Analytical Methods
·
2023
·Vol. 15
·期 29
·3586-3591
·
IF 3.1
Li Zhang,Jiyan Huang,Mixue Chen,Hongmei Huang,Yi Xiao,Ronghua Yang,Youyu Zhang,Xiaoxiao He,Kemin Wang
Amphiphilic aggregation-induced emission (AIE) molecules show superior potential for fabricating novel ultrasmall nanoprobes. Here, an anionic dipyridyl tetraphenylethene (TPE) derivative is rationally designed and a super-small self-assembled AIEgen nanoprobe (TPE-2Py-SO3NaNPs, ca. 2.48 nm) is thus conveniently constructed for the supersensitive detection of protamine and trypsin. In HEPES/DMSO solution (8 : 2, v/v, pH = 7.4), negatively charged TPE-2Py-SO3NaNPs exhibited an AIE effect in the presence of positively charged protamine, presenting a fluorescence enhancement at 498 nm together with a large Stokes shift of 150 nm and a low detection limit of 8.0 ng mL−1. In addition, the in situ formed TPE-2Py-SO3Na/protamine nanocomposite can be dissociated by trypsin due to the highly selective degradation of protamine via enzymatic hydrolysis, achieving a detection limit for trypsin as low as 5.0 ng mL−1.
研究领域:
生物化学
·
分析化学
·
医学检测
✓ 已验证
DOI
PubMed
高影响因子
Biomaterials Science
·
2020
·Vol. 8
·期 20
·5739-5750
·
IF 6.183
Sihui Wei,Guilan Quan,Chao Lu,Xin Pan,Chuanbin Wu
Photothermal therapy (PTT) based on aggregation-induced emission luminogen (AIEgen) is very promising for superficial tumor therapy due to the superior photostability and photothermal conversion efficiency of AIEgens. However, the systemic administration of AIEgen remains challenging, mainly because of solubility dissatisfaction and biodistribution. Here, a dissolving microneedle (MN) system loaded with AIEgen (NIR950) was developed for topical administration to treat malignant skin tumor melanoma. Firstly, NIR950-loaded polymeric micelles (NIR950@PMs) were prepared via a nanoprecipitation method to increase the drug solubility. Then, micelles were concentrated on needle tips of MN (NIR950@PMs@MN) by a two-step molding method. NIR950@PMs showed no distinct decline in emission intensity under continuous laser irradiation for an hour. Moreover, the pH-responsive micelles can be protonated in an acidic tumor microenvironment to facilitate the intracellular uptake. By virtue of dissolving MN, NIR950@PMs could rapidly accumulate at the tumor site and reach a suitable temperature for killing cancer cells under laser irradiation. With only single administration and one-time laser irradiation, the NIR950@PMs@MN could notably eliminate melanoma tumors with a low dose of NIR950. Overall, this dissolving MN system loaded with NIR950 showed remarkable photostability and also achieved a valid photothermal effect, which indicate great potential for clinical superficial tumor therapy.
研究领域:
药学
·
生物医学工程
·
癌症治疗
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
Science China-Chemistry
·
2021
·Vol. 65
·期 2
·339-352
·
IF 9.445
Wang Yipu,Mei Dong,Zhang Xinyi,Qu Da-Hui,Mei Ju
Precise and early detection of β -amyloid (A β ) deposits in situ and in real time is pivotal to the diagnosis and early intervention of Alzheimer’s disease (AD). Optical imaging stands out to be a promising technique for such a task; however, it still remains a big challenge, due to the lack of high-performance imaging contrast agent. Restricted by poor blood-brain barrier (BBB) penetrability, short-wavelength excitation and emission, as well as the aggregation-caused quenching effect, the widely used gold-standard probes cannot be used for early in-vivo imaging of A β deposits. Herein, we integrate the A β deposits-favored geometry, amphiphilic and zwitterionic molecular structure, extended D-π-A electronic structure, and 3D conformation into one molecule, facilely establishing a simple and economic imaging contrast agent that enjoys high specificity and affinity to A β deposits, good BBB penetrability, bright red/near-infrared fluorescence, low interference from autofluorescence, aggregation-induced emission (AIE) feature, high signal-to-noise ratio (SNR), and high contrast. In-vitro, ex-vivo , and in-vivo experiments with different strains of mice indicate that AIE-CNPy-AD holds the universality to A β deposits identification. Noteworthily, AIE-CNPy-AD is even able to precisely trace the small and sparsely-distributed A β deposits in AD model mice as young as 4-month-old APP/PS1 mice, the youngest having A β deposits. Moreover, the present probe could clearly reveal the increase and enlargement of A β deposits as the mice grow. Therefore, AIE-CNPy-AD might be an ideal alternative for early AD diagnosis and highly reliable monitoring of AD progression.
研究领域:
神经生物学
·
医学影像学
·
分子探针设计
✓ 已验证
DOI
高影响因子
Chem
·
2024
·
IF 19.1
Xiaoze Wang,Xia Wang,Jianxin Song,Hongliang Bao,Mengjia Yuan,Yanlong Wang,Lingxin Kong,Wei Liu
Developing materials capable of converting high-energy photons into visible (vis) or ultraviolet (UV) light serves as an effective approach for achieving X-ray detection and imaging. In this work, highly luminescent metal-organic gel (MOG), YTU-G-1, was obtained by self-assembling the aggregation-induced emission luminogen (AIEgen)-functionalized ligand and Zr oxo-hydroxy clusters via a mild solvothermal method. The dense and rigid MOG skeleton offers the opportunity to maximize the AIE effect, achieving near-unity quantum efficiency of 95.5%. Under X-ray irradiation, YTU-G-1 exhibits bright luminescence and can detect low-dose radiation down to 44 nGy s −1 . The robust Zr–O bond imparts the material with excellent chemical and physical resistance, making it an ideal candidate for fabricating large-area flexible membranes. Consequently, the spatial imaging resolution of the flexible scintillating membrane reaches 20 lp mm −1 . This work offers a general strategy for fabricating scintillators based on MOGs and facilitates the construction of flexible X-ray imaging membranes.
研究领域:
材料科学
·
医学影像
·
辐射检测
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2017
·
IF 7.504
Qing-Lan Li,Duo Wang,Yuanzheng Cui,Zhiying Fan,Li Ren,Dongdong Li,Jihong Yu
A novel multifunctional drug delivery system has been constructed by assembling per-6-thio-β-cyclodextrin-modified ultrasmall CuS nanoparticles (CD-CuS) onto fluorescent AIEgen-containing mesoporous silica nanoparticles (FMSN). The CD-CuS nanoparticles are anchored on the surface of benzimidazole-grafted FMSN, acting as a gatekeeper and photothermal agent. The prepared blue-emitting nanocomposite (FMSN@CuS) exhibits good biocompatibility and cell imaging capability. Anticancer drug doxorubicin hydrochloride (DOX) molecules are loaded into FMSN@CuS, and zero prerelease at physiological pH (7.4) and on-demand drug release at an acidic environment can be achieved due to the pH-responsive gate-opening of CD-CuS only at an acidic condition. The FMSN@CuS nanocomposite can generate obvious thermal effect after the exposure of 808 nm laser, which can also accelerate the DOX release. Meanwhile, the fluorescence intensity of DOX-loaded FMSN@CuS increases with the release of DOX, and the intracellular drug release process can be tracked according to the change of luminescence intensity. More importantly, DOX-loaded FMSN@CuS displays efficient anticancer effects in vitro upon 808 nm laser irradiation, demonstrating a good synergistic therapeutic effect via combining enhanced chemotherapy and photothermal therapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Chengchen Pang,Keyun Ren,Haitao Xu,Kunying Nie,Chunlei Yu,Daohong Zhang,Qingqing Yang
To enable on-site detection of ZEN toxin in agricultural samples, an POC LFIA detection method based on monoclonal antibody was established through an advanced MAF materials. AIEgens have garnered significant attention for their unique fluorescence properties at high concentrations, overcoming the ACQ limitation of traditional luminescent materials. MAF is a Zr⁴⁺-based AIE metal-organic framework that amplifies AIEgen fluorescence and enables crosslinker-free antibody assembly through its intrinsic affinity for antibodies. This preserves antibody activity while ensuring detection sensitivity through targeted Fab exposure. The MAF-LFIA exhibited good linearity with an ultralow limit of detection of 0.0021 ng/mL (approximately 0.42 ng/g in corn samples), a cut-off value of 5 ng/mL, and recoveries of 99.9-102.3% in cornmeal. These results indicated that this platform provides a robust strategy for real-time monitoring of food contaminants in complex matrices.
研究领域:
分析化学
·
食品安全
·
生物传感器
·
免疫分析
·
纳米材料
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Huanhuan Xing,Sipeng Tian,Zihan Zhou,Zihang Zhang,Chunmei Zhang,Shan Zhang,Jian Lin,Chunxian Guo,Erkang Wang,Jing Li
The design and synthesis of high-efficiency electrochemiluminescence (ECL) emitters hold great promise for a wide range of analytical applications. In this study, we developed a rapid and straightforward strategy to fabricate a self-luminous Cd-based metal–organic framework (Cd-MOF) using individual aggregation-induced emission ligands, specifically 1,1,2,2-tetrakis(4-(pyridin-4-yl)phenyl)ethane (TPPE), within a few seconds. The rigid and directionally enriched metal node of Cd, along with the organic ligands, is formed within the Cd-MOF via coordination, effectively constraining the intramolecular free motions of TPPE and suppressing nonradiative relaxation. Additionally, the unique porous structure combined with the catalytic activity resulting from the incorporation of Cd2+, endow the Cd-MOF with 90-fold ECL enhancement compared to individual TPPE as more chromophores are electro-excited and more coreactants are catalyzed to produce luminescence. The as-made Cd-MOF amplifies the ECL performance by integrating ECL emitters and coreactant accelerators into a single entity, simplifying the sensing process. Leveraging the excellent ECL performance, we constructed a sensitive ECL sensor for hydroquinone based on competitive reactions, with a wide linear range from 200 nM to 1 mM and a satisfying detection limit as low as 80 nM.
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
·
2023
·Vol. 13
·期 3
·351
·
IF 5.4
Lei Liu,Huixing Wang,Husseini Sulemana,Bing Xie,Li Gao
As a biomarker, alpha-fetoprotein (AFP) is valuable for detecting some tumors in men, non-pregnant women, and children. However, the detection sensitivity in some methods needs to be improved. Therefore, developing a simple, reliable, and sensitive detection method for AFP is important for non-malignant diseases. An aptamer binding was developed based on aggregation-induced emission luminogen (AIEgen) nanosphere labeled with Fe3O4@MPTMS@AuNPs. AFP was detected with a sandwich structure of AuNPs magnetic composite particles. An aggregation-induced emission (AIE) molecule and polystyrene (PS) nanosphere complex were assembled, enhancing the fluorescence and improving the sensitivity of detection. The limit of detection (LOD) was at a given level of 1.429 pg/mL, which can best be achieved in serum samples. Finally, the results obtained showed the complex to be promising in practical applications.Keywords:aptamer;AFP;detection;AIE
研究领域:
生物化学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Panyi Hu,Liping Su,Jinfeng Liu,Rui Zhao,Dong Wang,Jiahan Cheng,Xiaohe Tian
Reliable visualization of lipid droplet (LD) remodeling remains challenging because conventional LD probes often suffer from background fluorescence and limited photostability in lipid-rich cellular environments. Here, we developed LD-TTP, an LD-targeting fluorescent probe integrating aggregation-induced emission (AIE) and intramolecular charge transfer (ICT) properties. LD-TTP exhibits strong fluorescence activation, high selectivity, and excellent photostability, enabling robust imaging and quantitative analysis of LD dynamics in cell-based steatotic models. Using staged steatotic HepG2 cells, we found that LD remodeling was accompanied by stage-dependent changes in the spatial distribution of the amyloid-β (Aβ)-associated receptor low-density lipoprotein receptor–related protein 1 (LRP1). In early steatosis, LRP1 expression increased and became enriched around LDs, coinciding with enhanced uptake of exogenous Cy5-labeled Aβ. As steatosis progressed, LRP1 expression declined, and its LD-associated localization was lost, paralleling reduced Aβ uptake. A diacylglycerol acyltransferase 1 (DGAT1) inhibitor partially restored Aβ uptake in moderate-to-severe steatotic HepG2 cells. These findings provide cellular-level evidence for a stage-dependent relationship among LD remodeling, LRP1 alteration, and Aβ uptake in hepatocytes. Although the present results offer mechanistic clues at the cellular level, they do not fully capture the influence of the physiological microenvironment in vivo . Further animal studies will be required to determine whether these LD–LRP1–Aβ-associated changes are maintained under physiological conditions.
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2026
·
IF 10.5
Xuewen Peng,Sufen Yang,Zhengzhang Huang,Niu Feng,Hetong Zhang,Yiqi Li,Yating Han,Caiyang Zhang,Jimei Ma,Yiping Chen
Pathogens have long presented a significant threat to human lives and environmental security, and the rapid detection of infectious pathogens is therefore vital for mitigating such risks. However, the practical application of biosensors is often hampered by complex sample matrices and limited sensitivity. To address these challenges, we established magnetic nanoparticle (MNP) functionalized with phenylboronic acid (PBA) (MNP-PBA) that efficiently and non-destructively captures Staphylococcus aureus ( S. aureus ) from complex samples within 20 min, achieving 92.21% capture rate at 10 8 CFU/mL. In addition, an aggregation-induced emission luminogen, TTQ-PF 6 , was introduced as a detection probe. TTQ-PF 6 exhibits a large Stokes shift (265 nm), intense red fluorescence, and exceptional anti-photobleaching ability—representing a 5.67-fold and 9.40-fold enhancement compared to Rhodamine X, respectively. Moreover, TTQ-PF 6 allows multiple fluorophores to be conjugated to a single DNA strand without the need for chemical reagents. The combination of MNP-PBA and red-emitting TTQ-PF 6 provides strong resistance to matrix interference in bacterial detection, realizing a broad detection linear range for S. aureus from 10 2 to 10 7 CFU/mL, with a limit of detection as low as 13.74 CFU/mL. Overall, this work provides a robust and efficient platform for pathogen detection in complex environments.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2025
·
IF 4.7
Yong Huang,Huijiao Song,Peisheng Zhang,Chenyi Jiang,Jiahao Chen,Xin Xie,Yanqiong Peng,Ying Pan,Jianqiang Liu
Sulfur dioxide (SO 2 ) and viscosity play pivotal roles in regulating the physiological function within organisms. The abnormal levels of SO 2 and viscosity can lead to various diseases, emphasizing the need for reliable detection methods in biological and real food samples. Herein, we introduce a lipid droplet-targeted novel triphenylamine-based fluorescent probe, TPA-CN , which exhibits aggregation-induced emission (AIE) properties for dual response to SO 2 and viscosity. The TPA-CN could ratiometrically recognize SO 2 with several advantages, such as excellent selectivity and anti-interference, rapid response rate, good sensitivity, and wide pH adaptability. The TPA-CN was also applied to monitor the changes in viscosity through the twisted intramolecular charge transfer (TICT) mechanism. In addition, bioimaging results demonstrated that TPA-CN can effectively monitor endogenous and exogenous SO₂ as well as viscosity in real time, with specific targeting of lipid droplets. Moreover, TPA-CN facilitates determining SO 2 levels in water and food samples, demonstrating robust recovery performance.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Feng Wu,Yu Huang,Xian Yang,Jing-Jing Hu,Xiaoding Lou,Fan Xia,Yanlin Song,Lei Jiang
Determining the expression level of biomarkers is crucial for disease diagnosis. However, the low abundance of biomarkers in the early stage makes the detection extremely difficult by traditional aggregation-induced emission (AIE)-based fluorescent probes. Here, by tuning the intermolecular interaction, a two steps-based MP/NPs-SLIPS sensing system is designed for ultrasensitive detection of the tumor marker matrix metalloproteinase-2 (MMP-2). During the sensing process, aggregation of AIE residual could be intensified through the electrostatic absorption by negatively charged nanoparticles (NPs), as well as the confined space formed by the self-assembly of NPs to photonic crystals (PCs) on slippery lubricant-infused porous substrates (SLIPS). The fluorescent signals obviously increased with a strengthened aggregation degree, which contributes to improved sensitivity. Thus, the limit of detection is decreased to 3.7 ng/mL for MP/NPs-SLIPS sensing system, which could be used for detecting the MMP-2 secreted by tumor cells directly. This strategy also demonstrated its potential applications as high-throughput detection devices and will be of significance for the ultrasensitive analysis of biomarkers.
研究领域:
生物化学
·
医学诊断
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）