Small
·
2021
·Vol. 17
·期 40
·2102932
·
IF 13.281
Longyu Wang,Wenjun Qin,Wenxuan Xu,Fan Huang,Xiaochen Xie,Fei Wang,Lixin Ma,Cheng Zhang
By leveraging the ability of bacteria to express therapeutic protein cytolysin A (ClyA) through plasmid transformation, a thermally-activated biohybrid (TAB@Au) is constructed by biomineralizing gold nanoparticles (AuNPs) on the E. coli surface. Due to the feature of anaerobic bacteria homing to tumor microenvironments, the bacteria-based antitumor vehicles can be efficaciously accumulated at tumor sites. Under NIR laser irradiation, the biomineralized AuNPs harvest transdermal photons and convert them into local heat for photothermal therapy. After that, the produced heat elicits the expression of ClyA for killing tumor cells. In vitro and in vivo experiments verify the conception that the current therapeutic modality greatly inhibits the proliferation of tumor cells. In terms of the spatial specificity and non-invasiveness of NIR laser, the bacteria-based phototherapy represents an appealing way for tumor therapy.
研究领域:
生物医学工程
·
癌症研究
·
微生物学
·
诊疗学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2020
·
IF 4.389
Meilin Shi,Fengmei Zuo,Yingkai Tao,Yawen Liu,Jiahui Lu,Shaohui Zheng,Jiao Lu,Pingfu Hou,Jingjing Li,Kai Xu
Breast cancer is a refractory malignancy particularly in women, with an accruing morbidity and mortality worldwide. The purpose of this study was to evaluate the efficacy of laser-induced near-infrared (NIR) PFP/Gd-DTPA/ICG@PLGA NPs (PGINPs) in US/MR imaging and regimen for breast cancer; gadolinium-DTPA (Gd-DTPA), perfluoropentane (PFP) and indocyanine green (ICG) were wrapped in the poly (lactic-co-glycolic) acid (PLGA) shell membrane via a double emulsion approach. In this study, under the irradiation of NIR laser, the ICG enriched in the cancerous tissue not only converted optic energy into thermal energy to rapidly heat up the cancer focus but also convert O 2 to singlet oxygen ( 1 O 2 ), which can effectively destroy the cancer tissues through photothermal therapy (PTT) and photodynamic therapy (PDT). Meanwhile, the thermal energy thus generated could promote the gasification of PFP to enable visualization of cancer tissues under US imaging. Gd-DTPA in combination with US imaging can also significantly enhance MR imaging to provide US/MR dual-modal imaging. This study proves the efficacy of NIR-inspired multifunctional nano-system PGINPs to potentiate US/MR imaging and regimen for breast cancer.
研究领域:
肿瘤学
·
医学影像学
·
纳米医学
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2020
·
IF 4.389
Liang Hong,Jia-Lin Wang,Jun-Xian Geng,Yi-Hua Zhao,Gao-Xin Zhou,Jia Zhang,Li-Wei Liu,Jun-Le Qu
Photodynamic therapy (PDT) has emerged as one of the most promising modalities to treat cancers. However, the hypoxic microenvironment in tumors severely limits the efficiency of PDT. IR780 is a near-infrared light activatable photosensitizer for PDT. It has attracted intensive attention owing to its intriguing properties such as mitochondria-targeting ability and fluorescence imaging capability. Nevertheless, its application in tumor treatment is hampered by its low aqueous solubility and poor stability. To address these obstacles, here we designed a novel hierarchical nanoplatform containing a uniquely stable high loading capacity oxygen carrier (perfluoropolyether, in short, PFPE) and IR780. This nanoplatform (IR780-P/W NE, in abbreviation for IR780-PFPE-in-water nanoemulsion) has no detectable dark cytotoxicity. It not only improves the aqueous solubility and stability of IR780, but also transports oxygen to relieve hypoxia and boosts the efficiency of near-infrared light triggered PDT via augmentation of reactive oxygen species generation. Particularly, the innovative nanosized oxygen carrier developed in this research, P/W NE, is a potential universal platform for loading hydrophobic photosensitizers (including but not limited to IR780), sonosensitizers, or radiosensitizers, and simultaneously improving the therapeutic efficacy. Our results highlight the intriguing potential of the developed nanoemulsions for mitigating tumor hypoxia and enhancing the efficiencies of oxygen-dependent therapies including PDT, sonodynamic therapy, radiotherapy, and so on.
研究领域:
癌症治疗
·
纳米医学
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2016
·
IF 3.902
Shenna Chen,Haowen Huang,Lingyang Zhang,Yun Chen,Xuanyong Liu
In this study, a strategy for load and release of different kinds of molecules on the same gold nanorods (GNRs) was developed. An anticancer drug, doxorubicin hydrochloride (DOX), was firstly chemically conjugated on the GNRs. To efficiently load another type of target molecules DNA on the same GNRs, a polyelectrolyte Poly (ethylene imine) (PEI) was adsorbed on the [email protected] to form [email protected] @PEI. Then, the positive charge [email protected] @PEI allows the GNR conjugates to interact with negative charged DNA by an electrostatic interaction, enabling their full conjugation. A platform to load two kinds of target molecules conjugated on the same GNRs was fabricated. On the other hand, selective and sequential release of the different target species may be triggered by chemical reaction and near infrared (NIR) laser. The release of DOX was achieved by Na 2 S 2 O 3 reacting with GNRs and the discharge of DNA conjugated on the [email protected] @PEI was accomplished by local-heating using NIR laser triggered release. Furthermore, the selective and alternate release of different target species from the GNRs inside MCF-7 cells was monitored by fluorescent imaging, providing a potential synergistic cancer treatment.
研究领域:
纳米技术
·
癌症研究
·
生物医学工程
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2025
·
IF 4.7
Wenjie Ma,Ru Jia,Lanlan Dong,Cijun Shuai,Wurikaixi Aiyiti,Chongxian He
Transplant-associated bacterial infections, especially the formation of bacterial biofilms, frequently lead to persistent infections and even transplant failure. Herein, a near-infrared laser-triggered nanosystem with antibacterial and anti-biofilm activities is constructed by loading S-nitrosothiols (RSNO) onto Ti 3 C 2 T x nanosheets, which are then mixed with poly-L-lactic acid (PLLA) powders to prepare porous PLLA/Ti 3 C 2 T x -SNO scaffolds. Especially, the excellent photothermal property of the scaffold is expected not only to eliminate bacterial biofilms but also to trigger RSNO molecules to in situ release nitric oxide (NO) gas under near-infrared laser irradiation. Moreover, the removal of the biofilms facilitates more NO to enter and kill the deep bacteria through nitridation damage, thus realizing the synergistic antibacterial capabilities of photothermal and NO gas therapies. The experimental results showed that the photothermal conversion efficiency of the scaffold reached 39 %. Combining with the in situ NO release, the scaffold presented excellent anti-biofilm and antibacterial activities, with antibacterial rates of 93.6 % and 94.9 % against E. coli and S. aureus , respectively. This work is expected to solve the dilemma of treating bacterial infections in deep tissues, providing reference for the clinical treatment of transplant-associated bacterial infections and even extending to the treatment of other deep refractory infections.
研究领域:
纳米技术
·
抗菌治疗
·
光热治疗
·
生物材料
·
传染病
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Xinghui Yu,Wenyang Shi,Junyan Hou,Kairui Zhai,Yang Xu,Jie Zhao,Xiaodong Lin
Escherichia coli ( E. coli ) is a leading cause of hospital-acquired infections, especially in abdominal and urinary tract sites, posing serious risks for patients’ post-kidney transplantation. Rapid and precise detection of E. coli is critical to prevent complications such as bloodstream infections, urinary tract infections, and infectious shock. Here, we present a prolong glow-type chemiluminescence (CL)-based bacterial metabolism platform that achieves both sensitive detection and effective photothermal inactivation of E. coli based on multifunctional nanomaterials. This platform leverages the metabolic activity of E. coli to trigger a CL signal without the need for external excitation sources, facilitating low-cost and highly sensitive detection with a limit of detection as low as 1 CFU/mL. Fe 3 O 4 NPs were functionalized with E. coil -specific antibodies, allowing for precise pathogen capture and magnetic separation, ensuring robust sample preparation. After detection, near-infrared (NIR) laser irradiation enabled the Cu 2-X Se NPs to generate localized heat, effectively inactivating E. coli cells with an antibacterial efficiency of up to 99.18%. This multifunctional platform integrates rapid detection and sterilization, demonstrating significant potential for clinical applications in infection management, especially in post-transplant care.
研究领域:
微生物学
·
感染病学
·
纳米医学
·
临床检验
·
移植医学
·
分子诊断
·
生物传感
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 8
·1260-1271
·
IF 4.776
Qian Zhao,Yunhong Liang,Lei Ren,Zhenglei Yu,Zhihui Zhang,Feng Qiu,Luquan Ren
A novel type of nanofibrillated cellulose (NFC)-containing poly N-isopropylacrylamide-clay bilayer nanocomposite hydrogel actuator was successfully fabricated via one-step in situ free radical polymerization. The bilayer hydrogel exhibited rapid, reversible and efficient temperature and near infrared laser responses in air and water. With an increase in the NFC content, the cross-density of hydrogels enhanced, leading to an increase in the strength and deswelling rate and a decrease in the strain, swelling rate and bending degree. The two layers in the bilayer structure bonded tightly. The bilayer hydrogel dimensions affected the deformation ability. The smaller the bilayer hydrogel, the higher the bending degree exhibited by the actuators. Bending degree in poikilothermy temperature ranging from 32 to 50 °C was higher than that in the constant temperature of 50 °C. Based on the intelligent responsive property, the bilayer hydrogel was designed as a soft temperature-controlled manipulator, which can be treated as a candidate material for medicine and soft robotics.
研究领域:
生物医学工程
·
智能材料
·
软体机器人
·
药物递送系统
·
响应性水凝胶
✓ 已验证
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2022
·Vol. 57
·期 7
·4754-4770
·
IF 4.682
Zhuang Yi,Kan Zhaojun,Jiang Yulin,He Miaomiao,Zhang Yuanyuan,Sun Xiaodong,Du Meixuan,Wang Jing,Li Yubao,Li Yunfeng,Zhang Li
In this study, we prepared a drug-laden graphene-incorporated calcium alginate (GCA) microspheres, which were then blended with the typically thermosensitive hydrogel named poly (d,l-lactide-co-glycolide)-poly (ethylene glycol)-poly (d,l-lactide-co-glycolide) triblock copolymer (PLGA-PEG-PLGA, PPP for short) to obtain an on-demand drug controlled release system triggered by near-infrared (NIR) laser, thus achieving minimally invasive treatment of tumorous defect through the in-situ curing of the GCA/PPP hydrogel after injection into the target site. The results indicate that all these drug-laden GCA/PPP hydrogels are injectable, enabling the minimally invasive treatment of defects with complex geometries and shapes. The 2%GCA/PPP could be heated up to 45.3 ºC within 3 min under NIR irradiation and showed a unique heating plateau in the heating curve, performing the desired photothermal stability and heating-up effect in comparison with the other groups. By turning on or off the NIR irradiation, GCA/PPP hydrogels could exhibit the step-shaped drug release curves, meaning that on-demand controlling the drug release is feasible by using NIR laser as the trigger. The in vitro biological assessments showed that the drug-laden GCA/PPP hydrogels were biocompatible as well as enhanced anti-tumor capability under NIR exposure, demonstrating their promise in treatment of tumorous defects with irregular shapes.
研究领域:
生物医学工程
·
材料科学
·
肿瘤学
✓ 已验证
产品关联
DOI
高影响因子
Nano Energy
·
2018
·
IF 13.12
Qian Zhao,Yunhong Liang,Lei Ren,Zhenglei Yu,Zhihui Zhang,Luquan Ren
Inspired by common patterns of biological movement, a series of near-infrared (NIR) laser-driven bionic intelligent hydrogel actuators were successfully fabricated via the combination of infiltration method (entirety infiltration and locality infiltration), structure design and material characteristics. The controllable distribution of graphene oxide (GO) particles in a hydrogel matrix resulting from the infiltration method led to abundant bionic deformations with multiple degrees of freedom and flexibility. In addition to the highest response speed of 187.7°/s, the hydrogel actuators underwent accurate and repeatable static deformations and efficient and stabilized dynamic locomotion, demonstrated, for example, through the assembly of origami, the deformation of fingers and palm, the closing and blooming of a chrysanthemum and the crawling of an inchworm. This study opens a new path for practical bionic applications of NIR-triggered hydrogel actuators in self-assembly biosensor, targeted drug delivery and minimally invasive surgery.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale
·
2015
·Vol. 7
·期 17
·7682-7691
·
IF 7.394
Zhiyou Peng,Jinbao Qin,Bo Li,Kaichuang Ye,Yuxin Zhang,Xinrui Yang,Fukang Yuan,Lijia Huang,Junqing Hu,Xinwu Lu
Photothermal therapy (PTT), as a promising treatment for tumours, has rarely been reported for application in artery restenosis, which is a common complication of endovascular management due to enduring chronic inflammation and abnormal cell proliferation. In our study, biodegradable polypyrrole nanoparticles (PPy-NPs) were synthesized and characterized, including their size distribution, UV-vis-NIR absorbance, molar extinction coefficients, and photothermal properties. We then verified that PPy-NP incubation followed by 915 nm near-infrared (NIR) laser irradiation could effectively ablate inflammatory macrophages in vitro, leading to significant cell apoptosis and cell death. Further, it was found that a combination of local PPy-NP injection with 915 nm NIR laser irradiation could significantly alleviate arterial inflammation by eliminating infiltrating macrophages and further ameliorating artery stenosis in an ApoE−/− mouse model, without showing any obvious toxic side effects. Thus, we propose that PTT based on PPy-NPs as photothermal agents and a 915 nm NIR laser as a power source can serve as a new effective treatment for reducing inflammation and stenosis formation in inflamed arteries after endovascular management.
研究领域:
生物医学工程
·
心血管疾病
·
纳米技术
·
治疗方法
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Biomaterials Science & Engineering
·
2019
·
IF 4.511
Qiwen Li,Junru Wen,Chenlu Liu,Yanpeng Jia,Yongzhi Wu,Yue Shan,Zhiyong Qian,Jinfeng Liao
Hydrogel is an ideal scaffold in the fields of regenerative medicine and tumor therapy because of its biomimetic ability to modulate tissue microenvironment. Herein, we fabricated a new kind of self-healing hydrogel based on graphene nanoparticle and expanded its application in postoperative recurrence of breast cancer. First, a facile method was used to prepare self-healing hydrogel via Schiff-base linkage, which composed of chondroitin sulfate multialdehyde (CSMA), branched polyethylenimine (BPEI) and BPEI conjugated graphene (BPEI-GO). BPEI-GO was doped in the network and participated in Schiff-base reaction and stabilized the structure, as well as provided sustained drug delivery, and near-infrared laser (NIR)-triggered photothermal effect. The hydrogels exhibited excellent self-healing (∼100%) and improved mechanical properties (7,000 Pa). Further, in vitro breast cancer cell inhibition study showed enhanced cell killing efficiency with synergistic chemo-photothermal therapy. In the breast cancer postoperative recurrence prevention mice model, we found that combination of Doxorubicin (DOX) and photothermal therapy in CSMA/BPEI/BPEI-GO hydrogels group reduced tumor recurrence to 33.3%, compared with 66.7% for DOX-loaded hydrogels without NIR irradiation, 66.7% for local administration of free DOX, 100% for hydrogels with NIR irradiation, blank hydrogels, and blank control. This study suggests the great potential of CSMA/BPEI/BPEI-GO hydrogels for postoperative recurrence prevention of breast cancer.
研究领域:
生物医学工程
·
肿瘤学
·
再生医学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Guolin Zheng,Zengdi Zhao,Aming Wang,Haonan Cheng,Xinjun Bao,Hengshu Zhou,Dan Zhang,Qiang Wang
Inspired by lotus leaf, biomimetic hydrophobic cellulose fiber-based textiles were usually fabricated by the lower surface energy materials. However, it is rare to witness a hydrophobic surface obtained by near infrared laser (NIR) irradiation a superhydrophilic nanomaterial, which remains a huge challenge. Herein, the CuS nanospheres was in-situ anchored on the surface of gallic acid self-polymer treated cotton fabric. Luckily, the excellent hydrophobicity of CuS nanospheres coating (contact angles of 142.7 ± 2.8°) was for the first time demonstrated after a NIR laser irradiation with 1.5 W/cm 2 for 8–10 s. Notably, the novel hydrophobicity exhibits a significant advance compared with traditional hydrophobicity prepared by lower surface energy materials. Then, the laser-mediated hydrophobic mechanism was demonstrated by the cold-field emission scanning electron microscope (FESEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and thermal imager. Moreover, the dynamic contact angle revealed the uniform hydrophobicity. Furthermore, the hydrophobic nanospheres coating possessed good photothermal effect and UV resistance. Overall, this work renders a novel, feasible, and efficient strategy to rapidly obtain the hydrophobic surface by NIR laser-induced the superhydrophilic CuS nanospheres coating.
研究领域:
材料科学
·
表面化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）