Marine Life Science & Technology
·
2025
·
IF 5.3
Liu Kangjing,Tian Xueying,Fei Siyuan,Song Yukun,Abd El-Aty A. M.,Tan Mingqian
This study demonstrated the design of whey protein isolate (WPI)-mannose (Man) conjugates with triphenylphosphonium bromide (TPP) through self-assembly to prepare macrophage and mitochondrion dual-targeting astaxanthin (AXT) nanoparticles (AXT@TPP-WPI-Man). The nanoparticles displayed spherical structures with a well-dispersed size of approximately 206.1 ± 39.2 nm, with good biocompatibility, stability, and targeting capabilities. In vitro experiments demonstrated the specific accumulation of AXT@TPP-WPI-Man in mitochondria and exhibited good targeting ability toward macrophages. The AXT@TPP-WPI-Man effectively reduced reactive oxygen species and preserved the normal mitochondrial membrane potential. The AXT@TPP-WPI-Man treated ulcerative colitis mice exhibited a 52.32% increase in colon length with significant improvement in weight loss, disease activity index scores, and reduced release of inflammatory cytokines. Immunofluorescence staining indicated AXT@TPP-WPI-Man alleviated ulcerative colitis by reducing M1 polarization in colonic macrophages while promoting M2 polarization. The dual-targeting AXT@TPP-WPI-Man has the potential to improve astaxanthin bioavailability, presenting a promising delivery method for the treatment of ulcerative colitis.
研究领域:
生物医学工程
·
药理学
·
免疫学
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产品关联
DOI
PubMed
高影响因子
Aquaculture Reports
·
2022
·
IF 3.385
Jia Xu,Chaozhong Zheng,Shuyan Chi,Shuang Zhang,Junming Cao,Beiping Tan,Shiwei Xie
Dietary Clostridium autoethanogenum protein (CAP) has the potential to replace fishmeal (FM), but excessive CAP substitutions will affect gut health. Bile acids (BAs) have multiple benefits, but little information is available on the effects of BAs on Pacific white shrimp ( Litopenaeus vannamei ) fed a CAP diet (CAP replacing FM). An 8-week feeding trial were used to investigate the impacts of CAP substitution and BAs addition on structural changes of intestine, apoptosis, inflammatory cytokine and NFκB pathway of intestine, and transcriptomic profiles of hepatopancreas in Pacific white shrimp. Compared to PC (control) treatment, the NC (CAP50, CAP replacing 50% FM) treatment significantly decreased the fold height (FH) and villus length (VL) of midgut, and impaired the mitochondrial cristae. Compared to NC group, the FH was increased in BA1 (CAP50 with 0.03% BAs) and BA2 (CAP50 with 0.06% BAs) groups, the muscle layer thickness (MT) was decreased in BA2 and BA3 (CAP50 with 0.09% BAs) groups, the VL was increased in BA1 group, and the mitochondrial cristae was normal and well-defined in BA1 and BA2 groups (all p < 0.05). The NC increased the genes expression of apoptosis and NFκB signalling pathway, while the BA2 decreased the genes expression of apoptosis, inflammatory cytokine and NFκB signalling pathway. In transcriptomics analysis, a total of 171 differential expression genes (DEGs) were identified in NC group and 50 DEGs in BA2 group. There was 13 DEGs was observed both in NC and BA2 groups. The 5 KEGG pathways were statistically enriched in all treatments, including “Carbon metabolism”, “One carbon pool by folate”, “Glycine, serine and threonine metabolism”, “Amino sugar and nucleotide sugar metabolism”, and “PPAR signaling pathway”. In conclusion, CAP50 substitution impaired the intestinal health and 0.06% BAs addition improved it, both treatments altered the transcriptomics profiles of hepatopancreas in Pacific white shrimp.
研究领域:
水产养殖学
·
分子生物学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
Aquaculture Reports
·
2022
·
IF 3.385
Jia Xu,Xinzhou Yao,Yingying Lin,Shuyan Chi,Shuang Zhang,Junming Cao,Beiping Tan,Shiwei Xie
Dietary low-fishmeal (FM) have negative impacts on growth performance and metabolic responses in aquatic animals, while Schizochytrium limacinum have multiple benefits due to its rich contents of docosahexaenoic acid (DHA). However, little information is available on the effects of S. limacinum on Pacific white shrimp ( Litopenaeus vannamei ) in a low-FM diet (LF). An 8-week feeding trial were used to investigate the impacts of S. limacinum on growth performance, as well as morphological changes, biochemical indicators, antioxidant capacity, and transcriptomic profiles of hepatopancreas in shrimp fed a low-FM diet. Compared to LF diet, 0.3% S. limacinum (LFLD) diet significantly decreased the specific growth rate (SGR), and did not affect the feed conversion rate (FCR) and weight gain rate (WGR); whereas 0.6% S. limacinum (LFHD) diet did not affect the SGR, FCR and WGR. Histological structures of hepatopancreas showed that LFLD and LFHD treatments reduced the number and size of secretory cells and increased the number of storage cells, compared to LF group. LFLD and LFHD diets significantly decreased the concentrations of triglyceride and total cholesterol, significantly increased the levels of glutathione peroxidase and total antioxidative capacity, while did not affect the levels of malondialdehyde and superoxide dismutase. A total of 135 differential expression genes (DEGs) were identified in LFLD group and 108 DEGs were identified in LFHD group (both compared to LF group). Administration of S. limacinum altered the transcriptomic profiles, while LFHD induced a pattern similar to that of LF. LFLD and LFHD treatments mainly affected the 9 KEGG metabolic pathways, including “Arachidonic acid metabolism” and “Glutathione metabolism”. In conclusion, LFLD treatment impaired the growth performance of shrimp, while LFHD treatment did not affect it; and both treatments improved the morphological structure, lipid accumulation and antioxidant capacity of hepatopancreas.
研究领域:
水产养殖学
·
营养学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
MARINE POLLUTION BULLETIN
·
2020
·
IF 4.049
Lefei Jiao,Tianmeng Dai,Tinglan Cao,Min Jin,Peng Sun,Qicun Zhou
Heavy metal pollution arising from agricultural and industrial activities poses a significant threat to the aquatic environment, especially the increasing levels of chromium (Cr) that is exacerbating marine pollution. Given the economic importance of the Pacific white shrimp Litopenaeus vannamei ( L. vannamei ), understanding the impact of marine Cr pollution is deemed to be significant. In this study, we used the transcriptome sequencing (RNA-seq) technique to characterize the molecular mechanism of Cr exposure in L. vannamei . Gene ontology enrichment analysis showed substrate-specific and ion transport-related functions were mainly influenced by Cr exposure. We further identified genes involved in protein digestion and absorption (PEPT1, BAT1, MDU1), chemical carcinogenesis (GST and UGTs), ABC transporters (ABCC2), apoptosis (CAPN1, CASP10, PARP), implying the potentially Cr disintoxication mechanisms in L. vannamei . Genes within pancreatic secretion (ALT, LDH), lysosome (CTSL and HEXB), and peroxisome (ACOX1, ECI2, NUDT12) pathways implied the potentially Cr toxicity mechanisms in L. vannamei .
研究领域:
环境科学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2024
·
IF 7.6
Wanyu Zhang,Jian Liang,Sufeng Wang,Mingyang Lin,Jingshan Li,Li Chen,Yingchao Zhang,Tifeng Jiao
This study reported a new strategy for enhanced Pb 2+ and Cu 2+ sequestration by Artemia cyst shell (shell) supported nano-Mg from aqueous solutions and the carbonated exhausted-adsorbents sequenced potential application in photo-catalyst, which obtained two expected results. One is that the immobilization of nano-Mg onto Artemia cyst shell (shell-Mg) can greatly strengthen the adsorption effect of the neat cyst shell on Pb 2+ and Cu 2+ . The adsorption capacities of shell-Mg for both metal ions reached to 622.01 and 313.91 mg/g, which was 10–15 and 30–50 times that of the neat shell respectively. And shell-Mg has strong selectivity , which was approximately 2–4 times that of shell. The shell-Mg can be used to retrieve Pb 2+ and Cu 2+ from aqueous solutions efficiently. Another is that the carbonated exhausted-adsorbents (C-shell-Mg-Pb and C-shell-Mg-Cu) showed their potential photocatalytic degradation effects on congo red under pH = 4 condition, the decolorization rate reached to 61.19% and 80.39% respectively. Reuse of exhausted adsorbents can avoid the secondary pollution caused by the regeneration, extend the utilization value of exhausted adsorbents, and provide a new viewpoint for the reuse of spent bio-nanomaterial adsorbents.
研究领域:
环境科学
·
材料科学
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DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Xiaoting Yu,Xunyu Song,Yannan Chen,Yueqi Lv,Mingqian Tan
Astaxanthin (AXT) with mitochondria-targeted ability is desired for effectively preventing oxidative stress in various intestinal diseases. However, it is challenging to prepare mitochondria-targeted AXT (Mito-AXT) using traditional covalent bond methods due to the fragile structure of AXT and harsh reaction conditions, which result in low production yields and poor biocompatibility. Herein, we proposed a strategy to enhance the mitochondria-targeted efficiency of AXT by combining it with lipophilic cationic compounds through a non-covalent approach. Mito-AXT was synthesized through electrostatic coupling between anionic sulfonate-modified AXT (AXT-SO 3 ) and cationic (3-carboxypropyl) triphenylphosphonium bromide (TPP). The synthetic strategy successfully preserved the inherent DPPH and hydroxyl radical trapping capacity of AXT and achieved the efficient preparation of Mito-AXT with a yield of 86.17%. The accumulation of cationic TPP in mitochondria after the disassembly of Mito-AXT surprisingly facilitated the enrichment of anionic AXT-SO 3 in mitochondria. Notably, Mito-AXT regulated the redox balance, mitochondrial membrane potential, and mitophagy in IEC-6 cells under oxidative stress, thereby preventing apoptosis, compared to TPP-modified AXT molecules formed by covalent bonds. Furthermore, biosecurity analysis confirmed that Mito-AXT exhibited excellent biocompatibility. The results provided a simple, effective, and safe way to enhance the mitochondria-targeted efficiency of AXT through Mito-AXT, thereby effectively regulating the mitochondrial homeostasis in intestinal cells under oxidative stress.
研究领域:
生物化学
·
药理学
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Shan Sun,Dan Yang,Zheng Li,Wei Cheng,Yanli Yang,Ling Li,Xiangjie Luo,Yong Qian
Biogenic amines (BAs) are a class of nitrogen-containing natural organic compounds. Elevated levels of BAs are a reliable indicator of food spoilage and pose a significant risk to human health. Thus, the development of real-time sensors for BAs monitoring is crucial. In this study, we present a novel fluorescence probe, TP-A , based on ylidenemalononitrile enamine, which can detect various BAs including putrescine, cadaverine, and spermine. TP-A exhibits a significant fluorescence intensity increase at 600 nm in the presence of these BAs in an aqueous system, which can effectively monitor BAs in representative vegetable onion tissues. Notably, TP-A -loaded filter paper has been developed into test strips for real-time monitoring of BAs released from shrimp under different temperatures. This work offers a precise method for probing the release of BAs in real food samples, which is promising for application in food quality control.
研究领域:
食品科学
·
分析化学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 8
·1425
·
IF 5.1
Qiuyu Zhu,Lili Zhang,Xingming Sun,Baoguo Sun,Yuyu Zhang
Dried shrimp is a popular dietary ingredient that is often included in appetizer soups, stir-fry dishes, or other stews to improve the umami taste. The effects of adding dried shrimp on the sensory characteristics and taste components of sheep bone soup were investigated through sensory evaluation and untargeted approaches. The results of the single-factor and orthogonal experiments showed that the flavor qualities of sheep bone soup were optimal under the following conditions: 30% dried shrimp added, a 1:4.5 material–water ratio, and 2.7 h of stewing time. Sensory analysis showed a significant increase in the aroma, umami, kokumi, and texture intensity of the optimized sheep bone soup with dried shrimp. The untargeted approach combined with multivariate statistical analysis showed that compounds with a sweet taste (Lys and Ser), a umami taste and umami enhancement (Ala-Leu, Glu-Pro, Glu-Glu, Asp-Phe, pyroglutamic acid, and cinnamic acid), a bitter taste (Gly-Leu, Leu-Leu, Ile-Lys, and taurine), a kokumi taste (γ-Glu-Met, γ-Glu-Leu, γ-Glu-Ile, N-acetylmethionine, and N-acetylphenylalanine), a sour taste (malic acid), and a popcorn-like aroma (2-acetylthiazole) contributed significantly to the flavor enhancement of sheep bone soup. In addition, the contribution of Ac-Ser-Asp-Lys-Pro could not be ignored. These results contribute to a better understanding and improvement of the flavor qualities of sheep bone soup.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Xinmiao Wang,Dayong Zhou,Fujun Liu,Qian Wang,Chuan Li,Xu Guo,Peiyong Tong,Fawen Yin,Xiaoyang Liu,Jun Cao
Due to the fact that association colloids were formed in krill oil, the oxidation mechanism of krill oil was more complicated. In this study, water-soluble ferrous sulfate (Fe 2 (SO 4 ) 3 ), oil-soluble ferrous fumarate (C 54 H 99 FeO 6 ) and insoluble ferric oxide (Fe 3 O 4 ) were added to krill oil and stored at 60 °C for accelerated oxidation. Peroxide value, thiobarbituric acid reactive substances and aldehyde content showed that Fe 2 (SO 4 ) 3 had a stronger pro-oxidative effect. This was caused by the fact that water-soluble Fe 3+ was mainly accumulated in extremely high content at oil-water interface of association colloids. In contrast, oil-soluble C 54 H 99 FeO 6 was mainly distributed in the oil phase. Lipidomic analysis revealed that, phospholipids were rapidly catalytically decomposed by water-soluble Fe 3+ at the oil-water interface and produced lipid hydroperoxides and free radicals. Subsequently, the produced free radicals could enter the oil phase and set off a chain reaction of free radical formation, and then promote the oxidation of glycerides.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Shujie Wang,Yuyue Qin,Yaping Liu,Guoqin Liu,Guiguang Cheng,Thanapop Soteyome
In this study, three types of β -sitosterol-based oleogels ( β -sitosterol + γ -oryzanol oleogels, β -sitosterol + lecithin , oleogels and β -sitosterol + monostearate oleogels), loaded with astaxanthin, were employed as the oil phase to create oleogel-based emulsions (SO, SL, and SM) using high-pressure homogenization. The microstructure revealed that fine-scale crystals were dispersed within the oil phase of the droplets in the β -sitosterol oleogel-based emulsion. The bioaccessibility of astaxanthin was found to be 58.13 %, 51.24 %, 36.57 %, and 45.72 % for SM, SL, SO, and the control group, respectively. Interestingly, the release of fatty acids was positively correlated with the availability of astaxanthin (P = 0.981). Further analysis of FFAs release and kinetics indicated that the structural strength of the oil-phase in the emulsions influenced the degree and rate of lipolysis. Additionally, the micellar fraction analysis suggested that the nature and composition of the oleogelators in SM and SL also impacted lipolysis and the bioaccessibility of astaxanthin. Furthermore, interfacial binding of lipase and isothermal titration calorimetry (ITC) measurements revealed that the oleogel network within the oil phase of the emulsion acted as a physical barrier, hindering the interaction between lipase and lipid. Overall, β-sitosterol oleogel-based emulsions offer a versatile platform for delivering hydrophobic molecules, enhancing the bioavailability of active compounds, and achieving sustained release.
研究领域:
食品科学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
·
2024
·
IF 2.5
Shujie Cheng,Shimin Wu,Jianqiang Lan
Polycyclic aromatic hydrocarbons (PAHs) generated from heating butter pose a potential threat to consumer health. This study involved heating butter under different conditions and analysing the changes in the content of 54 PAHs. Furthermore, we investigated the inhibitory effects of β-carotene, pure astaxanthin and astaxanthin microcapsule powder on PAH formation. Results showed that heating temperature and duration affect the formation and transformation of PAHs, with higher temperatures facilitating the synthesis of oxygenated PAHs, whereas longer heating times promote the accumulation of light PAHs. Despite an observed increase in the concentration of light PAHs following heat treatment with added antioxidants, the antioxidants significantly reduced the toxic equivalency quotients (TEQs) in butter. This study revealed the PAH production pattern during butter heating and the effectiveness of antioxidants in inhibiting their accumulation.
研究领域:
食品科学
·
毒理学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Microbiology
·
2024
·
IF 4
Jia Jianping,Chen Zhitao,Li Qingqing,Li Feifei,Liu Siru,Bao Guoliang
Astaxanthin has multiple physiological functions and is applied widely. The yeast Phaffia rhodozyma is an ideal source of microbial astaxanthin. However, the stress conditions beneficial for astaxanthin synthesis often inhibit cell growth, leading to low productivity of astaxanthin in this yeast. In this study, 1 mg/L melatonin (MT) could increase the biomass, astaxanthin content, and yield in P. rhodozyma by 21.9, 93.9, and 139.1%, reaching 6.9 g/L, 0.3 mg/g DCW, and 2.2 mg/L, respectively. An RNA-seq-based transcriptomic analysis showed that MT could disturb the transcriptomic profile of P. rhodozyma cell. Furthermore, differentially expressed gene (DEG) analysis show that the genes induced or inhibited significantly by MT were mainly involved in astaxanthin synthesis, metabolite metabolism, substrate transportation, anti-stress, signal transduction, and transcription factor. A mechanism of MT regulating astaxanthin synthesis was proposed in this study. The mechanism is that MT entering the cell interacts with components of various signaling pathways or directly regulates their transcription levels. The altered signals are then transmitted to the transcription factors, which can regulate the expressions of a series of downstream genes as the DEGs. A zinc finger transcription factor gene (ZFTF), one of the most upregulated DEGs, induced by MT was selected to be overexpressed in P. rhodozyma. It was found that the biomass and astaxanthin synthesis of the transformant were further increased compared with those in MT-treatment condition. Combining MT-treatment and ZFTF overexpression in P. rhodozyma, the biomass, astaxanthin content, and yield were 8.6 g/L, 0.6 mg/g DCW, and 4.8 mg/L and increased by 52.1, 233.3, and 399.7% than those in the WT strain under MT-free condition. In this study, the synthesis and regulation theory of astaxanthin is deepened, and an efficient dual strategy for industrial production of microbial astaxanthin is proposed.
研究领域:
微生物学
·
生物化学
·
基因工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）