ANALYTICA CHIMICA ACTA
·
2023
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IF 6.2
Xinghui Yu,Yongqiang Ma,Siyuan Liu,Chunchun Qi,Weiqi Zhang,Wen Xiang,Zhaoxian Li,Kai Yang,Shaoxian Duan,Xinrao Du,Jian Yu,Yan Xie,Zicheng Wang,Wentao Jiang,Li Zhang,Xiaodong Lin
Post-operative pathogenic infections in liver transplantation seriously threaten human health. It is essential to develop novel methods for the highly sensitive and rapid detection of Staphylococcus aureus ( S. aureus ). Interestingly, the combination of the property of bacteria to secrete hydrogen peroxidase , bacterial metabolism-triggered-chemiluminescence (CL)-based bioassays can be as a candidate point-of-care testing (POCT) for the detection of S. aureus against the CL substrate Luminol and hydrogen peroxide without excitation light sources. Here, a CL-based strategy with stable and visualized CL intensity was fabricated according to a hybrid biomimetic enzyme of copper-Hemin metal-organic framework, which enhances the biological enzyme activity while improving the stability and sensitivity of the assay. By further integrating S. aureus -specific capture and one-step separation of the antibody-modified Fe 3 O 4 NPs (Fe 3 O 4 NPs@Ab), the portable device integrated smartphone enables CL-based POCT for specific detection of S. aureu s in the range of 10 1 –10 6 CFU/mL with a limit of detection as low as 1 CFU/mL. Specifically, S. aureus can be eliminated after detection with high antibacterial efficiency due to the excellent photothermal properties of Fe 3 O 4 NPs@Ab. The developed multifunctional platform has the advantages of simplicity of operation and low cost, indicating great potential in clinical applications.
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高影响因子
MOLECULES
·
2023
·Vol. 28
·期 18
·6559
·
IF 4.6
Huihua Zhang,Chaoyan Zhang,Hui Qu,Fengna Xi
Rapid, highly sensitive, and accurate detection of tumor biomarkers in serum is of great significance in cancer screening, early diagnosis, and postoperative monitoring. In this study, an electrochemiluminescence (ECL) immunosensing platform was constructed by enhancing the ECL signal through in situ growth of platinum nanoparticles (PtNPs) in a nanochannel array, which can achieve highly sensitive detection of the tumor marker carcinoembryonic antigen (CEA). An inexpensive and readily available indium tin oxide (ITO) glass electrode was used as the supporting electrode, and a layer of amino-functionalized vertically ordered mesoporous silica film (NH2-VMSF) was grown on its surface using an electrochemically assisted self-assembly method (EASA). The amino groups within the nanochannels served as anchoring sites for the one-step electrodeposition of PtNPs, taking advantage of the confinement effect of the ultrasmall nanochannels. After the amino groups on the outer surface of NH2-VMSF were derivatized with aldehyde groups, specific recognition antibodies were covalently immobilized followed by blocking nonspecific binding sites to create an immunorecognition interface. The PtNPs, acting as nanocatalysts, catalyzed the generation of reactive oxygen species (ROS) with hydrogen peroxide (H2O2), significantly enhancing the ECL signal of the luminol. The ECL signal exhibited high stability during continuous electrochemical scanning. When the CEA specifically bound to the immunorecognition interface, the resulting immune complexes restricted the diffusion of the ECL emitters and co-reactants towards the electrode, leading to a reduction in the ECL signal. Based on this immune recognition-induced signal-gating effect, the immunosensor enabled ECL detection of CEA with a linear range of 0.1 pg mL−1to 1000 ng mL−1with a low limit of detection (LOD, 0.03 pg mL−1). The constructed immunosensor demonstrated excellent selectivity and can achieve CEA detection in serum.
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高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Kanglin Pei,Jianyang Xu,Di Wu,Lin Qi,Lingyan Ma,Renwen Zhang,Wenjing Qi
A fluorescent dual-emitting platform for fluorescent “turn-on” ratiometric detection of ascorbic acid in beverages was developed utilizing luminol-embedded iron-based metal–organic frameworks (luminol@Fe-DOBDC MOFs). Luminol@Fe-DOBDC MOFs with fluorescent emissions at 430 nm and 540 nm under excitation wavelength of 365 nm were applied to detect ascorbic acid on the basis of ascorbic acid triggering the reduction of Fe 3+ into Fe 2+ . In the presence of ascorbic acid, fluorescent intensity at 540 nm was increased significantly while fluorescent intensity at 430 nm was changed slightly. Two emission peaks separated by 110 nm can eliminate environmental interferences by built-in self-calibration of ratiometric signal, enhancing the sensitivity and accuracy. The increasing ratiometric fluorescent intensity ( I 540 nm / I 430 nm ) has linear relationship with the concentration of ascorbic acid from 0.2 to 30 μM with limit of detection of 70 nM. It is an efficient, sensitive and accurate platform to detect ascorbic acid in commercial beverages using transition-metal-based MOFs.
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高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2023
·
IF 19
Wenxiong Cao,Shuang Xie,Yuan Liu,Pan Ran,Zhanlin Zhang,Qibo Fang,Xiaohong Li
Thrombus therapy is challenged by potential bleeding risk of thrombolytic agents, and external irradiation is usually needed in thrombus imaging. Herein, highly efficient theranostic platforms are designed for piezoelectric-Fenton-photodynamic thrombolysis and endogenous thrombus imaging in response to the elevated shear stress at the thrombus site. Piezoelectric tetragonal barium titanate (tBT) nanoparticles (NPs) are coated with Fe 3+ -coordinated metal-organic frameworks (MOFs), followed by inoculation of chlorin e6-luminol conjugates and heparin absorption to obtain theranostic tBT@MOF CL /Hep NPs. Shear stress-induced piezopotential on NPs accelerates Fe 3+ /Fe 2+ transformation to deplete MOF layers, activates Fenton reaction and produces reactive oxidative species (ROS) for non-pharmacological thrombolysis. In the meantime, the site-specific ROS generation oxidizes luminol and the chemiluminescent resonance energy transfer with chlorin e6 generates red fluorescence for thrombus imaging without using external light irradiation. After intravenous injection into a carotid artery thrombosis model, the heparin-mediated thrombus targeting yields 6.5-fold higher luminescent intensity at the thrombus site. Thrombi in the carotid arteries are almost completely dissolved with integration of piezoelectric dynamic therapy, piezopotential-enhanced Fenton reaction, and chemiluminescence-excited photodynamic therapy, while causing no haematologic and histopathologic abnormality. This study demonstrates a concise strategy to generate shear stress-responsive built-in piezoelectric potentials, site-specific non-pharmacological thrombolysis, self-illuminating, and stenosis degree-adaptable thrombus imaging.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Dehua Hu,Lina Yang,Suimin Deng,Yun Hao,Kaina Zhang,Xiangfeng Wang,Yuan Liu,Hailing Liu,Yiping Chen,Mengxia Xie
A sensitive and specific multiplex immunoassay for the biomarkers of hepatocellular carcinoma (HCC), Alpha-fetoprotein (AFP) and Golgi protein 73 (GP73), has been developed. The enzymes, Horse radish peroxidase (HRP) and Alkaline phosphatase (ALP) were modified with active reactive groups of click chemistry, and then self-assembled to form enzyme aggregates, HRPn and ALPn, respectively. The carbon nanotubes (CNT) were conjugated with the enzyme aggregates and the detection antibody of biomarkers by bioorthogonal reaction, forming the immuno nanosensor for AFP and GP73 individually. The enzymes on the nanosensor can catalyze its substrate to emit chemiluminescences, which can be read out in different time windows. Owing to the high reaction efficiency of the bioorthogonal reaction, large capacity of the catalytic enzymes has been conjugated to the surface of CNT, and then the signal intensity of chemiluminiscence has been greatly amplified. The multiplex immunoassay has been successfully applied to detect the biomarkers in real serum samples, which can improve the accuracy and preciseness for the clinical diagnosis of HCC. The nanosensors have high specifity for the biomarkers of HCC, and their sensitivities can be adjustable according to practical demand. This work provides a novel clue to develope immunoassay for clinical diagnosis of HCC.
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高影响因子
ACS Applied Bio Materials
·
2021
·
IF 0
Sheng-Yan Yin,Wei Liu,Ke Zhang,Jishan Li
Current photodynamic therapy (PDT) faces several intrinsic limitations, including insufficient oxygen supply and limited penetration of external light sources. Herein, we report a nanoconjugate, which, in response to the elevated hydrogen peroxide levels associated with tumor tissues, can supplement the oxygen needed for PDT and provide local self-illumination. Consisting of a MnFe2O4 core, a metal–organic framework shell loaded with the chemiluminescence reagent luminol, and a hyaluronic acid surface coating, the nanoconjugate is highly effective for suppressing cancer tissues in vivo via PDT in the absence of externally delivered light.
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ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Wenling Gu,Xiaosi Wang,Jing Wen,Shiyu Cao,Lei Jiao,Yu Wu,Xiaoqian Wei,Lirong Zheng,Liuyong Hu,Lizhi Zhang,Chengzhou Zhu
Luminol-dissolved O2 electrochemiluminescence (ECL)-sensing platforms have been widely developed for sensitive and reliable detection, while their actual ECL mechanisms are still in controversy due to the involved multiple reactive oxygen species (ROS). Different from the structural complexity of nanomaterials, well-defined single-atom catalysts (SACs) as coreaction accelerators will provide great prospects for investigating the ECL mechanism at the atomic level. Herein, two carbon-supported nickel SACs with the active centers of Ni–N4 (Ni–N4/C) and Ni–N2O2 (Ni–N2O2/C) were synthesized as efficient coreaction accelerators to enhance the ECL signals of a luminol-dissolved O2 system. By modulating the surrounding environment of the center metal atoms, their corresponding oxygen reduction behaviors can be well controlled to selectively produce intermediate ROS, giving a great chance to study the following ECL process. According to the experimental and calculated results, the superoxide radical (O2•–) acts as the main radical for the ECL reaction and the Ni–N4/C catalyst with the four-electron pathway to activate dissolved O2 is preferential to enhance ECL emission.
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ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Xiaoqing Sun,Jing Lei,Yan Jin,Baoxin Li
Most of the known chemiluminescence (CL) systems are flash-type, whereas a CL system with long-lasting and strong emission is very favorable for accurate CL quantitative analysis and imaging assays. In this work, we found that the oxidized g-C3N4 (g-CNOX) could trigger luminol-H2O2 to produce a long-lasting and intense CL emission. The CL emission lasted for over 10 min and could be observed by the naked eye in a dark room. By means of a CL spectrum, X-ray photoelectron spectra, and electron spin resonance spectra, the possible mechanism of this CL reaction was proposed. This strong and long-duration CL emission was attributed to the high catalytic activity of g-CNOX nanosheets and continuous generation of reactive oxygen species from H2O2 on g-CNOX surface. Taking full advantage of the long-lasting CL property of this system, we proposed one “non-in-situ mixing” mode of CL measurement. Compared with the traditional “in-situ mixing” CL measurement mode, this measurement mode was convenient to operate and had good reproducibility. This work not only provides a long-lasting CL reaction but also deepens the understanding of the structure and properties of g-C3N4 material.
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Hao Wu,Jun Wu,Hongyong Wang,Yaling Liu,Guoqing Han,Pei Zou
Malathion is one of the most commonly used organophosphorus pesticides that can cause serious harm to the ecological environment and human health. Herein, we demonstrated a label-free chemiluminescent aptasensor for the sensitive detection of malathion based on exonuclease-assisted dual signal amplification and G-quadruplex/hemin DNAzyme. Upon the addition of malathion, the aptamer probe specifically bound to the target to form a complex malathion-S3, leaving a duplex S1-S2. The complex malathion-S3 was digested by exonuclease I and the target was released. The released target was recycled to perform exonuclease I-assisted signal amplification. Furthermore, after treatment with exonuclease III, the duplex S1-S2 was converted into the secondary target ST. The secondary target ST interacted with the hairpin H1 to form a complex H1-ST, which was further digested by exonuclease III and released the secondary target. The released secondary target was recycled to perform exonuclease III-assisted signal amplification. After complete amplification, large numbers of G-quadruplex/hemin DNAzymes were generated. Under the optimal experimental conditions, the prepared aptasensor showed an excellent linear response to malathion with a detection limit of 0.47 pM. The relative standard deviations were in the range of 4.2–6.9%. Moreover, the aptasensor was successfully applied to detect malathion in spiked food and traditional Chinese medicine samples.
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高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Qihao Zhu,Weiran Mao,Chengli Zhang,Yuan Zhou,Zhiyong Tang,Chao Yu
Here, a novel electrochemiluminescence biosensor based on potential-resolved strategy was firstly prepared for the detection of dual targets α2,3-sialylated glycans and α2,6-sialylated glycans. This is the first time that Au@BSA microsphere was used to connect with luminol to enhance its ECL intensity, and it can generate ECL signals at positive potential. Zeolitic Imidazolate Framework-8 (ZIF-8) and Meso-tetra (4-carboxyphenyl) porphyrin (TCPP) were linked using a one-pot method to synthesize a novel luminescent ZIF (L-ZIF) named TZZ, which can emit ECL signals at negative potential. Moreover, magnetite microspheres were used to construct a sandwich-type biosensor to obtain higher sensitivity and reduce background signals. In addition, the biosensor manufactured directly in solution have a wider linear range than constructed on electrode because it has more available space than the electrode surface. Due to the above advantages, the prepared ECL biosensor exhibited high sensitivity, stability and broader linear range, even for practical analysis. Therefore, the prepared ECL biosensor will become a promising method for determination of α2,3-sialylated glycans and α2,6-sialylated glycans in clinical applications in the future. What is more, it provides a potential method for detection of other multi-targets.
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高影响因子
TALANTA
·
2020
·
IF 5.339
Xuejiao Liu,Qiaoe Wang,Jun Chen,Xu Chen,Wensheng Yang
Highly sensitive determination of tumor exosomes is significant for early diagnosis of cancers and precision therapy. Herein, a sandwich peptide-based electrochemiluminescence (ECL) biosensor was developed for determination of phosphatidylserine (PS)-positive exosomes, a promising biomarker for early diagnosis of ovarian malignancy. A PS-specific binding peptide with high affinity was immobilized on Au nanoflowers (AuNFs) modified biosensing interface for recognition and capture of exosomes. Meanwhile, g-C 3 N 4 nanosheet loaded with luminol capped AuNPs (Lum-AuNPs@g-C 3 N 4 ) nanocomposite was used as the ECL signal nanoprobe. The g-C 3 N 4 nanosheets with large surface area were not only utilized as the carrier to immobilize more peptides for recognition of exosomes but also used to catalyze co-reactant H 2 O 2 decomposition to achieve the ECL signal amplification of luminol-H 2 O 2 system. Under optimal conditions, the biosensor showed superior performances compared with most currently available methods, including wider linear range across 5 orders of magnitude and a lower detection limit (LOD) down to 39 particles μL −1 . Moreover, the biosensor could be applicable for determination of exosomes in complex biological samples. This study indicates the combination of peptide recognition with nanoprobe as a label for signal amplification in sandwich ECL biosensing is a great promising strategy for sensitive and cost-effective determination of exosomes.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Yongjun Zheng,Zhenqiang Ning,Deng Pan,Dan Han,Yuan Xu,Songqin Liu,Yuanjian Zhang,Yanfei Shen
Human NAD(P)H:quinone oxidoreductase 1 (hNQO1), a proteinase that engages in detoxification of quinones and capable of activating anti-tumor drugs, has drawn increasing attention as tumor biomarker and drug target. Despite significant advances, the detection of hNQO1 up to now primarily relies on stimulus-responsive probes involving metabolization of a complicated synthetic quinone-functionalized probe, which, however, remains challenging to improve the sensing sensitivity, and are lack of sufficient stability. Herein, we report a highly sensitive electrochemiluminescent detection of hNQO1 and associated drug screening by straightforward sensing of the metabolin, enabled by a futile redox cycle reaction. Thanks to the intrinsic circulatory amplification and the luminol-modified nickel foam electrode, the electrochemiluminescent (ECL) sensor exhibited a record-level detection limit (10 ng/mL) and SNR (∼460) for hNQO1. The same format was also successfully applied to rapidly ranking hNQO1-directed anti-tumor drugs down to several-ten minutes. This new strategy would open a new era for hNQO1 detection with uncompromised high performance and also stimulate ECL as a promising tool that combines both diagnostic and drug screening functions for the popularization of proteinases in cancer management in a future application.
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高影响因子
危险品化学品经营许可证（带存储）