FOOD CHEMISTRY
·
2017
·
IF 4.529
Le-Le Shao,Jie Xu,Mei-Jia Shi,Xiao-Ling Wang,Yin-Ting Li,Li-Min Kong,Robert C Hider,Tao Zhou
In order to improve the antioxidant and antimicrobial abilities, hydroxamated degraded polysaccharides from Enteromorpha prolifera (HCDPE) were prepared from the corresponding carboxymethylated degraded polysaccharides (CDPE). HCDPE was characterized by FT-IR. The weight-average molecular weight of HCDPE was determined as 55.4 kDa. The in vitro antioxidant activity of HCDPE was evaluated by determining the radical (1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl and superoxide anion radicals) scavenging abilities and total antioxidant activity. It was found that DPPH radical scavenging ability and total antioxidant activity of HCDPE were significantly improved compared to those of CDPE. The inhibitory effects of polysaccharides against the five bacterial strains ( Bacillus subtilis , Staphylococcus aureus , Escherichia coli , Pseudomonas aeruginosa , Salmonella spp.) were also evaluated by bacterial inhibition zone and minimum inhibitory concentration (MIC) assays. The results indicated that CDPE and HCDPE possess marked antimicrobial ability, while such an effect was not observed for the crude polysaccharides (PE) and the degraded polysaccharides (DPE).
研究领域:
海洋生物学
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食品科学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WATER RESEARCH
·
2020
·
IF 9.13
Shu Wang,Bingliang Zhang,Chao Shan,Xing Yan,Hong Chen,Bingcai Pan
Phosphonates discharged from wastewater treatment plants (WWTPs) have attracted increasing concerns because of their potential impact on eutrophication and potential risks to aquatic ecosystems. However, very few studies are available on their occurrence and transformation in WWTPs, partly due to the lack of sensitive methods for phosphonate analysis in complex matrices. Herein, based on our recent progress in phosphonate analysis, the occurrence and transformation of phosphonates along the full-scale wastewater treatment processes of two textile dyeing WWTPs were revealed. A set of typical phosphonates, including six phosphonate chelators (PCs) and four potential degradation products of PCs (DP-PCs) were quantified in different units and the final dewatered sludge. Three PCs (2-phosphonobutane-1,2,4-tricarboxylic acid (PBTC), 1-hydroxyethane 1,1-diphosphonic acid (HEDP) and nitrilotris(methylene phosphonic acid) (NTMP)) at upmost mg/L and a considerable amount of four DP-PCs (9.12–608 μg/L) were detected in the influents of both WWTPs. In the subsequent treatment, NTMP could be removed more efficiently than PBTC and HEDP, especially in the coagulation unit, and the dissolved phosphonates were eliminated more readily than other dissolved organic phosphorus fractions. Of particular note, the toxicologically critical DP-PC (i.e., aminomethylphosphonic acid) was produced during the coagulation and biological treatment units. The final precipitation unit seemed essential to ensure satisfactory removal of PCs and DP-PCs. In addition, a significant accumulation of phosphonates in dewatered sludge (up to 7.81 g/kg) and the widespread occurrence of harmful DP-PCs also reminded us to pay more concerns on their potential risks during further sludge disposal in future.
研究领域:
环境科学
·
水处理工程
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DOI
高影响因子
CHROMATOGRAPHIA
·
2019
·Vol. 83
·期 1
·87-93
·
IF 1.552
Cui Peng,Fang Hong-Xia,Qian Chen,Cheng Man-Huan
In this study, based on depolymerization solution of commercially available sodium lignosulfonate under mild conditions, an efficient method for the chromatographic separation of lignosulfonate depolymerization products was firstly established. The self-built database contains 18 lignin-based standards and the information on their names, formulas, structures, retention times, fragments, and corresponding MS E data was developed. By searching the online spectral data in a novel informatics database, a total of nine aromatic lignin depolymerization products were identified automatically. Thus, a fast standard protocol for detection and identification of real lignin degradation products was preliminary built.
研究领域:
分析化学
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林产化学
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环境科学
✓ 已验证
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Huimin Shao,Xiaoyun Su,Yaru Wang,Jie Zhang,Tao Tu,Xiaolu Wang,Huoqing Huang,Bin Yao,Huiying Luo,Xing Qin
Mycotoxins are widely present from cereal crops to food products, posing a significant risk to food safety and causing serious health and economic losses. Therefore, it is imperative to develop a sustainable and efficient detoxification strategy to address mycotoxin contamination. Herein, a dye-decolorizing peroxidase, Ba DyP, from the white-rot fungus Bjerkandera adusta was successfully expressed as a soluble form in Escherichia coli with the help of molecular chaperones. Purified recombinant Ba DyP could efficiently degrade multiple mycotoxins including aflatoxin B 1 , zearalenone, and deoxynivalenol in the presence of mediators such as Mn 2+ or 1-HBT. Meanwhile, their degradation products were identified using UPLC-MS/MS, including AFB 1 -diol, AFQ 1 , 15-OH-ZEN, HZEN, and C 15 H 18 O 8 . Furthermore, cell survival tests exhibited a significant reduction in the biological toxicity of these degradation products. These findings indicate that the use of dye-decolorizing peroxidase could be a promising approach for the effective removal of multiple mycotoxins in cereal crops and food products.
研究领域:
食品安全
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微生物学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2020
·
IF 3.209
Hongwei Zhao,Ling Sun,Huanhuan Chen,Lili Xiang,Dongying Chen
L-α-Glycerylphosphorylcholine (L-α-GPC) is effective to control the symptoms of cognitive decline for the patients of Alzheimer’s disease. In this study, an HPLC method coupled with a refractive index detector was developed to evaluate the intrinsic stability of L-α-GPC. The separation of L-α-GPC and its major potential degradation products was achieved on a normal-phase silica gel column (4.6 mm × 250 mm) with the mobile phase consisting of methanol–20 mM ammonium formate aqueous solution (pH 3.2) (65:35, v/v) in isocratic mode. The HPLC method was validated satisfactorily with respect to precision, accuracy and robustness. It is found that L-α-GPC is stable under the photolytic, thermal, oxidative and acidic conditions, while relatively sensitive to alkaline condition due to the specific breakage of phosphate ester bond in the moiety of L-α-GPC. A preliminary kinetics study for the alkaline degradation was conducted with the corresponding kinetics parameters obtained. It can be concluded that the developed HPLC method is capable of distinguishing the stability difference between the two phosphate ester bonds characterized on the L-α-GPC chemical structure.
研究领域:
药学
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分析化学
·
神经科学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Zhe Wang,Huimin Hu,Liqin Huang,Fanyu Lin,Shuang Liu,Tong Wu,Njud Saleh Alharbi,Samar Omar Rabah,Yuexiang Lu,Xiangke Wang
Developing convenient and rapid method for eliminating uranium from massive contaminated water under emergency conditions is highly needed. Herein we developed a novel chemical precipitation strategy based on graphene aerogel capsulated with the degradation products of phytic acid (PAGA). With hydrothermal treatment, graphene oxide was twisted and reduced to generate aerogel, acting as an encapsulator. At the same time, phytic acid (PA) was degraded into various compounds and capsulated into graphene aerogel with a high loading capacity of 85 g/g. After adding PAGA into uranium contaminated water, the degradation products were released out of GA skeleton to combined with uranium and form sediments. The elimination capacity reached up to 3550 mg/g within 40 min and the obtained sediments possessed good stability in the following 72 h. PAGA capsule also presented high removal rate in various water systems, making it a kind of versatile material in the elimination of uranium under emergency conditions.
研究领域:
环境科学
·
材料科学
·
放射性污染治理
✓ 已验证
产品关联
DOI
高影响因子
CHEMOSPHERE
·
2023
·
IF 8.8
Qingliang Liu,Jinan Zhu,Lu Wang,Xianshi Wang,Zhuangsong Huang,Feng Zhao,Jing Zou,Yulei Liu,Jun Ma
Microbes play a dominant role for the transformation of organic contaminants in the environment, while a significant gap exists in understanding the degradation mechanism and the function of different species. Herein, the possible bio-degradation of triclosan in microbial fuel cell was explored, with the investigation of degradation kinetics, microbial community , and possible degradation products . 5 mg/L of triclosan could be degraded within 3 days, and an intermediate degradation product (2,4-dichlorophen) could be further degraded in system. 32 kinds of dominant bacteria (relative intensity >0.5%) were identified in the biofilm, and 10 possible degradation products were identified. By analyzing the possible involved bioreactions (including decarboxylation , dehalogenation, dioxygenation, hydrolysis, hydroxylation, and ring-cleavage) of the dominant bacteria and possible degradation pathway of triclosan based on the identified products, biodegradation mechanism and function of the bacteria involved in the degradation of triclosan was clarified simultaneously. This study provides useful information for further interpreting the degradation mechanism of organic pollutants in mixed flora by combining analysis microbiome community and degradation pathway.
研究领域:
环境科学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Toxins
·
2022
·Vol. 14
·期 10
·695
·
IF 5.075
Enjie Diao,Kun Ma,Minghua Li,Hui Zhang,Peng Xie,Shiquan Qian,Huwei Song,Ruifeng Mao,Liming Zhang
Patulin (PAT) is one of mycotoxins that usually contaminates apple juice, and it is not easily detoxified by cysteine (CYS) at room temperature due to the highly acidic conditions based on the Michael addition reaction. However, it could be effectively degraded by a heating treatment at 120 °C for 30 min in the presence of cysteine. In our study, a total of eight degradation products (DP A–H) were characterized and identified via liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS) in a negative ion mode, and their structures and formulas were proposed based on their accurate mass data. The fragmentation patterns of PAT and its degradation products were obtained from the MS/MS analysis. Meanwhile, the possible reaction mechanisms involved in the degradation of PAT were established and explained for the first time. According to the relation between the structure and toxicity of PAT, it could be deduced that the toxic effects of PAT degradation products were potentially much less than those of PAT-self.Keywords:patulin;cysteine;degradation product;Michael addition reaction;toxic effectKey Contribution:Eight degradation products of PAT by heat-assisted cysteine under highly acidic conditions were characterized and identified, and their toxic effects were predicted based on their structures. The possible reaction mechanisms involved in the degradation of PAT were established and explained.
研究领域:
食品科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Dong Zhang,Shanshan Huang,Qian Wang,Bo Shang,Jianlei Liu,Xiaoting Xing,Yu Hong,Hui Liu,Xiaoliang Duan,Hui Sun
Brown rice exhibits higher nutritional value and attracts more and more attentions; however, lipid alteration in brown rice during aging is poorly understood. In this study, lipidomics and volatilomics were employed to investigate free fatty acids , triglycerides , and volatile oxidative degradation products of lipids in brown rice during accelerated aging for 70 days. The results showed that the total free fatty acids in brown rice increased significantly (2.90–4.14 times) while triglycerides decreased remarkably at the initial stage of aging. Monounsaturated and polyunsaturated aldehydes, ketones, and acids increased obviously in brown rice during accelerated aging for 70 days. The screening of significantly different compounds indicated that the enzymatic hydrolysis of triglycerides (EHT) and enzymatic oxidation of lipids (EOL) were the main biochemical behaviors at the initial stage of aging (0–28 day) while automatic oxidation of lipids (AOL) was the primary chemical reaction for 28–70 days aging.
研究领域:
食品科学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2018
·
IF 6.434
Lu Wang,Lexing You,Jiaming Zhang,Tao Yang,Wei Zhang,Zhongxiang Zhang,Pinxing Liu,Song Wu,Feng Zhao,Jun Ma
Sulfadiazine (SDZ) is a high priority sulfonamide antibiotic and was always detected in environmental samples. This study explored the removal of SDZ in microbial fuel cells (MFCs), in terms of MFC operation, degradation products, reaction mechanism, SDZ biotoxicity removal, and the correlation between microbial community and SDZ removal. SDZ would greatly impact the activity of reactor microbes, and longtime acclimation is required for the biodegradation of SDZ in MFCs. After acclimation, 10 mg/L of SDZ could be removed within 48 h. Liquid chromatographic-mass spectroscopic analysis showed that SDZ could be degraded into 2-aminopyrimidine, 2-amino-4-hydroxypyrimidine and benzenesulfinic acid. Compared with published SDZ biodegradation mechanism, we found that the sulfanilamide part (p-Anilinesulfonic acid) of SDZ would be degraded into benzenesulfinic acid in the system. The effects of background constituents on SDZ biodegradation were explored, and co-existed humic acid (HA) and fulvic acid (FA) could accelerate the removal of SDZ in MFCs. After analyzing the reactor microbial community and the removal of SDZ at different operation cycles, it was found that the relative abundance of Methanocorpusculum, Mycobacterium, Clostridium, Thiobacillus, Enterobacter, Pseudomonas, and Stenotrophomonas was highly correlated with the removal of SDZ throughout the experiment.
研究领域:
环境科学
·
微生物学
✓ 已验证
DOI
高影响因子
CHEMOSPHERE
·
2018
·
IF 4.427
Qi Han,Wenyi Dong,Hongjie Wang,Tongzhou Liu,Yu Tian,Xin Song
This study systematically investigated the degradation of tetrabromobisphenol A (TBBPA) by ferrate (VI) oxidation. The reaction kinetics between ferrate (VI) with TBBPA were studied under pseudo-first-order conditions in the pH range 5.5–10.5. Then, a series of batch experiments were carried out to investigate other factors, including the ferrate (VI) dosage, temperature and interfering ions. Additionally, the generation of inorganic products (bromide ion and bromate) was evaluated. The organic intermediates were identified, and possible pathways were proposed. In addition, the toxicity variation was analyzed with marine luminous bacteria ( V. fischeri ). Degradation of TBBPA by ferrate (VI) oxidation was confirmed to be an effective and environmentally friendly technique. The reaction was fitted with a second-order rate model. With a ferrate (VI) dosage of 25.25 μmol/L, TBBPA concentration of 1.84 μmol/L, an initial pH of 7.0, and a temperature of 25 °C, a 99.06% TBBPA removal was achieved within 30 min. The evaluation of inorganic products showed that the capacity of ferrate (VI) oxidation to yield bromide ions was relatively strong and could prevent the formation of bromate compared to photocatalytic and mechanochemical techniques. Eleven intermediates were identified, and the proposed degradation pathway indicated that TBBPA might undergo debromination, beta scission , substitution, deprotonation and oxidation. The results of toxicity testing showed that ferrate (VI) could effectively control the toxicity of the treated samples, although the toxicity increased in the initial reaction stage due to the accumulation and destruction of more toxic intermediates.
研究领域:
环境科学
·
化学工程
·
毒理学
✓ 已验证
DOI
高影响因子
CHINESE CHEMICAL LETTERS
·
2022
·
IF 8.455
Run Huang,Chaoting Guan,Qin Guo,Zhen Wang,Hanping Pan,Jin Jiang
The large consumption and discharge of diclofenac (DCF) lead to its frequent detection in surface water and groundwater, posing great threats to humans and ecosystems. This study explored the oxidation kinetics of DCF by permanganate (Mn(VII)), and expounded the underlying reason for the unusual pH-dependency that was unclear in previous studies. The kinetics of DCF analogues ( i.e. , aromatic secondary amines) by Mn(VII) oxidation were comparatively investigated. Then, a tentative kinetic model involving the formation of an intermediate between Mn(VII) and DCF or its analogues was proposed to fit the pH-rate profile. Since DCF contained two chloro groups , and a carboxyl group which could be ionized by negative electrospray ionization , a precursor ionization scanning approach was used for the first time for detection of N-containing chlorinated oxidation products. New degradation pathways of DCF containing ring opening, carboxylation , carbonylation , electrophilic addition , hydroxylation and dehydrogenation were proposed based on the identified oxidation products. Moreover, it was demonstrated that the introduction of various reducing agents such as Mn(II), Fe(II) and bisulfite significantly improved the oxidation kinetics of DCF by Mn(VII). The positive effects of Mn(II) and Fe(II) were mainly attributed to the accelerated formation of MnO 2 that acted as a catalyst or co-oxidizer contributing to DCF degradation. The presence of bisulfite caused two-stage kinetics, where a sharp drop of DCF concentration followed by a slowdown of DCF removal. In the first stage, potent reactive manganese species ( e.g. , Mn(III), Mn(V), and Mn(VI)) and sulfate radical were generated during reaction of bisulfite with Mn(VII), whereas bisulfite was depleted fast due to excess Mn(VII) concentrations and the system became the Mn(VII)/MnO 2 system in the second stage. These results provide new insight into reaction mechanism of DCF with Mn(VII) as well as propose a feasible strategy for enhancing the treatment of DCF contaminated water by Mn(VII).
研究领域:
环境科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）