ADVANCED SYNTHESIS & CATALYSIS
·
2026
·Vol. 368
·期 15
·e70681
·
IF 3.7
Yating Zou,Jinghui Zhou,Yongyi Zeng,Zhimiao Luo,Bishuang Chen,Lan Liu,Gang Xu
Ene-reductases (ERs) catalyze stereoselective anti-addition to activated alkenes, a mechanism that intrinsically favors trans products and appears incompatible with alkyne semihydrogenation, particularly for the formation of ( Z )-alkenes. Here, we investigate how ERs achieve both ( E )- and ( Z )-selective alkyne reduction within this conserved catalytic framework. Screening of a 51-member ER library reveals distinct kinetic partitioning between alkyne-to-alkene and alkene-to-alkane reduction steps, governed by differential stabilization of the substrate and intermediate within the active site. Guided by this insight, engineering of OYE3 suppresses over-reduction and enables rapid, selective formation of ( E )-alkenes under optimized reaction conditions. Unexpectedly, selected ERs also produce ( Z )-alkenes from cyano-substituted alkynes. Combined mutagenesis and molecular dynamics analyses exclude noncanonical proton donors and further suggest a substrate reorientation mechanism involving a ~180° flip within the active pocket. Together, these findings point to a unified mechanistic framework that may account for how ERs generate both ( E )- and ( Z )-alkenes from alkynes, and provide a working hypothesis for the origin of stereochemical divergence in ER-catalyzed alkyne reduction.
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Water Research X
·
2025
·
IF 8.2
Weihong Xu,Haibei Li,Zhongwei Yang,Tianjiao Chen,Shuqing Zhou,Yue Zhao,Danyang Shi,Dong Yang,Junwen Li,Min Jin
High turbidity, attributed to suspended solids and colloidal particles, can lead to microbiological control failure during water disinfection. Lignin, a naturally occurring particle suspended in water, may contribute to turbidity. However, its ability to adsorb bacteria and protect them from chlorination remains poorly understood. In this study, we investigated Escherichia coli inactivation by NaClO in the presence and absence of natural particles. Among the five tested microparticles, lignin significantly enhanced E. coli survival under NaClO exposure, diminishing its bactericidal effect. Furthermore, surviving bacteria showed upregulation of gene expression related to energy metabolism and oxidative stress, as indicated by transcriptional analysis and qPCR measurements. These findings were further supported by increased ATP content, decreased ROS levels, and a prolonged lag phase in growth curves, enabling bacteria to rapidly restore their growth activity. Further analysis revealed that E. coli binds to the surface of lignin through hydrophobic interactions involving hydroxyl, methyl, aromatic rings, and ether groups. Lignin exhibited a strong bacterial adsorption capacity, as described by pseudo-second-order (PSO) kinetics and the Freundlich model, leading to reduced membrane permeability to NaClO. Importantly, the protective effect of lignin on bacteria was positively correlated with its hydrophobicity, with greater hydrophobicity resulting in enhanced bacterial protection. These findings suggest that lignin significantly increases E. coli resistance to chlorination, potentially contributing to disinfection failure in water treatment. This study highlights the role of lignin as a potential hazard that compromises microbiological control, offering new insights into chlorination failure caused by turbidity.
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Journal of Sensors
·
2022
·
IF 2.336
Chenchen Zhou,Junjun Fan,Ruinan Tan,Qin Peng,Junhuo Cai,Wangxiang Zhang
Linalool is a well-known volatile compound. It is usually extracted from plants or synthesized by chemical methods. However, there is still no rapid screening method for plants with high linalool content. Therefore, we aimed to establish a method determining linalool content in Osmanthus fragrans by electronic nose (E-nose). The volatile gases of three cultivars of O. fragrans were characterized by gas chromatography-mass spectrometry (GC-MS) and E-nose. Different concentrations of linalool were measured by E-nose. Then, the E-nose data were subjected to principal component analysis (PCA) and linear discriminant analysis (LDA) to establish a discriminant model for determining linalool concentrations. Prediction models based on principal component regression (PCR) and multiple linear regression (MLR) were developed from all sensors and feature sensors to determine linalool concentrations in the three cultivars of O. fragrans flowers. Finally, the linalool concentrations determined by gas chromatography were used to verify the accuracy of the prediction model. The results show that linalool had the highest abundance among the volatile compounds of O. fragrans, and the MLR prediction model had an of 0.992 in calibration sets and an of 0.895 in prediction sets using 10 sensors. This study describes a rapid and accurate method for the detection and quantification of linalool concentrations.
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JOURNAL OF MOLECULAR STRUCTURE
·
2022
·
IF 3.841
Chengcheng Tang,Yiran Song,Zhuang Wang,Da Wang,Xinyu Yao,Yuan Meng,Yang Li,Li Yang
Growing multidrug bacterial resistance has become a life-threatening problem. Polymyxins are a “last resort” antibiotic against Gram-negative bacteria (Gram-ne − ), but exhibit weak activity against Gram-positive bacteria (Gram-po + ) caused by different bacterial membrane structures. Herein, it is proposed that the challenge can be overcome by hydrophobically modified polymyxin E for enhancing the hydrophobic interaction with the membrane. After synthesizing mono-and di-fatty acyl conjugates to Thr residues of polymyxin E, seven new polymyxin analogs were screened out with in vitro activity against Gram-ne − and Gram-po + . Among them, di-caprylic acid-modified (comp. 2 ) and mono-linoleic acid-modified (comp. 3) compounds with the most potent activity against both bacteria. Moreover, the membrane research showed that polymyxin analogs obtain activity against Gram-po + by higher ability to dispute cell membrane and wall than polymyxin E. Additionally, the results showed a trend of increasing hydrophobicity of analogs showing the tendency to rise up at the beginning and declining in later against Gram-po + and gradually decreasing activity against Gram-ne − , hence, we speculated that controlling Log P at 1–3 helps to obtain broad-spectrum antibiotic. Crucially, the maximum tolerated doses (MTD) of analogs were significantly higher than polymyxin E, and reduced the nephrotoxicity. As expected, hydrophobic modification polymyxin E not only enhance the activity of Gram-po + , but also could retain the activity of Gram-ne − . This study offers more insights into the synthesis of new antibiotics based on polymyxin E structure, especially broad-spectrum antibiotics.
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CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Xiangjun Qi,Hongtao Zhao,Lihong Wang,Fengqiang Sun,Xiaorui Ye,Xueji Zhang,Mingwei Tian,Lijun Qu
Emerging underwater exploration suggests the need for subaqueous E-textiles. Large-deformation signal feedback for swimming motions and body thermal protection against hypothermia are urgently desirable. Here, underwater-usable E-textiles with remarkable sensing and electrothermal behaviors are introduced, which are composed of stretchable core-sheath fibers based on liquid metal fabricated via a coaxial wet-spinning strategy. The fibers possess outstanding specific resistance (∼0.05 Ω·cm −1 ) and robust sensing detection range for both strain (∼600% elongation) and pressure (∼30 MPa), as well as a quick response time (30 ms). Meanwhile, the fabricated E-textiles present excellent low-power-driving electrothermal performance (1.5 W, ∼50 °C) even at underwater conditions. Remarkably, the conducting liquid metal circuit can be well reversed even after being entirely broken out of the sensing ranges. For proof of concept, an underwater glove integrating motion sensing, visual danger warning and hypothermia prevention is exhibited, which has potential for application in underwater exploration.
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JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2022
·
IF 3.682
Xifa Lan,Quanling Guo,Zhiwei Liu,Kai Liu,Jinfeng He,Ruyu Li,Haotian Sun,Wenxiu Yao,Longgang Wang
Chemotherapy is a major cancer treatment that uses antitumor drugs to kill fast-growing cancer cells. Many kinds of drug carriers have been developed to deliver and achieve controlled release of small-molecule therapeutic agents. However, many therapeutic agent carriers need complex preparation process. The natural polypeptides may serve as proper drug carriers. More specifically, polymyxin E (PE) is a kind of natural antibiotic lipopeptides. It is commonly used to treat infections caused by multidrug-resistant Gram-negative bacteria. Herein, we present a facile method to prepare DOX-loaded polymyxin E micelles (PE-DOX micelles) to enhance the therapeutic effect of anticancer drug doxorubicin (DOX). The hydrodynamic sizes and zeta potential of the prepared nanomedicine (PE-DOX micelles) were 142.0 nm and 6.47 mV, respectively. The release of DOX from PE-DOX micelles was faster at pH 5.5 than that at pH 7.4. Furthermore, PE exhibited negligible cytotoxicity to A549 cells and HeLa cells within 50 μg/mL, while PE-DOX micelles caused higher cytotoxicity than that of free DOX. Moreover, the intravenously injected PE-DOX micelles showed good biocompatibility and obvious antitumor effect after 14 days’ treatment in vivo. The PE-DOX micelles have great potential to be used as anticancer agent in the future.
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Small
·
2023
·Vol. 19
·期 39
·2301593
·
IF 13.3
Shilei Han,Xinrong Zhi,Yifan Xia,Wenyu Guo,Qingqing Li,Delu Chen,Kangting Liu,Xin Wang
Electronic skin (E-skin) with multimodal sensing ability demonstrates huge prospects in object classification by intelligent robots. However, realizing the object classification capability of E-skin faces severe challenges in multiple types of output signals. Herein, a hierarchical pressure–temperature bimodal sensing E-skin based on all resistive output signals is developed for accurate object classification, which consists of laser-induced graphene/silicone rubber (LIG/SR) pressure sensing layer and NiO temperature sensing layer. The highly conductive LIG is employed as pressure-sensitive material as well as the interdigital electrode. Benefiting from high conductivity of LIG, pressure perception exhibits an excellent sensitivity of −34.15 kPa −1 . Meanwhile, a high temperature coefficient of resistance of −3.84%°C −1 is obtained in the range of 24–40 °C. More importantly, based on only electrical resistance as the output signal, the bimodal sensing E-skin with negligible crosstalk can simultaneously achieve pressure and temperature perception. Furthermore, a smart glove based on this E-skin enables classifying various objects with different shapes, sizes, and surface temperatures, which achieves over 92% accuracy under assistance of deep learning. Consequently, the hierarchical pressure–temperature bimodal sensing E-skin demonstrates potential application in human-machine interfaces, intelligent robots, and smart prosthetics.
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Particuology
·
2021
·
IF 3.067
Youhong Zhang,Yinpei Zhu,Leslie S. Loo,Jianguo Yin,Kean Wang
Molecularly imprinted polymer (MIP) beads imprinted with vitamin E (V E ) were synthesized via a two-step swelling polymerization method, using methacrylic acid (MAA) and other functional monomers together with polystyrene (PS) microspheres as the substrate. In initial trials, different functional monomers were assessed, along with a variety of solvents and imprinting ratios. The MIP MAA sample exhibited the highest performance and was characterized and also assessed with regard to V E adsorption under various experimental conditions. This material exhibited reasonably good sphericity, porosity and stability, along with an excellent adsorption capacity of 46 mg/g. This was much higher than the value for the corresponding non-imprinted polymer made with the same monomer, and 20% higher than that for an acrylamide-based MIP synthesized using a precipitation polymerization method. The core-shell structured MIP MAA also demonstrated fast adsorption kinetics, a low pressure drop and good reusability in fixed bed experiments. The V E adsorption equilibrium, selectivity, kinetics, thermodynamics and regeneration capacity of this material were also examined and are reported herein.
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CHEMICAL RESEARCH IN TOXICOLOGY
·
2020
·
IF 3.184
Yuhe Kan,Lu Chen,Dao Lin,Xinya Bu,Mengwu Mo,Liang Yan,Zhenjun Yang,Longfei Yuan,Li Wu,Yujian He
DNA damage can occur naturally or through environmental factors, leading to mutations in DNA replication and genomic instability in cells. Normally, natural d-nucleotides were selected by DNA polymerases. The template l-thymidine (l-T) has been shown to be bypassed by several types of DNA polymerases. However, DNA replication fidelity of nucleotide incorporation opposite l-thymidine in vivo remains unknown. Here, we constructed plasmids containing a restriction enzyme (PstI) recognition site in which the l-T lesion was site-specifically located within the PstI recognition sequence (CTGCAG). Further, we assessed the efficiencies of nucleotide incorporation opposite the l-T site and l-T lesion bypass replication in vitro and in vivo. We found that recombinants containing the l-T lesion site inhibited DNA replication. In addition, A was incorporated opposite the l-T lesion by routine PCR assay, whereas preference for nucleotide incorporation opposite the l-T site was A (13%), T (22%), C (46%), and G (19%), and no nucleotide insertion and deletions were detected in E. coli cells. In particular, a novel restriction enzyme-mediated method for detection of the mutagenic properties of DNA lesion was established, which allows us to readily detect restriction–digestion of the l-T-bearing plasmids. The study provided significant insight into how mirror-image nucleosides perturb the fidelity of DNA replication in vivo and whether they elicit mutagenic effects, which may help to understand both how DNA damage interferes with the flow of genetic information during DNA replication and development of diseases caused by gene mutation.
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JOURNAL OF CONTROLLED RELEASE
·
2021
·
IF 9.776
Lina Liang,Yan Peng,Liyan Qiu
Inducing mitochondrial malfunction is an appealing strategy to overcome tumor multidrug resistance (MDR). Reported here a versatile mitochondrial-damaging molecule, vitamin E succinate (VES), is creatively utilized to assist MDR reversal of doxorubicin hydrochloride (DOX·HCl) via a nanovesicle platform self-assembled from amphiphilic polyphosphazenes containing pH-sensitive 1H-benzo-[d]imidazol-2-yl) methanamine (BIMA) groups. Driven by multiple non-covalent interactions, VES is fully introduced into the hydrophobic membrane of DOX·HCl-loaded nanovesicles with loading content of 23.5%. The incorporated VES also offers robust anti-leakage property toward DOX·HCl under normal physiological conditions. More importantly, upon release within acidic tumor cells, VES can target mitochondria and result in various dysfunctions including excessive generation of reactive oxygen species (ROS), mitochondrial membrane potential (Δ Ψ m ) loss, and inhibited adenosine triphosphate (ATP) synthesis, which contribute to cell apoptosis and insufficient energy supply for drug efflux pumps . Consequently, the killing-effect of DOX·HCl is significantly enhanced toward drug resistant cancer cells at the optimal mass ratio of DOX·HCl to VES. Further in vivo antitumor investigation on nude mice bearing xenograft drug-resistant human chronic myelogenous leukemia K562/ADR tumors verifies the extremely enhanced anti-tumor efficacy of the dual drug-loaded nanovesicle with the tumor inhibition rate (TIR) of 82.38%. Collectively, this study provides a s safe, facile and promising strategy for both precise drug delivery and MDR eradication to improve cancer therapy.
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ULTRASONICS SONOCHEMISTRY
·
2016
·
IF 4.556
Lingling Zhang,Huan Qi,Zhengxu Yan,Yu Gu,Weiqiang Sun,Abraham Amenay Zewde
The present study evaluated inactivation efficiency of a sonophotocatalytic process using ZnO nanofluids including ultrasonic parameters such as power density, frequency and time. The result showed that inactivation efficiency was increased by 20% when ultrasonic irradiation was combined with photocatalytic process in the presence of natural light. Comparison of inactivation efficiency in photocatalytic, ultrasonic and sonocatalytic processes using Escherichia coli as a model bacteria identified that inactivation efficiencies are shown in the following order: ultrasonic irradiation < sonocatalysis < photocatalysis < sonophotocatalysis. Furthermore, inactivation mechanism of sonophotocatalysis was proposed. Studies of reactive oxygen species (ROS) and zinc ions (Zn 2+ ) release evaluation revealed that ROS play a key role in bacterial inactivation rather than Zn 2+ . Permeability of outer membrane (OM) and inner membrane (IM) of E. coli bacterial cells were studied and exhibited that sonophotocatalysis increased the permeability of OM and IM significantly. The enhanced bacterial inactivation effect in sonophotocatalytic process contributed to acoustic cavitation, sonocatalysis of ZnO and sonoporation phenomenon.
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Applied Materials Today
·
2025
·
IF 6.9
Xin Zhang,Wenqing Hai,Junjie Pan,Ye Zhang,Xiang Chen,Zhihao Li,Wenlong Huang,Jinhua Jiang,Siyi Bi,Huiqi Shao,Nanliang Chen,Guangwei Shao
Multifunctional flexible wearable electronic devices will become an important trend in the future development of intelligent electronics. Concurrently, researchers have also turned their attention to reducing the energy consumption of devices during system operation. Here, a flexible, stretchable plush fabric is first knitted by using core-spun yarn similar to the structure of polar bear fur as the raw material, with full rib stitch and weft inlay stitch as the fabric structure. Furthermore, the e-textiles are developed by direct in situ polymerization of polydopamine (PDA) and polypyrrole (PPy) to endow plush fabrics with electrical conductivity. Thanks to the increased contact area of yarn-to-yarn due to the fluff-like appearance and tight fabric construction, our e-textile has high electrical conductivity (1559 S/cm). On the one hand, the test power consumption is reduced, and on the other hand, the e-textile has excellent sensitivity (GF max = -1.75) and a broad sensing range (30%), making it ideal for monitoring the state of human motion and facilitating communication. Moreover, the e-textile excels in electrothermal conversion capabilities (up to 89.5 °C at 13 V), photothermal conversion efficiency (achieving 109 °C at 200 mW/cm 2 ), and scalable electromagnetic interference (EMI) shielding characteristics within the X-band (22 dB). Consequently, biomimetic design provides a viable pathway for the development of adaptable multifunctional wearable devices.
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