Nanomaterials
·
2021
·Vol. 11
·期 11
·3060
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IF 5.076
Jinxing Zhang,Qi Ouyang,Qilin Gui,Xiaonong Chen
Scalable preparation of graphene with high adsorption capacity is an important prerequisite for fully realizing its commercial application. Herein, we propose an environmentally friendly route for exfoliation of graphene, which is established based on the Diels–Alder reaction. In our route,N-(4-hydroxyl phenyl) maleimide enters between the flakes as an intercalating agent and participates in the Diels–Alder reaction as a dienophile to increase the interlayer spacing of graphite. Then, graphite is exfoliated into graphene with the aid of ultrasound. The exfoliated product is hydroxyl phenyl functionalized graphene with a thickness of 0.5–1.5 nm and an average lateral size of about 500–800 nm. The exfoliation process does not involve any acid or catalyst and would be a safe and environmentally friendly approach. In addition, the exfoliated graphite shows high resveratrol adsorption capacity, which is ten times that of macroporous resins reported in the literature. Thus, the method proposed herein yields exfoliated graphite with high resveratrol adsorption capacity and is of great significance for the mass production of graphene for practical applications.Keywords:graphene;Diels–Alder reaction;exfoliation;resveratrol;adsorption
研究领域:
材料科学
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环境科学
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DOI
PubMed
高影响因子
Antioxidants
·
2022
·Vol. 11
·期 4
·647
·
IF 7.675
Xin Yin,Hao Cheng,Wusigale,Huanhuan Dong,Weining Huang,Li Liang
The interaction of protein carrier and polyphenol is variable due to their environmental sensitivity. In this study, the interaction between resveratrol and whey protein isolate (WPI), sodium caseinate (SC) and soy protein isolate (SPI) during storage were systematically investigated from the aspects of polyphenol loading, antioxidant activity and oxidability. It was revealed that resveratrol loaded more in the SPI core and existed both in the core of SC micelles and on the particle surface, while WPI and resveratrol mainly formed in complexes. The loading capacity of the three proteins ranked in order SC > SPI > WPI. ABTS assay showed that the antioxidant activity of the protein carriers in the initial state was SC > SPI > WPI. The results of sulfhydryl, carbonyl and amino acid analysis showed that protein oxidability was SPI > SC > WPI. WPI, with the least oxidation, improved the storage stability of resveratrol, and the impact of SC on resveratrol stability changed from a protective to a pro-degradation effect. Co-oxidation occurred between SPI and resveratrol during storage, which refers to covalent interactions. The data gathered here suggested that the transition between the antioxidant and pro-oxidative properties of the carrier is the primary factor to investigate its protective effect on the delivered polyphenol.Keywords:protein;resveratrol;loading;antioxidant activity;oxidability;stability
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
IRANIAN POLYMER JOURNAL
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2022
·Vol. 31
·期 10
·1225-1235
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IF 2.485
Mehmood Sahid,Uddin Md Alim,Yu Haojie,Wang Li,Amin Bilal Ul,Haq Fazal,Fahad Shah,Haroon Muhammad
Polymeric materials are used as ideal carriers for drug delivery applications due to their excellent physicochemical properties, however, the controlled preparation and drug release behavior of cross-linked polymeric microspheres are still open to discussion. Hexacyclotriphosphazene (HCCP), as one of the prominent members of polyphosphazene, can be easily treated with many monomers to produce micro/nanospheres. In this research work, cross-linked poly(cyclotriphosphazene- co -resveratrol) (PCTPRS) microspheres were prepared by the precipitation reaction of HCCP and resveratrol (RSV). The apparent peak at 961 cm −1 in FTIR spectra of the microspheres revealed that the polymerization between RSV and HCCP was successful. The TGA results showed that the increase in thermal properties was owing to the highly cross-linked structure of the microspheres. The XRD patterns showed the amorphous nature of the microspheres compared to the monomers. The obtained SEM micrographs dictated that the HCCP:RSV mole ratio had a significant impact on the size of the microspheres. The PCTPRS microspheres were used as carriers for the drug loading and release study of the anti-cancer model drug camptothecin (CPT). The drug loading efficiency of the PCTPRS microspheres was found to be 89.9 mg/g. Whereas the release study revealed that 53.0% of CPT was released at pH 4.0 and 37.5% at pH 7.4 from the drug-loaded microspheres after 11 days. Graphical abstract
研究领域:
材料科学
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药学
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生物医学工程
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DOI
高影响因子
FOOD CHEMISTRY
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2023
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IF 8.8
Weigang Zhong,Qi Wang,Xue Shen
Rational selection of the complex state and polysaccharide type may enhance the performance of electrostatic complex stabilized high internal phase emulsions (HIPEs). Herein, quinoa proteins were extracted to form electrostatic complexes separately with three gelling-type polysaccharides to fabricate HIPEs. Results showed that the complexes in soluble state (pH 8.4–5.6) exhibited moderate size, high negative potential and enhanced protein hydrophobicity, and could achieve HIPEs with 84% oil phase upon acidification to pH 6 at low concentrations. Its excellent interfacial structure enhanced stability during heating, freeze-thawing and long-term storage, and exhibited promising 3D printing potential. Furthermore, the complexes formed by sulfated polysaccharide carrageenan had higher amphiphilicity than those formed by carboxylated polysaccharide pectin or sodium alginate, and their stabilized HIPE had preferable droplet size, stability and 3D printing resolution than its counterparts. This study may provide new insights into the performance enhancement of protein/polysaccharide electrostatic complex stabilized HIPEs.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
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2023
·Vol. 17
·期 6
·5594-5605
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IF 3.4
Yu Yuyuan,Shi Yongqing
The interaction between quinoa glycopeptides (QGs) and three polyphenols [(−)-epigallocatechin-3-gallate (EGCG), gallic acid (GA) and tannic acid (TA)] was investigated by particle size, potential, fluorescence spectrum, and Fourier infrared spectrum, and the changes of antioxidant activity after compound were investigated by in vitro antioxidant experiments. The results showed that the average particle size of quinoa glycopeptide decreased and its distribution became more uniform after it was combined with three polyphenols. The intrinsic fluorescence was quenched and decreased with the increase of polyphenol content, and the maximum emission wavelength of the intrinsic fluorescence spectrum was redshifted. When combined with EGCG, GA and TA, the surface hydrophobicity of quinoa glycopeptides decreased significantly. The quenching type of EGCG, GA, and TA on quinoa glycopeptides was static quenching. The addition of polyphenols changed the hydrophobic amino acid groups in quinoa glycopeptides, and the hydrophobic interaction was the main force. Additionally, the secondary structure of QG was slightly changed after the binding of polyphenols to QG. Moreover, DPPH and ABTS radical scavenging abilities and ferric reducing antioxidant power analysis showed that EGCG, GA, and TA enhanced significantly the antioxidant capacity of QG. Therefore, this study can guide the preparation of quinoa functional food.
研究领域:
食品科学
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生物化学
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DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Qiaozhi Zhang,Shiyu Fan,Hujun Xie,Yan Zhang,Linglin Fu
This study aimed to investigate the interaction modes between maize starch (MS) and quinoa polyphenols (QPs) under hydrothermal treatments and its influence on the multi-scale structure and digestive behaviors of MS through mechanistic analyses. Thermodynamic and microscopic results revealed that during starch gelatinization , the starch chains interacted with QP polyphenols to form new aggregates with retarded gelatinization. The X-ray diffraction and 13 C nuclear magnetic resonance spectroscopy further confirmed the presence of both V-type inclusion complexes and non-inclusion complexes in QP-complexed MS. Through multiple spectroscopic approaches, it was demonstrated that complexation with QPs promoted the formation of local helical and short-range ordered structures and crystallization in MS and led to resistance of the starch chains to digestive enzymes. As a consequence, the MS–QP complexes showed substantially reduced digestion rates with increases in the contents of resistant starch and slowly digestible starch, as well as lower values of the predicted glycemic index . Compared to white quinoa polyphenols, polyphenols from red and black quinoa displayed a greater efficiency in modulating the starch structure and digestive properties. This study provides a valuable basis for the application of pigmented quinoa polyphenols as functional ingredients in starchy foods with desired glycemic response.
研究领域:
食品科学
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营养学
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植物化学
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DOI
高影响因子
Microbial Biotechnology
·
2020
·Vol. 13
·期 4
·1118-1131
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IF 5.328
Tao Ma,W. Wu,Y. Tu,N. Zhang,Q. Diao
Summary This study aimed to investigate the effect of resveratrol on methane production, rumen fermentation and microbial composition under high-concentrate (HC) and high-forage (HF) diets using the in vitro fermentation system. A total of 25 mg of resveratrol was supplemented into 300 mg of either HC or HF diet. Methane production, total volatile fatty acid (VFA) concentration, molar proportion of VFA, metabolites of resveratrol and prokaryotic community composition were measured after 12 and 24 h of in vitro fermentation. Resveratrol reduced methane production (ml per mg of dry matter degraded) by 41% and 60% under both HC and HF diets ( P < 0.001), respectively, and this result could be associated with the lower abundance of Methanobrevibacter ( P < 0.001) in response to resveratrol. The molar proportion of propionate was significantly higher in the resveratrol group only under the HC diet ( P = 0.045). The relative abundance of 10 bacterial genera was affected by the three-way interaction of treatment, diet and time ( P < 0.05). Resveratrol was partly converted to dihydroresveratrol after 24 h of fermentation, and its degradation could be associated with microbes belonging to the order Coriobacteriales . Our results suggest that multiple factors (e.g. diet and time) should be considered in animal experiments to test the effect of polyphenol or other plant extracts on rumen fermentation, methane emission and microbial composition.
研究领域:
动物科学
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微生物学
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营养学
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DOI
PubMed
高影响因子
INTERNATIONAL IMMUNOPHARMACOLOGY
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2025
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IF 4.7
Yi-fan Li,Yue-fan Zhang,Can Huang,Jie-mei Jiang
Objective The inflammatory response is integral to all stages of ischemic stroke. Unlike conventional reperfusion therapies, anti-inflammatory strategies offer a broader therapeutic window for treating ischemic stroke due to their capacity to attenuate inflammation. Astrocytes, once activated in ischemic conditions, significantly contribute to the production of inflammatory cytokines and exacerbate brain damage. While the neuroprotective effects of baicalin in post-stroke patients have been recognized, its role in modulating astrocyte activity and reducing inflammation remains under debate. This study aims to evaluate the impact of baicalin on astrocyte activation following ischemic stroke. Methods A model of ischemia/reperfusion (I/R) injury was induced in wild-type mice through transient middle cerebral artery occlusion (tMCAO). Mice were randomized into groups receiving either baicalin or saline. The expression levels of inflammatory markers—interleukin (IL)-6, IL-1β, monocyte chemoattractant protein-1 (MCP-1), and tumor necrosis factor-alpha (TNF-α)—were quantified using enzyme-linked immunosorbent assay (ELISA). Additionally, western blot analysis was employed to assess glial fibrillary acidic protein (GFAP) expression. Results Baicalin administration significantly mitigated neurological deficits in mice post-tMCAO. It reduced the activation of astrocytes and the production of pro-inflammatory cytokines such as IL-6, IL-1β, TNF-α, and MCP-1, observed both in vivo and in vitro. In vitro studies also indicated a suppression of NF-κB activation. Conclusion Baicalin effectively prevents ischemic brain damage by curtailing neuroinflammation and astrocyte activation. These findings advance the understanding of baicalin’s mechanistic role in mitigating brain ischemia and support further investigation into its therapeutic potential.
研究领域:
神经科学
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药理学
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DOI
PubMed
高影响因子
RSC Advances
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2024
·Vol. 14
·期 43
·31768-31776
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IF 3.9
Jiawei Zhao,Li Lu,Qiwei Chai,Wei Jin,Min Zhu,Shengqi Qi,Jiali Shentu,Yuyang Long,Dongsheng Shen
The unique capacity of certain plant endophytes to degrade organic pollutants has garnered considerable interest in recent years. However, it remains uncertain whether endophytes can maintain high degradation activity after in vitro culture and whether they can be used directly in the remediation of contaminated soils. This study reveals that resveratrol, a plant secondary metabolite, selectively boosts the degradation of polycyclic aromatic hydrocarbons (PAHs) by endophytic Methylobacterium extorquens C1 (C1) in vitro, while exerting negligible effects on the activity of indigenous soil bacteria. For the first time, a combined application of C1 and resveratrol was employed in the remediation of polycyclic aromatic hydrocarbon (PAH)-contaminated soil. The findings indicate that the sole use of resveratrol failed to promote the removal of PAHs by indigenous soil microorganisms, whereas sole application of C1 boosted Methylobacterium-related PAH-degrading bacterial abundance, enhancing PAH removal, yet concurrently reduced overall soil microbial diversity. The combination of resveratrol and C1 not only stimulated the PAH removal but also mitigated the impact of C1 on the soil microbial community structure when C1 was applied individually. Specifically, the optimal removal efficacy was achieved with a treatment combination of 5 mg kg−1 resveratrol and 1.2 × 107 CFU kg−1 of C1, leading to a 130% and 231% increase in the removal of phenanthrene and acenaphthene, respectively, over a 15 days period. This study proposes a novel approach for the bioremediation of organic-contaminated soil by using the specific biological response of plant endophytic bacteria to secondary metabolites.
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
BIOSCIENCE REPORTS
·
2024
·Vol. 44
·期 6
·
IF 3.8
Hao Xiao Hua,Liu Ke Xin,Zhang Meng Yuan
Increasing cadmium (Cd) pollution has negative effects on quinoa growth and production. Gamma-aminobutyric acid (GABA) confers plants with stress resistance to heavy metals; however, the mechanism remains unclear. We explored the effects of exogenous GABA on the physiological characteristics, antioxidant capacity, and Cd accumulation of quinoa seedlings under Cd stress using hydroponic experiments. Partial least-squares regression was used to identify key physical and chemical indices of seedlings affecting Cd accumulation. Compared with those of the CK group, exposure to 10 and 25 µmol·L−1 Cd significantly reduced the photosynthetic pigment contents, photosynthesis, and biomass accumulation of quinoa seedlings; resulted in shorter and thicker roots; decreased the length of the lateral roots; decreased the activities of superoxide dismutase (SOD) and peroxide (POD); and increased H2O2 and malondialdehyde (MDA) contents. Exogenous GABA reduced the Cd content in the stem/leaves and roots of quinoa seedlings under Cd stress by 13.22–21.63% and 7.92–28.32%, decreased Cd accumulation by 5.37–6.71% and 1.91–4.09%, decreased the H2O2 content by 38.21–47.46% and 45.81–55.73%, and decreased the MDA content by 37.65–48.12% and 29.87–32.51%, respectively. GABA addition increased the SOD and POD activities in the roots by 2.78–5.61% and 13.81–18.33%, respectively, under Cd stress. Thus, exogenous GABA can reduce the content and accumulation of Cd in quinoa seedlings by improving the photosynthetic characteristics and antioxidant enzyme activity and reducing the degree of lipid peroxidation in the cell membrane to alleviate the toxic effect of Cd stress on seedling growth.
研究领域:
植物生理学
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环境科学
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农业科学
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Longwei Chen,Chi Zhang,Xi Yu,Lixiao Fu,Xiaozhi Tang,Xiao Feng
Native quinoa protein has weak processability. Fibrillation can vary the structure and foaming properties of proteins. Quinoa 2S albumin also has the potential to be an interface active substance. In this study, quinoa protein amyloid-like fibrils (QPF) and 2S albumin were blended to stabilize foams for the first time, and their interfacial behavior, foaming properties and the microstructure of foams were analyzed. The absolute value of zeta potential and surface tension of quinoa protein isolates (QPI) decreased after fibrillation, which improved the foam capacity and stability significantly. When QPF were further blended with 2S albumin at a ratio of 1:1, foam capacity and foam stability reached the highest values of 183.33% and 86.38%, respectively. The adsorption of QPF at the air-water interface along with 2S albumin’s filling in the interfacial voids may strengthen the protein interfacial network and their foaming properties. Meanwhile, our study revealed that both the viscosity of the liquid phase as well as the interfacial properties of the protein dispersion influenced the foam capacity and foam stability of QPF/2S albumin. Furthermore, foams generated from QPF/2S albumin were applied to prepare coffee latte art and chiffon cakes. The cakes using foams generated from QPF/2S albumin to fully replace egg white exhibited a fluffy, porous, and stable structure. To conclude, the fibrillated quinoa protein and 2S albumin complex showed the potential to be an innovative plant-based ingredient to generate foams and applied in food processing industry, which will contribute to the food sustainability.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
Materials
·
2018
·Vol. 11
·期 8
·1407
·
IF 2.467
Siji Chen,Shanshan Tang,Yang Sun,Gang Wang,Huan Chen,Xiaoxiao Yu,Yingjie Su,Guang Chen
A porous carbon material was prepared from quinoa husk (QH) by carbonization and chemical activation with KOH. A series of experiments, including SEM (Scanning electron microscopy), FT-IR (Fourier transform infrared), XRD (X-ray diffraction), Raman, X-ray photoelectron spectroscopy (XPS), and N2adsorption/desorption, were carried out on the porous carbon produced from quinoa husk (PC–QH). The results showed that PC–QH was mainly composed of activated carbon and graphite. Moreover, PC–QH exhibited a high level of porosity with a BET (the Brunauer–Emmett–Teller theory) surface area of 1713 m2g−1. As a representative dye, malachite green (MG) was selected to evaluate the performance of PC–QH to absorb the contaminants in dyeing wastewater. In batch adsorption experiments, PC–QH exhibited a high adsorption rate toward malachite green (MG). An uptake capacity of 599.90 mg g−1was achieved in the initial 5 min, and the MG adsorption capacity of PC–QH reached 1365.10 mg g−1, which was higher than many other adsorbents. The adsorption data were well fitted with the Freundlich isotherm model and the pseudo-second-order kinetic model. PC–QH also displayed a high absorption rate to rhodamine B (RhB), methyl violet (MV), methylene blue (MB), and methyl orange (MO). The results in this study suggest that PC–QH can be a promising adsorbent for quick treatment of dyeing wastewater.Keywords:quinoa husk;porous carbon;adsorption;malachite green;water treatment
研究领域:
环境科学
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材料科学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）