ACS Applied Materials & Interfaces
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2023
·
IF 9.5
Xueping Quan,Bing Yan
Metal-organic framework (MOF) and covalent-organic framework (COF) hybrid materials can combine the unique properties of MOF and COF components, and their applications in fluorescence sensing have attracted more and more attention. Herein, ZIF-90 is grown on 3D-COF by a simple in situ growing method in which the 7-amino-4-methylcoumarin (AMC) is encapsulated in ZIF-90 to construct a fluorescent sensor. Chloroquine phosphate (CQP) can coordinate with Zn2+ to decompose the ZIF-90 and release AMC. At 365 nm excitation, the ratiometric fluorescence signal AMC/3D-COF (I430/I598) increases linearly with CQP in a linear range of 4 × 10–5 to 4 × 10–4 M in urine. Under 340 nm excitation, quantitative analysis of CQP in the serum (3 × 10–6 to 4 × 10–5 M) is based on the fluorescence intensity of Zn-CQP/3D-COF (I384/I598). In addition, AMC@ZIF-90/3D-COF (1) exhibits high anti-interference and selectivity in sensing of FA with a “turn off” mode, with a correlation range of 1 × 10–5 to 1 × 10–3 M. The fluorescence color changes triggered by CQP under different excitation conditions, and the different fluorescence responses caused by CQP make it a highly secure anticounterfeiting platform. The synthesized dye@MOF/COF hybrids not only provide a new way to integrate multiple emission to design fluorescent probes for differentiation detection but also offer ideas for the design of anticounterfeiting platforms.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
·
IF 6.1
Wei Jia,Jiying Zhu
ε-Polylysine is a novel food preservative approved by the U.S. Food and Drug Administration (FDA), yet the mechanism of its effect on animal-derived foods remains unclear. Assessment of the effect of preservatives on goat meat products is necessary. Herein, metabolite accumulation and protein expression of ε-polylysine (0.025%, w/w) spiked with goat meat were investigated by nontarget metabolomics and proteomics combined with ultrahigh performance liquid chromatography quadrupole-Orbitrap high-resolution-mass spectrometry (UHPLC-Q-Orbitrap HRMS) in a simulated in vitro digestion model. The amino side chain of ε-polylysine increased the activity of glycine aminotransferase due to its nucleophilic nature, inducing a significant upregulation of l-arginine (0.43–0.72 mg kg–1) and creatine (3.98–6.89 mg kg–1), with an improvement in muscle quality of goat meat. Downregulation of enzyme phenylalanine hydroxylase expression led to upregulation of l-phenylalanine (2.26–3.25 mg kg–1) and l-tyrosine (0.98–1.29 mg kg–1). Collectively, this study first revealed the biochemical mechanism of ε-polylysine in goat meat products, which makes available new prospects for more accurate use of ε-polylysine in animal-derived foods.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 50
·109110-109122
·
IF 5.8
Liu Jiadi,Dong Yawen,Kang Yu,Kong Qian,Wang Kuaibing,Mao Feifei,Bu Yuanqing,Zhou Rong,Zhang Chunyong,Wu Hua
Developing multifunctional catalysts applied in diversiform modes via advanced oxidation processes (AOPs) is a promising and attractive approach for organic pollution degradation. Herein, a novel hollow bamboo-like structural cobalt/nitrogen-doped carbonized material (CoC/N) was employed as a catalyst for AOPs, in which CoC/N was prepared in situ through calcining a Co-based coordination polymer. When CoC/N was utilized as a peroxymonosulfate (PMS) activator, the catalyst stood out prominent activities for effective CA oxidation. Furthermore, a five-level central composite rotatable design (CCRD) model describing CA decay as a function of PMS concentration, CoC/N dosage, and solution pH value was successfully constructed and engaged to explore the optimal operating conditions. Finally, the possible degradation mechanism of CA in CoC/N-PMS system was proposed by quantum chemistry calculation and LC/MS analysis. This work shed light on the structural morphology of the catalyst and its PMS synergy degradation pathway, which promotes its applications in miscellaneous pollutant degradation. Graphical Abstract A new Co/N-doped material was used to degrade unconventionality organic pollutant creatinine (CA) for the first time, in which the scientific approaches of five-level central composite rotatable design (CCRD) model, response surface methodology (RSM) and density function theory (DFT) were employed to evaluate the material performance and CA degradation pathway. The toxicity evaluation, statistical modeling and mechanisms study have been investigated meticulously
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ACS Biomaterials Science & Engineering
·
2023
·
IF 5.8
Kaibo Xu,Liqin Cao,Zhouyu Wang,Liu-ping Chen
Hemoperfusion (HP) is one of the most prominent therapies for treating uremia, hyperbilirubinemia, and acute drug toxicity. The comprehensive performance of currently used porous HP adsorbents needs to be improved due to the impediment to their synthesis strategy. Herein, green carbon dioxide-in-water high internal phase emulsions (C/W HIPEs) were utilized and emulsified with poly(vinyl alcohol) (PVA) for the formation of a heparin-mimetic chitosan oligosaccharides/poly(acrylamide-co-sodium 4-styrenesulfonate) [COS/P(AM-co-SSS)] monolith, which exhibited good mechanical properties, stable swelling performance, hydrophilic properties, anticoagulant effect, and low hemolysis. It showed a strong toxin adsorption capacity (415.2 mg/g for creatinine, 199.3 mg/g for urea, 279.5 mg/g for bilirubin, and 160 mg/g for tetracycline). The adsorption process of porous COS/P(AM-co-SSS) followed the pseudo-second-order kinetic and Langmuir isotherm models. Moreover, the porous materials had a strong electrostatic force on creatinine. The removal of creatinine by simulated in vitro blood perfusion was 80.2% within 30 min. This work provides a green preparation strategy for developing novel HP materials, highlighting their potential application value in blood and environmental purification.
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TALANTA
·
2023
·
IF 6.1
Jingyu Xiao,Yanxia Chen,Tailin Xu,Xueji Zhang
Urinalysis is an effective strategy to non-invasively evaluate human health, and surface-enhanced Raman scattering (SERS) may be a powerful technique for use in detecting analytes in urine. Herein, we report a wearable diaper sensor based on a handheld Raman spectrometer for use in the simple, label-free identification of biomolecules (urea, creatinine, and bilirubin) in urine. The raspberry-shaped Au substrate formed on the surface of an Si wafer provides plasmonic enhancement of the SERS signals, with an excellent uniformity and stability. The SERS sensor combines the advantages of flexibility, portability, and multifunctional detection and may be used in identifying multiple analytes in urine. The sensor exhibits high sensitivities in detecting urea, creatinine, and bilirubin , with respective detection limits of 4.17 × 10 −3 M, 5.90 × 10 −6 M, and 1.38 × 10 −7 M (signal-to-noise ratio = 3). Furthermore, we used the wearable diaper sensor to monitor biomolecules at the diagnostic threshold, facilitating non-invasive diagnosis and medical monitoring of disease-related biomarkers.
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ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Chunfang Wang,Yun Tang,Bihong Zhang,Ziying Zhong,Faqiong Zhao,Baizhao Zeng
In this study, PO 4 3− doped Bi 2 WO 6 (BWO-PO) was prepared by hydrothermal method , and then copolymer of thiophene and thiophene-3-acetic acid (P(Th-T3A)) was chemically deposited on the BWO-PO surface. The introduction of PO 4 3− created point defects , greatly improving the photoelectric catalytic performance of Bi 2 WO 6 ; the copolymer semiconductor could form heterojunction with Bi 2 WO 6 to promote the separation of photo-generated carriers, due to its proper band gap. Furthermore, the copolymer could enhance the light absorption ability and photo-electronic conversion efficiency. Hence, the composite had good photoelectrochemical properties. When it was combined with carcinoembryonic antibody through the interaction of –COOH groups of the copolymer and the end groups of antibody for constructing ITO-based PEC immunosensor, the resulting sensor exhibited superb response to carcinoembryonic antigen (CEA), with a wide linear range of 1 pg/mL−20 ng/mL, and a relatively low detection limit of 0.41 pg/mL. It also showed high anti-interference ability, stability, and simplicity. The sensor has been successfully applied to monitor the concentration of CEA in serum. The sensing strategy can also be applied to the detection of other markers by changing the recognition elements, hence it has good application potential.
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Arabian Journal of Chemistry
·
2023
·
IF 6
Bushra Rafique,Rizwan Ullah Khan,Aysha Sarfraz Rizvi,Muhammad Irfan,Ghulam Murtaza,Lili Qiu,Min Xue,Zihui Meng
There is a demand for simple, selective, and efficient assays for the determination of clinically important metabolites such as creatinine for healthcare. Creatinine is the by-product of muscle energy metabolism and is excreted by the kidneys. To measure creatinine in the human serum, a creatinine imprinted photonic crystal hydrogel (CIPC hydrogel) for naked-eye detection is developed. CIPC hydrogel utilizes polystyrene-based two-dimensional (2D) photonic crystal colloidal arrays (PCs-array) embedded in the polyacrylamide hydrogel containing methacrylic acid which imprinted the creatinine template. The nanocavities in the hydrogel produced after the removal of the template bind to and recognize creatinine in the serum samples. The binding is selective and specific for creatinine. The binding is observed as shrinkage of the hydrogel volume and a decrease in the particle spacing which is monitored through changes in the Debye diffraction ring diameter and a visible blue-green to blue color shift. The binding event and the mechanism are investigated by molecular dockings. The CIPC sensor demonstrates a limit of detection (LOD) of 2.45 ± 1.6 µM, a linear detection range (25–500 µM), and recovery from 85.6 % to 99.9 % in the serum samples. CIPC hydrogel is available for the rapid and quantitative onsite detection of creatinine in the human serum sample.
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JOURNAL OF ETHNOPHARMACOLOGY
·
2023
·
IF 5.4
Xu Zhang,Xiaotong Wang,Rui Shi,Xiaoku Ran,Xiahong He,Deqiang Dou
Ethnopharmacological relevance Red ginseng (RG), a processed product of ginseng (GS), is a generally used qi -tonifying medicine in Traditional Chinese Medicine (TCM). According to the TCM principle, RG is also generally applied to spleen-deficiency syndrome (SDS) clinically for its warmer property. However, the effective substances and mechanism of RG on SDS have not been well investigated. Aim of the study The aim of this study was to explore the effective substances and their mechanism of RG on SDS. Materials and methods The SDS model was established with a compound factor method involving an irregular diet, excessive fatigue and sennae folium with a bitter-cold property. The medicine of RG was split by multi-mode separation methods and analyzed by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS). The appearance indexes such as body weight, body temperature, swimming endurance, urine output, and water content of fecal were determined. The biochemical indexes such as D-xylose, SP , VIP and AChE in the digestive system, CRH, ACTH, CORT, E, T3, T4, T, E2 and 5-HT in the endocrine system, CS, NCR , IDH1 , COX and Na + -K + -ATPase in the metabolism of substance and energy, cAMP and cGMP in the cyclic nucleotide system were analyzed by Enzyme-linked immunosorbent assay (ELISA) kits and biochemical kits. The serum metabolites were analyzed by UPLC-QTOF/MS. Furthermore, the gut microbiota and short-chain fatty acids (SCFAs) in feces were analyzed by 16S rRNA sequencing and headspace gas chromatography-mass method. Results The pharmacological experiments showed that total saponin fraction (RGTSF), less polar fraction (RGLPF), and polysaccharides faction (RGPSF) significantly modulated the “brain-gut” axis-related indexes (the levels of VIP, AChE, and 5-HT). Besides, RGTSF also significantly modulated the hypothalamic-pituitary-adrenal (HPA) axis-related indexes as well as the substance and energy metabolism-related indexes (the levels of ACTH, CORT, A, Na + -K + -ATPase, COX, NCR and CS). RGPSF also significantly modulated the hypothalamus-pituitary-thyroid (HPT) axis-related indexes (the levels of T3 and T4). Secondly, metabolomics indicated that RGTSF could significantly regulate the abnormal metabolic pathways associated with the development of SDS, which involved steroid hormone biosynthesis, taurine and hypotaurine metabolism, primary bile acid biosynthesis, and amino acid metabolism. Subsequently, the study of gut microbiota indicated that RGLPF could increase the diversities of the gut microbiota and the relative abundance of Firmicutes in rats with SDS, while RGWEF significantly increased the relative abundance of Bacteroidetes . At the genus level, RGLPF could increase the relative abundance of Lactobacillus in rats with SDS and decrease that of Akkermansia . Meanwhile, the water-eluted fraction (RGWEF) showed a stronger regulation in SCFAs. Conclusion It is for the first time that the effective substances of red ginseng on spleen-deficiency syndrome were studied systematically, and the different mechanisms of the RG fractions involved in substance and energy metabolism as well as the “brain-gut” axis were revealed. The present study demonstrated that RGTSF, RGPSF, and RGLPF were the effective substances of red ginseng for ameliorating spleen-deficiency syndrome, indicating that ginsenosides composed of primary and secondary saponins as well as polysaccharides were the main effective substances for red ginseng in ameliorating spleen-deficiency syndrome.
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CELLULOSE
·
2023
·Vol. 30
·期 8
·5187-5200
·
IF 5.7
Li Jie,Lu Dechan,Yang Jinglei,You Ruiyun,Chen Jingbo,Weng Jingzheng,Lu Yudong
We developed a method for the fabrication of in-situ reduced bacterial cellulose membrane (BCM) for the simple synthesis of three-dimensional (3D) flexible SERS substrate. Ag NPs with high density were in-situ grown and uniformly distributed on the 3D BCM nanofibers through a controlled “silver mirror reaction” and “volume shrinkage” treatment, which created highly stable and uniform hot spots in the networked SERS substrate. “Hydrogel” BCM was formed by soaking in ammonia solution to hydrolyze the hemiacetal structure of cellulose, followed by in-situ reduction in silver trifluoroacetate (CF 3 COOAg) solution, the BCM enriched with silver nanospheres (Ag NPs-BCM) substrate was prepared. The results showed that the optimized Ag NPs-BCM substrate exhibited high SERS activity, and displayed an average EF of 5.3 × 10 8 and RSD of 11.0%, especially when protected from light for 45 days, the SERS performance kept steady. The Ag NPs-BCM substrate was used to detect glutathione (GSH). Interestingly, adding Ellman's reagent (DTNB) to serum, we clearly distinguished the amount of GSH content in cancer and normal using Ag NPs-BCM substrate. This has provided the opportunity to use flexible substrate in rapid biomedical assays.
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2023
·Vol. 140
·期 19
·e53895
·
IF 3
Xingtong Gao,Kai Liu,Peng Liu,Xiaoyu Bai,Aixiang Li,Zijian Lyu,Qiuhong Li
In this paper, acrylic acid (AA) and sodium styrene sulfonate (SSS) were grafted onto the surface of cellulose acetate (CA) by free radical polymerization to obtain a heparin unit-containing graft copolymer CA-g-P(AA/SSS). It was used to coat the adsorbent resin for disposable hemoperfusion devices to improve blood compatibility. The adsorption capacity of the modified CA-coated adsorbent resin to creatinine, Vitamin B12, bilirubin, and imidacloprid was tested. The results showed that the modified CA-coated adsorbent resin had a good adsorption effect on small and medium-sized molecules. The hemolysis and coagulation tests showed that the CA-g-P(AA/SSS) coating could greatly improve the blood compatibility of the adsorbent resin. The hemolysis rate was 1.6%, which met the requirement of international standards for the hemolysis rate of blood contact equipment less than 5%. The coagulation index was higher than 1.55, which was comparable to the heparin-preflashed HA130 currently in use. In addition, the CA-g-P(AA/SSS)-coated adsorbent resin has good biocompatibility, no pyrogenic reaction, low vitro cytotoxicity, and no acute systemic toxicity. The resin has a good application prospect in uremia, hyperbilirubinemia, and rapid treatment of pesticide poisoning.
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Advanced Science
·
2023
·Vol. 10
·期 15
·2206494
·
IF 15.1
Shaowei Xie,Xiaochen Fei,Jiayi Wang,Yi-Cheng Zhu,Jiazhou Liu,Xinxing Du,Xuesong Liu,Liang Dong,Yinjie Zhu,Jiahua Pan,Baijun Dong,Jianjun Sha,Yu Luo,Wenshe Sun,Wei Xue
High-throughput metabolic fingerprinting has been deemed one of the most promising strategies for addressing the high false positive rate of prostate cancer (PCa) diagnosis in the prostate-specific antigen (PSA) gray zone. However, the current metabolic fingerprinting remains challenging in achieving high-precision metabolite detection in complex biological samples (e.g., serum and urine). Herein, a novel self-assembly MoS 2 /MXene heterostructure nanocomposite with a tailored doping ratio of 10% is presented as a matrix for laser desorption ionization mass spectrometry analysis in clinical biosamples. Notably, owing to the two-dimensional architecture and doping effect, MoS 2 /MXene demonstrates favorable laser desorption ionization performance with low adsorption energy, which is evidenced by efficient urinary metabolic fingerprinting with an enhanced area under curve (AUC) diagnosis capability of 0.959 relative to that of serum metabolic fingerprinting (AUC = 0.902) for the diagnosis of PCa in the PSA gray zone. Thus, this MoS 2 /MXene heterostructure is anticipated to offer a novel strategy to precisely and noninvasively diagnose PCa in the PSA gray zone.
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BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Ying Gan,Ning Hu,Chuanjiang He,Shuqi Zhou,Jiawei Tu,Tao Liang,Yuxiang Pan,Dmitry Kirsanov,Andrey Legin,Hao Wan,Ping Wang
Urolithiasis commonly occurs in kidney and ureteral, and may cause local organ/tissue damage, even kidney failure. The incidence of this disease is increasing worldwide, in which calcium oxalate is the major composition forming the urinary calculus. Therefore, to monitor this disease for the prevention and treatment, measuring the oxalate in the urine is of great significance. Here, a rapid and sensitive colorimetric method was developed based on 3,3',5,5'-tetramethylbenzidine-manganese dioxide (TMB-MnO 2 ) nanosheets for oxalate detection. MnO 2 nanosheets acted as an efficient biomimetic oxidase to catalyze the reaction with TMB and oxalate. Pale yellow TMB can be oxidized to blue oxide TMB catalyzed by BSA-stabilized MnO 2 nanosheets, and oxalate can selectively inhibit this reaction by consuming and reacting with MnO 2 nanosheets, thus achieving the quantitative detection of oxalate. Moreover, a home-made bionic electronic-eye (E-eye) system was developed as a portable in-situ detection platform to efficiently measure the oxalate concentrations in 10 s by direct photographing. By optimizing experimental conditions, this method shows a wide linear range (7.8 μM to 250 μM) and a low detection limit (0.91 μM) for oxalate detection. Besides, this method exhibits high selectivity even with 80-fold interfering chemicals. Furthermore, the performance of the method was validated by testing the artificial urine samples, indicating its great potential for monitoring and diagnosis of urolithiasis in point-of-care applications.
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