk has been employed by using surface-imprinted core-shell magnetic beads, prepared by the reversible addition-fragmentation chain transfer (RAFT) polymerization. In order to obtain highly selective recognition cavities, an enhanced imprinting method based on binary functional monomers , e.g. 4-vinylpyridine (4-VP) and β-cyclodextrin (β-CD), was chosen for BPA imprinting. The morphological and magnetic properties of the Fe 3 O 4 -MIP beads were characterized by Transmission Electron Microscopy (TEM), Fourier Transform Infrared (FT-IR) Spectroscopy, Thermogravimetric Analysis (TGA), and Vibrating Sample Magnetometer (VSM). The characterization results suggested that MIP was synthesized evenly on Fe 3 O 4 -SiO 2 surface. The adsorption experiments revealed that Fe 3 O 4 -MIPs showed better extraction capacity and selectivity toward BPA and its analogues than the non-imprinted polymers (NIPs). The saturation capacity of Fe 3 O 4 -MIP was 17.98 mg/g. In milk samples, the present method displayed a lower the detection thresholds, down to 3.7 μg/L. The recoveries of BPA in milk samples for three concentrations were found to be within 99.21%, 98.07% and 97.23%, respectively to three concentrations: 1.0 μmol/L, 10.0 mol/L, 100.0 μmol/L. Thus, the MIPs can be used for remove BPA in milk samples.
研究领域:
分析化学
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环境科学
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材料科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
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2020
·Vol. 73
·期 3
·552-562
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IF 1.636
Liang Wang,Enyan Gao,Man Hu,Qiang He,Yuting He,Xiaoyan Zheng
Milk beer is a new type of dairy beverage in China and increasingly popular with consumers. The strain FXJ1 isolated in our laboratory was an excellent strain for the production of milk beer with alcohol content of 0.48%. Our research verified that the strain FXJ1 was superior to the commercial strain MJ for milk beer production and got the optimal medium formula (67.37 g/L sucrose, 29.7 g/L yeast extract, 15.61 g/L corn steep liquor, 4.13 g/L KH 2 PO 4 and 0.3 g/L MgSO 4 ) which allowed the biomass production of strain FXJ1 to achieve 8.88 ± 0.08 g/L and increase 2.94-fold as compared with yeast extract peptone dextrose base medium.
研究领域:
食品科学
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微生物学
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DOI
高影响因子
APPLIED SPECTROSCOPY
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2021
·
IF 2.388
Zichen Yang,Chaoqun Ma,Jiao Gu,Yamin Wu,Chun Zhu,Lei Li,Hui Gao,Ye Zhang,Yunpeng Shang,Chengwei Wang,Guoqing Chen
Tetracycline, an animal antibiotic, may remain in milk to cause harm to human health. For economic reasons, the abuse of antibiotics is becoming more and more common. Therefore, the abuse of tetracycline has alarmed the dairy industry and many countries such as New Zealand, China, and the USA have proposed strict standards. Surface-enhanced Raman scattering (SERS) is an emerging detection method which has been applied in food detection with the advantages of no complex pretreatment, fast detection, and weak water environment interference. Considering the abuse of antibiotics in dairy industry, we used polydimethylsiloxane (PDMS) plasma cavity as SERS substrate to detect tetracycline in milk. We found that the enhancement ability of PDMS substrate is affected by addition of 4-amino-1-butanol and complex interplay in the milk--tetracycline system. The modified PDMS plasma cavity has high SERS sensitivity that allows us to achieve low detection limit of 0.28 μg/L. The correlation coefficient was 0.987. The detection of tetracycline in milk using PDMS substrate is quick (within 10 min) and it provides a possible method for in-site detection of tetracycline.
研究领域:
食品安全
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分析化学
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光谱学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2016
·
IF 3.445
Faizan A. Sadiq,Yun Li,TongJie Liu,Steve Flint,Guohua Zhang,Lei Yuan,Zhipeng Pei,GuoQing He
The propensity for aerobic bacilli and allied genera to produce highly heat-resistant spores and thermally stable spoilage enzymes are major bacteriological issues faced by the dairy industry. Most of the enzymes are able to survive any heat treatment applied during the manufacture of milk powders and have the potential to remain active in milk powders and other dairy products during storage, and may explain some of the sensory and functionality defects reported in dairy products . Despite many reports on the occurrence of spore-forming bacteria in dairy products, knowledge about food quality related properties of many aerobic sporeformers is still scarce. Therefore, the aim of this study was to determine thermal resistance and spoilage potential of a large pool of mesophilic and thermophilic sporeformers, representing 738 isolates and 31 different RAPD groups, recently isolated from Chinese milk powders. Spore formers producing highly heat resistant spores (surviving 125 °C for 30 min) included 2 thermophiles ( Geobacillus thermoleovorans group and Geobacillus stearothermophilus ) and one mesophilic species ( Brevibacillus brevis ). Paenibacillus macerans showed the highest proteolytic activity followed by members of the Bacillus cereus group, Br. brevis , Bacillus subtilis , G. thermoleovorans group and Virgibacillus proomii . The highest lipase producing strains belonged to Bacillus licheniformis . Phospholipase activity was only shown by members of the B. cereus group and Brevibacillus parabrevis . Ten strains showed positive β -galactosidase activity, while, 4 strains showed positive haemolytic activity. B. licheniformis strains, despite belonging to one RAPD group or sub-group showed markedly different phenotypic characters which support the previous findings of heterogeneity in RAPD-based B. licheniformis groups. The results of this study will broaden the knowledge about the spoilage potential and thermal resistance of many strains of dairy origin.
研究领域:
食品科学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Zhejiang University-SCIENCE B
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2018
·
IF 1.815
Lei Yuan,Faizan A. Sadiq,Tong-jie Liu,Yang Li,Jing-si Gu,Huan-yi Yang,Guo-qing He
The storage and transportation of raw milk at low temperatures promote the growth of psychrotrophic bacteria and the production of thermo-stable enzymes, which pose great threats to the quality and shelf-life of dairy products. Though many studies have been carried out on the spoilage potential of psychrotrophic bacteria and the thermo-stabilities of the enzymes they produce, further detailed studies are needed to devise an effective strategy to avoid dairy spoilage. The purpose of this study was to explore the spoilage potential of psychrotrophic bacteria from Chinese raw milk samples at both room temperature (28 °C) and refrigerated temperature (7 °C). Species of Yersinia , Pseudomonas , Serratia , and Chryseobacterium showed high proteolytic activity. The highest proteolytic activity was shown by Yersinia intermedia followed by Pseudomonas fluorescens (d). Lipolytic activity was high in isolates of Acinetobacter , and the highest in Acinetobacter guillouiae . Certain isolates showed positive β-galactosidase and phospholipase activity. Strains belonging to the same species sometimes showed markedly different phenotypic characteristics. Proteases and lipases produced by psychrotrophic bacteria retained activity after heat treatment at 70, 80, or 90 °C, and proteases appeared to be more heat-stable than lipases. For these reasons, thermo-stable spoilage enzymes produced by a high number of psychrotrophic bacterial isolates from raw milk are of major concern to the dairy industry. The results of this study provide valuable data about the spoilage potential of bacterial strains in raw milk and the thermal resistance of the enzymes they produce.
研究领域:
食品科学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2014
·
IF 3.259
Peijin Wang,Yu Xiao,Wenjun Liu,Juan Wang,Yaling Yang
A rapid, simple, sensitive and environmentally friendly method has been developed for the determination of three oestrogens (17β-estradiol (17β-E2), estrone (E1), and diethylstilbestrol (DES)) in milk samples by using vortex-assisted hollow fibre liquid-phase microextraction (VA-HF-LPME) and high performance liquid chromatography. Method is based on the microextraction of oestrogens from sample solution into 15 μL of nonanoic acid as extracting agent, which is placed inside the hollow fibre followed by vortex-mixing. Vortex provided effective and mild mixing of sample solution and increased the contact between analytes and boundary layers of the hollow fibre, thereby enhancing mass transfer rate and leading to high recovery of target analytes. The extraction equilibrium is achieved within 2 min. Parameters influencing the recovery were investigated and optimized. The proposed technique provided good linearity (>0.9984), repeatability (RSD = 2.56–4.38), low limits of detection (0.06–0.17 ng mL −1 ), and high enrichment factor (330).
研究领域:
分析化学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
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2017
·Vol. 7
·期 21
·12827-12834
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IF 3.108
Jinping Song,Jing Li,Ziying Guo,Wen Liu,Qi Ma,Feng Feng,Chuan Dong
Casein was employed for the first time as a green carbon source to prepare water-soluble sulfur and nitrogen co-doped carbon dots (S,N-CDs). The as-synthesized S,N-CDs were characterized by transmission electron microscopy, X-ray photoelectron spectroscopy, UV-Vis absorption spectroscopy, and fluorescence spectroscopy. Results showed that the as-prepared S,N-CDs had excellent stabilities under various external conditions, such as high ionic strength, different pH levels, UV light irradiation, and long storage duration. More importantly, we found that doxycycline (Dox) could result in an effective fluorescence quenching of S,N-CDs via a static quenching process. Thus, a sensitive and selective sensing system for Dox detection was developed. The linear range was 1.0 × 10−7 M to 1.0 × 10−4 M, and the detection limit was 25 nm. Furthermore, the proposed sensing system successfully detected Dox in raw milk samples. This research will demonstrate the feasibility and potential applications of carbon nanomaterials in food and medical monitoring fields.
研究领域:
食品科学
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分析化学
·
纳米材料科学
✓ 已验证
产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2021
·
IF 4.527
Mengdi Yuan,Jia An,Guoxiu Zhang,Yongqin Hu,Mengfei Luo,Yuxia Shi,Yufei Liu
Tetracycline (TET) is one of the common antibiotics used in animal husbandry. However, the long-term consumption of TET may increase the risks for human health, especial in dairy products. Moreover, after entering the human body, TET is difficult to be absorbed by the intestines and stomach, and will be discharged into the sewage in the form of the parent compound, which will cause irreversible damage to the natural environment. Novel nitrogen-doped carbon dots (N-CDs) nanoparticles were successfully fabricated using in situ hydrothermal reaction on the inner surface of the aminated central radial silica spheres (CRSSs-NH 2 ), called CRSSs-NH 2 @N-CDs. The aminated central radial carrier played a role in protecting and stabilizing N-CDs, thus enhancing it fluorescence and realizing solid-state light emission. The results shown that the optimal excitation wavelength of CRSSs-NH 2 @N-CDs was about 365 nm, and the emission wavelength was about 446 nm. The CRSSs-NH 2 @N-CDs was then used as a fluorescent probe for rapidly detecting TET. With optimized experimental conditions, the fluorescence quenching intensity of CRSSs-NH 2 @N-CDs had a good linear relationship with the concentration of TET. The linear detection range was 0.5–60 μM, and the limit of detection is 0.39 μM. Furthermore, the TET-sensing application in milk has been carried out, and the average recovery range is 91.90%–104.70%.
研究领域:
分析化学
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环境科学
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食品安全
✓ 已验证
产品关联
DOI
高影响因子
FOOD CONTROL
·
2016
·
IF 3.388
Jing Qin,Xiping Cui,Panpan Wu,Zhengyun Jiang,Yingshan Chen,Ruili Yang,Qingqing Hu,Yuanming Sun,Suqing Zhao
Fluorescent sensor assay (FSA) was developed by using a fluorescein-labeled aptamer assembled onto Graphene oxide (GO) in order to determine β-lactamase in milk. Under optimal conditions, FSA indicated a detection range from 1 to 46 U/mL with a limit of detection (LOD) of 0.5 U/mL ( R 2 = 0.999, n = 3). In addition, commercial milk samples tainted with β-lactamase were detected by the established FSA with a recovery rate between 96.04 and 119.67%. Additionally, the reliability and sensitivity of FSA was validated by enzyme-linked immunoassay (ELISA) with a high correlation of 0.993. Thus, these data, combined with the ease and speed of the assay, suggest that the developed FSA may represent a promising method for monitoring β-lactamase contamination in milk.
研究领域:
生物化学
·
食品科学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Ruifang Zhu,Li Wang,Ailun Chao,Fangyu Fan,Mingfu Wang,Yueliang Zhao
Rose polyphenols, key functional components in roses, require adequate bioaccessibility for their health benefits, subject to influence by food components and processing. Investigating the impact of various thermal sterilization methods on the bioaccessibility of rose polyphenols in rose tea with milk and the underlying mechanisms, our findings indicated a significant increase in bioaccessibility following treatment at 85 °C/30 min. Conversely, 121 °C/15 min treatment decreased bioaccessibility. Examining the interaction between β-casein in milk and rose polyphenols under different sterilization conditions, SEM and particle size analysis revealed binding, with fluorescence spectroscopy indicating non-covalent bonds. Binding forces followed the order 121 °C > 85 °C > 25 °C. Notably, at 85 °C, non-covalent binding improved polyphenol bioaccessibility, while the intensified binding at 121 °C decreased it. SDS-PAGE and amino acid analysis confirmed no covalent bond. This study establishes a theoretical basis for selecting thermal sterilization temperatures for milk-flower combinations, considering polyphenol bioaccessibility.
研究领域:
食品科学
·
植物化学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Aying Wen,Kexin Chi,Changjian Li,Shaofeng Yuan,Hang Yu,Yahui Guo,Weirong Yao
Oxytetracycline (OTC) residues in milk spontaneously bind to casein, forming casein-OTC complexes that complicate their removal. This study assessed the degradation effect of pulsed electric field (PEF) treatment on OTC, with a particular focus on its protein-bound form. Casein-bound OTC was successfully prepared, and the effectiveness of PEF in removing casein-bound antibiotics was confirmed using DESI-MSI technology. Under optimal treatment conditions, the degradation rates of OTC in casein-free whey (86.30 % – 93.43 %) and milk (82.47 % – 90.31 %) showed no significant difference, further confirming that the removal efficacy of PEF on OTC was unaffected by casein binding. The degradation of OTC in both matrices followed the Hülsheger model. Four major degradation products were identified, involving three distinct pathways: epimerization, demethylation, and deamidation. Furthermore, cytotoxicity assays demonstrated that PEF treatment significantly reduced the overall toxicity of OTC in milk. This study provides an effective and safe strategy for removing OTC residues from milk.
研究领域:
食品安全
·
生物化学
·
药物分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Suozhu Wu,Yiqin Zhang,Xuedong Jia,Jie Mao,Hao Wu,Shurong Wang,Hongyuan Guo
Chloramphenicol (CAP) is an antibiotic that could cause serious toxic effects on the human body. Therefore, it is crucial to measure CAP levels in food products. Here we developed an electrochemical sensor based on the molecularly imprinted polymer/polyaniline/gold nanoparticles-reduced graphene oxide-chitosan (MIP/PAN/AuNPs-RGO-CS) modified electrode to determine CAP. The PAN/AuNPs-RGO-CS and MIP film provided the function of signal amplification element and specific recognition unit, respectively. CAP was detected by square-wave voltammetry using potassium ferricyanide (K 3 Fe(CN) 6 ) as a redox signaling probe. The reduction peak current of K 3 Fe(CN) 6 linearly decreased with the logarithmic CAP concentration in two distinct ranges of 1.0–8.0 nM and 10–200 nM with a low limit of detection of 63 pM. This sensor also exhibited short removal and adsorption time, good repeatability, high selectivity, excellent stability, and acceptable recoveries for milk and egg, which offered a promising avenue for ultrasensitively measuring trace antibiotics in actual samples.
研究领域:
分析化学
·
食品安全检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）