MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 5
·1-10
·
IF 6.408
Ding Jinglei,Zhang Wenqi,Xue Fengying,Sun Yu,Yan Quizhu,Chen Yanwei,Shan Guiye
Gold nanoclusters (AuNCs) are widely used in the fluorescence detection of biomolecules in human serum due to their good fluorescence properties, low toxicity, and better biocompatibility. However, the weak fluorescence intensity of AuNCs limits the fluorescence detection of molecules within a wide concentration range. It is reported that coating AuNCs in ZIF-8 with adjustable pore size can effectively improve the fluorescence intensity of AuNCs and broaden the detection range. And the AuNCs wrapped in the gaps of ZIF-8 can prevent the fluorescence quenching caused by the aggregation of AuNCs. However, ZIF-8 has high crystallinity, poor dispersion, and easy deposition, which reduces the fluorescence stability of the detection system and affects the detection. Based on the above research, the highly hydrophilic polymer PEI was modified to the surface of ZIF-8, and a kind of nanocomposite material AuNCs/ChOx@ZIF-8/PEI was obtained by co-encapsulating AuNCs prepared with glutathione as a ligand and cholesterol oxidase (ChOx) into ZIF-8 modified with PEI. The composite material emits strong red light at 650 nm under the excitation of 395-nm light, and the system can sensitively detect cholesterol (Chol) in human serum. Compared with other materials, the PEI-modified composite has better solubility and stability, so the detection effect of Chol is better. Encapsulation of ChOx in the ZIF-8 shell can protect the enzyme and increase the local concentration of ChOx, thereby speeding up the reaction rate. Compared with free AuNCs/ChOx, the quenching rate of AuNCs/ChOx@ZIF-8/PEI system is doubled. Secondly, the addition of Fe 2+ to the detection process results in higher quenching rate and detection sensitivity. The system can detect Chol in the concentration range 0.1–2.4 μM, with a detection limit of 0.073 μM. The determination is a fast and sensitive strategy. In addition, the practicability of this assay in the detection of Chol in human serum has been verified. Due to its selectivity and sensitivity, it has potential application value in clinical diagnosis. Graphical abstract
研究领域:
生物化学
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医学诊断
·
纳米材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Polymer Chemistry
·
2022
·Vol. 14
·期 5
·573-586
·
IF 5.364
Xinjin Yan,Meng Wei,Xu Miao,Xiaoguang Qiao,Xiaomeng Zhang,Peng Fu,Xinchang Pang,Minying Liu,Qingxiang Zhao,Shuang Chen,Zhe Cui
Luminescence of non-conjugated polymers attracts much attention due to its importance in fundamental and applied research. At present, however, the research on nonconventional luminescent polymers (NLPs) in terms of both the structure regulation and emission mechanism seems to be still in its infancy. Herein, we report for the first time that PA1212, a representative engineering plastic with the longest saturated carbon chain among commercial polyamides, is an efficient NLP, which exhibits dual emission of blue fluorescence and persistent room-temperature green phosphorescence as well as high quantum yield (∼13%). Systematic photophysical characterization demonstrates the important role played by hydrogen-bonded amide groups in the emission, and the emission intensity shows a clear linear positive correlation with the amide clusters constructed from ordered hydrogen bonds in the crystalline region. Moreover, PA1212 in the α form with linearly extended hydrogen-bonded clusters exhibits longer emission wavelengths than in the γ form, owing to the narrower band gap between the valence band maximum and the conduction band minimum. Further variable temperature fluorescence studies reconfirm the amide cluster-triggered emission (CTE) mechanism of PA1212, and show the possibility of application of fluorescence spectra in the study of polyamide aggregation states. This work not only deepens the understanding of CTE, but also provides a new strategy to regulate the luminescence of polyamides, and meanwhile, highlights the application prospects of luminescent polyamides.
研究领域:
材料科学
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光物理学
✓ 已验证
产品关联
DOI
高影响因子
Biosensors-Basel
·
2023
·Vol. 13
·期 9
·854
·
IF 5.4
Yang Qing,Haobin Fang,Yuxing Yang,Yazhen Liao,Haiyu Li,Zhencui Wang,Jie Du
At present, a large number of studies have demonstrated that miRNAs can be used as biological indicators for the diagnosis and treatment of diseases such as tumours and cancer, so it is important to develop a new miRNA detection platform. In this work, miRNA-122 is used as the basis for targeting detection agents. We have designed an unlabelled DNA1 that undergoes partial hybridisation and has a 20 T base long strand. The fluorescent signal in this experiment is derived from copper nanoclusters (CuNCs) generated on the circular T-long strand of DNA1. At the same time, DNA1 is able to react with miRNA-122 and achieve hydrolysis of the part bound to miRNA-122 via the action of nucleic acid exonuclease III (Exo III), leaving a part of the DNA, called DNA3, while releasing miRNA-122 to participate in the next reaction, thus achieving circular amplification. DNA3 is able to react with DNA2, which is bound to streptavidin magnetic beads (SIBs) and separated from the reaction solution via the application of a magnetic field. Overall, this is a fluorescence signal reduction experiment, and the strength of the fluorescence signal from the copper nanoclusters can determine whether the target miRNA-122 is present or not. The degree of fluorescence reduction indicates how much DNA1, and thus the amount of target miRNA-122, has been hydrolysed. By evaluating the variations in the fluorescence signal under optimised conditions, we discovered that this method has good sensitivity, with a detection limit as low as 0.46 nM, better than many other previous works on fluorescence signal-based biosensors for miRNA detection. This technique offers high discrimination and selectivity and can serve as a persuasive reference for early diagnosis.Keywords:exonuclease III;miRNA-122;CuNCs;fluorescence reduction
研究领域:
分子生物学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2023
·
IF 6.7
Xingxing Huang,Yingzhang Shi,Cheng Liu,Zhiwen Wang,Jinhong Bi,Jimmy C. Yu,Ling Wu
A series of Cu 2 O clusters/MIL-100(Fe) heterojunctions with different Cu 2 O mass ratios were constructed as the photocatalysts for N 2 fixation. An optimal nitrogen fixation performance of 51.22 μmol·g −1 ·h −1 is achieved when the mass fraction of Cu 2 O is 20 %. Isotope analysis experiments confirmed that the nitrogen source is N 2 . Possible intermediate signals during photocatalytic reactions were detected by in situ DRIFTS. XPS results further confirm the formation of Cu 2 O/MIL-100(Fe) heterojunctions, and that the existence of Cu–O surface interactions is the key to efficient electron transfer. It is revealed that the improvement of the catalytic performance is derived from the formation of heterojunction which regulates the energy band structure as well as promotes the separation of photogenerated electron-hole pairs. This study proposes a possible mechanism of photocatalytic nitrogen fixation and emphasizes the role of surface interaction and electron transfer in type-II heterojunction in improving nitrogen fixation performance.
研究领域:
材料科学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
Magnetochemistry
·
2025
·Vol. 11
·期 9
·70
·
IF 2.5
Ya-Wei Geng,Tong Guo,Xiao-Qin Wang,Tian Han
As an important branch of lanthanide-based complexes, clusters show unique properties in magnetocaloric effect (MCE) and single-molecule magnets (SMMs) using O/N ligands, while research on heavy p-block elements (e.g., S atom) with larger atomic radii and more diffuse p valence orbitals as coordinating atoms remains relatively scarce. Herein, using the sulfur-containing ligand of 2-pyridinethiol 1-oxide (HL), we successfully synthesized a series of hourglass-like Ln9clusters [Ln9(L)17(μ3-OH)9(μ4-OH)]·nH2O (1: Ln = Gd,n= 3;2: Ln = Tb,n= 3;3: Ln = Dy,n= 1). Magnetic data analysis reveals that cluster1shows a significant MCE, with the entropy change (−ΔSm) reaching a maximum of 34.41 J kg−1K−1at 2 K under ΔH= 7 T. Cluster3, meanwhile, exhibits distinct frequency- and temperature-dependent behavior, indicating its SMM characteristics. Interestingly, despite possessing the highest molar mass among reported Gd9clusters with MCE,1exhibits a competitive −ΔSmvalue, highlighting the critical role of sulfur-containing ligand on the structure and even exchange interactions. This work offers new insights into synthesizing high-performance MCE materials and understanding magneto-structural relationships.
研究领域:
无机化学
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分子磁性
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材料科学
·
配位化学
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2019
·Vol. 29
·期 39
·1904144
·
IF 15.621
Zhanwei Zhou,Yadong Liu,Minghua Zhang,Chenzi Li,Ruoxi Yang,Jing Li,Chenggen Qian,Minjie Sun
Protein-based theranostic agents (PBTAs) exhibit superior performance in the diagnosis and therapy of cancers. However, the in vivo applications of PBTA are largely limited by undesired accumulation, penetration, or selectivity. Here, an ATP-supersensitive protein cluster is fabricated for promoting PBTA delivery and enhancing magnetic resonance imaging (MRI)-guided tumor photothermal therapy. Gd 3+ - and CuS-coloaded small bovine serum albumin nanoparticles (GdCuB) are synthesized as the model protein with a size of 9 nm and are encapsulated into charge switchable polycations (DEP) to form DEP/GdCuB nanoclusters of 120 nm. In blood circulation, DEP/GdCuB significantly extends the half-lifetime and thereby enhances the tumor accumulation of GdCuB. When the clusters reach the tumor site, the extracellular adenosine triphosphate (ATP) can effectively trigger the release of GdCuB, resulting in tumoral deep penetration as well as the activation of T 1 -weighted MRI ( r 1 value switched from 2.8 × 10 −3 to 11.8 × 10 −3 m −1 s −1 ). Furthermore, this delivery strategy also improves the tumoral photothermal therapy efficacy with the MRI-guided therapy. The study of ATP-activated nanoclusters develops a novel strategy for tumor deep penetration and on/off imaging of PBTA by size switchable technology, and reveals the potential for MRI-guided therapy of cancers.
研究领域:
纳米技术
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生物医学工程
·
癌症治疗
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磁共振成像
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药物递送系统
✓ 已验证
产品关联
DOI
高影响因子
Aggregate
·
2023
·Vol. 4
·期 5
·e351
·
IF 18.8
Zixiang He,Su Shen,Gong Zhang,Tengfei Miao,Xiaoxiao Cheng,Zhao Wang,Qingping Song,Wei Zhang
The preparation of monodisperse azobenzene (Azo) polymer microspheres by dispersion polymerization was reported. The photo-induced fusion of Azo polymer microspheres was successfully achieved during the process of reversible trans - cis - trans photoisomerization of Azo units, and induced various unique “microsphere molecular” clusters or “microsphere polymers”. Encouraged by this interesting phenomenon, microsphere clusters of different topological structures were stabilized by the in situ acetal cross-linking chemistry. The photo-induced fusion polymerization of monodisperse polymer microspheres provides a new strategy for designing photo-responsive clusters and allows for control over the mechanical properties of microspheres with high spatiotemporal resolution.
研究领域:
材料科学
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聚合物化学
·
光化学
✓ 已验证
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2020
·Vol. 56
·期 7
·4909-4921
·
IF 3.553
Zheng Shanshan,Zhang Qianqian,Yin Danyang,Gu Hongzhi,Zhang Jinli,Li Wei,Fu Yan
Reduced coenzyme II acts as both reducing and capping agents in the growth of AgPd nanoclusters within a suitable range of [K 2 Pd(NO 2 ) 4 ]/[AgNO 3 ]. A remarkable acceleration occurs in the growth kinetics of AgPd nanoclusters with respect to monometallic Pd or Ag. AgPd nanoclusters display a marked electron donation from Ag to Pd, thereby contributing to the enhancement of metallic Pd 0 atoms. The AgPd nanoclusters exhibit better peroxidase mimicking activities relative to their monometallic counterparts. Addition of iodide ions significantly inhibits the peroxidase mimicking activity of Ag 1 Pd 1 nanozyme. Hence, the Ag 1 Pd 1 -catalyzed oxidation of 3,3′,5,5′-tetramethylbenzidine by H 2 O 2 is greatly suppressed. The peroxidase-like activity of Ag 1 Pd 1 nanozyme exhibits a sensitive response to iodide ions in the range of 0.5 ~ 180 nM. AgPd nanozyme possesses a desirable selectivity toward iodide ions with respect to sulfide ions. This work would provide guidance for developing biomimetic nanomaterials in the areas of biosensing, separation, nanotechnology and green synthesis. Graphical abstract
研究领域:
纳米技术
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生物传感
·
催化
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of the American Chemical Society
·
2023
·
IF 15
Qinzhen Li,Yesen Tan,Baoyu Huang,Sha Yang,Jinsong Chai,Xiaoping Wang,Yong Pei,Manzhou Zhu
Unraveling the evolution mechanism of metal nanoclusters is of great importance in understanding the formation and evolution of metallic condensed matters. In this work, the specific evolution process between a pair of gold nanocluster (Au NC) isomers is completely revealed by introducing hydride ligands to simplify the research system. A hydride-containing Au NC, Au22(SR)15H, was synthesized by kinetic control, and the positions of the hydrides were then confirmed by combining X-ray diffraction, neutron diffraction, and DFT calculations. Importantly, a reversible structural isomerization was found to occur on this Au22(SR)15H. By combining the crystal structures and theoretical calculations, the focus was placed on the hydride-binding site, and a [Au–H] migration mechanism of this isomerization process is clearly shown. Furthermore, this [Au–H] migration mechanism is confirmed by the subsequent capture and structural determination of theoretically predicted intermediates. This work provides insight into the dynamic behavior of hydride ligands in nanoclusters and a strategy to study the evolution mechanism of nanoclusters by taking the hydride ligand as the breakthrough point.
研究领域:
材料科学
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纳米技术
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物理化学
·
理论化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Research
·
2020
·Vol. 13
·期 10
·2706-2715
·
IF 8.183
Yan Jing,Zhu Rongying,Wu Fan,Zhao Ziyin,Ye Huan,Hou Mengying,Liu Yong,Yin Lichen
Insufficient intratumoral penetration greatly hurdles the anticancer performance of nanomedicine. To realize highly efficient tumor penetration in a precisely and spatiotemporally controlled manner, far-red light-responsive nanoclusters (NCs) capable of size shrinkage and charge conversion were developed and co-administered with iRGD to synergistically improve the intratumoral penetration and the anticancer efficacy. The NCs were constructed using the singlet oxygen-sensitive (SOS) polyethylene glycolpolyurethane-polyethylene glycol (PEG-( 1 O 2 )PU-PEG) triblock copolymer to encapsulate the doxorubicin (DOX)-loaded, chlorin e6 (Ce6)-conjugated polyamindoamine (PAMAM) dendrimer (DCD) via the double-emulsion method. Co-administration of iRGD notably increased the permeability of NCs within tumor vasculature and tumor tissues. In addition, upon far-red light irradiation (660 nm) of tumors at low optical density (10 mW/cm 2 ), the generated 1 O 2 could disintegrate the NCs and release the DCD with positive surface charge and ultra-small size (∼5 nm), which synergized with iRGD to enable deep intratumoral penetration. Consequently, the local 1 O 2 at lethal concentrations along with the released DOX efficiently and cooperatively eradicated tumor cells. This study provides a convenient approach to spatiotemporally promote the intratumoral penetration of nanomedicine and mediate programmed anticancer therapy.
研究领域:
药剂学
·
肿瘤学
·
生物材料学
✓ 已验证
DOI
高影响因子
Nano Research
·
2016
·Vol. 9
·期 4
·1182-1192
·
IF 8.893
Li Lixiong,Huang Shuangshuang,Song Jianjun,Yang Nating,Liu Jingwei,Chen Yuyun,Sun Yuhan,Jin Rongchao,Zhu Yan
Ultrasmall Au 10 clusters have a unique electronic structure and can act as a charge reservoir to donate electrons or accept charges. This is particularly important for catalysis, since it leads to facile charge transfer across the interface between the gold species and the oxide substrate. To determine the electronic and structural effects of Au 10 on the catalytic oxidation, a TiO 2 charge carrier was chosen as the substrate to anchor Au 10 for olefin oxidation. Au 10 supported on TiO 2 -RP (RP = pyramid-capped columnar structure) exhibited superior catalytic activity to Au 10 /TiO 2 nanotubes and Au 10 /P25. In addition, the supported Au 10 clusters gave rise to higher activity than supported Au 20 , Au 144 clusters, and 5 nm Au nanocrystals. The superior catalytic ability of Au 10 /TiO 2 -RP arises from the charge/discharge effect of the Au 10 /TiO 2 -RP interface, which effectively improves the formation of active oxygen species on electron-rich gold atoms at the terminal position of Au 10 , and promotes the activation of olefin C=C bonds on the electron-deficient gold atoms of Au 10 .
研究领域:
材料科学
·
催化化学
✓ 已验证
DOI
高影响因子
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
·
2020
·Vol. 146
·期 4
·875-882
·
IF 3.656
Wang Maonan,Wang Lishan,Feng Huan,Jiang Hui,Zhou Jiahua,Wang Xuemei
Purpose Presently, liver cancer is still one of the malignant tumors with high mortality. As far as the treatment of liver cancer is concerned, the most effective method is still liver transplantation. But every year, many liver cancer patients die from the lack of a proper liver transplant, or from waiting for a liver transplant. Therefore, it is very important to find new and effective treatment for patients with liver cancer. Methods Herein, the cell model and the orthotropic liver tumor mice model have been performed to verify the results of our treatment. We found that the in situ synthesized gold nanocluster–PTEN (GNC–PTEN) complexes can effectively target and realize the fluorescence imaging of the liver tumor. Results GNC–PTEN complexes could inhibit the proliferation, invasion, and metastasis of liver cancer cells. And the results also showed that GNC–PTEN complexes could be well targeted liver tumor at 6 h and the liver tumor in mice group treated with GNC–PTEN complexes almost disappeared. Conclusion This is a simply and effectively method to realize liver cancer imaging and inhibition. This may raise the possibility for the accurate image/diagnosis and simultaneously efficient treatment of liver cancer in the relevant clinic application.
研究领域:
肿瘤学
·
分子生物学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）