JOURNAL OF POLYMER SCIENCE
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2023
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IF 3.4
Difei Zhang,Ziyan Wang,Jieyu Gu,Yan Zhang,Jinghua Chen
Biocatalysis is playing an increasingly important role for chemical, pharmaceutical, and food industries, owing to its high efficiency, selectivity and environment-friendliness. However, it remains challenging to improve the catalytic activity of enzymes since the most frequently used strategies are costly and time-consuming. In the current study, a new type of glycogen-based dynamic nano-frameworks is prepared as additives for the direct activation of carbonic anhydrase ( bovine erythrocytes [bCA]). The boronic acid functionalization of the branched glycogen structures can provide N B coordination with bCA, leading to the conjugation and stabilization of the target enzyme. As a result, the glycogen with a 10% substitution degree ( Gly-PBA 10 ) showed a higher activation effect for bCA than that of glycogen with a 20% substitution degree ( Gly-PBA 20 ). Furthermore, the mechanism for interactions between the dynamic frameworks and bCA has been studied through combined techniques of dynamic light scattering, circular dichroism spectroscopy, and isothermal titration calorimetry. The results suggested different protein binding modes and stabilization abilities for the protein secondary structures from these two types of functionalized dynamic frameworks. This study provided new insights into the protein-polymer interactions that can be beneficial for various biochemical applications. Graphical
研究领域:
生物技术
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生物化学
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酶学
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纳米技术
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DOI
高影响因子
Journal of Environmental Chemical Engineering
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2025
·Vol. 14
·期 1
·120911
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IF 7.2
Yong Xiang,Daoyong Yu,Yi Zhao,Rujing Guo,Jialin Chen,Baosheng Ge,Zijun Xiao
To address the limitations of synthetic zinc porphyrins (ZnP)—including high hydrophobicity, synthesis cost, and poor reusability—as carbonic anhydrase (CA) mimics, this study utilized chlorophyll a -rich Arthrospira platensis algal powder to synthesize hydrophilic zinc and copper chlorophyllin trisodium salts (Zn/Cu-E6–3Na). These derivatives preserve the metal-porphyrin catalytic center while incorporating three carboxylic groups, significantly improving water solubility. To enhance stability and recyclability, ZIF-8 was employed as a carrier to immobilize the Zn/Cu-E6–3Na via coordination, forming Zn/Cu-E6–3Na@ZIF-8 composites. Characterization confirmed anchoring through dual interactions involving both the central metal ion and carboxyl groups, which also improved the hydrophilicity of ZIF-8. The composites considerably enhanced CO 2 absorption within a potassium carbonate system. Esterase activity assays demonstrated a synergistic effect, with Zn- and Cu-composites reaching 0.14 U and 0.13 U, respectively, outperforming individual components. Furthermore, the composites retained over 62 % of their initial activity after multiple cycles without significant metal leaching, indicating excellent operational stability. This work presents a new approach for CO 2 capture using functional chlorophyll derivatives and proposes an innovative strategy for high-value biomass utilization.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
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2023
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IF 15.1
Guanhua Liu,Lirong Wang,Lihang Yan,Hao Zhao,Yaoxuan Li,Liya Zhou,Ying He,Li Ma,Yunting Liu,Jing Gao,Yanjun Jiang
Enzymatic reduction of CO 2 into high-value chemicals has become an emerging technology attributed to its high selectivity and catalytic efficiency under mild conditions. While, the low solubility of CO 2 at room temperature, the expensive cofactors and the poor stability of free enzyme limit the application potential. To solve these problems, we used organic microcapsules together with MOF to protect formate dehydrogenase (FDH) by encapsulation and carbonic anhydrase (CA) by covalent bond with high CO 2 capture ability to construct a dual-enzyme microreactor. Besides, the additional mesopores of the microreactor can ensure the efficient internal diffusion of the substrate and product. Electron mediator was used to enhance electrocatalytic NADH regeneration to efficiently reduce CO 2 to formate in the enzyme electrocatalysis system. After optimization, the highest yield of formate in the electrocatalytic CO 2 reduction system by the dual enzyme microreactor was 2.917 mM with a generation rate of 1.296 × 10 4 μmol g cat -1 h −1 , which was 8.9 times higher than free enzyme.
研究领域:
化学工程
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生物催化
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电化学
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环境科学
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DOI
高影响因子
Molecular Catalysis
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2018
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IF 0
Yueying Han,Sihan Yu,Lichao Liu,Shijia Zhao,Tianxiao Yang,Yajing Yang,Yunming Fang,Shanshan Lv
A significant challenge remains to protect proteins (including enzymes) from inactivation against pH. This study investigated stabilization against pH denaturation of carbonic anhydrase (CA) in silk fibroin (SF)-based hydrogels. CA was immobilized into SF-based hydrogels by a Ru(II) mediated photochemical method, giving rise to ∼100% immobilization efficiency and >60% activity of the free enzyme at the optimum pH value of 8. At an unfavorable acidic pH value of 3, the immobilized CA achieved >20% of the initial activity and ∼8% in the second cycle even after 12 h storage, while the free enzyme lost its activity completely. Fourier transform infrared spectroscopy (FTIR) results suggested that the protective effect of the SF-based hydrogels on the pH stability of CA could be attributed to unique structural feature of the SF molecules, enabling strong intermolecular interactions between SF and CA, and thus increasing protein rigidity against pH denaturation. The same approach was applied to lysozyme and xylanase, and achieved protein stabilization of both enzymes at an unfavorable basic pH value of 9. The SF-based hydrogels demonstrated great potential as a promising matrix for protein immobilization against pH denaturation.
研究领域:
材料科学
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生物化学
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生物技术
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DOI
ANALYTICAL CHEMISTRY
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2024
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IF 6.7
Yinlong Zhu,Zheyi Liu,Jialiang Liu,Heng Zhao,Rui Feng,Kunxian Shu,Fangjun Wang,Cheng Chang
Ultraviolet photodissociation (UVPD) mass spectrometry unlocks insights into the protein structure and sequence through fragmentation patterns. While N- and C-terminal fragments are traditionally relied upon, this work highlights the critical role of internal fragments in achieving near-complete sequencing of protein. Previous limitations of internal fragment utilization, owing to their abundance and potential for random matching, are addressed here with the development of Panda-UV, a novel software tool combining spectral calibration, and Pearson correlation coefficient scoring for confident fragment assignment. Panda-UV showcases its power through comprehensive benchmarks on three model proteins. The inclusion of internal fragments boosts identified fragment numbers by 26% and enhances average protein sequence coverage to a remarkable 93% for intact proteins, unlocking the hidden region of the largest protein carbonic anhydrase II in model proteins. Notably, an average of 65% of internal fragments can be identified in multiple replicates, demonstrating the high confidence of the fragments Panda-UV provided. Finally, the sequence coverages of mAb subunits can be increased up to 86% and the complementary determining regions (CDRs) are nearly completely sequenced in a single experiment. The source codes of Panda-UV are available at https://github.com/PHOENIXcenter/Panda-UV.
研究领域:
分析化学
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蛋白质组学
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质谱学
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生物信息学
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DOI
PubMed
高影响因子
ACS Nano
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2019
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IF 13.903
Tengchuang Ma,Yunduo Liu,Qiong Wu,Lifang Luo,Yali Cui,Xinghua Wang,Xiuwei Chen,Longfei Tan,Xianwei Meng
The development of multifunctional nanoscale radiosensitizers has attracted a tremendous amount of attention, which can enhance the radiosensitization of tumor tissues and reduce unnecessary damage to the surrounding organs. However, the persistent hypoxia environment within the tumor limits their applications in radiotherapy. In this paper, a stable nanocomposite was engineered to overcome the hypoxia properties by using 1,4-benzenedicarboxylic acid produced from a Zr-MOF as a carbonic anhydrase IX (CA IX) inhibitor and quercetin (QU) as a radiosensitizer. QU was encapsulated into the Zr-MOF structure to achieve a synergetic dual sensitization therapy. Zr-MOF-QU exhibits an excellent potential of radiotherapy sensitization characteristics in vitro and in vivo from the γ-H2AX immunofluorescence staining and colony assays. The mechanisms of alleviating hypoxia-induced resistance and sensitizing tumor tissues to improve cell apoptosis from radiation were found to suppress CA IX expressions by the decomposition product from Zr-MOF and boost the sensitivity by QU in radiation therapy. Moreover, there was no significant systemic toxicity during the treatment, and the therapeutic outcome was assessed in animal models. Therefore, our results demonstrate a promising cancer treatment approach in the radiation field.
研究领域:
癌症治疗
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纳米技术
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放射生物学
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药学
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DOI
PubMed
高影响因子
Nanoscale
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2019
·Vol. 11
·期 13
·5960-5966
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IF 6.97
Jinxing Chen,Liang Huang,Qingqing Wang,Weiwei Wu,He Zhang,Youxing Fang,Shaojun Dong
Nanozymes provide comparative advantages over natural enzymes and conventional artificial enzymes for catalytic reactions. However, nanozymes are only suitable for limited types of reactions, whose catalytic principles are not yet fully revealed. Herein, a new nanozyme based on a bionic zeolitic imidazolate framework is proposed. Zeolitic imidazolate framework-8 (ZIF-8) possesses a similar geometric structure to that of the active center of human carbonic anhydrase II (hCAII) and exhibits catalytic performance analogous to that of the hCAII. The less imidazolate coordinated zinc cations on the external surface of ZIF-8 can act as Lewis acid sites, lowering the pKa of Zn-bound H2O molecules from 14 to 8.4, which facilitates the deprotonation of H2O molecules and generation of zinc-bound hydroxide nucleophiles. The esterase-like ZIF-8 nanozyme shows a similar affinity to p-nitrophenyl acetate compared with hCAII. The ZIF-8 nanozyme also promotes CO2 hydration and acetylthiocholine hydrolysis reaction, and a series of ZIFs are also found with intrinsic enzyme-like activities due to similar compositions and spatial structures. These results imply that the bionic nanoparticles can be developed to fabricate a new generation of nanozymes by mimicking the active sites of natural enzymes.
研究领域:
催化
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材料科学
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生物化学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
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2023
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IF 8.4
Fuying Zhu,Haochen Qiu,Fei Wang,Xing Zhang,Guo-Ping Lu,Yamei Lin,He Huang
CO2 conversion by stable, cost-effective carbonic anhydrase (CA)-like nanozymes emerges as an efficient and sustainable approach for CO2 fixation. In this work, a novel iron-based nanomaterial (Fe10@CN-Mg) was first reported to be a CA mimic, in which FeNx sites and Mg(OH)2 play a synergistically catalytic effect for CO2 conversion. Although this material has much lower metal content and cost, it has comparable kinetic constants (Km 6.37 mM and Vmax 30.74 mM/min) and a significantly higher CaCO3 formation rate (20.60 g·g–1·h–1, the quality of CaCO3 produced per hour per gram of the catalyst) than that of reported CA mimics. Fe10@CN-Mg is stable when processed at extreme pH, high temperature, organic solvents, and high ionic strength and also retains high activity after long storage times (two months) and seven cycles. In addition, Fe10@CN-Mg was successfully applied to convert CO2 into cyclic carbonates with high economic efficiency.
研究领域:
材料科学
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环境化学
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催化
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可持续能源
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2022
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IF 16.744
Menglei Mao,Tingting Zhai,Lingding Meng,Zihui Meng,Wenfang Liu
Enzymatic conversion of CO 2 to high-value chemicals is a significant route for the utilization of greenhouse gases in mild, high-selectivity and environment-friendly way, however, conversion efficiency is not yet satisfying. Here, mesoporous silica (mSiO 2 ) nanoparticles with controllable structure were prepared and modified by polydopamine (PDA) and polyethyleneimine (PEI), which were then used in an integrated process for CO 2 capture and conversion to formate for the first time. The effects of structure parameters of mSiO 2 and modification conditions on its application properties were investigated. The results show that in a range of 230 ∼ 500 nm, SiO 2 particles with smaller size exhibited better intensifying effect, while the optimal size was 410 nm for mSiO 2 with the same etching extent, attributed to a higher specific surface area. mSiO 2 itself was a robust CO 2 adsorbent and the addition of 0.01 g mSiO 2 (410) enabled CO 2 conversion to be accelerated 11.94 times compared to free enzyme. After modification, with 0.05 g PDA/PEI-mSiO 2(340) and PDA/PEI-mSiO 2(410) , the enzyme reaction was expedited up to 24 and 30.8 times of the system without particles due to an enhanced CO 2 uptake, as a collaborative result of mesoporous structure and amino-functionalization. Following that, PDA/PEI-mSiO 2(410) was used as the carrier for the co-immobilization of formate dehydrogenase and carbonic anhydrase, which could retain 86.7% activity after use for 10 times and 55.2% activity after 21 days at 4 °C, while 29.6% for free enzymes.
研究领域:
材料科学
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环境科学
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生物催化
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化学工程
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DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2022
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IF 3.191
Guo-Ying Chen,Xi Zhou,Tao Tian,Chun-Yan Zhang,Shi-Jun Yin,Hua Chen,Yi Xu,Feng-Qing Yang
A simple and time-saving colorimetric method was developed to quantify sulfonamides (SAAs) in milk via inhibition of the human carbonic anhydrase II (hCAII)-like activity of ZIF-8 that can hydrolyze p -nitrophenyl acetate ( p NPA) to p -nitrophenol ( p NP), following the color change from yellow to colorless. Effects of different reaction conditions, including pH, temperature, amount of ZIF-8, and incubation time , were investigated. The value of Michaelis-Menten constant ( K m ) is measured to be 0.15 mM, which exhibits high affinity to p NPA. The IC 50 (0.17, 0.24, and 0.60 mM) and inhibition constant ( K i ) (0.09, 0.13, and 0.33 mM) of sulfamethazine (SD), sulfadimethoxine (SDM), and sulfathiazole (ST) on ZIF-8 were measured, respectively. Moreover, the activity of ZIF-8 remains more than 90.0% of its initial activity after 30 days’ storage. The colorimetric method for SD, SDM, and ST determination was established at the linear ranges of 6.3–750.0 μM (1.75–208.75 mg/kg), 6.3–750.0 μM (1.96–232.75 mg/kg), and 5.0–1250.0 μM (1.28–319.15 mg/kg) with limit of detection of 4.3, 3.2, and 3.9 μΜ (1.2, 0.99, and 0.96 mg/kg), respectively. In addition, the spiked recoveries of SAAs in milk sample are in the range of 81.6%–106.7% with RSD less than 6.5%. In short, the developed colorimetric method can achieve rapid analysis of SAAs in milk with simple operations.
研究领域:
分析化学
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食品科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
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2025
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IF 7.3
Zhao Cui,Liang Lu,Jing Sun,Qian Li,Ting Li,Guangyue Li
CO2 + O2 neutral leaching represents a promising sustainable method for uranium extraction; however, its practical effectiveness is frequently limited by the slow kinetics of CO2 hydration, which hinders the formation of bicarbonate ions essential for the complexation and dissolution of uranium. To overcome this kinetic limitation, a novel magnetic zinc-coordinated carbonic anhydrase-mimicking nanozyme (Fe3O4@ZnAC) was successfully developed via a facile one-pot sol–gel coating strategy. Comprehensive characterization confirmed that the nanozyme exhibits a distinctive core–shell heterostructure, consisting of a superparamagnetic Fe3O4 core enabling facile magnetic recovery and a zinc-rich organosilicate shell providing abundant, accessible Zn–N/O catalytic sites analogous to natural carbonic anhydrase. Enzymatic studies revealed that Fe3O4@ZnAC possesses outstanding carbonic anhydrase-mimicking catalytic performance, robust operational stability, and excellent recyclability, highlighting its potential for sustainable industrial applications. When integrated into a dual-reactor uranium leaching system, Fe3O4@ZnAC substantially increased bicarbonate ion production by approximately 47% compared to the uncatalyzed scenario. This catalytic improvement resulted in a uranium extraction efficiency of 45.79%, significantly higher than the 36.36% observed in control experiments. This study provides valuable insights into the rational design and practical application of biomimetic nanozymes, proposing an effective strategy for integrating efficient resource recovery with sustainability and global carbon neutrality goals.
研究领域:
纳米技术
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酶模拟物
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环境科学
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无机化学
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材料科学
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可持续化学
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生物无机化学
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工业催化
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产品关联
DOI
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
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2020
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IF 2.627
Jing Wang,Yongqin Lv
Ultrathin polydopamine microcapsules with hierarchical structure and porosity were prepared for the immobilization of multienzymes using metal-organic framework (MOF) as the template. The multienzyme/MOF composite was first prepared using a “one-pot” co-precipitation approach via the coordination and self-assembly of zinc ions and 2-methylimidazole in the presence of enzymes. The obtained nanoparticles were then coated with polydopamine thin layer through the self-polymerization of dopamine under alkaline condition. The polydopamine microcapsules with an ultrathin shell thickness of ~ 48 nm were finally generated by removing the MOF template at acidic condition. Three enzymes were encapsulated in PDA microcapsules including carbonic anhydrase (CA), formate dehydrogenase (FateDH), and glutamate dehydrogenase (GDH). FateDH that catalyzed the main reaction of CO 2 reduction to formic acid retained 94.7% activity of equivalent free FateDH. Compared with free multienzymes, the immobilized ones embedded in PDA microcapsules exhibited 4.5-times higher of formate production and high catalytic efficiency with a co-factor-based formate yield of 342%.
研究领域:
化学工程
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材料科学
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生物化学
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环境科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）