Alexandria Engineering Journal
·
2025
·
IF 6.8
Xiaohui Wang,Yu Liu,Bude Su,Jinhua Tan,Jianxin Shen,Qi Chen,Yu Gou,Ying Zhang
This study presents a novel electrochemical biosensor for the detection of CA19–9, a crucial pancreatic cancer biomarker, utilizing gold nanoparticles (AuNPs) biosynthesized from mint extract. The green synthesis approach yielded well-dispersed, spherical AuNPs with a characteristic surface plasmon resonance peak at 520 nm. Modification of glassy carbon electrodes with these AuNPs resulted in a significant enhancement of electrochemical properties, including a 50 % increase in peak current and a 66.7 % reduction in charge transfer resistance. The biosensor exhibited a wide linear range from 0.1 to 100 U/mL with excellent linearity (R² = 0.998) and achieved a remarkably low limit of detection of 0.05 U/mL. The sensor demonstrated high specificity against common interfering substances and maintained stability over 100 cycles with less than 5 % decrease in performance. Compared to traditional ELISA methods, this biosensor offers rapid detection, minimal sample preparation, and superior sensitivity. The integration of biosynthesized AuNPs not only enhanced the sensor's performance but also aligned with sustainable practices in nanomaterial synthesis. This innovative approach paves the way for the development of sensitive, eco-friendly, and cost-effective diagnostic tools for early pancreatic cancer detection and monitoring.
研究领域:
生物传感器技术
·
癌症诊断
·
纳米材料合成
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2023
·Vol. 148
·期 15
·3518-3523
·
IF 4.2
Hao Gu,Guoxin Dai,Zhongqiu Teng,Lina Geng,Wei Xu
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a universal method for the quantitative analysis of small molecular drugs in therapeutic drug monitoring (TDM). Alternatively, liquid chromatography-miniature mass spectrometry (LC-Mini MS) is a simple operating technique for quantitative analysis. However, the wide chromatographic peaks and long retention times of TDM samples using the LC-Mini MS system deteriorated the accuracy and efficiency of quantitative analysis. Here, an optimized electrospray ionization (ESI) interface setup with a splitter valve and a capillary needle (I.D. 30 μm and O.D. 150 μm) of the LC-Mini MS system was acquired. The chromatographic peaks were narrower and smoother and the retention time was shorter for TDM compounds. Furthermore, a quantitative analysis method for risperidone and the active metabolite 9-hydroxyrisperidone in plasma was developed based on this optimal LC-Mini MS setup. The results showed that the calibration curves of risperidone and 9-hydroxyrisperidone had good linear ranges of 2–100 ng mL−1 (R2 = 0.9931) and 2–100 ng mL−1 (R2 = 0.9915), respectively. Finally, the matrix effects, recoveries and stability of risperidone and 9-hydroxyrisperidone samples were analyzed. The results satisfied the requirements of quantitative validation in routine TDM procedures.
研究领域:
分析化学
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药学
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医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DRUG DELIVERY
·
2019
·
IF 3.829
Tao Liu,Xidong Wu,Shaohua Chen,Peina Wu,Hong Han,Hongbin Zhang,Junzheng Li,Guanxue Li,Siyi Zhang
To obtain a high-efficiency drug and gene co-delivery system to HNE-1 tumor therapy, a polymeric prodrug (PAAs-MTX) with chemotherapeutic sensibilization was synthesized consisting of a GSH-response hyperbranched poly(amido amine) (PAAs) and an antitumor drug of methotrexate (MTX). Then, the targeting molecule to HNE-1 cells, transferrin (Tf), was conjugated to form the Tf-PAAs-MTX. This polymeric prodrug could deliver MMP-9 shRNA plasmid (pMMP-9) again to form the drug and gene co-delivery system of Tf-PAAs-MTX/pMMP-9. The co-delivery system showed the effective drug and gene delivery ability with high cytotoxicity and gene transfection efficiency to HNE-1 cells. Besides that, Tf-PAAs-MTX also showed the chemotherapeutic sensibilization effect, the formulation containing PAAs segments showed much higher cytotoxicity than that of free MTX. Benefiting from the sensibilization effect and MTX/pMMP-9 co-delivery strategy, this Tf-PAAs-MTX/pMMP-9 co-delivery system exhibited the significantly improved therapeutic efficacy to HNE-1 tumor in a combined manner which was confirmed by in vitro and in vivo assays. Moreover, its biocompatibility, especially the blood compatibility was analyzed. This polymeric prodrug provided an easily delivery system combining the drug/gene co-delivery, chemotherapeutic sensibilization and targeting into one single platform, which showed a promising application in nasopharyngeal carcinoma therapy.
研究领域:
肿瘤学
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药剂学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Liangliang Xiang,Wenting Cheng,Jiakang Zhang,Xiaoyue Li,Adeel Khan,Yongxiang Yi,Jinlong Li
Matrix metalloproteinase-9 (MMP-9) has been implicated in various tumor cell invasions and metastases. In light of the limitations of traditional methods for MMP-9 detection, we have constructed a novel biosensor depending on cucurbit[8]uril (CB[8]) -mediated host-guest interactions and a sacrificial iron metal-organic framework (FeMOF). Herein, MMP9-specific peptides modified on the gold bare electrode are bonded to the [email protected] @peptide complex through CB[8] addition. The connection between MMP9-specific peptides and signal peptides via CB[8] provides stability as well as enables the immobilization of FeMOF on the electrode surface . When Fe 3+ from the FeMOF interacts with electrochemical buffer K 4 Fe(CN) 6 , Prussian blue will be generated on the gold electrode surface, and a significantly enlarged current response can be detected. However, in the presence of MMP-9, their peptide substrates are specifically cleaved at the site between serine (S) and Leucine (L), which causes an abrupt decrease in the electrochemical signal. The change of signal can reflect MMP-9 concentration. This sensor can reach an ultrahigh sensitivity with a wide detection range of 0.5 pg⋅mL −1 to 500 ng⋅mL −1 and a low detection limit of 1.30 pg⋅mL −1 . Importantly, this sensor is very simple, relying solely on self-sacrificial label of FeMOF, rather than complex functional materials. Additionally, it has been well used in serum samples, showing attractive potential for practical applications.
研究领域:
生物化学
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分析化学
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生物传感器
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癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL & PHARMACEUTICAL BULLETIN
·
2021
·Vol. 69
·期 1
·59-66
·
IF 1.645
Dong-Dong Li,Pan Yu,Hui Xu,Zhen-Zhong Wang,Wei Xiao,Lin-Guo Zhao
Berberine (BBR), a kind of quaternary ammonium benzylisoquinoline alkaloids with multiple pharmacological activities, has been regarded as a promising lipid-lowering agent in the field of drug repurposing. Particularly, the chemical modification at the C-9 position of BBR can remarkably improve its lipid-lowering efficacy. In this study, thirteen novel BBR derivatives were rationally designed, synthesized, and evaluated by preliminary pharmacological tests. The results showed that most compounds exhibited more potent hypolipidemic activities when compared with BBR and simvastatin. Among these compounds, compound 2h-1 and 2h-2 exhibited better activity profiling in these four tests involving with inhibition of total cholesterol (TCHO), triglyceride (TG), and low-density lipoprotein cholesterol (LDLC) and the increase of high-density lipoprotein cholesterol (HDLC). Correspondingly, the BBR analogs with 9-O-cinnamic moiety probably exhibited potent lipid-lowering activity, and should be exploited as an important versatile template for the development of BBR-like lipid-lowering agents.
研究领域:
药理学
·
药物发现
·
化学
✓ 已验证
DOI
PubMed
高影响因子
PHYTOMEDICINE
·
2023
·
IF 7.9
Yanghao Li,Xu Yang,Tiantian Bao,Xiaojuan Sun,Xiang Li,Huilin Zhu,Bo Zhang,Tonghui Ma
Background The therapeutic efficacy of liver injuries heavily relies on the liver's remarkable regenerative capacity, necessitating the maintenance of glycose/lipids homeostasis and oxidative eustasis during the recovery process. Astragali Radix, an herbal tonic widely used in China and many other countries, is believed to have many positive effects, including immune stimulation, nourishing, antioxidant, liver protection, diuresis, anti-diabetes, anti-cancer and expectorant. Astragali Radix is widely integrated into hepatoprotective formulas as it is believed to facilitate liver regeneration. Nevertheless, the precise molecular pharmacological mechanisms underlying this hepatoprotective effect remain elusive. Purpose To investigate the improving effects of Astragali Radix on liver regeneration and the underlying mechanisms. Methods A mouse model of 70% partial hepatectomy (PHx) was employed to investigate the impact of Radix Astragali decoction (HQD) on liver regeneration. HQD was orally administered for 7 days before the PHx procedure and throughout the experiment. N-acetylcysteine (NAC) was used as a positive control for liver regeneration. Liver regeneration was assessed by evaluating the liver-to-body weight ratio (LW/BW) and the expression of representative cell proliferation marker proteins. Oxidative stress and glucose metabolism were analyzed using biochemical assays, Western blotting, dihydroethidium (DHE) fluorescence, and periodic acid-Schiff (PAS) staining methods. To understand the role of AQP9 as a potential molecular target of HQD in promoting liver regeneration, td-Tomato-tagged AQP9 transgenic mice (AQP9-RFP) were employed to determine the expression pattern of AQP9 protein. AQP9 knockout mice (AQP9 −/− ) were used to assess the specific targeting of AQP9 in the promotion of liver regeneration by HQD. Results HQD significantly upregulated hepatic AQP9 expression, alleviated liver injury and promoted liver regeneration in wild-type (AQP9 +/+ ) mice after 70% PHx. However, the beneficial impact of HQD on liver regeneration was absent in AQP9 gene knockout (AQP9 −/− ) mice. Moreover, HQD facilitated the uptake of glycerol by hepatocytes, enhanced gluconeogenesis, and concurrently reduced H 2 O 2 content and oxidative stress levels in AQP9 +/+ but not AQP9 −/− mouse livers. Additionally, main active substance of Radix Astragali, astragaloside IV (AS-IV) and cycloastragenol (CAG), demonstrated substantial upregulation of AQP9 expression and promoted liver regeneration in AQP9 +/+ but not AQP9 −/− mice. Conclusion This study is the first to demonstrate that Radix Astragali and its main active constituents (AS-IV and CAG) improve liver regeneration by upregulating the expression of AQP9 in hepatocytes to increase gluconeogenesis and reduce oxidative stress. The study revealed novel molecular pharmacological mechanisms of Radix Astragali and provided a promising therapeutic target of liver diseases.
研究领域:
药理学
·
肝病学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Acta Biomaterialia
·
2018
·
IF 6.383
Qiao Tang,Xin Ma,Yi Zhang,Xiang Cai,Wei Xue,Dong Ma
Polymeric prodrugs are of immense interest as anticancer drug-delivery system owing to their superior drug stability during circulation and satisfactory drug loading capacity. However, they are usually less effective than free drugs due to imperfect degradable characteristics or active sites blockage. A polymeric prodrug (HPAA-MTX) with chemotherapeutic self-sensibilization effect consisting of glutathione (GSH)-triggered hyperbranched poly(amido amine) (HPAA) and methotrexate (MTX) was designed and synthesized in this work. This prodrug not only showed better inhibition effect on the tumor cells proliferation compared with free MTX , but also displayed selective sensibilization to tumor cells rather than normal cells. Meanwhile, HPAA-MTX was also explored as a MMP-9 shRNA plasmid delivery vector due to their rich amino group of HPAA, accompanying with MTX for simultaneous inhibiting tumor cells proliferation and migration. As expected, HPAA-MTX possessed excellent gene delivery capacity with significant down-regulation expression of MMP-9 protein and further inhibition of MCF-7 cells migration. Benefiting from the self-sensibilization effect and MTX/MMP-9 co-delivery strategy, this HPAA-MTX/MMP-9 co-delivery system exhibited significantly improved therapeutic efficacy to breast cancer in a combined manner which was confirmed through in vitro and in vivo assays. The strategy established in this study provided a facile “all-in-one” platform to integrate the drug/gene co-delivery strategy and self-sensibilization effect into one single nanocomposite for potential cancer treatment. Statement of significance A cationic polymeric prodrug with chemotherapeutic self-sensibilization effect was designed and showed better inhibition effect on tumor cells proliferation compared with its free drug, as well displayed the selective sensibilization effect to tumor cells rather than normal cells. Moreover, the prodrug could also deliver MMP-9 shRNA plasmid for a combined therapy. As expected, the prodrug possessed excellent gene delivery capacity with significant down-regulation expression of MMP-9 protein and further inhibition of MCF-7 cells migration. Benefiting from the self-sensibilization effect and the drug/gene co-delivery strategy, this prodrug exhibited significantly improved therapeutic efficacy to breast cancer in a combined manner.
研究领域:
肿瘤学
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药剂学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
ORGANIC ELECTRONICS
·
2021
·
IF 3.721
Yanyan Wu,Fang Yuan,Feifei Fu,Chenxing Liu,Bo Jiao,Fanghui Zhang,Zhaoxin Wu
Since the emergence of organic-inorganic hybrid perovskites , the development of perovskite light-emitting diodes (PeLEDs) with green/red emission have made great progress, and the corresponding external quantum efficiency (EQE) has exceeded 20%. However, the research progress of blue-emitting PeLED still has certain challenges. In this article, a multi-cation per-bromine perovskite film is prepared by introducing polymer molecules poly(9-vinylcarbazole) (PVK) in an anti-solvent (chloroform). When the concentration of PVK is optimized to 0.1 mg/mL, a smooth, dense, high-quality film with photoluminescence quantum efficiency (PLQY) up to 20.70% is obtained. The introduction of PVK can assist the formation of perovskite films for interface modification via surface defect passivation . The optimized blue PeLED has a maximum brightness of 3136 cd/m 2 and a maximum EQE of 3.49% at 488 nm. More importantly, the optimized blue PeLED has excellent color stability under high applied voltage up to 12 V or continuous operation.
研究领域:
材料科学
·
光学工程
✓ 已验证
产品关联
DOI
高影响因子
DALTON TRANSACTIONS
·
2020
·Vol. 49
·期 46
·17033-17038
·
IF 4.174
Weichi Liang,Jiali Wen,Yukai Zhao,Meng Zhang,Jiuxing Jiang
A layered aluminophosphate |C14H11NO|2[Al4(HPO4)4F4(H2O)2] (denoted as SYSU-2) with a new topology has been hydrothermally synthesized with N-methyl-9-acridone (NMA) as the organic structure-directing agent. Single-crystal X-ray diffraction analysis reveals that SYSU-2 crystallizes in a triclinic space group P, with the inorganic sheets stacked in an AA sequence. Hydrogen bonds are responsible for the neutral inorganic–organic layer connection. The layer structure of SYSU-2 is constructed by alternating AlO4F2 octahedra and PO4 tetrahedra. The topological analysis of SYSU-2 indicates an independent topology. The NMA layers are self-assembled with π–π interaction. SYSU-2 crystals show interesting dual-band emission fluorescence properties compared with NMA crystals. Under 406 nm UV irradiation, SYSU-2 crystals emit yellow light with two emission bands at 477 and 566 nm, while NMA crystals emit blue light with only one band at 473 nm. The differences may be derived from the difference of stacking orders and distance of NMA molecule layers between the two crystals.
研究领域:
材料科学
·
化学
✓ 已验证
DOI
高影响因子
Catalysts
·
2022
·Vol. 12
·期 7
·688
·
IF 4.501
Guolei Xiao,Weibin Chen,Yaming Cai,Shifan Zhang,Di Wang,Dandan Cai
Layered double hydroxide (LDH) has emerged as a promising electrocatalyst; however, the synthetic method usually requires high temperature and high pressure, and sulfate-intercalated LDH is rarely reported. Herein, the sulfate-intercalated CoFe LDH nanosheets were successfully fabricated at ambient temperature via a facile strategy, using two-dimensional ZIF-9(III) as a template and FeSO4as both etchant and iron source. When the as-prepared sulfate-intercalated CoFe LDH acts as an electrocatalyst, it presents superior electrocatalytic performance for the oxygen evolution reaction (OER), requiring low overpotential (η@10 mA cm−2= 218 mV) with a small Tafel slope of 59.9 mV dec−1in 1.0 M KOH, which compares favorably with commercial RuO2and most reported transition-metal electrocatalysts. The high catalytic activity of CoFe LDH might be ascribed to the large interlayer space distance originating from special SO42−ions and the strong synergistic effects between Fe and Co. This work provides a novel and feasible approach to designing highly efficient electrocatalysts based on advanced LDH materials for OER.Keywords:facile synthesis;sulfate-intercalated CoFe LDH;two-dimensional ZIF-9(III);electrocatalysts;oxygen evolution reaction
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
PLoS One
·
2011
·Vol. 6
·期 6
·e21064
·
IF 4.411
Shunzhen Zheng,Shuang Chang,Jinli Lu,Zhihui Chen,Li Xie,Yu Nie,Bin He,Shengquan Zou,Zhongwei Gu
Background
Hepatocellular carcinoma (HCC) is the third most common cause of cancer related mortality worldwide. 9-Nitrocamptothecin (9NC) is a potent topoisomerase-I inhibitor with strong anticancer effect. To increase the solubility and stability, we synthesized a novel 9NC loaded liposomes (9NC-LP) via incorporating 9NC into liposomes. In the present study, we determined the effects of 9NC and 9NC-LP on in vitro and in vivo , and the underlying mechanisms.
Methodology/Principal Findings
We first analyzed the characteristics of 9NC-LP. Then we compared the effects of 9NC and 9NC-LP on the proliferation and apoptosis of HepG2, Bel-7402, Hep3B and L02 cells in vitro . We also investigated their anticancer properties in nude mice bearing HCC xenograft in vivo . 9NC-LP has a uniform size (around 190 nm) and zeta potential (∼−11 mV), and exhibited a steady sustained-release pattern profile in vitro . Both 9NC and 9NC-LP could cause cell cycle arrest and apoptosis in a dose-dependent and p53-dependent manner. However, this effect was not ubiquitous in all cell lines. Exposure to 9NC-LP led to increased expression of p53, p21, p27, Bax, caspase-3, caspase-8, caspase-9 and apoptosis-inducing factor, mitochondrion-associated 1 and decreased expression of Bcl-2, cyclin E, cyclin A, Cdk2 and cyclin D1. Furthermore, 9NC-LP exhibited a more potent antiproliferative effect and less side effects in vivo . Western blot analysis of the xenograft tumors in nude mice showed similar changes in protein expression in vivo .
Conclusions/Significance
In conclusion, 9NC and 9NC-LP can inhibit HCC growth via cell cycle arrest and induction of apoptosis. 9NC-LP has a more potent anti-tumor effect and fewer side effects in vivo , which means it is a promising reagent for cancer therapy via intravenous administration.
研究领域:
肿瘤学
·
药剂学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2017
·Vol. 8
·期 1
·1-13
·
IF 12.124
Zhang Zhaobin,Hu Ying,Guo Jilong,Yu Tong,Sun Libei,Xiao Xuan,Zhu Desheng,Nakanishi Tsuyoshi,Hiromori Youhei,Li Junyu,Fan Xiaolin,Wan Yi,Cheng Siyu,Li Jun,Guo Xuan,Hu Jianying
Bisphenol A (BPA) is used in the production of plastic but has oestrogenic activity. Therefore, BPA substitutes, such as fluorene-9-bisphenol (BHPF), have been introduced for the production of so-called ‘BPA-free’ plastics. Here we show that BHPF is released from commercial ‘BPA-free’ plastic bottles into drinking water and has anti-oestrogenic effects in mice. We demonstrate that BHPF has anti-oestrogenic activity in vitro and, in an uterotrophic assay in mice, induces low uterine weight, atrophic endometria and causes adverse pregnancy outcomes, even at doses lower than those of BPA for which no observed adverse effect have been reported. Female mice given water containing BHPF released from plastic bottles, have detectable levels of BHPF in serum, low uterine weights and show decreased expressions of oestrogen-responsive genes. We also detect BHPF in the plasma of 7/100 individuals, who regularly drink water from plastic bottles. Our data suggest that BPA substitutes should be tested for anti-oestrogenic activity and call for further study of the toxicological effects of BHPF on human health.
研究领域:
环境科学
·
毒理学
·
生殖生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）