JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2022
·
IF 4.598
Tongtong Zhai,Yuan Xue,Yunhui Li,Jianwei Zhu,Jing Li,Erkang Wang
For hot electron-induced electrochemiluminescence (HECL), developing facile and controllable insulating layer on the electrode surface is key to the effective electron tunneling. Herein, a polystyrene modified glassy carbon electrode based the spin-coating was proposed for HECL . The constructed interface was characterized by atomic force microscopy (AFM). And HECL performance was investigated in details using the system of Ru(bpy) 3 2+ and S 2 O 8 2− . The as-prepared film shows the stable and reproducible HECL performance and the HECL mechanism was discussed using ECL spectra and hydrated electron quenchers. Finally, on the basis of the inhibition of SO 4 − , the present polystyrene film was applied to the detection of rutin in the range of 0.1–1.3 μM with a detection limit of 90 nM (S/N = 3).
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 43
·20610-20622
·
IF 3.925
Rongqian Meng,Yanfeng Xue,Lijun Guo,Hong Yang,Yongbo Song,Qiaoling Li,Yulan Niu
A novel electrochemical sensor was constructed based on an overoxidized polypyrrole/overoxidized poly(3,4-ethylenedioxythiophene)/graphene quantum dots-chitosan composite film modified glass carbon electrode (oPPy/oPEDOT/GQDs-CS/GCE), for the accurate determination of luteolin in peanut shells. The oPPy/oPEDOT/GQDs-CS/GCE was prepared using layer-by-layer assembly methods. After incubation in NaOH–EtOH solution, the current of luteolin on the composite electrode increased from 0 to ∼30 μA, the Rct value decreased by ∼80 fold, and the specific surface area (A) increased by ∼7 fold. Based on a three-level four-factor Box–Behnken design (BBD), the optimal preparation conditions for the composite electrode were as follows: a mass ratio of CS to GQDs of 5, a polymerization cycle number for EDOT of 4, a polymerization cycle number for Py of 4, with the peak current increased to 43.21 μA (RSD = 3.51%, n = 3). A morphology with multi-channels and grooves was observed on the surface of the incubated composite film via SEM analysis. The additional –CN– and –SO/–SO2 groups formed were observed by XPS and FTIR analysis. The electrochemical sensor constructed exhibited good anti-interference ability, repeatability, and stability. The linear detection range recorded by the current responses of the composite electrode to luteolin was 0.01 to 10.0 μM (R2 = 0.991) and the limit of detection was 7.5 nM (S/N = 3). The observed content of luteolin in peanut shells was 0.769%, which was close to the results obtained from High Performance Liquid Chromatography (HPLC) analysis (0.808%). This agreement indicated that the constructed electrochemical sensor based on oPPy/oPEDOT/GQDS-CS/GCE was capable of accurate detection of luteolin in peanut shells.
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Frontiers in Nutrition
·
2022
·
IF 6.59
Hui Jiang,Fei Wu,Xi Jiang,Yun-Feng Pu,Li-Rong Shen,Cui-Yun Wu,Hong-Jin Bai
Pear is one of the most popular fruits in the world. With the fruit ripening, a series of physiological changes have taken place in fragrant pear, but up to now, the research on the metabolism and biological activity of phenolic compounds in different growth stages of fragrant pear is still lacking. In this study, four kinds of Xinjiang pears were selected as research objects, and the changes of phenolic content, antioxidant capacity, cell protection and whitening activity during fruit development were analyzed. The results showed that the phenolic content and antioxidant capacity of four pear varieties presented a decreasing trend throughout the developmental stages. The phenolic content and antioxidant activity of the four pears in the young fruit stage were the highest, and the active ingredients of the Nanguo pear were higher than the other three pear fruits. Pear extract could protect cells by eliminating excessive ROS in cells, especially in young fruit stage. The western blot results showed that the extract of fragrant pear in the young fruit stage could inhibit the expression of TYR, TYR1 and MITF in B16 cells, and it was speculated that the extract of fragrant pear in the young fruit stage might have good whitening activity. Therefore, the findings suggest that young pear display a good antioxidant potential and could have a good application prospect in food preservation and health product industry.
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高影响因子
Italian Journal of Animal Science
·
2022
·
IF 2.552
Shun Chen,Huijuan Liu,Jiaqi Zhang,Binbin Zhou,Su Zhuang,Xiaofang He,Tian Wang,Chao Wang
This study investigated the effects of different doses of rutin on the growth performance, immunity, intestinal barrier, and antioxidant capacity of broilers. A total of two hundred and fifty-six 1-day-old male broilers were divided into 4 groups and fed basal diets supplemented with 0 (control group), 250, 500 and 1,000 mg rutin/kg, respectively, for 42 days. In the starter period (days 1–21) and whole trial period (days 1–42), broilers fed diets containing 500 mg rutin/kg had significantly (p < 0.05) higher average daily feed intake, body weight and average daily gain and better feed conversion ratio. Dietary 500 mg rutin/kg increased the villus height, villus height to crypt depth ratio and villus area, while reducing the B cell lymphoma 2 associated X mRNA expression (p < 0.05). Dietary 500 mg rutin/kg increased the level of IgA in serum and decreased serum tumour necrosis factor-α (TNF-α) content, and the nuclear factor kappa-B, interleukin-2 and TNF-α mRNA expression in jejunal mucosa (p < 0.05). Simultaneously, 500 and 1,000 mg rutin/kg significantly decreased serum diamine oxidase activity, D-lactic acid and lipopolysaccharide concentration, increased activities of total superoxide dismutase and total antioxidant capacity in jejunal mucosa (p < 0.05), and upregulated mRNA expressions of genes related to intestinal barrier and antioxidant capacity in jejunal mucosa (p < 0.05). These findings indicate that dietary rutin, especially at 500 mg/kg, improves broilers’ growth performance, intestinal barrier function, immunity, and antioxidant capability, which may be related to the nuclear factor erythroid-2-related factor 2 (Nrf2) pathway.HighlightsDietary rutin improved growth performance, jejunal morphology, and intestinal barrier function.Dietary rutin enhanced the immunity via inhibiting NF-κB, and improved antioxidant capacity via activating Nrf2/HO-1 pathway in broilers.The optimal dose of rutin was 500 mg/kg in broiler diet.
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RSC Advances
·
2022
·Vol. 12
·期 38
·25025-25034
·
IF 4.036
Ruijing Wei,Lu Hu,Lihua Wang,Peng Yan,Tao Lin,Ning Wang,Huaiqing Sun,Bisheng Zheng,Chaowan Guo
In this study, deep eutectic solvents coupled with a pulsed electric field (PEF-DES) were first applied to the extraction of traditional Chinese medicine plants. This study uses the PEF-DES extraction technique to extract TG-KF (Kapok flavonoid solution extracted with DES-TG). PEF-DES is a simple, effective and environmentally-friendly technology and can be used in industrial-scale production. For the optimal extraction conditions of TG-KF, DES-TG was used as a solvent, the DES-TG concentration was 50%, the solid–liquid ratio was 1 : 30, the electric field intensity was 0.55 kV cm−1, the number of pulses was 100, and the yield of flavonoids was 14.36 ± 0.35%. TG-KF has very good stability and there is no precipitation or discoloration within 6 months. The results of chicken embryo experiments and human patch tests show that 10% TG-KF aqueous solution has no irritation. DPPH experiments show that TG-KF has excellent efficacy as an antioxidant. Overall, TG-KF is expected to become a potential antioxidant raw material.
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CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Shucheng Liu,Ying Sun,Danzhao Guo,Ruiheng Lu,Yuying Mao,Hongxiang Ou
For porous molecular imprinted polymers (MIPs), introduction of special functional groups and integration porous structure support will endow the MIPs with tunable surface properties and specific affinity performance. Herein, by combination of RAFT molecular imprinting, Pickering emulsion, and boronate affinity methods, a new kind of porous imprinted microspheres (BA-HPMIPs) using the low pKa value of APBA (1-allylpyridinium-3-boronic acid, pKa = 7.4) as functional monomer was prepared for specific molecular recognition and separation of NRG. Meanwhile, the novel RAFT functioned surfactants with dual amide multiple H-bonds were designed as stabilized particles was used to promote sustainability, which further confer them multifunctionality and/or by polymerizing the Pickering emulsion itself. Equilibrium adsorption was reached rapidly within 360 min, and the maximum equilibrium binding capacities of BA-HPMIPs were found to be 118.32 µmol g −1 for NRG. Furthermore, they had a stronger selectivity towards NRG than other competitive target (i.e rutin, catechol, ARS, o -nitrophenol). Moreover, BA-HPMIPs have shown outstanding reusability of 8.63 % loss in enrichment performance after five times repeatedly. As a proof of this concept, a commercially avaliable NRG with purity could be effectively extracted and concentrated to 96.75 % purity. These results demonstrated that, a new kind of porous imprinted microsphere with abundant porous structure and boronic acid recognizing sites has great potential for separation and purification of industrial samples under neutral conditions.
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BIOMEDICAL CHROMATOGRAPHY
·
2022
·Vol. 37
·期 2
·e5536
·
IF 1.911
Qi Dong,Na Hu,Huilan Yue,Honglun Wang,Yue Wei
α -glucosidase inhibitors (AGIs) are widely used for the treatment of type 2 diabetes, but their side effects have made it to develop novel and alternative AGIs immediately. In this study, the extract of Hypericum perforatum L. (HPE) has been confirmed to have α -glucosidase inhibitory activity in vitro and in vivo. Seven active compounds, rutin, hyperoside, isoquercitrin, avicularin, quercitrin, quercetin, and biapigenin, were screened based on a bio-affinity chromatography column with α -glucosidase enzyme-conjugated solid phase and UPLC/MS, which exhibited excellent α -glycosidase inhibitory effects by the determined IC 50 values. The mechanism of α -glycosidase inhibitory activity of biapigenin was studied for the first time. The results showed that biapigenin was a high-potential, reversible, and mixed enzyme inhibitor. Analysis by molecular docking further revealed that hydrophobic interactions were generated by interactions between biapigenin and amino acid residues LYS156, PHE303, PHE314, and LEU313. In addition, hydrogen bonding occurred between biapigenin and α -glucosidase amino acid residues ASP307, SER241, and LYS156. This research identified that biapigenin could be a novel AGI and further applied to the development of potential anti-diabetic drugs. Furthermore, our studies established a rapid in vitro screening method for AGIs from plants.
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AMB Express
·
2022
·Vol. 12
·期 1
·1-14
·
IF 4.126
Yu Bo,Luo Shiyu,Ding Yuhan,Gong Zijie,Nie Ting
αL-rhamnosidase (EC 3.2.1.40) has been widely used in food processing and pharmaceutical preparation. The recombinant α-L-rhamnosidase N12-Rha from Aspergillus niger JMU-TS528 had significantly higher catalytic activity on α-1,6 glycosidic bond than α-1,2 glycosidic bond, and had no activity on α-1,3 glycosidic bond. The activities of hydrolyzed hesperidin and naringin were 7240 U/mL and 945 U/mL, respectively, which are 10.63 times that of native α-L-rhamnosidase. The activity could maintain more than 80% at pH 3–6 and 40–60℃. Quantum chemistry calculations showed that charge difference of the C-O atoms of the α-1,2, α-1,3 and α-1,6 bonds indicated that α-1,6 bond is most easily broken and α-1,3 bond is the most stable. Molecular dynamics simulations revealed that the key residue Trp359 that may affect substrate specificity and the main catalytic sites of N12-Rha are located in the (α/α) 6 -barrel domain.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Rong Tian,Lan Zhou,Naihao Lu
The interactions between quercetin and bovine (or human) hemoglobin (Hb) were systematically investigated by fluorescence, UV–vis absorption spectroscopy, and molecular docking to demonstrate the structural mechanism by which quercetin affected the Hb redox state and stability. Quercetin could interact with the central cavity of the Hb molecule with one binding site to generate an Hb–quercetin complex, and the hydrophobic interaction played an important role in the formation of the complex. The binding constant for the Hb–quercetin complex at 298 K was observed to be 1.25 × 104 M–1. In addition, quercetin effectively inhibited Hb-induced lipid oxidation in liposomes or washed muscles, which was ascribed to the conversion to oxy-Hb and decreased hemin dissociation from met-Hb. Consistent with its lower abilities to bind Hb and scavenge free radicals, rutin (i.e., quercetin-3-rhamnosylglucsoside) did not significantly influence the redox state of Hb nor reduce hemin release from Hb, and subsequently, it less effectively inhibited Hb-induced lipid oxidation than quercetin. Altogether, the results herein provide novel insights into the antioxidant mechanism for quercetin and are beneficial to the application of natural quercetin in Hb-containing foods.
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高影响因子
MOLECULES
·
2022
·Vol. 27
·期 22
·8021
·
IF 4.927
Tao Zhang,Qingxue Ji,Jiayi Song,Haiming Li,Xing Wang,Haiqiang Shi,Meihong Niu,Tingting Chu,Fengshan Zhang,Yanzhu Guo
The poor water solubility, large particle size, and low accessibility of cellulose, the most abundant bioresource, have restricted its generalization to carbon dots (CDs). Herein, nitrogen and sulfur co-doped fluorescent carbon dots (N, S-CDs) were hydrothermally synthesized using cellulose nanocrystals (CNC) as a carbon precursor, exhibiting a small particle size and excellent aqueous dispersion. Thiourea was selected as a nitrogen and sulfur dopant to introduce abundant fluorescent functional groups into N, S-CDs. The resulting N, S-CDs exhibited nanoscale size (6.2 nm), abundant functional groups, bright blue fluorescence, high quantum yield (QY = 27.4%), and high overall yield (16.2%). The excellent optical properties of N, S-CDs endowed it to potentially display a highly sensitive fluorescence “turn off” response to rutin. The fluorescence response for rutin allowed a wide linear range of 0–40 mg·L−1, with a limit of detection (LOD) of 0.02 μM, which revealed the potential of N, S-CDs as a rapid and simple sensing platform for rutin detection. In addition, the sustainable and large-scale production of the N, S-CDs in this study paves the way for the successful high-value utilization of cellulose.Keywords:cellulose nanocrystals;carbon dots;hydrothermal method;fluorescent sensing
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NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 47
·22621-22632
·
IF 3.925
Yifei Zhou,Qiuxiao Wang,Tao Wang,Haixiang Gao
Recently, 2,4-dichlorophenoxyacetic acid, 4-chlorophenoxyacetic acid, and 2-methyl-4-chlorophenoxyacetic acid have been extensively used as herbicides in field crops and they can also act as a plant growth regulator at low concentrations. However, these compounds possess high vapor pressure and high volatility. In this regard, the introduction of ionic liquids (ILs) has emerged as an effective approach to overcome these shortcomings. The present study aimed to prepare a series of ILs with bactericidal activity cations and phenoxyacetic acid series compounds as anions. The synthesized compounds were tested for their physicochemical properties, antibacterial activity and citrus preservation through experiments. ILs showed lower volatility, better soil adsorption capacity, and dual functions of plant growth regulation and antibacterial activity. The tested ILs effectively delayed the aging rate of citrus, reduced the decay rate, inhibited citrus storage diseases, and improved the storage quality of citrus. Overall, the study results provide a theoretical basis for the development of sustainable multifunctional ILs by introducing anions and cations with targeted effects. These newly synthesized ILs allowed us to achieve benefits far exceeding the simple additive rule, improving the physical and chemical properties and reducing environmental pollution by decreasing the amount of chemicals.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Alkassoumi Hassane Hamadou,Jiyao Zhang,Chen Chao,Bin Xu
This work evaluated the effect of pectin (P) and chitosan (CH) dual coating on rutin (R) loaded nanoliposomes (NLs). Besides R loaded NLs (RNLs), CH coated RNLs (CH-RNLs) and P coated CH-RNLs (P–CH-RNLs) were prepared. The results showed a size increase of NLs due to the polymer coatings, confirming a successful deposition of CH and P onto the surfaces of NLs. A surface charge reversal was observed after coating RNLs and CH-RNLs with CH and P, respectively. The coating resulted in a significant decrease in encapsulation efficiency (EE) from 88.19% in RNLs to 78.89% in CH-RNLs and 73.44% in P–CH-RNLs. Due to protection provided by the P–CH barrier, P–CH-RNLs displayed better thermal, pH, ionic and oxidative stability. NLs were more stable at 4 °C as compared to 25 °C over 8 weeks, and P–CH-RNLs exhibited the best performance overall. This study revealed P–CH coating as a potential route to produce stable NLs for the delivery of hydrophobic rutin.
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危险品化学品经营许可证（带存储）