LWT-FOOD SCIENCE AND TECHNOLOGY
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2019
·
IF 3.714
Dan Zhou,Qinlong Shi,Jiangbo Pan,Min Liu,Yi Long,Fahuan Ge
ion In this study, the combination of supercritical fluid extraction and molecular distillation (SFE-MD) was used to improve the quality of camellia oil. A series of important process parameters were investigated to obtain high quality camellia oil. Compared with the camellia oil produced by supercritical fluid extraction (SFE), the acid value of camellia oil obtained by SFE-MD was significantly decreased to 0.63 ± 0.01 from 2.93 ± 0.01 mg KOH/g oil, without any change of their fatty acid compositions. Furthermore, unsaponifiable matters of camellia oil such as γ-tocopherol, β-sitosterol and squalene could be effectively preserved and there were no residual harmful substances. The technology provided in this study is effective, economical and environment-friendly and could be replicated in an industrial scale.
研究领域:
食品科学
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化学工程
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植物提取
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Zihan Xu,Shanshan Liu,Mingyue Shen,Jianhua Xie,Jun Yang
Trans fatty acids (TFAs), associated with the risks of coronary heart disease and diabetes, are formed by isomerization of unsaturated fatty acids during refining of linseed oils. In this study, TFAs and the chemical characteristics (acid value, peroxide value, carbonyl compounds, bioactive minor components and fatty acids) in 32 commercial linseed oils were investigated, and the correlation among them were further analyzed. Results showed that C18:3 TFAs were predominant TFAs in linseed oils and about 9% of the samples had TFA contents above 2 g/100 g fat, as well as the average level of TFA in the refined samples was higher than that in the unrefined oils. The correlation analyses suggested C18:3 TFAs exhibited significant negative correlations with acid value, levels of acetone, trans -2-nonenal, campesterol and α-linolenic acid. These results provided a comprehensive insight of TFAs in linseed oil and had important implications for consumers and linseed oil industry.
研究领域:
食品科学
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营养学
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化学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Chi Zhang,Zhinan Fan,Hao Zhan,Hong Zhou,Rongliang Ma,Li-Juan Fan
Both latent sebaceous and blood fingerprints may provide valuable information for forensic investigation. To detect both types of fingerprints with no need to predistinguish them, a new adaptive developing strategy was proposed. A cationic conjugated polymer with poly[p-(phenylene ethylene)-alt-(thienylene ethynylene)] backbone (PPETE-NMe3+) was synthesized, which was dissolved in N,N-dimethylformamide (DMF) to form the developing solution. Fingerprints were developed by a simple dropping and incubating process without any pre-/post-treatments. Fluorescent photographs of the developed fingerprints on various substrates demonstrated that this developing strategy was effective for both types of fingerprints on nonporous substrates. Gray value analysis further confirmed the enhancement of the legibility of the fingerprint images. The preliminary mechanism exploration suggested that certain weak interactions, such as hydrophobic interaction and electrostatic interaction, may synergistically contribute to the interaction between the polymer and fingerprint components. The molecular design of the polymer combined with an appropriate solvent endowed the developing system the adaptiveness toward different types of fingerprints. This adaptive developing strategy made the fingerprint-developing process more efficient and may be further extended to more practical application scenes.
研究领域:
法医学
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聚合物化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOTECHNOLOGY
·
2018
·
IF 2.533
Ming Zhao,Shui-Ling Wang,Xin-Yi Tao,Guo-Ling Zhao,Yu-Hong Ren,Feng-Qing Wang,Dong-Zhi Wei
Due to the lack of powerful gene regulation elements, the engineering development of Streptomyces is often limited. Here, we disclosed that the heterologous σ 70 -dependent promoters, which have been reported as inefficient tools for gene expression in Streptomyces , could be efficiently recognized by Streptomyces housekeeping factor σ hrdB . Therefore, an effective strategy was developed to engineer these promoters for robust gene expression in Streptomyces by fusing them with optimized 5′-untranslation regions (5′-UTRs). As a proof of concept, the widely used P tac in E. coli was engineered by fusing its core promoter region with the 5′-UTR R15 from a relatively powerful Streptomyces promoter P kasO * R15 and resulted in P tac* , the activity of which was 8.1-fold that of P tac and 1.7-fold that of P kasO * R15 in S. lividans TK24. Next, the 5′-UTR R15 was optimized by randomizing the ribosome binding site (RBS). Based on the base biases of those RBSs with higher activity, eight artificial RBSs were rationally designed, and the optimal resulting promoter P tac * RBS3 showed about 2.1, 3.6, and 17.6 times the activity of P tac *, P kasO * R15 , and P tac , respectively, demonstrating that the heterologous P tac was converted into a type of robust Streptomyces promoters. This study thus greatly expands promoter diversity for the engineering of Streptomyces .
研究领域:
合成生物学
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分子遗传学
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产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
·
2019
·Vol. 121
·期 4
·1800431
·
IF 1.852
Jian-Yuan Yang,Jing Li,Mei Wang,Xian-Guo Zou,Bin Peng,Yu-Long Yin,Ze-Yuan Deng
Emulsion is inevitable during the aqueous extraction of oil from oilseeds. In this study, a new aqueous extraction method for camellia oil is investigated. During the extraction, the emulsified oil is collected, frozen, and thawed, and the free oil is obtained (AEEF). The optimal processing conditions are as follows: three times as much water as camellia seed powder (w/w), grinding at 70 °C for 6 min, repeating 2 cycles to collect the emulsified oil; then the emulsified oil is frozen (−20 °C) /thawed (50 °C) for 2 cycles. The yield of camellia oil is 89.37 ± 0.51%, which is significantly higher ( p < 0.05) than that of microwave-assisted aqueous enzymatic extraction (MAAEE, 83.05 ± 1.14%), and is similar to that of cold press extraction (CPE, 90.85 ± 2.05%), as well as solvent extraction (SE) coupled with refining. Additionally, the total oxidation value (TOTOX) of the free oil extracted by using AEEF (AEEF-Oil, 6.82 ± 0.12%) is significantly lower ( p < 0.05) than those of MAAEE, CEP, or SE. In comparison to SE-Oil and CEP-Oil, AEEF-Oil has higher ( p < 0.05) contents of α-tocopherol and squalene, and lower ( p < 0.05) benzo(a)pyrene contents, and contains more favorable natural volatile compounds. The results indicate that the novel AEEF may be a promising aqueous extraction method. Practical Application : The novel aqueous extraction method for camellia oil by emulsified oil – frozen/thawed has acceptable oil yields. The obtained camellia oil has high levels of bioactive components, low contents of hazardous substances, and more characteristic volatile flavor compounds. AEEF is an improved aqueous extraction technology for edible oil without refining and using n-hexane, which could be potentially applied in industrial production of camellia oil due to its safety, nutrition, and environment-friendly treatments. Aqueous extraction is a promising technique for edible oil extraction from oilseeds. The novel technology of aqueous extraction for camellia oil through emulsified oil – frozen/thawed (AEEF) is investigated. In comparison to solvent extraction, cold press extraction, and microwave-assisted aqueous enzymatic extraction of camellia oil, the obtained camellia oil by AEEF has an acceptable oil yield, high quality, and nutritional value. AEEF is a safe, simple and environmental-friendly aqueous extraction technology.
研究领域:
食品科学
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植物学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2022
·
IF 6.064
Luo Mingfang,Lv Yijie,Luo Xunrong,Ren Qingfa,Sun Zhenbo,Li Tianping,Wang Ailing,Liu Yan,Yang Caixia,Li Xianglin
With the development of nanotechnology, a theranostics nanoplatform can have broad applications in multimodal image-guided combination treatment in cancer precision medicine. To overcome the limitations of a single diagnostic imaging mode and a single chemotherapeutic approach, we intend to combat tumor growth and provide therapeutic interventions by integrating multimodal imaging capabilities and effective combination therapies on an advanced platform. So, we have constructed IO@MnO2@DOX (IMD) hybrid nanoparticles composed of superparamagnetic iron oxide (IO), manganese dioxide (MnO2), and doxorubicin (DOX). The nano-platform could achieve efficient T2-T1 magnetic resonance (MR) imaging, switchable photoacoustic (PA) imaging, and tumor microenvironment (TME)-responsive DOX release and achieve enhanced synergism of magnetic hyperthermia and chemotherapy with PA/MR bimodal imaging. The results show that IMD has excellent heating properties when exposed to an alternating magnetic field (AMF). Therefore, it can be used as an inducer for tumor synergism therapy with chemotherapy and hyperthermia. In the TME, the IMD nanoparticle was degraded, accompanied by DOX release. Moreover, in vivo experimental results show that the smart nanoparticles had excellent T2-T1 MR and PA imaging capabilities and an excellent synergistic effect of magnetic hyperthermia and chemotherapy. IMD nanoparticles could significantly inhibit tumor growth in tumor-bearing mice with negligible side effects. In conclusion, smart IMD nanoparticles have the potential for tumor diagnosis and growth inhibition as integrated diagnostic nanoprobes.
研究领域:
生物医学工程
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癌症治疗
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分子影像学
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产品关联
DOI
PubMed
高影响因子
Polymers
·
2022
·Vol. 14
·期 12
·2410
·
IF 4.967
Guoliang Ma,Zhiming Zheng,Han Wang,Li Wang,Genhai Zhao,Hengfang Tang,Xiumin Ding,Peng Wang
Nutritional food supplements and pharmaceutical products produced with vitamin K2as raw materials a very promising market in the global scope. The main production method of vitamin K2is microbial fermentation, but approximately 50% of vitamin K2synthesized by the main production strainBacillus subtilis nattoexists in extracellular form, which is not easy to separate and extract. In order to solve this problem, in this study, we synthesized a novel cellulose flocculant, MCC-g-LMA, by grafting reaction using microcrystalline cellulose (MCC) and lauryl methacrylate (LMA) as monomers, and ammonium persulfate as an initiator to flocculate VK2from the fermentation supernatant. The flocculant was characterized by Fourier transform infrared spectroscopy (FTIR), elemental analysis, and scanning electron microscopy (SEM), and the grafting reaction was successful. When the flocculant dosage was 48.0 mg/L and pH was 5.0, the flocculation rate of the MCC-g-LMA on the fermentation supernatant reached 85.3%, and the enrichment rate of VK2reached 90.0%. Furthermore, we explored the flocculation mechanism of VK2by the MCC-g-LMA and speculated that the flocculation mechanism mainly included adsorption bridging, hydrophobic association and net trapping and sweep effect. In this study, the extraction method for trace high-value biological products in the fermentation supernatant was improved, which provided a method and theoretical basis for the efficient separation and purification of VK2and other terpenoids.Keywords:cellulose;flocculant;Bacillus subtilis nattofermentation supernatant;vitamin K2
研究领域:
生物工程
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Oleo Science
·
2022
·
IF 1.628
Anning Liu,Huijuan Jing,Xiaojing Du,Chaoyang Ma,Hongxin Wang
In this study, the recovery of pecan nut kernel oil (PNKO) obtained by mechanical pressing (MP) was compared with that obtained by ultrasonic-assisted aqueous enzymatic extraction (UAAEE). At the same time, contents of substances with proven bioactivity, fatty acid compositions, physicochemical properties and antioxidant activities of PNKO were also assessed. Obviously, the oil yield obtained by UAAEE (71.32%) was higher than that obtained by MP (56.81%). Therefore, Plackett-Burman design (PBD), as well as Box-Behnken design (BBD), further optimized the UAAEE process, and the highest oil yield of 78.83% was acquired under the following conditions: incubation time of 2 h, the mixed enzyme (cell ulase+hemicellulase+pectinase+neutrase, w/w/w/w=1/1/1/1) concentration of 2.9%, liquid-solid ratio of 4 mL/g, pH of 4, particle size of 300 μm, ultrasonic time of 20 min, ultrasonic power of 432 W and reaction temperature of 53°C. The PNKO obtained by the two methods possessed similar fatty acid composition, but that obtained by UAAEE had comparatively low acid value and peroxide value. Moreover, rich content of total phenolics, squalene and phytosterols, as well as strong scavenging activities against DPPH and ABTS+ free radicals, were also contained in the PNKO obtained by UAAEE. These results demonstrated that UAAEE was an efficient method to acquire pecan nut kernel oil with excellent quality and high recovery.
研究领域:
食品科学
·
生物技术
·
化学工程
·
农业工程
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Qimeng Zhang,Qifan Zhao,Bingbing Zhu,Rong Chen,Yating Zhou,Xiaopeng Pei,Hua Zhou,Huiyong An,Ying Tan,Chengshui Chen
Pickering emulsions are attracting increased attention owing to their therapeutic applications. However, the slow-release property of Pickering emulsions and the in vivo solid particle accumulation caused by the solid particle stabilizer film limit their applications in therapeutic delivery. In this study, drug-loaded, acid-sensitive Pickering emulsions were prepared using acetal-modified starch-based nanoparticles as stabilizers. The acetalized starch-based nanoparticles (Ace-SNPs) not only act as a solid-particle emulsifier to stabilize Pickering emulsions but also exhibit acid sensitivity and degradability, conducive to the destabilization of Pickering emulsions to release the drug and reduce the effect of particle accumulation in an acidic therapeutic environment. In vitro drug release profiles show that 50 % of curcumin was released in 12 h in an acidic medium (pH 5.4), whereas only 14 % of curcumin was released in 12 h at higher pH (7.4), indicating that the Ace-SNP stabilized Pickering emulsion possess good acid-responsive release characteristics in acidic environments. Moreover, acetalized starch-based nanoparticles and their degradation products showed good biocompatibility, and the resulting curcumin-loaded Pickering emulsions exhibited significant anticancer activity . These features suggest that the acetalized starch-based nanoparticle-stabilized Pickering emulsion has the potential for application as an antitumor drug carrier to enhance therapeutic effects.
研究领域:
药学
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纳米技术
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药物递送系统
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癌症治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Synthetic and Systems Biotechnology
·
2023
·
IF 4.8
Zhongju Ji,Baolian Fan,Yidu Chen,Jingyang Yue,Jiabo Chen,Rongrong Zhang,Yi Tong,Zhongqiu Liu,Jincai Liang,Lixin Duan
Cibotium barometz (Linn.) J. Sm., a tree fern in the Dicksoniaceae family, is an economically important industrial exported plant in China and widely used in Traditional Chinese Medicine. C. barometz produces a range of bioactive triterpenes and their metabolites. However, the biosynthetic pathway of triterpenes in C. barometz remains unknown. To clarify the origin of diverse triterpenes in C. barometz , we conducted de novo transcriptome sequencing and analysis of C. barometz rhizomes and leaves to identify the candidate genes involved in C. barometz triterpene biosynthesis. Three C. barometz triterpene synthases (CbTSs) candidate genes were obtained. All of them were highly expressed in C. barometz rhizomes, consisting of the accumulation pattern of triterpenes in C. barometz . To characterize the function of these CbTSs, we constructed a squalene- and oxidosqualene-overproducing yeast chassis by overexpressing all the enzymes in the MVA pathway under the control of GAL-regulated promoter and disrupted the GAL80 gene in Saccharomyces cerevisiae simultaneously. Heterologous expressing CbTS1, CbTS2, and CbTS3 in engineering yeast strain produced cycloartenol, dammaradiene, and diploptene, respectively. Phylogenetic analysis revealed that CbTS1 belongs to oxidosqualene cyclase, while CbTS2 and CbTS3 belong to squalene cyclase. These results decipher enzymatic mechanisms underlying the origin of diverse triterpene in C. barometz .
研究领域:
植物生物化学
·
分子生物学
·
生药学
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产品关联
DOI
PubMed
高影响因子
POLYMER DEGRADATION AND STABILITY
·
2023
·
IF 5.9
Nouman Ali Shah,Rui Hong,Xue Yang,Shi-Shu Huang,Rizwan M. Gul,Yuwei Liu,Lingli Li,Jia-Zhuang Xu,Ka Li,Zhong-Ming Li
Maintaining reliability remains a great challenge for ultrahigh molecular weight polyethylene (UHMWPE) bearings due to oxidation in biological environments. This work aimed at inspecting the efficacy of the hybrid antioxidant strategy in stabilizing crosslinked UHMWPE under an in vitro clinically relevant accelerated aging condition. The hybrid antioxidant system was composed of vitamin E (VE) and D-sorbitol (DS) as primary and secondary antioxidants, respectively. The already established in vitro clinically relevant accelerated aging was performed via squalene absorption and subsequent thermal oxidation treatment. Compared to the use of VE alone, the combination of VE and DS significantly protracted the oxidation induction time of UHMWPE with no apparent oxidation after aging, showing a notable synergistic effect on the oxidative stability of UHMWPE. The crosslink density was higher for VE/DS/UHMWPE than VE/UHMWPE at each aging period. It revealed that the hybrid antioxidant strategy settled the longstanding contradiction between oxidation stability and crosslinking of UHMWPE. Attributed to improved oxidation stability, there was a positive reversal in the tensile property between VE/DS/UHMWPE and VE/UHMWPE after aging, where comparable ultimate tensile strength and higher elongation at break were observed. These findings underline the high potential of the hybrid antioxidant strategy for developing artificial joint materials with desired ability to resist oxidation and balanced performance.
研究领域:
生物医学工程
·
材料科学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2023
·
IF 5.2
Feng Wang,Chao Li,Haohao Wang,Lu Yu,Fuming Zhang,Robert J. Linhardt
Natural amino acid polymers have been wide-used as bio-compatible emulsion stabilizers . In this study, Amphiphilic O(Phe- r -Glu)s were synthesized through the papain-catalyzed random polymerization of L -phenylalanine and L -glutamate with the major degree of polymerization value of 6–7. The polymerization yield was increased by more than 35 % using choline to reduce the energy barrier for the amino substrates to form the tetrahedral intermediate with the catalytic triad. The squalene/water emulsions stabilized by O(Phe- r -Glu) nanoparticles were pH-insensitive at pH 1–12. The investigation of mechanism found the combined effects of steric hindrance , electrostatic repulsion and the viscosity of the emulsion contributed to the stability of the emulsion droplets. Under acidic condition, the layers of O(Pho- r -Glu)s particle aggregates formed around the emulsion droplets and the higher viscosity of emulsion mainly contributed to the stability of emulsions . At basic condition, the strong electrostatic repulsion between emulsion droplets covered by the negatively charged O(Pho- r -Glu) particles and the steric hindrance contributed mainly to the prevention of the coalescence of emulsion droplets. The application of enzymatically synthesized amphiphilic peptides in emulsion, along with tunable performance through the polymerization of amino substrates, is in line with the sustainable development requirements in fields such as pharmaceuticals, food and cosmetics.
研究领域:
生物材料学
·
食品科学
·
制药工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）