JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
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2018
·Vol. 55
·期 12
·4719-4734
·
IF 1.797
Harlina Putri Widyanti,Ma Meihu,Shahzad Raheel,Gouda Mostafa Mahmoud,Qiu Ning
Antioxidant activity, lipid oxidation, fatty acid composition and volatile compounds of duck eggs supplemented with clove extract were monitored over the salting period. The results indicated that application of clove extracts significantly reduced 2-thiobarbituric acid reactive substances and anisidine values as well as the conjugated dienes levels during curing. Clove extracts along with salting time had significant effects on the fatty acid composition. The predominant fatty acid in salted duck eggs supplemented with clove extracts for 14 day of the salting process was oleic acid, followed by palmitic acid and arachidonic acid. Additionally, treated eggs exhibited a higher docosahexaenoic acid content than that of control. The results of SPME GC–MS showed the presence of 46 and 37 volatiles in the treated eggs. Eugenol, as the primary bioactive component of clove, was detected in salted eggs supplemented with clove extracts. In addition, analyzing the results obtained through electronic nose showed that clove extract brought significant changes in salted eggs flavor components.
研究领域:
食品科学
·
化学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Ronglin Wang,Canru Li,Qianlian Li,Sunxian Zhang,Feng lv,Zhiming Yan
In the current study, a novel covalent organic frameworks COF-SCU1 incorporated electrospun nanofibers (PAN@COF-SCU1 nanofibers) was fabricated via a facile electrospinning method and utilized as adsorbent in pipette tip solid-phase extraction (PT-SPE) of tetracycline antibiotics (TCs) from foods. The prepared PAN@COF-SCU1 nanofibers possessed both of the unique characteristics of electrospun nanofibers and COFS-CU1, and thus improving the adsorption capacity of the electrospun nanofibers and preventing the problems of leakage and high pressure caused by directly using the nanosize COFs as adsorbent in PT-SPE. The experiments affected the adsorption and desorption efficiencies, such as the loading ratios of COFS-CU1 in nanofibers, the amount of nanofibers, the matrix pH and desorption solvent, were studied in detail. Eventually, a new pipette tip solid-phase extraction-high performance liquid chromatography (PT-SPE/HPLC) method was proposed for the analysis of three TCs from food. Satisfied linearity for TCs was obtained in the range of 4–70 ng mL −1 . The limits of detection and quantification were ranged from 0.6 to 3 ng mL −1 and from 2 to 10 ng mL −1 , respectively. The interday and intraday precisions (RSD) were all lower than 9%. The proposed PT-SPE/HPLC method was used to determine TCs residues in grass carp and duck samples for the first time. The results could not only explore the availability of PT-SPE in the extraction of TCs in food samples, but also broadened the potential applications of COFs in sample preparation.
研究领域:
分析化学
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食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
VETERINARY MICROBIOLOGY
·
2024
·
IF 2.4
Guangbin Bao,Shinuo Fan,Chunyan Hu,Chen Li,Fei Ma,Guijun Wang,Hongjie Fan,Qing Wang
Duck Tembusu virus (DTMUV) is a newly emerging pathogen that causes massive economic losses to the poultry industry in China and neighbouring countries. Vimentin, an intermediate filament protein, has been demonstrated to be involved in viral replication during infection. However, the specific role of vimentin in DTMUV replication has not been determined. In this study, we found that overexpression of vimentin in BHK-21 cells can inhibit DTMUV replication. Moreover, DTMUV replication was enhanced after vimentin expression was reduced in BHK-21 cells via small interfering RNA (siRNA). Further research indicated that DTMUV infection had no effect on the transcription or expression of vimentin. However, we found that DTMUV infection induced vimentin rearrangement, and the rearrangement of vimentin was subsequently confirmed to negatively modulate viral replication through the use of a vimentin network disrupting agent. Vimentin rearrangement is closely associated with its phosphorylation. Our experiments revealed that the phosphorylation of vimentin at Ser56 was promoted in the early stage of DTMUV infection. In addition, by inhibiting the phosphorylation of vimentin at Ser56 with a CDK5 inhibitor, vimentin rearrangement was suppressed, and DTMUV replication was significantly enhanced. These results indicated that DTMUV infection induced vimentin phosphorylation and rearrangement through CDK5, resulting in the inhibition of DTMUV replication. In summary, our study reveals a role for vimentin as a negative factor in the process of DTMUV replication, which helps to elucidate the function of cellular proteins in regulating DTMUV replication.
研究领域:
病毒学
·
细胞生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Yalei Dai,Jingyun Zhao,Jin Gao,Qianchun Deng,Chuyun Wan,Bin Li,Bin Zhou
Our previous research had witnessed an efficient yet facile desalination method of salted duck egg white (SDEW) based on the reversible nature of gelatin (GEL) under mild conditions. This current study focused on engineering different gel systems based on desalted duck egg white/gelatin mixture (DDG), namely heat- and cold-induced gels, and the gel properties were further studied. Under the condition of small amplitude oscillatory shear (SAOS), the heat-induced DDG mixed gels has more linear viscoelastic regions and higher yield stress value than the cold-set gels. Additionally, the creep and recovery results demonstrated such heat-induced gels were harder but less elastic than that cold-induced gels. The strain hardening behavior of the gels was observed under high-amplitude oscillating shear. In the large amplitude oscillatory shear (LAOS) measurement, the Lissajous curves showed that in the elasticity analysis of these two different mixed gels, the heated hybrid gels were more elastic. As the content of GEL increased, GEL endowed higher water holding capacity of the hybrid gels. Compared with the cold-set gels, we found that the heat-induced mixed gels appeared a dense porous network microstructure, had greater impact on the water retention. In short, the different properties of constructed gels provided a new way for the utilization of desalted duck egg whites and basic data support for the construction of gel foods.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
VIRUS RESEARCH
·
2015
·
IF 2.324
Yun Chen,Ling Zeng,Wen Xiong,Meiyun Song,Hongxu Du,Yixuan Wang,Ke Ming,Yi Wu,Deyun Wang,Yuanliang Hu,Jiaguo Liu
In our previous study, Bush Sophora Root polysaccharide (BSRPS) and its sulfate (sBSRPS) exhibited anti-duck virus hepatitis (DVH) abilities as well as anti-oxidative and immuno-enhancement effects. The aim of this paper was to ulteriorly investigate the exact anti-DVH mechanisms of BSRPS and sBSRPS by intervention experiments. Hinokitiol and FK506 were used as the pro-oxidant and immunosuppressant, respectively. The dynamic deaths, oxidative and immune evaluation indexes and hepatic pathological change scores were detected. When was intervened by hinokitiol, sBSRPS still possessed therapeutic effect while BSPRS was useless. Under the condition of immunosuppression, BSRPS lost a part role in treating DVH; however such a role of sBSRPS completely exhausted. These results suggested both anti-oxidative and immuno-enhancement effects of BSRPS played roles in healing DVH, and the former was more crucial; unlike BSRPS, only immuno-enhancement ability of sBSRPS was imperative for its curative effect on DVH.
研究领域:
病毒学
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免疫学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
VACCINE
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2016
·
IF 3.413
Tengfei Ma,Yongxia Liu,Jia Cheng,Yanhan Liu,Wentao Fan,Ziqiang Cheng,Xudong Niu,Jianzhu Liu
To obtain an effective vaccine candidate against duck Tembusu viral (DTMUV) disease which causes egg-drop and great economical loss in the Chinese duck industry, liposome vaccines containing recombinant E protein were prepared and assessed in this study. The recombinant plasmid (PET28a-E) was constructed and transformed into BL21 (DE3) cells to produce E proteins. The recombinant E proteins were purified and entrapped by liposomes through reverse-phase evaporation. Eighty-four cherry valley ducks were randomly divided into seven groups and inoculated intramuscularly at one- or seven-day-old with liposomes-E protein or Freund's adjuvant-E protein vaccine. Blood samples were collected from the first week to the tenth week for serum antibody , plasma for viremia , as well as oropharyngeal and cloacal swabs for virus shedding analyses after being challenged with a 10 2.4 50% tissue culture infective dose (TCID 50 ) of duck Tembusu virus. Results showed that serum antibody level of the liposomes vaccine was higher than the Freund's adjuvant vaccine, and inoculating twice was superior to once; furthermore, the viremia and virus shedding tests also proved that the liposomes vaccine can provide complete protection against DTMUV challenge. These results demonstrated that the liposomes-E protein vaccine could be used as a potential candidate vaccine to prevent DTMUV infection in ducks.
研究领域:
兽医学
·
病毒学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Food Science of Animal Resources
·
2022
·
IF 3.135
Putri Widyanti Harlina,Meihu Ma,Raheel Shahzad,Ibrahim Khalifa
The purpose of our study was to determine the impact of rosemary extract in duckeggs, as determined by in vitro antioxidant capacity, lipidoxidation, fatty acid profiles, and flavor analyses. Three groups of salted duckeggs were compared: A control group and group enriched with 0.1% and0.5% (w/v) rosemary extracts for 28 days of salting. In a time-dependentmanner, the radical scavenging activity and reduction power of eggs with0.5% (w/v) rosemary extract were significantly higher those of thecontrol at 28 days after salting. The fatty acid profiles of salted egg weresignificantly affected by rosemary extract and salting time. Palmitic acid wasthe most abundant fatty acid in salted egg treated with rosemary extract,followed by linoleic acid and arachidonic acid. Furthermore, the treated eggscontained more docosahexaenoic acid than the control ones. And the treated eggsalso have a considerable impact on the lipid oxidation process (primary andsecondary oxidation). As a result, rosemary extract can be used as a naturalantioxidant spice to prevent oxidation and extend the shelf life of eggs duringstorage. Furthermore, flavor research using solid phase microextraction - gaschromatography - mass spectrometry and an electronic nose demonstrated thatadding rosemary extract to salted eggs could give them a distinct flavor.
研究领域:
食品科学
·
营养学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Yao Yao,Yucheng Zhong,Han Li,Tiantian Liu,Na Wu,Shuping Chen,Lilan Xu,Yan Zhao,Yonggang Tu
Ovomucin is a key component in maintaining the viscous nature of egg white and has vast potential in the utilization of food industry. The objectives of the study were to determine the effect of different pH on the extractability and gel properties of ovomucin. After removing lysozyme through ion exchange, the diluted duck egg white was acidified to a narrow pH range (4.50, 4.75, 5.00, 5.25, 5.50, 5.75 and 6.00) to precipitate ovomucin. By comprehensive study of purity, yield, and convenience of large-scale preparation, 4.75 was chosen as the optimal pH to extract ovomucin with the highest purity of 85.17 ± 2.31% and a compromised yield of 4.60 ± 0.06 mg/g duck egg white. A series of self-supporting gels were prepared with freeze-dried ovomucin powder at room temperature upon rehydration and centrifugation, and the gels properties were highly associated with pH. Gel formed around pH 5 was the aggregate of natural globular proteins with lowest hydrophobicity and electrostatic interaction, relatively dense structure and high water-holding capacity (WHC). Stronger acidic conditions disrupted the disulfide bonds and formed a rougher spherical aggregate. Under alkaline conditions, however, the protein gradually unfolded and broke into linear basic polypeptides, thus a porous three-dimensional network was formed. Overall, this study provided insights into the formation mechanism of the “acid-tight/alkali-loose” ovomucin gels, and introduced a potential candidate for the development of bioactive substance delivery materials.
研究领域:
食品科学
·
蛋白质化学
·
材料科学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2016
·
IF 3.138
Ke Ming,Yun Chen,Fangke Yao,Jintong Shi,Jingjing Yang,Hongxu Du,Xunyi Wang,Yixuan Wang,Jiaguo Liu
To screen effective anti-duck hepatitis A virus (DHAV) drugs, we applied STMP-STPP method to prepare phosphorylated Codonopsis pilosula polysaccharide (pCPPS), the phosphorylation-modified product of Codonopsis pilosula polysaccharide (CPPS). The IR spectrum and field emission scanning electron microscope (FE-SEM) were subsequently used to analyze the structure of pCPPS. Several tests were conducted to compare the anti-DHAV activities of CPPS and pCPPS. The MTT method was used to compare the effect of the drugs on DHAV-infected duck embryonic hepatocytes (DEHs), and the Reed-Muench assay was employed to observe changes in the virulence of DHAV. We also applied real-time PCR to examine the relationship between virus replication and the expression of IFN-β. The results indicated that CPPS could not inhibit the replication of DHAV. In contrast, pCPPS increased the virus TCID 50 , inhibited viral replication and, accordingly, increased the survival rate of DEHs infected with DHAV. Because DHAV induced the expression of IFN-β, and the IFN-β expression level was positively associated with the number of DHAV, the reduction of IFN-β expression levels after pCPPS treatment demonstrated a decrease in the number of virus particles. These results indicated that pCPPS, which reduces the number of DHAV, was more effective than CPPS in anti-DHAV activity.
研究领域:
病毒学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Congcong Hu,Can Liu,Xu Zhang,Wei Tong,Yu Yin,Jinquan Liu,Shengyuan Yang
Crystal violet (CV), widely used as a fungicide in aquaculture, is susceptible to enrichment in fish and thus poses a threat to human health. Therefore, there is an urgent need for a simple, sensitive and cost-effective method to detect CV. To this need, duck egg white-stabilized gold-silver nanoclusters (DEW-AuAgNCs) with long wavelength fluorescence emission were synthesized via microwave method in 1 min. The raw materials used were easily available and environmentally friendly. And based on the Fluorescence resonance energy transfer (FRET) between DEW-AuAgNCs and CV, a fluorescence quenching method was established for CV detection. The detection limit of this method was 1.7 × 10 −8 mol/L, which was lower than most of the reported methods. In addition, prominent performance of DEW-AuAgNCs with long wavelength emission can be applied for the detection of CVs in real organism.
研究领域:
分析化学
·
纳米技术
·
环境科学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2023
·
IF 6.8
Beiyu Zhang,Meiling Li,Guangteng Zhou,Xin Gu,Longqiang Xie,Man Zhao,Qingbiao Xu,Gaoming Tan,Niya Zhang
Aflatoxin B 1 (AFB 1 ) is a mycotoxin widely present in animal feed and human food, posing a serious threat to animal and human health. This study was aim to illustrate the mechanism of the protective role of MT against AFB 1 -induced hepatotoxicity, as well as to explore the feasibility of enhancing the tolerance of poultry to AFB 1 by upregulating the expression of hepatic MT. After being exposed to AFB 1 (50 ng/kg) primary duckling hepatocytes, the cell viability, the antioxidant index (SOD and GPx) and the mRNA levels of MT downstream genes ( PTGR , p53 , TrxR , AR and Bcl-2 ) significantly ( p < 0.05) decreased, while the intracellular formation of (AFBO)-DNA adduct content, apoptosis, and MDA content significantly ( p < 0.05) increased. Interestingly, overexpression of MT in primary duckling hepatocytes markedly ( p < 0.05) reversed the detrimental impact of AFB 1 and increased the expression of MT downstream genes. HepG2 cells were applied to study the mechanism how MT works to relieve the hepatic toxicity of AFB 1 . The ZnO-NPs (20 μg/mL) + AFB 1 (20 μg/mL) group significantly ( p < 0.05) increased the cell viability, the expression of NRF2 , NQO1 and SOD , and expression of MT and MTF-1 , as well as significantly ( p < 0.05) decreased LDH, ROS and apoptotic rate, comparing with the AFB 1 group. While joint treatment with AFB 1 and ZnO-NPs, the hepatic toxicity exerted by AFB 1 alone was reversed, along with the translocation of MTF-1 from the cytoplasm to the nucleus and upregulated its expression. Duckling trails were further carried out. A total number of 96 1-day-old healthy Cherry Valley commercial ducklings were randomly allocated according to a 2 by 2 factorial arrangement of treatments with the main factors including oral administration of AFB 1 (0 vs. 40 μg/kg) and dietary supplementation of ZnO-NPs (0 vs. 60 mg/kg) for 7 days. It showed that AFB 1 exposure caused body weight loss ( p < 0.05), impaired liver structure and failure in hepatic function (activity of ALT, AST and concentration of TP and GLU) ( p < 0.05), and decreases in antioxidant capacity(activity of SOD, CAT and concentration of GSH) ( p < 0.05), along with the decrease in hepatic concentration of Zn, increase in expression of apoptosis-related genes and protein CAS3 and mRNA Bcl-2 expression ( p < 0.05), and suppressed mRNA levels of antioxidant-related genes MT , SOD1 , NRF2 , and NQO1 ( p < 0.05). In accordance with the cell test, dietary supplementation with ZnO-NPs mitigated the toxicity exerted by AFB 1 . In conclusion, ZnO-NPs has the protective effects against AFB 1 -induced hepatocyte injury by activating the expression of MTF-1 and the ectopic induction of MT expression, providing detailed information on the detoxification ability of MT on AFB 1 .
研究领域:
毒理学
·
分子生物学
·
动物科学
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兽医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2022
·
IF 6.652
Yan Xu,Yiqun Cheng,Zongshuai Zhu,Haoyu Guo,Anthony Pius Bassey,Tianran Huang,Yongqiang Huang,Ming Huang
The effect of different levels (0.01%, 0.03%, 0.05% and 0.10%) of mulberry leaf ( Morus alba L.) extract (MLE) on the formation of free and bound heterocyclic amines (HCAs) in pan-fried muscovy duck ( Cairina moschata ) patties were investigated for the first time. Precursors of HCAs, oxidant index of patties and phenolic composition of MLE were measured. The total free and bound HCAs levels in control samples were 28.37 ng/g and 1935.65 ng/g, and the inhibition rates were 6.71–68.92% and 6.71–14.05% in MLE treatment groups, respectively, and 0.03% showed the strongest inhibitory effect. OPLS-DA result indicated that MeIQ, 8-MeIQx and Norharman were crucial differential compounds in total HCAs. Furthermore, the addition of MLE significantly inhibited the lipid and protein oxidation of patties ( P < 0.05). Fifteen phenolic compounds , mainly flavonoids , were identified by LC-MS. The mitigation of HCAs and oxidation may be attributed to them. Correlation analysis indicated that the total HCAs content was negatively correlated with precursors but positively correlated with oxidation. In conclusion, MLE helped mitigate HCAs formation during muscovy duck processing and appeared to be a viable natural additive for producing healthier processed meat products .
研究领域:
食品科学
·
营养学
·
毒理学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）