ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Xiancheng Wan,Huili Wang,Qiuhui Qian,Jin Yan
As a commonly used antibacterial agent in daily consumer products, triclosan (TCS) has attracted significant attention due to its potential environmental risks. In this study, we investigated the toxic effects of TCS exposure (1.4 μM) on heart development in zebrafish embryos. Our findings revealed that TCS exposure caused significant cardiac dysfunction, characterized by pericardial edema, malformations in the heart structure, and a slow heart rate. Additionally, TCS exposure induced oxidative damage and abnormal apoptosis in heart cells through the up-regulation of β-adrenergic receptor (β-AR) signaling pathway genes ( adrb1 , adrb2a , arrb2b ), similar to the effects induced by β-AR agonists. Notably, the adverse effects of TCS exposure were alleviated by β-AR antagonists. Using high-throughput transcriptome miRNA sequencing and targeted miRNA screening, we focused on miR-133b, which targets adrb1 and was down-regulated by TCS exposure, as a potential contributor to TCS-induced cardiotoxicity. Inhibition of miR-133b produced similar toxic effects as TCS exposure, while overexpression of miR-133b down-regulated the β-AR signaling pathway and rescued heart defects caused by TCS. In summary, our findings provide new insights into the mechanisms underlying the cardiotoxic effects of TCS. We suggest that targeting the β-AR pathway and miR-133b may be effective strategies for pharmacotherapy in cardiotoxicity induced by environmental pollutants such as TCS.
研究领域:
毒理学
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心脏病学
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分子生物学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
NANO
·
2020
·
IF 1.566
Yu Zhao,Bo Pang,Jie Chen,Ruyue Yan,Yang Zhang,Hou Liu,Yingnan Jiang,Wenhui Lian,Tianxia Sun,Zhe Zhang,Quan Lin
In this study, positively charged polystyrene- co -poly (ar-vinylbenzyl) trimethylammonium nanoparticles loading with Aggregation Induced Emission (AIE) fluorescent molecule sulfonated 9,10-distyrylanthracene derivatives (SDSA) by coulomb force denoted as SDSA/PS- co -PVBTAC have been prepared. The size, size distribution and morphology of nanoparticles have been tested by Dynamic light scattering (DLS) and Scanning electron microscope (SEM). The synthesized nanoparticles possess uniform size, good monodispersity and low biological toxicity. The AIE-type SDSA/PS- co -PVBTAC showed good fluorescence stability at pH from 3 to 10. It has been proved that the biological macromolecules including Bovine Serum Albumin (BSA), DNA and RNA can amplify the cell imaging signals. Moreover, the AIE-type nanoparticles have been shown to perform fluorescent imaging of various cells. It suggests that the prepared SDSA/PS- co -PVBTAC can be used as a new biological probe in the field of cell imaging and detection, as well as expanding the application of AIE fluorescent molecules in biosensing field.
研究领域:
生物化学
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材料科学
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细胞生物学
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产品关联
DOI
高影响因子
JOURNAL OF INORGANIC BIOCHEMISTRY
·
2019
·
IF 3.224
Jian Zhao,Nannan Liu,Shuchen Sun,Shaohua Gou,Xinyi Wang,Zhimei Wang,Xiaoyan Li,Wenjing Zhang
Targeted delivery of clinically approved anticancer drug to tumor sites is an effective way to achieve enhanced drug efficacy as well as reduced side effects and toxicity. Here bicalutamide is caged by the Ru(II) center through the nitrile group, and three photoactive Ru(II) complexes were designed and synthesized. Docking study showed that the ruthenium(II) fragments can effectively block the binding of complexes 1–3 with AR (androgen receptor) owing to the large steric structures , thus bicalutamide in complexes 1–3 could not interact with AR-LBD (ligand binding domain). Once irradiation with blue light (465 nm), complexes 1–3 can release bicalutamide and anticancer Ru(II) fragments, which possesses dual-action of AR binding and DNA interaction simultaneously. In vitro cytotoxicity study on these complexes further confirmed that complexes 1–3 exhibited considerable cytotoxicity upon irradiation with blue light. Significantly, complex 3 could be activated at 660 nm, which greatly increases the scope of complex 3 to treat deeper within tissue. Theoretical calculations showed that the lowest singlet excitation energy of complex 3 is lower than those of complexes 1–2 , which explains the experimental results well. Moreover, the 3 MC (metal centered) states of these complexes are more stable than their 3 MLCT (metal to ligand charge transfer) states, indicating that the photoactive processes of these complexes are likely to result in ligand dissociation.
研究领域:
化学
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药学
·
肿瘤学
·
光化学
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产品关联
DOI
PubMed
高影响因子
Journal of Translational Medicine
·
2023
·Vol. 21
·期 1
·1-14
·
IF 7.4
Chen Shengnan,Li Bo,Chen Lei,Jiang Hongli
Background Diabetic kidney disease (DKD) has been the leading cause of chronic kidney disease in developed countries. Evidence of the benefits of resveratrol (RES) for the treatment of DKD is accumulating. However, comprehensive therapeutic targets and underlying mechanisms through which RES exerts its effects against DKD are limited.Methods Drug targets of RES were obtained from Drugbank and SwissTargetPrediction Databases. Disease targets of DKD were obtained from DisGeNET, Genecards, and Therapeutic Target Database. Therapeutic targets for RES against DKD were identified by intersecting the drug targets and disease targets. GO functional enrichment analysis, KEGG pathway analysis, and disease association analysis were performed using the DAVID database and visualized by Cytoscape software. Molecular docking validation of the binding capacity between RES and targets was performed by UCSF Chimera software and SwissDock webserver. The high glucose (HG)-induced podocyte injury model, RT-qPCR, and western blot were used to verify the reliability of the effects of RES on target proteins.Results After the intersection of the 86 drug targets and 566 disease targets, 25 therapeutic targets for RES against DKD were obtained. And the target proteins were classified into 6 functional categories. A total of 11 cellular components terms and 27 diseases, and the top 20 enriched biological processes, molecular functions, and KEGG pathways potentially involved in the RES action against DKD were recorded. Molecular docking studies showed that RES had a strong binding affinity toward PPARA, ESR1, SLC2A1, SHBG, AR, AKR1B1, PPARG, IGF1R, RELA, PIK3CA, MMP9, AKT1, INSR, MMP2, TTR, and CYP2C9 domains. The HG-induced podocyte injury model was successfully constructed and validated by RT-qPCR and western blot. RES treatment was able to reverse the abnormal gene expression of PPARA, SHBG, AKR1B1, PPARG, IGF1R, MMP9, AKT1, and INSR.Conclusion sRES may target PPARA, SHBG, AKR1B1, PPARG, IGF1R, MMP9, AKT1, and INSR domains to act as a therapeutic agent for DKD. These findings comprehensively reveal the potential therapeutic targets for RES against DKD and provide theoretical bases for the clinical application of RES in the treatment of DKD.
研究领域:
药理学
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分子生物学
·
糖尿病
·
肾病学
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产品关联
DOI
PubMed
高影响因子
CARBON
·
2020
·
IF 8.821
Kunlin Liu,Ting Yang,Xuewen Zheng,Chengyang Wang,Mingming Chen
Low energy density is a fatal disadvantage of electric double-layer capacitor (EDLC). The improvement of the operation voltage of EDLCs is a more effective method to increase the energy density of EDLCs. When the operation voltage of EDLCs is higher than 2.7 V, the oxygen-containing functional groups on the surface of electrode materials (porous carbon) will decompose or chemically react with the electrolyte, causing significant deterioration of EDLC performance. In this paper, porous carbon with low oxygen content is prepared by using the potassium-assisted carbonization of chlorobenzene in Ar/H 2 mixed gas to improve withstanding voltage characteristic of porous carbon. By constructing a relatively closed and stable pyrolysis environment (employing the nickel crucible with a lid), using the carbonaceous precursor without oxygen atoms (chlorobenzene) and selecting reasonable carbonization operation atmosphere (Ar/H 2 ), the oxygen content of the as-prepared porous carbon (PC-KC-1000) is only 4.02 wt%. When the PC-KC-1000 sample is used as the electrode material of EDLCs, the operation voltage of EDLCs in the TEABF 4 /PC and EMIMBF 4 electrolytes is respectively 3.3 and 3.5 V, and the maximum energy density is respectively 53.5 and 64.4 Wh kg −1 . This strategy provides a new idea for the preparation of porous carbon with low oxygen content.
研究领域:
材料科学
·
能源存储
✓ 已验证
产品关联
DOI
高影响因子
Journal of Asthma and Allergy
·
2022
·
IF 3.027
Yang Sun,Tiancong Liu,Weiliang Bai
Purpose This work aimed to investigate the effects of MAF bZIP transcription factor B (MAFB) on the progression of allergic rhinitis (AR). Patients and Methods Nasal mucosa was isolated from AR patients and healthy individuals from Shengjing Hospital of China Medical University. The experimental procedures were approved by the Medical Ethics Committee of Shengjing Hospital of China Medical University (2019PS341K) in accordance with the Declaration of Helsinki. Informed consents were signed by participants or a parent/legal guardian of the participants under 18 years old of age. Then, an AR mouse model with MAFB overexpression was established with 25 μg ovalbumin (OVA) sensitization on day 0, 7, 14, followed by an injection with 1×10 7 TU/mL lentivirus MAFB on day 19 and a nasal challenge with 500 μg OVA from day 21 to 27. Results The results revealed that MAFB was down-regulated in the nasal mucosa of AR patients. The up-regulation of MAFB protected the AR mice against the OVA-induced allergic symptoms (sneezing and nasal rubbing) by alleviating the OVA-induced epithelial thicknesses, goblet cell hyperplasia, and inflammation including the eosinophil and mast cell infiltration. Moreover, MAFB facilitated the T helper (Th) 1 response and inhibited the Th2 and Th17 responses by the down-regulation of T-box transcription factor 21 and the up-regulation of GATA binding protein-3 as well as retinoid-related orphan receptor-γt in the splenocytes of AR mice. MAFB was found to repress the differentiation of naive CD4 + T cells into Th2 cells. Subsequently, MAFB overexpression reversed the OVA-induced enhancement of epithelial permeability, downregulation of tight junctions, and upregulation of cadherin-26, indicating the protective role of MAFB on epithelial barrier integrity. Conclusion MAFB protected against OVA-induced AR via the alleviation of inflammation by restoring the Th1/Th2/Th17 imbalance and epithelial barrier dysfunction.
研究领域:
免疫学
·
分子生物学
·
转化医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COMBUSTION AND FLAME
·
2024
·
IF 5.8
Peihui Xu,Xingang Dong,Wenke Zhang,Yuxin Yang,Xueqin Liao,Jianzhong Liu
Overcoming the difficulty of ignition and combustion of aluminium in water vapour is the key to being applied in underwater propellant applications. The effect of coating or blending 20 mass% ammonium perchlorate (namely, AP@Al or AP–Al samples) on the ignition and combustion of Al particles in a 1.0 MPa water vapour–Ar environment was investigated using a constant temperature pressurized combustion chamber and a CO 2 laser. The results show that the combustion of Al particles in a water vapour–Ar environment is a non-homogeneous reaction and that agglomeration processes of Al droplets are observed. The ignition and combustion properties of the AP@Al and AP–Al samples under water vapour were greatly improved due to the decomposition of AP for heat/oxygen (particularly, O 2 provided a new pathway for AlO(g) generation) supply and the effect of dispersed particles. The maximum pressure change and combustion temperature ( T max ) were increased significantly for the AP@Al and AP–Al samples combustion, while their ignition delay time ( t i ) and combustion time ( t c ) decreased remarkably. In addition, the coating form is more effective in the combustion-promoting effect of AP than the blending form. Interestingly, the increase in water vapour concentration is not conducive to the ignition and combustion of samples in this work. It is probably because the reaction system is lean-fuel where the excess water vapour absorbs some of the heat and prevents effective collisions among the radicals. In addition, the chemical equilibrium largely influences the combustion temperature, resulting in a conspicuous effect of changes in water vapour concentration on the T max values. The reaction interface largely influences the combustion reaction rate of Al, so changes in particle size have a significant effect on the t i and t c values.
研究领域:
材料科学
·
燃烧学
✓ 已验证
产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2018
·
IF 4.61
Sharafat Ali,Izaz Ali Shah,Aziz Ahmad,Javed Nawab,Haiou Huang
In this study, pristine multi-walled carbon nanotubes (MWCNTs) were functionalized by using Ar/O 2 plasma treatment technique, which enhanced adsorptive membrane filtration of zinc ions from water and wastewater. The XPS analysis showed that plasma treatment largely increased the surface oxygen groups content of MWCNTs from 2.78% to 6.79%. This change increased the surface negative charged, dispersion and adsorption properties of MWCNTs without causing any damages to the integrity of the nanotube pattern. Pressure-driven filtration of plasma-treated MWCNT (P-CNT) dispersion formed a stable layer inside the lumen of a hollow fiber membrane. The contact angle analysis demonstrated that after incorporation of P-CNT into the HF membrane increased membrane hydrophilicity. The P-CNT membrane effectively removed almost 100% of zinc from synthetic waters and approximately 80% of zinc from a wastewater effluent by surface complexation reaction. A follow-up regeneration study demonstrated that the adsorptive removal of zinc by the CNT membrane was reversible under selected conditions, thus making it possible to repeatedly use the membrane for long-term zinc filtration. This study suggests that HF membranes modified with P-CNTs possess superb adsorption properties for metal ions , allowing the operation of CNT membranes for water treatment .
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMER DEGRADATION AND STABILITY
·
2025
·
IF 7.4
Renjian Xie,Xiu-e Li,Xiaohong Liu,Jialin Li,Shanshan Zhou,Yumei Li,Hui Yang,Guoming Yuan,Kun Wu,Kunxin Wang
Black phosphorous (BP) nanosheets as a novel two-dimensional material have revealed dramatically fire safety promotion in polymer nanocomposite. Herein, plasma cleaning machine (PC), equiped with Ar plasma, was applied to protect BP (PCBP) to enhance the environmental stability of BP. Further, the fire safety of epoxy resin (EP) was enhanced. The molecular dynamics simulation (MDS) results indicate that the values of binding energy between Ar plasma and BP at 0 K, 200 K, 400 K, 600 K, and 800 K were -847.0148, -847.3044, -847.3012, -847.2975, and -847.3035 eV, respectively. It meant that there was a strong electrostatic interaction between BP and Ar plasma, and it was not affected by temperature. Electron Paramagnetic Resonance (EPR) test revealed that the free radical strength of PCBP decreased than BP, suggesting that the environmental stability of PCBP was better than that of BP. The results of cone calorimetry test (CCT) displayed that the flame retardant ability of EP was enormously improved through the addition amount of 1.5 wt% PCBP nanosheets, for instance, the peak heat release rate (pHRR) and the total heat release (THR) were descended by 22.98 % and 16.24 %, respectively. Epoxy nanocomposite with 1.5 wt% PCBP nanosheets can achieve UL-94 V-0 rating level with a limit oxygen index (LOI) of 30.4. Thermogravimetric analysis (TGA) result displayed that the residual weight rate increased by 23.27 %. Raman test of the residual char layer proved that the value of I D /I G declined from 4.021 to 3.098, indicating a promotion in the degree of graphitization. These were attributable to the cooperation effect of physical barrier, free radicals trapping and catalytic conversion into char of PCBP.
研究领域:
材料科学
·
高分子化学
·
消防安全
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
MATERIALS SCIENCE IN SEMICONDUCTOR PROCESSING
·
2026
·
IF 5.2
Gui Chen,Yun-Hao Shao,Wen Zhu,Xin-Ping Qu
The oxidation of the Cu surface remains the most significant challenge of low-temperature Cu-Cu bonding technology. Schemes to achieve and maintain a high-quality Cu surface prior to bonding are therefore urgently needed. Co layer was used as a passivation layer and a simple wet treatment method using glycine was then proposed to remove surface oxides from Co metal. In this work, a novel simulated bonding methodology is proposed, which utilizes a two-step PVD Cu/Co on the PVD Co/Cu surface with a vacuum break, followed by annealing to simulate and investigate bonding mechanisms. The reliability of this methodology was first rigorously validated using Ar plasma activation as a benchmark, yielding simulated specific contact resistance results that closely align with actual bonding data. The simulation bonding methodology was employed to systematically optimize the process, after which the bonding mechanism was investigated. Consequently, chip-level bonding was successfully achieved at 230 °C, exhibiting an ultra-low specific contact resistance of 2.9 × 10 −8 Ω·cm 2 with a bonding area exceeding 99%. HRTEM and EDS analyses confirm that glycine treatment promotes direct Co-to-Co contact and uniform atomic interdiffusion. This methodology provides an efficient platform for investigating interfacial treatment processes and accelerating the development of advanced 3D integration technology.
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Medical Sciences
·
2017
·
IF 2.399
Lin Yu,Xiaofeng Wu,Min Chen,Huarong Huang,Yan He,Huaqian Wang,Dongli Li,Zhiyun Du,Kun Zhang,Susan Goodin,Xi Zheng
Background : Docetaxel is the first-line treatment for castration-resistant prostate cancer (CRPC). The limited survival benefit associated with the quick emergence of resistance and systemic toxicity diminishes its efficacy in high-dose monotherapy. YK-4-279 is a small molecule inhibitor of ETV1 that plays an important role in the progression of prostate cancer. The aim of this study was to evaluate the hypothesis that the combination of docetaxel and YK-4-279 will have a synergistic effect on inhibiting growth and accelerating apoptosis in human prostate cancer cells. Methods : Cell growth assessed using CCK-8 and trypan blue exclusion assays. Cell apoptosis was determined by morphological assessment in cells stained with propidium iodide. Standard scratch migration and Matrigel-coated transwell invasion assays were used to assess cell migration and invasion, respectively. Western blotting was used to investigate the levels of ETV1, AR, PSA, p-STAT3, survivin, Bcl-2, and p-Akt in prostate cancer cells. Results : The combination of low-dose docetaxel and YK-4-279 synergistically inhibited growth and induced apoptosis in human prostate cancer cells. The combination also more efficiently suppressed the migration and invasion of LNCaP and PC-3 cells. The combination of low-dose docetaxel and YK-4-279 caused a stronger decrease in the levels of ETV1, AR, PSA, p-STAT3, survivin, Bcl-2, and p-Akt in LNCaP cells and of p-Akt, Bcl-2, and p-STAT3 in PC-3 cells compared with either drug alone. Conclusions : These data suggest that the combination of docetaxel and YK-4-279 may be an effective approach for inhibiting the growth and metastasis of prostate cancer. This could permit a decrease in the docetaxel dose necessary for patients with CRPC and thereby lower its systemic toxicity.
研究领域:
肿瘤学
·
药理学
·
分子生物学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INFLAMMATION
·
2025
·
IF 5
Deng Qirui,Liu Xinyan,Wen Xiujuan,Huang Hao,Tang Hongfeng
The aim of this study was to investigate how ultraviolet B (UVB) light regulates AP-1 expression via the β2-adrenergic receptor (β2-AR) in epidermal keratinocytes, which in turn regulates melanin synthesis in melanocytes, thereby modulating downstream melanin production in skin hair follicles and altering mouse skin color. We established a UV-irradiated mouse model to investigate the effects of UV radiation on changes in skin color. By measuring changes in the expression of genes related to cutaneous sympathetic nerves, norepinephrine synthesis and melanin synthesis, we investigated the relationship between β2-AR expression and cutaneous melanogenesis and determined the localization of β2-AR in cells. The results of the siRNA-mediated transfection of keratinized cells with downregulated β2-AR expression were further verified in vitro. Our results suggest that UVB alters the color of the dorsal skin in mice by activating the AP-1/IL-6 pathway, which triggers the sympathetic release of norepinephrine, thereby increasing β2-AR expression in keratinocytes. Overall, our study improves the current understanding of how UVB light influences skin color changes and highlights the complex interplay between ultraviolet radiation and skin physiology.
研究领域:
皮肤病学
·
分子生物学
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）