Aquaculture Reports
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2024
·
IF 3.2
Kangyuan Qu,Siyuan Xiong,Caiqian Huang,Xiaoxia Jiang,Lixia Tian,Shiwei Xie
Ornithine is an arginine family amino acid and is not incorporated into protein. This study evaluated its role in growth, immune response and acute NH 3 stress tolerance of Penaeus vannamei . Six diets with graded levels (0.6, 0.9, 1.2, 1.5, 1.8, 2.1 g kg −1 ) of L-ornithine were formulated, and fed the shrimp for 8 weeks. The growth of shrimp was significantly improved with the dietary ornithine levels increased from 0.6 to 1.5 g kg −1 . Feed efficiency, protein efficiency ratio and protein productive value showed a similar trend to the growth performance of shrimp. Whole body protein and muscle lipid of shrimp were significantly increased in shrimp fed 1.8 g kg −1 ornithine compared to those fed 0.6 g kg −1 ornithine. The contents of several amino acids were increased with the dietary ornithine increased to 1.5 g kg −1 . Elevated levels of ornithine increased the Nitric Oxide (NO) content and suppressed arginase activity in the hemolymph. The relative mRNA expression of superoxide dismutase , heat shock protein 70 , dorsal and caspase 3 were increased with the dietary addition of ornithine, while the mRNA level of arginase was decreased with the increasing dietary ornithine levels. After 9 h acute NH 3 stress, mortality of shrimp reduced with the increasing dietary ornithine levels. The relative mRNA levels of superoxide dismutase , heat shock protein 70 , dorsal and catalase were increased with the dietary addition of ornithine after the acute NH 3 stress. The second-order regression analysis of weight gain and survival after acute stress revealed that the suitable ornithine content in the shrimp diet was 1.4–1.5 g kg −1 .
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APPLIED ORGANOMETALLIC CHEMISTRY
·
2025
·Vol. 39
·期 2
·e7955
·
IF 0
Xiufeng Yang,Rui Chen
Zinc, an important metal element in the human body, fulfills multiple biological roles, such as influence on cell division and differentiation. In this paper, a Maca polysaccharide-zinc (MPS-Zn) complex was prepared by water extraction and alcohol precipitation process. A granular MPS-Zn complex with 0.2360 μg/mg Zn was obtained at 60°C and pH = 3.0 for 60 min. The antioxidant activities of this MPS-Zn complex were evaluated by the free radical scavenging activity toward DPPH and ABTS. The rate of ABTS free radical scavenging of 4.0 mg/mL MPS-Zn complex is equal to that of control group (vitamin C). The abilities of this complex against three cancer cell lines including breast cancer MCF-7, colon cancer SW480, and liver cancer SMMC-7721 were used to evaluate its antiproliferative activity by MTS assay. The results demonstrate that this complex has an inhibitory effect on the above cancer cell lines. Especially, the inhibitory effect of it on colon cancer SW480 is more effective than that of cisplatin. The toxicity of this complex on human normal pulmonary epithelial cell BEAS-2B is lower than that of taxol and cisplatin. This complex has the potential use as the zinc-containing nutritional food and/or anticancer drug.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Shuai Chen,Liang Wang,Shuang Rong,Yuyuan Duan,Huizhu Wang
This study aimed to optimize the extraction process of polysaccharides from Rosa laevigata Michx. (RLMP) using an ultrasound-microwave-assisted method and investigate its in vitro hypoglycemic activity. Key factors affecting RLMP yield were identified using a Plackett–Burman design, followed by a Box–Behnken design and response–surface methodology, to determine the optimal extraction conditions. RLMP was purified using DEAE-52 cellulose, yielding two homogeneous fractions: RLMP-1 and RLMP-2. Monosaccharide composition was analyzed by gas chromatography, and structural characterization of RLMP, RLMP-1, and RLMP-2 was performed using FT-IR, SEM, and TEM. Methylation analysis and NMR were used to elucidate the sugar-chain structure of RLMP-1. In vitro hypoglycemic activity analysis showed that RLMP improved the glucose consumption and glycogen synthesis and enhanced the activities of pyruvate kinase and hexokinase in IR-HepG2 cells. Moreover, RLMP significantly increased the activities of antioxidant enzymes, such as CAT, SOD, and GSH-Px and decreased those of ROS and MDA. Western blotting analysis confirmed that RLMP enhances glucose and lipid metabolism and reduces oxidative stress by activating the PI3K/Akt/GLUT-4 signaling pathway, thereby exerting its hypoglycemic effect. These findings suggest that RLMP is a promising candidate for developing novel antioxidant agents or hypoglycemic drugs.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Pengyue Li,Yongshuai Jing,Xiaoyue Qiu,Huina Xiao,Yuguang Zheng,Lanfang Wu
The purified polysaccharides fraction, DOP-2, was prepared from Dioscorea opposita Thunb ( D. opposita ). This study combined in vitro and in vivo experiments to comprehensively investigate the index changes in RAW264.7 cells and immunocompromised mice under DOP-2 intervention, aiming to elucidate the potential mechanisms of immunomodulatory effects of DOP-2. DOP-2 (10 ∼ 500 μg/mL) significantly elevated the levels of NO, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) factors secreted by RAW264.7 cells, and restored the body weight of immunosuppressed mice and improve the degree of injury to the immune organ index, resulting in significant immunomodulatory effects. Notably, DOP-2 promoted the production of short-chain fatty acids (SCFAs) in immunosuppressed mice and modulated the composition of their gut microflora. These findings highlight the potential benefits of DOP-2 therapy in improving immune function and gut health, and will provide a theoretical basis for the application of D. opposita polysaccharides as an immunomodulatory adjuvant.
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高影响因子
IONICS
·
2025
·
IF 2.6
Du Jinjing,Lin Haiyang,Zhai Ruitong,Liu Xun,Cui Xinxin,Wang Dongbo,Wang Bin,Zhu Jun,Tang Wendan,Zuo Heng,Li Qian,He Xihong
Vanadium redox flow batteries (VRFBs) are considered a highly promising large-scale energy storage technology due to their long lifespan, high safety, large capacity, and high efficiency. In practical applications, pentavalent vanadium in the positive electrolyte of vanadium batteries is prone to precipitation under conditions of high temperatures and concentrations, reducing the energy density and cycle life of VRFBs. Therefore, choosing appropriate additives to improve the stability of pentavalent vanadium ions is crucial. This study researched the effects of two hexahydric alcohols (inositol and galactitol) as electrolyte additives on the performance of vanadium-positive electrolyte through X-ray diffraction (XRD), Raman spectroscopy, cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and charge–discharge tests. The results show that both inositol and galactitol can improve the electrochemical performance of vanadium-positive electrolyte. At a current density of 80 mA cm −2 , the energy efficiency of the battery groups with inositol and galactitol added increased by 1.24% and 3.28% respectively compared to the blank battery group. The capacity retention rates of the battery groups with inositol and galactitol added were both higher than that of the blank battery group after 50 cycles. Due to the different distribution spaces of hydroxyl groups, galactitol was more significant in enhancing electrochemical activity and increasing the stability of the electrolyte.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Chenzhao Wang,Xiujuan Wang,Xinru Gu,Hongmiao Sun,Xinxi Chen,Guoying Sun,Zhibing Wang
Phosphatidylethanolamine (PE) is an important indicator for evaluating the nutritional value and quality of food. In this study, a multimodal detection method (UV/smartphone/fluorescence/paper-based sensing platform) was developed for PE detection in food on the basis of the peroxidase-like activity and fluorescence properties of cerium-doped carbon dots (Ce-CDs). The prepared CDs generated singlet oxygen ( 1 O 2 ) through the Russell mechanism, which oxidized 3,3′,5,5′-tetramethylbenzidine (TMB) to produce ox-TMB. The addition of PE changed the surface charge of Ce-CDs, enhancing their affinity with TMB and causing the solution to turn bluer. In addition, these CDs exhibited excellent optical properties and fluorescence stability. In fluorescence detection, PE significantly quenched the fluorescence of the reaction system through an inner filter effect mechanism. Furthermore, the designed paper-based sensing platform provided a novel tool for on-site high-throughput detection of PE. The signal values of Ce-CDs in various detection modes exhibit a good linear relationship with PE concentrations ranging from 0.1 to 1000 μg/mL, with a lowest detection limit of 0.023 μg/mL. More importantly, all methods successfully realized the detection of PE in real food samples and obtained satisfactory results. This study not only developed multiple detection methods based on the same material but also ensured the accuracy of the results through cross-validation between the different methods.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2025
·
IF 6.3
Hanwen Zhang,Tianxu Wang,Chen Shen,Zhen Wang,Kewei Li,Xian Dang,Mengen Hu,Shuxin Li,Ming Li,Zhulin Huang,Xiaoye Hu
Various hollow structured HfB 2 powders were synthesized via a co-precipitation assisted boron/carbon thermal reduction method, and the influence mechanism of carbon source on morphology was systematically investigated. When glucose or inositol was employed as the carbon source, the hexagonal ring structure formed in the precursor during complexation directed the growth of HfB 2 nanoparticles that were anchored on hollow spherical carbon templates, resulting in a unique hollow core-shell architecture. Additionally, the morphology could be precisely adjusted from hollow spherical to hollow rod-like structures by simply controlling the stirring temperature during complexation. The growth process of HfB 2 with various morphologies was elucidated, and the mechanism of this unique morphological evolution was revealed to originate from the structural differences of precursor complexes. The HfB 2 powders with hollow spherical structure derived from glucose exhibits excellent electromagnetic absorption ability due to the synergistic effect of multiple scattering and interface polarization within the unique hollow structure, with a maximum reflection loss (RL) of −57.8 dB. Quantitative analysis of the loss mechanisms further demonstrates that the outstanding absorption performance is predominantly governed by polarization loss rather than conduction loss.
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Natural Products and Bioprospecting
·
2025
·Vol. 15
·期 1
·66
·
IF 4.9
Xia Xuewei,Lin Jieling,Yang Lihong,Li Youhong,Lin Wenshen,Wang Qingqing,Liu Jun,Huang Riming
Syngnathoides biaculeatus , a potential functional food from marine sources, was found to enhance nonspecific immunity, but its functional ingredients have rarely been reported. Therefore, this study focused on the preparation and physicochemical properties of its water-soluble natural ingredients with their immunomodulatory activities and potential mechanisms. Firstly, we optimized the extraction method of glycoproteins from S. biaculeatus and prepared the crude glycoprotein SYB, from which the fraction glycoprotein SYB-1 was further purified. The carbohydrates of SYB-1 were 8.46% comprising mannose, glucose, and galactose, with an average molecular weight of 9.423 kDa. Amino acid analysis demonstrated that its major amino acids are glycine, glutamic acid, aspartic acid, and proline, with a total amino acid content of 88.81%. Furthermore, SYB-1 could significantly increase the cell viability of macrophages, and promote the release of NO, TNF- α , and IL-6. Metabolomics revealed that it was associated with arachidonic acid metabolism. The CYP450 enzyme family members and PTGS2 may be key targets for the regulatory role. These results suggested that the glycoprotein of S. biaculeatus may be an attractive functional food supplement from natural sources for immunocompromised populations. Graphical
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BIORESOURCE TECHNOLOGY
·
2015
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IF 4.494
Yu Huang,Xiaoyang Wei,Shiguang Zhou,Mingyong Liu,Yuanyuan Tu,Ao Li,Peng Chen,Yanting Wang,Xuewen Zhang,Hongzhong Tai,Liangcai Peng,Tao Xia
In this study, steam explosion pretreatment was performed in cotton stalks, leading to 5–6 folds enhancements on biomass enzymatic saccharification distinctive in Gossypium barbadense and Gossypium hirsutum species. Sequential 1% H 2 SO 4 pretreatment could further increase biomass digestibility of the steam-exploded stalks, and also cause the highest sugar–ethanol conversion rates probably by releasing less inhibitor to yeast fermentation. By comparison, extremely high concentration alkali (16% NaOH) pretreatment with raw stalks resulted in the highest hexoses yields, but it had the lowest sugar–ethanol conversion rates. Characterization of wall polymer features indicated that biomass saccharification was enhanced with steam explosion by largely reducing cellulose DP and extracting hemicelluloses. It also showed that cellulose crystallinity and arabinose substitution degree of xylans were the major factors on biomass digestibility in cotton stalks. Hence, this study has provided the insights into cell wall modification and biomass process technology in cotton stalks and beyond.
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高影响因子
JOURNAL OF MAGNETIC RESONANCE IMAGING
·
2025
·Vol. 62
·期 4
·1219-1229
·
IF 3.5
Kai Huang,Xiao-Zhu Hao,Cai-Xia Fu,Zhong Yang,Yan Ren,Xue Yang,Shi-Ji Zhang,Mei-Lin Zhu,Zhen-Wei Yao,Da-Xiu Wei,Ye-Feng Yao
Background Glycine (Gly) is a key metabolic intermediate in the proliferation of tumor cells. Monitoring the concentration of Gly in tumor tissues is of great importance for understanding the growth status of tumors. At present, magnetic resonance spectroscopy (MRS) is the only method to non-invasively measure Gly concentration in human tissues. However, in conventional MR spectra the 1 H signal of Gly overlaps with those of other molecules. This makes conventional MRS difficult to accurately measure the Gly concentration in human tissues. Purpose To develop a pulse sequence, Gly-MRS, which can accurately measure Gly concentrations without the influence of the signal overlapping from other molecules in subjects with glioma. Study Type Prospective. Subjects/Phantoms A phantom of the glycine (Gly), myo-inositol (MI) and glutamate (Glu) mixture aqueous solution and 6 phantoms of Gly aqueous solution (pH = 7.2 ± 0.1), 6 subjects with glioma (3 females and 3 males, BMI: 20 ± 4 kg/m 2 , age: 50 ± 10 years). Field Strength/Sequence 3 Tesla/A Gly-targeted magnetic resonance spectroscopy pulse sequence, Gly-MRS, using Point-RESolved Spectroscopy (PRESS) for single voxel signal selection. Assessment By applying the developed pulse sequences to the phantoms and the subjects with glioma, the Gly 1 H signals were successfully selectively probed. Quantification of the signals yields the concentrations of Gly in the regions of the tumor tissues of the subjects with glioma. Statistical Tests Numerical data only. Results The Gly 1 H signals were detected in the tumor regions of 6 subjects with glioma, at a mean concentration of 5.20 mM (standard deviation, ± 3.29 mM). One subject exhibited a clear spatial distribution in the Gly concentrations in the tumor regions. Data Conclusion The Gly-MRS pulse sequence developed in this work might be useful for the accurate in vivo detection of the 1 H signal of Gly in gliomas of human beings. Evidence Level 2. Technical Efficacy Stage 3.
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