Analytical Methods
·
2013
·Vol. 5
·期 18
·4716-4722
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IF 1.855
Bo Yang,Wenhui Wu
We have designed and synthesized a spirobifluorene–tetrrhodamine fluorescent probe 2 based on the through-bond energy transfer (TBET) system for the “off–on” detection of Hg2+. As compared with fluorescence resonance energy transfer (FRET) strategy, the TBET system did not need spectral overlap between the donor emission and the acceptor absorption, therefore, the choice of dye pairs for the probe design was wide. The Stokes shift was larger and the energy transfer efficiency was higher than that of the FRET system. The probe 2 showed exclusive response toward Hg2+ by the fluorescence enhancement and the color changed from colorless to pink. Other metal ions showed no significant variation to Hg2+. The energy-transfer efficiency was calculated to be 99.6%, and the detection limit was determined to be 3 ppb. Thus, probe 2 could be used as a fluorescent and colorimetric chemosensor for the detection of Hg2+.
研究领域:
化学
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分析化学
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环境科学
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DOI
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 22
·9073-9077
·
IF 1.938
Yu Zhang,Liqian Wu,Yurong Tang,Yingying Su,Yi Lv
A new fluorescent probe for detecting lead ions (Pb2+) is reported in this work. Positively charged UCPs capped with ethylene imine polymer (PEI) and negatively charged AuNPs capped with 11-mercaptoundecanoic acid (MUA) were used as fluorescent donors and acceptors, respectively. The fluorescence quenching process was initiated by the electrostatic interaction between PEI–UCPs and MUA–AuNPs. Upon the addition of Pb2+, functionalized AuNPs were aggregated by an ion-templated chelation process, causing the departure of AuNPs from UCPs. As a result, the fluorescence of the donor was restored and the recovery was found to be proportional within the range of 0.5–10 μM to the concentration of Pb2+. The detection limit was found to be 20 nM. This method was easy to operate with satisfactory selectivity and remarkable sensitivity which would be meaningful in Pb2+-related detection.
研究领域:
分析化学
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环境科学
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DOI
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 31
·17254-17262
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IF 2.936
Quan Xu,Wei Cai,Miaoran Zhang,Rigu Su,Yingchun Ye,Yeqing Li,Lipeng Zhang,Yongjian Guo,Zhiqiang Yu,Siyu Li,Xun Lin,Yusheng Chen,Yan Luo,Jason Street,Meng Xu
Heteroatom-doped carbon dots (CDs) with excellent optical characteristics and negligible toxicity have emerged in many applications including bioimaging, biosensing, photocatalysis, and photothermal therapy. The metal-doping of CDs using various heteroatoms results in an enhancement of the photophysics but also imparts them with multifunctionality. However, unlike nonmetal doping, typical metal doping results in low fluorescence quantum yields (QYs), and an unclear photoluminescence mechanism. In this contribution, we detail results concerning zinc doped CDs (Zn-CDs) with QYs of up to 35%. The zinc ion charges serve as a surface passivating agent and prevent the aggregation of graphene π–π stacking, leading to an increase in the QY of the Zn-CDs. Structural and chemical investigations using spectroscopic and first principle simulations further revealed the effects of zinc doping on the CDs. The robust Zn-CDs were used for the ultra-trace detection of Hg2+ with a detection limit of 0.1 μM, and a quench mechanism was proposed. The unique optical properties of the Zn-CDs have promise for use in applications such as in vivo sensing and future phototherapy applications.
研究领域:
材料科学
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光学
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生物医学工程
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DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 115
·95223-95229
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IF 3.84
Xiaoqing Niu,Guosheng Liu,Luyao Li,Zheng Fu,Hang Xu,Fengling Cui
Fluorescent carbon-based nanomaterials have attracted tremendous concern owing to their unique properties of chemical stability, excellent biocompatibility, tunable excitation and emission spectra, low toxicity and photostability. Herein, a green, simple and low-cost approach was present to obtain nitrogen-doped carbon dots (N-doped C-dots) with the quantum yield of 37.5% using vegetables as the sole carbon source through facile one-pot hydrothermal treatment without additional solvents. The as-prepared N-doped C-dots are fully characterized by high-resolution transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, UV-vis absorption, and fluorescence spectroscopy. The synthesized N-doped C-dots displayed excellent water solubility and stability in a wide range of pH and ionic strength. As the emission of N-doped C-dots is efficiently quenched by Cu2+, the C-dots can serve as a suitable sensing platform for label-free sensitive and selective detection of Cu(II) ions with a detection limit of 9.98 nM. The cell viability results of HeLa cells proved the low toxicity of C-dots. Further, the imaging of Escherichia coli (E. coli) cells and HeLa cells demonstrated the biolabeling potential in vivo of the synthesized C-dots with low toxicity and good biocompatibility.
研究领域:
材料科学
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分析化学
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生物医学工程
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产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2020
·Vol. 412
·期 12
·2893-2901
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IF 3.637
Chen Jingqi,Liu Xiaowei,Hou Xialing,Chen Yingying,Xing Feifei,Feng Lingyan
A label-free fluorescent nanoprobe for iodide ion (I − ) detection was developed based on the direct fluorescence quenching of spermine-functionalized carbon dots (SC-dots), whether in complex biological fluids or living cells. The positively charged SC-dots were fabricated via one-step microwave synthesis and exhibited excellent optical properties. Due to the strong quenching ability of I − , SC-dots were utilized for I − detection with high sensitivity and excellent selectivity, which offered a relatively low detection limit of 0.18 μM. This strategy was also successfully applied for I − detections in human serum and HeLa cells. The detection process is facile, highly sensitive and selective, providing a new insight into the potential applications of SC-dots for anion nanoprobe designs in clinical diagnosis and other biologically related areas.
Graphical abstract
研究领域:
分析化学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2019
·
IF 7.015
Yuan Wang,Zhuang Liu,Feng Luo,Han-Yu Peng,Shu-Gui Zhang,Rui Xie,Xiao-Jie Ju,Wei Wang,Yousef Faraj,Liang-Yin Chu
A novel smart membrane with ion-recognizable nanogels as gates on interconnected pores is developed for simple and rapid detection of trace lead(II) ions in water. The membrane is prepared via one-step vapor-induced phase separation (VIPS) method, and featured with interconnected porous structure and Pb 2+ -responsive poly( N -isopropylacrylamide- co -benzo-18-crown-6-acrylamide) (PNB) nanogels on polyethersulfone (PES) membrane pore surfaces as gates. Afforded by such a unique architecture, both high flux and significant responsive characteristics are achieved. With the proposed strategy, the nanogels are fixed onto the membrane pore surfaces during the formation of interconnected porous structure; thus, the immobilization of nanogels onto the membrane pore surfaces does not result in the decrease of pore sizes. The water fluxes of fabricated membranes could be even higher than 6000 kg m −2 h −1 driven by the static pressure of 20 cm water column under gravity at 34 °C, because of the three-dimensionally interconnected porous structures. Due to high sensitivity and selectivity of PNB nanogels on the membrane pore surfaces in response to Pb 2+ ions, the fabricated membranes can efficiently detect Pb 2+ ions with a limit of detection as low as 10 −9 mol/L. With the proposed novel membrane, rapid detection of trace lead(II) ions in water can be easily achieved by simply monitoring the trans-membrane flux change in a gravity-driven membrane module device, which is highly potential and promising in real-time and easy-to-popularize monitoring of drinking water safety and may expand the scope of applications of facile detection techniques.
研究领域:
材料科学
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环境科学
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分析化学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
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2022
·
IF 5.304
Jiao Yang Lu,Zhen Qi Bu,Wei Tao Huang
Peptides are a class of powerful molecules and building blocks similar to DNA. However, peptides with composition diversity and molecular recognition ability are rarely used for multipurpose integrated applications (especially in sensing, information processing, communication, and security). Herein, peptide-based sensing of Pb 2+ , logic computing, information encoding, and security applications were comprehensively demonstrated. Our fluorescently labeled Pb 2+ -binding peptide exhibited different responses to various metal ions but was specific to Pb 2+ , which were attributed to the fluorescence static quenching and the transformation of the peptide conformation induced by Pb 2+ . This peptide probe was successfully used to quantitatively detect Pb 2+ (detection limit 3.38 nM) in actual water samples and to perform logic calculations. In addition, due to natural concealment of molecules, encoding, encryption, and hiding of 2 kinds of dimensional information (such as text and images), were displayed by using peptide sequences and its selectivity. This information encoding method based on molecules and their subsidiary properties shows great flexibility and scalability and provides a new option for information representation and protection. Inspired by this idea, more interesting and creative molecular sensing and information systems will be developed to promote connectivity, programmability, and intelligence of the molecular world.
研究领域:
生物化学
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分子生物学
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信息科学
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DOI
高影响因子
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
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2022
·Vol. 43
·期 12
·1373-1382
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IF 1.241
Wen-Kai Zhu,Wen-Rong Cai,Zhen-Zhi Yin,Ming-Jie Cheng,Yong Kong
A novel Zn-coordination covalent porphyrin assembly (TCPP-BZA-Zn) is designed. The assembly structure is synthesized through the amidation reaction between the porphyrin terminal carboxyl group and the amino group of benzylamine (BZA), and further assembled through π–π stacking. In particular, the inherently ordered structure of TCPP-BZA-Zn with Zn as the catalytic active center endows the porphyrin assembly structure with several obvious advantages, such as high ion transport properties and high electrocatalytic performance. In the presence of hydrogen peroxide as a co-reaction reagent, TCPP-BZA-Zn/GCE showed excellent ECL behavior. The amplification phenomenon of ECL was further studied by cyclic voltammetry and the corresponding mechanism was proposed. Based on TCPP-BZA-Zn, an electrochemiluminescence sensor was constructed for copper ion detection. The ECL intensity of the sensor shows a good linear relationship with the concentration of copper ion in the range of 10 nM–1 mM, and the detection limit is 1.3 nM.
研究领域:
材料科学
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分析化学
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DOI
高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 26
·3233-3239
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IF 3.1
Jian Song,Xiaoqin Yang,Sida Xie,Guolei Zhu,Xiaoping Rao,Ping Zhao,Qian Jiang
The specificity detection of Al3+ is important for monitoring life health and environmental pollution. A fluorescence enhancement probe based on caffeic acid HAM was synthesized for detecting Al3+ with high sensitivity and good selectivity. When Al3+ was added in the aqueous solution of HAM, the formation of HAM–Al3+ complexes inhibited the PET process, which led to great enhancement of fluorescence. The addition of other metal ions cannot induce the change of fluorescence intensity. The sensing mechanism was proved by 1H NMR titration, MS, and Job's plot. Moreover, probe HAM exhibited excellent properties, such as high sensitivity (LOD = 0.168 μM), fast response time (30 s), wide pH range (3–11), and good interference ability. Based on the above results, probe HAM was used to explore its bioimaging application in biological samples.
研究领域:
分析化学
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生物医学
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环境科学
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 9
·6824-6830
·
IF 3.201
Guojuan Ren,Yuxi Meng,Qi Zhang,Mingyu Tang,Baoya Zhu,Fang Chai,Chungang Wang,Zhongmin Su
Nitrogen-doped carbon dots (NCDs) were synthesised using a simple and straightforward solvothermal method. The resulting spherical NCDs showed stable fluorescence and a prominent fluorescence quantum yield (67%) with an average size of 3.52 nm. The fluorescence of the NCDs was quenched effectively by mercury ions (Hg2+), exhibiting a detection limit of 0.65 μM. Upon addition of a saturated solution of ethylenediaminetetraacetic acid to a dispersion of the NCDs/Hg2+ complex, the fluorescence of the NCDs underwent significant immediate recovery. Due to their favorable biocompatibility, NCDs were also demonstrated to be efficient for HeLa cell imaging. The detection of Hg2+ by NCDs could be realized in environmental water samples and living cells, with excellent results. Moreover, the as-synthesized fluorescent NCD nanopowders were used for visualizating latent fingerprints (LFPs) with significant third level detail.
研究领域:
生物化学
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环境科学
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材料科学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Ke Jian,Liming Fu,Yujie Zhang,Hongmei Zhang,Xiangjun Guo,Xihui Zhao
Yellow fluorescent carbon dots (Y-CDs) were prepared via microwave method using chitosan and o -phenylenediamine as the main raw materials. The obtained Y-CDs possesses good water solubility, excellent biocompatibility and luminous stability. During the microwave pyrolysis carbonization process, the surface of Y-CDs was modified with the functional groups such as amino and carboxyl, which can bind to Al 3+ by forming complexes, further improving the selectivity and sensitivity of the Al 3+ detection. And the fluorescence of Y-CDs was quenched by Al 3+ by static quenching process. More importantly, Y-CDs as fluorescent sensor was further applied for the determination of Al 3+ in the real water samples with high reliability and accuracy. In addition, Y-CDs present potential application in biological imaging. The cultivated zebrafish embryos with Y-CDs displayed clearly in vivo uptake and metabolic fluorescence images, further confirming its low toxicity and excellent biocompatibility.
研究领域:
材料科学
·
分析化学
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生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Xiang Deng,Huan Yang,An-Jie Gong,Xiao-Mei Huang,Lu Li
The excessive accumulation of heavy metal ions can pose significant hazards to human health and the environment, hence the detection and removal of heavy metal ions from water bodies are essential. Generally, carbon dots (CDs) are used as a good fluorescence sensor to sense heavy metal ions. In this study, using one-step microwave method, CDs were synthesized with the traditional Chinese medicine Radix Vladimiriae as a carbon source for the first time. These CDs display excellent fluorescence properties with an average particle size of approximately 5.9 nm and a fluorescence quantum yield (QY) about 0.15. The fluorescence emission spectra were recorded at different excitation peaks with the highest intensity emission peak observed at 465 nm with an excitation wavelength of 360 nm. Interestingly, fluorescence intensities of CDs greatly decreased in the existence of Fe 3+ . Under optimum conditions, a good linear relationship was observed between ( I 0 - I )/ I and the Fe 3+ concentration in the range of 0.2–200 μM with the correlation coefficient ( R 2 ) of 0.9992 and the limit of detection (LOD) of 62 nM. Finally, after adding Fe 3+ at concentrations of 5 μM, 20 μM, and 50 μM to real water samples, the relative standard deviation and spiked recovery rates were 1.2 %-2.7 % and 99.2 %-101.6 %, respectively. Thus, a novel method for rapid detection of Fe 3+ were successfully discovered.
研究领域:
环境科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
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