Frontiers of Materials Science
·
2023
·Vol. 17
·期 4
·1-11
·
IF 2.7
Zhang Hao,Luo Siyuan,Yang Weili,Luo Qisheng,Kannan Perumal Ramesh,Li Yao,Kong Xiangdong
Uncontrolled hemorrhage resulting from traumas causes severe health risks. There is an urgent need for expeditious hemostatic materials to treat bleeding incidents. Here, we developed a natural protein-based hemostatic sponge extracted from nonmulberry cocoons that exhibited rapid coagulation and effective absorption. We first built a degumming and dissolution system suitable for the Dictyoploca japonica cocoons to obtain regenerated silk fibroin (DSF). The DSF was then combined with carboxymethyl chitosan (CMCS) by glutaraldehyde (GA) crosslinking to ensure the structural stability of sponges. The resulting DSF–CMCS–GA exhibited remarkable hemostatic properties, displaying the highest absorption rate. It also demonstrated comparable efficacy to commercial hemostatic sponges. The blood-clotting index and hemolysis test showed that the prepared sponge possessed hemostatic activity and good hemocompatibility. Compared with mulberry silk fibroin hemostatic sponges (SF–CMCS–GA), DSF–CMCS–GA showed slightly better effects, making them a potential alternative to mulberry silk. In conclusion, our study introduces the use of Dictyoploca japonica silk fibroin for hemostasis, highlighting the exploitation of wild silkworm resources and providing an excellent silk fibroin-based hemostatic sealant for acute accident wounds and biomedical applications involving massive hemorrhage.
研究领域:
生物材料学
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医学工程
·
创伤治疗
✓ 已验证
DOI
高影响因子
Journal of Functional Foods
·
2025
·
IF 4
Zhixuan Xie,Luping Wang,Binqi Wang,Xueli Man,Yifei Wang,Taian Jin,Jiahui Ma,Eman Shawky,Samah Elsohafy,Tingfang Ma,Jianhua Li,Juan Jin,Qiang He,Jingkui Tian
Diabetic kidney disease (DKD), a prevalent microvascular complication affecting over 40 % of diabetes patients globally, imposes substantial burdens on healthcare systems through its dual impact on renal function deterioration and cardiovascular mortality. Silkworm pupae (SWP) is an ancient functional food documented in Chinese Materia Medica and Southeast Asian dietary practices, has recently emerged as a research focus due to its unique composition of chitin-derived peptides, antioxidant flavonoids, and angiotensin-converting enzyme inhibitory components. Building upon existing evidence of SWP's anti-inflammatory properties, this investigation evaluated the renoprotective effects of SWP in two representative stages—C2 (the fragile and soft stage) and C4 (nearly eclosion). The results indicated that SWP alleviated renal dysfunction and renal fibrosis through inhibiting apoptosis in DM mice. Notably, C2-stage extracts outperformed C4 counterparts in restoring podocyte integrity. These findings position SWP as a nutraceutical intervention for DKD management.
研究领域:
医学
·
营养学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2018
·Vol. 19
·期 3
·683
·
IF 3.687
Quan Gan,Xin Li,Xinwei Zhang,Lanlan Wu,Chongjun Ye,Ying Wang,Junshan Gao,Yan Meng
β-fructofuranosidase (β-FFase) belongs to the glycosyl-hydrolase family 32 (GH32), which can catalyze both the release of β-fructose from β-d-fructofuranoside substrates to hydrolyze sucrose and the synthesis of short-chain fructooligosaccharide (FOS).BmSuc1has been cloned and identified from the silkwormBombyx morias a first animal type of β-FFase encoding gene. It was hypothesized that BmSUC1 plays an important role in the silkworm-mulberry adaptation system. However, there is little information about the enzymatic core sites of BmSUC1. In this study, we mutated three amino acid residues (D63, D181, and E234) that represent important conserved motifs for β-FFase activity in GH32 to alanine respectively by using site-directed mutagenesis. Recombinant proteins of three mutants and wild type BmSUC1 were obtained by using a Bac-to-Bac/BmNPV expression system and BmN cells. Enzymatic activity, kinetic properties, and substrate specificity of the four proteins were analyzed. High Performance Liquid Chromatography (HPLC) was used to compare the hydrolyzing and transfructosylating activities between D181A and wtBmSUC1. Our results revealed that the D63A and E234A mutations lost activity, suggesting that D63 and E234 are key amino acid residues for BmSUC1 to function as an enzyme. The D181A mutation significantly enhanced both hydrolyzing and transfructosylating activities of BmSUC1, indicating that D181 may not be directly involved in catalyzation. The results provide insight into the chemical catalyzation mechanism of BmSUC1 inB. mori. Up-regulated transfructosylating activity of BmSUC1 could provide new ideas for usingB. moriβ-FFase to produce functional FOS.Keywords:Bombyx mori;β-fructofuranosidase;site-directed mutagenesis;hydrolysis;transfructosylation
研究领域:
酶学
·
分子生物学
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生物化学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 45
·28401-28409
·
IF 3.361
Boxiang Wang,Hangdan Xu,Jia Li,Dehong Cheng,Yanhua Lu,Li Liu
At present, Antheraea pernyi silk fibroin (ASF) based hydrogels have wide potential applications as biomaterials because of their superior cytocompatibility. Herein, ASF is used as a nucleophilic reagent, reacted with allyl glycidyl ether (AGE) for the preparation of allyl silk fibroin (ASF-AGE). The investigation of ASF-AGE structure by 1H NMR and FTIR are revealed that reactive allyl groups were obtained on ASF by nucleophilic substitution. A series of ASF based hydrogels are manufactured by N-isopropylacrylamide (NIPAAm) copolymerization bridged with ASF-AGE. By the silk fibroin self-assembly process, stably physical cross-linked hydrogels are formed without any crosslinking agent. These hydrogels exhibit good thermoresponsive and degradability, for which the LCST was about 32 °C, and these hydrogels can be degraded in protease XIV solution. Excellent cell proliferation, viability and morphology is demonstrated for b End.3 cells on the hydrogels by the characteristic MTT assay, CLSM and SEM. The cytocompatibility of b End.3 cells was demonstrated with excellent cell adhesion and growth on these ASF based hydrogels in vitro. These degradable and thermoresponsive ASF based hydrogels may find potential applications for cells delivery devices and tissue engineering.
研究领域:
生物材料学
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组织工程学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MATERIALS & DESIGN
·
2020
·
IF 6.289
Jianwei Liang,Xiaoning Zhang,Chi Yan,Yixuan Wang,Michael L. Norton,Xijun Wei,Carrie Donley,Yong Zhu,Peng Xiao,Yunhuai Zhang
As a key building block to power flexible electronics, there is need for the development of flexible current collectors. Bombyx mori silk, a traditional textile, attracts increasing attention for its potential application in flexible electronics and is a promising raw material for flexible carbon electrodes in current collector fabrication. In this work, molybdenum dioxide nanoparticles (MoO 2 NPs), which possess chemical stability and high mass specific capacitance, was used as a feed additive and combined with mulberry leaves to raise silkworms. It was found that the MoO 2 NPs were embedded within the silk fibers, which endowed the resulting silk with favorable features for electrochemical energy storage in flexible electronics. After carbonization, the specific capacitance of electrodes prepared with silk from the MoO 2 NPs feeding group, with a mass/volume ratio of 5 g/L, was enhanced from 100 F/g to 245 F/g at a current density of 0.2 A/g. Impressively, the electrodes show high coulombic efficiency with excellent stability. This work demonstrates the possibility of producing carbonized silk with superior properties for developing silk-based flexible electrodes for use in energy storage devices in a large-scale, green, low-cost way.
研究领域:
材料科学
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储能技术
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纳米技术
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纺织工程
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL SCIENCES
·
2019
·
IF 1.618
Ruru Chen,Lian Zhou,Hui Yang,Hailing Zheng,Yang Zhou,Zhiwen Hu,Bing Wang
The degradation behavior of silk fibroin (SF) is a significant and intriguing subject in the area of archaeological ancient silk research. In the present study, the immunological detection techniques combined with traditional characterization methods, jointly studied the degradation process of SF from Bombyx mori (B. mori) and Antheraea pernyi (A. pernyi) through exposure to alkaline proteinase, α-chymotrypsin, pepsin, and trypsin. Spectroscopic analysis revealed that different enzymes showed similar hydrolysis effects on the secondary structure, but the changes of B. mori SF and A. pernyi SF were mainly reflected in the decrease of β-sheet and the reduction of α-helical structure, respectively. In further research of immunology, two diagnostic antibodies were prepared corresponding to SF of B. mori and A. pernyi, respectively. The enzyme-linked immunosorbent assay (ELISA) and western blot indicated the enzyme-treated SF proteins still exhibited higher immunoreactivity because the epitopes on the surface of SF molecules are retained. Although α-chymotrypsin possesses the most cleavage sites among these enzymes, the α-chymotrypsin-treated SF did not exhibit significant changes in secondary structures and high antibody binding capacity. The results deepen our understanding of the SF degradation process during enzymatic hydrolysis, and show far-reaching guiding significance in trace detection of SF.
研究领域:
考古学
·
材料科学
·
生物化学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INSECT MOLECULAR BIOLOGY
·
2024
·
IF 2.3
Hui-Hua Cao,Wei-Wei Kong,Xi-Ya Chen,Sadaf Ayaz,Cai-Ping Hou,Yi-Sheng Wang,Shi-Huo Liu,Jia-ping Xu
The RNA interference pathway mediated by microRNAs (miRNAs) is one of the methods to defend against viruses in insects. Recent studies showed that miRNAs participate in viral infection by binding to target genes to regulate their expression. Here, we found that the Bombyx mori miRNA, miR-6498-5p was down-regulated, whereas its predicted target gene pyridoxal phosphate phosphatase PHOSPHO2 ( BmPLPP2 ) was up-regulated upon Bombyx mori nucleopolyhedrovirus (BmNPV) infection. Both in vivo and in vitro experiments showed that miR-6498-5p targets BmPLPP2 and suppresses its expression. Furthermore, we found miR-6498-5p inhibits BmNPV genomic DNA (gDNA) replication, whereas BmPLPP2 promotes BmNPV gDNA replication. As a pyridoxal phosphate (PLP) phosphatase (PLPP), the overexpression of BmPLPP2 results in a reduction of PLP content, whereas the knockdown of BmPLPP2 leads to an increase in PLP content. In addition, exogenous PLP suppresses the replication of BmNPV gDNA; in contrast, the PLP inhibitor 4-deoxypyridoxine facilitates BmNPV gDNA replication. Taken together, we concluded that miR-6498-5p has a potential anti-BmNPV role by down-regulating BmPLPP2 to modulate PLP content, but BmNPV induces miR-6498-5p down-regulation to promote its proliferation. Our findings provide valuable insights into the role of host miRNA in B. mori –BmNPV interaction. Furthermore, the identification of the antiviral molecule PLP offers a novel perspective on strategies for preventing and managing viral infection in sericulture.
研究领域:
分子生物学
·
病毒学
·
昆虫学
·
RNA干扰
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Richard Ansah Herman,Zhan-Peng Zhang,Marriam Khurshid,Ellen Ayepa,Cheng-Hai Yan,Jacob Paarechuga Anankware,Jun Wang
The gut microbiota of the silkworm ( Bombyx mori ) is essential for metabolic processes, including digestion and immunity. Nonetheless, the role of specific gut fungi in optimizing nutrient recycling influenced by infiltrated biomaterials remains inadequately elucidated. This study investigates the potential mechanisms through which gut fungi affect the dynamics of nutrient absorption in silkworms. For this purpose, 5th instar silkworms were exposed to Fe 3 O 4 -urease nanoconjugates for 168 h following the utilization of high-throughput microbiome sequencing to identify shifts in principal fungal groups. The conditional fungi groups Mucoromycota and Basidiomycota significantly increased from 10.28 % to 47.16 % and 0.53 % to 2.63 % respectively ( p < 0.05), while Ascomycota decreased from 86.57 % to 52.29 %, having no negative impact on the growth and sustainability of the host insect. Functional analysis using FunGuild showed enriched trophic nodes including pathotrophs, saprotrophs and symbiotrophs while functions of DEGs demonstrated an increased metabolic capacity associated with iron and heme binding, and lipase activity. This reveals significant restructuring of the gut fungi microbiota with specific fungal taxa exhibiting enhanced abundance and diversity correlated with the presence of Fe 3 O 4 -urease nanoconjugates, serving as a potential for engineered nanomaterials to promote the sustainability and development of sericulture.
研究领域:
微生物学
·
分子生物学
·
昆虫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Yue Ming,Zhuangzhuang Shi,Yufei Zhao,Jue Li,Wei Sha,Weilong Wang,Guoqiang Li,Mengmeng Wu,Ting Ma
The oral delivery of probiotics faces numerous challenges, particularly the acidic gastric environment. This study aimed to construct microcapsules using acid-resistant polysaccharide sanxan gum (SG) combined with sodium alginate (SA) and chitosan (CS), and to evaluate their protective effects on the probiotic bacteria Lactobacillus plantarum ( Lp ) during in vitro simulated gastrointestinal digestion. Sanxan gum, an edible polysaccharide produced by Sphingomonas sanxanigenens strain, is composed of mannose, glucuronic acid, rhamnose, and glucose. Sodium alginate has been widely used to protect probiotics because it is cheap, safe, and easy to form into microcapsules. Chitosan is a cationic polysaccharide with excellent mucoadhesive properties and film-forming capabilities. Herein, the microcapsules were prepared using the extrusion drop method. Compared with microcapsules based solely on sodium alginate, those with a ratio of sanxan gum to sodium alginate of 4:6 demonstrated enhanced encapsulation efficiency (97.66 ± 0.79 %) and freeze-dried cell viability (9.97 ± 0.03 log CFU/g). Furthermore, the incorporation of chitosan yielded the highest encapsulation efficiency for the Lp @SG-SA@CS microcapsules (99.14 ± 1.46 %) and significantly enhanced their tolerance to heat, bile salts, and gastric acid. Our findings suggest that sanxan gum can confer acid resistance to microcapsules, highlighting its potential for applications in diverse acidic environments.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2025
·
IF 5.4
Di Mei,Na Li,Kun Yan,Jiannan Wang,Xiufang Li,Renchuan You,Dong Wang
Nature organizes the extracellular matrix into hierarchical structures, inspiring the design of biomimetic mineralization scaffolds. Silk fibroin, a noncollagenous structural protein, is a potential template for biomineralization. Here, we show the mineralization behavior of silk nanofibril (SNF) and demonstrate tunable biomineralization through the SNF assembly. We demonstrate the deposition of amorphous calcium phosphate and its transformation into flower-like hydroxyapatite crystals on the SNF interface by exploiting individual SNFs as a model. On two-dimensional (2D) films and three-dimensional (3D) scaffolds, SNF assembly influences the mineralization process. The dense nanofibrils in the SNF film and scaffold undergo mineralization through the anchoring and deposition of hydroxyapatite, whereas the modified 3D scaffolds with fluffy SNF are comparable to individual SNF. Consequently, silk nanotechnology makes use of a controllable assembly to build one-dimensional, 2D, and 3D nanostructures. These results offer new insights into the mineralization mechanisms of silk mesoscopic units and provide pathways for the synthesis of biomineralized scaffolds.
研究领域:
生物材料
·
纳米技术
·
组织工程
·
生物矿化
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FISH PHYSIOLOGY AND BIOCHEMISTRY
·
2024
·Vol. 50
·期 3
·1157-1169
·
IF 2.5
Guo Haoji,Liu Guoquan,Tian Mengdu,Liu Chunsheng,Zhang Haoran,Wang Shuqi,Wen Xiaobo,Lin Fan
Faba bean has gained attention as a cost-effective protein source with the potential to enhance product quality (texture properties, collagen content, etc.) in fish. However, its anti-nutrition factor, high feed conversion ratio, poor growth performance, etc. limit the widely application as a dietary source, especially in carnivorous fish. The water or alcohol extract of faba bean might resolve the problem. In this study, the juvenile Nibea coibor , known for their high-protein, large-sized, and high-grade swim bladder, were fed with seven isoproteic and isolipid experimental diets with the additive of faba bean water extract (1.25%, 2.5%, and 5%) or faba bean alcohol extract (0.9%, 1.8%, and 3.6%), with a control group without faba bean extract. After the 10-week feeding trail, the growth, antioxidant capacity, textural properties, and collagen deposition of the swim bladder were analyzed. Results showed that the 1.25% faba bean water extract group could significantly promote growth, textural quality of the swim bladder, and have beneficial effects on antioxidant response of fish. Conversely, dietary supplementation of faba bean alcohol extract resulted in reduced growth performance in a dose-dependent manner. Furthermore, fish fed diet with 1.25% faba bean water extract exhibited increased collagen content and upregulated collagen-related gene expression in the swim bladder, which was consistent with the Masson stain analysis for collagen fiber. Our results suggested that the anti-nutrient factor and bioactive component of faba bean may mainly be enriched in alcohol extract and water extract of faba bean, respectively. Besides, the appropriate addition of water extract of faba bean may improve the texture quality of the swim bladder by promoting collagen deposition. This study would provide a theoretical basis for the formulated diets with faba bean extract to promote product quality of marine fish.
研究领域:
水产养殖学
·
营养学
·
食品科学
✓ 已验证
DOI
高影响因子
ARCHAEOMETRY
·
2019
·Vol. 61
·期 4
·921-932
·
IF 1.64
H. L. Zheng,C. Ma,X. J. Dai,Y. Zhou
Identifying the traces and residues of unearthed silk fibres is crucial for further analysis and study. In this study, an enzyme-linked immunosorbent assay (ELISA) based on the monoclonal antibodies specific to Bombyx mori fibroin was developed in order to for detect ancient silk. Five monoclonal antibodies were successfully prepared based on fibroin hydrolysate, and four had a high specificity for mulberry silk. Furthermore, they could be used to discern B. mori fibre from tussah fibre and eri fibre. The ELISA standard curve of the most sensitive monoclonal antibody, anti-Silk Fibroin (SF)-3, was y = 0.022 x + 0.177 ( R 2 = 0.998), and the detection limit was 4.65 ng/ml. Using this antibody, we successfully identified five ancient mulberry silk samples from different time periods. The results suggest that ELISA assays based on monoclonal antibodies can be used as an immunological testing method for unearthed silk fibre.
研究领域:
考古学
·
免疫学
·
材料科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）