MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 12
·1-8
·
IF 5.479
Hou Guorong,Yun Yanjing,Wang Minqiang,Wang Ying,Chen Hao,Zhang Longcheng,Wang Feng,Xia Qingyou,Liu Yang,Lu Zhisong,Bao Shu-Juan
A corn-like CeO 2 /C coaxial cable textured by a cerium oxide shell and a carbon core was designed to sense NO. The carbon core possesses high electrical conductivity, and the CeO 2 surface delivers excellent electrocatalytic activity. The sensor, typically operated at 0.8 V (vs. Ag/AgCl), exhibits a detection limit of 1.7 nM, which is 4-times lower than that of CeO 2 nanotubes based one (at S/ N = 3). It also displays wide linear response (up to 83 μM), a sensitivity of 0.81 μA μM −1 cm −2 , and fast response (2 s). These values are highly competitive to that of a CeO 2 tube (0.92 μA μM −1 cm −2 and 2 s). The sensor was used to quantify NO that is released by Aspergillus flavus.
Schematic representation of corn-like CeO 2 /C which can more sensitively and effectively detect NO released from A. flavus than when using CeO 2 nanotubes, benefitting from its unique coaxial cable structure.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 2
·1-9
·
IF 5.479
Chen Jia,Li Yunchun,Huang Yanni,Zhang Haijuan,Chen Xingguo,Qiu Hongdeng
The authors describe a fluorometric strategy for the determination of dopamine (DA). It is based on the use of aptamer-functionalized MoS 2 quantum dots (QDs) and MoS 2 nanosheets (NSs). The QDs and NSs were extensively characterized with regard to their physical and chemical properties using methods such as TEM, XRD, FT-IR, EDX and molecular spectroscopies. The aptamer against dopamine was labeled with QDs acting as the energy donor in an energy transfer system, while the NSs serve as the energy acceptor. Under the optimal conditions, the fluorescence (FL) intensity (best measured at excitation/emission peaks of 315/412 nm) increases with increasing DA concentration in the range from 0.1 nM to 1000 nM, with a lower detection limit of 45 pM. The method was successfully applied to the determination of DA in complex matrices. In our perception, the method has a wide scope in that it may be extended to other biomolecules for which respective aptamer are available. The QDs show excellent optical properties, good stability, low cytotoxicity, and may also be applied to fluorometric imaging of live cells.
A “turn-on” fluorometric aptasensor for the determination of dopamine (DA) was established based on aptamer-functionalized molybdenum disulfide quantum dots (MoS 2 QDs) and MoS 2 nanosheets. This assay exhibits high selectivity and sensitivity with a detection limit as low as 45 pM.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 9
·1-9
·
IF 5.705
Wang Zhong-Xia,Gao Yuan-Fei,Yu Xian-He,Kong Fen-Ying,Lv Wei-Xin,Wang Wei
The authors describe the synthesis of fluorescent coral-like carbon nano-branched polymers (PCNBPs) co-doped with nitrogen and phosphorus. Uric acid and phosphoric acid act as nitrogen and phosphorus sources, respectively. The PCNBPs have a coral-like branched structure, are cross-connected, and < 20 nm in skeleton diameter. Their blue fluorescence, best measured at excitation/emission wavelengths of 330/425 nm, is quenched by mercury (II) ions due to the specifically restricted rigid conformation caused by the interaction of phosphorus, nitrogen, and oxygen groups on the surface of the PCNBPs. Fluorescence is selectivity quenched by Hg(II) but restored in addition of the hypertension drug captopril (CAP) in the range 50 nM to 40 μM concentration range. Fluorescence recovery is attributed to the effectively specific interactions between the thiol group of CAP and Hg(II). The method was applied to the determination of the concentration of Cap in pharmaceutical samples, and recoveries were between 97.6 and 105.1%.
Fluorescent coral-like carbon nano-branched polymers (PCNBPs) co-doped with nitrogen and phosphorus are described. Their fluorescence is selectivity quenched by Hg(II) but restored in addition of the hypertension drug captopril (Cap) in the range 50 nM to 40 μM concentration range.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 4
·1-8
·
IF 5.705
Liu Xinnan,Zhang Wentao,Huang Lunjie,Hu Na,Liu Wei,Liu Yingnan,Li Sihang,Yang Chengyuan,Suo Yourui,Wang Jianlong
A method is described for the rapid fluorometric determination of dopamine (DA) by using molybdenum disulfide quantum dots (MoS 2 QDs) that were fabricated via an ammonium hydroxide etching method. The probe has a fluorescence (with excitation/emission peaks at 267/380 nm) that is quenched by DA with high selectivity over various interferences. This is attributed to a reaction that occurs between DA and the molybdate ions in pH 9 solutions of MoS 2 QDs. The formation of organic molybdate complexes and of dopamine-quinone results in strong quenching of the fluorescence of the QDs which is due to both electron transfer and an inner filter effect. Under the optimum conditions, the assay works in the 0.1–100 μM DA concentration range, with two linear ranges and a 10 nM detection limit. The method was applied to the determination of DA in spiked artificial urine samples, where it gave recoveries ranging from 97.6 to 102.2%, demonstrating that the method a promising tool for rapid and selective detection of DA.
MoS 2 QDs are facilely synthesized via the etching effect of ammonium hydroxide for highly selective fluorometric detection of dopamine.
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Food Chemistry-X
·
2024
·
IF 6.5
Xueli Wang,Xueting Liu,Chunmei Sun,Yanwei Cheng,Zhen Li,Shuyi Qiu,Yongguang Huang
Daocai is a traditional salted pickle in the southeastern region of Guizhou with a unique aroma, color, and taste. The quality of Daocai is greatly influenced by the fermentation temperature. In this study, high-throughput sequencing and headspace-gas chromatography-ion mobility spectrometry were used to investigate the changes in microbial community succession and volatile flavor compounds during Daocai fermentation under temperature-controlled (D group) and non-temperature-controlled (C group).We found that the predominant genera in the C group samples were Latilactobacillus (40.57 %), Leuconostoc (21.25 %), Cystofilobasidium (22.12 %), Vishniacozyma (23.89 %), and Leucosporidium (24.95 %), whereas Weissella (29.39 %), Lactiplantibacillus (45.61 %), Mucor (68.26 %), and Saccharomyces (23.94 %) were the predominant genera in the D group. A total of 92 VFCs were detected in Daocai samples, including 5 isothiocyanates, 16 esters, 14 alcohols, 24 aldehydes, 17 ketones, 3 acids, 2 pyrazines, 1 pyridines, 1 thiazoles, 3 furans, 4 alkenes, and 2 nitriles. Further analysis revealed Latilactobacillus , Leuconostoc , Lactococcus , Cystofilobasidium , Leucosporidium , Holtermanniella , and Dioszegia as key bacteria involved in flavor formation. They are closely related to the formation of flavors such as aldehydes, furans, pyridines, and alkenes. This study contributes to our understanding of the relationship between bacterial communities and the flavor formation during Daocai fermentation.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2024
·
IF 5.4
Qingling Nie,Peipei Xu,Jiajia Liu,Yanhanzhuo Wang,Qian Huang,Ruiyun You,Yunzhen Liu,Lizhi Li,Yudong Lu
To improve the sensitivity, accuracy and specificity of the assay, a two-dimensional silver substrate with EF=5.85×10 8 was first synthesized as a SERS substrate, on the surface of which DSP molecules were modified to form a DSP-antibody coupling through the activation of two N-hydroxy succinimide (NHS) esters to capture TNF-α. Subsequently, aptamerized silver-coated gold nanospheres (Au@TFMBA@Ag) were synthesized as Surface-Enhanced Raman Scattering (SERS) recognition probes. These probes were employed to create a sandwich structure for the quantitative detection of Tumor Necrosis Factor-alpha (TNF-α), utilizing the SERS signal intensity at 1374 cm −1 . Quantitative detection of TNF-α was successfully accomplished within the concentration range of 10 −4 to 10 −10 mg·mL −1 . Clinical serum samples were collected and subjected to testing. Significance analysis, conducted through the T-test (p < 0.0001), unequivocally showed the method's ability to differentiate between sera from normal individuals and those diagnosed with colon cancer.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Yanan Liu,Jiacheng Gan,Fei Chen,Jian Chen,Huan Li,Yanbo Wang
Dual-enzyme co-embedded materials have shown high potential for achieving efficient detection due to the convenience of two-enzyme cascade reactions. Herein, we developed a dual-enzyme hybrid microsphere (HM) based biosensor to detect diamines (histamine was included for ease of description) in aquatic products. The HM was made from diamine oxidase , horseradish peroxidase , and copper phosphate through the biomineralization method. Under optimal conditions, the system displayed linear color response to histamine of different concentrations ranging from 0 to 200 μg/mL. The detection limit of histamine was 0.15 μg/mL, showing higher sensitivity than the two-step free enzyme assay . Moreover, the detection system exhibited good specificity to diamines. The method was used to detect diamines in commercial samples, and the results were compared with those measured by the high-performance liquid chromatography method. Overall, the proposed assay exhibited high potential in diamine quantification and was readily extended to other cascade enzymatic reaction-based detection strategies.
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SCIENCE OF THE TOTAL ENVIRONMENT
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2024
·
IF 8.2
Lian Xia,Feng Luo,Xiaojuan Niu,Yue Tang,Yuangen Wu
Pb(II) is a prevalent heavy metal ion classified as a 2B carcinogen. Excessive intake of Pb(II) in the human body can damage the central nervous system, kidneys, liver, and immune system, leading to permanent brain damage, anemia, and cancer. Colorimetry can be applied to rapidly determine Pb(II) residues, but there are still many challenges in the accuracy and sensitivity of detection. Based on the inhibitory impact of Pb(II) on the oxidase-like activity of octahedral silver oxide (Ag 2 O), a colorimetric sensor with smartphone-assisted analysis for the Pb(II) detection was first developed. Herein, it has been found that Pb(II) can adsorb onto the surface of octahedral Ag 2 O, hindering the production of O 2 − in the reaction system. This ultimately results in the suppression of oxidase-like activity, leading to a lighter purple appearance of the colorimetric reaction solution. The sensor exhibits a high degree of sensitivity and a limit of detection (LOD) for Pb(II) was calculated as 2.2 μg L −1 . Hence, the developed colorimetric sensor with high sensitivity, excellent specificity, and high tolerance to sodium ions is hopeful to have practical applications in Pb(II) detection in environmental water samples. Moreover, the sensor will provide a novel strategy for heavy metal ion detection and other substances.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Shen Zhang,Xiao Wang,Yutong Wang,Jiaxu Dong,Yixuan Zhang,Manlin Zhao
Vitamin B12 is a drug that can participate in the production of bone marrow red blood cells, prevention of pernicious anemia and damage to brain nerves. It is needful to prepare new vitamin B12 determination strategies with excellent sensitivity, selectivity and uniqueness. Herein, a novel and facile chemical reduction method was successfully carried out (histidine and ascorbic acid were applied as protectant and reductant) for preparing histidine-functionalized silver nanoclusters (Ag NCs@his) with blue fluorescence. Some technologies were adopted to clarify the structure, optical characteristics of this stable Ag NCs@his and fluorescence quenching mechanism. Based on inner filter effect, a sensitive and selective platform for vitamin B12 detection was established. Under optimum pH value, reaction time and temperature, the as-developed platform could be utilized to the trace measurement of vitamin B12 with linear range of 0.5–200 μM and detection limit of 0.038 μM. Moreover, this platform was successfully used to measure vitamin B12 in real samples and temperature.
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JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2025
·Vol. 2025
·期 1
·6298724
·
IF 2.5
Niuniu Bai,Jianlin He,Bihong Hong,Zelong Wu,Liping Wang,Zongze Shao
Background: Traditional fish sauce is made from fish that have been coated in 30%–40% sea salt and fermented for up to 2 years. The preparation requires a long time, and the fish source contains considerable biogenic amines. In this report, by measuring the growth curve and identifying the intracellular enzyme and the activity of protease, B. zhangzhouensis with the highest activity of protease production was selected from eight strains, which were applied to the fermentation process of fish sauce. Results: Optimized by response surface methodology, the content of amino nitrogen was taken as the response value, and the best fermentation conditions were 4.15% inocula, pH 9.13, and 36.3°C. Under the optimized conditions, fish sauce samples were obtained by fermentation with B. zhangzhouensis for 15 h, 3 days, 7 days, 15 days, and 30 days, respectively. The contents of amino nitrogen, total nitrogen, biogenic amine, and various flavor substances were determined and compared with the traditional 2-year-fermented fish sauce. As a result, the 7-day-fermented fish sauce using B. zhangzhouensis had a similar composition of flavor substances to traditional fish sauce, but a much lower level of biogenic amines. Conclusion: The new method of preparing fish sauce with a fermentation condition of 4.15% B. zhangzhouensis , pH 9.13, and 36.3°C can improve the production efficiency of fish sauce, and the flavor is similar to that of traditional fish sauce.
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TALANTA
·
2025
·
IF 6.1
Yifei Ma,Lijie Zhang,Hong Yang,Shanshan Zhu,Jinhua Liu
Highly selective and sensitive determination of dopamine (DA) from multiple sources remains a persistent and significant challenge. Here, we develop an imidazole-triggered in situ fluorescence reaction system for highly selective and sensitive determination of DA from various sources (human, horse, dog, rabbit, and mouse). The system operates by catalyzing the oxidation of DA with 1,5-Dihydroxynaphthalene (1,5-DHA) through a Lewis base formed by imidazole, leading to the rapid generation of yellow azamonardine fluorescent compounds (AFC). Notably, the system demonstrates minimal background noise and a high signal-to-noise ratio of up to 300-fold with a determination limit of 33.33 pM, making it 10-100 times more sensitive than conventional enzyme-linked immunosorbent assay (ELISA) methods. Moreover, selectivity tests reveal that our system can effectively distinguish between several common interfering substances, even at concentrations as low as 10 nM. The developed system shows promising results in detecting DA from diverse sources (humans, horses, dogs, rabbits, and mice), including urine samples from clinical patients, exhibiting good agreement with traditional ELISA kits. Therefore, the established in situ fluorescence reaction system holds great potential for the determination of DA-related disorders due to its impressive analytical capabilities.
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FOOD CONTROL
·
2024
·
IF 5.6
Xiaohan Hua,Tingting Yan,Shuyi Liu,Lijun Yin,Xin Jia
The high protein content in sufu provides a substrate for microorganisms to produce biogenic amines (BAs), thereby posing a potential risk to food safety. The aim of this study was to identify the main BAs produced by different types of sufu (red, white and grey sufu) and investigate the relationship between bacterial diversity and BA production using high-throughput sequencing. The results revealed significant differences in the total BA content of the three types of sufu at the post-fermentation stage, with grey sufu exhibiting the highest total BA content (ranging from 6389.00 mg/kg to 7255.49 mg/kg). The main BAs found in all three types of sufu were tyramine, putrescine and cadaverine. Enterococcus genus produced high levels of tyramine and phenylethylamine in all three types of sufu. The predominant cadaverine-producing bacteria in red sufu was Enterobacter genus, whereas Klebsiella pneumoniae was the primary producer of cadaverine in white and grey sufu. Putrescine was primarily associated with the Bacillus and Enterobacter genera in red and grey sufu. Acinetobacter , Enterococcus and Enterobacter were the common dominant genera during the post-fermentation stage of the three kinds of sufu, which were positively correlated with the production of various BAs. These findings will help to understand the mechanism of BAs production by bacterial communities in different types of sufu to ensure safe production.
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