SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Zhigang Yang,Liangliang Zhu,Jiani Liu,Yongxu Cheng,Khor Waiho,Aqin Chen,Youji Wang
Microplastics may be potential vectors for environmental contaminants such as heavy metals in the aquatic ecosystem due to their highly hydrophobic surfaces and fugacity property. To investigate the combined effects of microplastics with Pb, we exposed juvenile Chinese mitten crabs Eriocheir sinensis to different Pb concentrations (0, 5 and 50 μg/L) combined with microplastics (0 and 400 μg/L) for 21 days to determine the Pb bioaccumulation, oxidative stress, lipid anabolism, and histopathology of hepatopancreas. In general, the results showed that compared to single Pb exposure, the combination of MPs and Pb significantly increased the bioaccumulation of Pb, activities/content of antioxidant biomarkers and lipid metabolism enzymes, and liver injury parameters in crabs, indicating MPs are potential vector of heavy metals and co-exposure exerts more severe effects on crabs. This study provides the insights into the oxidative defense and preliminary lipid anabolism of economic crustaceans in response to combined stress of Pb and MPs.
研究领域:
环境科学
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水生毒理学
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生态毒理学
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DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2023
·
IF 4.7
Ju Li,Jinmei Zuo,Xiaoyan Lv,Jiahe Ma,Xiaohong Li,Simiao Fu,Jinsheng Sun
Tissue autotomy is a unique adaptive response to environmental stress, followed by regeneration process compensating for the loss of body parts. The crustaceans present remarkable activity of appendage autotomy and regeneration, however, the molecular mechanism is still unclear. In this study, the Eriocheir sinensis Hedgehog ( EsHH ) and Smoothened ( EsSMO ) were identified in the regenerative limbs, and the function of Hedgehog signaling pathway on limb regeneration was evaluated. At the blastema growth stage of limb regeneration, the expression of EsHH and EsSMO was up-regulated in response to limb autotomy stress, and down-regulated at blastema differentiation stage. To clarify the effect of Hedgehog pathway during limb regeneration, the regenerative efficiency was evaluated with Smoothened inhibitor cyclopamine or RNAi ( ds-HH ) injection. We observed that the regenerative efficiency was significantly repressed with blockage of Hedgehog pathway at both the basal growth stage and the proecdysial growth stage, which was indicated by the delay of wound healing and blastema growth, as well as a decrease in the size of newly formed limbs. In addition, gene expression and BrdU incorporation assay showed that the proliferation and myogenic differentiation of blastema cells were suppressed with either cyclopamine or ds- HH injection. Thus, these results suggest that Hedgehog signaling pathway is essential for the establishment of limb regeneration in E. sinensis through promoting the proliferation and myogenic differentiation of blastema cells.
研究领域:
发育生物学
·
分子生物学
·
再生医学
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DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Rong Shi,Zhongqi Sun,Xuemei Yu,Huamao Wei,Gaoshang Li,Wenge Yang
The objective of this study was to examine the impact of magnetic field-assisted thawing (MFT) on the quality and microstructure of Portunus trituberculatus meat, in comparison to other thawing methods (Air thawing, AT; Hydrostatic thawing, HT; Microwave thawing, MT). A comprehensive comparative analysis was conducted on different physicochemical and flavor properties of crab meat. MFT significantly reduced thawing loss by 18.9–20.0 % and TBARS levels by 40 % compared to the other methods, demonstrating its ability to minimize lipid oxidation. Microstructure analysis showed that MFT preserved muscle fiber integrity, leading to a denser and more organized structure with reduced protein denaturation. This performance was attributed to MFT’s ability to reduce protein denaturation, which effectively minimizes water loss and inhibits lipid oxidation during thawing. Moreover, MFT improved the retention of key flavor compounds, increasing nonanal and 2-undecanone levels by 31.4 % and 69 %, respectively, contributing to a fresher aroma. MFT group also retained more umami (Glu + 40.8 %) and sweet amino acids (Gly, Ala + about 19.8 %), and nucleotides (AMP + 0.8 %, IMP + 9.4 %), while less bitter amino acids (Lys −68.2 %, Met −51.9 %). In conclusion, MFT had good ability of keeping quality of crabs meat, providing noval thawing method for P. trituberculatus .
研究领域:
食品科学
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水产养殖
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生物化学
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热能工程
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Zhiruo Fang,Yu Yao,Liping Cao,Jiancao Gao,Quanjie Li,Zhijuan Nie,Yi Sun,Gangchun Xu,Jinliang Du
This study investigated the toxicological mechanism of deltamethrin on Chinese mitten crab Eriocheir sinensis juveniles in fresh water. We first conducted an acute toxicity test, followed by laboratory methods to detect changes in immune-related indices in terms of antioxidant enzyme markers, lipid metabolism-related genes, and autophagy-related and apoptosis genes. The acute toxicity (96-h LC50) of deltamethrin to E. sinensis was 7.195 μg/L. After 48 h of exposure, serum showed elevated immune-related indices ( P < 0.05) for alkaline phosphatase (AKP), acid phosphatase (ACP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), complement components C3 and C4, and the key pro-inflammatory cytokines interleukin-6, interleukin-1β, and tumor necrosis factor alpha (TNF-α). In hepatopancreas at 48 h, indicators related to the antioxidant system, namely superoxide dismutase (SOD) and glutathione (GSH), were significantly elevated, whereas nitric oxide and total antioxidant capacity (T-AOC) were decreased ( P < 0.05). In contrast, lipid metabolism indices for triglyceride (TG), total cholesterol (TC), and malondialdehyde (MDA) were increased ( P < 0.05). Transcriptomics and metabolomics revealed that exposure to deltamethrin disrupted the lipid metabolic process in the hepatopancreas mainly by altering fatty acid synthesis, amino acid metabolism, immune signaling, and autophagy activation, while the exposure increased the content of phospholipids and cholesterol but decreased the levels of amino acids and palmitoleic acid. Quantitative genetics revealed significantly aberrantly expressed ( P < 0.05) lipid metabolism-related genes, including acc1 , fasn , scd1 , and pnpla2 , all key genes involved in lipid accumulation. Deltamethrin exposure also significantly altered ( P < 0.05) gene expression levels for Toll-like receptor ( tlr ), myeloid differentiation factor 88 ( myd88 ), crustin1 , anti-lipopolysaccharide factor isoform 3 ( alf3 ), tumor necrosis factor alpha ( tnf-α ), and NF-κB transcription factor relish . Furthermore, deltamethrin activated the toll-like receptor/major myeloid differentiation response gene 88/nuclear factor kappa-light-chain-enhancer of activated B cells (TLR/MyD88/NF-kB) signaling pathway, which activates a nonspecific immune response in E. sinensis . Additionally, carnitine palmitoyltransferase 1 A ( cpt1a ), cytochrome c ( cyt-c ), adenosine 5′-monophosphate (amp)-activated protein kinase ( ampk ), the autophagosomal protein microtubule-associated protein 1 light chain 3c ( lc3c ), and the autophagy-related proteins beclin1 , atg5 , atg12 were significantly induced ( P < 0.05) in the adenosine monophosphate-activated protein kinase/rapamycin (AMPK/mTOR) signaling pathway. These changes resulted in excess free radicals, causing oxidative stress in the mitochondrial membrane, promoting mitochondrial autophagy. The results confirm that deltamethrin exposure can induce hepatopancreatic injury by promoting mitochondrial autophagy, activating an immune response, and inhibiting lipid metabolism. Overall, this study provides multi-level information to reveal the toxic effects of deltamethrin on E. sinensis .
研究领域:
毒理学
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分子生物学
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环境科学
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水生生物学
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DOI
PubMed
高影响因子
STROKE
·
2024
·
IF 7.8
Zimu Jiang,Jiating Wei,Jiahui Liang,Weixian Huang,Fubing Ouyang,Chunyong Chen,Pingping Li,Suhan Cao,Yuangui Cai,Jianle Li,Baozi Huang,Jinsheng Zeng,Yicong Chen
BACKGROUND:Remote secondary neurodegeneration is associated with poststroke cognitive impairment (PSCI). Dl-3-n-butylphthalide (NBP) improves PSCI clinically. However, whether it ameliorates PSCI by alleviating secondary neurodegeneration remains uncertain. Nonhuman primates provide more relevant models than rodents for human stroke and PSCI. This study investigated the effects of NBP on PSCI and secondary neurodegeneration in cynomolgus monkeys after permanent left middle cerebral artery occlusion (MCAO).METHODS:Thirteen adult male cynomolgus monkeys were randomly assigned to sham (n=4), MCAO+placebo (n=5), and MCAO+NBP groups (n=4). The MCAO+placebo and MCAO+NBP groups received saline and NBP injections intravenously, respectively, starting at 6-hour postsurgery for 2 weeks, followed by soybean oil and NBP orally, respectively, for 10 weeks after MCAO. Infarct size was assessed at week 4 by magnetic resonance imaging. Working memory and executive function were evaluated dynamically using the delayed response task and object retrieval detour task, respectively. Neuron loss, glia proliferation, and neuroinflammation in the ipsilateral dorsal lateral prefrontal cortex, thalamus, and hippocampus were analyzed by immunostaining 12 weeks after MCAO.RESULTS:Infarcts were located in the left middle cerebral artery region, apart from the ipsilateral dorsal lateral prefrontal cortex, thalamus, or hippocampus, with no significant difference between the MCAO+placebo and MCAO+NBP group. Higher success in delayed response task was achieved at weeks 4, 8, and 12 after NBP compared with placebo treatments (P<0.05), but not in the object retrieval detour task (all P>0.05). More neurons and less microglia, astrocytes, CD68-positive microglia, tumor necrosis factor-α, and inducible NO synthase were observed in the ipsilateral dorsal lateral prefrontal cortex and thalamus after 12 weeks of NBP treatment (P<0.05), but not in the hippocampus (P>0.05).CONCLUSIONS:Our findings indicate that NBP improves working memory by alleviating remote secondary neurodegeneration and neuroinflammation in the ipsilateral dorsal lateral prefrontal cortex and thalamus after MCAO in cynomolgus monkeys.
研究领域:
神经科学
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药理学
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认知科学
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神经病学
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产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Yuean Chu,Mingyue Yu,Yunxing Li,Suoxian Yang,Hongyan Bi
Tracement of the origin of seafoods and aquatic products is an important issue for avoiding the falsification of production regions of aquatic products. Herein, by analyzing black tiger shrimp ( Penaeus monodon ) as a model of aquatic products, we demonstrated a method to trace the origin of aquatic products by analyzing the gut microbiome of black tiger shrimp ( Penaeus monodon ) collected from three commercial farms in China, via matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis. Multivariate analysis was executed on the obtained mass spectrometric datasets of bacteria extracted and collected from shrimp samples with different production regions. The gut microorganisms of black tiger shrimps can be identified by scoring the gut microbiota of black tiger shrimps collected from three producing areas. The black tiger shrimp from three production regions were found with distinguished characteristics of bacterial prevalence in their digestive gland microbiota. The dominant bacteria genus in digestive gland microbiota from black tiger shrimp samples from the three production regions are completely different. Single-blind shrimp samples with unknown production region was successfully screened by comparing the detected MS data in different ways. This strategy can trace the production region of the analyzed shrimp samples in a quick and efficient mode. The application of the present protocol was further demonstrated by analyzing the biometric species from gill tissue of Chinese mitten crab ( Eriocheir sinensis ) samples collected from crab-producing areas in Jiangsu Province (China), that is, Taihu Lake, Gaoyou Lake, and Hongze Lake for tracing their production area. It is expected to extend the presently developed method to provide clues for the origin traceability of other aquatic products.
研究领域:
水产养殖
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食品科学
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微生物学
·
质谱分析
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Wenya Shi,Jie Yang,Yuyang Guo,Zhaoyu Zhang,Hualin Li,Han Guo,Jianqiu Deng,Qingrong Yao,Lichun Cheng,Qianxin Long
Industrial wastewater containing heavy metal ions has become an urgent problem to be solved due to its environmental persistence and bioaccumulation. In this study, powdered adsorbent (DE/CBC) and spherical adsorbent (SA@DE/CBC) were prepared from natural diatomite (DE), crab shell biochar (CBC) and sodium alginate (SA) as raw materials, and were used to adsorb Pb(II) from wastewater. The adsorption isotherm fitting showed that the experimental process of DE/CBC and SA@DE/CBC conformed to the Langmuir model, and the maximum adsorption capacity of the monolayer reached 1155 mg/g and 365 mg/g, respectively. The pseudo-first-order kinetic model showed better fitting for the two adsorbents, and the diffusion rate was controlled by multiple steps. Compared with most traditional composite adsorbents, DE/CBC and SA@DE/CBC can achieve 98 % and 93 % adsorption rates at relatively high Pb(II) concentrations, and maintain 83 % and 84 % high adsorption capacity after 10 adsorption–desorption cycles, demonstrating superior Pb(II) adsorption performance and excellent structural stability. The spherical morphology not only effectively solves the problems of adsorbent recovery and secondary pollution prevention and control, but also plays a role in protecting the structure and active sites of powdery materials, making it more durable and practical. Collectively, the developed composite not only enables high-value-added utilization of biowaste but also demonstrates cost-effectiveness and excellent reusability, providing a sustainable remediation strategy for lead-contaminated water systems.
研究领域:
环境科学
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材料工程
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化学工程
·
水处理技术
·
吸附过程
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产品关联
DOI
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Tianyu Wang,Hang Lu,Yuanyong Tian,Hui Zhao,Xuefeng Lu,Zhaoxia Wu,Shiwei Niu
Ammonia accumulation in aquaculture systems affects the sensory quality of Eriocheir sinensis , and mechanisms linking ammonia to aroma changes remain unclear. This study investigated how ammonia exposure in aquatic environments (0, 10.47, and 41.87 mg/L total ammonia-N, 48 h) alters aroma profiles of E. sinensis meat. Using HS-SPME-GC–MS and HS-GC-IMS, we identified 26 key volatile organic compounds (VOCs) defining aroma differences. Concurrently, fatty acid profiles were analyzed through GC–MS. Ammonia exposure reduced fishy (trimethylamine, 1-octen-3-ol) and meaty (methional) aromas while enhancing plant-like (phenylethyl alcohol) and nutty (2-methylbutanal) aromas in the low-concentration group; high-concentration ammonia diminished hexanal and sulfur-containing compounds, aligning with aroma evaluation trends. Further, n-3 polyunsaturated fatty acids increased after ammonia exposure, correlating with VOCs derived from lipid metabolism. These findings reveal ammonia-induced metabolic changes in flavor precursors (e.g., fatty acids) and flavor compounds (e.g., VOCs) and provide feasible insights for optimizing aquaculture practices to improve crab quality.
研究领域:
水产养殖
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食品科学
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分析化学
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环境毒理学
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风味化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
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IF 5.162
Kulang Primo Sokiri Kiliona,Mengqin Zhou,Yan Zhu,Ping Lan,Ning Lin
Incompatibility of chitin nanomaterials with organic solvents is challenging in the design of the desirable organogels. The long hydrocarbon chains were covalently grafted on the surface of nanochitins, with the attachment of reactive allyl groups and improved dispersion in organic solvents. The reactive thiol groups of poly (ethylene glycol) were introduced into the allyl-nanochitin suspensions to produce the organogels by the thiol-ene click reaction. Attributed to the UV-induced cross-linking between the soft segments of thiolated−PEG and the allyl-nanochitin, the stable organogels with the storage modulus higher than the loss modulus by one order of magnitude were obtained, exhibiting the significant phase transition and mechanical enhancement on the rheological behavior. The combination of crystalline allyl-nanochitin and polymeric chains played a crucial role in the construction of the micro-network, attributing to the stability and mechanical strength of the organogels.
研究领域:
材料科学
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化学工程
·
高分子化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Ye Yuan,Fangmin Xu,Min Jin,Xuexi Wang,Xiaoying Hu,Mingming Zhao,Xin Cheng,Jiaxiang Luo,Lefei Jiao,Mónica B. Betancor,Douglas R. Tocher,Qicun Zhou
While tissue fatty acid compositions reflect that of the dietary lipid source, little information is available on how dietary oils modify lipid class and molecular species profiles in hepatopancreas of crustacean. Herein, an 8-week nutritional trial and untargeted lipidomic analysis were used to investigate the impacts of dietary n-3 PUFA lipid sources including fish oil, krill oil and linseed oil on the lipidomic characteristics of hepatopancreas of swimming crab ( Portunus trituberculatus ). Dietary krill oil significantly increased distribution of 20:5n-3 and 22:6n-3 at sn-2 in phosphatidylcholine and phosphatidylethanolamine compared to fish oil. Fish oil intake promoted the deposition of 20:5n-3 and 22:6n-3 at sn-1,2,3 in triglyceride compared to linseed oil, which significantly increased the specific accumulation of 18:3n-3 at sn-1,3 in triglyceride and sn-2 in phosphatidylcholine and phosphatidylethanolamine. The study revealed metabolic responses to different dietary n-3 PUFA in swimming crab, which provided novel insight into the lipid nutrition of crustacean.
研究领域:
水产养殖
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营养学
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脂质代谢
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2018
·Vol. 410
·期 7
·2011-2018
·
IF 3.307
Yu Lingling,Wen Chao,Li Xing,Fang Shiqi,Yang Lichuan,Wang Tony,Hu Kaifeng
Quantification of endogenous and exogenous plasma glucose can help more comprehensively evaluate the glucose metabolic status. A ratio-based approach using isotope dilution liquid chromatography tandem mass spectrometry (ID LC-MS/MS) with indirect multiple reaction monitoring (MRM) of the derivative tag was developed to simultaneously quantify endo-/exogenous plasma glucose. Using diluted D-[ 13 C 6 ] glucose as tracer of exogenous glucose, 12 C 6 / 13 C 6 glucoses were first derivatized and then data were acquired in MRM mode. The metabolism of exogenous glucose can be tracked and the concentration ratio of endo/exo-genous glucose can be measured by calculating the endo-/exo-genous glucose concentrations from peak area ratio of specific daughter ions. Joint application of selective derivatization and MRM analysis not only improves the sensitivity but also minimizes the interference from the background of plasma, which warrants the accuracy and reproducibility. Good agreement between the theoretical and calculated concentration ratios was obtained with a linear correlation coefficient ( R ) of 0.9969 in the range of D-glucose from 0.5 to 20.0 mM, which covers the healthy and diabetic physiological scenarios. Satisfactory reproducibility was obtained by evaluation of the intra- and inter-day precisions with relative standard deviations (RSDs) less than 5.16%, and relative recoveries of 85.96 to 95.92% were obtained at low, medium, and high concentration, respectively. The method was successfully applied to simultaneous determination of the endo-/exogenous glucose concentration in plasma of non-diabetic and type II diabetic cynomolgus monkeys.
Graphical Abstract The scheme of the proposed ratio-based approach using isotope dilution LC-MS/MS with indirect MRM of the derivative tag for simultaneous quantification of endogenous and exogenous plasma glucose.
研究领域:
分析化学
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代谢组学
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生物医学科学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2022
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IF 10.723
Yi Sun,Wei Zhang,Shan Lu,Wenhua Miao,Meiling Chen,Fei Liu,Haining Na,Jin Zhu
Herein, a novel cellulose-based fluorescent material with amine-responsive property was developed in a CO 2 switchable solvent for the contactless, real-time, and visual monitoring of the freshness of shrimp , crab, and fish. A nucleophilic cellulose carbonate intermediate is in-situ formed upon dissolution of cellulose in the CO 2 switchable solvent, which is adopted for the highly effective grafting of poly( l -lactic acid) and amine-responsive fluorescent reagent 7-amino-4-(trifluoromethyl) coumarin onto the cellulose backbone via ring opening polymerization and nucleophilic substitution reaction, respectively. The product has good processibility, and sensitive and reversible fluorescent responsive towards ammonia. The fluorescent intensity of the material decreased significantly and its fluorescent color changed from blue to cyan, when the aquatic products got spoiled. This study indicates that the CO 2 switchable solvent has great potential for the modification of cellulose with various functionality by taking advantage of the highly active nucleophilic cellulose carbonate intermediate.
研究领域:
材料科学
·
化学工程
·
食品科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）