RSC Advances
·
2023
·Vol. 13
·期 8
·5149-5157
·
IF 3.9
Xiaoxiao Wu,Xushuo Yuan,Jiaxin Zhao,Decai Ji,Haiyang Guo,Wentao Yao,Xiaoping Li,Lianpeng Zhang
With the development of science and technology, efficient, fast and green methods are increasingly being pursued. The production of nanocellulose by green methods, such as bio-enzymes-assisted ultrasound treatment, has been the focus of many studies. However, the yield of cellulose nanocrystals prepared by this method is very low. In this paper, by pretreatment of microcrystalline cellulose (MCC), nanocellulose was prepared by heating and stirring + pectinase/cellulase + ultrasonic treatment (HSt – P/C – Ultr). The effects of the ratios of pectinase and cellulase and the hydrolysis time on the yield of nanocellulose were studied. FTIR, XRD, SEM, TEM and TG were used to determine the structure, crystallinity, morphology and thermal stability of nanocellulose. The results showed that optimal hydrolysis conditions were determined as a pectinase : cellulase ratio of 1 : 1, 90 min and 50 °C. The yield of nanocellulose was about 32.0%. The yield of pectinase cellulase = 1 : 1 was higher than that of microcrystalline cellulose (MCC) treated by a single bio-enzyme. This indicated that the synergistic effects of pectinase and cellulase have a certain effect on the formation of nanocellulose. During the preparation, the crystalline form of cellulose did not change. It was still cellulose I with a crystallinity of 73.5%, which is 9.50% higher than that of microcrystalline cellulose (MCC), a width of 20–50 nm, a high aspect ratio and a winding network structure. Therefore, nanocellulose prepared by this method is an ideal toughening material for manufacturing composite materials.
研究领域:
材料科学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL SCIENCES
·
2019
·
IF 1.618
Ruru Chen,Lian Zhou,Hui Yang,Hailing Zheng,Yang Zhou,Zhiwen Hu,Bing Wang
The degradation behavior of silk fibroin (SF) is a significant and intriguing subject in the area of archaeological ancient silk research. In the present study, the immunological detection techniques combined with traditional characterization methods, jointly studied the degradation process of SF from Bombyx mori (B. mori) and Antheraea pernyi (A. pernyi) through exposure to alkaline proteinase, α-chymotrypsin, pepsin, and trypsin. Spectroscopic analysis revealed that different enzymes showed similar hydrolysis effects on the secondary structure, but the changes of B. mori SF and A. pernyi SF were mainly reflected in the decrease of β-sheet and the reduction of α-helical structure, respectively. In further research of immunology, two diagnostic antibodies were prepared corresponding to SF of B. mori and A. pernyi, respectively. The enzyme-linked immunosorbent assay (ELISA) and western blot indicated the enzyme-treated SF proteins still exhibited higher immunoreactivity because the epitopes on the surface of SF molecules are retained. Although α-chymotrypsin possesses the most cleavage sites among these enzymes, the α-chymotrypsin-treated SF did not exhibit significant changes in secondary structures and high antibody binding capacity. The results deepen our understanding of the SF degradation process during enzymatic hydrolysis, and show far-reaching guiding significance in trace detection of SF.
研究领域:
考古学
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材料科学
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生物化学
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免疫学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2019
·Vol. 43
·期 11
·e13027
·
IF 1.358
Mengnan Hong,Jing Li,Yuwen Chen
This work aim to study the tolerance and aroma-related enzymes activities of the non- Saccharomyces yeasts in Vidal blanc icewine from the Huanren region of China. The strains were identified by sequencing internal transcribed spacer (ITS) region and the 26S rDNA D1/D2 domain genes and all representative non- Saccharomyces yeasts were belonged to genera Metschnikowia , Hanseniaspora , Torulaspora, Candida , and Debaryomyces . A total of 28 strains were carried out for tolerance experiments and results suggested that most of them could tolerate 500 g/L glucose, 4% ethanol, 20 g/L tartaric acid, and 350 mg/L SO 2 . Finally, a total of 17 strains with better tolerance were carried out for the β-glucosidase, β-xylosidase, and pectinase activities experiments. The results showed that Candida railenensis HC08 and the strains of Hanseniaspora genus have satisfactory multi-enzyme activities, which can be used to design mixed fermentation to produce characteristic icewine. Practical applications Non- Saccharomyces yeasts produces a series of hydrolytic enzymes that are thought to have a significant contribution to the aroma complexity of wines, however, are poorly explored in icewine. In this work, most of the non- Saccharomyces yeasts screened from Chinese icewine can adapt well to the high-sugar and high-acid environment of icewine, and can secrete hydrolase. The application of these strains in mixed fermentation could provide a prospect for the production of characteristic icewine.
研究领域:
酿酒学
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微生物学
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食品科学
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产品关联
DOI
PubMed
高影响因子
SOLID STATE SCIENCES
·
2024
·
IF 3.4
Ying Zhu,Tongkai Ji,Xingwei Cai,Jing Wang
A novel NiO/CeO 2 −mSiO 2 nanocages with superior peroxidase−like activities due to the synergetic effect between NiO and CeO 2 were fabricated by a simple etching−precipitation method with core−shell structured Cu 2 O@mSiO 2 as a sacrificial template. Based on the peroxidase−like activity of the NiO/CeO 2 −mSiO 2 nanocages, an enzyme cascade−triggered colorimetric sensing platform was exploited for alkaline phosphatase (ALP) detection, owing to the fact that ascorbic acid (AA) that is the ALP enzymatic hydrolysis product of ascorbic acid 2−phosphate (AAP) can efficiently reduce the oxidation product of TMB (oxTMB) generated under the catalysis of the NiO/CeO 2 −mSiO 2 . For the sensing of ALP, a good linear response was observed in the range of 0.0005–1.25 mU mL −1 with the limit of detection (LOD) as low as 0.00048 mU mL −1 . Furthermore, the proposed enzyme cascade−triggered colorimetric sensing platform could be further explored for sensing of tyrosinase, according to the fact that dopamine (DA) the catalytic product of tyramine (Tyr) by tyrosinase could initiate the redox reaction with the oxTMB acquired under the catalysis of the NiO/CeO 2 −mSiO 2 . A wide linear response was found for tyrosinase detection from 0.02 mU mL −1 to 3.4 U mL −1 with a LOD as low as 0.002 U mL −1 . This work not only offers a facile and effective approach to fabricate nanozymes with excellent catalytic activity, but also provides a promising enzyme cascade−triggered colorimetric method for the ultra−sensitive detection of biological enzymes.
研究领域:
生物化学
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纳米医学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
NATURE
·
2025
·
IF 48.5
Zhu Bao,Zhang Yanjun,Gao Rong,Wu Zhihua,Zhang Wei,Zhang Chao,Zhang Penghong,Ye Can,Yao Linbo,Jin Ying,Mao Hui,Tou Peiyao,Huang Peng,Zhao Jiangzhe,Zhao Qiao,Liu Chang-Jun,Zhang Kewei
Salicylic acid (SA) is a pivotal phytohormone for plant responses to biotic and abiotic stresses. Plants have evolved two pathways to produce SA: the isochorismate synthase and phenylalanine ammonia lyase (PAL) pathways 1 . Whereas the isochorismate synthase pathway has been fully identified 2 , 3 , 4 , the PAL pathway remains incomplete. Here we report the full characterization of the PAL pathway for SA biosynthesis via functional analysis of rice ( Oryza sativa ) SA-DEFICIENT GENE 1 ( OSD1 ) to OSD4 . The cinnamoyl-coenzyme A (CoA) ligase OSD1 catalyses the conversion of trans -cinnamic acid to cinnamoyl-CoA, which is subsequently transformed to benzoyl-CoA via the β-oxidative pathway in peroxisomes. The resulting benzoyl-CoA is further converted to benzyl benzoate by the peroxisomal benzoyltransferase OSD2. Benzyl benzoate is subsequently hydroxylated to benzyl salicylate by the endoplasmic reticulum membrane-resident cytochrome P450 OSD3, which is ultimately hydrolysed to salicylic acid by the cytoplasmic carboxylesterase OSD4. Evolutionary analyses reveal that the PAL pathway was first assembled before the divergence of gymnosperms and has been conserved in most seed plants. Activation of the PAL pathway in rice significantly enhances salicylic acid levels and plant immunity. Completion of the PAL pathway provides critical insights into the primary salicylic acid biosynthetic pathway across plant species and offers a precise target for modulating crop immunity.
研究领域:
植物学
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生物化学
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分子生物学
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遗传学
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农学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Nan Qiu,Qi-Yan Lv,Chun-Ling Li,Xiaojie Song,Yu-Qian Wang,Junyang Chen,Hui-Fang Cui
Immobilization of proteolytic enzymes onto nanocarriers is effective to improve drug diffusion in tumors through degrading the dense extracellular matrix (ECM). Herein, immobilization and release behaviors of hyaluronidase, bromelain, and collagenase (Coll) on mesoporous silica nanoparticles (MSNs) were explored. A series of cationic MSNs (CMSNs) with large and adjustable pore sizes were synthesized, and investigated together with two anionic MSNs of different pore sizes. CMSNs4.0 exhibited the highest enzyme loading capacity for hyaluronidase and bromelain, and CMSNs4.5 was the best for Coll. High electrostatic interaction, matched pore size, and large pore volume and surface area favor the immobilization. Changes of the enzyme conformations and surface charges with pH, existence of a space around the immobilized enzymes, and the depth of the pore structures, affect the release ratio and tunability. The optimal CMSNs-enzyme complexes exhibited deep and homogeneous penetration into pancreatic tumors, a tumor model with the densest ECM, with CMSNs4.5-Coll as the best. Upon loading with doxorubicin (DOX), the CMSNs-enzyme complexes induced high anti-tumor efficiencies. Conceivably, the DOX/CMSNs4.5-NH 2 -Coll nanodrug exhibited the most effective tumor therapy, with a tumor growth inhibition ratio of 86.1 %. The study provides excellent nanocarrier-enzyme complexes, and offers instructive theories for enhanced tumor penetration and therapy.
研究领域:
药学
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纳米技术
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癌症研究
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药物递送系统
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产品关联
DOI
PubMed
高影响因子
CHEMISTRY AND ECOLOGY
·
2017
·
IF 1.463
Haixia Zhan,Jinping Zhang,Zhu Chen,Yongjie Huang,Teija Ruuhola,Shiyong Yang
Cadmium (Cd) is a toxic non-essential element accumulated easily in living organisms due to its non-biodegradable nature. A slight increase in Cd levels will threaten the health of terrestrial and aquatic organisms. Therefore, Cd contamination and its effects on the ecosystems and organisms have become a major topic of eco-toxicological research. The present study investigated the eco-toxicological effects of Cd-supplemented diet on some life history traits and specific enzyme activities of carboxylesterase (CarE), glutathione S-transferase (GST), acetylcholinesterase (AChE) and cytochrome P450 monooxygenases (P450s) in the cotton bollworm, Helicoverpa armigera. The results showed that Cd significantly decreased larval survival, pupation and emergence, and reduced body mass of the sixth instar larvae, pupae, adult moths and female fecundity. Cd-supplemented diet also delayed the development of larvae and pupae and shortened the life span of the cotton bollworm moth. In addition, dietary Cd inhibited the activities of GST, CarE and P450, but increased AChE activity of the sixth instar larvae. We concluded that life history traits and metabolic enzymes are sensitive to Cd stress in the cotton bollworm. These parameters are potential biomarkers for monitoring environmental stress in Lepidopteran insect pests.
研究领域:
生态毒理学
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昆虫学
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环境科学
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产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2022
·
IF 6.14
Haiyan Ruan,Shaofang Zhang,Hongguang Wang,Jiahui Pei,Ruoli Zhao,Xiaoyu Mu,Hao Wang,Xiaodong Zhang
The construction of nanozymes at the atomic level that hold structural stability and high enzyme-like activity is now a key factor in the optimization of an artificial enzyme. Single-atom metal/cerium oxide (CeO2)-based nanozymes have been demonstrated to possess a variety of enzymatic activities and radical scavenging abilities, which are mainly attributed to the single-atom active site, redox valence states, and abundant defect chemistry. Here, we developed a single-atom Pd/CeO2 nanostructure by aqueous phase synthesis that exhibits the advantages of high yield and good stability. The Pd/CeO2 nanostructure possesses peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) activities as well as reactive nitrogen species free-radical scavenging activity, exhibiting multienzyme-like activities and stability compared with CeO2 and other metal-based nanozymes. It is worth mentioning that the Pd/CeO2 nanostructure exhibits high POD-mimicking activity with a reaction rate of 0.88 μM/min, about 5 times higher than that of the CeO2 nanozyme. In addition, the CAT-like activity of the Pd/CeO2 nanostructure is excellent, and its scavenging rate of hydrogen peroxide reached nearly 100% at a concentration of 50 ng/μL. The present work shows that single-atom Pd substitution is a promising strategy for the design of CeO2 nanozymes to exert better effects on biomedical applications, especially with diseases related to oxidative stress.
研究领域:
纳米技术
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生物化学
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材料科学
·
生物医学工程
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Shiming Tang,Zhilin Ouyang,Ying Huo,Daocheng Liao,Zhiteng Zhang,Ying Lin,Suiping Zheng
Hydroxytyrosol has been proven beneficial to human health. However, the process involving the conversion of L-DOPA to 3,4-dihydroxyphenylacetaldehyde (3,4-DHPAA) in hydroxytyrosol biosynthesis typically required the simultaneous use of decarboxylase and oxidative deaminase. In addition, phenylacetaldehyde reductase from Solanum lycopersicum (SlPAR) in hydroxytyrosol biosynthesis exhibits poor thermal stability. In this study, we unexpectedly discovered that L-DOPA decarboxylase from Pseudomonas putida (PpDODC) exhibits weak dual-functional activity for both decarboxylation and oxidative deamination of L-DOPA. Through a dual-function reshaping strategy, the best dual-functional mutant PpDODC/Y79F/Y324F achieved an enzyme activity of 0.95 U/mg and exhibited a 256.8-fold increase in activity compared to the wild-type. Through rational design of SlPAR, the optimal mutant SlPAR/G52D/V188I/N234W/Q286P (SlPAR-M4) maintained stability after 12 h' treatment at 40 °C. Based on these mutants, we established a simplified cascade to synthesize hydroxytyrosol from L-DOPA, achieving a hydroxytyrosol yield of 31.4 mM from 32 mM L-DOPA in a 5-hour reaction. This process achieved the highest molar conversion rate (98.2 %) currently reported for the synthesis of hydroxytyrosol from L-DOPA. This study provides a novel solution for hydroxytyrosol synthesis from a new perspective, and the mutants hold promise for widespread application in biotransformation studies of hydroxytyrosol and other structurally-similar compounds.
研究领域:
生物化学
·
生物技术
·
酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2025
·Vol. 90
·期 5
·e70302
·
IF 3.4
Pengfei Zhou,Hui Fang,Zhihao Zhao,Guang Liu,Jiarui Zeng,Yuanyuan Deng,Mingwei Zhang
The precise regulation of enzyme conformation through the immobilization of enzymes on carriers using sewing techniques represents a key focus in the site-specific chemically modification engineering. Lipase-mediated enzymatic interesterification (EIE) is of considerable significance in lipid chemistry, having demonstrated substantial potential in the modification of lipids. In this study, we developed an innovative nanomaterial Fe 3 O 4 -SiO 2 -APTES for site-specific immobilization of Thermomyces lanuginosus lipase (TLL), achieving an immobilization yield of 51.98% and an enzyme loading capacity of 123.18 mg/g. The specific activity of the enzyme was 1034.09 U/g following lyophilization, representing increases of 2.6-fold compared to free TLL. The as-prepared biocatalyst TLL@Fe 3 O 4 -SiO 2 -APTES exhibited enhanced catalytic activity, remarkable tolerance to organic solvents, substrate selectivity, and recyclability over multiple cycles. Rice bran oils (RBO) and palm stearin (PS) were employed as substrates for EIE, yielding oils with better performance of solid fat content (SFC), crystallization rate, and thermodynamic properties compared to those of physical blending oils. Additionally, we investigated the catalytic mechanism of EIE using molecular docking analysis. The present study provides a theoretical foundation for the application of immobilized lipase as biocatalysts in food industrial settings. Practical Application : TLL@Fe 3 O 4 -SiO 2 -APTES, modified at specific sites using nanomaterials, not only enhances the catalytic efficiency of the enzyme but also facilitates its reuse across multiple batches. This fixed enzyme could effectively catalyze the transesterification between PS and RBO, resulting in high-quality of oils specifically designed for baking foods. The findings in this study provide valuable guidance for the green processing of oils and fats.
研究领域:
食品科学
·
生物化学
·
酶工程
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Zepeng Kang,Yi-Heng P. Job Zhang,Zhiguang Zhu
In this study, a novel carbon tube was prepared by carbonizing a rectangular polypyrrole (RPPy) tube at a high temperature for the construction of enzymatic biofuel cells with high performance. SEM and TEM images clearly showed that the initial PPy presented a rectangular tube shape, while the carbonized PPy became a shriveled rectangular tube with a concave surface, which might be beneficial for enzyme immobilization and electrochemical applications. The glucose oxidase (GOx)- or laccase (Lac)-modified electrodes based on carbonized RPPy exhibited excellent bioelectrochemical performance. In addition, a biofuel cell (GOx, glucose/O 2 , Lac) was assembled, and the open-circuit voltage reached 1.16 V. The maximum power density was measured to 0.350 mW cm −2 , which correlated to the gravimetric power density of 0.265 mW mg −1 (per mg of GOx) at 0.85 V. The constant-current discharge method was used to further evaluate the continuous discharge capacity. The discharge time reached 49.9 h at a discharge current of 0.2 mA before the voltage was lower than 0.8 V. Furthermore, three of the fabricated biofuel cells in series were able to continually light up a white light-emitting diode (LED) whose turn-on voltage was ca. 2.4 V for more than 48 h. This study suggests that carbonized conducting polymers may become a useful electrode material for the development of enzymatic biofuel cells.
研究领域:
生物化学
·
材料科学
·
能源科学
✓ 已验证
DOI
高影响因子
CHEMICAL PHYSICS LETTERS
·
2017
·
IF 1.815
W.S. Huo,H. Zeng,Y. Yang,Y.H. Zhang
Enzymatic electrodes over-coated by thin film of nano-composite made up of polymer and functionalized nano-gold particle was prepared. Glucose/O 2 membrane-free enzymatic fuel cell based on nano-composite based electrodes with incorporated glucose oxidase and laccase was assembled. This enzymatic fuel cell exhibited high energy out-put density even when applied in human serum. Catalytic cycle involved in enzymatic fuel cell was limited by oxidation of glucose occurred on bioanode resulting from impact of sophisticated interaction between active site in glucose oxidase and nano-gold particle on configuration of redox center of enzyme molecule which crippled catalytic efficiency of redox protein.
研究领域:
生物化学
·
纳米生物学
·
能源科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）