ACS Biomaterials Science & Engineering
·
2024
·
IF 5.4
Jiahao Du,Xiaoting Zhang,Wenlong Li,Miao Wang,Xi Zhou,Lei Ren
Blood-contacting catheters play a pivotal role in contemporary medical treatments, particularly in the management of cardiovascular diseases. However, these catheters exhibit inappropriate wettability and lack antimicrobial characteristics, which often lead to catheter-related infections and thrombosis. Therefore, there is an urgent need for blood contact catheters with antimicrobial and anticoagulant properties. In this study, we employed tannic acid (TA) and 3-aminopropyltriethoxysilane (APTES) to create a stable hydrophilic coating under mild conditions. Heparin (Hep) and poly(lysine) (PL) were then modified on the TA-APTES coating surface using the layer-by-layer (LBL) technique to create a superhydrophilic TA/APTES/(LBL)4 coating on silicone rubber (SR) catheters. Leveraging the superhydrophilic nature of this coating, it can be effectively applied to blood-contacting catheters to impart antibacterial, antiprotein adsorption, and anticoagulant properties. Due to Hep’s anticoagulant attributes, the activated partial thromboplastin time and thrombin time tests conducted on SR/TA-APTES/(LBL)4 catheters revealed remarkable extensions of 276 and 103%, respectively, when compared to uncoated commercial SR catheters. Furthermore, the synergistic interaction between PL and TA serves to enhance the resistance of SR/TA-APTES/(LBL)4 catheters against bacterial adherence, reducing it by up to 99.9% compared to uncoated commercial SR catheters. Remarkably, the SR/TA-APTES/(LBL)4 catheter exhibits good biocompatibility with human umbilical vein endothelial cells in culture, positioning it as a promising solution to address the current challenges associated with blood-contact catheters.
研究领域:
生物材料学
·
医学工程
·
表面化学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Liang Zhang,Rouying Cai,Yao Wang,Chenfang Miao,Menghan Zhang,Chen Zheng,Jianzhong Zhang,Zhengjun Huang,Shaohuang Weng
Low molecular weight heparin (LMWH) plays an important role in the prevention and treatment of venous thromboembolism. Sensitive and facile monitoring of LMWH is of great significance for clinical special populations. Herein, using enoxaparin (Enox) as the typical representative of LMWH, a convenient and accurate fluorescence method was developed for Enox detection using carbon dots with antioxidant activity (A-CDs) as probe and polymyxin B (PMB) as medium agent. Compared with the simple Enox or PMB, the formed complex of PMB and Enox (PMB-Enox) based on the electrostatic interaction exhibited a new absorbance peak at 340 nm. Correspondingly, the absorbance of PMB-Enox overlapped with the exciting wavelength of A-CDs at 350 nm and effectively quenched the fluorescence of A-CDs through inner filter effect (IFE). The decreased fluorescence intensities of A-CDs were controlled by the increased Enox with constant PMB. A linear fitting equation was obtained between the quenching efficiency (QE) of A-CDs and variable contents of Enox in the range of 0.8–25.0 μg/mL. And the limit of detection (LOD) was 0.64 μg/mL. The recovery of the injection of Enox in human plasma was acceptable with rational relative standard deviations. Moreover, this fluorescence sensing strategy possesses the prospect of developing reliable pharmaceutical analysis method based on the consideration of drug’s property and pharmacological action and the controllable design of fluorescence probes.
研究领域:
医学检验
·
药物分析
·
生物化学
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产品关联
DOI
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Yueji Li,Yuteng Zhang,Xue Liu,Bin Dong,Pan Xu,Bo Song
Heparin is widely used to treat thrombosis because it is an effective anticoagulant. However, excessive use of heparin can lead to an increased risk of bleeding, which makes the quantitative detection of heparin very important. An amphiphilic perylene bisimide molecule (denoted PBI-9) was developed, which presented a near-infrared emission peak at 730 nm when it was aggregated. PBI-9 has been utilized in the detection of heparin, as the pyridine cation in PBI-9 binds strongly to highly sulfated heparin through electrostatic interactions. Charge neutralization triggers the self-assembly of PBI-9, which leads to an emission peak shift from 540 nm to 690 nm, corresponding to monomeric emission to excimer emission. This shift was applied for the detection of heparin. Importantly, the interaction between PBI-9 and heparin is not affected by protamine, resulting in high selectivity and minimal interference. PBI-9 is a promising tool for detecting heparin in clinical settings after excess heparin has been neutralized by protamine.
研究领域:
生物化学
·
医学诊断
·
药物分析
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Yifei Zhu,Lili Yao,Zongguang Liu,Wenjian Weng,Kui Cheng
A facile strategy is needed for accurate time–space supply of suitable growth factors or drugs. Polypyrrole (PPy) was able to carry almost all kinds of negatively charged biomolecules through anodizing method, which made it an appropriate way for codeposition of multiple molecules. The difference in the conjugation between different molecules and PPy makes it possible for selective release when the redox state of PPy changes. In this work, bovine serum albumin (BSA) and heparin (Hep) were chosen to be the model molecules in view of their differences in the level of electronegativity and molecular weight. Double-layer deposition method was used to improve the biocompatibility of PPy/BSA/Hep film. It was found the content of BSA and Hep in the film can be controlled by regulating deposition current and time. BSA release was facilitated under positive voltage and then promote the proliferation of preosteoblasts, while Hep release was promoted under negative voltage and enhance cell differentiation. Our work provides a dual-molecule model in PPy for selective release and further explores the mechanism of release selectivity, this discovery has potential applications in tissue engineering and regenerative medicine.
研究领域:
生物材料学
·
组织工程学
·
再生医学
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DOI
PubMed
高影响因子
TALANTA
·
2020
·
IF 5.339
Li Jun Gong,Yuan Fang Li,Hong Yan Zou,Cheng Zhi Huang
Heparin (Hep) is a mucopolysaccharide sulfate anticoagulant drug widely used in clinic and thus the quantification of Hep concentration is of great significance. In this work, a simple resonance light scattering (RLS) method for the determination of Hep concentration using plasmonic copper selenide nanoparticles (Cu 2-x Se NPs) was developed. The positively-charged Cu 2-x Se NPs capped by cetyltrimethyl ammonium bromide (CTAB) were aggregated by negatively-charged Hep, consequently inducing a significant enhancement in the RLS signals of plasmonic Cu 2-x Se NPs. Under the optimal reaction conditions, the increased RLS intensity is linearly correlated with the concentrations of Hep in the range of 0.01–0.60 μg mL −1 (R 2 = 0.999), with a low detection limit (LOD) of 4.0 ng mL −1 (3σ). This method offered a simple, sensitive and selective strategy for determining Hep, which can be further successfully applied to quantify Hep in the heparin sodium injection.
研究领域:
分析化学
·
生物医学工程
·
药物分析
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Shao-Bin He,Quan-Quan Zhuang,Liu Yang,Mei-Ying Lin,Ye Kuang,Hua-Ping Peng,Hao-Hua Deng,Xing-Hua Xia,Wei Chen
A visual assay for the detection of heparinase was developed on the basis of a ternary system of Hg2+–heparin–osmium nanoparticles (OsNPs). First, heparin-capped OsNPs (heparin–OsNPs) were synthesized by a facile reduction method using heparin as the protecting/stabilizing agent. The oxidase-like activity of heparin–OsNPs, however, turned out to be low, which somewhat limits their application. We discovered that Hg2+ can significantly/specifically boost the oxidase-like activity of heparin–OsNPs via electrostatic interaction. The oxidase-like activity of heparin–OsNPs toward the oxidation of the substrate, 3,3′,5,5′-tetramethylbenzidine, by dissolved O2 was found to increase by 76-fold in the presence of Hg2+. More significantly, heparin in heparin–OsNPs could be specifically hydrolyzed into small fragments in the presence of heparinase, which resulted in the weakening of the oxidase-like activity of Hg2+/heparin–OsNPs. On the basis of these findings, a linear response of the sensor for heparinase was obtained in the range 20–1000 μg/L with a low detection limit (15 μg/L), which is comparable to those of other reported sensors. Further, the colorimetric sensor was employed for the detection of heparinase in human serum samples with satisfactory results. We speculate that combining such surface modification of the osmium nanozyme with a sensing element could be an interesting direction for promoting nanozyme research in medical diagnosis.
研究领域:
生物化学
·
医学诊断
·
纳米生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Physical Chemistry C
·
2018
·
IF 4.484
Sisheng Hu,Shuibin Shao,Huan Chen,Jiajia Sun,Jing Zhai,Hanyu Zheng,Mimi Wan,Yuhong Liu,Chun Mao,Jing Zhao
In this case, the development of Janus heparin-loaded ammoniated-hollow mesoporous silica (H-A-HMS) nanomotors was reported. First, A-HMS nanoparticles were prepared by combination of hard template (Fe3O4 regarded as core), soft template (cetyltrimethylammonium bromide (CTAB) regarded as mesoporous template agent) and ammoniated process. Then, the heparin was loaded into A-HMS nanoparticles by a simple soaking process, and Janus H-A-HMS nanomotors were fabricated by platinum (Pt) decorating onto the partial surface of H-A-HMS nanoparticles, which the bubble-propelled motion was obtain by the help of Pt catalytic decomposition of hydrogen peroxide (H2O2) into oxygen (gas) and water. Finally, results of a performance test indicated that the H-A-HMS nanomotors we proposed had good blood compatibility, noncytotoxicity, high load and controlled release of heparin, and autonomous motion ability. They will bring great potential for more applications of heparin in the biomedical field.
研究领域:
纳米医学
·
材料科学
·
生物医学工程
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Lipeng Qiu,Xiaotian Shan,Miaomiao Long,Kamel S. Ahmed,Li Zhao,Jing Mao,Huijie Zhang,Changling Sun,Chongzhao You,Guozhong Lv,Jinghua Chen
Heparosan polysaccharide , known as a heparin precursor, can be used in drug delivery systems due to its good biocompatibility and anti-cancer effect. But few studies on the cellular uptake mechanism of heparosan polysaccharide-based nanocarrier have been investigated. Therefore, the intracellular trafficking and the uptake mechanism of heparosan-based micelles by different tumor cells were investigated in this study. Heparosan-cholesterol amphipathic conjugates (KC) were constructed and doxorubicin (DOX) was loaded to prepare DOX/KC micelles. Different cancer cells were selected to find out the influence on DOX/KC uptake. There was an obvious difference in cytotoxicity and cellular uptake of DOX/KC in various cancer cells. Interestingly, DOX/KC micelles exhibited the strongest cytotoxicity against MGC80-3 cells and displayed highly cellular uptake by B16 cells. The results of the uptake mechanism showed that the internalization of DOX/KC micelles into MGC80-3 cells and A549 cells was mainly through clathrin-mediated endocytosis and macropinocytosis , while micropinocytosis , clathrin-mediated endocytosis and clathrin/caveolae -independent multi-pathways all contributed to the uptake of micelles in B16 cells. Furthermore, after being internalized by MGC80-3 cells, DOX/KC could escape from the lysosome and simultaneously be transported into the nucleus and mitochondria resulting in the greatest cytotoxicity. These results indicate that heparosan-based drug delivery systems may have different uptake and subcellular distribution behavior in tumor cells, and they will achieve the maximum efficacy only in specific kind of cancers.
研究领域:
药理学
·
肿瘤学
·
生物材料学
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产品关联
DOI
PubMed
高影响因子
Marine Drugs
·
2021
·Vol. 19
·期 3
·162
·
IF 5.118
Xiang Shi,Ruowei Guan,Lutan Zhou,Zhichuang Zuo,Xuelin Tao,Pin Wang,Yanrong Zhou,Ronghua Yin,Longyan Zhao,Na Gao,Jinhua Zhao
Unique fucosylated glycosaminoglycans (FG) have attracted increasing attention for various bioactivities. However, the precise structures of FGs usually vary in a species-specific manner. In this study, HfFG was isolated fromHolothuria floridanaand purified by anion exchange chromatography with the yield of ~0.9%. HfFG was composed of GlcA, GalNAc and Fuc, its molecular weight was 47.3 kDa, and the -OSO3−/-COO−molar ratio was 3.756. HfFG was depolymerized by a partial deacetylation–deaminative cleavage method to obtain the low-molecular-weight HfFG (dHfFG). Three oligosaccharide fragments (Fr-1, Fr-2, Fr-3) with different molecular weights were isolated from the dHfFG, and their structures were revealed by 1D and 2D NMR spectroscopy. HfFG should be composed of repeating trisaccharide units -{(L-FucS-α1,3-)d-GlcA-β1,3-d-GalNAc4S6S-β1,4-}-, in which sulfated fucose (FucS) includes Fuc2S4S, Fuc3S4Sand Fuc4Sresidues linked to O-3 of GlcA in a ratio of 45:35:20. Furthermore, the heparanase inhibitory activities of native HfFG and oligosaccharide fragments (Fr-1, Fr-2, Fr-3) were evaluated. The native HfFG and its oligosaccharides exhibited heparanase inhibitory activities, and the activities increased with the increase of molecular weight. Additionally, structural characteristics such as sulfation patterns, the terminal structure of oligosaccharides and the presence of fucosyl branches may be important factors affecting heparanase inhibiting activity.Keywords:Holothuria floridana;fucosylated glycosaminoglycans;oligosaccharide;chemical structure;heparanase
研究领域:
海洋生物学
·
生物化学
·
药理学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2012
·Vol. 137
·期 16
·3653-3658
·
IF 4.23
Xiuli Fu,Lingxin Chen,Jinhua Li
A novel colorimetric method was developed for ultrasensitive detection of heparin based on self-assembly of gold nanoparticles (AuNPs) onto the surface of graphene oxide (GO). Polycationic protamine was used as a medium for inducing the self-assembly of citrate-capped AuNPs on GO through electrostatic interaction, resulting in a shift in the surface plasmon resonance (SPR) absorption of AuNPs and exhibiting a blue color. Addition of polyanionic heparin disturbed the self-assemble of AuNPs due to its strong affinity to protamine. With the increase of heparin concentration, the amounts of self-assembly AuNPs decreased and the color changed from blue to red in solution. Therefore, a “blue-to-red” colorimetric sensing strategy based on self-assembly of AuNPs could be established for heparin detection. Compared with the commonly reported aggregation-based methods (“red-to-blue”), the color change from blue to red was more eye-sensitive, especially in low concentration of target. Moreover, stronger interaction between protamine and heparin led to distinguish heparin from its analogues as well as various potentially coexistent physiological species. The strategy was simply achieved by the self-assembly nature of AuNPs and the application of two types of polyionic media, showing it to be label-free, simple, rapid and visual. This method could selectively detect heparin with a detection limit of 3.0 ng mL−1 in standard aqueous solution and good linearity was obtained over the range 0.06–0.36 μg mL−1 (R = 0.9936). It was successfully applied to determination of heparin in fetal bovine serum samples as low as 1.7 ng mL−1 with a linear range of 0–0.8 μg mL−1.
研究领域:
分析化学
·
生物医学工程
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2012
·
IF 3.456
Ping Liang,Dong Zhao,Chao-Qun Wang,Jing-Yi Zong,Ren-Xi Zhuo,Si-Xue Cheng
A facile method to prepare inorganic/organic hybrid heparin/CaCO 3 /CaP nanoparticles for drug delivery was developed. The heparin/CaCO 3 /CaP nanoparticles were prepared by the co-precipitation of Ca 2+ ions with carbonate and phosphate ions in the presence of heparin. The effects of ion concentrations on the particle size and properties of the nanoparticles were investigated. The dynamic light scattering (DLS) particle size analysis and scanning electron microscopy (SEM) observation showed that the mean size of the hybrid nanoparticles could be controlled at less than 50 nm in the dried state through adjusting the concentrations of the inorganic ions. Fourier transform infrared (FTIR) spectroscopy indicated that the crystallization of CaCO 3 could be suppressed with the presence of phosphate ions in the co-precipitation system. X-ray photoelectron spectroscopy (XPS) showed that the heparin content in the surface layer of heparin/CaCO 3 /CaP hybrid nanoparticles decreased with the increasing ion concentrations during the nanoparticle preparation. The drug loading and release properties of the hybrid nanoparticles with different sizes and different compositions were studied. The in vitro cellular cytotoxicity indicated that blank hybrid nanoparticles had good biocompatibility, whereas doxorubicin hydrochloride (DOX) loaded nanoparticles exhibited a strong cell inhibition effect, indicating that the heparin/CaCO 3 /CaP hybrid nanoparticles could be promising carriers for drug delivery.
研究领域:
材料科学
·
药学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2017
·
IF 3.387
Hui Wang,Xiao Shi,Ailin Gao,Haibo Lin,Yongliang Chen,Yumin Ye,Jidong He,Fu Liu,Gang Deng
In the present work, we report one-step immobilization of nano-heparin coating on PLA membranes via initiated chemical vapor deposition (iCVD) for enhanced hemocompatibility. The nano-coating introduced onto the membrane surface via the crosslinking of P(MAA-EGDA) was confirmed by the FTIR, SEM and weight measurement respectively. The negative carboxyl groups could form the hydration interaction with the protein and platelets and electrostatic interaction with amide groups of thrombin by the mediation of antithrombin, which is similar but different with heparin. The P(MAA-EGDA) coated membranes showed suppressed platelet adhesion and prolonged clotting time (APTTs increased to 59 s, PTs increased to 20.4 s, TTs increased to 17.5 s, and the FIBs declined by 30 mg/dL). Moreover, the complement activation tests demonstrated the formation of C3a and C5a was inhibited. All results demonstrated that the nano-coating of P(MAA-EGDA) via iCVD significantly enhanced the hemocompatibility of PLA membranes, which is also applicable for various membranes.
研究领域:
生物材料
·
医学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）