NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 25
·12372-12380
·
IF 3.925
Juan Chai,Luohao Yuan,Shiwei Wang,Tong Li,Mingxue Wu,Zhiwei Huang,Hongfeng Yin
Three metal–organic frameworks {[Cu6(L1)4(DMF)(H2O)2·5H2O]}n (1), {Cu3(L1)2(phen)2(H2O)}n (2) and {[Cu8(μ3-OH)3(L2)4(NO3)(H2O)5]·3H2O}n (3) utilizing nitrogen-containing heterocyclic carboxylate bifunctional ligands (L1 = 5-(4-carboxypyridin-3-yl)isophthalic acid and L2 = 2-(5-carboxypyridin-2-yl)terephthalic acid) were self-assembled hydrothermally and characterized. MOFs 1–3 possess 3D networks which consist of three different types of Cu clusters {Cu2O2(OCO)4}, {Cu2O2N4(OCO)2} and {Cu4O10(OCO)4}, respectively. MOFs 1–3 efficiently catalyze the oxidation of cycloalkanes under mild conditions. Besides, the synthesized MOFs 1–3 exhibited high peroxidase-like activity and could be utilized to catalyze the oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid ammonium salt) (ABTS) to produce green color in the presence of hydrogen peroxide (H2O2). The steady-state kinetics experiments of the oxidation of ABTS catalyzed by MOFs 1–3 were carried out, and the kinetic parameters were obtained. The results indicated that the affinity of MOFs 1–3 towards H2O2 and ABTS was higher than that of the natural horseradish peroxidase (HRP). The as-prepared MOF 1 can be applied for the colorimetric detection of H2O2 with a wide linear range of 0.5 to 200 μM and a limit of detection (LOD) of 0.097 μM. Therefore, the novel Cu-MOFs could be regarded as promising artificial enzyme mimics in catalysis, sensors and environment analysis.
研究领域:
材料科学
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生物化学
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分析化学
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DOI
高影响因子
Frontiers in Nutrition
·
2020
·
IF 3.365
Luo Xuguang,Wang Jin,Chen Haiqiang,Zhou Aimei,Song Mingyue,Zhong Qingping,Chen Hanmin,Cao Yong
Finger citron (Citrus medica L. var. sarcodactylis Swingle) is a traditional Chinese herb and considered as a healthy food. Flavonoids are the major bioactive substances in Finger citron. In this study, the major flavonoids of finger citron (FFC) were purified with AB-8 macroporous resins, and then three of them were identified as diosmetin-6-8-di-C-glucoside, hesperidin and diosmetin-6-C-glucoside, and other two were preliminarily inferred as limocitrol 3-alpha-l-arabinopyranosyl-(1->3)-galactoside and scutellarein 4′-methyl ether 7-glucoside by high-performance liquid chromatography and ultraperformance liquid chromatography to quadrupole time-of-flight mass spectrometry. Further, their antioxidation and antiaging activities were determined in vitro and in vivo. In vitro, chemical assays revealed that the purified FFC had strong antioxidative activity as demonstrated by its strong DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS [2,2-azinobis (3-ethyl-benzothiazoline-6-sulphonic acid) diammonium salt] radical scavenging activities and ORAC (oxygen radical absorbance capacity). In vivo, the purified FFC significantly increased the mean and maximum lifespan of Caenorhabditis elegans by 31.26 and 26.59%, respectively, and showed no side effects on their physiological functions. Under normal and oxidative stress conditions, purified FFC reduced the accumulation of reactive oxygen species (ROS) and malondialdehyde, while increased superoxide dismutase (SOD) and catalase (CAT) enzyme activities in C. elegans. Together, we successfully identified three major substances in purified FFC of finger citron and determined the excellent antiaging activity of FFC, which is attributed to its strong antioxidative activity and effect on homeostasis of ROS.
研究领域:
植物化学
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药理学
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2024
·
IF 2.8
Zheng-ming Qian,Meng-qi Wu,Jing Chen,Qi Huang,Deng-yun Fan,De-qiang Li
Hypericum japonicum is a traditional folk medicine with various bioactivities such as hepatoprotective, antioxidant, and anti-tumorous. The antioxidant effect of H. japonicum is one of the most prominent effects due to its responsibility for many of its activities. To clarify active natural substance, the antioxidant properties of H. japonicum were preliminarily assessed by ferric reducing–antioxidant power (FRAP), 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and Oxygen radical absorbance capacity (ORAC), as well as superoxide dismutase (SOD). Then, a straightforward and effective method named online liquid extraction-high performance liquid chromatography combined with ABTS antioxidant assay and mass spectrometry (OLE-HPLC-ABTS/Q-TOF-MS) was developed to swiftly and directly discover the antioxidants in H. japonicum . Using mobile phase as extraction and separation reagent, coupled with online activity analysis and compounds identification by high-resolution MS, the online system enables rapid screening of natural antioxidant bioactives from complex mixture. By using it, a total of 9 compounds including flavonoids and phenolic acids characterized by retention time, precise mass, and fragmentation ions in MS/MS spectra showed antioxidant action. Finally, the antioxidant and SOD activity of main found active compounds were validated by in vitro experiment assay and molecular docking. In summary, these results suggested that H. japonicum could be considered as a potential source of natural antioxidants, and the online integrated system might become a promising candidate for the natural antioxidants discovery in the future.
研究领域:
药用植物学
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分析化学
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药理学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 29
·18296-18300
·
IF 4.036
Xiaofei Liao,Mengyan Li,Li Zou
In this work, a label-free and sensitive colorimetric sensing strategy for the detection of bleomycin (BLM) was developed on the basis of BLM-mediated activation of G-quadruplex DNAzyme via DNA strand scission. A G-quadruplex based hairpin probe (G4HP) containing the scission site (5′-GT-3′) of BLM at the loop region and guanine (G)-rich sequences at its 5′-end was employed in this protocol. In the presence of BLM, it may cleave the 5′-GT-3′ site of the hairpin probe with Fe(II) as a cofactor, releasing the G-tetrads DNA fragment, which may further bind hemin to form a catalytic G-quadruplex-hemin DNAzyme. The resultant G-quadruplex DNAzyme has notable peroxidase-like activity, which effectively catalyzes the oxidation of 2,2′-azino-bis(3-ethylbenzothiozoline-6-sulfonic acid) (ABTS) by H2O2 to produce the blue-green-colored free-radical cation (ABTS·+). Therefore, the detection of BLM can be achieved by observing the color transition with the naked eye or measuring the absorbance at a wavelength of 420 nm using a UV-Vis spectrophotometer. Attributing to the specific BLM-induced DNA strand scission and the effective locking of G-tetrads in the stem of the G4HP, the colorimetric sensing strategy exhibits high sensitivity and selectivity for detection of BLM in human serum samples, which might hold great promise for BLM assay in biomedical and clinical research.
研究领域:
生物医学工程
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分析化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2016
·
IF 4.758
Gonge Tang,Cailan Zhao,Jie Gao,Hongliang Tan
It is of great importance to simply and sensitively detect hydrogen sulfide (H 2 S) because of its role in various physiological processes as well as its inherent toxicity. In this work, a colorimetric method for H 2 S detection was developed by employing terbium-G-quadruplex-hemin (Tb/G4-hemin) DNAzyme as a peroxidase mimic, which can catalyze H 2 O 2 -mediated oxidation of 2,2′-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) to produce radical cation (ABTS + ). Compared with the G4-hemin DNAzyme promoted by K + and Na + , Tb/G4-hemin DNAzyme exhibits a higher catalytic activity. In the presence of Ag + , the peroxidase-like activity of Tb/G4-hemin DNAzyme can be inhibited significantly owing to the disruption of G-quadruplex structure. However, the addition of H 2 S can effectively suppress such negative behavior by competitive binding with Ag + , leading to the recovery of the peroxidase-like activity of Tb/G4-hemin DNAzyme, which can be reflected by an increase in absorbance signal of ABTS + . The absorbance of ABTS + was enhanced linearly with increasing H 2 S concentration from 20 nM to 2 μM. The detection limit for H 2 S is 13 nM, which is much lower than most of previous methods. Moreover, the proposed method possesses the features of simple preparation, easy reproducibility and good biocompatibility. Given the fine performances and striking properties, we believe that the Tb/G4-hemin DNAzyme would have a great promise for analytical applications.
研究领域:
分析化学
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生物化学
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分子生物学
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2014
·
IF 3.208
Guan-Lin Chen,Song-Gen Chen,Ying-Ying Zhao,Chun-Xia Luo,Juan Li,Yong-Qing Gao
In order to provide new information on the antioxidant activities before and after in vitro digestion of selected fruits for consumers, nutritionists, and food policy makers, total phenolic contents and antioxidant capacities of 33 fruits were evaluated by Folin–Ciocalteu method, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity assay (DPPH), Ferric reducing antioxidant power assay (FRAP), and ABTS •+ radical cation scavenging activity (ABTS) assay, respectively. The correlations between DPPH, FRAP, ABTS values and total phenolic contents were also evaluated. The results showed that different fruits had diverse antioxidant capacities and the variation was very large. Before the gastric and duodenal phases of in vitro digestion, there was a high positive correlation between the DPPH, FRAP, ABTS values, and total phenolic contents. After the digestion, however, the correlations between the results and the total phenolic contents were poor. Four fruits, plum (sanhua) ( Prunus salicina Lindl), red bayberry ( Myrica rubra (Lour.)), plum (green) ( P. salicina Lindl), and mango (shuixian) ( Mangifera indica Linn) showed the strongest antioxidant activities they were before or after the digestion, which implied that these fruits were important natural sources for preventing oxidative stress diseases.
研究领域:
食品科学
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营养学
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生物化学
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产品关联
DOI
高影响因子
JOURNAL OF AOAC INTERNATIONAL
·
2025
·
IF 1.7
Tang Dianchao,Jin Chen,Gan Liqin,Zhong Lanlan,Wang Zexi,Liu Qiqiong,Yang Yiling,Liu Ling,Huang Huilian
BackgroundTinosporae Radix is a valuable plant with applications in medicinal and edible fields. Alkaloids are one of the main active components of Tinosporae Radix, showing prominent antioxidant activity and great potential in healthcare fields.ObjectiveResponse surface method was applied to determine the optimal enrichment process of total alkaloids of Tinosporae Radix (TRTA). Antioxidant alkaloid components were screened by characteristic chromatogram, antioxidant analysis and molecular docking, so as to provide the basis for Tinosporae Radix to develop as a medicinal and edible homologous plant and antioxidant products.MethodsIn this study, a single-factor investigation experiment and a response surface optimization experiment were used to determine the optimal enrichment process of D101 macroporous resin. High-performance liquid chromatography (HPLC) was employed to establish the characteristic chromatogram of TRTA, after which chemometrics was applied to evaluate the data. The antioxidant activity of TRTA was assessed using DPPH and ABTS assays. Grey relational analysis was applied to explore the spectrum-effect relationship, and molecular docking techniques were combined to predict targets of antioxidant activity.ResultsThe best enrichment process of total alkaloids is presented as follows: water removal 3 BV, 10% ethanol removal 3 BV, and 30% ethanol elution 3 BV. And all four identified alkaloid components contributed to the antioxidant activity of TRTA, potentially processing binding interactions with multiple antioxidant protein targets, including TNF, NO-1 and XOD.ConclusionsAlkaloids significantly contributed to the antioxidant activity of TRTA, with columbamine and palmatine playing particularly prominent roles. And the antioxidant activity of TRTA is likely exerted through a complex multi-component, multi-target mechanism, involving the coordinated action of various alkaloid components and multiple biological targets.HighlightsThe optimized enrichment process demonstrated remarkable efficiency in concentrating total alkaloids from Tinosporae Radix. This study contributes significantly to the formulation of its quality control methods.
研究领域:
植物化学
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分析化学
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药理学
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计算生物学
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产品关联
DOI
PubMed
高影响因子
FITOTERAPIA
·
2024
·
IF 2.5
Meihong Liu,Yongxia Jia,Haihui Xie
Michelia champaca L. (Magnoliaceae) was cultivated in large scale for flowers as cosmetic raw materials, whereas the value of its leaves remains to be discovered. Our chemical study on the leaves yielded four new flavonol diglycosides, champaflavosides A–D ( 1 – 4 ), together with twenty-three known flavonoid glycosides ( 5 – 27 ). Their structures were determined by spectroscopic and chemical methods. Compounds 5 – 21 and 23 – 27 were not previously reported from the genus Michelia , and kaempferol 3- O -rutinoside ( 22 ) was obtained from this species for the first time. All the compounds were evaluated for antioxidant activity by four in vitro assays. Compounds 3 – 12 and 20 showed more potent 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity than l -ascorbic acid ( l -AA). Compounds 2 – 23 , 25 , and 27 exhibited 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical cation scavenging activity superior to l -AA. The ferric reducing antioxidant powers (FRAP) of compounds 2 – 13 , 17 , and 19 were higher than l -AA. Further, eighteen compounds demonstrated cellular reactive oxygen species (ROS) scavenging activity, of which champaflavoside D ( 4 ), rhamnetin 3- O -neohesperidoside ( 8 ), quercetin 3- O -(6- O - E - p -coumaroyl)-neohesperidoside ( 9 ), and liquiritin ( 27 ) were more potent than curcumin. The results revealed that the renewable leaves of M. champaca are a rich source of flavonoids and antioxidants.
研究领域:
药用植物学
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生物化学
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植物化学
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产品关联
DOI
PubMed
高影响因子
SOUTH AFRICAN JOURNAL OF BOTANY
·
2021
·
IF 2.315
Si-Yu Zhong,Hong-Yu Jiang,Li-Ping Wu,Meng-Ying Liu,Ya-Rui Yu,Cheng-Rong Li,Jing Huang
A new neolignan, (-)-(7 S , 8 R )-3‑methoxy-4', 7-epoxy-8, 5'-neolignane-5, 9, 3', 9′-tetraol ( 1 ) and a new biphenylpropanoid, 1, 2-epoxy-1-(3', 4'-dihydroxyphenyl-)-3-(2'', 3'', 5''-trihydroxyphenyl-) propane ( 2 ), together with eight known compounds ( 3-10 ), were isolated from the 95% EtOH extract of the leaves of Cupressus funebris Endl . Their structures were determined by spectroscopic methods including NMR, circular dichroism (CD) spectra and comparison of the NMR data with reported in references. The antioxidant activities of all compounds were evaluated by DPPH, ABTS, and FRAP methods. Compounds 2 (IC 50 : 33.98 ± 0.11 μM), 3 (IC 50 : 32.97 ± 0.63 μM), 8 (IC 50 : 32.07 ± 1.57 μM), and 9 (IC 50 : 33.30 ± 2.29 μM) showed stronger DPPH free radical scavenging activity than l -ascorbic acid (IC 50 : 64.31 ± 1.32 μM). Except for 4 , the other compounds showed ABTS radical scavenging abilities comparable to l -ascorbic acid (54.91 ± 1.18 μM) with the IC 50 ranged from 24.42 ± 1.78 μM to 138.70 ± 13.58 μM. Compound 3 (0.72 ± 0.02 mmol/g) showed the same ferric reducing antioxidant power (FRAP) as l -ascorbic acid (0.83±0.02 mmol/g).
研究领域:
药用植物学
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天然产物化学
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生物化学
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产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2023
·
IF 4.1
Bowei Du,Chao Zhang,Guiya Deng,Shuai Zhang,Shiqi Wang,Yuepeng Guan,Yaqin Huang
Background Nowadays, the prevalence of oxidative stress-related chronic diseases is increasing. The identification of novel antioxidant collagen-peptides to counteract oxidative stress for individuals’ health have gained significant intention. Results In this study, the collagen-peptides with antioxidant activities were separated and identified by ion chromatography (IEC), reversed-phase high-performance liquid chromatography (RP-HPLC), and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The identified antioxidant collagen-peptides were further screened by molecular docking for Keap1-targeted peptide inhibitors and their theoretical interaction mechanisms were investigated. Four novel antioxidant collagen-peptides, GPAGPIGPVG, GPAGPpGPIG, ISGPpGPpGPA and IDGRPGPIGPA with high binding affinity to Keap1 were selected. Molecular docking results demonstrated that the putative antioxidant mechanism of four antioxidant collagen-peptides contributed to their blockage of Keap1-Nrf2 interactions. The results of antioxidant activity of four antioxidant collagen-peptides proved that IDGRPGPIGPA exerted a high scavenging capacity to DPPH and ABTS free radicals, while GPAGPpGPIG improved the resistance of cells to hydrogen peroxide (H 2 O 2 ) induced oxidative damage by promoting the activation of intracellular antioxidant enzymes and the production of reduced glutathione in human hepatoma (HepG2) cells. Conclusion The antioxidant collagen-peptides (GPAGPIGPVG, GPAGPpGPIG, ISGPpGPpGPA and IDGRPGPIGPA) will be developed as the novel functional food for human health in the near future. This article is protected by copyright. All rights reserved.
研究领域:
药理学
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生物化学
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分子生物学
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细胞生物学
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食品科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·Vol. 416
·期 8
·1951-1959
·
IF 3.8
Sun Shuo,Chen Changyu,Fu Xingyu,Zhang Yandong,Wu Xinyue,Hao Junkai,Feng Jing,Hu Lei,Yao Wenli,Yan Zhengquan
To conveniently monitor bioactive cysteine (Cys) and Fe 2+ in practice, a kind of poly-β-cyclodextrin strengthen praseodymium oxide (Pr 6 O 11 ) porous oxidase mimic ( p -β-CD@Pr 6 O 11 ) was constructed by virtue of the strong coordination between nano Pr 6 O 11 and poly-β-cyclodextrin substrate. After its microstructure and physicochemical property were characterized in detail, it was noted that porous p -β-CD@Pr 6 O 11 exhibited excellent enzyme-like catalytic activity to accelerate the oxidation of 3,3′,5,5,′-tetramethylbanzidine (TMB) and 2,2′-azinobis (3-ethylbenzo-thiazoline-6-sulfonic acid) ammonium salt (ABTS) with significant color-enhancement effect in the air. Based on the signal amplification, trace Cys could exclusively deteriorate the UV-vis absorbance at 653 nm of p -β-CD@Pr 6 O 11 -TMB and Fe 2+ alter the one at 729 nm of p -β-CD@Pr 6 O 11 -ABTS with visual color changes. Under the optimized conditions, the proposed p -β-CD@Pr 6 O 11 -TMB and p -β-CD@Pr 6 O 11 -ABTS systems were successfully applied for dual-channel monitoring of Cys in Cys capsules and fetal bovine serum and Fe 2+ in agricultural products with quite low detection limits, i.e., 7.8×10 −9 mol·L −1 for Cys and 6.93×10 −8 mol·L −1 (S/N=3) for Fe 2+ , respectively. The synergetic-enhancement detection mechanisms to Cys and Fe 2+ were also proposed. Graphical
研究领域:
分析化学
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材料科学
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生物化学
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纳米技术
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环境监测
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生物传感器
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DOI
高影响因子
Frontiers in Microbiology
·
2025
·
IF 4.5
Xiaomin Zhu,Youren Duan,Jianqi Lu,Wei Xia,Yujia Peng,Jiawei Liu,Weiliang Dong,Min Jiang
Polyurethane (PU), a segmented block copolymer with chemically resistant urethane linkages and tunable architecture, presents persistent biological recycling challenges. This study presents a Bacterial Laccase-Mediated System (BLMS) derived from Bacillus subtilis for efficient degradation of polyester- and polyether-PU. Utilizing the laccase CotA and mediator 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), the BLMS demonstrated effective de polymerization of both commercial and self-synthesized PU foams, including polyester- and polyether-types. The weight loss of the self-synthesized polyester-foam and the commercial polyether-foam reached up to 21.24 ± 1.20% and 3.81 ± 0.36%, respectively. Subsequently, we detected oxygenated products such as ketones, alcohols, aldehydes, acids, esters, ethers, and 2,4– toluenediamine (2,4-TDA) indicating that bacterial laccase CotA exhibited redox catalytic activity toward PU. Moreover, an interesting phenomenon was observed during the degradation process that the solution turned purple. We predicted that this attribute to the enzymatic oxidation of ABTS to the radical cation ABTS·+, which subsequently reacts with 2,4-TDA to form the purple product. This study finds a plastic degrading enzyme capable of hydrolyzing urethane bonds in PU, offering a promising contribution to the development of a bio-based circular economy for PU biodegradation and recycling.
研究领域:
生物降解
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酶催化
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高分子科学
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生物工程
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环境生物技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）